SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS06325 FitnessBrowser__Cup4G11:RR42_RS06325 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
42% identity, 75% coverage: 1:185/247 of query aligns to 77:261/262 of 1t3dA

query
sites
1t3dA

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binding cysteine: D92 (= D16), P93 (= P17), D157 (= D81), G184 (= G108), R192 (= R117), H193 (= H118)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
45% identity, 68% coverage: 1:169/247 of query aligns to 73:240/258 of 8i04A

query
sites
8i04A

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binding serine: D88 (= D16), P89 (= P17), D153 (= D81), H154 (= H82), G180 (= G108), R188 (= R117), H189 (= H118)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
45% identity, 68% coverage: 1:169/247 of query aligns to 77:244/244 of 8i06A

query
sites
8i06A

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binding coenzyme a: H158 (= H82), L177 (≠ Y101), Q178 (= Q102), G203 (≠ S128), A204 (= A129), G221 (= G146), A222 (≠ S147), T235 (= T160), A237 (≠ V162)
binding cysteine: P93 (= P17), H158 (= H82)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
45% identity, 68% coverage: 1:169/247 of query aligns to 76:243/246 of 8i09A

query
sites
8i09A

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binding cysteine: D91 (= D16), D156 (= D81), H157 (= H82), G164 (= G89), R191 (= R117), H192 (= H118)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G108), T184 (= T109), G202 (≠ S128), K218 (≠ R144), I219 (= I145), G220 (= G146), L226 (= L152), A236 (≠ V162)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
44% identity, 67% coverage: 8:172/247 of query aligns to 80:243/257 of 1ssqD

query
sites
1ssqD

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binding cysteine: D88 (= D16), D153 (= D81), H154 (= H82)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
47% identity, 61% coverage: 5:155/247 of query aligns to 103:252/280 of 7bw9A

query
sites
7bw9A

L

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binding cysteine: D114 (= D16), P115 (= P17), D179 (= D81), H180 (= H82), A206 (≠ G108), R214 (= R117), H215 (= H118)

1sstA Serine acetyltransferase- complex with coa (see paper)
43% identity, 66% coverage: 8:169/247 of query aligns to 80:233/233 of 1sstA

query
sites
1sstA

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x
V

binding coenzyme a: H154 (= H82), L173 (≠ Y101), G180 (= G108), T181 (= T109), A193 (= A129), L198 (= L134), K208 (≠ R144), G210 (= G146), A211 (≠ S147), V214 (= V150), L216 (= L152), T224 (= T160), A226 (≠ V162), V228 (≠ I164), P229 (= P165), V233 (≠ I169)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
42% identity, 68% coverage: 8:175/247 of query aligns to 80:249/258 of 4h7oA

query
sites
4h7oA

D

D

I

I

D

C

A

A

I

T

M

V

R

N

R

R

D
|
D

P
|
P

A

A

A

V

R

S

S

M

R

Y

L

S

E

M

V

P

L

L

T

L

C

Y

Y

L

P

K

G

G

F

Y

H

H

A

A

V

L

V

Q

F

G

H

Y

R

R

F

V

A

A

H

N

A

W

C

L

W

W

N

R

A

Q

G

G

F

R

H

K

W

A

L

L

G

A

R

T

W
x
Y

I

F

S

Q

H

N

W
x
Q

A

I

R

S

F

V

F

A

T

C

G

Q

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

V

I

G

G

R

R

R

G

V

I

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

I

V

V

V

I

G

G

E

E

T

T

A

A

E

V

I

V

G

E

D

D

D

D

C

V

T

S

I

I

Y

L

Q

Q

G

D

V

V

T

T

L

L

G

G

G
|
G

T

T

S

G

L

K

Y

E

K

C

G

G

V

-

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

R

A

E

N

G

V

V

V

M

V

I

S

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

G

N

Y

I

T

E

I

V

G

G

D

E

G

G

A

A

R

K

I

I

G

G

S

S

N

G

A

S

V

V

V

V

L

L

K

Q

P

A

V

V

P

P

P

P

G

H

A

T

T

T

A

V

V

A

G

G

I

V

P

P

A

A

R

R

I

I

I

V

-

G

-

R

-

P

L

Q

P

S

D

D

A

K

P

P

S

S

binding arginine: Y127 (≠ W55), Q131 (≠ W59)
binding cysteine: D88 (= D16), P89 (= P17), D153 (= D81), H154 (= H82), G180 (= G108), R188 (= R117), H189 (= H118)

Sites not aligning to the query:

binding arginine: 51, 53

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
43% identity, 64% coverage: 5:161/247 of query aligns to 105:268/270 of 3p47A

query
sites
3p47A

L

L

K

K

E

T

D

D

I

L

D

I

A

A

I

A

M

Y

R

A

R

G

D

D

P

P

A

A

A

A

R

P

S

G

R

L

L

S

E

L

V

I

L

I

T

R

C

C

Y

Y

P

P

G

G

F

F

H

Q

A

A

V

V

V

I

F

V

H

Y

R

R

F

I

A

A

H

H

A

V

C

L

W

Y

N

E

A

C

G

G

F

E

H

R

W

Y

L

Y

G

C

R

R

W

E

I

M

S

M

H

E

W

S

A

V

R

H

F

S

F

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

K

S

V

I

G

K

R

G

R

H

V

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

V

I

G

G

E

E

T

T

A

A

E

I

I

I

G

G

D

E

D

W

C

C

T

R

I

I

Y

Y

Q

Q

G

S

V

V

T

T

L

L

G

G

-
x
A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

T

G

S

V

L

I

Y

K

K

R

G

G

V

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

G

G

A

D

N

Y

V

V

V

T

V

I

S

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

G

N

Y

I

T

I

I

V

G

G

D

S

G

H

A

V

R

R

I

I

G

G

S

A

N

N

A

C

V

W

V

I

L

D

K

R

P

D

V

V

P

D

P

S

G

N

A

Q

T

T

A

V

binding cysteine: A208 (vs. gap), H210 (vs. gap), R224 (= R117), H225 (= H118)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
43% identity, 64% coverage: 5:161/247 of query aligns to 103:266/267 of 3q1xA

query
sites
3q1xA

L

L

K

K

E

T

D

D

I

L

D

I

A

A

I

A

M

Y

R

A

R

G

D

D

P

P

A

A

A

A

R

P

S

G

R

L

L

S

E

L

V

I

L

I

T

R

C

C

Y

Y

P

P

G

G

F

F

H

Q

A

A

V

V

V

I

F

V

H

Y

R

R

F

I

A

A

H

H

A

V

C

L

W

Y

N

E

A

C

G

G

F

E

H

R

W

Y

L

Y

G

C

R

R

W

E

I

M

S

M

H

E

W

S

A

V

R

H

F

S

F

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

K

S

V

I

G

K

R

G

R

H

V

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

V

I

G

G

E

E

T

T

A

A

E

I

I

I

G

G

D

E

D

W

C

C

T

R

I

I

Y

Y

Q

Q

G

S

V

V

T

T

L

L

G

G

-

A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

T

G

S

V

L

I

Y

K

K

R

G

G

V

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

G

G

A

D

N

Y

V

V

V

T

V

I

S

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

G

N

Y

I

T

I

I

V

G

G

D

S

G

H

A

V

R

R

I

I

G

G

S

A

N

N

A

C

V

W

V

I

L

D

K

R

P

D

V

V

P

D

P

S

G

N

A

Q

T

T

A

V

binding serine: H208 (vs. gap), R222 (= R117), H223 (= H118)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
40% identity, 68% coverage: 1:169/247 of query aligns to 80:247/272 of 3gvdI

query
sites
3gvdI

M

M

F

I

T

V

R

S

L

A

K

A

E

R

D

D

I

I

D

L

A

A

I

V

M

R

R

L

R

R

D
|
D

P
|
P

A

A

A

V

R

D

S

K

R

Y

L

S

E

T

V

P

L

L

T

L

C

Y

Y

L

P

K

G

G

F

F

H

H

A

A

V

L

V

Q

F

A

H

Y

R

R

F

I

A

G

H

H

A

W

C

L

W

W

N

A

A

Q

G

D

F

R

H

K

W

A

L

L

G

A

R

I

W

Y

I

L

S

Q

H

N

W

Q

A

V

R

S

F

V

F

A

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

T

V

I

G

G

R

C

R

G

V

I

F

M

I

L

D
|
D

H

H

G

A

M

T

G

G

V

I

V

V

V

I

G

G

E

E

T

T

A

A

E

V

I

V

G

E

D

N

D

D

C

V

T

S

I

I

Y

L

Q

Q

G

S

V

V

T

T

L

L

G
|
G

G
|
G

T

T

S

G

L

K

Y

T

K

S

G

G

V

-

K

D

R
|
R

H

H

P

P

T

K

L

I

G

R

A

E

N

G

V

V

V

M

V

I

S

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

G

N

Y

I

T

E

I

V

G

G

D

R

G

G

A

A

R

K

I

I

G

G

S

A

N

G

A

S

V

V

V

V

L

L

K

Q

P

S

V

V

P

P

P

A

G

H

A

T

T

T

A

A

V

A

G

G

I

V

P

P

A

A

R

R

I

I

I

V

binding cysteine: D95 (= D16), P96 (= P17), D160 (= D81), G186 (= G107), G187 (= G108), R195 (= R117)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
41% identity, 67% coverage: 5:169/247 of query aligns to 81:244/250 of 4hzdA

query
sites
4hzdA

L

L

K

R

E

V

D

D

I

I

D

Q

A

A

I

V

M

Y

R

D

R

R

D

D

P

P

A

A

A

Y

R

S

S

R

R

F

L

M

E

D

V

P

L

V

T

L

C

Y

Y

L

P

K

G

G

F

F

H

H

A

A

V

I

V

Q

F

T

H

H

R

R

F

L

A

A

H

H

A

W

C

L

W

Y

N

K

A

Q

G

G

F

R

H

K

W

D

L

F

G

A

R

Y

W

Y

I

L

S

Q

H

S

W

R

A

S

R

S

F

S

F

I

T

F

G

Q

I

T

E

D

I

I

H

H

P

P

G

A

A

A

K

R

V

L

G

G

R

S

R

G

V

L

F

F

I

L

D

D

H
|
H

G

A

M

T

G

G

V

L

V

V

V

V

G

G

E

E

T

T

A

A

E

V

I

V

G

E

D

D

D

N

C

V

T

S

I

I

Y

L

Q

H

G

G

V

V

T

T

L

L

G

G

G

G

T

T

S

G

L

K

Y

S

K

S

G

G

V

-

K

D

R

R

H

H

P

P

T

K

L

I

G

R

A

Q

N

G

V

V

V

L

V

I

S
x
G

A
|
A

G

G

A

A

K

K

V

I

L

L

G

G

G

N

Y

I

T

Q

I

V

G

G

D

Q

G

C

A

S

R

K

I

I

G
x
A

S
x
A

N

G

A
x
S

V

V

V

V

L

L

K

K

P

S

V

V

P

P

P

H

G

N

A

V

T
|
T

A

V

V
x
A

G
|
G

I

V

P

P

A

A

R

R

I

I

I
|
I

binding coenzyme a: H158 (= H82), G203 (≠ S128), A204 (= A129), A221 (≠ G146), A222 (≠ S147), T235 (= T160), A237 (≠ V162), I244 (= I169)
binding magnesium ion: S224 (≠ A149), A237 (≠ V162), G238 (= G163)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
45% identity, 66% coverage: 8:169/247 of query aligns to 83:243/243 of 4n69A

query
sites
4n69A

D

D

I

L

D

R

A

A

I

A

M

R

R

E

R

R

D

D

P

P

A

A

A

C

R

V

S

S

R

Y

L

S

E

H

V

C

L

L

T

L

C

N

Y

Y

P

K

G

G

F

F

H

L

A

A

V

C

V

Q

F

A

H

H

R

R

F

V

A

A

H

H

A

L

C

L

W

W

N

R

A

Q

G

S

F

R

H

R

W

P

L

L

G

A

R

L

W

A

I

L

-

H

S

S

H

R

W

I

A

A

R

N

F

V

F

F

T

A

G

-

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

V

I

G

G

R

K

R

G

V

I

F

L

I

F

D

D

H

H

G

A

M

T

G

G

V

V

V

V

V

V

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

D

N

C

V

T

S

I

I

Y

L

Q

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

S

G

L

K

Y

V

K

G

G

G

V

-

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

G

A

D

N

G

V

V

V

L

V

I

S

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

G

N

Y

I

T

K

I

I

G

G

D

E

G

G

A

A

R

K

I

V

G

G

S

A

N

G

A

S

V

V

V

V

L

L

K

I

P

D

V

V

P

P

P

P

G

R

A

T

T

T

A

A

V

V

G

G

I

N

P

P

A

A

R

R

I

L

I

V

binding serine: G183 (= G108), R191 (= R117), H192 (= H118)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
39% identity, 66% coverage: 5:167/247 of query aligns to 82:243/261 of 6wyeA

query
sites
6wyeA

L

V

K

E

E

A

D

D

I

L

D

K

A

A

I

I

M

Y

R

E

R

R

D
|
D

P
|
P

A

A

A

C

R

D

S

E

R

Y

L

S

E

L

V

P

L

L

T

L

C

Y

Y

F

P

K

G

G

F

F

H

H

A

A

V

I

V

Q

F

A

H

H

R

R

F

I

A

N

H

H

A

R

C

L

W

Y

N

L

A

D

G

G

F

R

H

K

W

T

L

L

G

A

R

Y

W

F

I

L

S

Q

H

N

W

R

A

M

R

S

F

E

F

V

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

V

L

G

G

R

Y

R

G

V

L

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

F

V

V

V

A

G

G

E

E

T

T

A

A

E

V

I

L

G

G

D

N

D

N

C

I

T

S

I

I

Y

L

Q

H

G

G

V

V

T

T

L

L

G

G

G
|
G

T

S

S

G

L

-

Y

K

K

E

G

G

V

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

G

A

D

N

G

V

V

V

M

V

I

S

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

G

N

Y

I

T

R

I

I

G

G

D

S

G

N

A

A

R

K

I

I

G

G

S

A

N

G

A

S

V

V

V

V

L

V

K

S

P

D

V

V

P

P

P

P

G

S

A

I

T

T

A

V

V

V

G

G

I

V

P

P

A

A

R

K

binding (2S)-2-hydroxybutanedioic acid: D93 (= D16), P94 (= P17), D158 (= D81), H159 (= H82), G185 (= G108), R193 (= R117), H194 (= H118)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
39% identity, 66% coverage: 5:167/247 of query aligns to 80:241/243 of 7ra4A

query
sites
7ra4A

L

V

K

E

E

A

D

D

I

L

D

K

A

A

I

I

M

Y

R

E

R

R

D
|
D

P
|
P

A

A

A

C

R

D

S

E

R

Y

L

S

E

L

V

P

L

L

T

L

C

Y

Y

F

P

K

G

G

F

F

H

H

A

A

V

I

V

Q

F

A

H

H

R

R

F

I

A

N

H

H

A

R

C

L

W

Y

N

L

A

D

G

G

F

R

H

K

W

T

L

L

G

A

R

Y

W

F

I

L

S

Q

H

N

W

R

A

M

R

S

F

E

F

V

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

V

L

G

G

R

Y

R

G

V

L

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

F

V

V

V

A

G

G

E

E

T

T

A

A

E

V

I

L

G

G

D

N

D

N

C

I

T

S

I

I

Y

L

Q

H

G

G

V

V

T

T

L

L

G

G

G

G

T

S

S

G

L

-

Y

K

K

E

G

G

V

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

G

A

D

N

G

V

V

V

M

V

I

S

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

G

N

Y

I

T

R

I

I

G

G

D

S

G

N

A

A

R

K

I

I

G

G

S

A

N

G

A

S

V

V

V

V

L

V

K

S

P

D

V

V

P

P

P

P

G

S

A

I

T

T

A

V

V

V

G

G

I

V

P

P

A

A

R

K

binding serine: D91 (= D16), P92 (= P17), D156 (= D81), H157 (= H82), R191 (= R117), H192 (= H118)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
44% identity, 66% coverage: 8:169/247 of query aligns to 79:233/233 of 4n6bA

query
sites
4n6bA

D

D

I

L

D

R

A

A

I

A

M

R

R

E

R

R

D

D

P

V

A

-

A

-

R

-

S

S

R

Y

L

S

E

H

V

C

L

L

T

L

C

N

Y

Y

P

K

G

G

F

F

H

L

A

A

V

C

V

Q

F

A

H

H

R

R

F

V

A

A

H

H

A

L

C

L

W

W

N

R

A

Q

G

S

F

R

H

R

W

P

L

L

G

A

R

L

W

A

I

L

-

H

S

S

H

R

W

I

A

A

R

N

F

V

F

F

T

-

G

A

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

V

I

G

G

R

K

R

G

V

I

F

L

I

F

D

D

H
|
H

G

A

M

T

G

G

V

V

V

V

V

V

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

D

N

C

V

T

S

I

I

Y
x
L

Q

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

S

-

L

-

Y

-

K

-

G

G

V

G

K

D

R

R

H

H

P

P

T

K

L

I

G

G

A

D

N

G

V

V

V

L

V

I

S

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

G

N

Y

I

T

K

I

I

G

G

D

E

G

G

A

A

R
x
K

I

V

G
|
G

S
x
A

N

G

A

S

V

V

V

V

L
|
L

K

I

P

D

V

V

P

P

P

P

G

R

A

T

T

T

A

A

V
|
V

G

G

I

N

P

P

A

A

R

R

I

L

I

V

binding coenzyme a: H150 (= H82), L169 (≠ Y101), G176 (= G108), K208 (≠ R144), G210 (= G146), A211 (≠ S147), L216 (= L152), V226 (= V162)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
31% identity, 47% coverage: 55:169/247 of query aligns to 62:181/201 of 1krvA

query
sites
1krvA

W

W

I

V

S

E

H

P

W

P

A

V

R

Y

F

F

F
x
S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

K

-

V

I

G

G

R

R

R

N

V

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V
|
V

V

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

C

V

T

L

I

I

Y
x
A

Q

P

G

N

V

V

T

T

L

L

G
x
S

G

V

T
|
T

-

G

-

H

-

P

-

V

-

H

-

H

S

E

L

L

Y

R

K

K

G

N

V

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

A

N

N

N

V

V

V
x
W

V

I

S
x
G

A
x
S

G

H

A

V

K

V

V

I

L
x
N

G

P

G

G

Y

V

T

T

I

I

G

G

D

D

G

N

A

S

R

V

I

I

G

G

S
x
A

N

G

A

S

V

I

V

V

L

T

K

K

P

D

V

I

P

P

P

P

G

N

A

V

T

V

A

A

V

A

G

G

I

V

P
|
P

A

C

R
|
R

I

V

I

I

binding 4-nitrophenyl beta-D-galactopyranoside: S70 (≠ F63), Y82 (≠ F79), N84 (≠ D81), V90 (= V87), D92 (≠ G89), M126 (≠ R117)
binding coenzyme a: A104 (≠ Y101), S110 (≠ G107), T112 (= T109), W138 (≠ V126), G140 (≠ S128), S141 (≠ A129), N146 (≠ L134), A159 (≠ S147), P177 (= P165), R179 (= R167)

Sites not aligning to the query:

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
31% identity, 47% coverage: 55:169/247 of query aligns to 62:181/201 of 1kruA

query
sites
1kruA

W
|
W

I

V

S

E

H

P

W

P

A

V

R

Y

F

F

F
x
S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

K

-

V

I

G

G

R

R

R

N

V

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V
|
V

V

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

C

V

T
x
L

I

I

Y

A

Q

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

-

G

-
x
H

-

P

-

V

-

H

-

H

S

E

L

L

Y

R

K

K

G

N

V

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

A

N

N

N

V

V

V
x
W

V

I

S
x
G

A
x
S

G

H

A

V

K

V

V

I

L

N

G

P

G

G

Y

V

T

T

I

I

G

G

D

D

G

N

A

S

R

V

I

I

G
|
G

S
x
A

N

G

A

S

V

I

V

V

L

T

K

K

P

D

V

I

P

P

P

P

G

N

A

V

T

V

A

A

V

A

G

G

I

V

P
|
P

A

C

R

R

I

V

I

I

binding coenzyme a: H114 (vs. gap), W138 (≠ V126), G140 (≠ S128), S141 (≠ A129), G158 (= G146), A159 (≠ S147), P177 (= P165)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: W62 (= W55), S70 (≠ F63), Y82 (≠ F79), Y82 (≠ F79), N84 (≠ D81), V90 (= V87), D92 (≠ G89), L102 (≠ T99), H114 (vs. gap), M126 (≠ R117)

Sites not aligning to the query:

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 47% coverage: 55:169/247 of query aligns to 63:182/203 of P07464

query
sites
P07464

W

W

I

V

S

E

H

P

W

P

A

V

R

Y

F

F

F
x
S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

K

-

V

I

G

G

R

R

R

N

V

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

V

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

C

V

T

L

I

I

Y

A

Q

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

-

G

-
x
H

-

P

-

V

-

H

-

H

S

E

L

L

Y

R

K

K

G

N

V

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

A

N

N

N

V

V

V

W

V

I

S

G

A
x
S

G

H

A

V

K

V

V

I

L

N

G

P

G

G

Y

V

T

T

I

I

G

G

D

D

G

N

A

S

R

V

I

I

G

G

S
x
A

N

G

A

S

V

I

V

V

L
x
T

K
|
K

P

D

V

I

P

P

P

P

G

N

A

V

T

V

A

A

V

A

G

G

I

V

P

P

A

C

R
|
R

I

V

I

I

S71 (≠ F63) binding
N85 (≠ D81) binding in other chain; binding in other chain
D93 (≠ G89) binding
H115 (vs. gap) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A129) binding in other chain
A160 (≠ S147) binding in other chain
TK 165:166 (≠ LK 152:153) binding
R180 (= R167) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
183 binding in other chain

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
31% identity, 47% coverage: 55:169/247 of query aligns to 62:181/200 of 1krrA

query
sites
1krrA

W

W

I

V

S

E

H

P

W

P

A

V

R

Y

F

F

F

S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

K

-

V

I

G

G

R

R

R

N

V

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

V

D

G

D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

C

V

T

L

I
|
I

Y
x
A

Q
x
P

G

N

V

V

T

T

L

L

G

S

G

V

T
|
T

-

G

-
x
H

-

P

-

V

-

H

-

H

S

E

L

L

Y

R

K

K

G

N

V

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

A

N

N

N

V

V

V

W

V

I

S
x
G

A
x
S

G

H

A

V

K

V

V

I

L
x
N

G

P

G

G

Y

V

T

T

I

I

G

G

D

D

G

N

A

S

R

V

I

I

G
|
G

S
x
A

N

G

A

S

V

I

V

V

L

T

K

K

P

D

V

I

P

P

P

P

G

N

A

V

T

V

A

A

V
x
A

G

G

I

V

P
|
P

A

C

R

R

I

V

I

I

binding acetyl coenzyme *a: N84 (≠ D81), I103 (= I100), A104 (≠ Y101), P105 (≠ Q102), T112 (= T109), H114 (vs. gap), G140 (≠ S128), S141 (≠ A129), N146 (≠ L134), G158 (= G146), A159 (≠ S147), A174 (≠ V162), P177 (= P165)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

Query Sequence

>RR42_RS06325 FitnessBrowser__Cup4G11:RR42_RS06325
MFTRLKEDIDAIMRRDPAARSRLEVLTCYPGFHAVVFHRFAHACWNAGFHWLGRWISHWA
RFFTGIEIHPGAKVGRRVFIDHGMGVVVGETAEIGDDCTIYQGVTLGGTSLYKGVKRHPT
LGANVVVSAGAKVLGGYTIGDGARIGSNAVVLKPVPPGATAVGIPARIILPDAPSGQQGA
KQEFSAYGITPNADDPVSLALKSLIDNAAQQHERLETVMAALDRLGEHLENTPNDRFDAS
ELRKLMK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory