SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS14320 FitnessBrowser__Cup4G11:RR42_RS14320 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WGB5 O-succinylhomoserine sulfhydrylase; OSH sulfhydrylase; OSHS sulfhydrylase; EC 2.5.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
51% identity, 99% coverage: 5:399/401 of query aligns to 13:405/406 of P9WGB5

query
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P9WGB5

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K219 (= K212) modified: N6-(pyridoxal phosphate)lysine

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
49% identity, 91% coverage: 30:395/401 of query aligns to 27:388/396 of 4omaA

query
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active site: R59 (= R62), Y112 (≠ F115), D184 (= D187), K209 (= K212)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G90), I88 (≠ M91), Y112 (≠ F115), D184 (= D187), S206 (= S209), T208 (= T211), K209 (= K212), V337 (≠ G344), S338 (≠ N345), R373 (= R380)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
49% identity, 91% coverage: 30:395/401 of query aligns to 27:388/396 of 3jwbA

query
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active site: R59 (= R62), Y112 (≠ F115), D184 (= D187), K209 (= K212)
binding norleucine: Y112 (≠ F115), K209 (= K212), S338 (≠ N345), R373 (= R380)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
49% identity, 91% coverage: 30:395/401 of query aligns to 27:388/396 of 3jwaA

query
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3jwaA

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L

P

A

E

A

E

W

I

R

R

A

A

A

A

V

M

K

R

P

P

N

E

T

T

K

K

L

V

F

V

F

Y

L

I

E

E

T

T

P

P

S

A

N

N

P

P

L

T

T

L

E

S

V

L

A

V

D

D

I

I

A

E

A

T

V

V

A

A

D

G

I

I

A

A

H

H

N

Q

A

Q

G

G

A

A

L

L

L

L

V

V

V

V

D
|
D

N

N

C

T

F

F

C

M

S

S

P

P

A

Y

L

C

Q

Q

Q

Q

P

P

M

L

K

Q

F

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S

S

A

V

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

V

V

L

I

G

G

G

G

A

I

V

I

L

V

G

G

S

K

H

Q

D

E

F

F

I

I

M

D

G

Q

K

A

V

R

F

F

P

V

F

G

V

L

R

K

-

D

T

I

A

T

G

G

P

G

T

C

L

M

S

S

A

P

F

F

N

N

A

A

W

W

V

L

L

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

A

G

I

I

R

R

M

M

E

E

R

R

H

H

S

C

Q

E

S

N

A

A

L

L

A

K

L

I

A

A

E

R

F

F

L

L

E

E

S

G

H

H

P

P

A

S

V

I

A

T

R

R

V

V

Y

Y

H

Y

P

P

A

G

L

L

K

S

S

S

H

H

P

P

Q

Q

Y

Y

E

E

V

L

A

G

Q

Q

R

R

Q

Q

Q

M

S

S

G

L

G

P

G

G

A

G

I

I

V

I

S

S

F

F

E

E

L

I

K

A

G

G

D

G

T

L

P

-

E

E

Q

A

Q

G

R

R

A

-

N

-

A

-

W

-

R

R

V

M

I

I

D

N

N

S

T

V

R

E

V

L

C

C

S

L

I

L

T

A

G
x
V

N
x
S

L

L

G

G

D

D

T

T

R

E

T

T

T

L

V

I

T

Q

H

H

P

P

Y

A

T

S

T

M

T

T

H

H

G

S

R

P

V

V

S

A

P

P

Q

E

A

E

K

R

A

L

A

K

A

A

G

G

I

I

S

T

E

D

G

G

L

L

I

I

R
|
R

L

L

A

S

V

V

G

G

L

L

E

E

S

D

V

P

A

E

D

D

L

I

K

I

A

N

D

D

L

L

active site: R59 (= R62), Y112 (≠ F115), D184 (= D187), K209 (= K212)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (≠ F115), K209 (= K212), V337 (≠ G344), S338 (≠ N345), R373 (= R380)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
49% identity, 91% coverage: 30:395/401 of query aligns to 27:388/396 of 3jw9A

query
sites
3jw9A

S

S

E

T

A

P

M

I

Y

F

L

Q

T

T

S

S

S

T

F

F

C

V

F

F

N

D

S

S

A

A

A

E

E

Q

A

G

A

A

E

A

R

R

F

F

A

A

N

L

S

E

E

E

S

S

G

G

F

Y

T

I

Y

Y

S

T

R
|
R

F

L

T

G

N

N

P

P

T

T

V

T

A

D

M

A

F

L

Q

E

S

K

R

K

L

L

A

A

A

V

L

L

E

E

G

R

A

G

E

E

A

A

C

G

M

L

A

A

T

T

A

A

S

S

G

G

M

I

S

S

A

A

I

I

M

T

S

T

I

T

V

L

M

L

S

T

A

L

M

C

Q

Q

A

Q

G

G

D

D

H

H

L

I

V

V

S

S

S

A

R

S

A

A

I

I

F
x
Y

G

G

S

C

T

T

M

H

T

A

L

F

F

L

S

S

N

H

I

S

F

M

A

P

K

K

F

F

G

G

V

I

E

N

T

V

T

S

F

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

A

E

W

I

R

R

A

A

A

A

V

M

K

R

P

P

N

E

T

T

K

K

L

V

F

V

F

Y

L

I

E

E

T

T

P

P

S

A

N

N

P

P

L

T

T

L

E

S

V

L

A

V

D

D

I

I

A

E

A

T

V

V

A

A

D

G

I

I

A

A

H

H

N

Q

A

Q

G

G

A

A

L

L

L

L

V

V

V

V

D
|
D

N

N

C

T

F

F

C

M

S

S

P

P

A

Y

L

C

Q

Q

Q

Q

P

P

M

L

K

Q

F

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S

S

A

V

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

V

V

L

I

G

G

G

G

A

I

V

I

L

V

G

G

S

K

H

Q

D

E

F

F

I

I

M

D

G

Q

K

A

V

R

F

F

P

V

F

G

V

L

R

K

-

D

T

I

A

T

G

G

P

G

T

C

L

M

S

S

A

P

F

F

N

N

A

A

W

W

V

L

L

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

A

G

I

I

R

R

M

M

E

E

R

R

H

H

S

C

Q

E

S

N

A

A

L

L

A

K

L

I

A

A

E

R

F

F

L

L

E

E

S

G

H

H

P

P

A

S

V

I

A

T

R

R

V

V

Y

Y

H

Y

P

P

A

G

L

L

K

S

S

S

H

H

P

P

Q

Q

Y

Y

E

E

V

L

A

G

Q

Q

R

R

Q

Q

Q

M

S

S

G

L

G

P

G

G

A

G

I

I

V

I

S

S

F

F

E

E

L

I

K

A

G

G

D

G

T

L

P

-

E

E

Q

A

Q

G

R

R

A

-

N

-

A

-

W

-

R

R

V

M

I

I

D

N

N

S

T

V

R

E

V

L

C

C

S

L

I

L

T

A

G
x
V

N
x
S

L

L

G

G

D

D

T

T

R

E

T

T

T

L

V

I

T

Q

H

H

P

P

Y

A

T

S

T

M

T

T

H

H

G

S

R

P

V

V

S

A

P

P

Q

E

A

E

K

R

A

L

A

K

A

A

G

G

I

I

S

T

E

D

G

G

L

L

I

I

R

R

L

L

A

S

V

V

G

G

L

L

E

E

S

D

V

P

A

E

D

D

L

I

K

I

A

N

D

D

L

L

active site: R59 (= R62), Y112 (≠ F115), D184 (= D187), K209 (= K212)
binding S-ethyl-L-cysteine: Y112 (≠ F115), K209 (= K212), V337 (≠ G344), S338 (≠ N345)

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
49% identity, 91% coverage: 30:395/401 of query aligns to 26:387/395 of 5m3zA

query
sites
5m3zA

S

S

E

T

A

P

M

I

Y

F

L

Q

T

T

S

S

S

T

F

F

C

V

F

F

N

D

S

S

A

A

A

E

E

Q

A

G

A

A

E

A

R

R

F

F

A

A

N

L

S

E

E

E

S

S

G

G

F

Y

T

I

Y

Y

S

T

R
|
R

F

L

T

G

N

N

P

P

T

T

V

T

A

D

M

A

F

L

Q

E

S

K

R

K

L

L

A

A

A

V

L

L

E

E

G

R

A

G

E

E

A

A

C

G

M

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

M

T

S

T

I

T

V

L

M

L

S

T

A

L

M

C

Q

Q

A

Q

G

G

D

D

H

H

L

I

V

V

S

S

S

A

R

S

A

A

I

I

F
x
Y

G

G

S
x
H

T

T

M

H

T

A

L

F

F

L

S

S

N

H

I

S

F

M

A

P

K

K

F

F

G

G

V

I

E

N

T

V

T

S

F

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

A

E

W

I

R

R

A

A

A

A

V

M

K

R

P

P

N

E

T

T

K

K

L

V

F

V

F

Y

L

I

E
|
E

T

T

P

P

S

A

N

N

P

P

L

T

T

L

E

S

V

L

A

V

D

D

I

I

A

E

A

T

V

V

A

A

D

G

I

I

A

A

H

H

N

Q

A

Q

G

G

A

A

L

L

L

L

V

V

V

V

D
|
D

N

N

C
x
T

F

F

C

M

S

S

P

P

A

Y

L

C

Q

Q

Q

Q

P

P

M

L

K

Q

F

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

V

V

L

I

G

G

G

G

A

I

V

I

L

V

G

G

S

K

H

Q

D

E

F

F

I

I

M

D

G

Q

K

A

V

R

F

F

P

V

F

G

V

L

R

K

-

D

T

I

A

T

G

G

P

G

T

C

L

M

S

S

A

P

F

F

N

N

A

A

W

W

V

L

L

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

A

G

I

I

R

R

M

M

E

E

R

R

H

H

S

C

Q

E

S

N

A

A

L

L

A

K

L

I

A

A

E

R

F

F

L

L

E

E

S

G

H

H

P

P

A

S

V

I

A

T

R

R

V

V

Y

Y

H

Y

P

P

A

G

L

L

K

S

S

S

H

H

P

P

Q

Q

Y

Y

E

E

V

L

A

G

Q

Q

R

R

Q

Q

Q

M

S

S

G

L

G

P

G

G

A

G

I

I

V

I

S

S

F

F

E

E

L

I

K

A

G

G

D

G

T

L

P

-

E

E

Q

A

Q

G

R

R

A

-

N

-

A

-

W

-

R

R

V

M

I

I

D

N

N

S

T

V

R

E

V

L

C

C

S

L

I

L

T

A

G
x
V

N
x
S

L

L

G

G

D

D

T

T

R

E

T

T

T

L

V

I

T

Q

H

H

P

P

Y

A

T

S

T

M

T

T

H

H

G

S

R

P

V

V

S

A

P

P

Q

E

A

E

K

R

A

L

A

K

A

A

G

G

I

I

S

T

E

D

G

G

L

L

I

I

R
|
R

L

L

A

S

V

V

G

G

L

L

E

E

S

D

V

P

A

E

D

D

L

I

K

I

A

N

D

D

L

L

active site: R58 (= R62), Y111 (≠ F115), D183 (= D187), K208 (= K212)
binding norleucine: Y111 (≠ F115), H113 (≠ S117), K208 (= K212), V336 (≠ G344), S337 (≠ N345)
binding pyridoxal-5'-phosphate: G86 (= G90), I87 (≠ M91), Y111 (≠ F115), E154 (= E158), D183 (= D187), T185 (≠ C189), S205 (= S209), T207 (= T211), K208 (= K212)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G90), I87 (≠ M91), Y111 (≠ F115), D183 (= D187), S205 (= S209), T207 (= T211), K208 (= K212), V336 (≠ G344), S337 (≠ N345), R372 (= R380)

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
49% identity, 91% coverage: 30:395/401 of query aligns to 27:388/396 of 4hf8A

query
sites
4hf8A

S

S

E

T

A

P

M

I

Y

F

L

Q

T

T

S

S

S

T

F

F

C

V

F

F

N

D

S

S

A

A

A

E

E

Q

A

G

A

A

E

A

R

R

F

F

A

A

N

L

S

E

E

E

S

S

G

G

F

Y

T

I

Y

Y

S

T

R
|
R

F

L

T

G

N

N

P

P

T

T

V

T

A

D

M

A

F

L

Q

E

S

K

R

K

L

L

A

A

A

V

L

L

E

E

G

R

A

G

E

E

A

A

C

G

M

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

M

T

S

T

I

T

V

L

M

L

S

T

A

L

M

C

Q

Q

A

Q

G

G

D

D

H

H

L

I

V

V

S

S

S

A

R

S

A

A

I

I

F
x
Y

G

G

S

C

T

T

M

H

T

A

L

F

F

L

S

S

N

H

I

S

F

M

A

P

K

K

F

F

G

G

V

I

E

N

T

V

T

R

F

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

A

E

W

I

R

R

A

A

A

A

V

M

K

R

P

P

N

E

T

T

K

K

L

V

F

V

F

Y

L

I

E
|
E

T

T

P

P

S

A

N
|
N

P

P

L

T

T

L

E

S

V

L

A

V

D

D

I

I

A

E

A

T

V

V

A

A

D

G

I

I

A

A

H

H

N

Q

A

Q

G

G

A

A

L

L

L

L

V

V

V

V

D
|
D

N

N

C

T

F

F

C

M

S

S

P

P

A

Y

L

C

Q

Q

Q

Q

P

P

M

L

K

Q

F

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

V

V

L

I

G

G

G

G

A

I

V

I

L

V

G

G

S

K

H

Q

D

E

F

F

I

I

M

D

G

Q

K

A

V

R

F

F

P

V

F

G

V

L

R

K

-

D

T

I

A

T

G

G

P

G

T

C

L

M

S

S

A

P

F

F

N

N

A

A

W

W

V

L

L

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

A

G

I

I

R

R

M

M

E

E

R

R

H

H

S

C

Q

E

S

N

A

A

L

L

A

K

L

I

A

A

E

R

F

F

L

L

E

E

S

G

H

H

P

P

A

S

V

I

A

T

R

R

V

V

Y

Y

H

Y

P

P

A

G

L

L

K

S

S

S

H

H

P

P

Q

Q

Y

Y

E

E

V

L

A

G

Q

Q

R

R

Q

Q

Q

M

S

S

G

L

G

P

G

G

A

G

I

I

V

I

S

S

F

F

E

E

L

I

K

A

G

G

D

G

T

L

P

-

E

E

Q

A

Q

G

R

R

A

-

N

-

A

-

W

-

R

R

V

M

I

I

D

N

N

S

T

V

R

E

V

L

C

C

S

L

I

L

T

A

G

V

N
x
S

L

L

G

G

D

D

T

T

R

E

T

T

T

L

V

I

T

Q

H

H

P

P

Y

A

T

S

T

M

T

T

H

H

G

S

R

P

V

V

S

A

P

P

Q

E

A

E

K

R

A

L

A

K

A

A

G

G

I

I

S

T

E

D

G

G

L

L

I

I

R
|
R

L

L

A

S

V

V

G

G

L

L

E

E

S

D

V

P

A

E

D

D

L

I

K

I

A

N

D

D

L

L

active site: R59 (= R62), Y112 (≠ F115), D184 (= D187), K209 (= K212)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G90), I88 (≠ M91), Y112 (≠ F115), E155 (= E158), N159 (= N162), D184 (= D187), S206 (= S209), K209 (= K212), S338 (≠ N345), R373 (= R380)

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
48% identity, 91% coverage: 30:395/401 of query aligns to 27:388/396 of 6egrA

query
sites
6egrA

S

S

E

T

A

P

M

I

Y

F

L

Q

T

T

S

S

S

T

F

F

C

V

F

F

N

D

S

S

A

A

A

E

E

Q

A

G

A

A

E

A

R

R

F

F

A

A

N

L

S

E

E

E

S

S

G

G

F

Y

T

I

Y

Y

S

T

R

R

F

L

T

G

N

N

P

P

T

T

V

T

A

D

M

A

F

L

Q

E

S

K

R

K

L

L

A

A

A

V

L

L

E

E

G

R

A

G

E

E

A

A

C

G

M

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

M

T

S

T

I

T

V

L

M

L

S

T

A

L

M

C

Q

Q

A

Q

G

G

D

D

H

H

L

I

V

V

S

S

S

A

R

S

A

A

I

I

F
x
Y

G

G

S

C

T

T

M

H

T

A

L

F

F

L

S

S

N

H

I

S

F

M

A

P

K

K

F

F

G

G

V

I

E

N

T

V

T

S

F

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

A

E

W

I

R

R

A

A

A

A

V

M

K

R

P

P

N

E

T

T

K

K

L

V

F

V

F

Y

L

I

E
|
E

T

T

P

P

S

A

N

N

P

P

L

T

T

L

E

S

V

L

A

V

D

D

I

I

A

E

A

T

V

V

A

A

D

G

I

I

A

A

H

H

N

Q

A

Q

G

G

A

A

L

L

L

L

V

V

V

V

D
|
D

N

N

C
x
T

F
|
F

C

M

S

S

P

P

A

Y

L

C

Q

Q

Q

Q

P

P

M

L

K

Q

F

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

V

V

L

I

G

G

G

G

A

I

V

I

L

V

G

G

S

K

H

Q

D

E

F

F

I

I

M

D

G

Q

K

A

V

R

F

F

P

V

F

G

V

L

R

K

-

D

T

I

A

T

G

G

P

G

T

C

L

M

S

S

A

P

F

F

N

N

A

A

W

W

V

L

L

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

A

G

I

I

R

R

M

M

E

E

R

R

H

H

S

C

Q

E

S

N

A

A

L

L

A

K

L

I

A

A

E

R

F

F

L

L

E

E

S

G

H

H

P

P

A

S

V

I

A

T

R

R

V

V

Y

Y

H

Y

P

P

A

G

L

L

K

S

S

S

H

H

P

P

Q

Q

Y

Y

E

E

V

L

A

G

Q

Q

R

R

Q

Q

Q

M

S

S

G

L

G

P

G

G

A

G

I

I

V

I

S

S

F

F

E

E

L

I

K

A

G

G

D

G

T

L

P

-

E

E

Q

A

Q

G

R

R

A

-

N

-

A

-

W

-

R

R

V

M

I

I

D

N

N

S

T

V

R

E

V

L

C

C

S

L

I

L

T

A

G

V

N

S

L

L

G

G

D

D

T

T

R

E

T

T

T

L

V

I

T

Q

H

H

P

P

Y

A

T

S

T

M

T

T

H

H

G

S

R

P

V

Y

S

A

P

P

Q

E

A

E

K

R

A

L

A

K

A

A

G

G

I

I

S

T

E

D

G

G

L

L

I

I

R

R

L

L

A

S

V

V

G

G

L

L

E

E

S

D

V

P

A

E

D

D

L

I

K

I

A

N

D

D

L

L

binding pyridoxal-5'-phosphate: G87 (= G90), I88 (≠ M91), Y112 (≠ F115), E155 (= E158), D184 (= D187), T186 (≠ C189), F187 (= F190), S206 (= S209), T208 (= T211), K209 (= K212)

3mkjA Methionine gamma-lyase from citrobacter freundii with pyridoximine-5'- phosphate (see paper)
47% identity, 91% coverage: 30:395/401 of query aligns to 27:377/386 of 3mkjA

query
sites
3mkjA

S

S

E

T

A

P

M

I

Y

F

L

Q

T

T

S

S

S

T

F

F

C

V

F

F

N

D

S

S

A

A

A

E

E

Q

A

G

A

A

E

A

R

R

F

F

A

-

N

-

S

-

E

-

S

-

G

-

F

-

T

-

Y

-

S

-

R

-

F

L

T

G

N

N

P

P

T

T

V

T

A

D

M

A

F

L

Q

E

S

K

R

K

L

L

A

A

A

V

L

L

E

E

G

R

A

G

E

E

A

A

C

G

M

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

M

T

S

T

I

T

V

L

M

L

S

T

A

L

M

C

Q

Q

A

Q

G

G

D

D

H

H

L

I

V

V

S

S

S

A

R

S

A

A

I

I

F
x
Y

G

G

S

C

T

T

M

H

T

A

L

F

F

L

S

S

N

H

I

S

F

M

A

P

K

K

F

F

G

G

V

I

E

N

T

V

T

S

F

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

A

E

W

I

R

R

A

A

A

A

V

M

K

R

P

P

N

E

T

T

K

K

L

V

F

V

F

Y

L

I

E
|
E

T

T

P

P

S

A

N

N

P

P

L

T

T

L

E

S

V

L

A

V

D

D

I

I

A

E

A

T

V

V

A

A

D

G

I

I

A

A

H

H

N

Q

A

Q

G

G

A

A

L

L

L

L

V

V

V

V

D
|
D

N

N

C

T

F
|
F

C

M

S

S

P

P

A

Y

L

C

Q

Q

Q

Q

P

P

M

L

K

Q

F

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

V

V

L

I

G

G

G

G

A

I

V

I

L

V

G

G

S

K

H

Q

D

E

F

F

I

I

M

D

G

Q

K

A

V

R

F

F

P

V

F

G

V

L

R

K

-

D

T

I

A

T

G

G

P

G

T

C

L

M

S

S

A

P

F

F

N

N

A

A

W

W

V

L

L

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

A

G

I

I

R

R

M

M

E

E

R

R

H

H

S

C

Q

E

S

N

A

A

L

L

A

K

L

I

A

A

E

R

F

F

L

L

E

E

S

G

H

H

P

P

A

S

V

I

A

T

R

R

V

V

Y

Y

H

Y

P

P

A

G

L

L

K

S

S

S

H

H

P

P

Q

Q

Y

Y

E

E

V

L

A

G

Q

Q

R

R

Q

Q

Q

M

S

S

G

L

G

P

G

G

A

G

I

I

V

I

S

S

F

F

E

E

L

I

K

A

G

G

D

G

T

L

P

-

E

E

Q

A

Q

G

R

R

A

-

N

-

A

-

W

-

R

R

V

M

I

I

D

N

N

S

T

V

R

E

V

L

C

C

S

L

I

L

T

A

G

V

N

S

L

L

G

G

D

D

T

T

R

E

T

T

T

L

V

I

T

Q

H

H

P

P

Y

A

T

S

T

M

T

T

H

H

G

S

R

P

V

V

S

A

P

P

Q

E

A

E

K

R

A

L

A

K

A

A

G

G

I

I

S

T

E

D

G

G

L

L

I

I

R

R

L

L

A

S

V

V

G

G

L

L

E

E

S

D

V

P

A

E

D

D

L

I

K

I

A

N

D

D

L

L

active site: Y101 (≠ F115), D173 (= D187), K198 (= K212)
binding [5-hydroxy-4-(iminomethyl)-6-methyl-pyridin-3-yl]methyl dihydrogen phosphate: G76 (= G90), I77 (≠ M91), Y101 (≠ F115), E144 (= E158), D173 (= D187), F176 (= F190), S195 (= S209), T197 (= T211), K198 (= K212)

5dx5A Crystal structure of methionine gamma-lyase from clostridium sporogenes (see paper)
41% identity, 98% coverage: 7:399/401 of query aligns to 3:394/399 of 5dx5A

query
sites
5dx5A

L

I

D

K

S

K

L

M

G

G

I

F

D

A

T

T

L

K

G

A

V

I

R

H

A

G

G

G

T

H

M

I

-

G

R

D

S

K

E

Q

F

F

M

G

E

S

H

L

S

A

E

T

A

P

M

I

Y

Y

L

Q

T

T

S

S

S

T

F

F

C

I

F

F

N

D

S

S

A

A

A

E

E

Q

A

G

A

G

E

R

R

R

F

F

A

A

N

G

S

E

E

E

S

S

G

G

F

Y

T

I

Y
|
Y

S

S

R
|
R

F

L

T

G

N

N

P

P

T

T

V

S

A

T

M

E

F

V

Q

E

S

N

R

K

L

L

A

A

A

L

L

L

E

E

G

G

A

G

E

E

A

A

C

A

M

V

A

V

T

A

A

A

S
|
S

G
|
G

M
|
M

S

G

A

A

I

I

M

A

S

A

I

S

V

L

M

W

S

S

A

A

M

L

Q

K

A

S

G

G

D

D

H

H

L

V

V

V

S

A

S

S

R

D

A

T

I

L

F
x
Y

G

G

S

C

T

T

M

F

T

A

L

L

F

L

S

N

N

H

I

G

F

L

A

T

K

R

F

Y

G

G

V

V

E

E

T

V

T

T

F

F

V

V

D

D

G

V

T

S

D

N

L

L

A

D

A

E

W

V

R

K

A

N

A

A

V

L

K

K

P

P

N

N

T

T

K

K

L

V

F

V

F

Y

L

L

E

E

T

T

P

P

S

A

N

N

P

P

L

T

T

L

E

K

V

V

A

T

D

D

I

I

A

R

A

K

V

I

A

S

D

N

I

M

A

V

H

H

-

E

-

S

N

N

A

K

G

E

A

C

L

F

L

V

V

F

V

V

D
|
D

N

N

C

T

F
|
F

C

C

S

T

P

P

A

Y

L

I

Q

Q

Q

R

P

P

M

L

K

E

F

L

G

G

A

A

D

D

I

V

V

V

V

V

H

H

S
|
S

A

A

T
|
T

K
|
K

H

Y

I

L

D

N

G

G

Q

H

G

G

R

D

V

V

L

I

G

A

G

G

A

F

V

A

L

V

G

G

S

K

H

E

D

E

F

F

I

I

-

N

M

Q

G

V

K

K

V

L

F

F

P

G

F

I

V

K

R

D

T

M

A

T

G

G

P

S

T

V

L

T

S

G

A

P

F

F

N

E

A

S

W

F

V

L

L

I

L

I

K

R

G

G

M

M

E

K

T

T

L

L

A

Q

I

L

R

R

M

M

E

E

R

K

H

H

S

C

Q

K

S

N

A

A

L

M

A

E

L

V

A

A

E

K

F

F

L

L

E

E

S

S

H

H

P

P

A

A

V

V

A

E

R

K

V

V

Y

Y

H

Y

P

P

A

G

L

L

K

E

S

S

H

F

P

K

Q

Y

Y

Y

E

Q

V

L

A

A

Q

R

R

E

Q

Q

Q

M

S

K

G

L

G

P

G

G

A

A

I

M

V

I

S

S

F

F

E

E

L

L

K

K

G

G

D

G

T

V

P

E

E

E

Q

G

Q

K

R

-

A

-

N

-

A

-

W

-

R

I

V

V

I

M

D

N

N

N

T

V

R

K

V

L

C

A

S

T

I

L

T

A

G

V

N

S

L

L

G

G

D

D

T

S

R

E

T

T

T

L

V

I

T

Q

H

H

P

P

Y

A

T

S

T

M

T

T

H

H

G

S

R

P

V

Y

S

T

P

A

Q

E

A

E

K

R

A

K

A

A

A

A

G

G

I

I

S

S

E

D

G

G

L

L

I

V

R

R

L

L

A

S

V

V

G

G

L

L

E

E

S

D

V

A

A

E

D

D

L

I

K

I

A

D

D

D

L

L

L

K

R

Q

G

A

L

L

active site: R59 (= R62), Y112 (≠ F115), D186 (= D187), K211 (= K212)
binding pyridoxal-5'-phosphate: Y57 (= Y60), R59 (= R62), S86 (= S89), G87 (= G90), M88 (= M91), Y112 (≠ F115), D186 (= D187), F189 (= F190), S208 (= S209), T210 (= T211), K211 (= K212)

1e5fA Methionine gamma-lyase (mgl) from trichomonas vaginalis
37% identity, 96% coverage: 14:399/401 of query aligns to 7:389/393 of 1e5fA

query
sites
1e5fA

T

T

L

A

G

C

V

I

R

H

A

A

G

N

T

P

M

Q

R

K

S

D

E

Q

F

F

M

G

E

A

H

A

S

I

E

P

A

P

M

I

Y

Y

L

Q

T

T

S

S

S

T

F

F

C

V

F

F

N

D

S

N

A

C

A

Q

E

Q

A

G

A

G

E

N

R

R

F

F

A

A

N

G

S

Q

E

E

S

S

G

G

F

Y

T

I

Y
|
Y

S

T

R
|
R

F

L

T

G

N

N

P

P

T

T

V

V

A

S

M

N

F

L

Q

E

S

G

R

K

L

I

A

A

A

F

L

L

E

E

G

K

A

T

E

E

A

A

C

C

M

V

A

A

T

T

A

S

S

S

G
|
G

M
|
M

S

G

A

A

I

I

M

A

S

A

I

T

V

V

M

L

S

T

A

I

M

L

Q

K

A

A

G

G

D

D

H

H

L

L

V

I

S

S

S

D

R

E

A

C

I

L

F
x
Y

G

G

S

C

T

T

M

H

T

A

L

L

F

F

S

E

N

H

I

A

F

L

A

T

K

K

F

F

G

G

V

I

E

Q

T

V

T

D

F

F

V

I

D

N

G

T

T

A

D

I

L

P

A

G

A

E

W

V

R

K

A

K

A

H

V

M

K

K

P

P

N

N

T

T

K

K

L

I

F

V

F

Y

L

F

E

E

T

T

P

P

S

A

N

N

P

P

L

T

T

L

E

K

V

I

A

I

D

D

I

M

A

E

A

R

V

V

A

C

D

K

I

D

A

A

H

H

N

S

-

Q

A

E

G

G

A

V

L

L

L

V

V

I

V

A

D
|
D

N

N

C

T

F

F

C

C

S

S

P

P

A

M

L

I

Q

T

Q

N

P

P

M

V

K

D

F

F

G

G

A

V

D

D

I

V

V

V

V

V

H

H

S
|
S

A

A

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

T

R

D

V

V

L

V

G

A

G

G

A

L

V

I

L

C

G

G

S

K

H

A

D

D

F

L

I

L

M

Q

G

Q

-

I

K

R

V

M

F

V

P

G

F

I

V

K

R

D

T

I

A

T

G

G

P

S

T

V

L

I

S

S

A

P

F

H

N

D

A

A

W

W

V

L

L

I

L

T

K

R

G

G

M

L

E

S

T

T

L

L

A

N

I

I

R

R

M

M

E

K

R

A

H

E

S

S

Q

E

S

N

A

A

L

M

A

K

L

V

A

A

E

E

F

Y

L

L

E

K

S

S

H

H

P

P

A

A

V

V

A

E

R

K

V

V

Y

Y

H

Y

P

P

A

G

L

F

K

E

S

D

H

H

P

E

Q

G

Y

H

E

D

V

I

A

A

Q

K

R

K

Q

Q

Q

M

S

R

G

M

G

Y

G

G

A

S

I

M

V

I

S

T

F

F

E

I

L

L

K

K

G

S

D

G

T

F

P

-

E

-

Q

-

Q

-

R

-

A

E

N

G

A

A

W

K

R

K

V

L

I

L

D

D

N

N

T

L

R

K

V

L

C

I

S

T

I

L

T

A

G

V

N

S

L

L

G

G

D

G

T

C

R

E

T

S

T

L

V

I

T

Q

H

H

P

P

Y

A

T

S

T

M

T

T

H

H

G

A

R

V

V

V

S

P

P

K

Q

E

A

E

K

R

A

E

A

A

A

A

G

G

I

I

S

T

E

D

G

G

L

M

I

I

R

R

L

L

A

S

V

V

G

G

L

I

E

E

S

D

V

A

A

D

D

E

L

L

K

I

A

A

D

D

L

F

L

K

R

Q

G

G

L

L

active site: R55 (= R62), Y108 (≠ F115), D181 (= D187), K206 (= K212)
binding pyridoxal-5'-phosphate: Y53 (= Y60), R55 (= R62), G83 (= G90), M84 (= M91), Y108 (≠ F115), D181 (= D187), S203 (= S209), K206 (= K212)

1e5eA Methionine gamma-lyase (mgl) from trichomonas vaginalis in complex with propargylglycine
37% identity, 96% coverage: 14:399/401 of query aligns to 7:389/394 of 1e5eA

query
sites
1e5eA

T

T

L

A

G

C

V

I

R

H

A

A

G

N

T

P

M

Q

R

K

S

D

E

Q

F

F

M

G

E

A

H

A

S

I

E

P

A

P

M

I

Y

Y

L

Q

T

T

S

S

S

T

F

F

C

V

F

F

N

D

S

N

A

C

A

Q

E

Q

A

G

A

G

E

N

R

R

F

F

A

A

N

G

S

Q

E

E

S

S

G

G

F

Y

T

I

Y
|
Y

S

T

R
|
R

F

L

T

G

N

N

P

P

T

T

V

V

A

S

M

N

F

L

Q

E

S

G

R

K

L

I

A

A

A

F

L

L

E

E

G

K

A

T

E

E

A

A

C

C

M

V

A

A

T

T

A

S

S

S

G
|
G

M
|
M

S

G

A

A

I

I

M

A

S

A

I

T

V

V

M

L

S

T

A

I

M

L

Q

K

A

A

G

G

D

D

H

H

L

L

V

I

S

S

S

D

R

E

A

C

I

L

F
x
Y

G

G

S

C

T

T

M

H

T

A

L

L

F

F

S

E

N

H

I

A

F

L

A

T

K

K

F

F

G

G

V

I

E

Q

T

V

T

D

F

F

V

I

D

N

G

T

T

A

D

I

L

P

A

G

A

E

W

V

R

K

A

K

A

H

V

M

K

K

P

P

N

N

T

T

K

K

L

I

F

V

F

Y

L

F

E

E

T

T

P

P

S

A

N
|
N

P

P

L

T

T

L

E

K

V

I

A

I

D

D

I

M

A

E

A

R

V

V

A

C

D

K

I

D

A

A

H

H

N

S

-

Q

A

E

G

G

A

V

L

L

L

V

V

I

V

A

D
|
D

N

N

C

T

F

F

C

C

S

S

P

P

A

M

L

I

Q

T

Q

N

P

P

M

V

K

D

F

F

G

G

A

V

D

D

I

V

V

V

V

V

H

H

S
|
S

A

A

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

T

R

D

V

V

L

V

G

A

G

G

A

L

V

I

L

C

G

G

S

K

H

A

D

D

F

L

I

L

M

Q

G

Q

-

I

K

R

V

M

F

V

P

G

F

I

V

K

R

D

T

I

A

T

G

G

P

S

T

V

L

I

S

S

A

P

F

H

N

D

A

A

W

W

V

L

L

I

L

T

K

R

G

G

M

L

E

S

T

T

L

L

A

N

I

I

R

R

M

M

E

K

R

A

H

E

S

S

Q

E

S

N

A

A

L

M

A

K

L

V

A

A

E

E

F

Y

L

L

E

K

S

S

H

H

P

P

A

A

V

V

A

E

R

K

V

V

Y

Y

H

Y

P

P

A

G

L

F

K

E

S

D

H

H

P

E

Q

G

Y

H

E

D

V

I

A

A

Q

K

R

K

Q

Q

Q

M

S

R

G

M

G

Y

G

G

A

S

I

M

V

I

S

T

F

F

E

I

L

L

K

K

G

S

D

G

T

F

P

-

E

-

Q

-

Q

-

R

-

A

E

N

G

A

A

W

K

R

K

V

L

I

L

D

D

N

N

T

L

R

K

V

L

C

I

S

T

I

L

T

A

G

V

N
x
S

L

L

G

G

D

G

T

C

R

E

T

S

T

L

V

I

T

Q

H

H

P

P

Y

A

T

S

T

M

T
|
T

H

H

G

A

R

V

V

V

S

P

P

K

Q

E

A

E

K

R

A

E

A

A

A

A

G

G

I

I

S

T

E

D

G

G

L

M

I

I

R
|
R

L

L

A

S

V

V

G

G

L

I

E

E

S

D

V

A

A

D

D

E

L

L

K

I

A

A

D

D

L

F

L

K

R

Q

G

G

L

L

active site: R55 (= R62), Y108 (≠ F115), D181 (= D187), K206 (= K212)
binding n-(hydroxy{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)norvaline: Y53 (= Y60), <