SitesBLAST

Q9BTZ2 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase; CR; NADPH-dependent retinol dehydrogenase/reductase; NRDR; humNRDR; Peroxisomal short-chain alcohol dehydrogenase; PSCD; SCAD-SRL; Short chain dehydrogenase/reductase family 25C member 2; Protein SDR25C2; Short-chain dehydrogenase/reductase family member 4; EC 1.1.1.184 from Homo sapiens (Human) (see 2 papers)
30% identity, 96% coverage: 6:247/253 of query aligns to 36:272/278 of Q9BTZ2

query
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Q9BTZ2

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S176 (≠ L142) Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin; mutation to F: Decreased reduction activity for benzil, isatin and retinal and increased activity for 5beta-Pregnane-3,20-dione and 5beta-Dihydrotestosterone. No change of stereoselectivity in 3-ketosteroids reduction and no change in 3beta-hydroxysteroid oxidation. Decreased reduction activity for isatin and increased activity for 5beta-Pregnane-3,20-dione, 5beta-Dihydrotestosterone, benzil and retinal; when associated with L-179. Change in stereoselective activity by the reduction of 5beta-Pregnane-3,20-dione predominantly to the 3alpha-hydroxysteroid; when associated with L-179. Switch from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with L-179. Loss of cold catalytic inactivation; when associated with L-179 and N-195. Increased reduction activity for renital and oxidation activity for retinol; when associated with L-179 and N-195.
F179 (≠ R145) Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin; mutation to L: Decreased reduction activity for isatin and increased activity for 5beta-Pregnane-3,20-dione, 5beta-Dihydrotestosterone, benzil and retinal; when associated with F-176. Change in stereoselective activity by the reduction of 5beta-Pregnane-3,20-dione predominantly to the 3alpha-hydroxysteroid; when associated with F-176. Switch from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with F-176. Loss of cold catalytic inactivation; when associated with F-176 and N-195. Increased reduction activity for renital and oxidation activity for retinol; when associated with F-176 and N-195.
T195 (≠ N161) mutation to N: Loss of cold catalytic inactivation. Loss of cold catalytic inactivation; when associated with F-176 and L-179. Switch in stereoselective activity from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with F-176 and L-179. Increased reduction activity for renital and oxidation activity for retinol; when associated with F-176 and L-179.

Q8WNV7 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; Peroxisomal carbonyl reductase; PerCR; Peroxisomal short-chain alcohol dehydrogenase; PSCD; Short chain dehydrogenase/reductase family 25C member 2; Protein SDR25C2; EC 1.1.1.184; EC 1.1.1.300 from Sus scrofa (Pig) (see 2 papers)
32% identity, 98% coverage: 1:247/253 of query aligns to 31:273/279 of Q8WNV7

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Q8WNV7

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37:61 (vs. 6:30, 36% identical) binding
F177 (≠ L142) Responsible for the stereoselective reduction of 3-ketosteroids into 3alpha-hydroxysteroids and benzil into S-benzoin; mutation to S: Change in stereoselective activity by the reduction of 3-ketosteroids and benzil into 3beta-hydroxysteroid and R-benzoin, respectively; when associated with F-180.
L180 (≠ R145) Responsible for the stereoselective reduction of 3-ketosteroids into 3alpha-hydroxysteroids and benzil into S-benzoin; mutation to F: Change in stereoselective activity by the reduction of 3-ketosteroids and benzil into 3beta-hydroxysteroid and R-benzoin, respectively; when associated with S-177.
Y183 (= Y148) active site, Proton acceptor
K187 (= K152) binding
N196 (= N161) Important for the maintenance of the quaternary structure, the catalytic activity and cold stability

Sites not aligning to the query:

277:279 Peroxisomal targeting signal

3o4rA Crystal structure of human dehydrogenase/reductase (sdr family) member 4 (dhrs4)
30% identity, 96% coverage: 6:247/253 of query aligns to 12:248/254 of 3o4rA

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3o4rA

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active site: G19 (≠ S13), S145 (≠ T135), F155 (≠ R145), Y158 (= Y148), K162 (= K152), K203 (≠ A202)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A15 (≠ G9), T17 (≠ A11), D18 (= D12), G19 (≠ S13), I20 (≠ V14), S39 (≠ D33), R40 (≠ V34), K41 (≠ R35), N44 (≠ A38), H65 (≠ N55), V66 (= V56), N92 (= N82), A94 (≠ G84), S145 (≠ T135), Y158 (= Y148), K162 (= K152), P188 (= P178), G189 (= G179), L190 (≠ M180), I191 (= I181), T193 (≠ N183), F195 (≠ M186), S196 (≠ G187)

4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
32% identity, 98% coverage: 1:247/253 of query aligns to 3:235/239 of 4nbtA

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4nbtA

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active site: G16 (≠ S13), S132 (≠ T135), Y145 (= Y148), K149 (= K152)
binding nicotinamide-adenine-dinucleotide: G12 (= G9), K15 (≠ D12), G16 (≠ S13), L17 (≠ V14), D36 (= D33), L37 (= L39), L52 (≠ A54), N53 (= N55), V54 (= V56), N80 (= N82), A81 (≠ V83), G82 (= G84), I130 (≠ F133), S132 (≠ T135), Y145 (= Y148), K149 (= K152), P177 (= P178), G178 (= G179), I180 (= I181), T182 (≠ G187)

3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
33% identity, 96% coverage: 6:247/253 of query aligns to 5:235/239 of 3sj7A

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E

E

A

E

T

I

L

K

A

A

A

K

N

G

P

V

G

D

M

S

T

F

G

A

T

I

V

Q

A
|
A

N
|
N

V
|
V

G

A

D

D

P

A

A

D

G

E

V

V

A

K

R

A

V

M

V

I

Q

K

D

E

A

V

E

V

R

S

V

Q

F

F

G

G

D

S

V

L

S

D

V

V

L

L

V

V

N

N

N
|
N

V
x
A

G

G

I

I

A

T

G

R

P

D

R

N

A

L

A

-

L

L

E

M

D

R

I

M

S

K

D

E

D

Q

D

E

W

W

R

D

D

D

T

V

M

I

E

D

V
x
T

N

N

V

L

G

K

A

G

M

V

F

F

Q

N

F

C

M

I

K

Q

R

K

V

A

V

T

P

P

S

Q

M

M

K

L

R

R

N

Q

R

R

H

S

G

G

V

A

I

I

N

N

F

L

S

S

T
x
S

G

V

S

V

T

G

R

A

T

V

R

G

L

N

P

P

M

G

R
x
Q

T

A

P

N

Y
|
Y

V

V

V

A

S

T

K
|
K

A

A

A

G

V

V

E

I

S

G

L

L

T

T

L

K

N

S

A

A

R

R

E

E

L

L

G

A

P

S

F

R

N

G

V

I

R

T

C

V

N

N

A

A

I

V

L

A

P
|
P

G
|
G

M

F

I

-

D

-

N

-

E

-

R

-

M

-

G

-

R

-

I

I

V

V

A

S

G

D

I

M

A

T

H

D

E

E

S

-

G

-

R

-

S

-

A

-

D

-

V

L

E

K

A

E

D

Q

Y

M

L

L

K

T

Y

Q

I

I

S

P

L

L

R

A

C

R

K

F

V

G

Q

Q

P

D

D

T

D

D

L

I

A

A

Q

N

T

T

V

V

L

A

F

F

L

L

A

A

S

S

G

D

A

K

A

A

S

K

K

Y

I

I

T

T

G

G

E

Q

L

T

I

I

A

H

V

V

S

N

G

G

active site: G12 (≠ S13), S138 (≠ T135), Q148 (≠ R145), Y151 (= Y148), K155 (= K152)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (≠ A11), R11 (≠ D12), I13 (≠ V14), N31 (vs. gap), Y32 (vs. gap), A33 (vs. gap), G34 (vs. gap), S35 (≠ C32), A58 (= A54), N59 (= N55), V60 (= V56), N86 (= N82), A87 (≠ V83), T109 (≠ V106), S138 (≠ T135), Y151 (= Y148), K155 (= K152), P181 (= P178), G182 (= G179)

8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
32% identity, 95% coverage: 6:245/253 of query aligns to 9:244/251 of 8cxaA

query
sites
8cxaA

I

I

V

I

T

T

G
|
G

G

A

A

A

D
x
Q

S
x
G

V
x
I

G

G

R

R

V

I

I

Y

A

A

E

E

Q

R

F

F

R

A

A

E

S

E

G

G

A

A

R

A

V

V

H

V

I

V

C

A

D
|
D

V

I

-

N

-

E

-

P

R

K

A

A

D

T

A

E

L

V

E

A

A

S

T

S

L

I

A

T

A

S

N

L

P

G

G

G

M

R

T

A

G

I

T

A

V

A

A

A

-

V

N

D

V
|
V

G

S

D

D

P

V

A

E

G

S

V

V

A

N

R

R

V

M

V

V

Q

D

D

S

A

A

E

V

R

E

V

A

F

F

G

G

D

T

V

V

S

D

V

I

L

L

V

V

N

N

N
|
N

V

A

G
x
A

I

I

A

F

G
x
S

-

T

-

L

P

K

R

M

A

K

A

P

L

F

E

E

D

E

I

I

S

S

D

G

D

D

D

E

W

W

R

D

D

K

T

L

M

F

E

A

V

V

N

N

V

A

G

K

A

G

M

T

F

F

Q

L

F

C

M

C

K

Q

R

A

V

V

V

S

P

P

S

V

M

M

K

R

R

K

N

N

R

Q

H

Y

G

G

V

R

I

I

N

N

F
x
I

S
|
S

T
x
S

G

S

S

V

T

V

R

V

T

T

R

G

L

R

P

P

M

N

R

Y

T

A

P

H

Y
|
Y

V

I

V

A

S

S

K
|
K

A

G

A

A

V

V

E

W

S

A

L

L

T

T

L

H

N

A

A

L

A

A

R

T

E

E

L

M

G

G

P

T

F

D

N

G

V

I

R

T

C

V

N

N

A

T

I

V

L

S

P
|
P

G
x
H

M

G

I
|
I

D

I

N

T

E

E

R

-

M
x
I

G

P

R

R

I

-

V

-

A

E

G

T

I

I

A

S

H

E

E

E

S

G

G

W

R

R

S

R

A

N

A

L

D

E

V

E

E

Q

A

A

D

-

Y

-

L

-

K

-

Y

-

I

-

S

-

L

L

R

K

C

R

K

K

V

G

Q

D

P

A

D

S

D

D

L

L

A

V

Q

G

T

I

V

L

L

L

F

Y

L

L

A

A

S

S

G

D

A

E

A

S

S

K

K

F

I

M

T

T

G

G

E

Q

L

T

I

V

A

A

V

L

binding nicotinamide-adenine-dinucleotide: G12 (= G9), Q15 (≠ D12), G16 (≠ S13), I17 (≠ V14), D36 (= D33), V63 (= V56), N89 (= N82), A91 (≠ G84), S94 (≠ G87), I142 (≠ F133), S143 (= S134), S144 (≠ T135), Y157 (= Y148), K161 (= K152), P187 (= P178), H188 (≠ G179), I190 (= I181), I194 (≠ M186)

1ahhA 7 alpha-hydroxysteroid dehydrogenase complexed with NAD+ (see paper)
30% identity, 96% coverage: 6:247/253 of query aligns to 15:248/253 of 1ahhA

query
sites
1ahhA

I

I

V

I

T

T

G
|
G

G

A

A

G

D
x
A

S
x
G

V

I

G

G

R

K

V

E

I

I

A

A

E

I

Q

T

F

F

R

A

A

T

S

A

G

G

A

A

R

S

V

V

H

V

I

V

C

S

D
|
D

V
x
I

R

N

A

A

D

D

A

A

L

A

E

N

A

H

T

V

L

V

A

D

A

E

N

I

P

Q

G

Q

M

L

T

G

G

G

T

Q

V

A

-

F

-

A

-

C

-

R

A
x
C

N
x
D

V
x
I

G

T

D

S

P

E

A

Q

G

E

V

L

A

S

R

A

V

L

V

A

Q

D

D

F

A

A

E

I

R

S

V

K

F

L

G

G

D

K

V

V

S

D

V

I

L

L

V

V

N

N

N
|
N

V

A

G
|
G

I

G

A

G

G

G

P

P

R

K

A

P

A

F

L

-

E

-

D

D

I

M

S

P

D

M

D

A

D

D

W

F

R

R

D

R

T

A

M

Y

E

E

V

L

N

N

V

V

G

F

A

S

M

F

F

F

Q

H

F

L

M

S

K

Q

R

L

V

V

V

A

P

P

S

E

M

M

K

E

R

K

N

N

R

G

H

G

G

G

V

V

I

I

I

L

N

T

F

I

S
x
T

T
x
S

G

M

S

A

T

A

R

E

T

N

R

K

L

N

P

I

M

N

R
x
M

T

T

P

S

Y
|
Y

V

A

V

S

S

S

K
|
K

A

A

V

A

E

S

S

H

L

L

T

V

L

R

N

N

A

M

A

A

R

F

E

D

L

L

G

G

P

E

F

K

N

N

V

I

R

R

C

V

N

N

A

G

I

I

L

A

P
|
P

G
|
G

M

A

I
|
I

D

L

N

T

E

D

R

A

M

L

G

K

R

S

I

V

V

I

A

-

G

-

I

-

A

-

H

-

E

-

S

-

G

-

R

-

S

-

A

T

A

P

D

E

V

I

E

E

A

Q

D

K

Y

M

L

L

K

Q

Y

H

I

T

S

P

L

I

R

R

C

R

K

L

V

G

Q

Q

P

P

D

Q

D

D

L

I

A

A

Q

N

T

A

V

A

L

L

F

F

L

L

A

C

S

S

G

P

A

A

S

S

K

W

I

V

T

S

G

G

E

Q

L

I

I

L

A

T

V

V

S

S

G

G

active site: G22 (≠ S13), S146 (≠ T135), M156 (≠ R145), Y159 (= Y148), K163 (= K152)
binding nicotinamide-adenine-dinucleotide: G18 (= G9), A21 (≠ D12), D42 (= D33), I43 (≠ V34), C67 (≠ A54), D68 (≠ N55), I69 (≠ V56), N95 (= N82), G97 (= G84), T145 (≠ S134), Y159 (= Y148), K163 (= K152), P189 (= P178), G190 (= G179), I192 (= I181)

1ahiA 7 alpha-hydroxysteroid dehydrogenase complexed with nadh and 7-oxo glycochenodeoxycholic acid (see paper)
30% identity, 96% coverage: 6:247/253 of query aligns to 15:248/255 of 1ahiA

query
sites
1ahiA

I

I

V

I

T

T

G
|
G

G

A

A

G

D
x
A

S
x
G

V
x
I

G

G

R

K

V

E

I

I

A

A

E

I

Q

T

F

F

R

A

A

T

S

A

G

G

A

A

R

S

V

V

H

V

I

V

C

S

D
|
D

V
x
I

R

N

A

A

D

D

A

A

L

A

E

N

A

H

T

V

L

V

A

D

A

E

N

I

P

Q

G

Q

M

L

T

G

G

G

T

Q

V

A

-

F

-

A

-

C

-

R

A

C

N
x
D

V
x
I

G

T

D

S

P

E

A

Q

G

E

V

L

A

S

R

A

V

L

V

A

Q

D

D

F

A

A

E

I

R

S

V

K

F

L

G

G

D

K

V

V

S

D

V

I

L

L

V

V

N

N

N
|
N

V

A

G

G

I

G

A

G

G

G

P

P

R

K

A

P

A

F

L

-

E

-

D

D

I

M

S

P

D

M

D

A

D

D

W

F

R

R

D

R

T

A

M

Y

E

E

V

L

N

N

V

V

G

F

A

S