SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS07660 FitnessBrowser__Cup4G11:RR42_RS07660 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
41% identity, 100% coverage: 1:283/284 of query aligns to 110:385/409 of O53289

query
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O53289

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D185 (= D81) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ M82) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D83) mutation to N: Decreases enzymatic activity by 15%.
S188 (= S84) mutation to A: No effect on enzymatic activity.
S273 (= S171) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K216) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D239) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D243) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

7qplA Crystal structure of phosphoserine phosphatase (serb) from brucella melitensis in complex with phosphate and magnesium
45% identity, 74% coverage: 76:284/284 of query aligns to 81:288/295 of 7qplA

query
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7qplA

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binding magnesium ion: D86 (= D81), D88 (= D83), D243 (= D239)

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
41% identity, 95% coverage: 15:283/284 of query aligns to 121:383/396 of 5jlpA

query
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5jlpA

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active site: D183 (= D81), V184 (≠ M82), D185 (= D83), G272 (= G172), K316 (= K216), D343 (= D243)
binding magnesium ion: D183 (= D81), D339 (= D239), D343 (= D243)

Sites not aligning to the query:

binding serine: 13, 14, 18, 37

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
41% identity, 95% coverage: 15:283/284 of query aligns to 125:387/411 of A0QJI1

query
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A0QJI1

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D187 (= D81) binding
D189 (= D83) binding
D343 (= D239) binding

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
41% identity, 95% coverage: 15:283/284 of query aligns to 121:383/396 of 8a21A

query
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8a21A

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S

S

G
|
G

F

F

V

R

H

R

F

I

T

I

D

E

K

P

L

L

K

A

P

E

R

E

L

L

K

M

L

L

D

D

V

Y

T

V

R

A

A

A

N

N

T

E

L

L

E

E

I

I

V

V

D

D

G

G

K

T

L

L

T

T

G

G

Q

R

V

V

L

V

G

G

E

P

I

I

V

I

N

D

A

R

D

A

V

G

K

K

A

A

R

T

T

A

V

L

Q

R

E

E

V

F

C

A

A

Q

Q

R

I

A

G

G

A

V

D

P

P

M

S

A

Q

Q

A

T

I

V

V

A

M

V

G

G

D
|
D

G

G

S

A

N

N

D

D

L

I

K

D

M

M

M

L

A

A

V

A

A

A

G

G

L

L

S

G

V

I

A

A

F

F

R

N

A

A

K

K

P

P

V

A

V

L

R

R

A

E

Q

V

A

A

S

D

V

A

A

S

F

L

N

S

H

H

V

P

G

Y

L

L

D

D

G

T

L

V

L

L

Q

F

L

L

F

L

A

G

binding magnesium ion: D183 (= D81), D185 (= D83), D339 (= D239)
binding 4-phenyl-1h-imidazole: D185 (= D83), E192 (= E90), V193 (≠ C91), I194 (= I92), T211 (= T109), M215 (= M113), F221 (= F120), R228 (= R127), G272 (= G172), G273 (= G173)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
41% identity, 95% coverage: 15:283/284 of query aligns to 121:383/396 of 8a1zA

query
sites
8a1zA

D

N

I

I

D

D

A

L

V

I

R

R

A

G

I

V

A

S

Q

D

A

Y

P

P

T

V

-

I

-

G

L

L

A

E

P

L

R

R

A

V

E

S

T

V

V

P

A

P

A

G

A

A

D

D

A

E

C

-

A

-

L

-

T

-

P

-

A

A

L

L

R

R

E

T

A

A

L

L

D

N

A

R

Y

V

C

S

G

S

P

E

R

E

S

H

I

V

D

D

W

V

A

A

I

V

V

E

P

D

A

Y

G

T

R

L

K

E

L

R

S

R

D

A

F

K

K

R

L

L

V

I

A

V

M

F

D
|
D

M

V

D
|
D

S
|
S

T

T

L

L

I

V

T

Q

I

G

E
|
E

C

V

I
|
I

D

E

E

M

I

L

A

A

D

A

F

K

C

A

G

G

L

A

K

E

A

G

E

Q

V

V

S

A

A

A

I

I

T

T

E

D

A

A

M
|
M

R

R

G

G

E

E

I

L

T

-

D

D

F
|
F

N

A

E

Q

S

S

L
|
L

R

Q

R
|
R

V

V

A

A

L

T

L

L

K

A

G

G

L

L

D

P

A

A

S

T

V

V

L

I

D

D

R

E

V

V

Y

A

E

G

E

Q

R

-

L

L

R

E

L

L

S

M

P

P

G

G

A

A

E

R

T

T

M

T

L

L

R

R

T

T

V

L

Q

R

A

R

L

L

G

G

M

Y

R

A

T

C

L

G

L

V

V

V

S

S

G
|
G

F
|
F

V

R

H

R

F

I

T

I

D

E

K

P

L

L

K

A

P

E

R

E

L

L

K

M

L

L

D

D

V

Y

T

V

R

A

A

A

N

N

T

E

L

L

E

E

I

I

V

V

D

D

G

G

K

T

L

L

T

T

G

G

Q

R

V

V

L

V

G

G

E

P

I

I

V

I

N

D

A

R

D

A

V

G

K

K

A

A

R

T

T

A

V

L

Q

R

E

E

V

F

C

A

A

Q

Q

R

I

A

G

G

A

V

D

P

P

M

S

A

Q

Q

A

T

I

V

V

A

M

V

G

G

D
|
D

G

G

S

A

N

N

D

D

L

I

K

D

M

M

M

L

A

A

V

A

A

A

G

G

L

L

S

G

V

I

A

A

F

F

R

N

A

A

K

K

P

P

V

A

V

L

R

R

A

E

Q

V

A

A

S

D

V

A

A

S

F

L

N

S

H

H

V

P

G

Y

L

L

D

D

G

T

L

V

L

L

Q

F

L

L

F

L

A

G

binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D183 (= D81), D185 (= D83), S186 (= S84), E192 (= E90), I194 (= I92), M215 (= M113), F221 (= F120), L225 (= L124), R228 (= R127), G272 (= G172), G273 (= G173), F274 (= F174), D339 (= D239)
binding magnesium ion: D183 (= D81), D185 (= D83), D339 (= D239)

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
38% identity, 72% coverage: 76:280/284 of query aligns to 6:208/211 of Q58989

query
sites
Q58989

K

K

L

L

V

I

A

L

M

F

D
|
D

M

F

D
|
D

S

S

T

T

L

L

I

V

T

N

I

N

E
|
E

C

T

I

I

D

D

E

E

I

I

A

A

D

R

F

E

C

A

G

G

L

V

K

E

A

E

E

E

V

V

S

K

A

K

I

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

I

L

T

-

D

N

F

F

N

E

E

Q

S

S

L

L

R

R

R

K

R
|
R

V

V

A

S

L

L

K

K

G

D

L

L

D

P

A

I

S

E

V

K

L

V

D

E

R

K

V

A

Y

I

E

K

E

-

R

R

L

I

R

T

L

P

S

T

P

E

G

G

A

A

E

E

T

E

M

T

L

I

R

K

T

E

V

L

Q

K

A

N

L

R

G

G

M

Y

R

V

T

V

L

A

L

V

V

V

S
|
S

G
|
G

G

G

F

F

V

D

H

I

F

A

T

V

D

N

K

K

L

I

K

K

P

E

R

K

L

L

K

G

L

L

D

D

V

Y

T

A

R

F

A

A

N

N

T

R

L

L

E

I

I

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

Q

D

V

V

L

E

G

G

E

E

I

V

V

L

N

K

A

E

D

N

V

A

K
|
K

A

G

R

E

T

I

V

L

Q

E

E

K

V

I

C

A

A

K

Q

I

I

E

G

G

A

I

D

N

P

L

S

E

Q

D

A

T

I

V

V

A

M

V

G

G

D
|
D

G

G

S

A

N
|
N

D

D

L

I

K

S

M

M

M

F

A

K

V

K

A

A

G

G

L

L

S

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

V

I

V

L

R

K

A

E

Q

K

A

A

S

D

V

I

A

C

F

I

N

E

H

K

V

R

G

D

L

L

D

R

G

E

L

I

L

L

Q

K

D11 (= D81) active site, Nucleophile; binding ; mutation to N: Loss of activity.
D13 (= D83) active site, Proton donor; binding
E20 (= E90) binding
R56 (= R127) binding
SG 99:100 (= SG 171:172) binding
K144 (= K216) binding
D167 (= D239) binding
N170 (= N242) binding

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
38% identity, 72% coverage: 76:280/284 of query aligns to 4:206/209 of 1l7nA

query
sites
1l7nA

K

K

L

L

V

I

A

L

M

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

T

N

I

N

E

E

C

T

I

I

D

D

E

E

I

I

A

A

D

R

F

E

C

A

G

G

L

V

K

E

A

E

E

E

V

V

S

K

A

K

I

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

I

L

T

-

D

N

F

F

N

E

E

Q

S

S

L

L

R

R

R

K

R

R

V

V

A

S

L

L

K

K

G

D

L

L

D

P

A

I

S

E

V

K

L

V

D

E

R

K

V

A

Y

I

E

K

E

-

R

R

L

I

R

T

L

P

S

T

P

E

G

G

A

A

E

E

T

E

M

T

L

I

R

K

T

E

V

L

Q

K

A

N

L

R

G

G

M

Y

R

V

T

V

L

A

L

V

V

V

S
|
S

G
|
G

G

G

F

F

V

D

H

I

F

A

T

V

D

N

K

K

L

I

K

K

P

E

R

K

L

L

K

G

L

L

D

D

V

Y

T

A

R

F

A

A

N

N

T

R

L

L

E

I

I

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

Q

D

V

V

L

E

G

G

E

E

I

V

V

L

N

K

A

E

D

N

V

A

K
|
K

A

G

R

E

T

I

V

L

Q

E

E

K

V

I

C

A

A

K

Q

I

I

E

G

G

A

I

D

N

P

L

S

E

Q

D

A

T

I

V

V

A

M

V

G

G

D
|
D

G

G

S

A

N
|
N

D
|
D

L

I

K

S

M

M

M

F

A

K

V

K

A

A

G

G

L

L

S

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

V

I

V

L

R

K

A

E

Q

K

A

A

S

D

V

I

A

C

F

I

N

E

H

K

V

R

G

D

L

L

D

R

G

E

L

I

L

L

Q

K

active site: D9 (= D81), F10 (≠ M82), D11 (= D83), G98 (= G172), K142 (= K216), D169 (= D243)
binding aluminum fluoride: D9 (= D81), F10 (≠ M82), D11 (= D83), S97 (= S171), G98 (= G172), K142 (= K216), N168 (= N242)
binding tetrafluoroaluminate ion: D9 (= D81), F10 (≠ M82), D11 (= D83), S97 (= S171), G98 (= G172), K142 (= K216), N168 (= N242)
binding magnesium ion: D9 (= D81), D11 (= D83), D165 (= D239)

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
38% identity, 72% coverage: 76:280/284 of query aligns to 5:207/210 of 1f5sA

query
sites
1f5sA

K

K

L

L

V

I

A

L

M

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

T

N

I

N

E

E

C

T

I

I

D

D

E

E

I

I

A

A

D

R

F

E

C

A

G

G

L

V

K

E

A

E

E

E

V

V

S

K

A

K

I

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

I

L

T

-

D

N

F

F

N

E

E

Q

S

S

L

L

R

R

R

K

R

R

V

V

A

S

L

L

K

K

G

D

L

L

D

P

A

I

S

E

V

K

L

V

D

E

R

K

V

A

Y

I

E

K

E

-

R

R

L

I

R

T

L

P

S

T

P

E

G

G

A

A

E

E

T

E

M

T

L

I

R

K

T

E

V

L

Q

K

A

N

L

R

G

G

M

Y

R

V

T

V

L

A

L

V

V

V

S
|
S

G
|
G

F

F

V

D

H

I

F

A

T

V

D

N

K

K

L

I

K

K

P

E

R

K

L

L

K

G

L

L

D

D

V

Y

T

A

R

F

A

A

N

N

T

R

L

L

E

I

I

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

Q

D

V

V

L

E

G

G

E

E

I

V

V

L

N

K

A

E

D

N

V

A

K
|
K

A

G

R

E

T

I

V

L

Q

E

E

K

V

I

C

A

A

K

Q

I

I

E

G

G

A

I

D

N

P

L

S

E

Q

D

A

T

I

V

V

A

M

V

G

G

D
|
D

G

G

S

A

N
|
N

D
|
D

L

I

K

S

M

M

M

F

A

K

V

K

A

A

G

G

L

L

S

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

V

I

V

L

R

K

A

E

Q

K

A

A

S

D

V

I

A

C

F

I

N

E

H

K

V

R

G

D

L

L

D

R

G

E

L

I

L

L

Q

K

active site: D10 (= D81), F11 (≠ M82), D12 (= D83), G99 (= G172), K143 (= K216), D170 (= D243)
binding magnesium ion: D10 (= D81), D12 (= D83), D166 (= D239)
binding phosphate ion: D10 (= D81), F11 (≠ M82), D12 (= D83), S98 (= S171), G99 (= G172), G100 (= G173), K143 (= K216), N169 (= N242)

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
37% identity, 72% coverage: 76:280/284 of query aligns to 3:205/208 of 1l7pA

query
sites
1l7pA

K

K

L

L

V

I

A

L

M

F

D
x
N

M
x
F

D
|
D

S

S

T

T

L

L

I

V

T

N

I

N

E
|
E

C

T

I

I

D

D

E

E

I

I

A

A

D

R

F

E

C

A

G

G

L

V

K

E

A

E

E

E

V

V

S

K

A

K

I

I

T

T

E

K

A

E

A

A

M
|
M

R

E

G

G

E

K

I

L

T

-

D

N

F
|
F

N

E

E

Q

S

S

L

L

R

R

R

K

R
|
R

V

V

A

S

L

L

K

K

G

D

L

L

D

P

A

I

S

E

V

K

L

V

D

E

R

K

V

A

Y

I

E

K

E

-

R

R

L

I

R

T

L

P

S

T

P

E

G

G

A

A

E

E

T

E

M

T

L

I

R

K

T

E

V

L

Q

K

A

N

L

R

G

G

M

Y

R

V

T

V

L

A

L

V

V

V

S
|
S

G
|
G

F

F

V

D

H

I

F

A

T

V

D

N

K

K

L

I

K

K

P

E

R

K

L

L

K

G

L

L

D

D

V

Y

T

A

R

F

A

A

N

N

T

R

L

L

E

I

I

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

Q

D

V

V

L

E

G

G

E

E

I

V

V

L

N

K

A

E

D

N

V

A

K
|
K

A

G

R

E

T

I

V

L

Q

E

E

K

V

I

C

A

A

K

Q

I

I

E

G

G

A

I

D

N

P

L

S

E

Q

D

A

T

I

V

V

A

M

V

G

G

D

D

G

G

S

A

N
|
N

D
|
D

L

I

K

S

M

M

M

F

A

K

V

K

A

A

G

G

L

L

S

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

V

I

V

L

R

K

A

E

Q

K

A

A

S

D

V

I

A

C

F

I

N

E

H

K

V

R

G

D

L

L

D

R

G

E

L

I

L

L

Q

K

active site: N8 (≠ D81), F9 (≠ M82), D10 (= D83), G97 (= G172), K141 (= K216), D168 (= D243)
binding phosphoserine: N8 (≠ D81), F9 (≠ M82), D10 (= D83), E17 (= E90), M40 (= M113), F46 (= F120), R53 (= R127), S96 (= S171), G97 (= G172), G98 (= G173), K141 (= K216), N167 (= N242)

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
36% identity, 72% coverage: 76:280/284 of query aligns to 3:197/200 of 1l7oA

query
sites
1l7oA

K

K

L

L

V

I

A

L

M

F

D

N

M

F

D

D

S

S

T

T

L

L

I

V

T

N

I

N

E

E

C

T

I

I

D

D

E

E

I

I

A

A

D

R

F

E

C

A

G

G

L

V

K

E

A

E

E

E

V

V

S

K

A

-

I

-

T

-

E

-

A

-

M

-

R

-

G

-

E

K

I

I

T

T

D

N

F

F

N

E

E

Q

S

S

L

L

R

R

R

K

R

R

V

V

A

S

L

L

K

K

G

D

L

L

D

P

A

I

S

E

V

K

L

V

D

E

R

K

V

A

Y

I

E

K

E

-

R

R

L

I

R

T

L

P

S

T

P

E

G

G

A

A

E

E

T

E

M

T

L

I

R

K

T

E

V

L

Q

K

A

N

L

R

G

G

M

Y

R

V

T

V

L

A

L

V

V

V

S

S

G

G

F

F

V

D

H

I

F

A

T

V

D

N

K

K

L

I

K

K

P

E

R

K

L

L

K

G

L

L

D

D

V

Y

T

A

R

F

A

A

N

N

T

R

L

L

E

I

I

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

Q

D

V

V

L

E

G

G

E

E

I

V

V

L

N

K

A

E

D

N

V

A

K

K

A

G

R

E

T

I

V

L

Q

E

E

K

V

I

C

A

A

K

Q

I

I

E

G

G

A

I

D

N

P

L

S

E

Q

D

A

T

I

V

V

A

M

V

G

G

D

D

G

G

S

A

N

N

D

D

L

I

K

S

M

M

M

F

A

K

V

K

A

A

G

G

L

L

S

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

V

I

V

L

R

K

A

E

Q

K

A

A

S

D

V

I

A
x
C

F

I

N
x
E

H

K

V

R

G

D

L

L

D

R

G

E

L

I

L

L

Q

K

binding zinc ion: C186 (≠ A269), E188 (≠ N271)

3m1yC Crystal structure of a phosphoserine phosphatase (serb) from helicobacter pylori
33% identity, 69% coverage: 76:271/284 of query aligns to 4:196/208 of 3m1yC

query
sites
3m1yC

K

K

L

L

V

A

A

V

M

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

T

N

I

A

E

E

C

T

I

I

D

E

E

S

I

L

A

A

D

R

F

A

C

W

G

G

L

V

K

F

A

D

E

E

V

V

S

K

A

T

I

I

T

T

E

L

A

K

A

A

M

M

R

N

G

G

E

-

I

-

T

T

D

D

F

F

N

H

E

K

S

S

L

L

R

I

R

L

R

R

V

V

A

S

L

K

L

L

K

K

G

N

L

M

D

P

A

L

S

K

V

L

L

A

D

K

R

E

V

V

Y

C

E

E

E

S

R

-

L

L

R

P

L

L

S

F

P

E

G

G

A

A

E

L

T

E

M

L

L

V

R

S

T

A

V

L

Q

K

A

E

L

K

G

N

M

Y

R

K

T

V

L

V

L

C

V

F

S

S

G
|
G

G

G

F

F

V

D

H

L

F

A

T

T

D

N

K

H

L

Y

K

R

P

D

R

L

L

L

K

H

L

L

D

D

V

A

T

A

R

F

A

S

N

N

T

T

L

L

E

I

I

V

V

E

D

N

G

D

K

A

L

L

T

N

G

G

Q

L

V

V

L

T

G

G

E

H

I

M

V

M

N

F

A

S

D

H

V

S

K
|
K

A

G

R

E

T

M

V

L

Q

L

E

V

V

L

C

Q

A

R

Q

L

I

L

G

N

A

I

D

S

P

K

S

T

Q

N

A

T

I

L

V

V

M

V

G

G

D
|
D

G

G

S

A

N

N

D
|
D

L

L

K

S

M

M

M

F

A

K

V

H

A

A

G

H

L

I

S

K

V

I

A

A

F

F

R

N

A

A

K

K

P

E

V

V

V

L

R

K

A

Q

Q

H

A

A

S

T

V

H

A

C

F

I

N

N

active site: D9 (= D81), F10 (≠ M82), D11 (= D83), G97 (= G172), K141 (= K216), D168 (= D243)
binding magnesium ion: D9 (= D81), D11 (= D83), D164 (= D239)

1l8oA Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase (see paper)
27% identity, 66% coverage: 78:265/284 of query aligns to 14:201/222 of 1l8oA

query
sites
1l8oA

V

V

A

C

M

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

T

R

I

E

E

E

C

G

I

I

D

D

E

E

I

L

A

A

D

K

F

I

C

C

G

G

L

V

K

E

A

D

E

A

V

V

S

S

A

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

T

P

D

-

F

F

N

K

E

A

S

A

L

L

R

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

S

Q

V

V

L

Q

D

R

R

L

V

I

Y

A

E

E

E

Q

R

P

L

P

R

H

L

L

S

T

P

P

G

G

A

I

E

R

T

E

M

L

L

V

R

S

T

R

V

L

Q

Q

A

E

L

R

G

N

M

V

R

Q

T

V

L

F

L

L

V

I

S
|
S

G
|
G

G

G

F

F

V

R

H

S

F

I

T

V

D

E

K

H

L

V

K

A

P

S

R

K

L

L

K

N

L

I

D

P

V

A

T

T

R

N

-

V

-

F

A

A

N

N

T

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

Q

E

V

Y

L

A

G

G

-

F

-

D

-

E

-

T

-

Q

E

P

I

T

V

A

N

E

A

S

D

G

V

G

K
|
K

A

G

R

K

T

V

V

I

Q

K

E

F

V

L

C

K

A

E

Q

K

I

F

G

H

A

F

D

-

P

-

S

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G
|
G

S
x
A

N
x
T

D
|
D

L

M

K

E

M

A

M

C

A

P

V

P

A

A

G

D

L

A

S

F

V

I

A

G

F

F

R

G

A

G

K

N

P

-

V

V

V

I

R
|
R

A

Q

Q

Q

active site: D17 (= D81), V18 (≠ M82), D19 (= D83), G107 (= G172), K155 (= K216), D180 (= D243)
binding phosphate ion: D17 (= D81), V18 (≠ M82), D19 (= D83), S106 (= S171), K155 (= K216)
binding serine: D176 (= D239), G177 (= G240), A178 (≠ S241), T179 (≠ N242), R199 (= R263)

1l8lA Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase (see paper)
27% identity, 66% coverage: 78:265/284 of query aligns to 14:201/222 of 1l8lA

query
sites
1l8lA

V

V

A

C

M

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

T

R

I

E

E
|
E

C

G

I

I

D

D

E

E

I

L

A

A

D

K

F

I

C

C

G

G

L

V

K

E

A

D

E

A

V

V

S

S

A

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

T

P

D

-

F

F

N

K

E

A

S

A

L

L

R

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

S

Q

V

V

L

Q

D

R

R

L

V

I

Y

A

E

E

E

Q

R

P

L

P

R

H

L

L

S

T

P

P

G

G

A

I

E

R

T

E

M

L

L

V

R

S

T

R

V

L

Q

Q

A

E

L

R

G

N

M

V

R

Q

T

V

L

F

L

L

V

I

S
|
S

G
|
G

G

G

F

F

V

R

H

S

F

I

T

V

D

E

K

H

L

V

K

A

P

S

R

K

L

L

K

N

L

I

D

P

V

A

T

T

R

N

-

V

-

F

A

A

N

N

T

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

Q

E

V

Y

L

A

G

G

-

F

-

D

-

E

-

T

-

Q

E

P

I

T

V

A

N

E

A

S

D

G

V

G

K
|
K

A

G

R

K

T

V

V

I

Q

K

E

F

V

L

C

K

A

E

Q

K

I

F

G

H

A

F

D

-

P

-

S

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G
|
G

S

A

N
x
T

D
|
D

L

M

K

E

M

A

M

C

A

P

V

P

A

A

G

D

L

A

S

F

V

I

A

G

F

F

R

G

A

G

K

N

P

-

V

V

V

I

R

R

A

Q

Q

Q

active site: D17 (= D81), V18 (≠ M82), D19 (= D83), G107 (= G172), K155 (= K216), D180 (= D243)
binding d-2-amino-3-phosphono-propionic acid: D17 (= D81), D19 (= D83), E26 (= E90), S106 (= S171), G107 (= G172), K155 (= K216), D176 (= D239), G177 (= G240), T179 (≠ N242), D180 (= D243)

6q6jB Human phosphoserine phosphatase with substrate analogue homo-cysteic acid (see paper)
27% identity, 66% coverage: 78:265/284 of query aligns to 13:200/217 of 6q6jB

query
sites
6q6jB

V

V

A

C

M

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

T

R

I

E

E

E

C

G

I

I

D

D

E

E

I

L

A

A

D

K

F

I

C

C

G

G

L

V

K

E

A

D

E

A

V

V

S

S

A

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

T

P

D

-

F
|
F

N

K

E

A

S

A

L

L

R

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

S

Q

V

V

L

Q

D

R

R

L

V

I

Y

A

E

E

E

Q

R

P

L

P

R

H

L

L

S

T

P

P

G

G

A

I

E

R

T

E

M

L

L

V

R

S

T

R

V

L

Q

Q

A

E

L

R

G

N

M

V

R

Q

T

V

L

F

L

L

V

I

S
|
S

G
|
G

F

F

V

R

H

S

F

I

T

V

D

E

K

H

L

V

K

A

P

S

R

K

L

L

K

N

L

I

D

P

V

A

T

T

R

N

-

V

-

F

A

A

N

N

T

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

Q

E

V

Y

L

A

G

G

-

F

-

D

-

E

-

T

-

Q

E

P

I

T

V

A

N

E

A

S

D

G

V

G

K
|
K

A

G

R

K

T

V

V

I

Q

K

E

L

V

L

C

K

A

E

Q

K

I

F

G

H

A

F

D

-

P

-

S

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G

G

S

A

N
x
T

D

D

L

M

K

E

M

A

M

C

A

P

V

P

A

A

G

D

L

A

S

F

V

I

A

G

F

F

R

G

A

G

K

N

P

-

V

V

V

I

R

R

A

Q

Q

Q

binding calcium ion: D16 (= D81), D18 (= D83), D175 (= D239)
binding (2~{S})-2-azanyl-4-sulfo-butanoic acid: D16 (= D81), V17 (≠ M82), D18 (= D83), F54 (= F120), S105 (= S171), G106 (= G172), G107 (= G173), K154 (= K216), T178 (≠ N242)

6hyyA Human phosphoserine phosphatase with serine and phosphate (see paper)
27% identity, 66% coverage: 78:265/284 of query aligns to 13:200/221 of 6hyyA

query
sites
6hyyA

V

V

A

C

M

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

T

R

I

E

E

E

C

G

I

I

D

D

E

E

I

L

A

A

D

K

F

I

C

C

G

G

L

V

K

E

A

D

E

A

V

V

S

S

A

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

T

P

D

-

F
|
F

N

K

E

A

S

A

L

L

R

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

S

Q

V

V

L

Q

D

R

R

L

V

I

Y

A

E

E

E

Q

R

P

L

P

R

H

L

L

S

T

P

P

G

G

A

I

E

R

T

E

M

L

L

V

R

S

T

R

V

L

Q

Q

A

E

L

R

G

N

M

V

R

Q

T

V

L

F

L

L

V

I

S
|
S

G
|
G

F

F

V

R

H

S

F

I

T

V

D

E

K

H

L

V

K

A

P

S

R

K

L

L

K

N

L

I

D

P

V

A

T

T

R

N

-

V

-

F

A

A

N

N

T

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

Q

E

V

Y

L

A

G

G

-

F

-

D

-

E

-

T

-

Q

E

P

I

T

V

A

N

E

A

S

D

G

V

G

K
|
K

A

G

R

K

T

V

V

I

Q

K

E

L

V

L

C

K

A

E

Q

K

I

F

G

H

A

F

D

-

P

-

S

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G

G

S

A

N

T

D

D

L

M

K

E

M

A

M

C

A

P

V

P

A

A

G

D

L

A

S

F

V

I

A

G

F

F

R

G

A

G

K

N

P

-

V

V

V

I

R

R

A

Q

Q

Q

binding magnesium ion: D16 (= D81), D18 (= D83), D175 (= D239)
binding serine: D16 (= D81), V17 (≠ M82), D18 (= D83), F54 (= F120), S105 (= S171), G106 (= G172), G107 (= G173), K154 (= K216)

6hyjB Psph human phosphoserine phosphatase (see paper)
27% identity, 66% coverage: 78:265/284 of query aligns to 17:204/223 of 6hyjB

query
sites
6hyjB

V

V

A

C

M

F

D
|
D

M

V

D
|
D

S

S

T

T

L

V

I

I

T

R

I

E

E
|
E

C

G

I

I

D

D

E

E

I

L

A

A

D

K

F

I

C

C

G

G

L

V

K

E

A

D

E

A

V

V

S

S

A

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

T

P

D

-

F

F

N

K

E

A

S

A

L

L

R

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

S

Q

V

V

L

Q

D

R

R

L

V

I

Y

A

E

E

E

Q

R

P

L

P

R

H

L

L

S

T

P

P

G

G

A

I

E

R

T

E

M

L

L

V

R

S

T

R

V

L

Q

Q

A

E

L

R

G

N

M

V

R

Q

T

V

L

F

L

L

V

I

S

S

G

G

F

F

V

R

H

S

F

I

T

V

D

E

K

H

L

V

K

A

P

S

R

K

L

L

K

N

L

I

D

P

V

A

T

T

R

N

-

V

-
x
F

A

A

N

N

T

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

Q

E

V

Y

L

A

G

G

-

F

-

D

-

E

-

T

-

Q

E
x
P

I

T

V

A

N

E

A

S

D

G

V

G

K

K

A

G

R

K

T

V

V

I

Q

K

E
x
L

V

L

C

K

A

E

Q
x
K

I

F

G

H

A

F

D

-

P

-

S

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G

G

S

A

N

T

D

D

L

M

K

E

M

A

M

C

A

P

V

P

A

A

G

D

L

A

S

F

V

I

A

G

F

F

R

G

A

G

K

N

P

-

V

V

V

I

R
|
R

A

Q

Q

Q

binding calcium ion: D20 (= D81), D22 (= D83), D179 (= D239)
binding phosphoserine: F131 (vs. gap), P151 (≠ E209), L164 (≠ E222), K168 (≠ Q226)
binding serine: E29 (= E90), R202 (= R263)

P78330 Phosphoserine phosphatase; PSP; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Homo sapiens (Human) (see 4 papers)
27% identity, 66% coverage: 78:265/284 of query aligns to 17:204/225 of P78330

query
sites
P78330

V

V

A

C

M

F

D
|
D

M
x
V

D
|
D

S
|
S

T

T

L

V

I

I

T

R

I

E

E
|
E

C

G

I

I

D
|
D

E

E

I

L

A
|
A

D

K

F

I

C

C

G

G

L

V

K

E

A

D

E

A

V

V

S

S

A

E

I

M

T

T

E

R

A

R

A

A

M
|
M

R
x
G

G

G

E

A

I

V

T

P

D

-

F

F

N

K

E

A

S

A

L

L

R

T

R

E

R
|
R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

S

Q

V

V

L

Q

D

R

R

L

V

I

Y

A

E

E

E

Q

R

P

L

P

R

H

L

L

S

T

P

P

G

G

A

I

E

R

T

E

M

L

L

V

R

S

T

R

V

L

Q

Q

A

E

L

R

G

N

M

V

R

Q

T

V

L

F

L

L

V

I

S
|
S

G
|
G

F

F

V

R

H

S

F

I

T

V

D

E

K

H

L

V

K

A

P

S

R

K

L

L

K

N

L

I

D

P

V

A

T

T

R

N

-

V

-

F

A

A

N
|
N

T

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

Q

E

V

Y

L

A

G

G

-

F

-

D

-

E

-

T

-

Q

E

P

I

T

V

A

N

E

A

S

D

G

V

G

K
|
K

A

G

R

K

T

V

V

I

Q

K

E

L

V

L

C

K

A

E

Q

K

I

F

G

H

A

F

D

-

P

-

S

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G

G

S

A

N
x
T

D

D

L

M

K

E

M

A

M

C

A

P

V

P

A

A

G

D

L

A

S

F

V

I

A

G

F

F

R

G

A

G

K

N

P

-

V

V

V

I

R
|
R

A

Q

Q

Q

D20 (= D81) binding
DVD 20:22 (≠ DMD 81:83) binding
D22 (= D83) binding
S23 (= S84) mutation to A: Reduces L-phosphoserine phosphatase activity by about 50%.; mutation to T: Reduces L-phosphoserine phosphatase activity by about 80%.
E29 (= E90) mutation to D: Reduces L-phosphoserine phosphatase activity by about 95%.; mutation to Q: Loss of L-phosphoserine phosphatase activity.
D32 (= D93) to N: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894035
A35 (= A96) to T: in PSPHD; decreased L-phosphoserine phosphatase activity
M52 (= M113) binding ; to T: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894036
G53 (≠ R114) binding
R65 (= R127) mutation R->A,K: Loss of L-phosphoserine phosphatase activity.
SGG 109:111 (= SGG 171:173) binding ; binding
N133 (= N193) mutation to A: Reduces L-phosphoserine phosphatase activity by about 75%.
K158 (= K216) binding ; binding
D179 (= D239) binding
T182 (≠ N242) binding ; binding ; mutation to S: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to V: Reduces L-phosphoserine phosphatase activity by about 25%.
R202 (= R263) mutation to A: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to K: Reduces L-phosphoserine phosphatase activity by about 95%.

6iuyA Structure of dsgpdh of dunaliella salina (see paper)
29% identity, 60% coverage: 78:247/284 of query aligns to 17:183/585 of 6iuyA

query
sites
6iuyA

V

V

A

C

M

F

D
|
D

M

V

D
|
D

S
x
R

T

T

L

V

I

T

T

T

I

D

E

A

C

S

I

V

D

G

E

L

I

L

A

A

D

K

F

F

C

M

G

G

L

I

K

E

A

D

E

E

V

A

S

Q

A

S

I

L

T

T

E

E

A

Q

A

A

M

N

R

R

G

G

E

E

I

I

T

-

D

N

F

L

N

T

E

K

S

A

L

F

R

E

R

D

R

R

V

L

A

A

L

K

L

L

K

N

G

-

L

F

D

T

A

P

S

T

V

D

L

I

D

D

R

R

V

F

Y

L

E

E

-

H

-

P

-

A

R

H

L

T

R

R

L

L

S

V

P

P

G

G

A

V

E

E

T

N

M

L

L

I

R

A

T

A

V

L

Q

K

A

A

L

R

G

G

M

V

R

E

T

V

L

F

L

L

V

I

S

S

G
|
G

G

G

F

F

V

R

H

E

F

M

T

A

D

L

K

P

L

I

K

A

P

S

R

H

L

L

K

K

L

I

D

P

V

A

T

K

R

-

A

-

N

-

T

-

L

-

E

N

I

V

V

F

D

C

G

N

K

T

L

M

T

S

G

W

Q

Q

V

L

-

D

L

H

G

G

E

E

I

P

V

V

N

R

-

L

A

S

D

H

V

F

K
|
K

A

S

R

R

T

A

V

I

Q

E

E

R

V

I

C

R

A

R

Q

K

I

Y

G

-

A

-

D

-

P

P

S

Y

Q

N

A

N

I

I

V

I

M

M

-

V

G

G

D
|
D

G

G

S

F

N
x
S

D

D

L

L

K

E

M

A

M

M

binding glycerol: D22 (= D83), R23 (≠ S84), G112 (= G172), K154 (= K216), S178 (≠ N242)
binding magnesium ion: D20 (= D81), D22 (= D83), D175 (= D239)

Sites not aligning to the query:

binding glycerol: 202
binding nicotinamide-adenine-dinucleotide: 233, 234, 235, 236, 237, 268, 270, 290, 319, 320, 321, 324, 347, 348, 349, 380, 381, 382, 497, 528, 529, 530, 532

4ap9A Crystal structure of phosphoserine phosphatase from t.Onnurineus in complex with ndsb-201 (see paper)
29% identity, 63% coverage: 76:255/284 of query aligns to 10:174/200 of 4ap9A

query
sites
4ap9A

K

K

L

V

V

A

A

V

M

I

D
|
D

M
x
I

D
x
E

S

G

T

T

L

L

I

T

T

D

I

F

E

E

C

F

I

W

D

R

E

E

I

M

A

A

D
x
R

F
x
I

C
x
T

G
|
G

L

-

K

K

A

R

E

E

V

I

S

E

A

E

I

L

T

L

E

E

A

K

A
x
G

M
x
L

R

S

G

G

E

E

I

V

T

-

D

E

F
x
W

N

L

E

D

S

S

L

L

R

L

R

K

R

R

V

V

A

G

L

L

L

I

K

R

G

G

L

I

D

D

A

E

S

G

V

T

L

F

D

L

R

R

V

T

Y

-

E

R

E

E

R

K

L

V

R

N

L

V

S

S

P

P

G

E

A

A

E

R

T

E

M

L

L

V

R

E

T

T

V

L

Q

R

A

E

L

K

G

G

M

F

R

K

T

V

L

V

L

L

V

I

S

S

G
|
G

G

-

F

-

V

-

H

S

F

F

T

E

D

E

K

V

L

L

K

E

P

P

R

F

L

K

K

E

L

L

-

G

D

D

V

E

T

F

R

M

A

A

N

N

T

R

L

A

E

I

I

F

V

E

D

D

G

G

K

K

L

F

T

Q

G

G

Q

I

V

R

L

L

G

-

E

-

I

-

V

-

N

-

A

-

D

-

V

-

K

R

A

F

R

R

T
x
D