SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS12995 FitnessBrowser__Cup4G11:RR42_RS12995 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A1P6 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
65% identity, 99% coverage: 4:471/471 of query aligns to 2:469/469 of P0A1P6

query
sites
P0A1P6

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E130 (= E132) binding Mn(2+)
E132 (= E134) binding Mn(2+)
E208 (= E210) binding ATP
E213 (= E215) binding Mn(2+)
E221 (= E223) binding Mn(2+)
NG 265:266 (= NG 267:268) binding L-glutamate
H270 (= H272) binding Mn(2+)
HMS 272:274 (≠ HQS 274:276) binding ATP
R322 (= R324) binding L-glutamate
E358 (= E360) binding Mn(2+)
R360 (= R362) binding L-glutamate

P0A9C5 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Escherichia coli (strain K12) (see 3 papers)
65% identity, 99% coverage: 4:471/471 of query aligns to 2:469/469 of P0A9C5

query
sites
P0A9C5

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Y398 (= Y400) modified: O-AMP-tyrosine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1fpyA Crystal structure of glutamine synthetase from salmonella typhimurium with inhibitor phosphinothricin (see paper)
65% identity, 99% coverage: 4:471/471 of query aligns to 1:468/468 of 1fpyA

query
sites
1fpyA

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T

T

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

F

Y

E
|
E

A

A

P

P

V

V

K

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

A

A

N

S

P

P

K

K

G

A

R

R

R
|
R

I

I

E
|
E

T

V

R
|
R

F

F

P

P

D

D

P

P

L

A

M

A

N

N

P

P

Y

Y

L

L

G

C

F

F

S

A

A

A

L

L

L

L

M

M

A

A

G

G

L

L

D

D

G

G

V

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

E

E

A

P

A

M

D

D

K

K

N

N

L

L

Y

Y

D

D

L

L

P

P

P

P

E

E

E

E

D

A

A

K

K

E

I

I

P

P

T

Q

V

V

C

A

S

G

S

S

L

L

D

E

Q

E

A

A

L

L

E

N

Y

A

L

L

D

D

N

L

D

D

R

R

E

E

F

F

L

L

T

K

R

A

G

G

G

G

V

V

F

F

S

T

N

D

S

E

M

A

I

I

D

D

A

A

Y

Y

I

I

E

A

L

L

K

R

M

R

E

E

E

E

V

D

T

D

R

R

F

V

R

R

M

M

T

T

T

P

H

H

P

P

I

V

E

E

F

F

D

E

M

L

Y

Y

Y

Y

S

S

L

V

binding adenosine-5'-diphosphate: G127 (= G130), E129 (= E132), E207 (= E210), T223 (= T226), F225 (= F228), H271 (= H274), S273 (= S276), R355 (= R358), E357 (= E360)
binding manganese (ii) ion: E129 (= E132), E131 (= E134), E212 (= E215), E220 (= E223), H269 (= H272), E357 (= E360)
binding phosphinothricin: E131 (= E134), E212 (= E215), G265 (= G268), H269 (= H272), R321 (= R324), E327 (= E330), R359 (= R362)

1f1hA Crystal structure of glutamine synthetase from salmonella typhimurium with thallium ions (see paper)
65% identity, 99% coverage: 4:471/471 of query aligns to 1:468/468 of 1f1hA

query
sites
1f1hA

S

S

V

A

A

E

D

H

V

V

M

L

K

T

L

M

V

L

K

N

E

E

N

H

D

E

V

V

K

K

F

F

V

V

D

D

F

L

R

R

F

F

T

T

D

D

T

T

K

K

G

G

K

K

E

E

Q

Q

H

H

V

V

S

T

V

I

P

P

V

A

S

H

H

Q

F

V

D

N

E

A

D

E

K

F

F

F

E

E

S

E

G

G

H

K

A

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

K

G

G

I

I

E

N

A

E

S

S

D

D

M

M

L

V

L

L

M

M

P

P

D

D

P

A

N

S

T

T

A

A

N

V

V

I

D

D

P

P

F

F

Y

F

E

A

E

D

P

S

T

T

L

L

I

I

L

I

T

R

C

C

D

D

V

I

V

L

E

E

P

P

S

G

D

T

G

L

K

Q

G

G

Y

Y

D

D

R

R

D

D

P

P

R

R

S

S

I

I

A

A

K

K

R

R

A

A

E

E

A

D

Y

Y

L

L

K

R

S

A

T

T

G

G

L

I

G

A

D

D

T

T

A

V

F

L

F

F

G

G

P

P

E
|
E

P

P

E
|
E

F

F

F

F

I

L

F

F

D

D

G

D

V

I

T

R

W

F

N

G

I

A

D

S

M

I

Q

S

G

G

C

S

S

H

V

V

K

A

I

I

H

D

S

D

E

I

E

E

A

G

P

A

W

W

S

N

S

S

G

S

K

T

E

K

F

Y

E

E

H

G

G

G

N

N

S

K

G

G

H

H

R

R

P

P

G

G

K

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

A

P

P

P

I

V

D

D

T

S

F

A

Q

Q

D

D

I

I

R

R

S

S

E

E

M

M

C

C

L

L

I

V

L

M

E

E

S

Q

L

M

G

G

I

L

P

V

V

V

E
|
E

V

A

H

H

H
|
H

H

H

E
|
E

V

V

A

A

G

T

Q

A

G

G

Q

Q

N

N

E
|
E

I

V

G

A

T
|
T

K

R

F
|
F

S

N

T

T

L

M

V

T

Q

K

R

K

A

A

D

D

W

E

T

I

Q

Q

L

I

Q

Y

K

K

Y

Y

V

V

I

V

Q

H

N

N

V

V

A

A

H

H

T

R

Y

F

G

G

K

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

M

V

F

G

G

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

C

H
|
H

Q

M

S
|
S

V

L

W

A

K

K

D

N

G

G

Q

T

N

N

L

L

F

F

A

S

G

G

N

D

G

K

Y

Y

A

A

G

G

L

L

S

S

E

E

F

Q

A

A

L

L

Y

Y

Y

Y

I

I

G

G

G

G

I

V

I

I

K

K

H

H

A

A

R

K

A

A

L

I

N

N

A

A

L

L

T

A

N

N

P

P

G

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

Y

E

E

A

A

P

P

V

V

K

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

A

A

N

S

P

P

K

K

G

A

R

R

R
|
R

I

I

E
|
E

T

V

R

R

F

F

P

P

D

D

P

P

L

A

M

A

N

N

P

P

Y

Y

L

L

G

C

F

F

S

A

A

A

L

L

L

L

M

M

A

A

G

G

L

L

D

D

G

G

V

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

E

E

A

P

A

M

D

D

K

K

N

N

L

L

Y

Y

D

D

L

L

P

P

P

P

E

E

E

E

D

A

A

K

K

E

I

I

P

P

T

Q

V

V

C

A

S

G

S

S

L

L

D

E

Q

E

A

A

L

L

E

N

Y

A

L

L

D

D

N

L

D

D

R

R

E

E

F

F

L

L

T

K

R

A

G

G

G

G

V

V

F

F

S

T

N

D

S

E

M

A

I

I

D

D

A

A

Y

Y

I

I

E

A

L

L

K

R

M

R

E

E

E

E

V

D

T

D

R

R

F

V

R

R

M

M

T

T

T

P

H

H

P

P

I

V

E

E

F

F

D

E

M

L

Y

Y

Y

Y

S

S

L

V

binding adenosine-5'-diphosphate: E129 (= E132), E207 (= E210), H210 (= H213), E220 (= E223), T223 (= T226), F225 (= F228), H271 (= H274), S273 (= S276), R355 (= R358)
binding manganese (ii) ion: E129 (= E132), E131 (= E134), E212 (= E215), E220 (= E223), H269 (= H272), E357 (= E360)

2lgsA Feedback inhibition of fully unadenylylated glutamine synthetase from salmonella typhimurium by glycine, alanine, and serine (see paper)
61% identity, 99% coverage: 4:471/471 of query aligns to 1:445/445 of 2lgsA

query
sites
2lgsA

S

S

V

A

A

E

D

H

V

V

M

L

K

T

L

M

V

L

K

N

E

E

N

H

D

E

V

V

K

K

F

F

V

V

D

D

F

L

R

R

F

F

T

T

D

D

T

T

K

K

G

G

K

K

E

E

Q

Q

H

H

V

V

S

T

V

I

P

P

V

A

S

H

H

Q

F

V

D

N

E

A

D

E

K

F

F

F

E

E

S

E

G

G

H

K

A

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

-

G

-

I

-

E

-

A

-

S

-

D

D

M

M

L

V

L

L

M

M

P

P

D

D

P

A

N

S

T

T

A

A

N

V

V

I

D

D

P

P

F

F

Y

F

E

A

E

D

P

S

T

T

L

L

I

I

L

I

T

R

C

C

D

D

V

I

V

L

E

E

P

P

S

G

D

T

G

L

K

Q

G

G

Y

Y

D

D

R

R

D

D

P

P

R

R

S

S

I

I

A

A

K

K

R

R

A

A

E

E

A

D

Y

Y

L

L

K

R

S

A

T

T

G

G

L

I

G

A

D

D

T

T

A

V

F

L

F

F

G

G

P

P

E
|
E

P

P

E
|
E

F

F

F

F

I

L

F

F

D

D

G

D

V

I

T

R

W

F

N

G

I

A

D

S

M

I

Q

S

G

G

C

S

S

H

V

V

K

A

I

I

H

D

S

D

E

I

E

E

A

G

P

A

W

W

S

N

S

S

G

S

K

T

E

K

F

Y

E

E

H

G

G

G

N

N

S

K

G

G

H

H

R

R

P

P

G

G

K

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

A

P

P

P

I

V

D

D

T

S

F

A

Q

Q

D

D

I

I

R

R

S

S

E

E

M

M

C

C

L

L

I

V

L

M

E

E

S

Q

L

M

G

G

I

L

P

V

V

V

E

E

V

A

H

H

H

H

H

H

E
|
E

V

V

A

A

G

T

Q

A

G

G

Q

Q

N

N

E
|
E

I

V

G

A

T

T

K

R

F

F

S

N

T

T

L

M

V

T

Q

K

R

K

A

A

D

D

W

E

T

I

Q

Q

L

I

Q

Y

K

K

Y

Y

V

V

I

V

Q

H

N

N

V

V

A

A

H

H

T

R

Y

F

G

G

K

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

M

V

F

G

G

D

D

N
|
N

G
|
G

S

S

G
|
G

M

M

H
|
H

V

C

H

H

Q

M

S

S

V

L

W

A

K

K

D

N

G

G

Q

T

N

N

L

L

F

F

A

S

G

G

N

D

G

K

Y

Y

A

A

G

G

L

L

S

S

E

E

F

Q

A

A

L

L

Y

Y

Y

Y

I

I

G

G

G

G

I

V

I

I

K

K

H

H

A

A

R

K

A

A

L

I

N

N

A

A

L

L

T

A

N

N

P

P

G

T

T

T

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

-

F

-

E

-

A

-

P

P

V

V

K

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

A

A

N

S

P

P

K

K

G

A

R

R

R

R

I

I

E
|
E

T

V

R
|
R

F

F

P

P

D

D

P

P

L

A

M

A

N

N

P

P

Y

Y

L

L

G

C

F

F

S

A

A

A

L

L

L

L

M

M

A

A

G

G

L

L

D

D

G

G

V

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

E

E

A

P

A

M

D

D

K

-

N

-

L

-

Y

-

D

-

L

-

P

-

P

-

E

-

E

-

D

-

A

-

K

-

I

I

P

P

T

Q

V

V

C

A

S

G

S

S

L

L

D

E

Q

E

A

A

L

L

E

N

Y

A

L

L

D

D

N

L

D

D

R

R

E

E

F

F

L

L

T

K

R

A

G

G

G

G

V

V

F

F

S

T

N

D

S

E

M

A

I

I

D

D

A

A

Y

Y

I

I

E

A

L

L

K

R

M

R

E

E

E

E

V

D

T

D

R

R

F

V

R

R

M

M

T

T

T

P

H

H

P

P

I

V

E

E

F

F

D

E

M

L

Y

Y

Y

Y

S

S

L

V

binding glutamic acid: E125 (= E134), N258 (= N267), G259 (= G268), G261 (= G270), H263 (= H272), R315 (= R324), R349 (= R362)
binding manganese (ii) ion: E123 (= E132), E125 (= E134), E206 (= E215), E214 (= E223), H263 (= H272), E347 (= E360)

1lgrA Interactions of nucleotides with fully unadenylylated glutamine synthetase from salmonella typhimurium (see paper)
61% identity, 99% coverage: 4:471/471 of query aligns to 1:445/445 of 1lgrA

query
sites
1lgrA

S

S

V

A

A

E

D

H

V

V

M

L

K

T

L

M

V

L

K

N

E

E

N

H

D

E

V

V

K

K

F

F

V

V

D

D

F

L

R

R

F

F

T

T

D

D

T

T

K

K

G

G

K

K

E

E

Q

Q

H

H

V

V

S

T

V

I

P

P

V

A

S

H

H

Q

F

V

D

N

E

A

D

E

K

F

F

F

E

E

S

E

G

G

H

K

A

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

-

G

-

I

-

E

-

A

-

S

-

D

D

M

M

L

V

L

L

M

M

P

P

D

D

P

A

N

S

T

T

A

A

N

V

V

I

D

D

P

P

F

F

Y

F

E

A

E

D

P

S

T

T

L

L

I

I

L

I

T

R

C

C

D

D

V

I

V

L

E

E

P

P

S

G

D

T

G

L

K

Q

G

G

Y

Y

D

D

R

R

D

D

P

P

R

R

S

S

I

I

A

A

K

K

R

R

A

A

E

E

A

D

Y

Y

L

L

K

R

S

A

T

T

G

G

L

I

G

A

D

D

T

T

A

V

F

L

F

F

G

G

P

P

E
|
E

P

P

E
|
E

F

F

F

F

I

L

F

F

D

D

G

D

V

I

T

R

W

F

N

G

I

A

D

S

M

I

Q

S

G

G

C

S

S

H

V

V

K

A

I

I

H

D

S

D

E

I

E

E

A

G

P

A

W

W

S

N

S

S

G

S

K

T

E

K

F

Y

E

E

H

G

G

G

N

N

S

K

G

G

H

H

R

R

P

P

G

G

K

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

A

P

P

P

I

V

D

D

T

S

F

A

Q

Q

D

D

I

I

R

R

S

S

E

E

M

M

C

C

L

L

I

V

L

M

E

E

S

Q

L

M

G

G

I

L

P

V

V

V

E
|
E

V

A

H

H

H

H

H

H

E
|
E

V

V

A

A

G

T

Q

A

G

G

Q

Q

N

N

E
|
E

I

V

G

A

T
|
T

K

R

F
|
F

S

N

T

T

L

M

V

T

Q

K

R

K

A

A

D

D

W

E

T

I

Q

Q

L

I

Q

Y

K

K

Y

Y

V

V

I

V

Q

H

N

N

V

V

A

A

H

H

T

R

Y

F

G

G

K

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

M

V

F

G

G

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

C

H
|
H

Q

M

S
|
S

V

L

W

A

K

K

D

N

G

G

Q

T

N

N

L

L

F

F

A

S

G

G

N

D

G

K

Y

Y

A

A

G

G

L

L

S

S

E

E

F

Q

A

A

L

L

Y

Y

Y

Y

I

I

G

G

G

G

I

V

I

I

K

K

H

H

A

A

R

K

A

A

L

I

N

N

A

A

L

L

T

A

N

N

P

P

G

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

-

F

-

E

-

A

-

P

P

V

V

K

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

A

A

N

S

P

P

K

K

G

A

R

R

R
|
R

I

I

E
|
E

T

V

R

R

F

F

P

P

D

D

P

P

L

A

M

A

N

N

P

P

Y

Y

L

L

G

C

F

F

S

A

A

A

L

L

L

L

M

M

A

A

G

G

L

L

D

D

G

G

V

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

E

E

A

P

A

M

D

D

K

-

N

-

L

-

Y

-

D

-

L

-

P

-

P

-

E

-

E

-

D

-

A

-

K

-

I

I

P

P

T

Q

V

V

C

A

S

G

S

S

L

L

D

E

Q

E

A

A

L

L

E

N

Y

A

L

L

D

D

N

L

D

D

R

R

E

E

F

F

L

L

T

K

R

A

G

G

G

G

V

V

F

F

S

T

N

D

S

E

M

A

I

I

D

D

A

A

Y

Y

I

I

E

A

L

L

K

R

M

R

E

E

E

E

V

D

T

D

R

R

F

V

R

R

M

M

T

T

T

P

H

H

P

P

I

V

E

E

F

F

D

E

M

L

Y

Y

Y

Y

S

S

L

V

binding adenosine monophosphate: E201 (= E210), T217 (= T226), F219 (= F228), H265 (= H274), S267 (= S276), R345 (= R358)
binding manganese (ii) ion: E123 (= E132), E125 (= E134), E206 (= E215), E214 (= E223), H263 (= H272), E347 (= E360)

P77961 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Synechocystis sp. (strain PCC 6803 / Kazusa)
56% identity, 100% coverage: 1:469/471 of query aligns to 1:471/473 of P77961

query
sites
P77961

M

M

A

A

H

R

S

T

V

P

A

Q

D

E

V

V

M

L

K

K

L

W

V

I

K

Q

E

D

N

E

D

N

V

I

K

K

F

I

V

I

D

D

F

L

R

K

F

F

T

I

D

D

T

T

K

P

G

G

K

I

E

W

Q

Q

H

H

V

C

S

S

V

F

P

Y

V

Y

S

D

H

Q

F

L

D

D

E

E

D

N

K

S

F

F

E

T

S

E

G

G

H

I

A

P

F

F

D

D

G

G

S

S

S

S

I

I

A

R

G

G

W

W

K

K

G

A

I

I

E

N

A

E

S

S

D

D

M

M

L

C

L

M

M

V

P

P

D

D

P

P

N

N

T

T

A

A

N

T

V

I

D

D

P

P

F

F

Y

C

E

K

E

E

P

P

T

T

L

L

I

S

L

M

T

I

C

C

D

S

V

I

V

K

E

E

P

P

S

R

D

T

G

G

K

E

G

W

Y

Y

D

N

R

R

D

D

P

P

R

R

S

T

I

I

A

A

K

A

R

K

A

A

E

V

A

E

Y

Y

L

L

K

R

S

G

T

T

G

G

L

I

G

A

D

D

T

T

A

V

F

Y

F

F

G

G

P

P

E

E

P

A

E
|
E

F

F

F

F

I

L

F

F

D

D

G

D

V

I

T

R

W

F

N

G

I

Q

D

T

M

E

Q

N

G

S

C

S

S

Y

V

Y

K

F

I

A

H

D

S

S

E

V

E

E

A

G

P

R

W

W

S

N

S

T

G

G

K

R

E

E

F

E

E

E

H

G

G

G

N

N

S

L

G

G

H

Y

R

K

P

P

G

G

K

Y

K

K

G

Q

G

G

Y

Y

F

F

P

P

V

V

A

A

P

P

I

T

D

D

T

T

F

A

Q

Q

D

D

I

I

R

R

S

T

E

E

M

M

C

L

L

L

I

T

L

M

E

A

S

G

L

L

G

C

I

V

P

P

V

I

E

E

V

K

H

H

H

H

H

H

E
|
E

V

V

A

A

G

S

Q

G

G

G

Q

Q

N

N

E
|
E

I

L

G

G

T

I

K

K

F

F

S

D

T

K

L

L

V

V

Q

N

R

S

A

A

D

D

W

N

T

L

Q

M

L

I

Q

Y

K

K

Y

Y

V

V

I

I

Q

K

N

N

V

V

A

A

H

K

T

K

Y

Y

G

G

K

K

T

T

A

V

T

T

F

F

M

M

P

P

K

K

P

P

V

I

V

F

G

N

D

D

N

N

G

G

S

S

G

G

M

M

H

H

V

V

H

H

Q

Q

S

S

V

L

W

W

K

K

D

D

G

G

Q

Q

N

P

L

L

F

F

A

A

G

G

N

D

G

K

Y

Y

A

A

G

G

L

F

S

S

E

Q

F

M

A

G

L

L

Y

W

Y

Y

I

I

G

G

G

G

I

I

I

L

K

K

H

H

A

A

R

P

A

A

L

L

N

L

A

A

L

F

T

T

N

N

P

P

G

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

F

E

E

A

A

P

P

V

V

K

N

L

L

A

A

Y

Y

S

S

A

Q

R

G

N

N

R

R

S

S

A

A

S

S

I

V

R

R

I

I

P

P

Y

L

V

S

-

G

A

G

N

N

P

P

K

K

G

A

R

K

R

R

I

L

E
|
E

T

F

R

R

F

C

P

P

D

D

P

A

L

T

M

S

N

N

P

P

Y

Y

L

L

G

A

F

F

S

A

A

A

L

M

L

L

M

C

A

A

G

G

L

I

D

D

G

G

V

I

Q

K

N

N

K

Q

I

I

H

D

P

P

G

G

E

E

A

P

A

L

D

D

K

V

N

D

L

I

Y

Y

D

D

L

L

P

S

P

P

E

E

E

E

D

L

A

A

K

K

I

I

P

P

T

S

V

T

C

P

S

G

S

S

L

L

D

E

Q

A

A

A

L

L

E

E

Y

A

L

L

D

E

N

K

D

D

R

H

E

E

F

F

L

L

T

T

R

G

G

T

G

G

V

V

F

F

S

S

N

P

S

D

M

F

I

V

D

E

A

S

Y

W

I

I

E

E

L

Y

K

K

M

L

E

D

-

N

E

E

V

V

T

N

R

P

F

M

R

R

M

L

T

R

T

P

H

H

P

P

I

Y

E

E

F

F

D

S

M

L

Y

Y

Y

Y

E134 (= E134) binding Mg(2+)
E215 (= E215) binding Mg(2+)
E223 (= E223) binding Mg(2+)
E361 (= E360) binding Mn(2+)

3ng0A Crystal structure of glutamine synthetase from synechocystis sp. Pcc 6803
55% identity, 98% coverage: 7:469/471 of query aligns to 5:463/465 of 3ng0A

query
sites
3ng0A

D

E

V

V

M

L

K

K

L

W

V

I

K

Q

E

D

N

E

D

N

V

I

K

K

F

I

V

I

D

D

F

L

R

K

F

F

T

I

D

D

T

T

K

P

G

G

K

I

E

W

Q

Q

H

H

V

C

S

S

V

F

P

Y

V

Y

S

D

H

Q

F

L

D

D

E

E

D

N

K

S

F

F

E

T

S

E

G

G

H

I

A

P

F

F

D
|
D

G

G

S

S

S

S

I

I

A

R

G

G

W

W

K

K

G

A

I

I

E

N

A

E

S

S

D

D

M

M

L

C

L

M

M

V

P

P

D

D

P

P

N

N

T

T

A

A

N

T

V

I

D

D

P

P

F

F

Y

C

E

K

E

E

P

P

T

T

L

L

I

S

L

M

T

I

C

C

D

S

V

I

V

K

E

E

P

P

S

R

D

T

G

G

K

E

G

W

Y

Y

D

N

R

R

D

D

P

P

R

R

S

T

I

I

A

A

K

A

R

K

A

A

E

A

A

E

Y

Y

L

L

K

R

S

G

T

T

G

G

L

I

G

A

D

D

T

T

A

V

F
x
Y

F

F

G

G

P

P

E
|
E

P

A

E
|
E

F

F

F

F

I

L

F

F

D

D

G

D

V

I

T

R

W

F

N

G

I

Q

D

T

M

E

Q

N

G

S

C

S

S

Y

V

Y

K

F

I

A

H

D

S

S

E

V

E

E

A

G

P

R

W

W

S

N

S

T

G

G

K

R

E

E

F

E

E

E

H

G

G

G

N

N

S

L

G

G

H

Y

R

K

P

P

G

G

K

Y

K

K

G

Q

G

G

Y

Y

F

F

P

P

V

V

A

A

P

P

I

T

D

D

T

T

F

A

Q

Q

D

D

I

I

R

R

S

T

E

E

M

M

C

L

L

L

I

T

L

M

E

A

S

A

L

F

G

G

I

V

P

P

V

I

E

E

V
x
K

H

H

H

H

H

H

E
|
E

V

V

A

A

G

S

Q

G

G

G

Q

Q

N

N

E
|
E

I

L

G

G

T
x
I

K

K

F
|
F

S

D

T

K

L

L

V

V

Q

N

R

S

A

A

D

D

W

N

T

L

Q

M

L

I

Q

Y

K

K

Y

Y

V

V

I

I

Q

K

N

N

V

V

A

A

H

K

T

K

Y

Y

G

G

K

K

T

T

A

V

T

T

F

F

M

M

P

P

K

K

P

P

V

I

V

F

G

N

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

V

H
|
H

Q

Q

S
|
S

V

L

W

W

K

K

D

D

G

G

Q

Q

N

P

L

L

F

F

A

A

G

G

N

D

G

K

Y

Y

A

A

G

G

L

F

S

S

E

Q

F

M

A

G

L

L

Y

W

Y

Y

I

I

G

G

G

G

I

I

I

L

K

K

H

H

A

A

R

P

A

A

L

L

N

L

A

A

L

F

T

T

N

N

P

P

G

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

F

E

E

A

A

P

P

V

V

K

N

L

L

A

A

Y

Y

S

S

A

Q

R

G

N

N

R
|
R

S

S

A

A

S

S

I

V

R
|
R

I

I

P

P

Y

L

V

S

-

G

A

G

N

N

P

P

K

K

G

A

R

K

R
|
R

I

L

E
|
E

T

F

R
|
R

F

C

P

P

D

D

P

A

L

T

M

S

N

N

P

P

Y

Y

L

L

G

A

F

F

S

A

A

A

L

M

L

L

M

C

A

A

G

G

L

I

D

D

G

G

V

I

Q

K

N

N

K

Q

I

I

H

D

P

P

G

G

E

E

A

P

A

L

D

D

K

V

N

D

L

I

Y

-

D

-

L

-

P

-

P

-

E

-

E

E

D

L

A

A

K

K

I

I

P

P

T

S

V

T

C

P

S

G

S

S

L

L

D

E

Q

A

A

A

L

L

E

E

Y

A

L

L

D

E

N

K

D

D

R

H

E

E

F

F

L

L

T

T

R

G

G

T

G

G

V

V

F

F

S

S

N

P

S

D

M

F

I

V

D

E

A

S

Y

W

I

I

E

E

L

Y

K

K

M

L

E

D

-

N

E

E

V

V

T

N

R

P

F

M

R

R

M

L

T

R

T

P

H

H

P

P

I

Y

E

E

F

F

D

S

M

L

Y

Y

Y

Y

active site: D51 (= D53), E130 (= E132), E132 (= E134), E213 (= E215), E221 (= E223), H270 (= H272), R340 (= R342), E359 (= E360), R361 (= R362)
binding phosphoaminophosphonic acid-adenylate ester: Y126 (≠ F128), E130 (= E132), K209 (≠ V211), I224 (≠ T226), F226 (= F228), H272 (= H274), S274 (= S276), R340 (= R342), R345 (= R347), R357 (= R358)
binding manganese (ii) ion: E130 (= E132), E132 (= E134), E213 (= E215), E221 (= E223), H270 (= H272), E359 (= E360), R361 (= R362)

A0R079 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
52% identity, 99% coverage: 7:471/471 of query aligns to 8:478/478 of A0R079

query
sites
A0R079

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K14 (= K13) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

1htoA Crystallographic structure of a relaxed glutamine synthetase from mycobacterium tuberculosis (see paper)
51% identity, 99% coverage: 7:471/471 of query aligns to 7:477/477 of 1htoA

query
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1htoA

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