SitesBLAST
Comparing RR42_RS09075 FitnessBrowser__Cup4G11:RR42_RS09075 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 10 hits to proteins with known functional sites (download)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
23% identity, 98% coverage: 3:426/432 of query aligns to 4:360/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (= I6), G8 (= G7), G10 (= G9), V11 (= V10), I12 (= I11), V30 (= V29), E31 (= E30), K32 (≠ R31), E38 (= E38), A39 (≠ S39), S40 (= S40), A43 (≠ N43), G45 (= G45), L46 (≠ V46), V171 (≠ A222), G200 (≠ A264), G201 (= G265), W203 (≠ A267), G298 (= G365), R300 (= R367), P301 (= P368), Y326 (≠ G392), R327 (≠ S393), N328 (≠ T394), G329 (= G395), I330 (≠ W396)
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
29% identity, 37% coverage: 254:413/432 of query aligns to 192:349/369 of S5FMM4
- S202 (≠ A264) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
- I332 (≠ W396) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- M342 (≠ A406) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Sites not aligning to the query:
- 51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- 54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- 81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
27% identity, 45% coverage: 235:427/432 of query aligns to 179:368/368 of 4yshB
- active site: I262 (= I322), L283 (= L343), G305 (= G365), N335 (≠ T394), L338 (≠ A397)
- binding flavin-adenine dinucleotide: S206 (≠ A264), W209 (≠ A267), R307 (= R367), H332 (= H391), R334 (≠ S393), N335 (≠ T394), G336 (= G395), I337 (≠ W396), L338 (≠ A397)
- binding glycine: G249 (vs. gap), Y251 (≠ V311), Y251 (≠ V311), A264 (≠ G324), R307 (= R367), R334 (≠ S393), R334 (≠ S393)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48, 178
Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
27% identity, 45% coverage: 235:427/432 of query aligns to 181:370/377 of Q5L2C2
- R309 (= R367) binding
- 334:340 (vs. 391:397, 29% identical) binding
- R336 (≠ S393) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
- 180 binding
4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
27% identity, 45% coverage: 235:427/432 of query aligns to 179:368/370 of 4yshA
- active site: I262 (= I322), L283 (= L343), G305 (= G365), N335 (≠ T394), L338 (≠ A397)
- binding flavin-adenine dinucleotide: S206 (≠ A264), G207 (= G265), W209 (≠ A267), R307 (= R367), H332 (= H391), R334 (≠ S393), N335 (≠ T394), G336 (= G395), I337 (≠ W396)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48, 178
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
26% identity, 38% coverage: 257:418/432 of query aligns to 195:354/369 of O31616
- H244 (≠ Y309) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (= R367) binding
- 327:333 (vs. 391:397, 29% identical) binding
- R329 (≠ S393) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
- 174 binding
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
26% identity, 38% coverage: 257:418/432 of query aligns to 195:354/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (≠ V311), R302 (= R367), R329 (≠ S393)
- binding flavin-adenine dinucleotide: S202 (≠ A264), G203 (= G265), W205 (≠ A267), F209 (≠ L271), G300 (= G365), R302 (= R367), H327 (= H391), R329 (≠ S393), N330 (≠ T394), G331 (= G395), I332 (≠ W396)
- binding phosphate ion: R254 (= R319)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49, 174
- binding phosphate ion: 89
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
26% identity, 38% coverage: 257:418/432 of query aligns to 195:354/364 of 3if9A
- binding flavin-adenine dinucleotide: S202 (≠ A264), G203 (= G265), W205 (≠ A267), F209 (≠ L271), G300 (= G365), R302 (= R367), H327 (= H391), F328 (≠ G392), R329 (≠ S393), N330 (≠ T394), G331 (= G395), I332 (≠ W396)
- binding glycolic acid: Y246 (≠ V311), R302 (= R367), R329 (≠ S393)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49, 173, 174
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
27% identity, 56% coverage: 177:418/432 of query aligns to 154:374/383 of 5i39A
- active site: Q248 (≠ S288), P267 (≠ T314)
- binding flavin-adenine dinucleotide: A196 (≠ D221), A197 (= A222), G226 (≠ A264), G227 (= G265), W229 (≠ A267), Q248 (≠ S288), Q250 (≠ T290), G321 (= G365), M323 (≠ R367), T348 (= T394), G349 (= G395), W350 (= W396), G351 (≠ A397), M352 (= M398), T353 (≠ A399)
Sites not aligning to the query:
- active site: 66, 69, 70
- binding flavin-adenine dinucleotide: 30, 31, 33, 34, 35, 53, 54, 55, 62, 63, 66, 67, 69
6pxsA Crystal structure of iminodiacetate oxidase (idaa) from chelativorans sp. Bnc1 (see paper)
24% identity, 98% coverage: 1:424/432 of query aligns to 1:365/370 of 6pxsA
- binding flavin-adenine dinucleotide: G7 (= G7), G9 (= G9), I10 (≠ V10), D30 (≠ E30), N32 (≠ S39), H33 (≠ S40), K36 (≠ N43), A37 (= A44), T38 (≠ G45), A40 (≠ I47), G41 (≠ A48), A42 (≠ P49), G43 (= G50), V44 (≠ Y51), Y174 (≠ K232), A203 (= A264), W206 (≠ A267), I210 (≠ L271), Y250 (= Y309), G305 (= G365), R307 (= R367), G333 (= G392), A334 (≠ S393), S335 (≠ T394), G336 (= G395), L337 (≠ W396), T338 (≠ A397)
Query Sequence
>RR42_RS09075 FitnessBrowser__Cup4G11:RR42_RS09075
MHVIVIGAGVIGVCSAWYLREAGFDVTVVERRAAPAQESSFGNAGVIAPGYVTPWAAPGM
PGKVLRNLFASTSPVLFRPSADPAMWRWIARWLGECTLERYRLNKLRMQRLAFYSRQCLH
ALRGQLQIDYEQSQGYLQLFRTQRDRDLAEPALALLREHKVAHRLVDADGCRRIEPGLTT
DTPLAGGLHLPEDESGNCPMFVRRLRVLAEAAGVRFVMDSDASALRPLPGGKLSLDLRST
AAGDARSARGVRETLTADRVLVSAGIASAALLRPLGLRIPLYPVKGYSATVHVSDELQAP
LGALMDESYKVAITRMGNRLRIAGTAELGSRKLDLRPAAINTLLKVARDWFPVAGHYGTA
TLWAGARPMLPDGPPLIGATAVPGLYLNLGHGSTGWAMACGSGRIAADLIAGNRPGIDLD
GLTPDRYGLGPR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory