SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS11675 FitnessBrowser__Cup4G11:RR42_RS11675 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q58761 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
29% identity, 93% coverage: 11:287/298 of query aligns to 9:282/284 of Q58761

query
sites
Q58761

N

S

E

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R

N

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A

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D

DNE 34:36 (≠ DGE 36:38) binding
D212 (= D218) binding
STGGT 216:220 (≠ ATGST 222:226) binding

1u9zA Crystal structure of phosphoribosyl diphosphate synthase complexed with amp and ribose 5-phosphate (see paper)
29% identity, 93% coverage: 12:287/298 of query aligns to 10:272/274 of 1u9zA

query
sites
1u9zA

E

Q

R

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D

binding adenosine monophosphate: F32 (= F34), D34 (= D36), E36 (= E38), R92 (= R94), Q93 (= Q95), F97 (= F99), H125 (= H127)
binding ribose-5-phosphate: D163 (= D168), D202 (= D218), I205 (≠ V221), S206 (≠ A222), T207 (= T223), G208 (= G224), T210 (= T226)

Q97Z86 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
29% identity, 83% coverage: 34:279/298 of query aligns to 32:278/291 of Q97Z86

query
sites
Q97Z86

F

F

A

P

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D

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G

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E

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I

STGGT 220:224 (≠ ATGST 222:226) binding

4twbA Sulfolobus solfataricus ribose-phosphate pyrophosphokinase (see paper)
27% identity, 83% coverage: 34:279/298 of query aligns to 32:265/278 of 4twbA

query
sites
4twbA

F
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F

A

P

D
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D

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E

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binding adenosine monophosphate: F32 (= F34), D34 (= D36), E36 (= E38), R93 (= R94), F98 (= F99), H127 (= H127)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
29% identity, 97% coverage: 5:294/298 of query aligns to 4:297/307 of 7xmvA

query
sites
7xmvA

L

L

F

F

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G

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A

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L

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F

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L

-

L

A

E

S

D

W

M

L

L

R

Q

A

L

T

N

V

L

N

D

Q

N

P

P

F

I

L

V

V

V

G

S

P

P

D

D

E

-

S

-

R

-

Q

-

W

-

V

I

N

G

D

G

V

V

A
x
V

R

R

R

-

C

A

Q

R

A
|
A

P

I

A

A

I

K

V

L

L

L

H

N

K

D

T

T

R

D

H

M

A

A

-

I

D

D

R

K

C

R

V

R

E

Q

I

V

Q

M

A

H

A

I

D

-

L

I

N

G

A

D

A

V

A

A

G

G

C

R

T

D

P

C

V

V

L

L

L

V

D
|
D

I
x
M

V

I

A
x
D

T
|
T

G

G

S
x
G

T
|
T

M

L

V

C

A

K

A

A

N

E

L

A

L

L

R

K

R

E

A

R

G

G

L

A

A

K

P

R

P

V

V

F

C

A

V

Y

A

A

V

T

H

H

A

P

V

I

F

F

A

S

G

G

D

N

A

A

F

A

A

N

E

N

L

L

R

R

-

N

A

S

V

V

A

I

A

D

E

E

I

V

V

V

T

V

C

C

D

D

T

T

V

I

S

P

H

L

S

S

S

D

N

E

-

I

-

K

-

S

-

L

-

P

-

N

-

V

-

R

G

T

I

L

A

T

L

L

A

S

A

G

P

M

L

L

A

A

E

E

A

A

V

I

S

R

A

R

V

I

T

S

S

N

binding adenosine-5'-diphosphate: F33 (= F34), D35 (= D36), E37 (= E38), R94 (= R94), R97 (≠ A97), H129 (= H127)
binding adenosine monophosphate: R97 (≠ A97), V99 (≠ F99), R100 (≠ H100), E131 (≠ H129), F145 (≠ Q146), S147 (≠ A148), V173 (≠ A179), A177 (= A184)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D218), D213 (= D219), M214 (≠ I220), D216 (≠ A222), T217 (= T223), G219 (≠ S225), T220 (= T226)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
29% identity, 97% coverage: 5:294/298 of query aligns to 4:297/307 of 7xmuA

query
sites
7xmuA

L

L

F

F

A

A

L

G

P

N

G

A

N

T

E

P

R

E

A

L

A

A

R

Q

A

R

L

I

A

A

S

N

A

R

L

L

G

Y

A

T

D

S

W

L

G

G

S

D

A

A

I

V

R

G

H

R

F
|
F

A

S

D
|
D

G

G

E
|
E

S

V

S

S

V

V

R

Q

L

I

L

N

S

E

P

N

L

V

Q

R

G

G

R

G

Q

D

A

I

I

F

L

I

F

I

C

Q

T

S

L

T

D

C

R

A

P

P

-

T

D

N

E

D

K

N

I

L

V

M

P

E

L

L

L

V

W

V

A

M

A

V

I

D

A

A

A

L

R

R

E

R

S

A

G

S

A

A

S

G

R

R

V

I

G

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

A
x
R

V

R

F

V

H
x
R

P

S

G

A

E

R

I

V

-

P

V

I

A

T

A

A

R

K

H

V

F

V

A

A

A

D

L

F

L

L

S

S

R

S

-

V

H

G

F

V

D

D

W

R

L

V

V

L

T

T

V

V

D

D

P

L

H
|
H

L

A

H
x
E

R

Q

I

I

A

Q

T

G

L

F

D

-

I

-

Y

F

T

D

I

V

P

P

T

V

Q

D

C

N

V

V

Q
x
F

A

G

A
x
S

P

P

A

I

I

L

-

L

A

E

S

D

W

M

L

L

R

Q

A

L

T

N

V

L

N

D

Q

N

P

P

F

I

L

V

V

V

G

S

P

P

D
|
D

E

-

S

-

R

-

Q

-

W

-

V

I

N

G

D

G

V

V

A
x
V

R

R

R

-

C

A

Q

R

A

A

P

I

A

A

I

K

V

L

L

L

H

N

K

D

T

T

R

D

H

M

A

A

-

I

D

D

R

K

C

R

V

R

E

Q

I

V

Q

M

A

H

A

I

D

-

L

I

N

G

A

D

A

V

A

A

G

G

C

R

T

D

P

C

V

V

L

L

L

V

D
|
D

D

D

I
x
M

V

I

A
x
D

T
|
T

G

G

S

G

T

T

M

L

V

C

A

K

A

A

N

E

L

A

L

L

R

K

R

E

A

R

G

G

L

A

A

K

P

R

P

V

V

F

C

A

V

Y

A

A

V

T

H

H

A

P

V

I

F

F

A

S

G

G

D

N

A

A

F

A

A

N

E

N

L

L

R

R

-

N

A

S

V

V

A

I

A

D

E

E

I

V

V

V

T

V

C

C

D

D

T

T

V

I

S

P

H

L

S

S

S

D

N

E

-

I

-

K

-

S

-

L

-

P

-

N

-

V

-

R

G

T

I

L

A

T

L

L

A

S

A

G

P

M

L

L

A

A

E

E

A

A

V

I

S

R

A

R

V

I

T

S

S

N

binding adenosine-5'-diphosphate: F33 (= F34), D35 (= D36), E37 (= E38), R94 (= R94), Q95 (= Q95), R97 (≠ A97), R97 (≠ A97), R100 (≠ H100), H129 (= H127), E131 (≠ H129), F145 (≠ Q146), S147 (≠ A148), V173 (≠ A179)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D168), D212 (= D218), M214 (≠ I220), D216 (≠ A222), T217 (= T223)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
27% identity, 97% coverage: 5:294/298 of query aligns to 5:304/308 of 4s2uA

query
sites
4s2uA

L

L

F

F

A

A

L

G

P

N

G

A

N

T

E

P

R

E

A

L

A

A

R

Q

A

R

L

I

A

A

S

N

A

R

L

L

G

Y

A

T

D

S

W

L

G

G

S

D

A

A

I

V

R

G

H

R

F

F

A

S

D

D

G

G

E

E

S

V

S

S

V

V

R

Q

L

I

L

N

S

E

P

N

L

V

Q

R

G

G

R

G

Q

D

A

I

I

F

L

I

F

I

C

Q

T

S

L

T

D

C

R

A

P

P

-

T

D

N

E

D

K

N

I

L

V

M

P

E

L

L

L

V

W

V

A

M

A

V

I

D

A

A

A

L

R

R

E

R

S

A

G

S

A

A

S

G

R

R

V

I

G

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

A

R

V

R

F

V

H

R

P

S

G

A

E

R

I

V

-

P

V

I

A

T

A

A

R

K

H

V

F

V

A

A

A

D

L

F

L

L

S

S

R

S

-

V

H

G

F

V

D

D

W

R

L

V

V

L

T

T

V

V

D

D

P

L

H

H

L

A

H

E

R

Q

I

I

A

Q

T

G

L

F

D

-

I

-

Y

F

T

D

I

V

P

P

T

V

Q

D

C

N

V

V

Q

F

A

G

A

S

P

P

A

I

I

L

-

L

A

E

S

D

W

M

L

L

R

Q

A

L

T

N

V

L

N

D

Q

N

P

P

F

I

L

V

V

V

G

S

P

P

D

D

E

I

E

G

S

G

R

V

Q

V

W

R

V

A

N

R

D

A

V

I

A

A

R

K

R

L

C

L

Q

N

A

D

P

T

A

D

I

M

V

A

L

I

H

I

K

D

T

K

R

R

H

R

A

P

D

R

R

A

C

N

V

V

E

S

I

Q

Q

V

A

M

A

H

D

I

L

I

N

G

A

D

A

V

A

A

G

G

C

R

T

D

P

C

V

V

L

L

L

V

D

D

I

M

V

I

A
x
D

T

T

G

G

S

G

T
|
T

M

L

V

C

A

K

A

A

N

E

L

A

L

L

R

K

R

E

A

R

G

G

L

A

A

K

P

R

P

V

V

F

C

A

V

Y

A

A

V

T

H

H

A

P

V

I

F

F

A

S

G

G

D

N

A

A

F

A

A

N

E

N

L

L

R

R

-

N

A

S

V

V

A

I

A

D

E

E

I

V

V

V

T

V

C

C

D

D

T

T

V

I

S

P

H

L

S

S

S

D

N

E

-

I

-

K

-

S

-

L

-

P

-

N

-

V

-

R

G

T

I

L

A

T

L

L

A

S

A

G

P

M

L

L

A

A

E

E

A

A

V

I

S

R

A

R

V

I

T

S

S

N

binding magnesium ion: D223 (≠ A222), T227 (= T226)

6asvC E. Coli prpp synthetase (see paper)
27% identity, 97% coverage: 5:294/298 of query aligns to 4:303/311 of 6asvC

query
sites
6asvC

L

L

F

F

A

A

L

G

P

N

G

A

N

T

E

P

R

E

A

L

A

A

R

Q

A

R

L

I

A

A

S

N

A

R

L

L

G

Y

A

T

D

S

W

L

G

G

S

D

A

A

I

V

R

G

H

R

F

F

A

S

D

D

G

G

E

E

S

V

S

S

V

V

R

Q

L

I

L

N

S

E

P

N

L

V

Q

R

G

G

R

G

Q

D

A

I

I

F

L

I

F

I

C

Q

T

S

L

T

D

C

R

A

P

P

-

T

D

N

E

D

K

N

I

L

V

M

P

E

L

L

L

V

W

V

A

M

A

V

I

D

A

A

A

L

R

R

E

R

S

A

G

S

A

A

S

G

R

R

V

I

G

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

A

R

V

R

F

V

H

R

P

S

G

A

E

R

I

V

-

P

V

I

A

T

A

A

R

K

H

V

F

V

A

A

A

D

L

F

L

L

S

S

R

S

-

V

H

G

F

V

D

D

W

R

L

V

V

L

T

T

V

V

D

D

P

L

H

H

L

A

H

E

R

Q

I

I

A

Q

T

G

L

F

D

-

I

-

Y

F

T

D

I

V

P

P

T

V

Q

D

C

N

V

V

Q

F

A

G

A

S

P

P

A

I

I

L

-

L

A

E

S

D

W

M

L

L

R

Q

A

L

T

N

V

L

N

D

Q

N

P

P

F

I

L

V

V

V

G

S

P

P

D
|
D

E

I

E

G

S

G

R

V

Q

V

W

R

V

A

N

R

D

A

V

I

A

A

R

K

R

L

C

L

Q

N

A

D

P

T

A

D

I

M

V

A

L

I

H

I

K

D

T

K

R

R

H

R

A

P

D

R

R

A

C

N

V

V

E

S

I

Q

Q

V

A

M

A

H

D

I

L

I

N

G

A

D

A

V

A

A

G

G

C

R

T

D

P

C

V

V

L

L

L

V

D
|
D

I

M

V

I

A
x
D

T
|
T

G
|
G

S

G

T
|
T

M
x
L

V

C

A

K

A

A

N

E

L

A

L

L

R

K

R

E

A

R

G

G

L

A

A

K

P

R

P

V

V

F

C

A

V

Y

A

A

V

T

H

H

A

P

V

I

F

F

A

S

G

G

D

N

A

A

F

A

A

N

E

N

L

L

R

R

-

N

A

S

V

V

A

I

A

D

E

E

I

V

V

V

T

V

C

C

D

D

T

T

V

I

S

P

H

L

S

S

S

D

N

E

-

I

-

K

-

S

-

L

-

P

-

N

-

V

-

R

G

T

I

L

A

T

L

L

A

S

A

G

P

M

L

L

A

A

E

E

A

A

V

I

S

R

A

R

V

I

T

S

S

N

binding phosphate ion: D168 (= D168), D218 (= D218), D219 (= D219), D222 (≠ A222), T223 (= T223), G224 (= G224), T226 (= T226), L227 (≠ M227)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
27% identity, 97% coverage: 5:294/298 of query aligns to 6:305/315 of P0A717

query
sites
P0A717

L

L

F

F

A

A

L

G

P

N

G

A

N

T

E

P

R

E

A

L

A

A

R

Q

A

R

L

I

A

A

S

N

A

R

L

L

G

Y

A

T

D

S

W

L

G

G

S

D

A

A

I

V

R

G

H

R

F

F

A

S

D

D

G

G

E

E

S

V

S

S

V

V

R

Q

L

I

L

N

S

E

P

N

L

V

Q

R

G

G

R

G

Q

D

A

I

I

F

L

I

F

I

C

Q

T

S

L

T

D

C

R

A

P

P

-

T

D

N

E

D

K

N

I

L

V

M

P

E

L

L

L

V

W

V

A

M

A

V

I

D

A

A

A

L

R

R

E

R

S

A

G

S

A

A

S

G

R

R

V

I

G

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

A

R

V

R

F

V

H

R

P

S

G

A

E

R

I

V

-

P

V

I

A

T

A

A

R

K

H

V

F

V

A

A

A

D

L

F

L

L

S

S

R

S

-

V

H

G

F

V

D

D

W

R

L

V

V

L

T

T

V

V

D
|
D

P

L

H

H

L

A

H

E

R

Q

I

I

A

Q

T

G

L

F

D

-

I

-

Y

F

T

D

I

V

P

P

T

V

Q

D

C

N

V

V

Q

F

A

G

A

S

P

P

A

I

I

L

-

L

A

E

S

D

W

M

L

L

R

Q

A

L

T

N

V

L

N

D

Q

N

P

P

F

I

L

V

V

V

G

S

P

P

D

D

E

I

E

G

S

G

R

V

Q

V

W

R

V

A

N

R

D

A

V

I

A

A

R

K

R

L

C

L

Q

N

A

D

P

T

A

D

I

M

V

A

L

I

H

I

K

D

T

K

R

R

H

R

A

P

D

R

R

A

C

N

V

V

E

S

I

Q

Q

V

A

M

A

H

D

I

L

I

N

G

A

D

A

V

A

A

G

G

C

R

T

D

P

C

V

V

L

L

L

V

D
|
D

I

M

V

I

A
x
D

T

T

G

G

S

G

T

T

M

L

V

C

A

K

A

A

N

E

L

A

L

L

R

K

R

E

A

R

G

G

L

A

A

K

P

R

P

V

V

F

C

A

V

Y

A

A

V

T

H

H

A

P

V

I

F

F

A

S

G

G

D

N

A

A

F

A

A

N

E

N

L

L

R

R

-

N

A

S

V

V

A

I

A

D

E

E

I

V

V

V

T

V

C

C

D

D

T

T

V

I

S

P

H

L

S

S

S

D

N

E

-

I

-

K

-

S

-

L

-

P

-

N

-

V

-

R

G

T

I

L

A

T

L

L

A

S

A

G

P

M

L

L

A

A

E

E

A

A

V

I

S

R

A

R

V

I

T

S

S

N

D129 (= D125) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D218) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D219) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (≠ A222) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q97CA5 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) (see paper)
24% identity, 84% coverage: 32:281/298 of query aligns to 30:273/286 of Q97CA5

query
sites
Q97CA5

R

R

H

R

F

F

A

P

D
|
D

G
|
G

E
|
E

S

L

S

Y

V

L

R

R

L

Y

L

D

S

E

P

D

L

L

Q

T

G

G

R

H

Q

N

A

I

I

F

L

I

F

I

C

G

T

N

L

T

D

-

R

H

P

S

D

D

E

A

K

E

I

V

V

M

P

E

L

M

L

I

W

L

A

T

A

L

I

S

A

A

A

I

R

Q

E

D

S

Y

G

R

A

T

S

K

R

S

V

V

G

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

A

Q

V

R

F

Y

H

K

P

N

G

G

E

E

I

P

V

I

A

S

R

Q

H

I

F

L

A

T

A

E

L

I

L

Y

S

S

R

S

H

Y

F

S

D

N

W

S

L

I

V

A

T

T

V

V

D

D

P

I

H

H

L

D

H

E

R

K

I

T

A

L

T

S

L

Y

D

S

D

-

I

-

Y

-

T

K

I

V

P

K

T

F

Q

S

C

D

V

L

Q

H

A

A

A

N

P

D

A

A

I

I

A

V

S

R

W

Y

L

Y

R

K

A

-

T

N

V

V

N

D

Q

V

P

D

F

Y

L

V

V

V

G

S

P

P

D

D

E

D

E

G

S

G

R

L

Q

A

W

R

V

V

N

A

D

D

V

I

A

S

R

A

R

K

C

L

Q

G

A

K

P

K

A

H

I

F

V

F

L

I

H

E

K

K

T

K

R
|
R

H

I

A

D

D

D

R

R

C

T

V

V

E

E

I

M

Q

K

A

V

A

P

D

N

L

V

N

D

A

V

A

N

A

-

G

G

C

K

T

K

P

L

V

L

L

I

L

V

D
|
D

D

D

I

I

V

I

A
x
S

T
|
T

G
|
G

S
x
G

T
|
T

M

I

V

A

A

K

A

S

N

G

L

L

R

R

R

E

A

K

G

G

L

A

A

S

P

K

P

I

V

Y

C

V

V

S

A

A

V

V

H

H

A

G

V

L

F

F

A

V

G

N

D

G

A

S

F

E

A

N

E

K

L

I

R

L

A

Q

V

N

A

A

A

D

E

E

I

I

V

H

T

V

C

T

D

D

T

T

V

V

S

E

H

S

S

K

S

F

N

S

G

D

I

I

A

S

L

V

DGE 34:36 (= DGE 36:38) binding
RQH 91:93 (≠ RQD 94:96) binding
R186 (= R193) binding
D210 (= D218) binding
STGGT 214:218 (≠ ATGST 222:226) binding

3mbiA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
24% identity, 84% coverage: 32:281/298 of query aligns to 32:275/287 of 3mbiA

query
sites
3mbiA

R

R

H

R

F
|
F

A

P

D
|
D

G

G

E
|
E

S

L

S

Y

V

L

R

R

L

Y

L

D

S

E

P

D

L

L

Q

T

G

G

R

H

Q

N

A

I

I

F

L

I

F

I

C

G

T

N

L

T

D

-

R

H

P

S

D

D

E

A

K

E

I

V

V

M

P

E

L

M

L

I

W

L

A

T

A

L

I

S

A

A

A

I

R

Q

E

D

S

Y

G

R

A

T

S

K

R

S

V

V

G

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

A

Q

V

R

F
x
Y

H

K

P

N

G

G

E

E

I

P

V

I

A

S

R

Q

H

I

F

L

A

T

A

E

L

I

L

Y

S

S

R

S

H

Y

F

S

D

N

W

S

L

I

V

A

T

T

V

V

D

D

P

I

H
|
H

L

D

H

E

R

K

I

T

A

L

T

S

L

Y

D

S

D

-

I

-

Y

-

T

K

I

V

P

K

T

F

Q

S

C

D

V

L

Q

H

A

A

A

N

P

D

A

A

I

I

A

V

S

R

W

Y

L

Y

R

K

A

-

T

N

V

V

N

D

Q

V

P

D

F

Y

L

V

V

V

G

S

P

P

D

D

E

D

E

G

S

G

R

L

Q

A

W

R

V

V

N

A

D

D

V

I

A

S

R

A

R

K

C

L

Q

G

A

K

P

K

A

H

I

F

V

F

L

I

H

E

K
|
K

T

K

R
|
R

H

I

A

D

D

D

R

R

C

T

V

V

E

E

I

M

Q

K

A

V

A

P

D

N

L

V

N

D

A

V

A

N

A

-

G

G

C

K

T

K

P

L

V

L

L

I

L

V

D

D

I

I

V

I

A
x
S

T
|
T

G
|
G

S

G

T
|
T

M

I

V

A

A

K

A

S

N

G

L

L

R

R

R

E

A

K

G

G

L

A

A

S

P

K

P

I

V

Y

C

V

V

S

A

A

V

V

H

H

A

G

V

L

F

F

A

V

G

N

D

G

A

S

F

E

A

N

E

K

L

I

R

L

A

Q

V

N

A

A

A

D

E

E

I

I

V

H

T

V

C

T

D

D

T

T

V

V

S

E

H

S

S

K

S

F

N

S

G

D

I

I

A

S

L

V

binding adenosine-5'-diphosphate: F34 (= F34), D36 (= D36), E38 (= E38), R93 (= R94), Q94 (= Q95), Y98 (≠ F99), H126 (= H127), K186 (= K191), R188 (= R193)
binding magnesium ion: H95 (≠ D96), H126 (= H127)
binding phosphate ion: R188 (= R193), S216 (≠ A222), T217 (= T223), G218 (= G224), T220 (= T226)

3mbiD Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
24% identity, 84% coverage: 32:281/298 of query aligns to 30:273/285 of 3mbiD

query
sites
3mbiD

R

R

H

R

F
|
F

A

P

D
|
D

G

G

E
|
E

S

L

S

Y

V

L

R

R

L

Y

L

D

S

E

P

D

L

L

Q

T

G

G

R

H

Q

N

A

I

I

F

L

I

F

I

C

G

T

N

L

T

D

-

R

H

P

S

D

D

E

A

K

E

I

V

V

M

P

E

L

M

L

I

W

L

A

T

A

L

I

S

A

A

A

I

R

Q

E

D

S

Y

G

R

A

T

S

K

R

S

V

V

G

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

H

A

Q

V

R

F
x
Y

H

K

P

N

G

G

E

E

I

P

V

I

A

S

R

Q

H

I

F

L

A

T

A

E

L

I

L

Y

S

S

R

S

H

Y

F

S

D

N

W

S

L

I

V

A

T

T

V

V

D

D

P

I

H
|
H

L

D

H

E

R

K

I

T

A

L

T

S

L

Y

D

S

D

-

I

-

Y

-

T

K

I

V

P

K

T

F

Q

S

C

D

V

L

Q

H

A

A

A

N

P

D

A

A

I

I

A

V

S

R

W

Y

L

Y

R

K

A

-

T

N

V

V

N

D

Q

V

P

D

F

Y

L

V

V

V

G

S

P

P

D

D

E

D

E

G

S

G

R

L

Q

A

W

R

V

V

N

A

D

D

V

I

A

S

R

A

R

K

C

L

Q

G

A

K

P

K

A

H

I

F

V

F

L

I

H

E

K
|
K

T

K

R
|
R

H

I

A

D

D

D

R

R

C

T

V

V

E

E

I

M

Q

K

A

V

A

P

D

N

L

V

N

D

A

V

A

N

A

-

G

G

C

K

T

K

P

L

V

L

L

I

L

V

D
|
D

I
|
I

V

I

A
x
S

T
|
T

G
|
G

S

G

T
|
T

M

I

V

A

A

K

A

S

N

G

L

L

R

R

R

E

A

K

G

G

L

A

A

S

P

K

P

I

V

Y

C

V

V

S

A

A

V

V

H

H

A

G

V

L

F

F

A

V

G

N

D

G

A

S

F

E

A

N

E

K

L

I

R

L

A

Q

V

N

A

A

A

D

E

E

I

I

V

H

T

V

C

T

D

D

T

T

V

V

S

E

H

S

S

K

S

F

N

S

G

D

I

I

A

S

L

V

binding adenosine-5'-diphosphate: F32 (= F34), D34 (= D36), E36 (= E38), R91 (= R94), Q92 (= Q95), Y96 (≠ F99), H124 (= H127), K184 (= K191), R186 (= R193)
binding 5-O-phosphono-alpha-D-ribofuranose: R91 (= R94), H124 (= H127), K184 (= K191), R186 (= R193), D210 (= D218), D211 (= D219), I212 (= I220), S214 (≠ A222), T215 (= T223), G216 (= G224), T218 (= T226)

3lpnA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with an atp analog (ampcpp). (see paper)
24% identity, 84% coverage: 32:281/298 of query aligns to 30:273/284 of 3lpnA

query
sites
3lpnA

R

R

H

R

F
|
F

A

P

D
|
D

G

G

E
|
E

S

L

S

Y

V

L

R

R

L

Y

L

D

S

E

P

D

L

L

Q

T

G

G

R

H

Q

N

A

I

I

F

L

I

F

I

C

G

T

N

L

T

D

-

R

H

P
x
S

D

D

E

A

K

E

I

V

V

M

P

E

L

M

L

I

W

L

A

T

A

L

I

S

A

A

A

I

R

Q

E

D

S

Y

G

R

A

T

S

K

R

S

V

V

G

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

A

Q

V

R

F
x
Y

H

K

P

N

G

G

E

E

I

P

V

I

A

S

R

Q

H

I

F

L

A

T

A

E

L

I

L

Y

S

S

R

S

H

Y

F

S

D

N

W

S

L

I

V

A

T

T

V

V

D

D

P

I

H
|
H

L
x
D

H

E

R

K

I

T

A

L

T

S

L

Y

D

S

D

-

I

-

Y

-

T

K

I

V

P

K

T

F

Q

S

C

D

V

L

Q

H

A

A

A

N

P

D

A

A

I

I

A

V

S

R

W

Y

L

Y

R

K

A

-

T

N

V

V

N

D

Q

V

P

D

F

Y

L

V

V

V

G

S

P

P

D

D

E

D

E

G

S

G

R

L

Q

A

W

R

V

V

N

A

D

D

V

I

A

S

R

A

R

K

C

L

Q

G

A

K

P

K

A

H

I

F

V

F

L

I

H

E

K

K

T

K

R

R

H

I

A

D

D

D

R

R

C

T

V

V

E

E

I

M

Q

K

A

V

A

P

D

N

L

V

N

D

A

V

A

N

A

-

G

G

C

K

T

K

P

L

V

L

L

I

L

V

D

D

I

I

V

I

A
x
S

T
|
T

G
|
G

S

G

T
|
T

M

I

V

A

A

K

A

S

N

G

L

L

R

R

R

E

A

K

G

G

L

A

A

S

P

K

P

I

V

Y

C

V

V

S

A

A

V

V

H

H

A

G

V

L

F

F

A

V

G

N

D

G

A

S

F

E

A

N

E

K

L

I

R

L

A

Q

V

N

A

A

A

D

E

E

I

I

V

H

T

V

C

T

D

D

T

T

V

V

S

E

H

S

S

K

S

F

N

S

G

D

I

I

A

S

L

V

binding diphosphomethylphosphonic acid adenosyl ester: F32 (= F34), D34 (= D36), E36 (= E38), R91 (= R94), Q92 (= Q95), H93 (≠ D96), Y96 (≠ F99), H124 (= H127), D125 (≠ L128)
binding sulfate ion: S58 (≠ P61), S214 (≠ A222), T215 (= T223), G216 (= G224), T218 (= T226)

Sites not aligning to the query:

binding sulfate ion: 7, 11, 15

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
25% identity, 90% coverage: 5:273/298 of query aligns to 12:280/317 of P14193

query
sites
P14193

L

I

F

F

A

S

L

L

P

N

G

S

N

N

E

P

R

E

A

L

A

A

R

K

A

E

L

I

A

A

S

D

A

I

L

V

G

G

A

V

D

Q

W

L

G

G

S

K

A

C

A

S

I

V

R

T

H

R

F

F

A

S

D

D

G

G

E

E

S

V

S

Q

V

I

R

N

L

I

L

E

S

E

P

S

L

I

Q

R

G

G

R

C

Q

D

A

C

-

Y

I

I

L

I

F

Q

C

S

T

T

L

S

D

D

R

P

P

V

D

N

E

E

K

H

I

I

V

M

P

E

L

L

W

I

A

M

A

V

I

D

A

A

A

L

R

K

E

R

S

A

G

S

A

A

S

K

R

T

V

I

G

N

L

I

V

V

A

I

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

A

R

V

K

F

A

H

R

P

S

G

R

E

E

I

P

V

I

A

T

A

A

R

K

H

L

F

F

A

A

A

N

L

L

S

E

R

T

-

A

H

G

F

A

D

T

W

R

L

V

V

I

T

A

V

L

D

D

P

L

H

H

L

A

H

P

R

Q

I

I

A

Q

T

G

L

F

D

-

I

-

Y

F

T

D

I

I

P

P

T

I

Q

D

C

H

V

L

Q

M

A

G

A

V

P

P

A

I

I

L

A

G

S

E

W

Y

L

F

R

E

A

G

-

K

T

N

V

L

N

E

Q

D

P

I

F

V

L

I

V

V

G

S

P

P

D

D

E

H

E

G

S

G

R

V

Q

T

W

R

V

A

N

R

D

K

V

L

A

A

R

D

R

R

C

L

Q

K

A

A

P

P

A

I

I

A

V

I

L

I

H

D

K
|
K

T

R

R
|
R

H

P

A
x
R

D

P

R
x
N

C

V

V

A

E
|
E

I

V

Q

M

A

N

A

I

D

-

L

V

N

G

A

N

A

I

A

E

G

G

C

K

T

T

P

A

V

I

L

L

L

I

D

D

I

I

V

I

A
x
D

T
|
T

G
x
A

S
x
G

T
|
T

M

I

V

T

A

L

A

A

N

N

L

A

L

L

R

V

R

E

A

N

G

G

L

A

A

K

P

E

P

V

V

Y

C

A

V

C

A

C

V

T

H

H

A

P

V

V

F

L

A

S

G

G

D

P

A

A

F

V

A

E

E

R

L

I

-

N

R

N

A

S

V

T

A

I

A

K

E

E

I

L

V

V

T

V

C

T

D

N

T

S

V

I

S

K

RQ 102:103 (= RQ 94:95) binding
K198 (= K191) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R193) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ A195) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (≠ R197) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (= E200) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (≠ ATGST 222:226) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
25% identity, 90% coverage: 5:273/298 of query aligns to 4:263/295 of 1dkuA

query
sites
1dkuA

L

I

F

F

A

S

L

L

P

N

G

S

N

N

E

P

R

E

A

L

A

A

R

K

A

E

L

I

A

A

S

D

A

I

L

V

G

G

A

V

D

Q

W

L

G

G

S

K

A

C

A

S

I

V

R

T

H

R

F

F

A

S

D

D

G

G

E

E

S

V

S

Q

V

I

R

N

L

I

L

E

S

E

P

S

L

I

Q

R

G

G

R

C

Q

D

A

C

-

Y

I

I

L

I

F

Q

C

S

T

T

L

S

D

D

R

P

P

V

D

N

E

E

K

H

I

I

V

M

P

E

L

L

W

I

A

M

A

V

I

D

A

A

A

L

R

K

E

R

S

A

G

S

A

A

S

K

R

T

V

I

G

N

L

I

V

V

A

I

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q

Q

D

D

A

R

V
x
K

F

A

H

R

P
x
S

G
x
R

E

E

I

P

V

I

A

T

A

A

R

K

H

L

F

F

A

A

A

N

L

L

S

E

R

T

-

A

H

G

F

A

D

T

W

R

L

V

V

I

T

A

V

L

D

D

P

L

H
|
H

L

A

H

P

R

Q

I

I

A

Q

T

G

L

F

D

-

I

-

Y

F

T

D

I

I

P

P

T

I

Q
x
D

C

H

V

L

Q

M

A

G

A

V

P

P

A

I

I

L

A

G

S

E

W

Y

L

F

R

E

A

G

-

K

T

N

V

L

N

E

Q

D

P

I

F

V

L

I

V

V

G

S

P

P

D

D

E

H

E

G

S

G

R

V

Q

T

W

R

V

A

N

R

D

K

V

L

A

A

R

D

R

R

C

L

Q

K

A

A

P

P

A

I

I

A

V

I

L

I

H

D

K

K

T

R

R

M

H

N

A

I

D

V

R

G

C

N

V

I

E

E

I

-

Q

-

A

-

D

-

L

-

N

-

A

-

G

G

C

K

T

T

P

A

V

I

L

L

L

I

D

D

I

I

V

I

A

D

T

T

G

A

S

G

T

T

M

I

V

T

A

L

A

A

N

N

L

A

L

L

R

V

R

E

A

N

G

G

L

A

A

K

P

E

P

V

V

Y

C

A

V

C

A

C

V

T

H

H

A

P

V

V

F

L

A

S

G

G

D

P

A

A

F

V

A

E

E

R

L

I

-

N

R

N

A

S

V

T

A

I

A

K

E

E

I

L

V

V

T

V

C

T

D

N

T

S

V

I

S

K

binding methyl phosphonic acid adenosine ester: R94 (= R94), H128 (= H127)
binding phosphomethylphosphonic acid adenosyl ester: K98 (≠ V98), S101 (≠ P101), R102 (≠ G102), D141 (≠ Q143)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenosyl ester: 290, 291, 292, 295

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
25% identity, 90% coverage: 5:273/298 of query aligns to 6:263/299 of 1ibsB

query
sites
1ibsB

L

I

F

F

A

S

L

L

P

N

G

S

N

N

E

P

R

E

A

L

A

A

R

K

A

E

L

I

A

A

S

D

A

I

L

V

G

G

A

V

D

Q

W

L

G

G

S

K

A

C

A

S

I

V

R

T

H

R

F

F

A

S

D

D

G

G

E

E

S

V

S

Q

V

I

R

N

L

I

L

E

S

E

P

S

L

I

Q

R

G

G

R

C

Q

D

A

C

-

Y

I

I

L

I

F

Q

C

S

T

T

L

S

D

D

R

P

P

V

D

N

E

E

K

H

I

I

V

M

P

E

L

L

W

I

A

M

A

V

I

D

A

A

A

L

R

K

E

R

S

A

G

S

A

A

S

K

R

T

V

I

G

N

L

I

V

V

A

I

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

A
x
R

V

-

F

-

H

K

P

S

G

R

E

E

I

P

V

I

A

T

A

A

R

K

H

L

F

F

A

A

A

N

L

L

S

E

R

T

-

A

H

G

F

A

D

T

W

R

L

V

V

I

T

A

V

L

D

D

P

L

H

H

L

A

H

P

R

Q

I

I

A

Q

T

G

L

F

D

-

I

-

Y

F

T

D

I

I

P

P

T

I

Q

D

C

H

V

L

Q

M

A

G

A

V

P

P

A

I

I

L

A

G

S

E

W

Y

L

F

R

E

A

G

-

K

T

N

V

L

N

E

Q

D

P

I

F

V

L

I

V

V

G

S

P

P

D

D

E

H

E

G

S

G

R

V

Q

T

W

R

V

A

N

R

D

K

V

L

A

A

R

D

R

R

C

L

Q

K

A

A

P

P

A

I

I

A

V

I

L

I

H

D

K

K

T

R

R

M

H

N

A

I

D

V

R

G

C

N

V

I

E

E

I

-

Q

-

A

-

D

-

L

-

N

-

A

-

G

G

C

K

T

T

P

A

V

I

L

L

L

I

D

D

I

I

V

I

A

D

T

T

G

A

S

G

T

T

M

I

V

T

A

L

A

A

N

N

L

A

L

L

R

V

R

E

A

N

G

G

L

A

A

K

P

E

P

V

V

Y

C

A

V

C

A

C

V

T

H

H

A

P

V

V

F

L

A

S

G

G

D

P

A

A

F

V

A

E

E

R

L

I

-

N

R

N

A

S

V

T

A

I

A

K

E

E

I

L

V

V

T

V

C

T

D

N

T

S

V

I

S

K

binding methyl phosphonic acid adenosine ester: R96 (= R94), Q97 (= Q95), R99 (≠ A97)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
25% identity, 90% coverage: 5:273/298 of query aligns to 4:261/297 of 1ibsA

query
sites
1ibsA

L

I

F

F

A

S

L

L

P

N

G

S

N

N

E

P

R

E

A

L

A

A

R

K

A

E

L

I

A

A

S

D

A

I

L

V

G

G

A

V

D

Q

W

L

G

G

S

K

A

C

A

S

I

V

R

T

H

R

F

F

A

S

D

D

G

G

E

E

S

V

S

Q

V

I

R

N

L

I

L

E

S

E

P

S

L

I

Q

R

G

G

R

C

Q

D

A

C

-

Y

I

I

L

I

F

Q

C

S

T

T

L

S

D

D

R

P

P

V

D

N

E

E

K

H

I

I

V

M

P

E

L

L

W

I

A

M

A

V

I

D

A

A

A

L

R

K

E

R

S

A

G

S

A

A

S

K

R

T

V

I

G

N

L

I

V

V

A

I

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

A
x
R

V

-

F

-

H

K

P

S

G

R

E

E

I

P

V

I

A

T

A

A

R

K

H

L

F

F

A

A

A

N

L

L

S

E

R

T

-

A

H

G

F

A

D

T

W

R

L

V

V

I

T

A

V

L

D

D

P

L

H
|
H

L

A

H

P

R

Q

I

I

A

Q

T

G

L

F

D

-

I

-

Y

F

T

D

I

I

P

P

T

I

Q

D

C

H

V

L

Q

M

A

G

A

V

P

P

A

I

I

L

A

G

S

E

W

Y

L

F

R

E

A

G

-

K

T

N

V

L

N

E

Q

D

P

I

F

V

L

I

V

V

G

S

P

P

D

D

E

H

E

G

S

G

R

V

Q

T

W

R

V

A

N

R

D

K

V

L

A

A

R

D

R

R

C

L

Q

K

A

A

P

P

A

I

I

A

V

I

L

I

H

D

K

K

T

R

R

M

H

N

A

I

D

V

R

G

C

N

V

I

E

E

I

-

Q

-

A

-

D

-

L

-

N

-

A

-

G

G

C

K

T

T

P

A

V

I

L

L

L

I

D

D

I

I

V

I

A
x
D

T
|
T

G
x
A

S

G

T
|
T

M

I

V

T

A

L

A

A

N

N

L

A

L

L

R

V

R

E

A

N

G

G

L

A

A

K

P

E

P

V

V

Y

C

A

V

C

A

C

V

T

H

H

A

P

V

V

F

L

A

S

G

G

D

P

A

A

F

V

A

E

E

R

L

I

-

N

R

N

A

S

V

T

A

I

A

K

E

E

I

L

V

V

T

V

C

T

D

N

T

S

V

I

S

K

binding methyl phosphonic acid adenosine ester: R94 (= R94), Q95 (= Q95), R97 (≠ A97), H126 (= H127)
binding sulfate ion: D209 (≠ A222), T210 (= T223), A211 (≠ G224), T213 (= T226)

7pn0A Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus at r32 space group
27% identity, 90% coverage: 5:271/298 of query aligns to 5:272/312 of 7pn0A

query
sites
7pn0A

L

I

F

F

A

S

L

G

P

Q

G

S

N

N

E

R

R

P

A

L

A

A

R

Q

A

A

L

I

A

A

S

E

A

A

L

L

G

G

A

L

D

P

W

L

G

G

S

K

A

S

A

T

I

T

R

L

H

R

F

F

A

A

D

N

G

D

E

N

S

L

S

F

V

V

R

R

L

Y

L

E

S

E

P

S

L

L

Q

R

G

E

R

G

Q

D

A

V

I

F

L

I

F

V

C

Q

T

S

L

F

D

V

R

P

P

P

-

V

D

Q

E

D

K

H

I

L

V

M

P

E

L

L

W

M

A

M

A

V

I

D

A

A

R

K

E

G

S

A

G

S

A

A

S

A

R

R

V

V

G

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

S

Y

Y

M

A

R
|
R

Q
x
S

D

D

A

K

V

K

F

D

H

A

P

P

G

R

E

I

I

S

V

I

A

T

A

A

R

R

H

L

F

I

A

A

A

D

L

L

-

Q

S

T

R

A

H

G

F

A

D

D

W

R

L

V

V

L

T

T

V

M

D

T

P

L

H
|
H

L

S

H

P

R

Q

I

V

A

-

T

-

L

-

D

H

D

G

I

F

Y

F

T

K

I

I

P

P

T

V

Q

D

C

H

V

L

Q

S

A

A

A

E

P

P

A

V

I

I

A

A

S

N

W

Y

L

F

-

A

-

T

R

R

A

V

T

D

V

L

N

E

Q

N

P

A

F

V

L

V

V

V

G

A

P

P

D

D

E

A

E

G

S

D

R

L

Q

K

W

R

V

A

N

S

D

A

V

L

A

A

R

R

C

L

Q

G

A

L

P

P

A

L

I

A

V

F

L

I

H

D

K

K

T

E

R

R

H

V

A

S

D

D

R

T

C

E

V

V

E

R

I

V

Q

R

A

M

A

L

D

-

L

V

N

G

A

E

A

V

A

E

G

G

C

K

T

T

P

A

V

L

L

I

L

V

D

D

I

E

V

I

A

S

T

T

G

A

S

G

T

S

M

L

V

V

A

E

A

A

A

V

N

E

L

A

L

L

R

M

R

Q

A

A

G

G

L

A

A

K

P

E

P

V

V

Y

C

A

V

A

A

A

V

T

H

H

A

G

V

V

F

Y

A

V

G

G

D

P

A

A

F

L

A

D

E

R

L

I

-

A

R

K

A

S

V

P

A

V

A

K

E

E

I

V

V

A

T

A

C

T

D

D

T

T

binding adenosine-5'-diphosphate: R95 (= R94), S96 (≠ Q95), H129 (= H127)

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
25% identity, 96% coverage: 5:289/298 of query aligns to 4:292/305 of 2hcrA

query
sites
2hcrA

L

I

F

F

A

S

L

G

P

S

G

S

N

H

E

Q

R

D

A

L

A

S

R

Q

A

K

L

I

A

A

S

D

A

R

L

L

G

G

A

L

D

E

W

L

G

G

S

K

A

V

I

T

R

K

H

K

F

F

A

S

D

N

G

Q

E

E

S

T

S

C

V

V

R

E

L

I

L

G

S

E

P

S

L

V

Q

R

G

G

R

E

Q

D

A

V

-

Y

I

I

L

V

F

Q

C

S

T

G

L

C

D

G

R

E

P

I

D

N

E

D

K

N

I

L

V

M

P

E

L

L

W

I

A

M

A

I

I

N

A

A

A

C

R

K

E

I

S

A

G

S

A

A

S

S

R

R

V

V

G

T

L

A

V

V

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

A
x
K

V

K

F

D

H

K

P

S

G

R

E

A

I

P

V

I

A

S

A

A

R

K

H

L

F

V

A

A

A

N

L

M

L

L

S

S

-

V

R

A

H

G

F

A

D

D

W

H

L

I

V

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

I

I

A

Q

T

G

L

F

D

-

I

-

Y

F

T

D

I

I

P

P

T

V

Q

D

C

N

V

L

Q

Y

A

A

A

E

P

P

A

A

I

V

A

L

S

K

W

W

L

I

R

R

A

E

T

N

V

I

N

S

Q

E

-

W

-

R

-

N

P

C

F

T

L

I

V

V

G

S

P

P

D

D

E

A

E

G

S

G

R

A

Q

K

W

R

V

V

N

T

D

S

V

I

A

A

R

D

R

R

C

L

Q

N

A

V

P

D

A

F

I

A

V

L

L

I

H

H

K

K

T

E

R

R

H

-

A

-

D

D

R

R

C

M

V

V

E

L

I

V

Q

G

A

D

A

V

D

K

L

D

N

R

A

V

A

A

A

-

G

-

C

-

T

-

P

-

V

I

L

L

L

V

D

D

I

M

V

A

A

D

T

T

G

C

S

G

T

T

M

I

V

C

A

H

A

A

N

D

L

K

L

L

R

L

R

S

A

A

G

G

L

A

A

T

P

R

P

V

V

Y

C

A

V

I

A

L

V

T

H

H

A

G

V

I

F

F

A

S

G

G

D

P

A

A

F

I

A

S

E

R

L

I

R

N

A

N

V

A

A

C

A

F

E

E

I

A

V

V

T

V

C

V

-

T

-

N

-

T

-

I

-

P

-

Q

-

E

D

D

T

K

V

M

S

K

H

H

S

C

S

S

N

K

-

I

-

Q

G

V

I

I

A

D

L

I

A

S

A

M

P

I

L

L

A

A

E

E

A

A

V

I

binding adenosine monophosphate: R94 (= R94), Q95 (= Q95), K97 (≠ A97), H128 (= H127)

5t3oA Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus (see paper)
27% identity, 90% coverage: 5:271/298 of query aligns to 4:271/307 of 5t3oA

query
sites
5t3oA

L

I

F

F

A

S

L

G

P

Q

G

S

N

N

E

R

R

P

A

L

A

A

R

Q

A

A

L

I

A

A

S

E

A

A

L

L

G

G

A

L

D

P

W

L

G

G

S

K

A

S

A

T

I

T

R

L

H

R

F

F

A

A

D

N

G

D

E

N

S

L

S

F

V

V

R

R

L

Y

L

E

S

E

P
x
S

L

L

Q
x
R

G

E

R

G

Q

D

A

V

I

F

L

I

F

V

C

Q

T

S

L

F

D

V

R

P

P

P

-

V

D

Q

E

D

K

H

I

L

V

M

P

E

L

L

W

M

A

M

A

V

I

D

A

A

R

K

E

G

S

A

G

S

A

A

S

A

R

R

V

V

G

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

S

Y

Y

M

A

R
|
R

Q
x
S

D

D

A

K

V

K

F
x
D

H

A

P

P

G

R

E

I

I

S

V

I

A

T

A

A

R

R

H

L

F

I

A

A

A

D

L

L

-

Q

S

T

R

A

H

G

F

A

D

D

W

R

L

V

V

L

T

T

V

M

D

T

P

L

H
|
H

L

S

H

P

R

Q

I

V

A

-

T

-

L

-

D

H

D

G

I

F

Y

F

T

K

I

I

P

P

T

V

Q

D

C

H

V

L

Q

S

A

A

A

E

P

P

A

V

I

I

A

A

S

N

W

Y

L

F

-

A

-

T

R

R

A

V

T

D

V

L

N

E

Q

N

P

A

F

V

L

V

V

V

G

A

P

P

D

D

E

A

E

G

S

D

R

L

Q

K

W

R

V

A

N

S

D

A

V

L

A

A

R

R

C

L

Q

G

A

L

P

P

A

L

I

A

V

F

L

I

H

D

K

K

T

E

R
|
R

H

V

A

S

D

D

R

T

C

E

V

V

E

R

I

V

Q

R

A

M

A

L

D

-

L

V

N

G

A

E

A

V

A

E

G

G

C

K

T

T

P

A

V

L

L

I

L

V

D

D

I

E

V

I

A

S

T

T

G

A

S

G

T

S

M

L

V

V

A

E

A

A

A

V

N

E

L

A

L

L

R

M

R

Q

A

A

G

G

L

A

A

K

P

E

P

V

V

Y

C

A

V

A

A

A

V

T

H

H

A

G

V

V

F

Y

A

V

G

G

D

P

A

A

F

L

A

D

E

R

L

I

-

A

R

K

A

S

V

P

A

V

A

K

E

E

I

V

V

A

T

A

C

T

D

D

T

T

binding adenosine-5'-diphosphate: R94 (= R94), S95 (≠ Q95), D99 (≠ F99), H128 (= H127), R193 (= R193)
binding sulfate ion: S45 (≠ P46), R47 (≠ Q48)

Query Sequence

>RR42_RS11675 FitnessBrowser__Cup4G11:RR42_RS11675
MKPTLFALPGNERAARALASALGADWGSAAIRHFADGESSVRLLSPLQGRQAILFCTLDR
PDEKIVPLLWAAIAARESGASRVGLVAPYLAYMRQDAVFHPGEIVAARHFAALLSRHFDW
LVTVDPHLHRIATLDDIYTIPTQCVQAAPAIASWLRATVNQPFLVGPDEESRQWVNDVAR
RCQAPAIVLHKTRHADRCVEIQAADLNAAAGCTPVLLDDIVATGSTMVAAANLLRRAGLA
PPVCVAVHAVFAGDAFAELRAVAAEIVTCDTVSHSSNGIALAAPLAEAVSAVTSIASR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory