SitesBLAST
Comparing RR42_RS05755 FitnessBrowser__Cup4G11:RR42_RS05755 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
33% identity, 94% coverage: 16:259/259 of query aligns to 17:257/259 of 5zaiC
- active site: A65 (≠ G64), F70 (≠ L69), S82 (= S83), R86 (≠ N88), G110 (≠ A112), E113 (≠ S115), P132 (≠ A134), E133 (≠ Y135), I138 (≠ L140), P140 (= P142), G141 (≠ D143), A226 (= A228), F236 (≠ L238)
- binding coenzyme a: K24 (≠ A23), L25 (≠ R24), A63 (= A62), G64 (= G63), A65 (≠ G64), D66 (≠ N65), I67 (≠ L66), P132 (≠ A134), R166 (≠ P168), F248 (= F250), K251 (= K253)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
38% identity, 98% coverage: 5:259/259 of query aligns to 5:255/257 of 6slbAAA
- active site: Q64 (≠ G64), F69 (≠ L69), L80 (≠ I84), N84 (= N88), A108 (= A112), S111 (= S115), A130 (= A134), F131 (≠ Y135), L136 (= L140), P138 (= P142), D139 (= D143), A224 (= A228), G234 (≠ L238)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ G58), A62 (= A62), Q64 (≠ G64), D65 (≠ N65), L66 (= L66), Y76 (≠ R77), A108 (= A112), F131 (≠ Y135), D139 (= D143)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
35% identity, 98% coverage: 5:258/259 of query aligns to 5:249/250 of 3q0gD
- active site: A64 (≠ G64), M69 (≠ L69), T75 (≠ S83), F79 (≠ L87), G103 (≠ A112), E106 (≠ S115), P125 (≠ A134), E126 (≠ Y135), V131 (≠ L140), P133 (= P142), G134 (≠ D143), L219 (≠ A228), F229 (≠ L238)
- binding Butyryl Coenzyme A: F225 (= F234), F241 (= F250)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
35% identity, 98% coverage: 5:258/259 of query aligns to 6:254/255 of 3q0jC
- active site: A65 (≠ G64), M70 (≠ L69), T80 (≠ S83), F84 (≠ L87), G108 (≠ A112), E111 (≠ S115), P130 (≠ A134), E131 (≠ Y135), V136 (≠ L140), P138 (= P142), G139 (≠ D143), L224 (≠ A228), F234 (≠ L238)
- binding acetoacetyl-coenzyme a: Q23 (≠ E22), A24 (= A23), L25 (≠ R24), A27 (= A26), A63 (= A62), G64 (= G63), A65 (≠ G64), D66 (≠ N65), I67 (≠ L66), K68 (≠ N67), M70 (≠ L69), F84 (≠ L87), G107 (= G111), G108 (≠ A112), E111 (≠ S115), P130 (≠ A134), E131 (≠ Y135), P138 (= P142), G139 (≠ D143), M140 (≠ G144)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
35% identity, 98% coverage: 5:258/259 of query aligns to 6:254/255 of 3q0gC
- active site: A65 (≠ G64), M70 (≠ L69), T80 (≠ S83), F84 (≠ L87), G108 (≠ A112), E111 (≠ S115), P130 (≠ A134), E131 (≠ Y135), V136 (≠ L140), P138 (= P142), G139 (≠ D143), L224 (≠ A228), F234 (≠ L238)
- binding coenzyme a: L25 (≠ R24), A63 (= A62), I67 (≠ L66), K68 (≠ N67), Y104 (≠ P108), P130 (≠ A134), E131 (≠ Y135), L134 (≠ V138)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
35% identity, 98% coverage: 5:258/259 of query aligns to 5:253/256 of 3h81A
- active site: A64 (≠ G64), M69 (≠ L69), T79 (≠ S83), F83 (≠ L87), G107 (≠ A112), E110 (≠ S115), P129 (≠ A134), E130 (≠ Y135), V135 (≠ L140), P137 (= P142), G138 (≠ D143), L223 (≠ A228), F233 (≠ L238)
- binding calcium ion: F233 (≠ L238), Q238 (≠ G243)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
33% identity, 98% coverage: 6:259/259 of query aligns to 11:264/266 of O53561
- K135 (= K130) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 130:137, 25% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ N137) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
36% identity, 98% coverage: 5:259/259 of query aligns to 2:243/245 of 6slaAAA
- active site: Q61 (≠ G64), L68 (≠ I84), N72 (= N88), A96 (= A112), S99 (= S115), A118 (= A134), F119 (≠ Y135), L124 (= L140), P126 (= P142), N127 (≠ D143), A212 (= A228), G222 (≠ L238)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ R24), A59 (= A62), Q61 (≠ G64), D62 (≠ N65), L63 (= L66), L68 (≠ I84), Y71 (≠ L87), A94 (= A110), G95 (= G111), A96 (= A112), F119 (≠ Y135), I122 (≠ V138), L124 (= L140), N127 (≠ D143), F234 (= F250), K237 (= K253)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
31% identity, 96% coverage: 11:259/259 of query aligns to 15:258/260 of 2hw5C
- active site: A68 (≠ G64), M73 (≠ L69), S83 (= S83), L87 (= L87), G111 (≠ A112), E114 (≠ S115), P133 (≠ A134), E134 (≠ Y135), T139 (≠ L140), P141 (= P142), G142 (≠ D143), K227 (≠ A228), F237 (≠ L238)
- binding crotonyl coenzyme a: K26 (≠ E22), A27 (= A23), L28 (≠ R24), A30 (= A26), K62 (≠ G58), I70 (≠ L66), F109 (≠ A110)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
32% identity, 95% coverage: 15:259/259 of query aligns to 17:256/258 of 1ey3A
- active site: A66 (≠ G64), M71 (≠ L69), S81 (= S83), L85 (= L87), G109 (≠ A112), E112 (≠ S115), P131 (≠ A134), E132 (≠ Y135), T137 (≠ L140), P139 (= P142), G140 (≠ D143), K225 (≠ A228), F235 (≠ L238)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E22), L26 (≠ R24), A28 (= A26), A64 (= A62), G65 (= G63), A66 (≠ G64), D67 (≠ N65), I68 (≠ L66), L85 (= L87), W88 (= W90), G109 (≠ A112), P131 (≠ A134), L135 (≠ V138), G140 (≠ D143)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
32% identity, 95% coverage: 15:259/259 of query aligns to 19:258/260 of 1dubA
- active site: A68 (≠ G64), M73 (≠ L69), S83 (= S83), L87 (= L87), G111 (≠ A112), E114 (≠ S115), P133 (≠ A134), E134 (≠ Y135), T139 (≠ L140), P141 (= P142), G142 (≠ D143), K227 (≠ A228), F237 (≠ L238)
- binding acetoacetyl-coenzyme a: K26 (≠ E22), A27 (= A23), L28 (≠ R24), A30 (= A26), A66 (= A62), A68 (≠ G64), D69 (≠ N65), I70 (≠ L66), Y107 (≠ P108), G110 (= G111), G111 (≠ A112), E114 (≠ S115), P133 (≠ A134), E134 (≠ Y135), L137 (≠ V138), G142 (≠ D143), F233 (= F234), F249 (= F250)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
32% identity, 95% coverage: 15:259/259 of query aligns to 49:288/290 of P14604
- E144 (≠ S115) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ Y135) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
31% identity, 95% coverage: 15:259/259 of query aligns to 19:256/258 of 1mj3A
- active site: A68 (≠ G64), M73 (≠ L69), S83 (≠ E85), L85 (= L87), G109 (≠ A112), E112 (≠ S115), P131 (≠ A134), E132 (≠ Y135), T137 (≠ L140), P139 (= P142), G140 (≠ D143), K225 (≠ A228), F235 (≠ L238)
- binding hexanoyl-coenzyme a: K26 (≠ E22), A27 (= A23), L28 (≠ R24), A30 (= A26), A66 (= A62), G67 (= G63), A68 (≠ G64), D69 (≠ N65), I70 (≠ L66), G109 (≠ A112), P131 (≠ A134), E132 (≠ Y135), L135 (≠ V138), G140 (≠ D143)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
31% identity, 95% coverage: 15:259/259 of query aligns to 18:252/254 of 2dubA
- active site: A67 (≠ G64), M72 (≠ L69), S82 (≠ N88), G105 (≠ A112), E108 (≠ S115), P127 (≠ A134), E128 (≠ Y135), T133 (≠ L140), P135 (= P142), G136 (≠ D143), K221 (≠ A228), F231 (≠ L238)
- binding octanoyl-coenzyme a: K25 (≠ E22), A26 (= A23), L27 (≠ R24), A29 (= A26), A65 (= A62), A67 (≠ G64), D68 (≠ N65), I69 (≠ L66), K70 (≠ N67), G105 (≠ A112), E108 (≠ S115), P127 (≠ A134), E128 (≠ Y135), G136 (≠ D143), A137 (≠ G144)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
36% identity, 95% coverage: 15:259/259 of query aligns to 17:259/261 of 5jbxB
- active site: A67 (≠ G64), R72 (= R77), L84 (= L87), R88 (≠ I91), G112 (≠ A112), E115 (≠ S115), T134 (≠ A134), E135 (≠ Y135), I140 (≠ L140), P142 (= P142), G143 (≠ D143), A228 (= A228), L238 (= L238)
- binding coenzyme a: S24 (≠ E22), R25 (≠ A23), R26 (= R24), A28 (= A26), A65 (= A62), D68 (≠ N65), L69 (= L66), K70 (= K75), L110 (≠ A110), G111 (= G111), T134 (≠ A134), E135 (≠ Y135), L138 (≠ V138), R168 (≠ P168)
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
33% identity, 95% coverage: 14:259/259 of query aligns to 22:266/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
7borA Structure of pseudomonas aeruginosa coa-bound odaa (see paper)
32% identity, 95% coverage: 8:253/259 of query aligns to 7:247/247 of 7borA
- active site: N63 (= N67), F68 (≠ N72), D77 (≠ A81), G81 (≠ N88), I105 (≠ A112), T108 (≠ S115), F128 (≠ Y135), L133 (= L140), P135 (= P142), E136 (≠ D143), A222 (= A228), L232 (= L238)
- binding coenzyme a: D21 (≠ E22), K22 (≠ A23), A25 (= A26), S61 (≠ A62), I65 (≠ L69), V103 (≠ A110), F128 (≠ Y135), L131 (≠ V138), F244 (= F250), R247 (≠ K253)
4elxA Structure of apo e.Coli. 1,4-dihydroxy-2- naphthoyl coa synthases with cl (see paper)
31% identity, 96% coverage: 10:258/259 of query aligns to 28:261/268 of 4elxA
- active site: G83 (= G64), H88 (≠ S83), L92 (= L87), G116 (≠ A112), V119 (≠ S115), G139 (≠ Y135), S144 (≠ L140), D146 (≠ P142), G147 (≠ D143), A233 (≠ L227), Y241 (≠ V235)
- binding chloride ion: G115 (= G111), G139 (≠ Y135), W167 (≠ I163)
3t88A Crystal structure of escherichia coli menb in complex with substrate analogue, osb-ncoa (see paper)
30% identity, 96% coverage: 10:258/259 of query aligns to 27:274/281 of 3t88A
- active site: G82 (= G64), R87 (≠ L70), Y93 (≠ P76), H101 (≠ S83), L105 (= L87), G129 (≠ A112), V132 (≠ S115), G152 (≠ Y135), S157 (≠ L140), D159 (≠ P142), G160 (≠ D143), A246 (≠ L227), Y254 (≠ V235)
- binding o-succinylbenzoyl-N-coenzyme A: Q39 (≠ E22), V40 (≠ A23), R41 (= R24), A43 (= A26), S80 (≠ A62), G81 (= G63), G82 (= G64), D83 (≠ N65), Q84 (≠ N67), K85 (≠ R68), Y93 (≠ P76), V104 (= V86), L105 (= L87), Y125 (≠ P108), G129 (≠ A112), T151 (≠ A134), V155 (= V138), F158 (≠ T141), D159 (≠ P142), T250 (≠ R231), Y254 (≠ V235), F266 (= F250), K269 (= K253)
4i42A E.Coli. 1,4-dihydroxy-2-naphthoyl coenzyme a synthase (ecmenb) in complex with 1-hydroxy-2-naphthoyl-coa (see paper)
30% identity, 96% coverage: 10:258/259 of query aligns to 31:278/285 of 4i42A
- active site: G86 (= G64), R91 (≠ L70), Y97 (≠ P76), H105 (≠ S83), L109 (= L87), G133 (≠ A112), V136 (≠ S115), G156 (≠ Y135), S161 (≠ L140), D163 (≠ P142), G164 (≠ D143), A250 (≠ L227), Y258 (≠ V235)
- binding 1-hydroxy-2-naphthoyl-CoA: V44 (≠ A23), R45 (= R24), S84 (≠ A62), G85 (= G63), G86 (= G64), D87 (≠ N65), Q88 (≠ N67), K89 (≠ R68), Y97 (≠ P76), V108 (= V86), Y129 (≠ P108), G133 (≠ A112), T155 (≠ A134), S161 (≠ L140), T254 (≠ R231), F270 (= F250), K273 (= K253)
Query Sequence
>RR42_RS05755 FitnessBrowser__Cup4G11:RR42_RS05755
MTAQLLSERVDTTLVLTISNPEARNALHPDIYAASQQALELASRDDEIRAVVITGADGVF
CAGGNLNRLLGNRAKPREVQAESIEVLNHWIETLHAFPKPIIAAVEGPAAGAGFSLVLAC
DFVVAASDAKFVMAYVNVGLTPDGGGSYELARALPRQLASELIMEGKPVDAARLAHFGLV
NRVVPPGQALTEALRLGESLATRSPHAMGRIKGLINHAATASLTEHLAQERDNFVAALHH
KDGGEGIGAFLEKRKPNYR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory