SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS04460 FitnessBrowser__Cup4G11:RR42_RS04460 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 16 hits to proteins with known functional sites (download)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
32% identity, 92% coverage: 28:377/379 of query aligns to 7:377/386 of P0A9J8

query
sites
P0A9J8

L

L

T

L

P

A

L

L

R
|
R

E

E

Q

K

I

I

D

S

T

A

L

L

D

D

R

E

E

K

L

L

A

A

M

L

L

L

N

A

R

E

R
|
R

A

R

Q

E

L

L

A

A

L

V

D

E

V

V

G

G

E

K

V

A

K
|
K

K

L

Y

S

G

H

A

R

P

P

V

V

F

R

R

D

P

I

E

D

R

R

E
|
E

L

R

Q

D

V

L

I

L

R

E

K

R

V

L

Q

I

G

T

A

L

N

G

P

K

G

A

P

H

L

H

L

L

D

D

D

A

S

H

V

Y

A

I

A

T

-

R

-

L

-

F

-

Q

-

L

-

I

I

I

W

E

R

D

E

S

V

V

M

L

S

T

A
x
Q

C

Q

R

A

G

L

L

L

E

Q

K

Q

P

H

L

L

E

N

-

K

-

I

-

N

-

P

-

H

-

S

-

A

-

R

V

I

A

A

F

F

L

L

G

G

P

P

A

K

G

G

T

S

F

Y

S

S

E

H

Q

L

A

A

L

A

-

R

-

Q

Y

Y

A

A

-

A

-

R

H

H

F

F

G

E

H

Q

E

F

V

I

-

E

S

S

G

G

V

-

P

-

C

C

P

A

S

K

I

F

D

A

E

D

V

I

F

F

R

N

A

Q

V

V

E

E

A

T

G

G

T

Q

V

A

E

D

Y

Y

G

A

V

V

P

P

V

I

E

E

N

N

S

T

T

S

E

S

G

G

A

A

V

I

S

N

R

D

T

V

L

Y

D

D

L

L

F

L

L

Q

Q

H

T

T

S

S

L

L

K

S

I

I

S

V

G

G

E

E

I

M

A

T

L

L

K

T

V

I

H

D

H

H

N

C

L

L

M

L

A

V

S

S

-

G

S

T

P

T

D

D

M

L

K

S

G

T

V

I

T

N

V

T

V

V

R

Y

A

S

H

H

A

P

Q

Q

A

P

L

F

A

Q

Q

Q

C

C

Q

S

H

K

W

F

L

L

T

N

A

-

N

R

Y

Y

P

P

H

H

L

W

E

K

R

I

Q

E

A

Y

V

T

S

E

S

S

N

T

A

S

E

A

A

A

A

M

R

E

M

K

A

V

S

A

E

Q

-

A

-

K

D

S

P

P

T

H

V

V

A

A

I

L

A

G

G

S

E

E

S

A

A

G

N

T

R

L

Y

Y

H

G

L

L

H

Q

L

V

V

L

R

E

T

R

H

I

I

E

Q

A

D

N

D

Q

P

R

H

Q

N

N

R

F

T

T

R

R

F

F

A

V

V

V

I

L

G

A

R

R

Y

K

E

A

T

I

E

N

P

V

S

S

G

D

S

Q

-

V

-

P

D

A

Q

K

T

T

S

T

M

L

I

L

L

M

S

A

V

T

P

G

N

Q

K

Q

A

A

G

G

A

A

V

L

Y

V

Q

E

L

A

L

L

A

L

P

V

L

L

A

R

D

N

N

H

G

N

V

L

S

I

M

M

C

T

R

R

F

L

E

E

S

S

R

R

P

P

A

I

R

H

S

G

G

N

A

P

W

W

E

E

Y

E

Y

M

F

F

Y

Y

V

L

D

D

V

I

E

Q

G

A

H

N

Q

L

H

E

D

S

A

A

S

E

V

M

A

Q

R

K

A

A

L

L

E

K

E

E

L

L

R

G

R

E

N

I

A

T

A

R

Y

S

F

M

K

K

V

V

L

L

G

G

S

C

Y

Y

P

P

S

S

R11 (= R32) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R49) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K60) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E73) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (≠ A103) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

2qmxA The crystal structure of l-phe inhibited prephenate dehydratase from chlorobium tepidum tls (see paper)
36% identity, 69% coverage: 113:375/379 of query aligns to 4:273/278 of 2qmxA

query
sites
2qmxA

V

I

A

A

F

Y

L

Q

G

G

P

E

A

P

G

G

T

A

F

Y

S

S

E

E

Q

I

A

A

L

A

Y

L

A

-

H

R

F

F

G

G

H

E

E

P

V

L

S

-

G

-

V

-

P

P

C

C

P

E

S

S

I

F

D

D

E

D

V

V

F

F

R

S

A

A

V

V

E

T

A

E

G

Q

T

K

V

A

E

D

Y

Y

G

A

V

V

V

I

P

P

V

I

E

E

N

N

S

S

T

L

E

G

G

G

A

S

V

I

S

H

R

Q

T

N

L

Y

D

D

L

L

F

L

L

L

Q

R

T

R

S

P

L

V

K

V

I

I

S

L

G

A

E

E

I

T

A

F

L

V

K

K

V

V

H

E

H

H

N

C

L

L

M

L

A

G

-

L

S

P

S

G

P

A

D

S

M

V

K

E

G

T

V

A

T

T

V

K

V

A

R

M

A

S

H

H

A

P

Q

Q

A

A

L

L

A

V

Q

Q

C

C

Q

H

H

N

W

F

L

F

T

-

A

A

N

T

Y

H

P

P

H

Q

L

I

E

R

R

A

Q

E

A

A

V

A

S

Y

S

D

N

T

A

A

E

G

A

S

A

A

R

K

M

M

A

V

S

A

E

E

-

S

-

R

D

D

P

K

T

S

V

A

A

L

A

A

I

I

A

A

G

S

E

K

S

R

A

A

A

G

N

E

R

L

Y

Y

H

G

L

L

H

D

L

I

V

L

R

K

T

E

H

N

I

L

Q

A

D

D

D

E

P

E

H

W

N

N

R

I

T
|
T

R

R

F
|
F

A

F

V

C

I

I

G

A

R

-

Y

H

E

E

T

N

E

N

P

P

S

D

G

I

S

S

-

H

-

L

-

K

-

V

-

R

-

P

-

D

-

V

-

A

-

R

D

Q

Q

K

T

T

S

S

M

I

I

V

L

F

S

A

V

L

P

P

N
|
N

K
x
E

A

Q

G

G

A

S

V
x
L

Y

F

Q

R

L

A

L

L

A

A

P

T

L

F

A

A

D

L

N

R

G

G

V

I

S
x
D

M
x
L

C
x
T

R
x
K

F

I

E

E

S
|
S

R

R

P

P

A

S

R

R

S

K

G

K

A

A

W

F

E

E

Y
|
Y

Y

L

F
|
F

Y

Y

V

A

D

D

V

F

E

I

G

G

H

H

Q

R

H

E

D

D

A

Q

S

N

V

V

A

H

R

N

A

A

L

L

E

E

E

N

L

L

R

R

R

E

N

F

A

A

A

T

Y

M

F

V

K

K

V

V

L

L

G

G

S

S

Y

Y

active site: T169 (= T280), F171 (= F282)
binding phenylalanine: N204 (= N306), E205 (≠ K307), L209 (≠ V311), D222 (≠ S324), L223 (≠ M325), T224 (≠ C326), K225 (≠ R327), S228 (= S330), Y238 (= Y340), F240 (= F342)

3mwbA The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
34% identity, 69% coverage: 115:375/379 of query aligns to 5:274/306 of 3mwbA

query
sites
3mwbA

F

F

L

L

G

G

P

P

A

Q

G

G

T

T

F

F

S

T

E

E

Q

A

A

A

L

L

Y

M

A

Q

H

V

F

P

G

G

H

A

-

A

E

D

V

A

S

T

G

R

V

I

P

P

C

C

P

T

S

N

I

V

D

N

E

T

V

A

F

L

R

E

A

R

V

V

E

R

A

A

G

G

T

E

V

A

E

D

Y

A

G

A

V

M

V

V

P

P

V

I

E

E

N

N

S

S

T

V

E

E

G

G

A

G

V

V

S

T

R

A

T

T

L

L

D

D

-

A

L

I

F

A

L

T

Q

G

T

Q

S

E

L

L

K

R

I

I

S

I

G

R

E

E

I

A

A

L

L

V

K

P

V

I

H

T

H

F

N

V

L

L

M

V

A

A

-

R

S

P

S

G

P

V

D

E

M

L

K

S

G

D

V

I

T

K

V

R

V

I

R

S

A

T

H

H

A

G

Q

H

A

A

L

W

A

A

Q

Q

C

C

Q

R

H

L

W

W

L

V

T

D

A

E

N

H

Y

L

P

P

H

N

L

A

E

D

R

Y

Q

V

A

P

V

G

S

S

N

T

A

A

E

A

A

S

A

A

R

M

M

G

A

L

S

L

E

E

D

D

-

D

-

A

P

P

T

Y

V

E

A

A

I

I

A

C

G

A

E

P

-

L

S

I

A

A

N

E

R

Q

Y

P

H

G

L

L

H

N

L

V

V

L

R

A

T

E

H

D

I

I

Q

G

D

D

D

N

P

P

H

D

N

A

R

V

T
|
T

R

R

F
|
F

A

I

V

L

I

V

G

S

R

R

Y

P

E

G

T

A

E

L

P

P

-

E

-

R

S

T

G

G

S

A

D

D

Q

K

T

T

S

T

M

V

I

V

L

V

S

P

V

L

P

P

-

E

N
x
D

K
x
H

A

P

G

G

A

A

V
x
L

Y

M

Q

E

L

I

L

L

A

D

P

Q

L

F

A

A

D

S

N

R

G

G

V

V

S
x
N

M
x
L

C
x
S

R
|
R

F

I

E

E

S
|
S

R

R

P

P

A

T

R

G

S

Q

G

Y

A

L

W

G

E

H

Y

Y

Y

F

F
|
F

Y

S

V

I

D

D

V

A

E

D

G

G

H

H

Q

A

H

T

D

D

A

S

S

R

V

V

A

A

R

D

A

A

L

L

E

A

E

G

L

L

R

H

R

R

N

I

A

S

A

P

Y

A

F

T

K

R

V

F

L

L

G

G

S

S

Y

Y

active site: T176 (= T280), F178 (= F282)
binding phenylalanine: D205 (≠ N306), H206 (≠ K307), L210 (≠ V311), N223 (≠ S324), L224 (≠ M325), S225 (≠ C326), R226 (= R327), S229 (= S330), F241 (= F342)

3mwbB The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
34% identity, 69% coverage: 115:375/379 of query aligns to 5:271/303 of 3mwbB

query
sites
3mwbB

F

F

L

L

G

G

P

P

A

Q

G

G

T

T

F

F

S

T

E

E

Q

A

A

A

L

L

Y

M

A

Q

H

V

F

P

G

G

H

A

-

A

E

D

V

A

S

T

G

R

V

I

P

P

C

C

P

T

S

N

I

V

D

N

E

T

V

A

F

L

R

E

A

R

V

V

E

R

A

A

G

G

T

E

V

A

E

D

Y

A

G

A

V

M

V

V

P

P

V

I

E

E

N

N

S

S

T

V

E

E

G

G

A

G

V

V

S

T

R

A

T

T

L

L

D

D

-

A

L

I

F

A

L

T

Q

G

T

Q

S

E

L

L

K

R

I

I

S

I

G

R

E

E

I

A

A

L

L

V

K

P

V

I

H

T

H

F

N

V

L

L

M

V

A

A

-

R

S

P

S

G

P

V

D

E

M

L

K

S

G

D

V

I

T

K

V

R

V

I

R

S

A

T

H

H

A

G

Q

H

A

A

L

W

A

A

Q

Q

C

C

Q

R

H

L

W

W

L

V

T

D

A

E

N

H

Y

L

P

P

H

N

L

A

E

D

R

Y

Q

V

A

P

V

G

S

S

N

T

A

A

E

A

A

S

A

A

R

M

M

G

A

L

S

L

E

E

D

D

-

D

-

A

P

P

T

Y

V

E

A

A

I

I

A

C

G

A

E

P

-

L

S

I

A
|
A

A

A

N

E

R
x
Q

Y

P

H

G

L
|
L

H

N

L

V

V

L

R

A

T

E

H

D

I

I

Q

G

D

D

D

N

P

P

H

D

N

A

R

V

T
|
T

R

R

F
|
F

A

I

V

L

I

V

G

S

R

R

Y

P

E

G

T

A

E

L

P

P

-

E

-

R

S

T

G

G

S

A

D

D

Q

K

T

T

S

T

M

V

I

V

L

V

S

P

V

L

P

P

-

E

N
x
D

K
x
H

A

P

G

G

A

A

V
x
L

Y

M

Q

E

L

I

L

L

A

D

P

Q

L

F

A

A

D

S

N

R

G

G

V

V

S
x
N

M
x
L

C
x
S

R
|
R

F
x
I

E

E

S

S

R

R

P

P

A

T

R

L

S

G

G

-

A

-

W

-

E

H

Y
|
Y

Y

F

F
|
F

Y

S

V

I

D

D

V

A

E

D

G

G

H

H

Q

A

H

T

D

D

A

S

S

R

V

V

A

A

R

D

A

A

L

L

E

A

E

G

L

L

R

H

R

R

N

I

A

S

A

P

Y

A

F

T

K

R

V

F

L

L

G

G

S

S

Y

Y

active site: T176 (= T280), F178 (= F282)
binding magnesium ion: A155 (= A259), Q158 (≠ R262), L161 (= L265)
binding phenylalanine: D205 (≠ N306), H206 (≠ K307), L210 (≠ V311), N223 (≠ S324), L224 (≠ M325), S225 (≠ C326), R226 (= R327), I227 (≠ F328), Y236 (= Y340), F238 (= F342)

6vh5D Crystal structure of prephenate dehydratase from brucella melitensis biovar abortus 2308 in complex with phenylalanine
31% identity, 72% coverage: 106:378/379 of query aligns to 2:279/282 of 6vh5D

query
sites
6vh5D

G

G

L

S

E

M

K

K

P

T

L

N

E

R

V

I

A

S

F

F

L

Q

G

G

P

E

A

A

G

G

T

A

F

N

S

S

E

D

Q

T

A

A

L

C

Y

R

A

N

H

M

F

F

G

-

H

P

E

D

V

M

S

E

G

P

V

L

P

P

C

C

P

P

S

T

I

F

D

E

E

D

V

A

F

F

R

N

A

A

V

V

E

E

A

T

G

G

T

A

V

A

E

D

Y

L

G

A

V

M

V

I

P

P

V

I

E

E

N

N

S

T

T

L

E

A

G

G

A

R

V

V

S

A

R

D

T

I

L

H

D

Y

L

L

F

L

L

P

Q

L

T

A

S

D

L

M

K

H

I

I

S

V

G

G

E

E

I

Y

A

F

L

L

K

P

V

I

H

H

H

F

N

Q

L

L

M

M

A

V

S

-

S

L

P

P

D

G

M

V

K

R

-

R

-

E

G

E

V

I

T

K

V

T

V

V

R

H

A

S

H

H

A

I

Q

H

A

A

L

L

A

G

Q

Q

C

C

Q

R

H

N

W

V

L

I

T

R

A

Q

N

N

Y

-

P

-

H

G

L

W

E

K

R

G

Q

V

A

I

V

A

S

G

S

D

N

T

A

A

E

G

A

A

R

R

M

L

A

V

S

A

E

D

-

V

-

K

D

D

P

R

T

S

V

M

A

A

I

L

A

A

G

P

E

R

S

L

A

A

N

D

R

L

Y

Y

H

G

L

L

H

D

L

I

V

L

R

E

T

E

H

N

I

V

Q

E

D

D

D

S

P

E

H

N

N

N

R

V

T

T

R

R

F

F

A

V

V

V

I

L

G

S

R

K

-

N

-

K

-

Q

Y

W

E

A

T

A

E

R

P

P

S

E

G

N

S

D

D

E

Q

R

-

I

-

V

T

T

S

T

M

F

I

V

L

F

S

R

V

V

P

R

N
|
N

K
x
V

A

P

G

A

A

A

V
x
L

Y

Y

Q

K

L

A

L

L

A

G

P

G

L

F

A

A

D

T

N

N

G

G

V

V

S
x
N

M
|
M

C
x
T

R
x
K

F

L

E

E

S
|
S

R

Y

P

Q

A

L

R

G

S

G

G

R

A

F

W

I

E

A

Y
x
T

Y

Q

F
|
F

Y

Y

V

A

D

D

V

I

E

E

G

G

H

H

Q

P

H

E

D

E

A

R

S

S

V

V

A

Q

R

L

A

A

L

L

E

E

L

L

R

R

R

F

N

F

A

T

A

K

Y

E

F

V

K

R

V

I

L

L

G

G

S

V

Y

Y

P

K

S

G

S

S

binding phenylalanine: N207 (= N306), V208 (≠ K307), L212 (≠ V311), N225 (≠ S324), M226 (= M325), T227 (≠ C326), K228 (≠ R327), S231 (= S330), T241 (≠ Y340), F243 (= F342)

7am0B Gqqa- a novel type of quorum quenching acylases (see paper)
31% identity, 70% coverage: 113:378/379 of query aligns to 4:272/278 of 7am0B

query
sites
7am0B

V

I

A

A

F

F

L

Q

G

G

P

R

A

P

G

G

T

A

F

Y

S

S

E

D

Q

L

A

A

L

C

-

R

Y

Q

A

A

H

R

F

P

G

G

H

W

E

-

V

-

S

T

G

T

V

L

P

P

C

C

P

Q

S

T

I

F

D

A

E

Q

V

T

F

I

R

A

A

A

V

V

E

H

A

D

G

G

T

R

V

A

E

E

Y

L

G

A

V

M

V

L

P

A

V

C

E

E

N

N

S

S

T

L

E

A

G

G

A

R

V

V

S

P

R

D

T

I

L

H

D

A

L

L

F

L

L

P

Q

E

T

A

S

G

L

L

K

F

I

I

S

V

G

G

E

E

I

H

A

F

L

Q

K

R

V

V

H

E

H

H

N

C

L

L

M

L

A

G

S

-

P

-

D

-

M

I

K

P

G

G

V

S

T

T

V

L

-

A

-

D

-

A

-

R

V

I

R

H

A

T

H

H

A

P

Q

V

A

A

L

M

A

A

Q

Q

C

V

Q

R

H

G

W

I

L

I

T

T

A

E

N

L

Y

-

P

-

H

G

L

L

E

D

R

P

Q

V

A

V

V

E

S

F

S

D

N

T

A

A

E

G

A

A

R

E

M

M

A

V

S

R

E

E

-

W

-

G

D

R

P

K

T

E

V

D

A

V

A

A

I

V

A

A

G

S

E

A

S

L

A

A

N

E

R

L

Y

N

H

G

L

L

H

E

L

I

V

L

R

R

T

R

H

N

I

V

Q

E

D

D

D

A

P

T

H

H

N

N

R

T

T

T

R

R

F

F

A

Y

V

I

I

A

G

S

R

R

Y

R

E

P

T

A

E

T

-

L

-

P

P

P

S

G

G

P

S

G

D

F

Q

M

T

T

S

T

M

L

I

L

L

F

S

R

V

V

P
x
N

N
|
N

K
x
Q

A
x
P

G

G

A

A

V
x
L

Y

Y

Q

K

L

A

L

L

A

G

P

G

L

L

A

A

D

T

N

A

G

G

V

V

S
x
N

M
|
M

C
x
T

R
|
R

F

L

E

E

S

S

R

Y

P

M

A

L

R

E

S

G

G

S

A

F

W

S

E

A

Y
x
T

Y

Q

F
|
F

Y

L

V

M

D

D

V

V

E

E

G

G

H

H

Q

P

H

E

D

A

A

P

S

P

V

L

A

A

R

R

A

A

L

L

E

D

E

E

L

L

R

S

R

F

N

F

A

S

A

E

Y

Q

F

Q

K

E

V

I

L

L

G

G

S

V

Y

Y

P

P

S

A

S

S

binding phenylalanine: N199 (≠ P305), N200 (= N306), Q201 (≠ K307), P202 (≠ A308), L205 (≠ V311), N218 (≠ S324), M219 (= M325), T220 (≠ C326), R221 (= R327), T234 (≠ Y340), F236 (= F342)

A0A0M0ELU2 Bifunctional N-acyl-homoserine lactone acylase/prephenate dehydratase; Bifunctional AHL acylase/PDT; Quorum quenching protein GqqA; EC 3.5.1.97; EC 4.2.1.51 from Komagataeibacter europaeus (Gluconacetobacter europaeus) (see paper)
31% identity, 70% coverage: 113:378/379 of query aligns to 7:275/281 of A0A0M0ELU2

query
sites
A0A0M0ELU2

V

I

A

A

F

F

L

Q

G

G

P

R

A

P

G

G

T

A

F

Y

S

S

E

D

Q

L

A

A

L

C

-

R

Y

Q

A

A

H
x
R

F

P

G

G

H

W

E

-

V

-

S

T

G

T

V

L

P

P

C

C

P

Q

S

T

I

F

D

A

E

Q

V

T

F

I

R

A

A

A

V

V

E

H

A

D

G

G

T

R

V

A

E

E

Y

L

G

A

V

M

V

L

P

A

V

C

E

E

N

N

S

S

T

L

E

A

G

G

A

R

V

V

S

P

R

D

T

I

L

H

D

A

L

L

F

L

L

P

Q

E

T

A

S

G

L

L

K

F

I

I

S

V

G

G

E

E

I

H

A

F

L

Q

K

R

V

V

H

E

H

H

N

C

L

L

M

L

A

G

S

-

P

-

D

-

M

I

K

P

G

G

V

S

T

T

V

L

-

A

-

D

-

A

-

R

V

I

R

H

A

T

H

H

A

P

Q

V

A

A

L

M

A

A

Q

Q

C

V

Q

R

H

G

W

I

L

I

T
|
T

A

E

N

L

Y

-

P

-

H

G

L

L

E

D

R

P

Q

V

A

V

V

E

S

F

S

D

N

T

A

A

E

G

A

A

R

E

M

M

A

V

S

R

E

E

-

W

-

G

D

R

P

K

T

E

V

D

A

V

A

A

I

V

A

A

G

S

E

A

S

L

A

A

N

E

R

L

Y

N

H

G

L

L

H

E

L

I

V

L

R

R

T

R

H

N

I

V

Q

E

D

D

D

A

P

T

H

H

N

N

R

T

T

T

R

R

F

F

A

Y

V

I

I

A

G

S

R

R

Y

R

E

P

T

A

E

T

-

L

-
x
P

P
|
P

S
x
G

G
x
P

S

G

D

F

Q

M

T

T

S

T

M

L

I

L

L

F

S

R

V

V

P

N

N

N

K

Q

A

P

G

G

A
|
A

V
x
L

Y

Y

Q

K

L

A

L

L

A

G

P

G

L

L

A

A

D

T

N

A

G

G

V

V

S
x
N

M
|
M

C

T

R

R

F

L

E

E

S

S

R

Y

P

M

A

L

R

E

S

G

G

S

A

F

W

S

E

A

Y

T

Y

Q

F

F

Y

L

V

M

D

D

V

V

E

E

G

G

H

H

Q

P

H

E

D

A

A

P

S

P

V

L

A

A

R

R

A

A

L

L

E

D

E

E

L

L

R

S

R
x
F

N

F

A

S

A

E

Y

Q

F

Q

K

E

V

I

L

L

G

G

S

V

Y

Y

P

P

S

A

S

S

R25 (≠ H130) mutation to S: Affects dimerization. Loss of N-acyl-homoserine lactone acylase activity.
T118 (= T224) mutation to S: Does not affect N-acyl-homoserine lactone acylase activity.
PPPGP 187:191 (≠ --PSG 292:294) mutation to AS: Loss of N-acyl-homoserine lactone acylase activity.
A207 (= A310) binding L-phenylalanine
L208 (≠ V311) binding L-phenylalanine
N221 (≠ S324) binding L-phenylalanine
M222 (= M325) binding L-phenylalanine
F261 (≠ R364) mutation to S: Loss of N-acyl-homoserine lactone acylase activity.

Sites not aligning to the query:

279:281 mutation Missing: Does not affect N-acyl-homoserine lactone acylase activity.

3luyA Putative chorismate mutase from bifidobacterium adolescentis
27% identity, 69% coverage: 115:376/379 of query aligns to 8:284/326 of 3luyA

query
sites
3luyA

F

Y

L

L

G

G

P

P

A

Q

G

G

T

T

F

F

S

T

E

H

Q

Q

A

A

L

A

-

V

-

N

-

A

-

Q

-

E

Y

L

A

A

H

R

F

F

-

E

-

P

-

Q

G

G

H

F

E

D

V

L

S

-

G

-

V

M

P

P

C

M

P

D

S

D

I

V

D

P

E

Q

V

I

F

L

R

D

A

A

V

A

E

Q

A

H

G

G

T

D

V

-

E

G

Y

W

G

G

V

I

V

V

P

A

V

W

E

E

N

N

S

N

T

V

E

E

G

G

A

Y

V

V

S

V

R

P

T

N

L

L

D

D

L

A

F

L

L

I

Q

D

T

A

S

K

L

D

K

L

I

V

S

G

-

F

G

A

E

R

I

V

A

G

L

V

K

N

V

V

H

E

H

F

N

D

-

A

L

Y

M

V

A

A

S

Q

S

G

P

A

D

D

M

P

K

A

G

E

V

A

T

R

V

I

V

A

R

T

A

A

H

H

A

P

Q

H

A

G

L

L

A

A

Q

Q

C

C

Q

K

H

R

W

F

L

I

T

A

A

E

N

H

Y

-

P

-

H

R

L

L

E

S

R

T

Q

Q

A

P

V

A

S

T

S

S

N

N

A

A

E

A

A

A

A

C

R

R

-

D

-

L

M

I

A

P

S

G

E

E

D

I

P

A

T

F

V

G

A

P

A

A

I

I

A

C

G

G

E

E

S

-

A

-

N

-

R

L

Y

Y

H

D

L

I

H

T

L

R

V

I

R

G

T

T

H

A

I

I

Q

Q

D

D

D

Y

P

Q

H

G

N

A

R

A

T
|
T

R

D

F

F

A

L

V

V

-

L

-

S

-

P

-

R

-

A

-

E

I

V

G

A

R

R

Y

L

E

L

T

A

E

K

P

P

-

R

-

A

-

E

S

A

G

N

S

V

D

E

Q

Y

T

E

S

S

M

V

I

L

L

T

S

L

V

I

P

P

-

L

-

V

N

T

K
x
G

A

P

G

G

A

V

V
x
L

Y

A

Q

N

L

L

A

D

P

V

L

F

A

R

D

D

N

A

G

G

V

L

S

N

M

M

C

T

R

S

F

F

E

I

S

S

R

R

P

P

A

I

R

K

S

G

G

R

A

T

W

G

E

T

Y
|
Y

Y

S

F
|
F

Y

I

V

V

D

T

V

L

E

D

G

A

H

A

Q

P

H

W

D

E

A

E

S

R

V

F

A

R

R

D

A

A

L

L

E

V

E

E

L

I

R

A

R

E

N

H

A

G

A

D

Y

W

F

A

K

K

V

T

L

L

G

A

S

V

Y

Y

P

P

active site: T177 (= T280)
binding 3-phenylpyruvic acid: G215 (≠ K307), L219 (≠ V311), Y248 (= Y340), F250 (= F342)

7alzA Gqqa- a novel type of quorum quenching acylases (see paper)
34% identity, 37% coverage: 239:378/379 of query aligns to 46:188/194 of 7alzA

query
sites
7alzA

A

A

E

E

A

L

A

A

R

M

M

L

A

A

S

C

E

E

D

N

-

S

-

L

-

A

-

G

-

R

-

V

P

P

T

D

V

I

A

H

A

A

I

L

A

L

G

P

E

E

S

A

A

G

A

-

N

-

R

-

Y

-

H

-

L

L

H

F

L

I

V

V

R

G

T

E

H

H

I

F

Q

Q

D

R

D

V

P

-

H

H

N

N

R

T

T

T

R

R

F

F

A

Y

V

I

I

A

G

S

R

R

Y

R

E

P

T

A

E

T

-

L

-

P

P

P

S

G

G

P

S

G

D

F

Q

M

T

T

S

T

M

L

I

L

L

F

S

R

V

V

P

N

N

N

K
x
Q

A

P

G

G

A

A

V
x
L

Y

Y

Q

K

L

A

L

L

A

G

P

G

L

L

A

A

D

T

N

A

G

G

V

V

S
x
N

M
|
M

C
x
T

R
|
R

F

L

E

E

S

S

R

Y

P

M

A

L

R

E

S

G

G

S

A

F

W

S

E

A

Y
x
T

Y

Q

F
|
F

Y

L

V

M

D

D

V

V

E

E

G

G

H

H

Q

P

H

E

D

A

A

P

S

P

V

L

A

A

R

R

A

A

L

L

E

D

E

E

L

L

R

S

R

F

N

F

A

S

A

E

Y

Q

F

Q

K

E

V

I

L

L

G

G

S

V

Y

Y

P

P

S

A

S

S

binding phenylalanine: Q117 (≠ K307), L121 (≠ V311), N134 (≠ S324), M135 (= M325), T136 (≠ C326), R137 (= R327), T150 (≠ Y340), F152 (= F342)

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
35% identity, 21% coverage: 31:108/379 of query aligns to 2:72/345 of 3nvtA

query
sites
3nvtA

L

L

R

R

E

T

Q

Q

I

V

D

D

T

Q

L

L

D

N

R

I

E

D

L

L

A

E

M

L

L

I

N

S

R

K

R

R

A

A

Q

N

L

L

A

V

L

Q

D

E

V

I

G

G

E

K

V

I

K

K

K

G

K

R

Y

-

G

-

A

-

P

-

V

-

F

F

R

D

P

P

E

L

R

R

E

E

L

R

Q

E

V

M

I

L

R

N

K

T

V

I

Q

L

G

A

A

A

N

N

P

E

G

G

P

P

L

F

L

E

D

D

D

S

S

T

V

V

A

Q

A

K

I

L

W

F

R

K

E

E

V

I

M

F

S

K

A

A

C

-

R

-

G

G

L

L

E

E

Sites not aligning to the query:

binding manganese (ii) ion: 114, 284, 310, 321

3tfcA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e in complex with phosphoenolpyruvate (see paper)
35% identity, 21% coverage: 31:108/379 of query aligns to 1:71/343 of 3tfcA

query
sites
3tfcA

L

L

R

R

E

T

Q

Q

I

V

D

D

T

Q

L

L

D

N

R

I

E

D

L

L

A

E

M

L

L

I

N

S

R

K

R

R

A

A

Q

N

L

L

A

V

L

Q

D

E

V

I

G

G

E

K

V

I

K

K

K

L

K

R

Y

-

G

-

A

-

P

-

V

-

F

F

R

D

P

P

E

L

R

R

E

E

L

R

Q

E

V

M

I

L

R

N

K

T

V

I

Q

L

G

A

A

A

N

N

P

E

G

G

P

P

L

F

L

E

D

D

D

S

S

T

V

V

A

Q

A

K

I

L

W

F

R

K

E

E

V

I

M

F

S

K

A

A

C

-

R

-

G

G

L

L

E

E

Sites not aligning to the query:

binding manganese (ii) ion: 113, 283, 309, 320
binding phosphoenolpyruvate: 137, 193, 196, 218, 248, 283

5gmuB Crystal structure of chorismate mutase like domain of bifunctional dahp synthase of bacillus subtilis in complex with chlorogenic acid (see paper)
30% identity, 22% coverage: 27:108/379 of query aligns to 4:83/87 of 5gmuB

query
sites
5gmuB

E

E

L

L

T

E

P

L

L

L

R

R

E

Q

Q

K

I

A

D

D

T

E

L

L

D

N

R

L

E

Q

L

I

L

L

A

K

M

L

L

I

N

N

R

E

R
|
R

A

G

Q

N

L

V

A

V

L

K

D

E

V

I

G

G

E

K

V

A

K

K

K

E

K

A

Y

Q

G

G

A

V

P

N

V
x
R

F
|
F

R
x
D

P

P

E

V

R

R

E
|
E

L

R

Q

T

V

M

I

L

R

N

K

N

V

I

Q

I

G

E

A

N

N

N

P

D

G

G

P

P

L

F

L

E

D

N

D

S

S

T

V

I

A

Q

A

H

I

I

W

F

R

K

E

E

V

I

M
x
F

S
x
K

A

A

C

-

R

-

G

G

L
|
L

E

E

binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: R26 (= R49), R44 (≠ V67), F45 (= F68), D46 (≠ R69), E50 (= E73), F78 (≠ M101), K79 (≠ S102), L82 (= L107)

Sites not aligning to the query:

binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: 85

P39912 Protein AroA(G); EC 2.5.1.54; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see paper)
30% identity, 22% coverage: 27:108/379 of query aligns to 5:84/358 of P39912

query
sites
P39912

E

E

L

L

T

E

P

L

L

L

R

R

E

Q

Q

K

I

A

D

D

T

E

L

L

D

N

R

L

E

Q

L

I

L

L

A

K

M

L

L

I

N

N

R

E

R

R

A

G

Q

N

L

V

A

V

L

K

D

E

V

I

G

G

E

K

V

A

K

K

K

E

K

A

Y

Q

G

G

A

V

P

N

V

R

F

F

R

D

P

P

E

V

R

R

E

E

L

R

Q

T

V

M

I

L

R

N

K

N

V

I

Q

I

G

E

A

N

N

N

P

D

G

G

P

P

L

F

L

E

D

N

D

S

S

T

V

I

A

Q

A

H

I

I

W

F

R

K

E

E

V

I

M

F

S

K

A

A

C

-

R

-

G

G

L

L

E

E

Sites not aligning to the query:

2 modified: Phosphoserine

5j6fA Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
27% identity, 22% coverage: 28:109/379 of query aligns to 4:85/352 of 5j6fA

query
sites
5j6fA

L

L

T

D

P

E

L

L

R

R

E

A

Q

R

I

V

D

D

T

E

L

I

D

N

R

L

E

Q

L

L

A

K

M

L

L

I

N

N

R

E

R
|
R

A

G

Q

R

L

L

A

V

L

Q

D

E

V
x
I

G

G

E

K

V

I

K
|
K

K

E

K

A

Y

Q

G

G

A

T

P

H

V

R

F
x
Y

R
x
D

P

P

E

V

R
|
R

E

E

L

R

Q

K

V
x
M

I

L

R

D

K

L

V

I

Q

S

G

E

A

H

N

N

P

D

G

G

P

P

L

F

L

E

D

T

D

S

S

T

V

L

A

Q

A

H

I

I

W

F

R

K

E

E

V

I

M

F

S

K

A

A

C

A

R

L

G

E

L

L

E

Q

K

E

binding prephenic acid: R25 (= R49), I32 (≠ V56), K36 (= K60), Y44 (≠ F68), D45 (≠ R69), R48 (= R72), M52 (≠ V76)

Sites not aligning to the query:

binding manganese (ii) ion: 124, 294, 320, 331

6al9B Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
27% identity, 22% coverage: 28:109/379 of query aligns to 5:84/91 of 6al9B

query
sites
6al9B

L

L

T

E

P

N

L

L

R

R

E

A

Q

E

I

I

D

D

T

A

L

L

D

D

R

N

E

E

L

L

L

S

A

D

M

L

L

L

N

D

R

K

R

R

A

L

Q

E

L

I

A

A

L

L

D

K

V
x
I

G

A

E

L

V

I

K

K

K

Q

K

E

Y

-

G

-

A

S

P

P

V

I

F

Y

R

C

P

P

E

K

R

R

E

E

L

Q

Q

E

V

I

I

L

R

K

K

R

V

L

Q

S

G

Q

A

R

N

D

P

F

G

K

P

H

L

L

L

N

D

G

D

E

S

I

V

L

A

T

A

G

I

F

W

Y

R

T

E

E

V

V

M

F

S

K

A

I

C

S

R

R

G

K

L

F

E
x
Q

K

E

binding pyruvic acid: I33 (≠ V56), Q83 (≠ E108)

6al9A Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
27% identity, 22% coverage: 28:109/379 of query aligns to 4:83/90 of 6al9A

query
sites
6al9A

L

L

T

E

P

N

L

L

R

R

E

A

Q

E

I

I

D

D

T

A

L

L

D

D

R

N

E

E

L

L

L

S

A

D

M

L

L

L

N

D

R

K

R
|
R

A

L

Q

E

L

I

A

A

L

L

D

K

V

I

G

A

E

L

V

I

K

K

K

Q

K

E

Y

-

G

-

A

S

P

P

V

I

F

Y

R
x
C

P

P

E

K

R
|
R

E
|
E

L

Q

Q

E

V

I

I

L

R

K

K

R

V

L

Q

S

G

Q

A

R

N

D

P

F

G

K

P

H

L

L

L

N

D

G

D

E

S

I

V

L

A

T

A

G

I

F

W

Y

R

T

E

E

V

V

M
x
F

S

K

A

I

C
x
S

R

R

G

K

L

F

E
x
Q

K

E

binding prephenic acid: R25 (= R49), C43 (≠ R69), R46 (= R72), E47 (= E73), F75 (≠ M101), S78 (≠ C104), Q82 (≠ E108)

Query Sequence

>RR42_RS04460 FitnessBrowser__Cup4G11:RR42_RS04460
MTNQDKQGAQGEAQQDNGAAEKALGVELTPLREQIDTLDRELLAMLNRRAQLALDVGEVK
KKYGAPVFRPERELQVIRKVQGANPGPLLDDSVAAIWREVMSACRGLEKPLEVAFLGPAG
TFSEQALYAHFGHEVSGVPCPSIDEVFRAVEAGTVEYGVVPVENSTEGAVSRTLDLFLQT
SLKISGEIALKVHHNLMASSPDMKGVTVVRAHAQALAQCQHWLTANYPHLERQAVSSNAE
AARMASEDPTVAAIAGESAANRYHLHLVRTHIQDDPHNRTRFAVIGRYETEPSGSDQTSM
ILSVPNKAGAVYQLLAPLADNGVSMCRFESRPARSGAWEYYFYVDVEGHQHDASVARALE
ELRRNAAYFKVLGSYPSSR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory