SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS04535 FitnessBrowser__Cup4G11:RR42_RS04535 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
62% identity, 98% coverage: 5:298/300 of query aligns to 3:292/303 of P16703

query
sites
P16703

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N71 (= N77) binding
S255 (= S261) binding

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
62% identity, 98% coverage: 5:298/300 of query aligns to 3:292/294 of 2bhtA

query
sites
2bhtA

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active site: K41 (= K47), S69 (= S75), Q199 (= Q205), G203 (= G209), S255 (= S261), C280 (= C286)
binding pyridoxal-5'-phosphate: K41 (= K47), N71 (= N77), M173 (= M179), G174 (= G180), T175 (= T181), T176 (= T182), T178 (= T184), G208 (= G214), S255 (= S261), C280 (= C286)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
44% identity, 99% coverage: 3:298/300 of query aligns to 5:306/310 of 5xoqA

query
sites
5xoqA

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binding : T72 (= T74), S73 (= S75), G74 (= G76), T76 (= T78), M123 (= M125), Q144 (= Q146), R218 (vs. gap), H219 (vs. gap), Q222 (≠ R216), G223 (≠ K217), A226 (≠ E220)

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
46% identity, 98% coverage: 6:298/300 of query aligns to 7:305/306 of 2q3dA

query
sites
2q3dA

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active site: K44 (= K47), S266 (= S261), P293 (≠ C286)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K47), T71 (= T74), S72 (= S75), N74 (= N77), T75 (= T78), Q144 (= Q146), V177 (≠ M179), G178 (= G180), T179 (= T181), G180 (≠ T182), T182 (= T184), G222 (vs. gap), I223 (vs. gap), S266 (= S261), P293 (≠ C286), D294 (= D287)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
46% identity, 98% coverage: 6:298/300 of query aligns to 7:305/310 of P9WP55

query
sites
P9WP55

I

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L

L

V

V

Q

R

L

L

Q

R

R

R

I

V

P

T

G

D

G

G

A

A

G

V

A

A

P

D

R

-

G

-

N

-

V

-

I

I

L

V

G

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

V

V

S

A

M

M

I

L

A

Q

R

A

A

A

E

E

S

Q

R

A

G

G

R

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

R

R

G

G

Y

Y

K

R

M

C

V

V

L

L

I

T

M

M

P

P

E

E

D

T

L

M

S

S

M

L

E

E

R

R

Q

M

S

L

M

L

A

R

A

A

Y

Y

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

P

V

G

K

A

G

D

G

G

M

M

E

S

Y

G

A

A

R

I

D

A

L

K

A

A

D

E

S

E

M

L

E

A

R

K

-

T

D

D

G

Q

R

R

G

Y

V

F

I

V

L

P

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

L

A

A

I

H

H

Y

R

E

V

T

T

G

A

P

E

E

E

I

V

W

W

L

R

D

D

T

T

E

D

G

G

R

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

A

P

G

S

I

A

Q

R

I

F

V

V

G

A

A

V

Q

E

P

P

A

A

E

A

G

S

S

P

R

V

I

L

P

S

G

G

I

G

R

Q

K

K

W

G

P

P

E

H

-

P

-

I

-

Q

-

G

-

I

-

G

-

A

A

G

Y

F

M

V

P

P

K

P

I

V

Y

L

D

D

P

Q

K

D

F

L

I

V

D

D

R

E

T

I

E

I

P

T

V

V

S

G

Q

N

G

E

D

D

A

A

E

L

H

N

M

V

A

A

R

R

M

L

A

A

R

R

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

L

T

C

V

V

A

A

A

L

L

R

Q

I

V

A

A

E

R

V

P

E

E

N

N

A

A

-

G

-

K

T

L

I

I

V

V

F

V

V

V

V

L

C

P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

K44 (= K47) modified: N6-(pyridoxal phosphate)lysine
N74 (= N77) binding
GTGGT 178:182 (≠ GTTGT 180:184) binding
S266 (= S261) binding

3bm5A Crystal structure of o-acetyl-serine sulfhydrylase from entamoeba histolytica in complex with cysteine (see paper)
43% identity, 99% coverage: 3:298/300 of query aligns to 13:318/338 of 3bm5A

query
sites
3bm5A

Y

Y

K

H

T

N

I

I

E

L

D

E

T

T

I

I

G

G

N

G

T

T

P

P

L

L

V

V

Q

E

L

L

Q

H

R

G

I

V

P

T

G

E

G

H

A

P

G

R

A

I

P

K

R

K

G

G

N

T

V

R

I

I

L

L

G

V

K

K

L

L

E

E

G

Y

N

F

N

N

P

P

A

M

G

S

S

S

V

V

K
|
K

D

D

R

R

P

V

A

G

V

F

S

N

M

I

I

V

A

Y

R

Q

A

A

E

I

S

K

R

D

G

G

R

R

I

L

K

K

P

P

G

G

D

M

T

E

L

I

I

I

E

E

A

S

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

C

M

Q

A

A

A

G

A

A

I

V

R

F

G

G

Y

Y

K

R

M

V

V

N

L

I

I

A

M

M

P

P

E

S

D

T

L

M

S

S

M

V

E

E

R

R

R

Q

Q

M

S

I

M

M

A

K

A

A

Y

F

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

E

V

G

K

K

G

K

G

G

M

M

E

P

Y

G

A

A

R

I

D

E

L

E

A

V

D

N

S

K

M

M

-

I

-

K

E

E

R

N

D

P

G

G

R

K

G

Y

V

F

I

V

L

A

D

N

Q

Q

F

F

A

G

N

N

P

P

D

D

N

N

P

T

L

A

A

A

H

H

Y

H

E

Y

T

T

T

A

G

N

P

-

E

E

I

I

W

W

L

E

D

D

T

T

E

D

G

G

R

E

I

V

T

D

H

I

F

V

V

V

S

S

A

A

M

V

G
|
G

T
|
T

T
x
S

G

G

T
|
T

I

V

T

I

G

G

V

V

S

A

R

E

Y

K

L

L

K

K

E

E

Q

K

N

K

A

K

G

G

I

I

Q

K

I

I

V

I

G

A

A

V

Q

E

P

P

A

E

E

E

G

S

S

A

R

V

I

L

P

E

G

G

I

K

R

A

K

K

W

G

P

P

E

H

-

G

-

I

-

Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

D

I

I

Y

Y

D

K

P

K

K

E

F

F

I

V

D

D

R

E

T

I

E

I

P

P

V

I

S

K

Q

T

G

Q

D

D

A

A

E

W

H

K

M

M

A

A

R

R

R

A

M

V

A

V

R

K

E

Y

E

D

G

G

I

I

F

M

C

C

G

G

I

M

S
|
S

A

S

A

G

G

A

A

A

L

I

C

L

V

A

A

G

L

L

R

K

I

E

A

A

E

E

E

K

V

P

E

E

N

N

-

E

-

G

A

K

T

T

I

I

V

V

F

I

V

I

V

V

C
x
P

D

S

R

C

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

D

V

L

F

Y

active site: K57 (= K47), S279 (= S261)
binding pyridoxal-5'-phosphate: K57 (= K47), N87 (= N77), G191 (= G180), T192 (= T181), S193 (≠ T182), T195 (= T184), G235 (vs. gap), S279 (= S261), P306 (≠ C286)

4jblB Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with methionine (see paper)
43% identity, 99% coverage: 3:298/300 of query aligns to 13:318/335 of 4jblB

query
sites
4jblB

Y

Y

K

H

T

N

I

I

E

L

D

E

T

T

I

I

G

G

N

G

T

T

P

P

L

L

V

V

Q

E

L

L

Q

H

R

G

I

V

P

T

G

E

G

H

A

P

G

R

A

I

P

K

R

K

G

G

N

T

V

R

I

I

L

L

G

V

K

K

L

L

E

E

G

Y

N

F

N

N

P

P

A

M

G

S

S

S

V

V

K
|
K

D

D

R

R

P

V

A

G

V

F

S

N

M

I

I

V

A

Y

R

Q

A

A

E

I

S

K

R

D

G

G

R

R

I

L

K

K

P

P

G

G

D

M

T

E

L

I

I

I

E

E

A

S

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

C

M

Q

A

A

A

G

A

A

I

V

R

F

G

G

Y

Y

K

R

M

V

V

N

L

I

I

A

M

M

P

P

E

S

D

T

L

M

S

S

M

V

E

E

R

R

R

Q

Q

M

S

I

M

M

A

K

A

A

Y

F

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

E

V

G

K

K

G

K

G

G

M
|
M

E

P

Y

G

A

A

R

I

D

E

L

E

A

V

D

N

S

K

M

M

-

I

-

K

E

E

R

N

D

P

G

G

R

K

G

Y

V

F

I

V

L

A

D

N

Q
|
Q

F
|
F

A

G

N

N

P

P

D

D

N

N

P

T

L

A

A

A

H

H

Y

H

E

Y

T

T

T

A

G

N

P

-

E

E

I

I

W

W

L

E

D

D

T

T

E

D

G

G

R

E

I

V

T

D

H

I

F

V

V

V

S

S

A

A

M

V

G
|
G

T

T

T

S

G

G

T

T

I

V

T

I

G

G

V

V

S

A

R

E

Y

K

L

L

K

K

E

E

Q

K

N

K

A

K

G

G

I

I

Q

K

I

I

V

I

G

A

A

V

Q

E

P

P

A

E

E

E

G

S

S

A

R

V

I

L

P

E

G

G

I

K

R

A

K

K

W

G

P

P

E

H

-

G

-

I

-

Q

-
x
G

-
x
I

-

G

-
x
A

A

G

Y

F

M

I

P

P

K

D

I

I

Y

Y

D

K

P

K

K

E

F

F

I

V

D

D

R

E

T

I

E

I

P

P

V

I

S

K

Q

T

G

Q

D

D

A

A

E

W

H

K

M

M

A

A

R

R

R

A

M

V

A

V

R

K

E

Y

E

D

G

G

I

I

F

M

C

C

G

G

I

M

S
|
S

A

S

A

G

G

A

A

A

L

I

C

L

V

A

A

G

L

L

R

K

I

E

A

A

E

E

E

K

V

P

E

E

N

N

-

E

-

G

A

K

T

T

I

I

V

V

F

I

V

I

V

V

C

P

D

S

R

C

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

D

V

L

F

Y

active site: K57 (= K47), S279 (= S261)
binding methionine: K57 (= K47), T84 (= T74), S85 (= S75), N87 (= N77), T88 (= T78), M135 (= M125), Q158 (= Q146), F159 (= F147), G191 (= G180), G235 (vs. gap), I236 (vs. gap), A238 (vs. gap)

3bm5B Crystal structure of o-acetyl-serine sulfhydrylase from entamoeba histolytica in complex with cysteine (see paper)
43% identity, 99% coverage: 3:298/300 of query aligns to 13:318/335 of 3bm5B

query
sites
3bm5B

Y

Y

K

H

T

N

I

I

E

L

D

E

T

T

I

I

G

G

N

G

T

T

P

P

L

L

V

V

Q

E

L

L

Q

H

R

G

I

V

P

T

G

E

G

H

A

P

G

R

A

I

P

K

R

K

G

G

N

T

V

R

I

I

L

L

G

V

K

K

L

L

E

E

G

Y

N

F

N

N

P

P

A

M

G

S

S

S

V

V

K
|
K

D

D

R

R

P

V

A

G

V

F

S

N

M

I

I

V

A

Y

R

Q

A

A

E

I

S

K

R

D

G

G

R

R

I

L

K

K

P

P

G

G

D

M

T

E

L

I

I

I

E

E

A

S

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

C

M

Q

A

A

A

G

A

A

I

V

R

F

G

G

Y

Y

K

R

M

V

V

N

L

I

I

A

M

M

P

P

E

S

D

T

L

M

S

S

M

V

E

E

R

R

R

Q

Q

M

S

I

M

M

A

K

A

A

Y

F

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

E

V

G

K

K

G

K

G

G

M

M

E

P

Y

G

A

A

R

I

D

E

L

E

A

V

D

N

S

K

M

M

-

I

-

K

E

E

R

N

D

P

G

G

R

K

G

Y

V

F

I

V

L

A

D

N

Q

Q

F

F

A

G

N

N

P

P

D

D

N

N

P

T

L

A

A

A

H

H

Y

H

E

Y

T

T

T

A

G

N

P

-

E

E

I

I

W

W

L

E

D

D

T

T

E

D

G

G

R

E

I

V

T

D

H

I

F

V

V

V

S

S

A

A

M

V

G
|
G

T
|
T

T
x
S

G

G

T
|
T

I

V

T

I

G

G

V

V

S

A

R

E

Y

K

L

L

K

K

E

E

Q

K

N

K

A

K

G

G

I

I

Q

K

I

I

V

I

G

A

A

V

Q

E

P

P

A

E

E

E

G

S

S

A

R

V

I

L

P

E

G

G

I

K

R

A

K

K

W

G

P

P

E

H

-

G

-

I

-

Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

D

I

I

Y

Y

D

K

P

K

K

E

F

F

I

V

D

D

R

E

T

I

E

I

P

P

V

I

S

K

Q

T

G

Q

D

D

A

A

E

W

H

K

M

M

A

A

R

R

R

A

M

V

A

V

R

K

E

Y

E

D

G

G

I

I

F

M

C

C

G

G

I

M

S
|
S

A

S

A

G

G

A

A

A

L

I

C

L

V

A

A

G

L

L

R

K

I

E

A

A

E

E

E

K

V

P

E

E

N

N

-

E

-

G

A

K

T

T

I

I

V

V

F

I

V

I

V

V

C
x
P

D

S

R

C

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

D

V

L

F

Y

active site: K57 (= K47), S279 (= S261)
binding cysteine: K57 (= K47), T84 (= T74), S85 (= S75), T88 (= T78), G235 (vs. gap)
binding pyridoxal-5'-phosphate: K57 (= K47), N87 (= N77), G191 (= G180), T192 (= T181), S193 (≠ T182), T195 (= T184), G235 (vs. gap), S279 (= S261), P306 (≠ C286)

4jbnA Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with serine acetyl transferase derived tetrapeptide, spsi (see paper)
43% identity, 99% coverage: 3:298/300 of query aligns to 13:318/336 of 4jbnA

query
sites
4jbnA

Y

Y

K

H

T

N

I

I

E

L

D

E

T

T

I

I

G

G

N

G

T

T

P

P

L

L

V

V

Q

E

L

L

Q

H

R

G

I

V

P

T

G

E

G

H

A

P

G

R

A

I

P

K

R

K

G

G

N

T

V

R

I

I

L

L

G

V

K

K

L

L

E

E

G

Y

N

F

N

N

P

P

A

M

G

S

S

S

V

V

K
|
K

D

D

R

R

P

V

A

G

V

F

S

N

M

I

I

V

A

Y

R

Q

A

A

E

I

S

K

R

D

G

G

R

R

I

L

K

K

P

P

G

G

D

M

T

E

L

I

I

I

E

E

A

S

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

C

M

Q

A

A

A

G

A

A

I

V

R

F

G

G

Y

Y

K

R

M

V

V

N

L

I

I

A

M

M

P

P

E

S

D

T

L

M

S

S

M

V

E

E

R

R

R

Q

Q

M

S

I

M

M

A

K

A

A

Y

F

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

E

V

G

K

K

G

K

G

G

M

M

E

P

Y

G

A

A

R

I

D

E

L

E

A

V

D

N

S

K

M

M

-

I

-

K

E

E

R

N

D

P

G

G

R

K

G

Y

V

F

I

V

L

A

D

N

Q
|
Q

F

F

A

G

N

N

P

P

D

D

N

N

P

T

L

A

A

A

H

H

Y

H

E

Y

T

T

T

A

G

N

P

-

E

E

I

I

W

W

L

E

D

D

T

T

E

D

G

G

R

E

I

V

T

D

H

I

F

V

V

V

S

S

A

A

M

V

G
|
G

T

T

T

S

G

G

T

T

I

V

T

I

G

G

V

V

S

A

R

E

Y

K

L

L

K

K

E

E

Q

K

N

K

A

K

G

G

I

I

Q

K

I

I

V

I

G

A

A

V

Q

E

P

P

A

E

E

E

G

S

S

A

R

V

I

L

P

E

G

G

I

K

R

A

K

K

W

G

P

P

E

H

-

G

-

I

-

Q

-

G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

D

I

I

Y

Y

D

K

P

K

K

E

F

F

I

V

D

D

R

E

T

I

E

I

P

P

V

I

S

K

Q

T

G

Q

D

D

A

A

E

W

H

K

M

M

A

A

R

R

R

A

M

V

A

V

R

K

E

Y

E

D

G

G

I

I

F

M

C

C

G

G

I

M

S
|
S

A

S

A

G

G

A

A

A

L

I

C

L

V

A

A

G

L

L

R

K

I

E

A

A

E

E

E

K

V

P

E

E

N

N

-

E

-

G

A

K

T

T

I

I

V

V

F

I

V

I

V

V

C

P

D

S

R

C

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

D

V

L

F

Y

active site: K57 (= K47), S279 (= S261)
binding : K57 (= K47), T84 (= T74), S85 (= S75), G86 (= G76), T88 (= T78), Q158 (= Q146), G191 (= G180)

4il5A Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with isoleucine (see paper)
43% identity, 99% coverage: 3:298/300 of query aligns to 13:318/336 of 4il5A

query
sites
4il5A

Y

Y

K

H

T

N

I

I

E

L

D

E

T

T

I

I

G

G

N

G

T

T

P

P

L

L

V

V

Q

E

L

L

Q

H

R

G

I

V

P

T

G

E

G

H

A

P

G

R

A

I

P

K

R

K

G

G

N

T

V

R

I

I

L

L

G

V

K

K

L

L

E

E

G

Y

N

F

N

N

P

P

A

M

G

S

S

S

V

V

K
|
K

D

D

R

R

P

V

A

G

V

F

S

N

M

I

I

V

A

Y

R

Q

A

A

E

I

S

K

R

D

G

G

R

R

I

L

K

K

P

P

G

G

D

M

T

E

L

I

I

I

E

E

A

S

T

T

S

S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

C

M

Q

A

A

A

G

A

A

I

V

R

F

G

G

Y

Y

K

R

M

V

V

N

L

I

I

A

M

M

P

P

E

S

D

T

L

M

S

S

M

V

E

E

R

R

R

Q

Q

M

S

I

M

M

A

K

A

A

Y

F

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

E

V

G

K

K

G

K

G

G

M

M

E

P

Y

G

A

A

R

I

D

E

L

E

A

V

D

N

S

K

M

M

-

I

-

K

E

E

R

N

D

P

G

G

R

K

G

Y

V

F

I

V

L

A

D

N

Q
|
Q

F
|
F

A

G

N

N

P

P

D

D

N

N

P

T

L

A

A

A

H

H

Y

H

E

Y

T

T

T

A

G

N

P

-

E

E

I

I

W

W

L

E

D

D

T

T

E

D

G

G

R

E

I

V

T

D

H

I

F

V

V

V

S

S

A

A

M

V

G
|
G

T

T

T

S

G

G

T

T

I

V

T

I

G

G

V

V

S

A

R

E

Y

K

L

L

K

K

E

E

Q

K

N

K

A

K

G

G

I

I

Q

K

I

I

V

I

G

A

A

V

Q

E

P

P

A

E

E

E

G

S

S

A

R

V

I

L

P

E

G

G

I

K

R

A

K

K

W

G

P

P

E

H

-

G

-

I

-

Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

D

I

I

Y

Y

D

K

P

K

K

E

F

F

I

V

D

D

R

E

T

I

E

I

P

P

V

I

S

K

Q

T

G

Q

D

D

A

A

E

W

H

K

M

M

A

A

R

R

R

A

M

V

A

V

R

K

E

Y

E

D

G

G

I

I

F

M

C

C

G

G

I

M

S
|
S

A

S

A

G

G

A

A

A

L

I

C

L

V

A

A

G

L

L

R

K

I

E

A

A

E

E

E

K

V

P

E

E

N

N

-

E

-

G

A

K

T

T

I

I

V

V

F

I

V

I

V

V

C

P

D

S

R

C

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

D

V

L

F

Y

active site: K57 (= K47), S279 (= S261)
binding isoleucine: K57 (= K47), G86 (= G76), T88 (= T78), Q158 (= Q146), F159 (= F147), G191 (= G180), G235 (vs. gap)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
43% identity, 99% coverage: 4:300/300 of query aligns to 7:310/322 of P47998

query
sites
P47998

K

K

T

D

I

V

E

T

D

E

T

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

Y

L

L

Q

N

R

N

I

V

P

A

G

E

G

G

A

C

G

V

A

G

P

R

R

-

G

-

N

-

V

-

I

V

L

A

G

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

V

F

S

S

M

M

I

I

A

S

R

D

A

A

E

E

S

K

R

K

G

G

R

L

I

I

K

K

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

R

K

G

G

Y

Y

K

K

M

L

V

I

L

I

I

T

M

M

P

P

E

A

D

S

L

M

S

S

M

T

E

E

R

R

Q

I

S

I

M

L

A

L

A

A

Y

F

G

G

A

V

E

E

I

L

I

V

L

L

T

T

P

D

V

P

K

A

G

K

G

G

M

M

E

K

Y

G

A

A

R

I

D

A

L

K

A

A

D

E

S

E

-

I

M

L

E

A

R

K

D

T

G

P

R

N

G

G

V

Y

I

M

L

L

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

L

K

A

I

H
|
H

Y

Y

E

E

T

T

G
|
G

P

P

E

E

I

I

W

W

L

K

D

G

T

T

E

G

G

G

R

K

I

I

T

D

H

G

F

F

V

V

S

S

A

G

M

I

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

A

A

G

N

I

V

Q

K

I

L

V

Y

G

G

A

V

Q

E

P

P

A

V

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R
x
K

K

P

W

G

P

P

E
x
H

-
x
K

-

I

-

Q

-

G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

S

I

V

Y

L

D

N

P

V

K

D

F

L

I

I

D

D

R

E

T

V

E

V

P

Q

V

V

S

S

Q

S

G

D

D

E

A

S

E

I

H

D

M

M

A

A

R

R

R

Q

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

L

A

C

A

V

A

A

A

L

I

R

K

I

L

A

A

E

Q

E

R

V

P

E

E

N

N

A

A

T

G

I

K

V

L

F

F

V

V

V

A

C

I

-

F

-

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

K46 (= K47) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T74) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S75) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N77) binding ; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T78) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q146) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H156) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G161) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTTGT 180:184) binding
T182 (= T181) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T184) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ R216) mutation to A: Impaired interaction with SAT1.
H221 (≠ E220) mutation to A: Impaired interaction with SAT1.
K222 (vs. gap) mutation to A: Impaired interaction with SAT1.
S269 (= S261) binding ; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
43% identity, 99% coverage: 4:300/300 of query aligns to 5:308/320 of 2isqA

query
sites
2isqA

K

K

T

D

I

V

E

T

D

E

T

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

Y

L

L

Q

N

R

N

I

V

P

A

G

E

G

G

A

C

G

V

A

G

P

R

R

-

G

-

N

-

V

-

I

V

L

A

G

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

V

F

S

S

M

M

I

I

A

S

R

D

A

A

E

E

S

K

R

K

G

G

R

L

I

I

K

K

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

R

K

G

G

Y

Y

K

K

M

L

V

I

L

I

I

T

M

M

P

P

E

A

D

S

L

M

S

S

M

T

E

E

R

R

Q

I

S

I

M

L

A

L

A

A

Y

F

G

G

A

V

E

E

I

L

I

V

L

L

T

T

P

D

V

P

K

A

G

K

G
|
G

M
|
M

E
x
K

Y

G

A

A

R

I

D

A

L

K

A

A

D

E

S

E

-

I

M

L

E

A

R

K

D

T

G

P

R

N

G

G

V

Y

I

M

L

L

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

L

K

A

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

W

W

L

K

D

G

T

T

E

G

G

G

R

K

I

I

T

D

H

G

F

F

V

V

S

S

A
x
G

M

I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

A

A

G

N

I

V

Q

K

I

L

V

Y

G

G

A

V

Q

E

P

P

A

V

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R

K

K

P

W
x
G

P
|
P

E
x
H

-

K

-

I

-
x
Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

S

I

V

Y

L

D

N

P

V

K

D

F

L

I

I

D

D

R

E

T

V

E

V

P

Q

V

V

S

S

Q

S

G

D

D

E

A

S

E

I

H

D

M

M

A

A

R

R

R

Q

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

L

A

C

A

V

A

A

A

L

I

R

K

I

L

A

A

E

Q

E

R

V

P

E

E

N

N

A

A

T

G

I

K

V

L

F

F

V

V

V

A

C

I

-

F

-
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

active site: K44 (= K47), S267 (= S261)
binding pyridoxal-5'-phosphate: K44 (= K47), N75 (= N77), G177 (≠ A178), G179 (= G180), T180 (= T181), G181 (≠ T182), T183 (= T184), G223 (vs. gap), S267 (= S261), P294 (vs. gap)
binding : T72 (= T74), S73 (= S75), G74 (= G76), T76 (= T78), G122 (= G124), M123 (= M125), K124 (≠ E126), G217 (≠ W218), P218 (= P219), H219 (≠ E220), Q222 (vs. gap), G223 (vs. gap)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
45% identity, 96% coverage: 6:293/300 of query aligns to 7:300/300 of 3zeiA

query
sites
3zeiA

I

I

E

T

D

Q

T

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

Q

R

L

L

Q

R

R

R

I

V

P

T

G

D

G

G

A

A

G

V

A

A

P

D

R

-

G

-

N

-

V

-

I

I

L

V

G

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

V

V

S

A

M

M

I

L

A

Q

R

A

A

A

E

E

S

Q

R

A

G

G

R

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

R

R

G

G

Y

Y

K

R

M

C

V

V

L

L

I

T

M

M

P

P

E

E

D

T

L

M

S

S

M

L

E

E

R

R

Q

M

S

L

M

L

A

R

A

A

Y

Y

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

P

V

G

K

A

G

D

G

G

M

M

E

S

Y

G

A

A

R
x
I

D

A

L

K

A

A

D

E

S

E

M

L

E

A

R

K

-

T

D

D

G

Q

R

R

G

Y

V

F

I

V

L

P

D

Q

Q
|
Q

F
|
F

A

E

N

N

P

P

D

A

N

N

P

P

L

A

A

I

H

H

Y

R

E

V

T

T

G

A

P

E

E

E

I

V

W

W

L

R

D

D

T

T

E

D

G

G

R

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

A

P

G

S

I

A

Q

R

I

F

V

V

G

A

A

V

Q

E

P

P

A

A

E

A

G

S

S

P

R

V

I

L

P

S

G

G

I

G

R

Q

K
|
K

W

G

P

P

E

H

-

P

-

I

-

Q

-
x
G

-

I

-

G

-
x
A

A

G

Y
x
F

M

V

P

P

K

P

I

V

Y

L

D

D

P

Q

K

D

F

L

I

V

D

D

R

E

T

I

E

I

P

T

V

V

S

G

Q

N

G

E

D

D

A

A

E

L

H

N

M

V

A

A

R

R

M

L

A

A

R

R

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

L

T

C

V

V

A

A

A

L

L

R

Q

I

V

A

A

E

R

V

P

E

E

N

N

A

A

-

G

-

K

T

L

I

I

V

V

F

V

V

V

V

L

C
x
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K47), S266 (= S261), P293 (≠ C286)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T74), S72 (= S75), I126 (≠ R129), Q144 (= Q146), F145 (= F147), K215 (= K217), G222 (vs. gap), A225 (vs. gap), F227 (≠ Y222)
binding pyridoxal-5'-phosphate: K44 (= K47), N74 (= N77), V177 (≠ M179), G178 (= G180), T179 (= T181), G180 (≠ T182), T182 (= T184), G222 (vs. gap), S266 (= S261), P293 (≠ C286), D294 (= D287)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
45% identity, 96% coverage: 6:293/300 of query aligns to 7:300/300 of 2q3cA

query
sites
2q3cA

I

I

E

T

D

Q

T

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

Q

R

L

L

Q

R

R

R

I

V

P

T

G

D

G

G

A

A

G

V

A

A

P

D

R

-

G

-

N

-

V

-

I

I

L

V

G

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

V

V

S

A

M

M

I

L

A

Q

R

A

A

A

E

E

S

Q

R

A

G

G

R

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

R

R

G

G

Y

Y

K

R

M

C

V

V

L

L

I

T

M

M

P

P

E

E

D

T

L

M

S

S

M

L

E

E

R

R

Q

M

S

L

M

L

A

R

A

A

Y

Y

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

P

V

G

K

A

G

D

G

G

M
|
M

E

S

Y

G

A

A

R

I

D

A

L

K

A

A

D

E

S

E

M

L

E

A

R

K

-

T

D

D

G

Q

R

R

G

Y

V

F

I

V

L

P

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

L

A

A

I

H

H

Y

R

E

V

T

T

G

A

P

E

E

E

I

V

W

W

L

R

D

D

T

T

E

D

G

G

R

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M

V

G

G

T

T

T

G

G

G

T

T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

A

P

G

S

I

A

Q

R

I

F

V

V

G

A

A

V

Q

E

P

P

A

A

E

A

G

S

S

P

R

V

I

L

P

S

G

G

I

G

R

Q

K
|
K

W

G

P

P

E

H

-

P

-

I

-

Q

-
x
G

-

I

-

G

-
x
A

A

G

Y

F

M

V

P

P

K

P

I

V

Y

L

D

D

P

Q

K

D

F

L

I

V

D

D

R

E

T

I

E

I

P

T

V

V

S

G

Q

N

G

E

D

D

A

A

E

L

H

N

M

V

A

A

R

R

M

L

A

A

R

R

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

L

T

C

V

V

A

A

A

L

L

R

Q

I

V

A

A

E

R

V

P

E

E

N

N

A

A

-

G

-

K

T

L

I

I

V

V

F

V

V

V

V

L

C
x
P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K47), S266 (= S261), P293 (≠ C286)
binding : T71 (= T74), S72 (= S75), G73 (= G76), T75 (= T78), M122 (= M125), Q144 (= Q146), K215 (= K217), G222 (vs. gap), A225 (vs. gap)

P9WP53 O-phosphoserine sulfhydrylase; OPS sulfhydrylase; CysO-thiocarboxylate-dependent cysteine synthase; Cysteine synthase B; CSase B; O-phosphoserine-specific cysteine synthase; [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase; EC 2.5.1.113 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
42% identity, 99% coverage: 3:298/300 of query aligns to 4:306/323 of P9WP53

query
sites
P9WP53

Y

Y

K

D

T

S

I

L

E

L

D

Q

T

A

I

L

G

G

N

N

T

T

P

P

L

L

V

V

Q

G

L

L

Q

Q

R

R

I

L

P

-

G

-

A

-

G

-

A

S

P

P

R

R

G

W

N

D

-

D

-

G

-

R

-

D

-

G

-

P

-

H

-

V

V

R

I

L

L

W

G

A

K

K

L

L

E

E

G

D

N

R

N

N

P

P

A

T

G

G

S

S

V

I

K

K

D

D

R

R

P

P

A

A

V

V

S

R

M

M

I

I

A

E

R

Q

A

A

E

E

S

A

R

D

G

G

R

L

I

L

K

R

P

P

G

G

D

A

T

T

L

I

I

L

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

S

L

L

A

A

M

M

A

A

A

R

I

L

R

K

G

G

Y

Y

K

R

M

L

V

I

L

C

I

V

M

M

P

P

E

E

D

N

L

T

S

S

M

V

E

E

R

R

Q

Q

S

L

M

L

A

E

A

L

Y

Y

G

G

A

A

E

Q

I

I

L

F

T

S

P

A

V

A

K

E

G

G

M

S

E

N

Y

T

A

A

R

V

D

A

L

T

A

A

D

K

S

E

M

L

-

A

E

A

R

T

D

N

G

P

R

S

G

W

V

V

I

M

L

L

D

Y

Q

Q

F

Y

A

G

N

N

P

P

D

A

N

N

P

T

L

D

A

S

H

H

Y

Y

E

C

T

G

T

T

G

G

P

P

E

E

I

L

W

L

L

A

D

D

T

L

E

P

G

-

R

E

I

I

T

T

H

H

F

F

V

V

S

A

A

G

M

L

G

G

T

T

G

G

T

T

I

L

T

M

G

G

V

T

S

G

R

R

Y

F

L

L

K

R

E

E

Q

H

N

V

A

A

G

N

I

V

Q

K

I

I

V

V

G

A

A

A

Q

E

P

P

A

R

E

Y

G

G

S

E

R

G

I

V

P

Y

G

A

I

L

R
|
R

K

N

W

M

P

D

E

E

A

G

Y

F

M

V

P

P

K

E

I

L

Y

Y

D

D

P

P

K

E

F

I

I

L

D

T

R

A

T

R

E

Y

P

S

V

V

S

G

Q

A

G

V

D

D

A

A

E

V

H

R

M

R

A

T

R

R

R

E

M

L

A

V

R

H

E

T

E

E

G

G

I

I

F

F

C

A

G

G

I

I

S
|
S

A

T

A

G

G

A

A

V

L

L

-

H

-

A

-

L

C

G

V

V

A

G

L

A

R

G

I

A

A

L

E

A

V

G

E

E

N

R

A

A

T

D

I

I

V

A

F

L

V

V

C

A

D

D

R

A

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

G

V

A

F

Y

N81 (= N77) binding
R220 (= R216) mutation to A: 700-fold decrease in the rate of the first half-reaction using OPS. Affects neither the rate of the first half-reaction using OAS nor the rate of the second half-reaction using sulfide or CysO-COSH.
S265 (= S261) binding

Sites not aligning to the query:

319:323 mutation Missing: Decreased lifetime of the alpha-aminoacrylate reaction intermediate, increased susceptibility to oxidation by oxidative agents such as hydrogen peroxide, and partial loss of selectivity towards CysO-COSH as sulfur donor.

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
43% identity, 99% coverage: 4:300/300 of query aligns to 5:308/320 of 1z7yA

query
sites
1z7yA

K

K

T

D

I

V

E

T

D

E

T

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

Y

L

L

Q

N

R

N

I

V

P

A

G

E

G

G

A

C

G

V

A

G

P

R

R

-

G

-

N

-

V

-

I

V

L

A

G

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
x
A

D

D

R

R

P

I

A

G

V

F

S

S

M

M

I

I

A

S

R

D

A

A

E

E

S

K

R

K

G

G

R

L

I

I

K

K

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

R

K

G

G

Y

Y

K

K

M

L

V

I

L

I

I

T

M

M

P

P

E

A

D

S

L

M

S

S

M

T

E

E

R

R

Q

I

S

I

M

L

A

L

A

A

Y

F

G

G

A

V

E

E

I

L

I

V

L

L

T

T

P

D

V

P

K

A

G

K

G

G

M

M

E

K

Y

G

A

A

R

I

D

A

L

K

A

A

D

E

S

E

-

I

M

L

E

A

R

K

D

T

G

P

R

N

G

G

V

Y

I

M

L

L

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

L

K

A

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

W

W

L

K

D

G

T

T

E

G

G

G

R

K

I

I

T

D

H

G

F

F

V

V

S

S

A

G

M
x
I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

A

A

G

N

I

V

Q

K

I

L

V

Y

G

G

A

V

Q

E

P

P

A

V

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R

K

K

P

W

G

P

P

E

H

-

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

S

I

V

Y

L

D

N

P

V

K

D

F

L

I

I

D

D

R

E

T

V

E

V

P

Q

V

V

S

S

Q

S

G

D

D

E

A

S

E

I

H

D

M

M

A

A

R

R

R

Q

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

L

A

C

A

V

A

A

A

L

I

R

K

I

L

A

A

E

Q

E

R

V

P

E

E

N

N

A

A

T

G

I

K

V

L

F

F

V

V

V

A

C

I

-

F

-
x
P

D
x
S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

active site: A44 (≠ K47), S267 (= S261)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G76), N75 (= N77), T76 (= T78), Q145 (= Q146), I178 (≠ M179), G179 (= G180), T180 (= T181), G181 (≠ T182), T183 (= T184), G223 (vs. gap), S267 (= S261), P294 (vs. gap), S295 (≠ D287)

3dwgA Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of mycobacterium tuberculosis (see paper)
42% identity, 99% coverage: 3:298/300 of query aligns to 6:308/325 of 3dwgA

query
sites
3dwgA

Y

Y

K

D

T

S

I

L

E

L

D

Q

T

A

I

L

G

G

N

N

T

T

P

P

L

L

V

V

Q

G

L

L

Q

Q

R

R

I

L

P

-

G

-

A

-

G

-

A

S

P

P

R

R

G

W

N

D

-

D

-

G

-

R

-

D

-

G

-

P

-

H

-

V

V

R

I

L

L

W

G

A

K

K

L

L

E

E

G

D

N

R

N

N

P

P

A

T

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

V

V

S

R

M

M

I

I

A

E

R

Q

A

A

E

E

S

A

R

D

G

G

R

L

I

L

K

R

P

P

G

G

D

A

T

T

L

I

I

L

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

S

L

L

A

A

M

M

A

A

A

R

I

L

R

K

G

G

Y

Y

K

R

M

L

V

I

L

C

I

V

M

M

P

P

E

E

D

N

L

T

S

S

M

V

E

E

R

R

Q

Q

S

L

M

L

A

E

A

L

Y

Y

G

G

A

A

E

Q

I

I

L

F

T

S

P

A

V

A

K

E

G

G

M

S

E

N

Y

T

A

A

R

V

D

A

L

T

A

A

D

K

S

E

M

L

-

A

E

A

R

T

D

N

G

P

R

S

G

W

V

V

I

M

L

L

D

Y

Q

Q

F

Y

A

G

N

N

P

P

D

A

N

N

P

T

L

D

A

S

H

H

Y

Y

E

C

T

G

T

T

G

G

P

P

E

E

I

L

W

L

L

A

D

D

T

L

E

P

G

-

R

E

I

I

T

T

H

H

F

F

V

V

S

A

A

G

M

L

G
|
G

T
|
T

G
|
G

T
|
T

I

L

T

M

G

G

V

T

S

G

R

R

Y

F

L

L

K

R

E

E

Q

H

N

V

A

A

G

N

I

V

Q

K

I

I

V

V

G

A

A

A

Q

E

P

P

A

R

E

Y

G

G

S

E

R

G

I

V

P

Y

G

A

I

L

R

R

K

N

W

M

P

D

E

E

A

G

Y

F

M

V

P

P

K

E

I

L

Y

Y

D

D

P

P

K

E

F

I

I

L

D

T

R

A

T

R

E

Y

P

S

V

V

S

G

Q

A

G

V

D

D

A

A

E

V

H

R

M

R

A

T

R

R

R

E

M

L

A

V

R

H

E

T

E

E

G

G

I

I

F

F

C

A

G

G

I

I

S
|
S

A

T

A

G

G

A

A

V

L

L

-

H

-

A

-

L

C

G

V

V

A

G

L

A

R

G

I

A

A

L

E

A

V

G

E

E

N

R

A

A

T

D

I

I

V

A

F

L

V

V

C
x
A

D
|
D

R

A

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

G

V

A

F

Y

active site: K53 (= K47), S267 (= S261)
binding pyridoxal-5'-phosphate: K53 (= K47), N83 (= N77), G186 (= G180), T187 (= T181), T188 (= T182), G189 (= G183), T190 (= T184), S267 (= S261), A296 (≠ C286), D297 (= D287)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
42% identity, 98% coverage: 4:298/300 of query aligns to 13:314/329 of 3vbeC

query
sites
3vbeC

K

K

T

H

I

V

E

S

D

Q

T

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

Q

Y

L

L

Q

N

R

K

I

V

P

T

G

E

G

G

A

C

G

G

A

A

P

-

R

-

G

-

N

-

V

Y

I

V

L

A

G

V

K

K

L

Q

E

E

G

M

N

M

N

Q

P

P

A

T

G

A

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

V

Y

S

A

M

M

I

I

A

T

R

D

A

A

E

E

S

E

R

K

G

N

R

L

I

I

K

T

P

P

G

G

D

K

T

T

-

T

L

L

I

I

E

E

A

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

A

S

L

M

A

A

M

F

A

M

A

A

I

M

R

K

G

G

Y

Y

K

K

M

M

V

V

L

L

I

T

M

M

P

P

E

S

D

Y

L

T

S

S

M

L

E

E

R

R

Q

V

S

T

M

M

A

R

A

A

Y

F

G

G

A

A

E

E

I

L

I

I

L

L

T

T

-

D

P

P

V

A

K

K

G

G

G

M

M

G

E

G

Y

T

A

V

R

K

D

K

L

A

A

Y

D

E

S

L

M

L

E

E

R

N

D

T

G

P

R

N

G

A

V

H

I

M

L

L

D

Q

Q

Q

F

F

A

S

N

N

P

P

D

A

N

N

P

T

L

Q

A

V

H

H

Y

F

E

E

T

T

G

G

P

P

E

E

I

I

W

W

L

E

D

D

T

T

E

N

G

G

R

Q

I

V

T

D

H

I

F

F

V

V

S
x
M

A

G

M

I

G
|
G

T
x
S

T
x
G

G

G

T
|
T

I

V

T

S

G

G

V

V

S

G

R

Q

Y

Y

L

L

K

K

E

S

Q

K

N

N

A

P

G

N

I

V

Q

K

I

I

V

Y

G

G

A

V

Q
x
E

P

P

A

S

E

E

G
x
S

S

N

R

V

I

L

P

N

G

G

I

G

R

K

K

P

W

G

P

P

E

H

-

H

-

I

-

T

-
x
G

-

N

-

G

-

V

A

G

Y

F

M

K

P

P

K

D

I

I

Y

L

D

D

P

L

K

D

F

V

I

M

D

E

R

K

T

V

E

L

P

E

V

V

S

S

Q

S

G

E

D

D

A

A

E

V

H

N

M

M

A

A

R

R

R

V

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

M

C

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

N

L

T

C

V

V

A

A

A

L

L

R

R

I

L

A

A

E

Q

E

L

V

P

E

E

N

N

-

K

-

G

A

K

T

L

I

I

V

V

F

T

V

V

V

H

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

S

G

V

V

L

F

F

active site: K52 (= K47), S81 (= S75), E212 (≠ Q205), S216 (≠ G209), S275 (= S261), P302 (≠ C286)
binding pyridoxal-5'-phosphate: K52 (= K47), N83 (= N77), M184 (≠ S177), G187 (= G180), S188 (≠ T181), G189 (≠ T182), T191 (= T184), G231 (vs. gap), S275 (= S261), P302 (≠ C286)

7xoyA Cystathionine beta-synthase of mycobacterium tuberculosis in the presence of s-adenosylmethionine and serine. (see paper)
45% identity, 97% coverage: 4:294/300 of query aligns to 3:301/458 of 7xoyA

query
sites
7xoyA

K

Q

T

H

I

I

E

S

D

E

T

L

I

I

G

G

N

G

T

T

P

P

L

L

V

V

Q

R

L

L

Q

N

R

S

I

V

-

V

P

P

G

D

G

G

A

A

G

G

A

-

P

-

R

-

G

-

N

-

V

T

I

V

L

A

G

A

K

K

L

V

E

E

G

Y

N

L

N

N

P

P

A

G

G

G

S

S

V

S

K
|
K

D

D

R

R

P

I

A

A

V

V

S

K

M

M

I

I

A

E

R

A

A

A

E

E

S

A

R

S

G

G

R

Q

I

L

K

K

P

P

G

G

D

G

T

T

L

I

I

V

E

E

A

P

T

T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

L

A

V

A

A

A

Q

I

R

R

R

G

G

Y

Y

K

K

M

C

V

V

L

F

I

V

M

C

P

P

E

D

D

K

L

V

S

S

M

E

E

D

R

K

R

R

Q

N

S

V

M

L

A

I

A

A

Y

Y

G

G

A

A

E

E

I

V

L

V

T

C

P

P

V

T

-

A

-

V

-

P

K

H

G

D

G

P

M

A

E

S

Y

Y

A

-

R

Y

D

S

L

V

A

S

D

D

S

R

M

L

E

V

R

R

D

D

G

I

R

D

G

G

V

A

I

W

L

K

-

P

D

D

Q

Q

F

Y

A

A

N

N

P

P

D

E

N

G

P

P

L

A

A

S

H

H

Y

Y

E

V

T

T

G

G

P

P

E

E

I

I

W

W

L

A

D

D

T

T

E

E

G

G

R

K

I

V

T

T

H

H

F

F

V

V

S

A

A

G

M

I

G

G

T
|
T

T

G

G
|
G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

R

Y

Y

L

L

K

K

E

E

Q

V

N

S

A

G

G

G

-

R

I

V

Q

R

I

I

V

V

G

G

A

A

Q

D

P

P

A

-

E

E

G

G

S

S

R

V

I

Y

P

S

G

G

I

G

R

A

K

G

W

R

P

P

-

Y

-

L

-

V

-

E

-

G

-

V

-

G

E

E

A

D

Y

F

M

W

P

P

K

A

I

A

Y

Y

D

D

P

P

K

S

F

V

I

P

D

D

R

E

T

I

E

I

P

A

V

V

S

S

Q

D

G

S

D

D

A

S

E

F

H

D

M

M

A

T

R

R

M

L

A

A

R

R

E

E

G

A

I

M

F

L

C

V

G

G

I

G

S
|
S

A

C

A

G

G

M

A

A

L

V

C

V

V

A

A

A

L

L

R

K

I

V

A

A

E

E

V

A

-

G

E

P

N

D

A

A

T

L

I

I

V

V

F

V

V

L

C
x
P

D

D

R

G

G

G

D

R

R

G

Y

Y

L

M

S

S

binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: K42 (= K47), S70 (= S75), N72 (= N77), T73 (= T78), T180 (= T181), G182 (= G183), T183 (= T184), S267 (= S261), P293 (≠ C286)

7xnzB Native cystathionine beta-synthase of mycobacterium tuberculosis. (see paper)
45% identity, 97% coverage: 4:294/300 of query aligns to 3:301/458 of 7xnzB

query
sites
7xnzB

K

Q

T

H

I

I

E

S

D

E

T

L

I

I

G

G

N

G

T

T

P

P

L

L

V

V

Q

R

L

L

Q

N

R

S

I

V

-

V

P

P

G

D

G

G

A

A

G

G

A

-

P

-

R

-

G

-

N

-

V

T

I

V

L

A

G

A

K

K

L

V

E

E

G

Y

N

L

N

N

P

P

A

G

G

G

S

S

V

S

K
|
K

D

D

R

R

P

I

A

A

V

V

S

K

M

M

I

I

A

E

R

A

A

A

E

E

S

A

R

S

G

G

R

Q

I

L

K

K

P

P

G

G

D

G

T

T

L

I

I

V

E

E

A

P

T

T

S

S

G

G

N

N

T

T

G

G

I

V

A

G

L

L

A

A

M

L

A

V

A

A

A

Q

I

R

R

R

G

G

Y

Y

K

K

M

C

V

V

L

F

I

V

M

C

P

P

E

D

D

K

L

V

S

S

M

E

E

D

R

K

R

R

Q

N

S

V

M

L

A

I

A

A

Y

Y

G

G

A

A

E

E

I

V

L

V

T

C

P

P

V

T

-

A

-

V

-

P

K

H

G

D

G

P

M

A

E

S

Y

Y

A

-

R

Y

D

S

L

V

A

S

D

D

S

R

M

L

E

V

R

R

D

D

G

I

R

D

G

G

V

A

I

W

L

K

-

P

D

D

Q

Q

F

Y

A

A

N

N

P

P

D

E

N

G

P

P

L

A

A

S

H

H

Y

Y

E

V

T

T

G

G

P

P

E

E

I

I

W

W

L

A

D

D

T

T

E

E

G

G

R

K

I

V

T

T

H

H

F

F

V

V

S

A

A

G

M

I

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

R

Y

Y

L

L

K

K

E

E

Q

V

N

S

A

G

G

G

-

R

I

V

Q

R

I

I

V

V

G

G

A

A

Q

D

P

P

A

-

E

E

G

G

S

S

R

V

I

Y

P

S

G

G

I

G

R

A

K

G

W

R

P

P

-

Y

-

L

-

V

-

E

-

G

-

V

-

G

E

E

A

D

Y

F

M

W

P

P

K

A

I

A

Y

Y

D

D

P

P

K

S

F

V

I

P

D

D

R

E

T

I

E

I

P

A

V

V

S

S

Q

D

G

S

D

D

A

S

E

F

H

D

M

M

A

T

R

R

M

L

A

A

R

R

E

E

G

A

I

M

F

L

C

V

G

G

I

G

S
|
S

A

C

A

G

G

M

A

A

L

V

C

V

V

A

A

A

L

L

R

K

I

V

A

A

E

E

V

A

-

G

E

P

N

D

A

A

T

L

I

I

V

V

F

V

V

L

C
x
P

D
|
D

R

G

G

G

D

R

R

G

Y

Y

L

M

S

S

binding pyridoxal-5'-phosphate: K42 (= K47), G179 (= G180), T180 (= T181), G181 (≠ T182), G182 (= G183), T183 (= T184), S267 (= S261), P293 (≠ C286), D294 (= D287)

Query Sequence

>RR42_RS04535 FitnessBrowser__Cup4G11:RR42_RS04535
MAYKTIEDTIGNTPLVQLQRIPGGAGAPRGNVILGKLEGNNPAGSVKDRPAVSMIARAES
RGRIKPGDTLIEATSGNTGIALAMAAAIRGYKMVLIMPEDLSMERRQSMAAYGAEIILTP
VKGGMEYARDLADSMERDGRGVILDQFANPDNPLAHYETTGPEIWLDTEGRITHFVSAMG
TTGTITGVSRYLKEQNAGIQIVGAQPAEGSRIPGIRKWPEAYMPKIYDPKFIDRTEPVSQ
GDAEHMARRMAREEGIFCGISAAGALCVALRIAEEVENATIVFVVCDRGDRYLSTGVFPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory