SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS11480 FitnessBrowser__Cup4G11:RR42_RS11480 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
49% identity, 73% coverage: 28:104/105 of query aligns to 4:80/81 of 1a56A

query
sites
1a56A

L

L

A

A

D

K

K

K

S

N

G
x
N

C
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C

F

I

S

A

C
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C

H
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H

G

Q

M

V

K

E

T

T

K

K

V

V

V
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V

G
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G

P
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P

G

A

F

L

V

K

E

D

V

I

A

A

G

A

K

K

Y

Y

K

A

G

D

D

K

G

D

E

D

A

A

P

A

E

T

R

Y

L

L

A

A

K

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K
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K

I
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I

R

K

E

G

G

G

G
x
S

K

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W
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W

G

G

R
x
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I
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I

P

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M

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P

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x
N

D
x
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N

L

V

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S

E

D

T

A

D

D

A

A

R

K

K

A

L

L

A

A

E

D

W

W

V

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L

L

S

T

I

L

binding heme c: N9 (≠ G33), C10 (= C34), C13 (= C37), H14 (= H38), V21 (= V45), G22 (= G46), P23 (= P47), K45 (= K69), I46 (= I70), S50 (≠ G74), W54 (= W78), Q56 (≠ R80), I57 (= I81), M59 (= M83), N62 (≠ H86), V63 (≠ D87), N64 (= N88)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
48% identity, 77% coverage: 24:104/105 of query aligns to 2:81/82 of 5xecA

query
sites
5xecA

D

D

M

P

Q

E

A

V

L

L

A

F

D

K

K

N

S

K

G

G

C
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C

F

V

S

A

C
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C

H
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H

G

A

M

I

K

D

T

T

K

K

V

K

V

V

G
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G

P
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P

G

A

F
x
Y

V

A

E

D

V

V

A

A

G

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K

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Y
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Y

K

A

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G

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R

G

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A

A

P

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E

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R
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Y

L

L

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A

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I
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I

R

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K

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G

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S

G
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G

V
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V

W
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W

G
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G

R

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I
x
V

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M
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M

P

P

H
x
Q

D

-

N

N

L

V

S

T

E

D

T

A

D

E

A

A

R

K

K

Q

L

L

A

A

E

Q

W

W

V
x
I

L

L

S

S

I

I

binding heme c: C12 (= C34), C15 (= C37), H16 (= H38), G24 (= G46), P25 (= P47), Y27 (≠ F49), Y34 (= Y56), Y43 (≠ R65), I48 (= I70), S53 (≠ K75), G54 (= G76), V55 (= V77), W56 (= W78), G57 (= G79), V59 (≠ I81), M61 (= M83), Q64 (≠ H86), I78 (≠ V101)

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
48% identity, 75% coverage: 26:104/105 of query aligns to 2:82/83 of 5aurA

query
sites
5aurA

Q

E

A

Q

L

L

A

A

D

K

K

Q

S

K

G

G

C
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C

F

M

S

A

C
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C

H
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H

G

D

M

L

K

K

T

A

-

G

K

K

V

K

V

V

G

G

P
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P

G

A

F
x
Y

V

A

E

D

V

V

A

A

G

K

K

K

Y
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Y

K

A

G

G

D

R

G

K

E

D

A

A

P

V

E

D

R
x
Y

L

L

A

A

K

G

K

K

I
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I

R

K

E

K

G

G

K
x
S

G
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G

V
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V

W
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W

G
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G

R

S

I
x
V

P
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P

M
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M

P

P

H
x
Q

D

-

N

N

L

V

S

T

E

D

T

A

D

E

A

A

R

K

K

Q

L

L

A

A

E

Q

W

W

V

I

L

L

S

S

I

I

binding heme c: C10 (= C34), C13 (= C37), H14 (= H38), P26 (= P47), Y28 (≠ F49), Y35 (= Y56), Y44 (≠ R65), I49 (= I70), S54 (≠ K75), G55 (= G76), V56 (= V77), W57 (= W78), G58 (= G79), V60 (≠ I81), P61 (= P82), M62 (= M83), Q65 (≠ H86)

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
48% identity, 76% coverage: 24:103/105 of query aligns to 2:80/82 of 2exvA

query
sites
2exvA

D

D

M

P

Q

E

A

V

L

L

A

A

D

K

K

N

S

K

G

G

C
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C

F

V

S

A

C
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C

H
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H

G

A

M

I

K

D

T

T

K

K

V

M

V

V

G
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G

P
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P

G

A

F

Y

V

K

E

D

V

V

A

A

G

A

K

K

Y

F

K

A

G

G

D

Q

G

A

E

G

A

A

P

E

E

A

R

E

L

L

A

A

K

Q

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x
R

I
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I

R

K

E

N

G

G

G
x
S

K
x
Q

G
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G

V
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V

W
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W

G
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G

R

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I
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I

P
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P

M
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M

P

P

H

-

D
x
N

N

A

L

V

S

S

E

D

T

D

D

E

A

A

R

Q

K

T

L

L

A

A

E

K

W

W

V

V

L

L

S

S

binding heme c: C12 (= C34), C15 (= C37), H16 (= H38), G24 (= G46), P25 (= P47), R47 (≠ K69), I48 (= I70), S52 (≠ G74), Q53 (≠ K75), G54 (= G76), V55 (= V77), W56 (= W78), G57 (= G79), I59 (= I81), P60 (= P82), M61 (= M83), N64 (≠ D87)

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
47% identity, 74% coverage: 26:103/105 of query aligns to 2:78/80 of 5xecC

query
sites
5xecC

Q

E

A

Q

L

L

A

A

D

K

K

Q

S

K

G

G

C
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C

F

M

S

A

C
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C

H
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H

G

D

M

L

K

K

T

A

K

K

V

M

V

V

G

G

P
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P

G

A

F
x
Y

V

K

E

D

V

V

A

A

G

A

K

K

Y

F

K

A

G

G

D

Q

G

A

E

G

A

A

P

E

E

A

R

E

L
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L

A

A

K

Q

K
x
R

I

I

R

K

E

N

G

G

G

S

K
x
Q

G

G

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V

W
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W

G
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G

R

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I
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I

P

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M
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M

P

P

H

-

D
x
N

N

A

L

V

S

S

E

D

T

D

D

E

A

A

R

Q

K

T

L

L

A

A

E

K

W

W

V

V

L

L

S

S

binding heme c: C10 (= C34), C13 (= C37), H14 (= H38), P23 (= P47), Y25 (≠ F49), L42 (= L66), R45 (≠ K69), Q51 (≠ K75), V53 (= V77), W54 (= W78), G55 (= G79), I57 (= I81), M59 (= M83), N62 (≠ D87)

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
46% identity, 78% coverage: 22:103/105 of query aligns to 22:102/104 of P00099

query
sites
P00099

A
|
A

P
x
E

D
|
D

M
x
P

Q
x
E

A
x
V

L
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L

A
x
F

D
x
K

K
x
N

S
x
K

G
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G

C
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C

F
x
V

S
x
A

C
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C

H
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H

G
x
A

M
x
I

K
x
D

T
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T

K
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K

V
x
M

V
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V

G
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G

P
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P

G
x
A

F
x
Y

V
x
K

E
x
D

V
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V

A
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A

G
x
A

K
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K

Y
x
F

K
x
A

G
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G

D
x
Q

G
x
A

E
x
G

A
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A

P
x
E

E
x
A

R
x
E

L
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L

A
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A

K
x
Q

K
x
R

I
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I

R
x
K

E
x
N

G
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G

G
x
S

K
x
Q

G
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G

V
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V

W
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W

G
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G

R
x
P

I
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I

P
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P

M
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M

P
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P

H

-

D
x
N

N
x
A

L
x
V

S
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S

E
x
D

T
x
D

D
x
E

A
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A

R
x
Q

K
x
T

L
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L

A
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A

E
x
K

W
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W

V
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V

L
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L

S
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S

H38 (= H38) binding axial binding residue
M83 (= M83) binding axial binding residue

Sites not aligning to the query:

1:22 signal peptide
23:104 modified: mature protein, Cytochrome c-551

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
46% identity, 76% coverage: 24:103/105 of query aligns to 2:80/82 of 351cA

query
sites
351cA

D

D

M

P

Q

E

A

V

L

L

A

F

D

K

K

N

S

K

G

G

C
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C

F

V

S

A

C
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C

H
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H

G

A

M

I

K

D

T

T

K

K

V

M

V

V

G
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G

P
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P

G

A

F
x
Y

V

K

E

D

V

V

A

A

G

A

K

K

Y

F

K

A

G

G

D

Q

G

A

E

G

A

A

P

E

E

A

R

E

L
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L

A

A

K

Q

K
x
R

I
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I

R

K

E

N

G

G

G

S

K
x
Q

G
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G

V
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V

W
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W

G
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G

R

P

I
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I

P
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P

M
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M

P

P

H

-

D

N

N

A

L

V

S

S

E

D

T

D

D

E

A

A

R

Q

K

T

L

L

A

A

E

K

W

W

V

V

L

L

S

S

binding protoporphyrin ix containing fe: C12 (= C34), C15 (= C37), H16 (= H38), G24 (= G46), P25 (= P47), Y27 (≠ F49), L44 (= L66), R47 (≠ K69), I48 (= I70), Q53 (≠ K75), G54 (= G76), V55 (= V77), W56 (= W78), G57 (= G79), I59 (= I81), P60 (= P82), M61 (= M83)

2d0sA Crystal structure of the cytochrome c552 from moderate thermophilic bacterium, hydrogenophilus thermoluteolus (see paper)
44% identity, 75% coverage: 26:104/105 of query aligns to 2:78/79 of 2d0sA

query
sites
2d0sA

Q

E

A

A

L

L

A

A

D

K

K

A

S

K

G

G

C
|
C

F

M

S

A

C
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C

H
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H

G

A

M

I

K

D

T

K

K

K

V

L

V

V

G

G

P
|
P

G

S

F

Y

V

K

E

D

V

V

A

A

G

K

K

K

Y
|
Y

K

-

G

T

D

E

G

A

E

D

A

V

P

P

E

-

R

K

L

L

A

V

K

E

K
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K

I
x
V

R

K

E

K

G

G

K
x
A

G
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G

V
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V

W
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W

G
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G

R

P

I
x
V

P

P

M
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M

P
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P

P

P

H
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H

D

P

N

Q

L

V

S

A

E

E

T

A

D

D

A

I

R

E

K

K

L

I

A

V

E

R

W

W

V

V

L

L

S

T

I

L

binding heme c: C10 (= C34), C13 (= C37), H14 (= H38), P23 (= P47), Y32 (= Y56), K43 (= K69), V44 (≠ I70), A49 (≠ K75), G50 (= G76), V51 (= V77), W52 (= W78), G53 (= G79), V55 (≠ I81), M57 (= M83), P58 (= P84), H60 (= H86)

1corA Investigation of the solution conformation of cytochromE C-551 from pseudomonas stutzeri (see paper)
49% identity, 76% coverage: 24:103/105 of query aligns to 2:80/82 of 1corA

query
sites
1corA

D

D

M

G

Q

E

A

A

L

L

A

F

D

K

K

S

S
x
K

G

P

C
|
C

F

A

S

A

C
|
C

H
|
H

G

S

M

I

K

D

T

A

K

K

V

L

V
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V

G
|
G

P
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P

G

A

F

F

V

K

E

E

V

V

A

A

G

A

K

K

Y

Y

K

A

G

G

D

Q

G

D

E

G

A

A

P

A

E

D

R

L

L
|
L

A

A

K

G

K
x
H

I
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I

R

K

E

N

G

G

G
x
S

K
x
Q

G
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G

V
|
V

W
|
W

G
|
G

R

P

I
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I

P

P

M
|
M

P

P

H

-

D
x
N

N

P

L

V

S

T

E

E

T

E

D

E

A

A

R

K

K

I

L

L

A

A

E

E

W

W

V

I

L

L

S

S

binding heme c: K10 (≠ S32), C12 (= C34), C15 (= C37), H16 (= H38), V23 (= V45), G24 (= G46), P25 (= P47), L44 (= L66), H47 (≠ K69), I48 (= I70), S52 (≠ G74), Q53 (≠ K75), G54 (= G76), V55 (= V77), W56 (= W78), G57 (= G79), I59 (= I81), M61 (= M83), N64 (≠ D87)

6kq1A Crystal structure of cytochrome c551 from pseudomonas sp. Strain mt-1.
47% identity, 77% coverage: 24:104/105 of query aligns to 2:81/82 of 6kq1A

query
sites
6kq1A

D
|
D

M

G

Q
x
E

A

A

L

L

A

F

D

K

K

S

S
x
K

G

P

C
|
C

F

A

S

A

C
|
C

H
|
H

G

S

M

I

K

D

T

A

K

K

V

M

V

V

G
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G

P
|
P

G

A

F

L

V

K

E

E

V
|
V

A

A

G

A

K

K

Y
|
Y

K

A

G

G

D

Q

G

E

E

G

A

A

P

A

E

D

R

L

L
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L

A

A

K

G

K
x
H

I
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I

R

K

E

N

G

G

G

T

K
x
Q

G
|
G

V
x
N

W
|
W

G
|
G

R

P

I
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I

P

P

M
|
M

P

P

H

-

D
x
N

N

P

L

V

S

T

E

E

T

E

D
x
E

A

A

R

K

K

T

L

L

A

A

E

E

W

W

V

V

L

L

S

S

I

L

binding heme c: C12 (= C34), C15 (= C37), H16 (= H38), G24 (= G46), P25 (= P47), V30 (= V52), Y34 (= Y56), L44 (= L66), H47 (≠ K69), I48 (= I70), Q53 (≠ K75), G54 (= G76), N55 (≠ V77), W56 (= W78), G57 (= G79), I59 (= I81), M61 (= M83), N64 (≠ D87)
binding zinc ion: D2 (= D24), E4 (≠ Q26), K10 (≠ S32), E70 (≠ D93)

1cchA The solution conformation of cytochromE C-551 from p.Stutzeri zobell determined by nmr+ (see paper)
48% identity, 77% coverage: 24:104/105 of query aligns to 2:81/82 of 1cchA

query
sites
1cchA

D

D

M

G

Q

E

A

A

L

L

A

F

D

K

K

S

S

K

G

P

C
|
C

F

A

S

A

C
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C

H
|
H

G

S

M

V

K

D

T

T

K

K

V

M

V

V

G

G

P

P

G

A

F

L

V

K

E

E

V

V

A

A

G

A

K

K

Y

N

K

A

G

G

D

V

G

E

E

G

A

A

P

A

E

D

R

T

L
|
L

A

A

K

L

K
x
H

I
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I

R

K

E

N

G

G

G
x
S

K
x
Q

G

G

V
|
V

W
|
W

G
|
G

R

P

I
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I

P

P

M
|
M

P
|
P

P

P

H

-

D
x
N

N

P

L

V

S

T

E

E

T

E

D

E

A

A

R

K

K

I

L

L

A

A

E

E

W

W

V

V

L

L

S

S

I

L

binding heme c: C12 (= C34), C15 (= C37), H16 (= H38), L44 (= L66), H47 (≠ K69), I48 (= I70), S52 (≠ G74), Q53 (≠ K75), V55 (= V77), W56 (= W78), G57 (= G79), I59 (= I81), M61 (= M83), P62 (= P84), N64 (≠ D87)

4xxlA Crystal structure of class 1 cytochrome mtod from sideroxydans lithotrophicus es-1 (see paper)
31% identity, 85% coverage: 16:104/105 of query aligns to 1:90/92 of 4xxlA

query
sites
4xxlA

A

A

A

V

R

D

V

V

E

D

A

A

P

-

D

-

M

-

Q

K

A

S

L

L

A

A

D

R

K

E

S

N

G

N

C
|
C

F

F

S

K

C
|
C

H
|
H

G

G

M

V

K

D

T

K

K

E

V

K

V

D

G
|
G

P
|
P

G

S

F
x
Y

V

K

E

K

V

V

A

A

G

E

K
|
K

Y
|
Y

K

R

G

G

D

K

G

A

E

D

A

A

P

E

E

A

R

K

L

L

A

I

K

H

K
x
H

I

V

R

T

E

S

G

G

G

E

K

K

G
x
A

V
x
K

W

F

G

P

R

D

I

G

P

H

M

E

P

E

H
|
H

D

K

N

N

L

I

-

N

-

G

-

K

-

A

S

S

E

P

T

E

D

A

A

I

R

K

K

N

L

L

A

V

E

D

W

W

V

I

L

L

S

S

I

L

binding heme c: C16 (= C34), C19 (= C37), H20 (= H38), G28 (= G46), P29 (= P47), Y31 (≠ F49), K37 (= K55), Y38 (= Y56), H51 (≠ K69), A58 (≠ G76), K59 (≠ V77), H68 (= H86)

Query Sequence

>RR42_RS11480 FitnessBrowser__Cup4G11:RR42_RS11480
MPLTGTVVVVTMMLCAARVEAAPDMQALADKSGCFSCHGMKTKVVGPGFVEVAGKYKGDG
EAPERLAKKIREGGKGVWGRIPMPPHDNLSETDARKLAEWVLSIG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory