SitesBLAST
Comparing RR42_RS05770 FitnessBrowser__Cup4G11:RR42_RS05770 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1u08A Crystal structure and reactivity of ybdl from escherichia coli identify a methionine aminotransferase function. (see paper)
53% identity, 96% coverage: 18:395/395 of query aligns to 4:382/382 of 1u08A
- binding pyridoxal-5'-phosphate: G95 (= G109), A96 (= A110), T97 (= T111), Y121 (= Y135), N166 (= N180), D198 (= D212), V200 (= V214), Y201 (= Y215), S229 (= S243), K232 (= K246), K240 (= K254)
2o0rA The three-dimensional structure of n-succinyldiaminopimelate aminotransferase from mycobacterium tuberculosis (see paper)
41% identity, 95% coverage: 20:395/395 of query aligns to 4:383/385 of 2o0rA
4wljA High resolution crystal structure of human kynurenine aminotransferase-i in complex with aminooxyacetate (see paper)
34% identity, 95% coverage: 19:393/395 of query aligns to 4:407/413 of 4wljA
- active site: F122 (≠ Y135), D204 (= D212), V206 (= V214), K238 (= K246)
- binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: W15 (≠ F30), G96 (= G109), G97 (≠ A110), Y98 (≠ T111), F122 (≠ Y135), N172 (= N180), N176 (= N184), D204 (= D212), V206 (= V214), Y207 (= Y215), S235 (= S243), K238 (= K246), K246 (= K254), R389 (= R375)
1w7nA Crystal structure of human kynurenine aminotransferase i in pmp form (see paper)
34% identity, 95% coverage: 19:393/395 of query aligns to 4:410/415 of 1w7nA
- active site: F122 (≠ Y135), D207 (= D212), V209 (= V214), K241 (= K246)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G96 (= G109), G97 (≠ A110), Y98 (≠ T111), F122 (≠ Y135), N175 (= N180), D207 (= D212), V209 (= V214), Y210 (= Y215), S238 (= S243), K241 (= K246), K249 (= K254)
1w7mA Crystal structure of human kynurenine aminotransferase i in complex with l-phe (see paper)
34% identity, 95% coverage: 19:393/395 of query aligns to 4:410/415 of 1w7mA
- active site: F122 (≠ Y135), D207 (= D212), V209 (= V214), K241 (= K246)
- binding phenylalanine: W15 (≠ F30), G33 (= G48), Y98 (≠ T111), F122 (≠ Y135), N179 (= N184), R392 (= R375)
- binding pyridoxal-5'-phosphate: G96 (= G109), G97 (≠ A110), Y98 (≠ T111), F122 (≠ Y135), N175 (= N180), N179 (= N184), D207 (= D212), V209 (= V214), Y210 (= Y215), S238 (= S243), K241 (= K246), K249 (= K254)
1w7lA Crystal structure of human kynurenine aminotransferase i (see paper)
34% identity, 95% coverage: 19:393/395 of query aligns to 4:410/415 of 1w7lA
- active site: F122 (≠ Y135), D207 (= D212), V209 (= V214), K241 (= K246)
- binding pyridoxal-5'-phosphate: G96 (= G109), G97 (≠ A110), Y98 (≠ T111), F122 (≠ Y135), N175 (= N180), N179 (= N184), D207 (= D212), V209 (= V214), Y210 (= Y215), S238 (= S243), K241 (= K246), K249 (= K254)
5verA Mouse kynurenine aminotransferase iii, re-refinement of the PDB structure 3e2z (see paper)
34% identity, 95% coverage: 19:394/395 of query aligns to 2:408/410 of 5verA
- active site: Y119 (= Y135), D206 (= D212), V208 (= V214), K240 (= K246)
- binding calcium ion: E6 (≠ P23)
- binding pyridoxal-5'-phosphate: G93 (= G109), A94 (= A110), Y95 (≠ T111), N174 (= N180), N178 (= N184), D206 (= D212), Y209 (= Y215), S237 (= S243), K240 (= K246), K248 (= K254)
Sites not aligning to the query:
5vepA Mouse kynurenine aminotransferase iii, re-refinement of the PDB structure 3e2f (see paper)
34% identity, 95% coverage: 19:394/395 of query aligns to 2:408/410 of 5vepA
Sites not aligning to the query:
3e2zA Crystal structure of mouse kynurenine aminotransferase iii in complex with kynurenine (see paper)
34% identity, 95% coverage: 19:394/395 of query aligns to 2:408/410 of 3e2zA
3e2yA Crystal structure of mouse kynurenine aminotransferase iii in complex with glutamine (see paper)
34% identity, 95% coverage: 19:394/395 of query aligns to 2:408/410 of 3e2yA
- active site: Y119 (= Y135), D206 (= D212), V208 (= V214), K240 (= K246)
- binding glutamine: W13 (≠ F30), G31 (= G48), Y119 (= Y135), R389 (= R375)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G93 (= G109), A94 (= A110), Y95 (≠ T111), Y119 (= Y135), N174 (= N180), D206 (= D212), Y209 (= Y215), S237 (= S243), K240 (= K246), K248 (= K254)
3fvuA Crystal structure of human kynurenine aminotransferase i in complex with indole-3-acetic acid (see paper)
33% identity, 95% coverage: 19:393/395 of query aligns to 4:413/419 of 3fvuA
- active site: F122 (≠ Y135), D210 (= D212), V212 (= V214), K244 (= K246)
- binding glycerol: A74 (= A87), S75 (≠ G88), E84 (≠ A97), I85 (≠ Y98)
- binding 1h-indol-3-ylacetic acid: W15 (≠ F30), G33 (= G48), Y60 (= Y73), Y98 (≠ T111), F122 (≠ Y135), K244 (= K246), F275 (= F277), H276 (≠ T278), R395 (= R375)
3fvsB Human kynurenine aminotransferase i in complex with glycerol (see paper)
33% identity, 95% coverage: 19:393/395 of query aligns to 4:413/419 of 3fvsB
Q16773 Kynurenine--oxoglutarate transaminase 1; Cysteine-S-conjugate beta-lyase; Glutamine transaminase K; GTK; Glutamine--phenylpyruvate transaminase; Kynurenine aminotransferase 1; Kynurenine aminotransferase I; KATI; Kynurenine--oxoglutarate transaminase I; EC 2.6.1.7; EC 4.4.1.13; EC 2.6.1.64 from Homo sapiens (Human) (see paper)
33% identity, 95% coverage: 19:393/395 of query aligns to 7:416/422 of Q16773
- G36 (= G48) binding
- N185 (= N184) binding
- K247 (= K246) modified: N6-(pyridoxal phosphate)lysine
- R398 (= R375) binding
1v2fA Crystal structure of t.Th hb8 glutamine aminotransferase complex with 3-phenylpropionate (see paper)
37% identity, 92% coverage: 28:392/395 of query aligns to 13:364/368 of 1v2fA
- active site: F112 (≠ Y135), A181 (≠ L202), A183 (≠ G204), K222 (= K246)
- binding hydrocinnamic acid: F15 (= F30), Q32 (= Q47), G33 (= G48), Y57 (= Y73), F112 (≠ Y135), F253 (= F277), R347 (= R375)
- binding pyridoxal-5'-phosphate: G86 (= G109), A87 (= A110), T88 (= T111), F112 (≠ Y135), N163 (= N184), D191 (= D212), V193 (= V214), Y194 (= Y215), S219 (= S243), K222 (= K246), R230 (≠ K254)
1v2eA Crystal structure of t.Th hb8 glutamine aminotransferase complex with a-keto-g-methylthiobutyrate (see paper)
37% identity, 92% coverage: 28:392/395 of query aligns to 13:364/368 of 1v2eA
- active site: F112 (≠ Y135), A181 (≠ L202), A183 (≠ G204), K222 (= K246)
- binding 4-(methylsulfanyl)-2-oxobutanoic acid: F15 (= F30), Q32 (= Q47), G33 (= G48), F112 (≠ Y135), K222 (= K246), F309 (= F330), R347 (= R375)
- binding pyridoxal-5'-phosphate: G86 (= G109), A87 (= A110), T88 (= T111), F112 (≠ Y135), D191 (= D212), V193 (= V214), Y194 (= Y215), S219 (= S243), K222 (= K246), R230 (≠ K254)
Q17CS8 Kynurenine aminotransferase; AeKAT; EC 2.6.1.-; EC 2.6.1.63; EC 2.6.1.7 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see 2 papers)
33% identity, 91% coverage: 36:393/395 of query aligns to 81:471/477 of Q17CS8
- Y121 (= Y73) binding
- AY 158:159 (≠ AT 110:111) binding in other chain
- N241 (= N184) binding in other chain
- Y272 (= Y215) binding in other chain
- S300 (= S243) binding in other chain
- K303 (= K246) modified: N6-(pyridoxal phosphate)lysine
- K311 (= K254) binding in other chain
- R453 (= R375) binding
Sites not aligning to the query:
2r5eA Aedes kynurenine aminotransferase in complex with glutamine (see paper)
33% identity, 93% coverage: 28:393/395 of query aligns to 15:413/419 of 2r5eA
- active site: F125 (≠ Y135), D211 (= D212), V213 (= V214), K245 (= K246)
- binding n~2~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-glutamine: W17 (≠ F30), Q34 (= Q47), Y63 (= Y73), G99 (= G109), A100 (= A110), Y101 (≠ T111), F125 (≠ Y135), N179 (= N180), N183 (= N184), D211 (= D212), Y214 (= Y215), S242 (= S243), K245 (= K246), K253 (= K254), Y276 (≠ F277), F337 (= F330), R395 (= R375)
2r5cB Aedes kynurenine aminotransferase in complex with cysteine (see paper)
33% identity, 93% coverage: 28:393/395 of query aligns to 15:413/419 of 2r5cB
- active site: F125 (≠ Y135), D211 (= D212), V213 (= V214), K245 (= K246)
- binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: W17 (≠ F30), G35 (= G48), G99 (= G109), A100 (= A110), Y101 (≠ T111), F125 (≠ Y135), N179 (= N180), N183 (= N184), D211 (= D212), Y214 (= Y215), S242 (= S243), K245 (= K246), K253 (= K254), F337 (= F330), R395 (= R375)
1yiyA Aedes aegypti kynurenine aminotransferase (see paper)
33% identity, 93% coverage: 28:393/395 of query aligns to 14:412/418 of 1yiyA
- active site: F124 (≠ Y135), D210 (= D212), V212 (= V214), K244 (= K246)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G98 (= G109), A99 (= A110), Y100 (≠ T111), F124 (≠ Y135), N178 (= N180), D210 (= D212), V212 (= V214), Y213 (= Y215), S241 (= S243), K244 (= K246), K252 (= K254)
1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
31% identity, 90% coverage: 37:393/395 of query aligns to 22:379/388 of 1gdeA
- active site: K232 (= K246)
- binding glutamic acid: F120 (≠ Y135), N170 (= N184), R361 (= R375)
- binding pyridoxal-5'-phosphate: G94 (= G109), A95 (= A110), N96 (≠ T111), F120 (≠ Y135), N166 (= N180), D198 (= D212), Y201 (= Y215), S231 (≠ G245), K232 (= K246), R240 (≠ K254)
Query Sequence
>RR42_RS05770 FitnessBrowser__Cup4G11:RR42_RS05770
MPTNHSAQPGTLAPLTPPASRLPKVGTTIFTVMSALAAERNAVNLGQGFPDFDCDPKIVD
AVAHAMRSGLNQYPPMAGVPKLRQAIAGKIGKLYGHAYDWDSEITVTAGATQGILTTILC
AVHPGDEVIVLEPCYDSYIPAIEMAGGKVVSVALDAPHFHIPFDRLAAAITPRTRMLLIN
TPHNPTGTIWRAGDMDRLAGLLAGTDILLLSDEVYEHMVFDGQPHQSVSRHPELARRSFV
VSSFGKTYHVTGWKVGYVAAPAALSAEFRKVHQFNVFTVNTPVQHGLADYMADPAPYLDL
AAFYQAKRDFFRAGLAATRFKLLPSDGTYFQCVDYSAISDMSEADFSMWLTREIGVAAIP
VSAFYSQPRESGVVRFCFAKKEETLALALARLKAL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory