SitesBLAST
Comparing RR42_RS04575 FitnessBrowser__Cup4G11:RR42_RS04575 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 11 hits to proteins with known functional sites (download)
P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
61% identity, 99% coverage: 2:310/311 of query aligns to 21:330/333 of P13804
- G116 (= G95) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
- T171 (= T150) to I: decreased protein stability; dbSNP:rs1801591
- R223 (= R202) binding FAD
- S248 (= S228) binding FAD
- R249 (= R229) mutation to A: Loss of electron transfer activity.
- VGQT 263:266 (= VGQT 243:246) binding FAD
- T266 (= T246) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
- SGAIQH 281:286 (= SGAIQH 261:266) binding FAD
- N300 (= N280) binding FAD
- DL 318:319 (= DL 298:299) binding FAD
Sites not aligning to the query:
- 20:204 Domain I
- 205:333 Domain II
2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
61% identity, 99% coverage: 2:310/311 of query aligns to 3:312/315 of 2a1uA
- binding flavin-adenine dinucleotide: G204 (= G201), R205 (= R202), S230 (= S228), R231 (= R229), A232 (= A230), Q244 (= Q242), V245 (= V243), G246 (= G244), T248 (= T246), G261 (= G259), I262 (= I260), S263 (= S261), A265 (= A263), Q267 (= Q265), H268 (= H266), N282 (= N280), K283 (= K281), D300 (= D298), L301 (= L299)
1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
64% identity, 90% coverage: 30:310/311 of query aligns to 29:307/307 of 1efpA
- binding flavin-adenine dinucleotide: G199 (= G201), R200 (= R202), G201 (= G203), S225 (= S228), R226 (= R229), A227 (= A230), Q239 (= Q242), V240 (= V243), G241 (= G244), T243 (= T246), G256 (= G259), I257 (= I260), S258 (= S261), A260 (= A263), Q262 (= Q265), H263 (= H266), N277 (= N280), K278 (= K281), D295 (= D298), L296 (= L299)
6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
38% identity, 92% coverage: 25:310/311 of query aligns to 94:388/393 of 6fahE
- binding flavin-adenine dinucleotide: L180 (≠ Q110), R200 (= R125), M281 (≠ R202), G282 (= G203), R307 (= R229), A308 (= A230), Q320 (= Q242), V321 (= V243), G322 (= G244), Q323 (= Q245), T324 (= T246), G337 (= G259), I338 (= I260), S339 (= S261), Q343 (= Q265), H344 (= H266), N358 (= N280), K359 (= K281), L377 (= L299)
Sites not aligning to the query:
- binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49
5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
41% identity, 80% coverage: 62:310/311 of query aligns to 45:291/292 of 5ow0A
- binding flavin-adenine dinucleotide: G183 (= G201), R184 (= R202), G185 (= G203), S209 (= S228), R210 (= R229), Q223 (= Q242), I224 (≠ V243), G225 (= G244), T227 (= T246), G240 (= G259), V241 (≠ I260), S242 (= S261), A244 (= A263), Q246 (= Q265), H247 (= H266), N261 (= N280), K262 (= K281), D279 (= D298), Y280 (≠ L299)
5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
40% identity, 74% coverage: 81:310/311 of query aligns to 87:324/331 of 5ol2A
- binding calcium ion: D188 (≠ S176)
- binding flavin-adenine dinucleotide: T117 (≠ L111), R136 (= R125), I147 (≠ V136), G216 (= G201), R217 (= R202), G218 (= G203), S242 (= S228), R243 (= R229), A244 (= A230), Q256 (= Q242), V257 (= V243), G258 (= G244), T260 (= T246), G273 (= G259), I274 (= I260), S275 (= S261), A277 (= A263), Q279 (= Q265), H280 (= H266), N294 (= N280), K295 (= K281), D312 (= D298), V313 (≠ L299)
Sites not aligning to the query:
4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
34% identity, 83% coverage: 53:310/311 of query aligns to 66:332/338 of 4kpuA
- binding flavin-adenine dinucleotide: L125 (≠ Q110), R144 (= R125), I155 (= I135), G224 (= G201), R225 (= R202), G226 (= G203), S250 (= S228), R251 (= R229), A252 (= A230), Q264 (= Q242), V265 (= V243), G266 (= G244), Q267 (= Q245), S268 (≠ T246), G281 (= G259), I282 (= I260), S283 (= S261), S285 (≠ A263), Q287 (= Q265), H288 (= H266), N302 (= N280), K303 (= K281), D320 (= D298), A321 (≠ L299)
7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 98% coverage: 5:310/311 of query aligns to 15:331/337 of 7qh2A
- binding flavin-adenine dinucleotide: L125 (≠ Q110), T126 (≠ L111), R144 (= R125), I155 (= I135), R224 (= R202), G225 (= G203), T249 (≠ S228), R250 (= R229), Q263 (= Q242), I264 (≠ V243), G265 (= G244), L266 (≠ Q245), S267 (≠ T246), G280 (= G259), I281 (= I260), S282 (= S261), Q286 (= Q265), N301 (= N280), S302 (≠ K281), D303 (= D282), D319 (= D298), L320 (= L299)
7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
62% identity, 32% coverage: 212:310/311 of query aligns to 229:327/336 of 7koeB
- binding flavin-adenine dinucleotide: S245 (= S228), R246 (= R229), A247 (= A230), Q259 (= Q242), V260 (= V243), G261 (= G244), Q262 (= Q245), T263 (= T246), G276 (= G259), S278 (= S261), Q282 (= Q265), H283 (= H266), N297 (= N280), I298 (≠ K281), L316 (= L299)
Sites not aligning to the query:
P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
31% identity, 99% coverage: 4:310/311 of query aligns to 5:319/321 of P53571
- R211 (= R202) binding FAD
- SR 236:237 (= SR 228:229) binding FAD
- QVGQS 250:254 (≠ QVGQT 242:246) binding FAD
- 268:275 (vs. 259:266, 88% identical) binding FAD
- N289 (= N280) binding FAD
- DI 307:308 (≠ DL 298:299) binding FAD
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
30% identity, 99% coverage: 4:310/311 of query aligns to 4:318/319 of 3clrD
- binding flavin-adenine dinucleotide: G209 (= G201), R210 (= R202), G211 (= G203), S235 (= S228), A236 (≠ R229), P237 (≠ A230), Q249 (= Q242), V250 (= V243), G251 (= G244), Q252 (= Q245), S253 (≠ T246), G267 (= G259), I268 (= I260), S269 (= S261), S271 (≠ A263), Q273 (= Q265), H274 (= H266), N288 (= N280), T289 (≠ K281), D306 (= D298), I307 (≠ L299)
Query Sequence
>RR42_RS04575 FitnessBrowser__Cup4G11:RR42_RS04575
MTALVIAEHDNQSIKAATLNTVTAAAQCGGDVHVLVAGANAAAAAAAAAKIAGVSKVLLA
DAPQFADGLAENVAEQVLAIASDYSHILAPASAYGKNILPRVAAKLDVAQLSDITKVDSP
DTFERPIYAGNAISIVQSSDKVKVITVRGTGFDAAAADGGSAAVENLPAVADAGISQFVS
REVTKSDRPELTAAKIIVSGGRGVGSGENYTKVLTPLADKLNAAMGASRAAVDAGFVPND
YQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDAEAPIFSVADYGLVGDLN
TVVPELVAALG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory