SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS19695 FitnessBrowser__Cup4G11:RR42_RS19695 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
54% identity, 70% coverage: 28:103/109 of query aligns to 3:78/81 of 1a56A

query
sites
1a56A

E

D

I

L

A

A

N

K

K

K

N

N

A
x
N

C
|
C

M

I

G

A

C
|
C

H
|
H

Q

Q

V

V

D

E

K

T

K

K

L

V

V
|
V

G
|
G

P
|
P

A

A

Y

L

K

K

D

D

V

I

A

A

K

K

Y

Y

K

A

G

D

D

K

K

D

N

D

A

A

L

A

A

T

T

Y

L

L

T

A

K

G

K
|
K

V
x
I

K

K

G

G

G
x
S

S

S

G

G

V

V

W
|
W

G

G

P
x
Q

V
x
I

P

P

M
|
M

P

P

A

P

N
|
N

A
x
V

A
x
N

L

V

S

S

D

D

A

A

D

D

L

A

K

K

T

A

V

L

V

A

E

D

W

W

V

I

L

L

binding heme c: N9 (≠ A34), C10 (= C35), C13 (= C38), H14 (= H39), V21 (= V46), G22 (= G47), P23 (= P48), K45 (= K70), I46 (≠ V71), S50 (≠ G75), W54 (= W79), Q56 (≠ P81), I57 (≠ V82), M59 (= M84), N62 (= N87), V63 (≠ A88), N64 (≠ A89)

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
45% identity, 95% coverage: 1:104/109 of query aligns to 1:102/104 of P00099

query
sites
P00099

M
|
M

K
|
K

Q
x
P

F
x
Y

V
x
A

I
x
L

A
x
L

T
x
S

L
|
L

M
x
L

L
x
A

A
x
T

C
x
G

A
x
T

A
x
L

S
x
L

A
|
A

N
x
Q

A
x
G

A
|
A

V
x
W

D

-

A

-

A
|
A

K
x
E

A
x
D

Q
x
P

E
|
E

I
x
V

A
x
L

N
x
F

K
|
K

N
|
N

-
x
K

A
x
G

C
|
C

M
x
V

G
x
A

C
|
C

H
|
H

Q
x
A

V
x
I

D
|
D

K
x
T

K
|
K

L
x
M

V
|
V

G
|
G

P
|
P

A
|
A

Y
|
Y

K
|
K

D
|
D

V
|
V

A
|
A

K
|
K

Y
x
F

K
x
A

G
|
G

D
x
Q

K
x
A

N
x
G

A
|
A

L
x
E

A
|
A

T
x
E

L
|
L

T
x
A

K
x
Q

K
x
R

V
x
I

K
|
K

G
x
N

G
|
G

G
x
S

S
x
Q

G
|
G

V
|
V

W
|
W

G
|
G

P
|
P

V
x
I

P
|
P

M
|
M

P
|
P

A
x
P

N
|
N

A

-

A
|
A

L
x
V

S
|
S

D
|
D

A
x
D

D
x
E

L
x
A

K
x
Q

T
|
T

V
x
L

V
x
A

E
x
K

W
|
W

V
|
V

L
|
L

A
x
S

1:22 (vs. 1:24, 25% identical) signal peptide
H38 (= H39) binding axial binding residue
M83 (= M84) binding axial binding residue

Sites not aligning to the query:

23:104 modified: mature protein, Cytochrome c-551

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
50% identity, 72% coverage: 27:104/109 of query aligns to 2:78/80 of 5xecC

query
sites
5xecC

Q

E

E

Q

I

L

A

A

N

K

K

Q

N

K

A

G

C
|
C

M

M

G

A

C
|
C

H
|
H

Q

D

V

L

D

K

K

A

K

K

L

M

V

V

G

G

P
|
P

A

A

Y
|
Y

K

K

D

D

V

V

A

A

K

K

Y

F

K

A

G

G

D

Q

K

A

N

G

A

A

L

E

A

A

T

E

L
|
L

T

A

K

Q

K
x
R

V

I

K

K

G

N

G

G

G

S

S
x
Q

G

G

V
|
V

W
|
W

G
|
G

P

P

V
x
I

P

P

M
|
M

P

P

A

P

N
|
N

A

-

A

A

L

V

S

S

D

D

A

D

D

E

L

A

K

Q

T

T

V

L

V

A

E

K

W

W

V

V

L

L

A

S

binding heme c: C10 (= C35), C13 (= C38), H14 (= H39), P23 (= P48), Y25 (= Y50), L42 (= L67), R45 (≠ K70), Q51 (≠ S76), V53 (= V78), W54 (= W79), G55 (= G80), I57 (≠ V82), M59 (= M84), N62 (= N87)

2d0sA Crystal structure of the cytochrome c552 from moderate thermophilic bacterium, hydrogenophilus thermoluteolus (see paper)
52% identity, 71% coverage: 27:103/109 of query aligns to 2:76/79 of 2d0sA

query
sites
2d0sA

Q

E

E

A

I

L

A

A

N

K

K

A

N

K

A

G

C
|
C

M

M

G

A

C
|
C

H
|
H

Q

A

V

I

D

D

K

K

L

L

V

V

G

G

P
|
P

A

S

Y

Y

K

K

D

D

V

V

A

A

A

K

K

K

Y
|
Y

K

-

G

-

D

T

K

E

N

A

A

D

L

V

A

P

T

K

L

L

T

V

K

E

K
|
K

V
|
V

K

K

G

K

G

G

S
x
A

G
|
G

V
|
V

W
|
W

G
|
G

P

P

V
|
V

P

P

M
|
M

P
|
P

A

P

N
x
H

A

P

A

Q

L

V

S

A

D

E

A

A

D

D

L

I

K

E

T

K

V

I

V

V

E

R

W

W

V

V

L

L

binding heme c: C10 (= C35), C13 (= C38), H14 (= H39), P23 (= P48), Y32 (= Y57), K43 (= K70), V44 (= V71), A49 (≠ S76), G50 (= G77), V51 (= V78), W52 (= W79), G53 (= G80), V55 (= V82), M57 (= M84), P58 (= P85), H60 (≠ N87)

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
51% identity, 70% coverage: 29:104/109 of query aligns to 6:80/82 of 2exvA

query
sites
2exvA

I

L

A

A

N

K

K

N

N

K

A

G

C
|
C

M

V

G

A

C
|
C

H
|
H

Q

A

V

I

D

D

K

T

K

K

L

M

V

V

G
|
G

P
|
P

A

A

Y

Y

K

K

D

D

V

V

A

A

K

K

Y

F

K

A

G

G

D

Q

K

A

N

G

A

A

L

E

A

A

T

E

L

L

T

A

K

Q

K
x
R

V
x
I

K

K

G

N

G

G

G
x
S

S
x
Q

G
|
G

V
|
V

W
|
W

G
|
G

P

P

V
x
I

P
|
P

M
|
M

P

P

A

P

N
|
N

A

-

A

A

L

V

S

S

D

D

A

D

D

E

L

A

K

Q

T

T

V

L

V

A

E

K

W

W

V

V

L

L

A

S

binding heme c: C12 (= C35), C15 (= C38), H16 (= H39), G24 (= G47), P25 (= P48), R47 (≠ K70), I48 (≠ V71), S52 (≠ G75), Q53 (≠ S76), G54 (= G77), V55 (= V78), W56 (= W79), G57 (= G80), I59 (≠ V82), P60 (= P83), M61 (= M84), N64 (= N87)

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
53% identity, 71% coverage: 28:104/109 of query aligns to 4:80/82 of 351cA

query
sites
351cA

E

E

I

V

A

L

N

F

K

K

N

N

-

K

A

G

C
|
C

M

V

G

A

C
|
C

H
|
H

Q

A

V

I

D

D

K

T

K

K

L

M

V

V

G
|
G

P
|
P

A

A

Y
|
Y

K

K

D

D

V

V

A

A

K

K

Y

F

K

A

G

G

D

Q

K

A

N

G

A

A

L

E

A

A

T

E

L
|
L

T

A

K

Q

K
x
R

V
x
I

K

K

G

N

G

G

G

S

S
x
Q

G
|
G

V
|
V

W
|
W

G
|
G

P

P

V
x
I

P
|
P

M
|
M

P

P

A

P

N

N

A

-

A

A

L

V

S

S

D

D

A

D

D

E

L

A

K

Q

T

T

V

L

V

A

E

K

W

W

V

V

L

L

A

S

binding protoporphyrin ix containing fe: C12 (= C35), C15 (= C38), H16 (= H39), G24 (= G47), P25 (= P48), Y27 (= Y50), L44 (= L67), R47 (≠ K70), I48 (≠ V71), Q53 (≠ S76), G54 (= G77), V55 (= V78), W56 (= W79), G57 (= G80), I59 (≠ V82), P60 (= P83), M61 (= M84)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
51% identity, 71% coverage: 28:104/109 of query aligns to 4:80/82 of 5xecA

query
sites
5xecA

E

E

I

V

A

L

N

F

K

K

N

N

-

K

A

G

C
|
C

M

V

G

A

C
|
C

H
|
H

Q

A

V

I

D

D

K

T

K

K

L

K

V

V

G
|
G

P
|
P

A

A

Y
|
Y

K

A

D

D

V

V

A

A

A

K

K

K

Y
|
Y

K

A

G

G

D

R

K

K

N

D

A

A

L

V

A

D

T
x
Y

L

L

T

A

K

G

K

K

V
x
I

K

K

G

K

G

G

S
|
S

G
|
G

V
|
V

W
|
W

G
|
G

P

S

V
|
V

P

P

M
|
M

P

P

A

P

N
x
Q

A

-

A

N

L

V

S

T

D

D

A

A

D

E

L

A

K

K

T

Q

V

L

V

A

E

Q

W

W

V
x
I

L

L

A

S

binding heme c: C12 (= C35), C15 (= C38), H16 (= H39), G24 (= G47), P25 (= P48), Y27 (= Y50), Y34 (= Y57), Y43 (≠ T66), I48 (≠ V71), S53 (= S76), G54 (= G77), V55 (= V78), W56 (= W79), G57 (= G80), V59 (= V82), M61 (= M84), Q64 (≠ N87), I78 (≠ V102)

1corA Investigation of the solution conformation of cytochromE C-551 from pseudomonas stutzeri (see paper)
49% identity, 64% coverage: 35:104/109 of query aligns to 12:80/82 of 1corA

query
sites
1corA

C
|
C

M

A

G

A

C
|
C

H
|
H

Q

S

V

I

D

D

K

A

K

K

L

L

V
|
V

G
|
G

P
|
P

A

A

Y

F

K

K

D

E

V

V

A

A

K

K

Y

Y

K

A

G

G

D

Q

K

D

N

G

A

A

L

A

A

D

T

L

L
|
L

T

A

K

G

K
x
H

V
x
I

K

K

G

N

G

G

G
x
S

S
x
Q

G
|
G

V
|
V

W
|
W

G
|
G

P

P

V
x
I

P

P

M
|
M

P

P

A

P

N
|
N

A

-

A

P

L

V

S

T

D

E

A

E

D

E

L

A

K

K

T

I

V

L

V

A

E

E

W

W

V

I

L

L

A

S

binding heme c: C12 (= C35), C15 (= C38), H16 (= H39), V23 (= V46), G24 (= G47), P25 (= P48), L44 (= L67), H47 (≠ K70), I48 (≠ V71), S52 (≠ G75), Q53 (≠ S76), G54 (= G77), V55 (= V78), W56 (= W79), G57 (= G80), I59 (≠ V82), M61 (= M84), N64 (= N87)

Sites not aligning to the query:

binding heme c: 10

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
47% identity, 72% coverage: 27:104/109 of query aligns to 2:81/83 of 5aurA

query
sites
5aurA

Q

E

E

Q

I

L

A

A

N

K

K

Q

N

K

A

G

C
|
C

M

M

G

A

C
|
C

H
|
H

Q

D

V

L

D

K

-

A

-

G

K

G

K

K

L

K

V

V

G

G

P
|
P

A

A

Y
|
Y

K

A

D

D

V

V

A

A

A

K

K

K

Y
|
Y

K

A

G

G

D

R

K

K

N

D

A

A

L

V

A

D

T
x
Y

L

L

T

A

K

G

K

K

V
x
I

K

K

G

K

G

G

S
|
S

G
|
G

V
|
V

W
|
W

G
|
G

P

S

V
|
V

P
|
P

M
|
M

P

P

A

P

N
x
Q

A

-

A

N

L

V

S

T

D

D

A

A

D

E

L

A

K

K

T

Q

V

L

V

A

E

Q

W

W

V

I

L

L

A

S

binding heme c: C10 (= C35), C13 (= C38), H14 (= H39), P26 (= P48), Y28 (= Y50), Y35 (= Y57), Y44 (≠ T66), I49 (≠ V71), S54 (= S76), G55 (= G77), V56 (= V78), W57 (= W79), G58 (= G80), V60 (= V82), P61 (= P83), M62 (= M84), Q65 (≠ N87)

6kq1A Crystal structure of cytochrome c551 from pseudomonas sp. Strain mt-1.
49% identity, 64% coverage: 35:104/109 of query aligns to 12:80/82 of 6kq1A

query
sites
6kq1A

C
|
C

M

A

G

A

C
|
C

H
|
H

Q

S

V

I

D

D

K

A

K

K

L

M

V

V

G
|
G

P
|
P

A

A

Y

L

K

K

D

E

V
|
V

A

A

K

K

Y
|
Y

K

A

G

G

D

Q

K

E

N

G

A

A

L

A

A

D

T

L

L
|
L

T

A

K

G

K
x
H

V
x
I

K

K

G

N

G

G

G

T

S
x
Q

G
|
G

V
x
N

W
|
W

G
|
G

P

P

V
x
I

P

P

M
|
M

P

P

A

P

N
|
N

A

-

A

P

L

V

S

T

D

E

A

E

D
x
E

L

A

K

K

T

T

V

L

V

A

E

E

W

W

V

V

L

L

A

S

binding heme c: C12 (= C35), C15 (= C38), H16 (= H39), G24 (= G47), P25 (= P48), V30 (= V53), Y34 (= Y57), L44 (= L67), H47 (≠ K70), I48 (≠ V71), Q53 (≠ S76), G54 (= G77), N55 (≠ V78), W56 (= W79), G57 (= G80), I59 (≠ V82), M61 (= M84), N64 (= N87)
binding zinc ion: E70 (≠ D94)

Sites not aligning to the query:

binding zinc ion: 2, 4, 10

1cchA The solution conformation of cytochromE C-551 from p.Stutzeri zobell determined by nmr+ (see paper)
50% identity, 64% coverage: 35:104/109 of query aligns to 12:80/82 of 1cchA

query
sites
1cchA

C
|
C

M

A

G

A

C
|
C

H
|
H

Q

S

V

V

D

D

K

T

K

K

L

M

V

V

G

G

P

P

A

A

Y

L

K

K

D

E

V

V

A

A

K

K

Y

N

K

A

G

G

D

V

K

E

N

G

A

A

L

A

A

D

T

T

L
|
L

T

A

K

L

K
x
H

V
x
I

K

K

G

N

G

G

G
x
S

S
x
Q

G

G

V
|
V

W
|
W

G
|
G

P

P

V
x
I

P

P

M
|
M

P
|
P

A

P

N
|
N

A

-

A

P

L

V

S

T

D

E

A

E

D

E

L

A

K

K

T

I

V

L

V

A

E

E

W

W

V

V

L

L

A

S

binding heme c: C12 (= C35), C15 (= C38), H16 (= H39), L44 (= L67), H47 (≠ K70), I48 (≠ V71), S52 (≠ G75), Q53 (≠ S76), V55 (= V78), W56 (= W79), G57 (= G80), I59 (≠ V82), M61 (= M84), P62 (= P85), N64 (= N87)

4xxlA Crystal structure of class 1 cytochrome mtod from sideroxydans lithotrophicus es-1 (see paper)
36% identity, 78% coverage: 20:104/109 of query aligns to 1:89/92 of 4xxlA

query
sites
4xxlA

A

A

V

V

D

D

A

V

A

D

K

A

A

A

Q

K

E

S

I

L

A

A

N

R

K

E

N

N

A

N

C
|
C

M

F

G

K

C
|
C

H
|
H

Q

G

V

V

D

D

K

K

K

E

L

K

V

D

G
|
G

P
|
P

A

S

Y
|
Y

K

K

D

K

V

V

A

A

A

E

K
|
K

Y
|
Y

K

R

G

G

D

K

K

A

N

D

A

A

L

E

A

A

T

K

L

L

T

I

K

H

K
x
H

V

V

K

T

G

S

G

G

G

E

S

K

G
x
A

V
x
K

W

F

-

P

-

D

-

G

-

H

G

E

P

E

V

E

P
x
H

M

K

P

N

A

I

N

N

A

G

A

K

L

A

S

S

D

P

A

E

D

A

L

I

K

K

T

N

V

L

V

V

E

D

W

W

V

I

L

L

A

S

binding heme c: C16 (= C35), C19 (= C38), H20 (= H39), G28 (= G47), P29 (= P48), Y31 (= Y50), K37 (= K56), Y38 (= Y57), H51 (≠ K70), A58 (≠ G77), K59 (≠ V78), H68 (≠ P83)

Query Sequence

>RR42_RS19695 FitnessBrowser__Cup4G11:RR42_RS19695
MKQFVIATLMLACAASANAAVDAAKAQEIANKNACMGCHQVDKKLVGPAYKDVAAKYKGD
KNALATLTKKVKGGGSGVWGPVPMPANAALSDADLKTVVEWVLAGAPAK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory