SitesBLAST
Comparing RR42_RS14380 FitnessBrowser__Cup4G11:RR42_RS14380 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7tcmA Crystal structure of aspartate-semialdehyde dehydrogenase from acinetobacter baumannii in complex with NADP
70% identity, 100% coverage: 1:377/378 of query aligns to 3:370/373 of 7tcmA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G7), R11 (= R9), G12 (= G10), M13 (= M11), V14 (= V12), T38 (= T36), S39 (= S37), C74 (≠ A71), Q75 (= Q72), G76 (= G73), A100 (= A97), S167 (= S164), Q352 (= Q359), A357 (= A364)
5bntC X-ray crystal structure of a aspartate-semialdehyde dehydrogenase bound to NADP from pseudomonas aeruginosa
68% identity, 99% coverage: 3:377/378 of query aligns to 5:370/371 of 5bntC
- active site: C136 (= C134), Q163 (= Q161), R269 (= R276), H276 (= H283)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G7), R11 (= R9), G12 (= G10), M13 (= M11), V14 (= V12), T38 (= T36), S39 (= S37), Q74 (= Q72)
1gl3A Aspartate beta-semialdehyde dehydrogenase in complex with NADP and substrate analogue s-methyl cysteine sulfoxide (see paper)
66% identity, 99% coverage: 3:375/378 of query aligns to 4:366/367 of 1gl3A
- active site: C135 (= C134), Q162 (= Q161), R267 (= R276), H274 (= H283)
- binding cysteine: C135 (= C134), Q162 (= Q161), I230 (= I230), E241 (= E241), R267 (= R276), H274 (= H283)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R10 (= R9), G11 (= G10), M12 (= M11), V13 (= V12), T37 (= T36), S38 (= S37), C72 (≠ A71), Q73 (= Q72), A98 (= A97), C135 (= C134), S165 (= S164), G166 (= G165), G168 (= G167), A169 (= A168), Q350 (= Q359), A355 (= A364)
P0A9Q9 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Escherichia coli (strain K12) (see 2 papers)
66% identity, 99% coverage: 3:375/378 of query aligns to 4:366/367 of P0A9Q9
- RGMV 10:13 (= RGMV 9:12) binding
- TS 37:38 (= TS 36:37) binding
- Q73 (= Q72) binding
- C135 (= C134) active site, Acyl-thioester intermediate; mutation to A: Complete loss of activity.; mutation to S: 99.7% loss of activity.
- SGGGA 165:169 (= SGGGA 164:168) binding
- R173 (= R172) binding
- P193 (= P192) binding
- Q350 (= Q359) binding
3pzrA Crystals structure of aspartate beta-semialdehyde dehydrogenase from vibrio cholerae with NADP and product of s-carbamoyl-l-cysteine (see paper)
64% identity, 99% coverage: 1:376/378 of query aligns to 1:367/370 of 3pzrA
- active site: C134 (= C134), Q161 (= Q161), R267 (= R276), H274 (= H283)
- binding cysteine: C134 (= C134), Q161 (= Q161), G165 (= G165), E240 (= E241), R267 (= R276), H274 (= H283), Q350 (= Q359)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), R9 (= R9), G10 (= G10), M11 (= M11), V12 (= V12), S35 (= S35), T36 (= T36), S37 (= S37)
Q9KQG2 Aspartate-semialdehyde dehydrogenase 1; ASA dehydrogenase 1; ASADH 1; Aspartate-beta-semialdehyde dehydrogenase 1; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
64% identity, 99% coverage: 1:376/378 of query aligns to 1:367/370 of Q9KQG2
- RGMV 9:12 (= RGMV 9:12) binding
- TS 36:37 (= TS 36:37) binding
- Q72 (= Q72) binding
- C134 (= C134) active site, Acyl-thioester intermediate
- SG 164:165 (= SG 164:165) binding
- P192 (= P192) binding
- Q350 (= Q359) binding
4r5mA Crystal structure of vc-aspartate beta-semialdehyde-dehydrogenase with NADP and 4-nitro-2-phosphono-benzoic acid (see paper)
64% identity, 99% coverage: 1:376/378 of query aligns to 1:367/369 of 4r5mA
- active site: C134 (= C134), Q161 (= Q161), R267 (= R276), H274 (= H283)
- binding 4-nitro-2-phosphonobenzoic acid: C71 (≠ A71), G73 (= G73), G74 (= G74), A96 (= A96), A97 (= A97), S98 (= S98), R101 (= R101), K243 (= K244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), R9 (= R9), G10 (= G10), M11 (= M11), V12 (= V12), T36 (= T36), S37 (= S37), Q72 (= Q72), G73 (= G73), G165 (= G165)
1mb4A Crystal structure of aspartate semialdehyde dehydrogenase from vibrio cholerae with NADP and s-methyl-l-cysteine sulfoxide (see paper)
64% identity, 99% coverage: 1:376/378 of query aligns to 1:367/369 of 1mb4A
- active site: C134 (= C134), Q161 (= Q161), R267 (= R276), H274 (= H283)
- binding cysteine: N133 (= N133), C134 (= C134), G165 (= G165), E240 (= E241), R267 (= R276), H274 (= H283)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), R9 (= R9), G10 (= G10), M11 (= M11), V12 (= V12), T36 (= T36), S37 (= S37), C71 (≠ A71), Q72 (= Q72), G73 (= G73), A97 (= A97), S164 (= S164), Q350 (= Q359), L351 (= L360), A355 (= A364)
P44801 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see 2 papers)
61% identity, 99% coverage: 3:376/378 of query aligns to 4:370/371 of P44801
- RGMV 10:13 (= RGMV 9:12) binding
- TS 37:38 (= TS 36:37) binding
- Q74 (= Q72) binding
- R103 (= R101) binding ; mutation to K: 2-fold increase in affinity for ASA, 23-fold decrease in affinity for phosphate, and 275-fold decrease in activity.; mutation to L: 7-fold increase in affinity for ASA, 150-fold decrease in affinity for phosphate, and 1400-fold decrease in activity.
- C136 (= C134) active site, Acyl-thioester intermediate
- S166 (= S164) binding
- E243 (= E241) mutation to D: No change in affinity for ASA and 82-fold decrease in activity.
- K246 (= K244) binding ; mutation to R: 2-fold increase in affinity for ASA, nearly no change in affinity for phosphate, and 30-fold decrease in activity.
- R270 (= R276) mutation to K: 2-fold decrease in affinity for ASA and 825-fold decrease in activity.
- H277 (= H283) active site, Proton acceptor
- Q353 (= Q359) binding
1pquA Crystal structure of the h277n mutant of aspartate semialdehyde dehydrogenase from haemophilus influenzae bound with NADP, s-methyl cysteine sulfoxide and cacodylate (see paper)
61% identity, 99% coverage: 3:376/378 of query aligns to 4:370/371 of 1pquA
- active site: C136 (= C134), Q163 (= Q161), R270 (= R276), N277 (≠ H283)
- binding cysteine: C136 (= C134), Q163 (= Q161), G167 (= G165), E243 (= E241), R270 (= R276)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), R10 (= R9), G11 (= G10), M12 (= M11), V13 (= V12), T36 (≠ S35), T37 (= T36), S38 (= S37), C73 (≠ A71), Q74 (= Q72), A99 (= A97), S166 (= S164), L354 (= L360), A358 (= A364)
1tb4A Crystal structure of aspartate-semialdehyde dehydrogenase from haemophilus influenzae with a bound periodate (see paper)
60% identity, 99% coverage: 3:376/378 of query aligns to 4:356/357 of 1tb4A
1ta4A Crystal structure of aspartate-semialdehyde dehydrogenase from haemophilus influenzae with a bound arsenate (see paper)
60% identity, 99% coverage: 3:376/378 of query aligns to 4:356/357 of 1ta4A
1nx6A Crystal structure of aspartate semialdehyde dehydrogenase from haemophilus influenzae as a tetrahedral hemithiocetal reaction intermediate with phosphate at 2.15 a (see paper)
60% identity, 99% coverage: 3:376/378 of query aligns to 4:356/357 of 1nx6A
1pqpA Crystal structure of the c136s mutant of aspartate semialdehyde dehydrogenase from haemophilus influenzae bound with aspartate semialdehyde and phosphate (see paper)
60% identity, 99% coverage: 3:376/378 of query aligns to 4:356/357 of 1pqpA
- active site: S122 (≠ C134), Q149 (= Q161), R256 (= R276), H263 (= H283)
- binding l-homoserine: S122 (≠ C134), Q149 (= Q161), G153 (= G165), R256 (= R276), H263 (= H283), Q339 (= Q359)
- binding phosphate ion: R89 (= R101), N121 (= N133), S122 (≠ C134), K232 (= K244)
Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
25% identity, 96% coverage: 3:365/378 of query aligns to 7:330/346 of Q04797
- S98 (= S98) modified: Phosphoserine
- Y146 (≠ D150) modified: Phosphotyrosine
4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
25% identity, 96% coverage: 3:365/378 of query aligns to 4:330/357 of 4r54A
- binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G73), S73 (≠ G74), T94 (≠ A97), S95 (= S98), R98 (= R101), K222 (= K244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), T10 (≠ R9), G11 (= G10), A12 (≠ M11), V13 (= V12), A35 (≠ S35), S36 (≠ T36), R38 (≠ N38), S39 (≠ A39), T56 (≠ A56), S70 (≠ A71), A71 (≠ Q72), G72 (= G73), T75 (≠ Y76), N93 (≠ A96), T94 (≠ A97), N126 (= N133), C127 (= C134), G160 (= G167), G328 (= G363)
4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
25% identity, 96% coverage: 3:365/378 of query aligns to 4:330/357 of 4r41A
- binding 4-nitro-2-phosphonobenzoic acid: S70 (≠ A71), G72 (= G73), S73 (≠ G74), N93 (≠ A96), T94 (≠ A97), S95 (= S98), R98 (= R101), K222 (= K244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), T10 (≠ R9), G11 (= G10), A12 (≠ M11), A35 (≠ S35), S36 (≠ T36), R38 (≠ N38), S39 (≠ A39), T56 (≠ A56), A71 (≠ Q72), G160 (= G167), M161 (≠ A168), G162 (≠ Q169)
4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
25% identity, 96% coverage: 3:365/378 of query aligns to 4:330/357 of 4r3nA
- active site: C127 (= C134), Q154 (= Q161), R244 (= R276), H251 (= H283)
- binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G74), T94 (≠ A97), S95 (= S98), R98 (= R101), N126 (= N133), K222 (= K244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), T10 (≠ R9), G11 (= G10), A12 (≠ M11), V13 (= V12), A35 (≠ S35), S36 (≠ T36), S39 (≠ A39), T56 (≠ A56), S70 (≠ A71), A71 (≠ Q72), G72 (= G73), N93 (≠ A96), T94 (≠ A97), N126 (= N133), C127 (= C134), G160 (= G167), M161 (≠ A168), G328 (= G363)
3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
25% identity, 96% coverage: 3:365/378 of query aligns to 4:330/357 of 3q1lA
3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
25% identity, 96% coverage: 3:365/378 of query aligns to 4:330/357 of 3pwsA
- binding (2R)-2-aminohexanedioic acid: R98 (= R101), N126 (= N133), G158 (= G165), I208 (= I230), E219 (= E241), K222 (= K244), R244 (= R276)
- binding adenosine-2'-5'-diphosphate: G8 (= G7), T10 (≠ R9), G11 (= G10), A12 (≠ M11), A35 (≠ S35), S36 (≠ T36), R38 (≠ N38), S39 (≠ A39), T56 (≠ A56), A71 (≠ Q72), T75 (≠ Y76), G160 (= G167), M161 (≠ A168)
Query Sequence
>RR42_RS14380 FitnessBrowser__Cup4G11:RR42_RS14380
MIVGLVGWRGMVGSVLMQRMQEERDFDHIEPVFFSTSNAGGQAPAMAKNETKLKDANDIE
ALKKCDVVLTAQGGDYTNEIFPQLRAAGWKGYWIDAASSLRMKDDAIIVLDPVNLGVIKD
ALAKGVKNFIGGNCTVSCMMMGLGGLFQHDLIDWMTSMTYQAASGGGAQHMRELLTQFGT
LNASVKSLLDNPASAILEIDRTILATQHGLSADETKQFGVPLAGNLIPWIDKDLGNGVSK
EEWKAGAETNKILGRGEGFLGATGAAPIAVDGLCVRIGAMRCHSQALTIKLRRDVPLDEI
EAMLAENNQWAKVVPNTREASMTDLTPAAVTGTLTIPVGRLRKMQMGGEYLSAFTVGDQL
LWGAAEPLRRMLRILIEA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory