SitesBLAST
Comparing RR42_RS17660 FitnessBrowser__Cup4G11:RR42_RS17660 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
52% identity, 100% coverage: 2:246/246 of query aligns to 4:244/244 of 4nbuB
- active site: G18 (= G16), N111 (= N113), S139 (= S141), Q149 (= Q151), Y152 (= Y154), K156 (= K158)
- binding acetoacetyl-coenzyme a: T91 (= T93), D93 (= D95), K98 (= K100), S139 (= S141), T141 (≠ V143), N146 (= N148), V147 (≠ F149), G148 (= G150), Q149 (= Q151), Y152 (= Y154), F184 (= F186), M189 (≠ I191), K200 (≠ G202)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), A16 (= A14), N17 (≠ A15), G18 (= G16), I19 (= I17), D38 (= D36), F39 (≠ V37), N40 (≠ Q38), V59 (= V61), D60 (= D62), V61 (= V63), N87 (= N89), A88 (= A90), G89 (= G91), I90 (= I92), V110 (= V112), T137 (≠ A139), S138 (= S140), S139 (= S141), Y152 (= Y154), K156 (= K158), P182 (= P184), G183 (= G185), F184 (= F186), T185 (≠ V187), T187 (= T189), A188 (≠ E190), M189 (≠ I191), V190 (≠ L192)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
52% identity, 100% coverage: 2:246/246 of query aligns to 2:239/239 of 4nbtA
- active site: G16 (= G16), S132 (= S141), Y145 (= Y154), K149 (= K158)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), K15 (≠ A15), G16 (= G16), L17 (≠ I17), D36 (= D36), L37 (≠ V37), L52 (≠ V61), N53 (≠ D62), V54 (= V63), N80 (= N89), A81 (= A90), G82 (= G91), I83 (= I92), V103 (= V112), I130 (≠ A139), S131 (= S140), S132 (= S141), Y145 (= Y154), K149 (= K158), P177 (= P184), G178 (= G185), Y179 (≠ F186), I180 (≠ V187), T182 (= T189)
4nbwA Crystal structure of fabg from plesiocystis pacifica (see paper)
54% identity, 97% coverage: 6:244/246 of query aligns to 2:249/253 of 4nbwA
- active site: G12 (= G16), S146 (= S141), Y159 (= Y154), K163 (= K158)
- binding nicotinamide-adenine-dinucleotide: G8 (= G12), A10 (= A14), N11 (≠ A15), G12 (= G16), I13 (= I17), D32 (= D36), L33 (≠ V37), V57 (= V61), D58 (= D62), V59 (= V63), N85 (= N89), A86 (= A90), G87 (= G91), I88 (= I92), V117 (= V112), A144 (= A139), S145 (= S140), S146 (= S141), Y159 (= Y154), K163 (= K158), P189 (= P184), G190 (= G185), I192 (≠ V187), T194 (= T189)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
48% identity, 100% coverage: 1:246/246 of query aligns to 4:247/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G12), A17 (= A14), R18 (≠ A15), G19 (= G16), I20 (= I17), D39 (= D36), R40 (≠ Q38), C63 (≠ V61), N64 (≠ D62), I65 (≠ V63), N91 (= N89), A92 (= A90), G93 (= G91), I94 (= I92), V114 (= V112), I141 (≠ A139), S143 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), F187 (= F186), I188 (≠ V187), T190 (= T189), M192 (≠ I191), T193 (≠ L192)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
46% identity, 100% coverage: 1:246/246 of query aligns to 1:247/247 of 4jroC
- active site: G16 (= G16), S142 (= S141), Q152 (= Q151), Y155 (= Y154), K159 (= K158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (≠ A15), G16 (= G16), I17 (= I17), N35 (vs. gap), Y36 (≠ C35), N37 (≠ D36), G38 (≠ V37), S39 (≠ Q38), A62 (≠ V61), N63 (≠ D62), V64 (= V63), N90 (= N89), A91 (= A90), G92 (= G91), I93 (= I92), I113 (≠ V112), A141 (≠ S140), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), I188 (≠ V187), T190 (= T189)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
44% identity, 100% coverage: 1:246/246 of query aligns to 1:246/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
44% identity, 98% coverage: 6:246/246 of query aligns to 2:239/239 of 3sj7A
- active site: G12 (= G16), S138 (= S141), Q148 (= Q151), Y151 (= Y154), K155 (= K158)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (≠ A14), R11 (≠ A15), G12 (= G16), I13 (= I17), N31 (≠ C35), Y32 (≠ D36), A33 (≠ V37), G34 (vs. gap), S35 (≠ Q38), A58 (≠ V61), N59 (≠ D62), V60 (= V63), N86 (= N89), A87 (= A90), G88 (= G91), I89 (= I92), T109 (≠ V112), L136 (≠ A139), S137 (= S140), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), F183 (= F186), I184 (≠ V187)
4cqlI Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD (see paper)
42% identity, 99% coverage: 2:244/246 of query aligns to 5:249/251 of 4cqlI
- active site: G19 (= G16), S146 (= S141), Y159 (= Y154), K163 (= K158)
- binding nicotinamide-adenine-dinucleotide: G15 (= G12), G17 (≠ A14), S18 (≠ A15), G19 (= G16), I20 (= I17), D39 (= D36), L40 (≠ V37), A64 (≠ V61), D65 (= D62), V66 (= V63), C93 (≠ N89), A94 (= A90), G95 (= G91), I96 (= I92), V116 (= V112), I144 (≠ A139), S146 (= S141), Y159 (= Y154), K163 (= K158), P189 (= P184), G190 (= G185), F191 (= F186), I192 (≠ V187), T194 (= T189), P195 (≠ E190), M196 (≠ I191), T197 (≠ L192)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
43% identity, 100% coverage: 1:246/246 of query aligns to 1:245/248 of Q9KJF1
- M1 (= M1) modified: Initiator methionine, Removed
- S15 (≠ A15) binding
- D36 (= D36) binding
- D62 (= D62) binding
- I63 (≠ V63) binding
- N89 (= N89) binding
- Y153 (= Y154) binding
- K157 (= K158) binding
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
43% identity, 99% coverage: 3:246/246 of query aligns to 2:244/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), S14 (≠ A15), M16 (≠ I17), D35 (= D36), I36 (≠ V37), A60 (≠ V61), D61 (= D62), I62 (≠ V63), N88 (= N89), A89 (= A90), G90 (= G91), V111 (= V112), N112 (= N113), I138 (≠ A139), A139 (≠ S140), S140 (= S141), Y152 (= Y154), K156 (= K158), P182 (= P184), G183 (= G185), I185 (≠ V187), M189 (≠ I191)
4cqmA Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD and NADP (see paper)
42% identity, 99% coverage: 2:244/246 of query aligns to 3:246/248 of 4cqmA
- active site: G17 (= G16), S143 (= S141), Y156 (= Y154), K160 (= K158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G12), G15 (≠ A14), S16 (≠ A15), G17 (= G16), I18 (= I17), D37 (= D36), L38 (≠ V37), A61 (≠ V61), D62 (= D62), V63 (= V63), C90 (≠ N89), A91 (= A90), G92 (= G91), I93 (= I92), V113 (= V112), I141 (≠ A139), S142 (= S140), S143 (= S141), Y156 (= Y154), K160 (= K158), P186 (= P184), G187 (= G185), F188 (= F186), I189 (≠ V187), T191 (= T189), P192 (≠ E190), M193 (≠ I191), T194 (≠ L192)
Q92506 (3R)-3-hydroxyacyl-CoA dehydrogenase; 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; HSD17B8; 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit; KAR alpha subunit; 3-oxoacyl-[acyl-carrier-protein] reductase; Estradiol 17-beta-dehydrogenase 8; Protein Ke6; Ke6; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17-beta-dehydrogenase 8; EC 1.1.1.n12; EC 1.1.1.62; EC 1.1.1.239 from Homo sapiens (Human) (see 2 papers)
41% identity, 99% coverage: 2:244/246 of query aligns to 8:259/261 of Q92506
- 15:23 (vs. 9:17, 56% identical) binding
- D42 (= D36) mutation to A: Reduced NADH-dependent reductase activity with acetoacetyl-CoA. Reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Increases NADPH-dependent reductase activities. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- DL 42:43 (≠ DV 36:37) binding
- ADV 74:76 (≠ VDV 61:63) binding
- R148 (≠ K133) mutation to E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- V158 (= V143) to L: in a breast cancer sample; somatic mutation
- Y169 (= Y154) mutation to A: Strongly reduced NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- YAASK 169:173 (= YAASK 154:158) binding
- K173 (= K158) mutation to A: Abolishes NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Slightly decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- R189 (≠ P174) mutation to E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- IAT 202:204 (≠ VAT 187:189) binding
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
45% identity, 99% coverage: 3:246/246 of query aligns to 4:246/247 of P73574
- A14 (= A13) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ F149) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K158) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (= F186) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (vs. gap) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
4dmmB 3-oxoacyl-[acyl-carrier-protein] reductase from synechococcus elongatus pcc 7942 in complex with NADP
43% identity, 100% coverage: 1:246/246 of query aligns to 1:239/240 of 4dmmB
- active site: G16 (= G16), S142 (= S141), Q152 (= Q151), Y155 (= Y154), K159 (= K158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (≠ A15), G16 (= G16), I17 (= I17), N35 (≠ C35), Y36 (≠ D36), A37 (≠ V37), S38 (≠ Q38), S39 (≠ E39), A62 (≠ V61), D63 (= D62), V64 (= V63), N90 (= N89), A91 (= A90), G92 (= G91), I93 (= I92), L113 (≠ V112), I140 (≠ A139), A141 (≠ S140), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), F187 (= F186), I188 (≠ V187), T190 (= T189), D191 (≠ E190), M192 (vs. gap), T193 (vs. gap)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
39% identity, 100% coverage: 1:246/246 of query aligns to 1:243/244 of 6t77A
- active site: G16 (= G16), S138 (= S141), Y151 (= Y154)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (≠ A15), A36 (≠ D36), T37 (≠ V37), L58 (≠ V61), N59 (≠ D62), V60 (= V63), A87 (= A90), G88 (= G91), I89 (= I92), T109 (≠ V112)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
40% identity, 100% coverage: 1:246/246 of query aligns to 4:246/247 of 3op4A