SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS05080 FitnessBrowser__Cup4G11:RR42_RS05080 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 16 hits to proteins with known functional sites (download)

Q83V26 3-hydroxyanthranilate 3,4-dioxygenase; 3-hydroxyanthranilate oxygenase; 3-HAO; 3-hydroxyanthranilic acid dioxygenase; HAD; EC 1.13.11.6 from Pseudomonas fluorescens (see paper)
76% identity, 96% coverage: 1:179/186 of query aligns to 2:180/185 of Q83V26

query
sites
Q83V26

M

M

F

F

I

T

Y

F

G

G

M

K

P

P

L

L

N

N

F

F

P

Q

R

R

W

W

L

L

D

D

E

D

H

H

A

S

H

D

L

L

K

R

P

P

V

V

G

G

N

N

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

G

G

P

P

N

N

Q

F

R

R

T

T

D

D

F

F

H
|
H

D

D

P

P

L

M

E

E

F

F

Y

Y

Q

Q

F

F

K

K

G

G

N

N

A

A

W

Y

L

L

D

N

I

I

M

M

D

D

R

R

G

G

K

Q

R

M

E

D

R

R

V

V

D

E

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

L

R

R

H
|
H

S

S

P

P

Q

Q

R

R

P

P

E

E

A

A

D

G

S

S

R

R

C

C

L

L

V

V

I

I

E

E

R

R

Q

Q

R

R

P

P

E

K

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C

C

P

L

A

S

C

C

S

N

A

G

L

L

V

V

Y

Y

R

R

V

V

E

D

V

V

Q

Q

L

L

K

N

S

S

I

I

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

D

G

I

F

F

Y

Y

D

G

N

N

A

V

A

G

L

L

R

R

R

K

C

C

G

P

Q

Q

C

C

G

G

T

Q

V

V

H

H

P

P

G

G

R

K

A

A

S

A

A

I

P

E

A

A

P

V

A

A

H52 (= H51) mutation to A: Loss of activity.
H96 (= H95) mutation to A: Loss of activity.

6vibA Observing a ring-cleaving dioxygenase in action through a crystalline lens - enol tautomers of acms bidentately bound structure (see paper)
76% identity, 94% coverage: 1:174/186 of query aligns to 2:175/175 of 6vibA

query
sites
6vibA

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V

V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

V
|
V

G

G

P

P

N

N

Q

H

R

R

T

T

D

D

F

Y

H
|
H

D

D

P

P

L

L

E

E

E
|
E

F

F

F
|
F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H
|
H

S

S

P
|
P

Q

Q

R
|
R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V
|
V

I

I

E
|
E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C
|
C

P

D

A

A

C
|
C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I
|
I

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C
|
C

G

P

Q

H

C
|
C

G

G

T

Q

V

V

H

H

P

P

G

G

R

R

A

A

S

A

binding (2Z,3Z)-2-[(2Z)-3-hydroxyprop-2-en-1-ylidene]-3-iminobutanedioic acid: V42 (= V41), H52 (= H51), E58 (= E57), F60 (= F59), H96 (= H95), P98 (= P97), R100 (= R99), V109 (= V108), E111 (= E110), I143 (= I142)
binding fe (ii) ion: H52 (= H51), E58 (= E57), H96 (= H95), C126 (= C125), C129 (= C128), C163 (= C162), C166 (= C165)

6viaA Observing a ring-cleaving dioxygenase in action through a crystalline lens - a seven-membered lactone bound structure (see paper)
76% identity, 94% coverage: 1:174/186 of query aligns to 3:176/176 of 6viaA

query
sites
6viaA

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V

V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

V
|
V

G

G

P

P

N

N

Q

H

R

R

T

T

D

D

F

Y

H
|
H

D

D

P

P

L

L

E

E

E
|
E

F

F

F
|
F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H
|
H

S

S

P
|
P

Q

Q

R
|
R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V

V

I

I

E
|
E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C
|
C

P

D

A

A

C
|
C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I

I

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C
|
C

G

P

Q

H

C
|
C

G

G

T

Q

V

V

H

H

P

P

G

G

R

R

A

A

S

A

binding (2R,3E)-2-hydroxy-3-imino-2,3-dihydrooxepine-4-carboxylic acid: V43 (= V41), H53 (= H51), E59 (= E57), F61 (= F59), P99 (= P97), R101 (= R99), E112 (= E110)
binding fe (iii) ion: H53 (= H51), E59 (= E57), H97 (= H95)
binding fe (ii) ion: C127 (= C125), C130 (= C128), C164 (= C162), C167 (= C165)

6vi8A Observing a ring-cleaving dioxygenase in action through a crystalline lens - a superoxo bound structure (see paper)
76% identity, 94% coverage: 1:174/186 of query aligns to 3:176/176 of 6vi8A

query
sites
6vi8A

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V

V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

G

G

P

P

N

N

Q

H

R

R

T

T

D

D

F

Y

H
|
H

D

D

P

P

L

L

E

E

E
|
E

F

F

F
|
F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H
|
H

S

S

P
|
P

Q

Q

R
|
R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V

V

I

I

E
|
E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C
|
C

P

D

A

A

C
|
C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I

I

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C
|
C

G

P

Q

H

C
|
C

G

G

T

Q

V

V

H

H

P

P

G

G

R

R

A

A

S

A

binding 3-hydroxyanthranilic acid: H53 (= H51), E59 (= E57), F61 (= F59), P99 (= P97), R101 (= R99), E112 (= E110)
binding fe (iii) ion: H53 (= H51), E59 (= E57), H97 (= H95)
binding fe (ii) ion: C127 (= C125), C130 (= C128), C164 (= C162), C167 (= C165)
binding oxygen atom: H53 (= H51), H97 (= H95)

4i3pA 1.96 angstrom x-ray crystal structure of 3-hydroxyanthranilate-3,4- dioxygenase bound with 3-aminosalicylic acid from cupriavidus metallidurans
76% identity, 94% coverage: 1:174/186 of query aligns to 1:174/174 of 4i3pA

query
sites
4i3pA

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V

V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

G

G

P

P

N

N

Q

H

R
|
R

T

T

D

D

F

Y

H
|
H

D

D

P

P

L

L

E

E

E
|
E

F

F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H
|
H

S

S

P
|
P

Q

Q

R
|
R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V
|
V

I

I

E
|
E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C
|
C

P

D

A

A

C
|
C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I

I

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C
|
C

G

P

Q

H

C
|
C

G

G

T

Q

V

V

H

H

P

P

G

G

R

R

A

A

S

A

binding 3-amino-2-hydroxybenzoic acid: R47 (= R47), E57 (= E57), P97 (= P97), R99 (= R99), V108 (= V108), E110 (= E110)
binding fe (ii) ion: H51 (= H51), E57 (= E57), H95 (= H95), C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)

4hvoA 1.75 angstrom x-ray crystal structure of cufe reconstituted 3- hydroxyanthranilate-3,4-dioxygenase from cupriavidus metallidurans (see paper)
76% identity, 94% coverage: 1:174/186 of query aligns to 1:174/174 of 4hvoA

query
sites
4hvoA

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V

V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

G

G

P

P

N

N

Q

H

R

R

T

T

D

D

F

Y

H
|
H

D

D

P

P

L

L

E

E

E
|
E

F

F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H
|
H

S

S

P

P

Q

Q

R

R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V

V

I

I

E

E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C
|
C

P

D

A

A

C
|
C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I

I

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C
|
C

G

P

Q

H

C
|
C

G

G

T

Q

V

V

H

H

P

P

G

G

R

R

A

A

S

A

binding copper (ii) ion: H51 (= H51), E57 (= E57), H95 (= H95)
binding fe (ii) ion: C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)

4hsjA 1.88 angstrom x-ray crystal structure of piconlinic-bound 3- hydroxyanthranilate-3,4-dioxygenase (see paper)
76% identity, 94% coverage: 1:174/186 of query aligns to 1:174/174 of 4hsjA

query
sites
4hsjA

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V

V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

G

G

P

P

N

N

Q

H

R

R

T

T

D

D

F

Y

H
|
H

D

D

P

P

L

L

E

E

E
|
E

F

F

F
|
F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H
|
H

S

S

P
|
P

Q

Q

R

R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V

V

I

I

E
|
E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C
|
C

P

D

A

A

C
|
C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I

I

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C
|
C

G

P

Q

H

C
|
C

G

G

T

Q

V

V

H

H

P

P

G

G

R

R

A

A

S

A

binding pyridine-2-carboxylic acid: H51 (= H51), E57 (= E57), F59 (= F59), H95 (= H95), P97 (= P97), E110 (= E110)
binding fe (ii) ion: H51 (= H51), E57 (= E57), H95 (= H95), C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)

1yfxA Crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase from ralstonia metallidurans complexed with 4-chloro-3-hydroxyanthranilic acid and no (see paper)
76% identity, 94% coverage: 1:174/186 of query aligns to 1:174/174 of 1yfxA

query
sites
1yfxA

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V
|
V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

G

G

P

P

N

N

Q

H

R
|
R

T

T

D

D

F

Y

H
|
H

D

D

P

P

L

L

E

E

E
|
E

F

F

F
|
F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H
|
H

S

S

P
|
P

Q

Q

R
|
R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V

V

I

I

E
|
E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C
|
C

P

D

A

A

C
|
C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I
|
I

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C
|
C

G

P

Q

H

C
|
C

G

G

T

Q

V

V

H

H

P

P

G

G

R

R

A

A

S

A

binding 4-chloro-3-hydroxyanthranilic acid: V25 (= V25), R47 (= R47), H51 (= H51), E57 (= E57), F59 (= F59), P97 (= P97), R99 (= R99), E110 (= E110), I142 (= I142)
binding fe (iii) ion: H51 (= H51), E57 (= E57), H95 (= H95), C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)
binding nitric oxide: R47 (= R47), H51 (= H51), H95 (= H95), P97 (= P97)

1yfwA Crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase from ralstonia metallidurans complexed with 4-chloro-3-hydroxyanthranilic acid and o2 (see paper)
76% identity, 94% coverage: 1:174/186 of query aligns to 1:174/174 of 1yfwA

query
sites
1yfwA

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V
|
V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

G

G

P

P

N

N

Q

H

R
|
R

T

T

D

D

F

Y

H
|
H

D

D

P

P

L

L

E

E

E
|
E

F

F

F
|
F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H
|
H

S

S

P
|
P

Q

Q

R
|
R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V

V

I

I

E
|
E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C
|
C

P

D

A

A

C
|
C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I
|
I

V

V

T

T

D

D

L
|
L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C
|
C

G

P

Q

H

C
|
C

G

G

T

Q

V

V

H

H

P

P

G

G

R

R

A

A

S

A

binding 4-chloro-3-hydroxyanthranilic acid: V25 (= V25), H51 (= H51), E57 (= E57), F59 (= F59), P97 (= P97), R99 (= R99), E110 (= E110), I142 (= I142), L146 (= L146)
binding fe (iii) ion: H51 (= H51), E57 (= E57), H95 (= H95), C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)
binding oxygen molecule: R47 (= R47), H51 (= H51), H95 (= H95), P97 (= P97)

Q1LCS4 3-hydroxyanthranilate 3,4-dioxygenase; 3-hydroxyanthranilate oxygenase; 3-HAO; 3-hydroxyanthranilic acid dioxygenase; HAD; EC 1.13.11.6 from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) (Ralstonia metallidurans) (see paper)
76% identity, 94% coverage: 1:174/186 of query aligns to 1:174/174 of Q1LCS4

query
sites
Q1LCS4

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V

V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

G

G

P

P

N

N

Q

H

R
|
R

T

T

D

D

F

Y

H

H

D

D

P

P

L

L

E

E

F

F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H

H

S

S

P

P

Q

Q

R
|
R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V

V

I

I

E
|
E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C

C

P

D

A

A

C

C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I

I

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C

C

G

P

Q

H

C

C

G

G

T

Q

V

V

H

H

P

P

G

G

R

R

A

A

S

A

R47 (= R47) mutation to A: Increases KM for 3-hydroxyanthranilate 7-fold. Decreases activity 1000-fold.
R99 (= R99) mutation to A: Increases KM for 3-hydroxyanthranilate 40-fold. Decreases activity 5000-fold.
E110 (= E110) mutation to A: Decreases KM for 3-hydroxyanthranilate 2-fold. Decreases activity 2000-fold.

4wzcA Understanding extradiol dioxygenase mechanism in NAD+ biosynthesis by viewing catalytic intermediates - 2,3-cis-4,5-trans acms bound to i142a mutant hao
76% identity, 94% coverage: 1:174/186 of query aligns to 1:174/174 of 4wzcA

query
sites
4wzcA

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V

V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

G

G

P

P

N

N

Q

H

R
|
R

T

T

D

D

F

Y

H
|
H

D

D

P

P

L

L

E

E

E
|
E

F

F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H
|
H

S

S

P
|
P

Q

Q

R
|
R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V

V

I

I

E
|
E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C
|
C

P

D

A

A

C
|
C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I

A

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C
|
C

G

P

Q

H

C
|
C

G

G

T

Q

V

V

H

H

P

P

G

G

R

R

A

A

S

A

binding (2E)-2-amino-3-[(1E)-3-oxoprop-1-en-1-yl]but-2-enedioic acid: R47 (= R47), H51 (= H51), E57 (= E57), H95 (= H95), P97 (= P97), R99 (= R99), E110 (= E110)
binding fe (ii) ion: H51 (= H51), E57 (= E57), H95 (= H95), C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)

6vi9A Observing a ring-cleaving dioxygenase in action through a crystalline lens - an alkylperoxo bound structure (see paper)
77% identity, 92% coverage: 1:171/186 of query aligns to 1:171/171 of 6vi9A

query
sites
6vi9A

M

M

F

L

I

T

Y

Y

G

G

M

A

P

P

L

F

N

N

F

F

P

P

R

R

W

W

L

I

D

D

E

E

H

H

A

A

H

H

L

L

K

K

P

P

V

V

G

G

N

N

Q

R

Q

Q

V

V

W

W

Q

Q

D

D

A

S

D

D

T

F

I

I

V

V

T

T

V

V

G

G

P

P

N

N

Q

H

R
|
R

T

T

D

D

F

Y

H
|
H

D

D

P

P

L

L

E

E

E
|
E

F

F

F
|
F

Y

Y

Q

Q

F

L

K

R

G

G

N

N

A

A

W

Y

L

L

D

N

I

L

M

W

D

V

R

D

G

G

K

R

R

R

E

E

R

R

V

A

D

D

L

L

K

K

E

E

G

G

D

D

I

I

F

F

L

L

P

P

A

P

H

H

V

V

R

R

H
|
H

S

S

P
|
P

Q

Q

R
|
R

P

P

E

E

A

A

D

G

S

S

R

A

C

C

L

L

V

V

I

I

E
|
E

R

R

Q

Q

R

R

P

P

E

A

G

G

V

M

V

L

D

D

G

G

F

F

E

E

W

W

Y

Y

C
|
C

P

D

A

A

C
|
C

S

G

A

H

L

L

V

V

Y

H

R

R

V

V

E

E

V

V

Q

Q

L

L

K

K

S

S

I

I

V

V

T

T

D

D

L

L

P

P

L

L

F

F

A

E

G

S

F

F

Y

Y

D

A

N

S

A

E

A

D

L

K

R

R

C
|
C

G

P

Q

H

C
|
C

G

G

T

Q

V

V

H

H

P

P

G

G

binding fe (ii) ion: C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)
binding (5R,6Z)-5-(hydroperoxy-kappaO)-5-(hydroxy-kappaO)-6-iminocyclohexa-1,3-diene-1-carboxylato(2-)iron: R47 (= R47), H51 (= H51), E57 (= E57), F59 (= F59), H95 (= H95), P97 (= P97), R99 (= R99), E110 (= E110)

3fe5A Crystal structure of 3-hydroxyanthranilate 3,4-dioxygenase from bovine kidney (see paper)
35% identity, 95% coverage: 7:182/186 of query aligns to 3:172/283 of 3fe5A

query
sites
3fe5A

P

P

L

V

N

R

F

V

P

K

R

A

W

W

L

V

D

E

E

E

H

N

A

R

H

G

L

S

L

F

K

L

P

P

V

V

G

C

N

N

Q

K

Q

L

V

L

W

H

Q

Q

D

K

A

Q

D

L

T

K

I

I

V

M

T

-

V

F

V

V

G

G

P

P

N

N

Q

T

R

R

T

K

D

D

F

Y

H
|
H

D

I

D

E

P

E

L

G

E

E

E
|
E

F

V

F

F

Y

Y

Q

Q

F

L

K

E

G

G

N

D

A

M

W

L

L

L

D

R

I

V

M

L

D

E

R

R

G

G

K

K

R

H

E

R

R

D

V

V

D

V

L

I

K

R

E

Q

G

G

D

E

I

I

F

F

L

L

P

P

A

A

H

G

V

V

R

P

H
|
H

S

S

P

P

Q

Q

R

R

P

F

E

-

A

A

D

N

S

T

R

V

C

G

L

L

V

V

I

I

E

E

R

R

Q

R

R

R

P

L

E

K

G

T

V

E

V

L

D

D

G

G

F

L

E

R

W

Y

Y

Y

C

V

P

G

A

D

C

T

S

T

A

D

L

V

V

L

Y

F

R

E

V

K

E

W

V

F

Q

Y

L

C

K

E

S

D

I

L

V

G

T

T

D

Q

L

L

P

A

P

P

L

I

F

I

A

Q

G

E

F

F

Y

F

D

S

N

S

A

E

A

Q

L

Y

R

R

R

-

C

-

G

-

Q

-

C

T

G

G

T

K

V

P

H

N

P

P

G

D

R

Q

A

L

S

L

A

K

P

E

A

P

P

P

A

F

P

P

A

L

S

S

binding fe (iii) ion: H46 (= H51), E52 (= E57), H90 (= H95)

5tkqA Crystal structure of human 3hao with zinc bound in the active site (see paper)
36% identity, 82% coverage: 8:160/186 of query aligns to 3:153/283 of 5tkqA

query
sites
5tkqA

L

L

N

G

F

V

P

R

R

A

W

W

L

V

D

K

E

E

H

N

A

R

H

G

L

S

L

F

K

Q

P

P

V

V

G

C

N

N

Q

K

Q

L

V

M

W

H

Q

Q

D

E

A

-

D

Q

T

L

I

K

V

V

T

M

V

F

V

I

G

G

P

P

N

N

Q

T

R

R

T

K

D

D

F

Y

H
|
H

D

I

D

E

P

E

L

G

E

E

E
|
E

F

V

F

F

Y

Y

Q

Q

F

L

K

E

G

G

N

D

A

M

W

V

L

L

D

R

I

V

M

L

D

E

R

Q

G

G

K

K

R

H

E

R

R

D

V

V

D

V

L

I

K

R

E

Q

G

G

D

E

I

I

F

F

L

L

P

P

A

A

H

R

V

V

R

P

H
|
H

S

S

P

P

Q

Q

R

R

P

-

E

F

A

A

D

N

S

T

R

V

C

G

L

L

V

V

I

V

E

E

R

R

Q

R

R

R

P

L

E

E

G

T

V

E

V

L

D

D

G

G

F

L

E

R

W

Y

Y

Y

C

V

P

G

A

D

C

T

S

M

A

D

L

V

V

L

Y

F

R

E

V

K

E

W

V

F

Q

Y

L

C

K

K

S

D

I

L

V

G

T

T

D

Q

L

L

P

A

P

P

L

I

F

I

A

Q

G

E

F

F

Y

F

D

S

N

S

A

E

A

Q

L

Y

R

R

binding zinc ion: H45 (= H51), E51 (= E57), H89 (= H95)

5tk5A Crystal structure of human 3hao with iron bound in the active site (see paper)
36% identity, 82% coverage: 8:160/186 of query aligns to 3:153/283 of 5tk5A

query
sites
5tk5A

L

L

N

G

F

V

P

R

R

A

W

W

L

V

D

K

E

E

H

N

A

R

H

G

L

S

L

F

K

Q

P

P

V

V

G

C

N

N

Q

K

Q

L

V

M

W

H

Q

Q

D

E

A

-

D

Q

T

L

I

K

V

V

T

M

V

F

V

I

G

G

P

P

N

N

Q

T

R

R

T

K

D

D

F

Y

H
|
H

D

I

D

E

P

E

L

G

E

E

E
|
E

F

V

F

F

Y

Y

Q

Q

F

L

K

E

G

G

N

D

A

M

W

V

L

L

D

R

I

V

M

L

D

E

R

Q

G

G

K

K

R

H

E

R

R

D

V

V

D

V

L

I

K

R

E

Q

G

G

D

E

I

I

F

F

L

L

P

P

A

A

H

R

V

V

R

P

H
|
H

S

S

P

P

Q

Q

R

R

P

-

E

F

A

A

D

N

S

T

R

V

C

G

L

L

V

V

I

V

E

E

R

R

Q

R

R

R

P

L

E

E

G

T

V

E

V

L

D

D

G

G

F

L

E

R

W

Y

Y

Y

C

V

P

G

A

D

C

T

S

M

A

D

L

V

V

L

Y

F

R

E

V

K

E

W

V

F

Q

Y

L

C

K

K

S

D

I

L

V

G

T

T

D

Q

L

L

P

A

P

P

L

I

F

I

A

Q

G

E

F

F

Y

F

D

S

N

S

A

E

A

Q

L

Y

R

R

binding fe (iii) ion: H45 (= H51), E51 (= E57), H89 (= H95)

P46952 3-hydroxyanthranilate 3,4-dioxygenase; 3-hydroxyanthranilate oxygenase; 3-HAO; h3HAO; 3-hydroxyanthranilic acid dioxygenase; HAD; EC 1.13.11.6 from Homo sapiens (Human) (see 3 papers)
36% identity, 82% coverage: 8:160/186 of query aligns to 5:155/286 of P46952

query
sites
P46952

L

L

N

G

F

V

P

R

R

A

W

W

L

V

D

K

E

E

H

N

A

R

H

G

L

S

L

F

K

Q

P

P

V

V

G

C

N

N

Q

K

Q

L

V

M

W

H

Q

Q

D

E

A

-

D

Q

T

L

I

K

V

V

T

M

V

F

V
x
I

G

G

P

P

N

N

Q

T

R

R

T

K

D

D

F

Y

H
|
H

D

I

D

E

P

E

L

G

E

E

E
|
E

F

V

F

F

Y

Y

Q

Q

F

L

K

E

G

G

N

D

A

M

W

V

L

L

D

R

I

V

M

L

D

E

R

Q

G

G

K

K

R

H

E

R

R

D

V

V

D

V

L

I

K

R

E

Q

G

G

D

E

I

I

F

F

L

L

P

P

A

A

H

R

V

V

R

P

H
|
H

S

S

P

P

Q

Q

R

R

P

F

E

-

A

A

D

N

S

T

R

V

C

G

L

L

V

V

I

V

E

E

R

R

Q

R

R

R

P

L

E

E

G

T

V

E

V

L

D

D

G

G

F

L

E

R

W

Y

Y

Y

C

V

P

G

A

D

C

T

S

M

A

D

L

V

V

L

Y

F

R

E

V

K

E

W

V

F

Q

Y

L

C

K

K

S

D

I

L

V

G

T

T

D

Q

L

L

P

A

P

P

L

I

F

I

A

Q

G

E

F

F

Y

F

D

S

N

S

A

E

A

Q

L

Y

R

R

I37 (≠ V41) to V: in dbSNP:rs3816183
H47 (= H51) binding
E53 (= E57) binding
H91 (= H95) binding

Sites not aligning to the query:

162:286 natural variant: Missing (in VCRL1; strongly reduced 3-hydroxyanthranilate 3,4-dioxygenase activity in vitro)
186:286 natural variant: Missing (in VCRL1; strongly reduced 3-hydroxyanthranilate 3,4-dioxygenase activity in vitro)

Query Sequence

>RR42_RS05080 FitnessBrowser__Cup4G11:RR42_RS05080
MFIYGMPLNFPRWLDEHAHLLKPPVGNQQVWQDADTIVTVVGGPNQRTDFHDDPLEEFFY
QFKGNAWLDIMDRGKRERVDLKEGDIFLLPAHVRHSPQRPEADSRCLVIERQRPEGVVDG
FEWYCPACSALVYRVEVQLKSIVTDLPPLFAGFYDNAALRRCGQCGTVHPGRASAPAPAP
ASKDPE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory