SitesBLAST

5eqhA Human glut1 in complex with inhibitor (2~{s})-3-(2-bromophenyl)-2-[2- (4-methoxyphenyl)ethanoylamino]-~{n}-[(1~{s})-1- phenylethyl]propanamide (see paper)
24% identity, 43% coverage: 54:238/433 of query aligns to 59:251/447 of 5eqhA

query
sites
5eqhA

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binding (2~{S})-3-(2-bromophenyl)-2-[2-(4-methoxyphenyl)ethanoylamino]-~{N}-[(1~{S})-1-phenylethyl]propanamide: S72 (≠ A70), T129 (≠ L132)

Sites not aligning to the query:

binding (2~{S})-3-(2-bromophenyl)-2-[2-(4-methoxyphenyl)ethanoylamino]-~{N}-[(1~{S})-1-phenylethyl]propanamide: 274, 371, 380, 400, 403, 404

5eqgA Human glut1 in complex with inhibitor (2~{s})-3-(4-fluorophenyl)-2-[2- (3-hydroxyphenyl)ethanoylamino]-~{n}-[(1~{s})-1- phenylethyl]propanamide (see paper)
24% identity, 43% coverage: 54:238/433 of query aligns to 59:251/447 of 5eqgA

query
sites
5eqgA

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binding (2~{S})-3-(4-fluorophenyl)-2-[2-(3-hydroxyphenyl)ethanoylamino]-~{N}-[(1~{S})-1-phenylethyl]propanamide: T129 (≠ L132)

Sites not aligning to the query:

binding (2~{S})-3-(4-fluorophenyl)-2-[2-(3-hydroxyphenyl)ethanoylamino]-~{N}-[(1~{S})-1-phenylethyl]propanamide: 274, 380, 403, 404

P11166 Solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter from Homo sapiens (Human) (see 23 papers)
24% identity, 43% coverage: 54:238/433 of query aligns to 67:259/492 of P11166

query
sites
P11166

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M77 (≠ L64) to T: in EIG12; decreased glucose transport; dbSNP:rs1187210267
G91 (= G81) to D: in GLUT1DS1; significantly decreases the transport of 3-O-methyl-D-glucose; dbSNP:rs80359814
R126 (= R121) to C: in GLUT1DS1, GLUT1DS2 and DYT9; reduced transporter activity; dbSNP:rs80359818; to H: in GLUT1DS1; significantly decreases the transport of 3-O-methyl-D-glucose and dehydroascorbic acid; 57% of wild-type glucose uptake activity; dbSNP:rs80359816
G130 (= G125) to S: in GLUT1DS1; 75% of wild-type glucose uptake activity; dbSNP:rs80359819
T137 (≠ L132) binding
P149 (≠ K144) to A: in EIG12; uncertain significance
R153 (≠ K148) to C: in GLUT1DS1; 44% of wild-type glucose uptake activity
L169 (≠ I164) natural variant: Missing (in GLUT1DS1; 48% of wild-type glucose uptake activity; dbSNP:rs80359832)
I192 (vs. gap) mutation to C: Strongly decreases glucose transport.
L204 (≠ V198) mutation to C: Abolishes glucose transport.
P205 (= P199) mutation to C: Abolishes glucose transport.
R212 (vs. gap) to C: in GLUT1DS1 and DYT9; dbSNP:rs387907312
R218 (= R206) to S: in EIG12; decreased glucose transport
R223 (≠ T211) to P: in EIG12; mild phenotype; reduced transporter activity; impaired phosphorylation by PKC; dbSNP:rs397514564; to Q: in EIG12; uncertain significance; no effect on glucose transport; impaired phosphorylation by PKC; dbSNP:rs397514564; to W: in GLUT1DS1; impaired phosphorylation by PKC; dbSNP:rs796053248
S226 (≠ F214) modified: Phosphoserine; by PKC/PRKCB; mutation to A: Abolishes phosphorylation by PKA, leading to impaired response to TPA.
R232 (= R220) to C: in EIG12; the mutant protein is expressed at the cell surface but has mildly decreased glucose uptake (70%) compared to wild-type; dbSNP:rs387907313
E243 (≠ S222) to V: in EIG12; decreased glucose transport

Sites not aligning to the query:

34 N → S: in GLUT1DS1; 55% of wild-type glucose uptake activity; dbSNP:rs80359812
45 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→T: Loss of glycosylation site.
51 R → H: in EIG12; uncertain significance; dbSNP:rs201815571
60 T → M: in EIG12; uncertain significance; decreased glucose transport; dbSNP:rs142986731
275 A → T: in GLUT1DS2; the mutation decreases glucose transport but does not affect cation permeability; dbSNP:rs121909740
282 binding
282:285 natural variant: Missing (in GLUT1DS2; accompanied by hemolytic anemia and altered erythrocyte ion concentrations; the mutation decreases glucose transport and causes a cation leak that alteres intracellular concentrations of sodium potassium and calcium)
286 G → D: in SDCHCN; no effect on protein abundance; no effect on localization to the plasma membrane; loss of D-glucose transporter activity; increased cation leakage; dbSNP:rs864309514
295 T → M: in GLUT1DS1; 75% of wild-type glucose uptake activity; dbSNP:rs80359823
303 V → L: found in a patient with GLUT1 deficiency syndrome; dbSNP:rs1205631854
314 G → S: in GLUT1DS2; the mutation decreases glucose transport but does not affect cation permeability; dbSNP:rs121909739
324 S → L: in GLUT1DS2; mild phenotype; reduced transporter activity; dbSNP:rs796053253
329 E → Q: in GLUT1DS1; stabilizes the inward-open conformation
333 R → Q: in GLUT1DS1 and GLUT1DS2; dbSNP:rs1553155986; R → W: in GLUT1DS1; 43% of wild-type glucose uptake activity; dbSNP:rs80359825
340 G→C: Strongly decreases glucose transport.
388 binding
411 Not glycosylated; binding ; N → S: in EIG12; decreased glucose transport; dbSNP:rs398123069
435 natural variant: Missing (in SDCHCN; no effect on protein abundance; no effect on localization to the plasma membrane; loss of D-glucose transporter activity; increased cation leakage)
458 R → W: in EIG12; decreased glucose transport; dbSNP:rs13306758
485 P → L: in GLUT1DS1; creates a dileucine internalization motif that promotes recruitment of clathrin and mislocalization of the protein to endocytic compartments

P17809 Solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GT1 from Mus musculus (Mouse) (see 3 papers)
24% identity, 41% coverage: 54:232/433 of query aligns to 67:243/492 of P17809

query
sites
P17809

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Sites not aligning to the query:

45 modified: carbohydrate, N-linked (GlcNAc...) asparagine
485 P→L: Lethality immediately after birth in knockin mice; caused by creation of a dileucine internalization motif that promotes mislocalization of the protein.

P32037 Solute carrier family 2, facilitated glucose transporter member 3; Glucose transporter type 3, brain; GLUT-3 from Mus musculus (Mouse) (see paper)
24% identity, 41% coverage: 54:232/433 of query aligns to 65:241/493 of P32037

query
sites
P32037

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Sites not aligning to the query:

43 modified: carbohydrate, N-linked (GlcNAc...) asparagine

P0AGF4 D-xylose-proton symporter; D-xylose transporter from Escherichia coli (strain K12) (see paper)
24% identity, 85% coverage: 1:369/433 of query aligns to 1:411/491 of P0AGF4

query
sites
P0AGF4

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R

V

V

A

V

T

S

L

G

G

N

G

F

L

L

L

E
x
F

M

G

Y

Y

D

D

F

T

M

A

V

V

Y

I

G

S

F

G

Y

T

A

V

A

E

A

S

I

L

A

N

K

T

T

V

F

F

F

V

P

A

S

P

G

Q

N

N

E

L

F

S

A

E

S

S

L

A

M

A

L

N

S

S

L

L

A

L

T

G

F

F

G

C

A

V

G

A

F

S

L

A

M

L

R

-

P

-

L

I

G

G

A

C

I

I

-

G

-

A

L

L

G

G

A

G

Y

Y

I

C

D

S

H

N

H

R

-

F

G
|
G

R

R

K

D

G

S

L

L

I

K

M

I

T

A

L

A

V

V

L

L

M

F

A

F

L

I

G

S

T

G

L

V

L

G

I

S

A

A

C

W

V

P

P

E

-

L

G

G

Y

F

A

T

S

S

I

I

G

N

V

P

A

D

A

N

P

T

L

V

L

P

V

V

-

Y

L

L

A

A

G

G

-

Y

-

V

-

P

-

E

-

F

-

V

-

I

-

Y

R
|
R

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

V

L

E

A

L

S

G

M

G

L

V

S

S

P

V

M

Y

Y

L

I

A

A

E
|
E

I

L

A

A

K

P

P

A

G

H

K

I

K
x
R

G

G

F

K

Y

L

V

V

A

S

W

F

Q

N

S

-

A

-

S

-

Q

-

Q
|
Q

V

F

A

A

V

I

I

I

F

F

A

G

G

Q

L

L

G

V

V

Y

V

C

L

V

H

N

S

Y

M

F

L

I

S

A

P

R

E

S

E

G

M

D

G

A

E

S

W

W

-

L

-

N

-

T

-

D

G

G

W

W

R

R

I

Y

P

M

F

F

L

A

I

S

G

E

C

C

L

-

I

-

V

I

P

P

F

A

L

L

-

L

-

F

-

L

-

M

-

L

-

Y

-

T

-

V

-

P

-

E

-

S

-

P

-

R

F

W

L

L

I

M

R

S

R

R

S

G

L

K

E

Q

E

E

T

Q

E

A

E

E

F

G

K

I

T

L

R

R

K

K

-

I

-

M

-

G

-

N

-

T

-

L

H

A

R

T

P

Q

S

A

I

V

S

Q

E

E

I

I

Y

K

R

H

S

S

M

L

M

D

E

H

N

G

W

R

R

K

-

T

-

G

-

R

-

L

-

M

-

F

-

G

-

V

-

G

I

V

I

I

V

V

A

I

G

G

C

V

M

M

M

L

V

S

V

I

M

F

T
x
Q

-

F

V

V

S

G

F

I

Y
x
N

M

V

I

V

T

L

A
x
Y

Y

Y

T

A

P

P

T

E

F

V

G

F

K

K

T

T

V

L

L

G

K

A

L

S

D

T

D

D

V

I

D

A

N

-

L

L

I

L

V

Q

T

T

M

I

C

I

V

V

G

G

L

V

S

I

N
|
N

F

L

I

T

W

F

L

T

P

V

L

L

M

A

G

I

A

M

L

T

S

V

D

D

R

K

V

F

G
|
G

R
|
R

K

K

P

P

L

L

L

Q

V

I

I

I

F

G

T

A

I

L

L

G

T

M

L

A

L

I

T

G

A

M

Y

F

P

S

-

L

A

G

V

T

S

A

W

F

L

Y

V

T

A

Q

E

A

P

P

S

G

F

I

G

V

R

A

L

L

M

S

V

M

E

L

L

F

W

Y

L

V

S

A

F

A

L

F

Y

A

G

M

S

S

Y

W

N

G

G

P

A

V

M

C

V
x
W

V

V

T

L

L

L

T

S

E
|
E

V

I

M

F

P

P

P

N

A

A

V

I

R
|
R

T

G

A

K

G

A

F

L

S

A

L

I

A

A

F24 (≠ E24) mutation to A: Decreases xylose transport.
G83 (= G81) mutation to A: Abolishes xylose transport.
R133 (= R121) mutation R->C,H,L: Abolishes xylose transport.
E153 (= E141) mutation to A: Abolishes xylose transport.
R160 (≠ K148) mutation to A: Abolishes xylose transport.
Q168 (= Q160) binding ; mutation to A: Abolishes xylose transport.
Q288 (≠ T247) mutation to A: Abolishes xylose transport.
QQ 288:289 (≠ TT 247:248) binding
Q289 (≠ T248) mutation to A: Strongly decreases xylose transport.
N294 (≠ Y252) binding ; mutation to A: Abolishes xylose transport.
Y298 (≠ A256) mutation to A: Abolishes xylose transport.
N325 (= N284) mutation to A: No effect on xylose transport.
G340 (= G299) mutation to A: Abolishes xylose transport.
R341 (= R300) mutation R->A,W: Abolishes xylose transport.
W392 (≠ V350) binding ; mutation to A: Abolishes xylose transport.
E397 (= E355) mutation to A: Abolishes xylose transport.
R404 (= R362) mutation to A: Strongly decreases xylose transport.

Sites not aligning to the query:

415 binding
416 W→A: Strongly decreases xylose transport.

4gc0A The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to 6-bromo-6-deoxy-d-glucose (see paper)
24% identity, 70% coverage: 68:369/433 of query aligns to 62:407/475 of 4gc0A

query
sites
4gc0A

L

I

G

G

A

C

I

I

-

G

-

A

L

L

G

G

A

G

Y

Y

I

C

D

S

H

N

H

R

-

F

G

G

R

R

K

D

G

S

L

L

I

K

M

I

T

A

L

A

V

V

L

L

M

F

A

F

L

I

G

S

T

G

L

V

L

G

I

S

A

A

C

W

V

P

P

E

-

L

G

G

Y

F

A

T

S

S

I

I

G

N

V

P

A

D

A

N

P

T

L

V

L

P

V

V

-

Y

L

L

A

A

G

G

-

Y

-

V

-

P

-

E

-

F

-

V

-

I

-

Y

R

R

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

V

L

E

A

L

S

G

M

G

L

V

S

S

P

V

M

Y

Y

L

I

A

A

E

E

I

L

A

A

K

P

P

A

G

H

K

I

K

R

G

G

F

K

Y

L

V

V

A

S

W

F

Q

N

S

-

A

-

S

-

Q

-

Q
|
Q

V

F

A

A

V

I

I

I

F

F

A

G

G

Q

L

L

G

V

V

Y

V

C

L

V

H

N

S

Y

M

F

L

I

S

A

P

R

E

S

E

G

M

D

G

A

E

S

W

W

-

L

-

N

-

T

-

D

G

G

W

W

R

R

I

Y

P

M

F

F

L

A

I

S

G

E

C

C

L

-

I

-

V

I

P

P

F

A

L

L

-

L

-

F

-

L

-

M

-

L

-

Y

-

T

-

V

-

P

-

E

-

S

-

P

-

R

F

W

L

L

I

M

R

S

R

R

S

G

L

K

E

Q

E

E

T

Q

E

A

E

E

F

G

K

I

T

L

R

R

K

K

-

I

-

M

-

G

-

N

-

T

-

L

H

A

R

T

P

Q

S

A

I

V

S

Q

E

E

I

I

Y

K

R

H

S

S

M

L

M

D

E

H

N

G

W

R

R

K

-

T

-

G

-

R

-

L

-

M

-

F

-

G

-

V

-

G

I

V

I

I

V

V

A

I

G

G

C

V

M

M

M

L

V

S

V

I

M

F

T
x
Q

T

Q

-

F

V

V

S

G

F

I

Y
x
N

M

V

I

V

T
x
L

A

Y

Y

Y

T

A

P

P

T

E

F

V

G

F

K

K

T

T

V

L

L

G

K

A

L

S

D

T

D

D

V

I

D

A

N

-

L

L

I

L

V

Q

T

T

M

I

C

I

V

V

G

G

L

V

S

I

N

N

F

L

I

T

W

F

L

T

P

V

L

L

M

A

G

I

A

M

L

T

S

V

D

D

R

K

V

F

G

G

R

R

K

K

P

P

L

L

L

Q

V

I

I

I

F

G

T

A

I

L

L

G

T

M

L

A

L

I

T

G

A

M

Y

F

P

S

-

L

A

G

V

T

S

A

W

F

L

Y

V

T

A

Q

E

A

P

P

S

G

F

I

G

V

R

A

L

L

M

S

V

M

E

L

L

F

W

Y

L

V

S

A

F

A

L

F

Y

A

G

M

S

S

Y

W

N

G

G

P

A

V

M

C

V
x
W

V

V

T

L

L

L

T

S

E

E

V

I

M

F

P

P

P

N

A

A

V

I

R

R

T

G

A

K

G

A

F

L

S

A

L

I

A

A

binding 6-bromo-6-deoxy-beta-D-glucopyranose: Q164 (= Q160), Q284 (≠ T247), N290 (≠ Y252), L293 (≠ T255), W388 (≠ V350)

4gbzA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-glucose (see paper)
24% identity, 70% coverage: 68:369/433 of query aligns to 62:407/475 of 4gbzA

query
sites
4gbzA

L

I

G

G

A

C

I

I

-

G

-

A

L

L

G

G

A

G

Y

Y

I

C

D

S

H

N

H

R

-

F

G

G

R

R

K

D

G

S

L

L

I

K

M

I

T

A

L

A

V

V

L

L

M

F

A

F

L

I

G

S

T

G

L

V

L

G

I

S

A

A

C

W

V

P

P

E

-

L

G

G

Y

F

A

T

S

S

I

I

G

N

V

P

A

D

A

N

P

T

L

V

L

P

V

V

-

Y

L

L

A

A

G

G

-

Y

-

V

-

P

-

E

-

F

-

V

-

I

-

Y

R

R

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

V

L

E

A

L

S

G

M

G

L

V

S

S

P

V

M

Y

Y

L

I

A

A

E

E

I

L

A

A

K

P

P

A

G

H

K

I

K

R

G

G

F

K

Y

L

V

V

A

S

W

F

Q

N

S

-

A

-

S

-

Q

-

Q
|
Q

V

F

A

A

V

I

I

I

F

F

A

G

G

Q

L

L

G

V

V

Y

V

C

L

V

H

N

S

Y

M

F

L

I

S

A

P

R

E

S

E

G

M

D

G

A

E

S

W

W

-

L

-

N

-

T

-

D

G

G

W

W

R

R

I

Y

P

M

F

F

L

A

I

S

G

E

C

C

L

-

I

-

V

I

P

P

F

A

L

L

-

L

-

F

-

L

-

M

-

L

-

Y

-

T

-

V

-

P

-

E

-

S

-

P

-

R

F

W

L

L

I

M

R

S

R

R

S

G

L

K

E

Q

E

E

T

Q

E

A

E

E

F

G

K

I

T

L

R

R

K

K

-

I

-

M

-

G

-

N

-

T

-

L

H

A

R

T

P

Q

S

A

I

V

S

Q

E

E

I

I

Y

K

R

H

S

S

M

L

M

D

E

H

N

G

W

R

R

K

-

T

-

G

-

R

-

L

-

M

-

F

-

G

-

V

-

G

I

V

I

I

V

V

A

I

G

G

C

V

M

M

M

L

V

S

V

I

M

F

T
x
Q

T

Q

-

F

V

V

S

G

F

I

Y
x
N

M

V

I

V

T

L

A

Y

Y

Y

T

A

P

P

T

E

F

V

G

F

K

K

T

T

V

L

L

G

K

A

L

S

D

T

D

D

V

I

D

A

N

-

L

L

I

L

V

Q

T

T

M

I

C

I

V

V

G

G

L

V

S

I

N

N

F

L

I

T

W

F

L

T

P

V

L

L

M

A

G

I

A

M

L

T

S

V

D

D

R

K

V

F

G

G

R

R

K

K

P

P

L

L

L

Q

V

I

I

I

F

G

T

A

I

L

L

G

T

M

L

A

L

I

T

G

A

M

Y

F

P

S

-

L

A

G

V

T

S

A

W

F

L

Y

V

T

A

Q

E

A

P

P

S

G

F

I

G

V

R

A

L

L

M

S

V

M

E

L

L

F

W

Y

L

V

S

A

F

A

L

F

Y

A

G

M

S

S

Y

W

N

G

G

P

A

V

M

C

V
x
W

V

V

T

L

L

L

T

S

E

E

V

I

M

F

P

P

P

N

A

A

V

I

R

R

T

G

A

K

G

A

F

L

S

A

L

I

A

A

binding beta-D-glucopyranose: Q164 (= Q160), Q284 (≠ T247), N290 (≠ Y252), W388 (≠ V350)

4gbyA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-xylose (see paper)
24% identity, 70% coverage: 68:369/433 of query aligns to 62:407/475 of 4gbyA

query
sites
4gbyA

L

I

G

G

A

C

I

I

-

G

-

A

L

L

G

G

A

G

Y

Y

I

C

D

S

H

N

H

R

-

F

G

G

R

R

K

D

G

S

L

L

I

K

M

I

T

A

L

A

V

V

L

L

M

F

A

F

L

I

G

S

T

G

L

V

L

G

I

S

A

A

C

W

V

P

P

E

-

L

G

G

Y

F

A

T

S

S

I

I

G

N

V

P

A

D

A

N

P

T

L

V

L

P

V

V

-

Y

L

L

A

A

G

G

-

Y

-

V

-

P

-

E

-

F

-

V

-

I

-

Y

R

R

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

V

L

E

A

L

S

G

M

G

L

V

S

S

P

V

M

Y

Y

L

I

A

A

E

E

I

L

A

A

K

P

P

A

G

H

K

I

K

R

G

G

F

K

Y

L

V

V

A

S

W

F

Q

N

S

-

A

-

S

-

Q

-

Q
|
Q

V

F

A

A

V

I

I

I

F

F

A

G

G

Q

L

L

G

V

V

Y

V

C

L

V

H

N

S

Y

M

F

L

I

S

A

P

R

E

S

E

G

M

D

G

A

E

S

W

W

-

L

-

N

-

T

-

D

G

G

W

W

R

R

I

Y

P

M

F

F

L

A

I

S

G

E

C

C

L

-

I

-

V

I

P

P

F

A

L

L

-

L

-

F

-

L

-

M

-

L

-

Y

-

T

-

V

-

P

-

E

-

S

-

P

-

R

F

W

L

L

I

M

R

S

R

R

S

G

L

K

E

Q

E

E

T

Q

E

A

E

E

F

G

K

I

T

L

R

R

K

K

-

I

-

M

-

G

-

N

-

T

-

L

H

A

R

T

P

Q

S

A

I

V

S

Q

E

E

I

I

Y

K

R

H

S

S

M

L

M

D

E

H

N

G

W

R

R

K

-

T

-

G

-

R

-

L

-

M

-

F

-

G

-

V

-

G

I

V

I

I

V

V

A

I

G

G

C

V

M

M

M

L

V

S

V

I

M

F

T
x
Q

T

Q

-

F

V

V

S

G

F

I

Y
x
N

M

V

I

V

T

L

A

Y

Y

Y

T

A

P

P

T

E

F

V

G

F

K

K

T

T

V

L

L

G

K

A

L

S

D

T

D

D

V

I

D

A

N

-

L

L

I

L

V

Q

T

T

M

I

C

I

V

V

G

G

L

V

S

I

N

N

F

L

I

T

W

F

L

T

P

V

L

L

M

A

G

I

A

M

L

T

S

V

D

D

R

K

V

F

G

G

R

R

K

K

P

P

L

L

L

Q

V

I

I

I

F

G

T

A

I

L

L

G

T

M

L

A

L

I

T

G

A

M

Y

F

P

S

-

L

A

G

V

T

S

A

W

F

L

Y

V

T

A

Q

E

A

P

P

S

G

F

I

G

V

R

A

L

L

M

S

V

M

E

L

L

F

W

Y

L

V

S

A

F

A

L

F

Y

A

G

M

S

S

Y

W

N

G

G

P

A

V

M

C

V
x
W

V

V

T

L

L

L

T

S

E

E

V

I

M

F

P

P

P

N

A

A

V

I

R

R

T

G

A

K

G

A

F

L

S

A

L

I

A

A

binding beta-D-xylopyranose: Q164 (= Q160), Q284 (≠ T247), N290 (≠ Y252), W388 (≠ V350)

P43427 Solute carrier family 2, facilitated glucose transporter member 5; Fructose transporter; Glucose transporter type 5, small intestine; GLUT-5 from Rattus norvegicus (Rat) (see paper)
30% identity, 34% coverage: 51:199/433 of query aligns to 71:201/502 of P43427

query
sites
P43427

S

S

L

L

M

T

L

V

S

S

L

M

A

F

T

P

F

F

G

G

A

-

G

G

F

F

L

I

M

-

R

-

P

-

L

-

G

G

A

S

I

L

I

M

L

V

G

G

A

F

Y

L

I

V

D

N

H

N

H

L

G

G

R

R

R

K

K

G

G

A

L

L

I

L

M

F

T

N

L

N

V

I

L

F

M

S

A

I

L

L

G

P

T

A

L

I

L

L

I

M

A

G

C

C

V

S

P

K

G

I

Y

A

A

K

S

S

I

F

G

E

V

I

A

-

P

-

L

-

V

I

L

I

A

A

G

S

R

R

L

L

Q

V

G

G

F

I

S

C

A

A

G

G

V

I

E

S

L

S

G

N

G

V

V

V

S

P

V

M

Y

Y

L

L

A

G

E

E

I

L

A

A

K

P

P

K

G

N

K

L

K

R

G

G

F

A

Y

L

V

G

A

V

W

V

Q

P

S
x
Q

A

L

S

F

Q

I

Q

T

V

V

A

G

V

I

I

L

F

V

A

A

G

Q

L

L

L

F

G

G

V

-

L

L

H

R

S

S

M

V

L

L

S

A

P

S

E

E

M

-

G

-

E

-

W

-

G

G

W

W

R

-

I

-

P

P

F

I

L

L

I

L

G

G

C

L

L

T

I

G

V

V

P

P

Q166 (≠ S156) binding ; mutation to E: Impairs D-fructose binding.

Sites not aligning to the query:

31 binding
287 binding
295:297 binding
386 binding
418:419 binding

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
26% identity, 47% coverage: 24:225/433 of query aligns to 54:234/444 of Q8NLB7

query
sites
Q8NLB7

E
x
D

M

G

Y

Y

D
|
D

F

L

M

I

V

V

Y

Y

G

G

F

T

Y

V

A

Q

A

S

A

A

I

L

A

A

K

K

T

-

F

-

P

-

S

-

G

-

N

-

E

E

F

W

A

N

S

L

L

S

M

S

L

A

S

T

L

L

A

G

T

T

F

I

G

G

A

S

-

T

G

A

F

F

L

F

M

G

R

M

P

A

L

I

G

G

A

A

I

V

I

F

L

I

G

G

A

R

Y

L

I

S

D

D

H
x
R

H

V

G

G

R

R

R

K

K

A

G

A

L

V

I

I

M

G

T

S

L

V

V

L

L

I

M

L

A

S

L

V

G

F

T

T

L

M

L

L

I

C

A

A

C

F

V

A

P

P

G

N

Y

-

A

-

S

-

I

-

G

-

V

-

A

-

P

P

L

W

L

V

V

F

L

G

A

A

G

F

R

R

L

F

L

I

Q

A

G

G

F

L

S

G

A

L

G

G

V

G

E

L

L

V

G

P

G

S

V

V

S

N

V

A

Y

M

L

T

A

S

E

D

I

L

A

V

K

-

P

P

G

R

K

K

K

T

G

-

F

-

Y

M

V

S

A

A

W

W

Q

A

S

T

A

V

S

M

Q

M

Q

S

V

-

A

G

V

V

I

P

F

I

A

G

G

G

L

S

L

I

G

A

V

A

V

V

L

L

H

A

S

L

M

V

L

V

S

V

P

P

E

S

M

E

G

-

E

E

W

W

G

G

W

W

R

R

I

F

P

M

F

F

L

L

I

I

G

A

C

-

L

-

I

L

V

I

P

P

F

L

L

V

F

V

L

G

I

L

R

P

R

I

S

A

L

M

E

K

E

V

T

I

E

P

E

S

F

D

K

K

T

A

R

I

K

K

H

A

R

D

P

H

S

D

I

I

S

R

E

E

D54 (≠ E24) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (= D27) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (≠ H79) mutation to A: Loss of transport activity.

Sites not aligning to the query:

309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

Q3T9X0 Solute carrier family 2, facilitated glucose transporter member 9; Glucose transporter type 9; GLUT-9; Urate transporter from Mus musculus (Mouse) (see paper)
24% identity, 58% coverage: 54:305/433 of query aligns to 93:366/538 of Q3T9X0

query
sites
Q3T9X0

L

V

S

T

L

V

A

S

T

I

F

F

G

A

A

I

G

G

F

G

L

L

M

V

R

G

P

T

L

L

G

M

A

V

I

K

I

M

L

I

G

G

A

K

Y

F

I

L

D

-

H

-

G

G

R

R

R

K

K

S

G

T

L

L

I

L

M

V

T

N

L

N

V

G

L

F

M

A

A

I

L

S

G

A

T

A

L

L

I

M

A

A

C

C

V

-

P

-

G

-

Y

-

A

-

S

S

I

L

G

R

V

A

A

G

A

T

P

F

L

E

L

M

V

L

L

I

A

V

G

G

R

R

L

F

L

I

Q

M

G

G

F

V

S

D

A

G

G

G

V

I

E

A

L

L

G

S

G

A

V

L

S

P

V

M

Y

Y

L

L

A

N

E

E

I

I

A

S

K

P

P

K

G

E

K

I

K

R

G

G

F

-

Y

-

V

-

A

-

W

-

Q

-

S

-

A

S

S

L

Q

G

Q

Q

V

V

A

T

V

A

I

I

F

F

A

I

G

-

L

C

L

I

G

G

V

V

V

F

L

S

H

G

S

Q

M

L

L

L

S

G

-

L

P

P

E

E

E

L

M

L

G

G

-

R

E

E

W

S

G

T

W

W

R

-

I

-

P

P

F

Y

L

L

I

F

G

G

C

V

L

I

I

I

V

V

P

P

F

A

L

L

-

V

-

Q

-

L

-

A

-

S

-

L

-

P

-

F

-

L

-

P

-

E

-

S

-

P

-

R

-

Y

-

L

-

F

-

E

-

K

-

H

-

D

-

E

-

A

-

G

-

A

-

M

-

K

-

A

-

F

-

Q

-

T

F

F

L

L

I

G

R

K

R

A

S

D

L

V

-

S

E

Q

E

E

T

L

E

E

-

A

-

L

F

A

K

E

T

S

R

R

K

V

H

Q

R

R

P

N

-

L

-

R

-

L

-

V

S

S

I

V

S

L

E

E

I

L

Y

L

R

R

S

A

M

P

M

F

E

V

N

R

W

W

R

Q

I

V

I

I

V

T

A

V

G

I

C

I

M

T

M

M

V

A

V

S

M

Y

T

Q

T

L

V

C

S

G

F

L

Y

N

M

A

I

I

T

W

A

F

Y

Y

T

T

P

N

T

S

-

I

F

F

G

G

K

K

T

A

V

G

L

I

K

P

L

Q

D

D

D

K

V

I

D

P

N

-

L

-

I

Y

V

I

T

T

M

L

C

S

V

T

G

G

L

G

S

I

N

E

F

T

I

L

W

A

L

A

P

I

L

F

M

S

G

G

A

L

L

V

S

I

D

E

R

R

V

L

G

G

R

R

K

R

P

P

L

L

V

I

Sites not aligning to the query:

71 N→Q: Decreased N-glycosylation.

Q9Y7Q9 Probable metabolite transporter C2H8.02 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
23% identity, 36% coverage: 68:225/433 of query aligns to 98:262/583 of Q9Y7Q9

query
sites
Q9Y7Q9

L

M

G

G

A

Q

I

L

L

F

G

G

A

F

Y

L

I

G

D

D

H

F

G

G

R

R

R

K

K

F

G

V

L

Y

I

G

M

K

T

E

L

M

V

M

L

V

M

V

A

I

L

I

G

A

T

T

L

I

L

L

I

I

A

I

C

C

V

L

P

P

G

D

Y

R

A

I

S

P

I

T

G

P

V

T

A

A

A

K

P

M

L

M

L

W

V

L

L

F

A

A

G

F

R

R

L

V

L

M

Q

L

G

G

F

I

S

G

A

I

G

G

V

G

E

D

L

Y

G

P

G

M

V

S

S

A

V

S

Y

I

L

T

A

S

E

E

I

Q

A

S

K

L

P

I

G

N

K

R

G

G

F

A

Y

L

V

L

A

A

W

W

Q

I

S

F

A

S

S

N

Q

Q

Q

G

V

W

A

G

V

T

I

L

F

-

A

A

G

G

L

C

L

V

G

A

-

T

-

L

V

I

L

L

H

A

S

C

M

F

L

E

S

K

P

P

-

L

E

N

E

D

M

R

G

G

E

E

W

Y

G

T

-

K

-

L

-

N

-

G

-

V

W

W

R

R

I

I

P

Q

F

F

L

G

I

I

G

A

C

L

L

F

I

P

V

A

P

V

F

I

L

V

F

L

L

I

I

P

R

R

R

L

S

R

L

M

E

Q

E

E

T

S

E

E

E

Q

F

F

K

K

T

N

R

S

K

K

H

N

R

M

P

K

S

S

I

P

S

G

E

E

Sites not aligning to the query:

267 modified: Phosphoserine
269 modified: Phosphoserine
289 modified: Phosphoserine
290 modified: Phosphoserine
292 modified: Phosphoserine
330 modified: Phosphoserine

Query Sequence

>RR42_RS07875 FitnessBrowser__Cup4G11:RR42_RS07875
MSSSPSQQHGFRTVFRVVSGNFLEMYDFMVYGFYAAAIAKTFFPSGNEFASLMLSLATFG
AGFLMRPLGAIILGAYIDHHGRRKGLIMTLVLMALGTLLIACVPGYASIGVAAPLLVLAG
RLLQGFSAGVELGGVSVYLAEIAKPGKKGFYVAWQSASQQVAVIFAGLLGVVLHSMLSPE
EMGEWGWRIPFLIGCLIVPFLFLIRRSLEETEEFKTRKHRPSISEIYRSMMENWRIIVAG
CMMVVMTTVSFYMITAYTPTFGKTVLKLDDVDNLIVTMCVGLSNFIWLPLMGALSDRVGR
KPLLVIFTILTLLTAYPAVSWLVAEPSFGRLLMVELWLSFLYGSYNGAMVVTLTEVMPPA
VRTAGFSLAYSLATALFGGFTPAISTYLIHSTGNKAAPGLWLMFAAACGLIATLVIFRGG
RQHAYVEPAAKTA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory