SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS19775 FitnessBrowser__Cup4G11:RR42_RS19775 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

4jxrB Crystal structure of a gnat superfamily phosphinothricin acetyltransferase (pat) from sinorhizobium meliloti in complex with accoa
47% identity, 84% coverage: 30:206/211 of query aligns to 2:175/185 of 4jxrB

query
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4jxrB

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binding acetyl coenzyme *a: A29 (= A57), D86 (= D113), S87 (= S114), I88 (≠ V115), Y89 (= Y116), L90 (≠ I117), P92 (≠ E119), E93 (≠ R120), R95 (= R122), G96 (= G123), G98 (= G125), I99 (≠ L126), G100 (= G127), H127 (≠ G158), S129 (≠ G160), S130 (= S161), L133 (= L164), H134 (= H165)

5dwnA Crystal structure of phosphinothricin n-acetyltransferase from brucella ovis in complex with acetylcoa
49% identity, 82% coverage: 31:204/211 of query aligns to 4:175/181 of 5dwnA

query
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binding acetyl coenzyme *a: D86 (= D113), S87 (= S114), I88 (≠ V115), Y89 (= Y116), I90 (= I117), K95 (≠ R122), G96 (= G123), Q97 (= Q124), G98 (= G125), I99 (≠ L126), G100 (= G127), K101 (≠ L128), A121 (= A148), V122 (= V149), I123 (≠ V150), G131 (= G160), S132 (= S161), K134 (≠ A163), L135 (= L164), H136 (= H165), S138 (≠ R167)

2j8mA Structure of p. Aeruginosa acetyltransferase pa4866 (see paper)
40% identity, 79% coverage: 30:196/211 of query aligns to 1:164/171 of 2j8mA

query
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binding azide ion: W73 (≠ Y101), R74 (= R102), N126 (≠ E157), S129 (= S161), F159 (≠ L191)

A6VCX3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.- from Pseudomonas aeruginosa (strain PA7) (see paper)
40% identity, 79% coverage: 30:196/211 of query aligns to 2:165/172 of A6VCX3

query
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RPF 75:77 (≠ RTR 102:104) binding
EHS 85:87 (≠ EDS 112:114) binding

2j8rA Structure of p. Aeruginosa acetyltransferase pa4866 solved in complex with l-methionine sulfoximine (see paper)
40% identity, 78% coverage: 32:196/211 of query aligns to 2:163/170 of 2j8rA

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binding azide ion: Y18 (= Y48), R39 (≠ I69), W42 (≠ R72), Y66 (= Y95), S85 (= S114), Y87 (= Y116), R110 (≠ E139), L163 (= L196)
binding (2s)-2-amino-4-(methylsulfonimidoyl)butanoic acid: A27 (= A57), I28 (≠ S58), W29 (≠ F59), R73 (= R102), F75 (≠ R104), F78 (≠ Y107), E83 (= E112), H84 (≠ D113), S85 (= S114), A120 (≠ C152)

Sites not aligning to the query:

binding azide ion: 165

4jwpA Crystal structure of ribosomal-protein-alanine n-acetyltransferase from brucella melitensis in complex with acetyl coa
39% identity, 78% coverage: 33:196/211 of query aligns to 6:165/165 of 4jwpA

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binding acetyl coenzyme *a: A30 (= A57), L86 (≠ D113), V88 (= V115), Y89 (= Y116), V90 (≠ I117), R95 (= R122), G96 (= G123), G97 (≠ Q124), G98 (= G125), I99 (≠ L126), G100 (= G127), R101 (≠ L128), G122 (≠ C152), I123 (≠ T153), N127 (≠ G158), A129 (≠ G160), S130 (= S161), A132 (= A163), L133 (= L164), H134 (= H165), S136 (≠ R167)
binding calcium ion: E110 (≠ R137), E113 (≠ S140)

6m7gA Crystal structure of arsn, n-acetyltransferase with substrate phosphinothricin from pseudomonas putida kt2440 (see paper)
40% identity, 79% coverage: 32:198/211 of query aligns to 3:164/176 of 6m7gA

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F

W

A

A

I

V

E

D

D

V

S

T

V

V

Y

Y

I

V

D

A

E

E

R

G

Y

Q

R

R

G

R

Q

S

G

G

L

I

G

A

L

R

A

Q

L

L

L

Y

A

D

A

V

L

L

V

L

S

P

R

V

C

L

E

K

S

R

G

L

P

G

W

Y

R

R

Q

S

M

A

V

Y

A

A

V

G

V

I

A

A

C

L

T

P

A

-

S

-

G

-

E

-

G

N

A

E

G

G

S

S

L

V

A

G

L

L

H

H

E

E

R

R

V

L

G

G

F

F

R

Q

T

H

I

I

G

G

R

T

L

F

Q

P

S

Q

V

V

G

G

F

F

K

K

H

L

G

D

Q

A

W

W

I

H

D

D

T

V

V

G

L

Y

M

W

Q

R

R

F

P

D

L

F

G

G

D

D

binding phosphinothricin: R74 (= R104), Y77 (= Y107)

5wphA Crystal structure of arsn, n-acetyltransferase with substrate ast from pseudomonas putida kt2440 (see paper)
40% identity, 79% coverage: 32:198/211 of query aligns to 3:164/179 of 5wphA

query
sites
5wphA

S

D

I

I

R

R

D

V

A

A

T

R

A

P

A

E

D

D

I

A

P

E

A

E

I

I

Q

Q

A

I

I

I

Y

Y

A

A

H

P

H

I

V

V

R

L

H

N

G

T

R

A

A
x
I

S
|
S

F
|
F

E

E

V

A

E

V

P

P

G

S

V

V

D

E

D

Q

I

M

R

R

L

E

R

R

H

-

A

I

E

S

V

T

R

T

R

L

Q

Q

G

T

L

Y

P

P

Y

Y

L

L

V

V

A

A

E

V

R

R

G

E

G

G

E

R

V

V

L

V

G

G

Y

Y

A

A

Y

Y

A

A

S

S

A

Q

Y

H

R
|
R

T
x
A

R
|
R

S

A

A

A

Y
|
Y

R

R

F

W

A

A

I

V

E

D

D

V

S

T

V

V

Y

Y

I

V

D

A

E

E

R

G

Y

Q

R

R

G

R

Q

S

G

G

L

I

G

A

L

R

A

Q

L

L

L

Y

A

D

A

V

L

L

V

L

S

P

R

V

C

L

E

K

S

R

G

L

P

G

W

Y

R

R

Q

S

M

A

V

Y

A

A

V

G

V

I

A

A

C

L

T

P

A

-

S

-

G

-

E

-

G

N

A

E

G

G

S

S

L

V

A

G

L

L

H

H

E

E

R

R

V

L

G

G

F

F

R

Q

T

H

I

I

G

G

R

T

L

F

Q

P

S

Q

V

V

G

G

F

F

K

K

H

L

G

D

Q

A

W

W

I

H

D

D

T

V

V

G

L

Y

M

W

Q

R

R

F

P

D

L

F

G

G

D

D

binding (2S)-2-amino-4-[hydroxy(methyl)arsoryl]butanoic acid: I28 (≠ A57), S29 (= S58), F30 (= F59), R72 (= R102), A73 (≠ T103), R74 (= R104), Y77 (= Y107)

5t7eD Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with coenzyme a and l-phosphinothricin (see paper)
35% identity, 77% coverage: 32:193/211 of query aligns to 3:159/175 of 5t7eD

query
sites
5t7eD

S

D

I

I

R

R

D

R

A

A

T

T

A

E

A

A

D

D

I

M

P

P

A

A

I

V

Q

C

A

T

I

I

Y

V

A

N

H

H

H
x
Y

V

I

R

E

H

T

G

S

R

T

A
x
V

S
x
N

F
|
F

-

R

-

T

-

E

-

P

E

Q

E

E

V

P

E

Q

P

E

G

W

V

T

D

D

-

L

I

V

R

R

L

L

R

R

H

-

A

-

E

-

V

-

R

-

Q

E

G

R

L

Y

P

P

Y

W

L

L

V

V

A

A

E

E

R

V

G

D

G

G

E

E

V

V

L

A

G

G

Y

I

A

A

Y

Y

A

A

S

G

A

P

Y

W

R
x
K

T

A

R
|
R

S

N

A

A

Y
|
Y

R

D

F

W

A

T

I

A

E

E

D

S

S
x
T

V
|
V

Y
|
Y

I
x
V

D

S

E

P

R

R

Y

H

R
x
Q

G
x
R

Q
x
T

G
|
G

L
|
L

G
|
G

L
x
S

A

T

L

L

L

Y

A

T

A

H

L

L

V

L

S

K

R

S

C

L

E

E

S

A

G

Q

P

G

W

F

R

K

Q

S

M

V

V

V

A

A

V
|
V

V
x
I

A
x
G

C

L

T

P

A
x
N

S

D

G

-

E

-

G

-

A

-

G
x
P

S
|
S

L

V

A
x
R

L
x
M

H

H

E

E

R

A

V

L

G

G

F

Y

R

A

T

P

I

R

G

G

R

M

L

L

Q

R

S

A

V

A

G

G

F

F

K

K

H

H

G

G

Q

N

W

W

I

H

D

D

T

V

V

G

L

F

M

W

Q

Q

binding coenzyme a: Y22 (≠ H51), V28 (≠ A57), V85 (= V115), Y86 (= Y116), V87 (≠ I117), Q92 (≠ R122), R93 (≠ G123), T94 (≠ Q124), G95 (= G125), L96 (= L126), G97 (= G127), S98 (≠ L128), I120 (≠ V150), N124 (≠ A154), P126 (≠ G160), S127 (= S161), R129 (≠ A163), M130 (≠ L164)
binding phosphinothricin: V28 (≠ A57), N29 (≠ S58), F30 (= F59), K72 (≠ R102), R74 (= R104), Y77 (= Y107), T84 (≠ S114), Y86 (= Y116), V119 (= V149), G121 (≠ A151)

5t7dA Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with acetyl coenzyme a (see paper)
35% identity, 77% coverage: 32:193/211 of query aligns to 2:158/173 of 5t7dA

query
sites
5t7dA

S

D

I

I

R

R

D

R

A

A

T

T

A

E

A

A

D

D

I

M

P

P

A

A

I

V

Q

C

A

T

I

I

Y

V

A

N

H

H

H
x
Y

V

I

R

E

H

T

G

S

R

T

A
x
V

S

N

F

F

-

R

-

T

-

E

-

P

E

Q

E

E

V

P

E

Q

P

E

G

W

V

T

D

D

-

L

I

V

R

R

L

L

R

R

H

-

A

-

E

-

V

-

R

-

Q

E

G

R

L

Y

P

P

Y

W

L

L

V

V

A

A

E

E

R

V

G

D

G

G

E

E

V

V

L

A

G

G

Y

I

A

A

Y

Y

A

A

S

G

A

P

Y

W

R

K

T

A

R

R

S

N

A

A

Y

Y

R

D

F

W

A

T

I

A

E

E

D

S

S
x
T

V
|
V

Y
|
Y

I
x
V

D

S

E

P

R

R

Y

H

R
x
Q

G
x
R

Q
x
T

G
|
G

L
|
L

G
|
G

L
x
S

A

T

L

L

L

Y

A

T

A

H

L

L

V

L

S

K

R

S

C

L

E

E

S

A

G

Q

P

G

W

F

R

K

Q

S

M

V

V

V

A

A

V
|
V

V
x
I

A

G

C

L

T

P

A
x
N

S

D

G

-

E

-

G

-

A

-

G
x
P

S
|
S

L

V

A
x
R

L
x
M

H
|
H

E

E

R

A

V

L

G

G

F

Y

R

A

T

P

I

R

G

G

R

M

L

L

Q

R

S

A

V

A

G

G

F

F

K

K

H

H

G

G

Q

N

W

W

I

H

D

D

T

V

V

G

L

F

M

W

Q

Q

binding acetyl coenzyme *a: Y21 (≠ H51), V27 (≠ A57), T83 (≠ S114), V84 (= V115), Y85 (= Y116), V86 (≠ I117), Q91 (≠ R122), R92 (≠ G123), T93 (≠ Q124), G94 (= G125), L95 (= L126), G96 (= G127), S97 (≠ L128), V118 (= V149), I119 (≠ V150), N123 (≠ A154), P125 (≠ G160), S126 (= S161), R128 (≠ A163), M129 (≠ L164), H130 (= H165)

3dr8A Structure of ynca, a putative acetyltransferase from salmonella typhimurium with its cofactor acetyl-coa
35% identity, 81% coverage: 29:198/211 of query aligns to 1:166/173 of 3dr8A

query
sites
3dr8A

A

S

S

N

P

M

S

T

I

I

R

R

D

F

A

A

T

D

A

K

A

A

D

D

I

C

P

A

A

A

I

I

Q

T

A

E

I

I

Y

Y

A

N

H

H

H

A

V

V

R

L

H

H

G

T

R

A

A
|
A

S

I

F

W

E

N

E

D

V

R

E

T

P

V

G

D

V

T

D

D

D

N

I

-

R

R

L

L

R

A

H

W

A

Y

E

E

V

A

R

R

R

Q

Q

L

-

L

G

G

L

Y

P

P

Y

V

L

L

V

V

A

S

E

E

R

E

G

N

G

G

E

V

V

V

L

T

G

G

Y

Y

A

A

Y

S

A

F

S

G

A

D

Y

W

R

R

T

S

R

F

S

D

A

G

Y

F

R

R

F

Y

A

T

I

V

E

E

D
x
H

S
|
S

V
|
V

Y
|
Y

I
x
V

D

H

E

P

R

A

Y

H

R
x
Q

G
|
G

Q
x
K

G
|
G

L
|
L

G
|
G

L
x
R

A

K

L

L

A

S

A

R

L

L

V

I

S

D

R

E

C

A

E

R

S

R

G

C

P

G

W

K

R

H

Q

V

M

M

V

V

A
|
A

V
x
G

V
x
I

A

-

C

-

T

-

A

-

S

E

G

S

E

Q

G
x
N

A
|
A

G
x
A

S
|
S

L

I

A
x
R

L
|
L

H
|
H

E

H

R
x
S

V

L

G

G

F

F

R

T

T

V

I

T

G

A

R

Q

L

M

Q

P

S

Q

V

V

G

G

F

V

K

K

H

F

G

G

Q

R

W

W

I

L

D

D

T

L

V

T

L

F

M

M

Q

Q

R

L

P

Q

L

L

G

D

D

E

binding acetyl coenzyme *a: A29 (= A57), H85 (≠ D113), S86 (= S114), V87 (= V115), Y88 (= Y116), V89 (≠ I117), Q94 (≠ R122), G95 (= G123), K96 (≠ Q124), G97 (= G125), L98 (= L126), G99 (= G127), R100 (≠ L128), A120 (= A148), G121 (≠ V149), I122 (≠ V150), N126 (≠ G158), A127 (= A159), A128 (≠ G160), S129 (= S161), R131 (≠ A163), L132 (= L164), H133 (= H165), S135 (≠ R167)

Q8ZPD3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; L-amino acid N-acyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.-; EC 2.3.1.- from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
35% identity, 79% coverage: 32:198/211 of query aligns to 2:164/171 of Q8ZPD3

query
sites
Q8ZPD3

S

T

I

I

R

R

D

F

A

A

T

D

A

K

A

A

D

D

I

C

P

A

A

A

I

I

Q

T

A

E

I

I

Y

Y

A

N

H

H

H

A

V

V

R

L

H

H

G

T

R

A

A

A

S

I

F

W

E

N

E

D

V

R

E

T

P

V

G

D

V

T

D

D

D

N

I

-

R

R

L

L

R

A

H

W

A

Y

E

E

V

A

R

R

R

Q

Q

L

-

L

G

G

L

Y

P

P

Y

V

L

L

V

V

A

S

E

E

R

E

G

N

G

G

E

V

V

V

L

T

G

G

Y

Y

A

A

Y

S

A

F

S

G

A

D

Y

W

R

R

T

S

R

F

S

D

A

G

Y

F

R

R

F

Y

A

T

I

V

E
|
E

D

H

S

S

V
|
V

Y
|
Y

I
x
V

D

H

E

P

R

A

Y

H

R

Q

G
|
G

Q
x
K

G
|
G

L
|
L

G
|
G

L
x
R

A

K

L

L

A

S

A

R

L

L

V

I

S

D

R

E

C

A

E

R

S

R

G

C

P

G

W

K

R

H

Q

V

M

M

V

V

A

A

V

G

V

I

A

-

C

-

T

-

A

-

S

E

G

S

E

Q

G
x
N

A

A

G

A

S

S

L

I

A

R

L

L

H

H

E

H

R
x
S

V

L

G

G

F

F

R

T

T

V

I

T

G

A

R

Q

L

M

Q

P

S

Q

V

V

G

G

F

V

K

K

H

F

G

G

Q

R

W

W

I

L

D

D

T

L

V

T

L

F

M

M

Q

Q

R

L

P

Q

L

L

G

D

D

E

E82 (= E112) mutation to Q: Loss of acetyltransferase activity.
VYV 85:87 (≠ VYI 115:117) binding
GKGLGR 93:98 (≠ GQGLGL 123:128) binding
N124 (≠ G158) binding
S133 (≠ R167) binding

4mbuA Crystal structure of n-acetyltransferase from staphylococcus aureus mu50 (see paper)
33% identity, 80% coverage: 29:196/211 of query aligns to 1:164/165 of 4mbuA

query
sites
4mbuA

A

S

S

N

P

A

S

M

I

I

R

R

D

C

A

A

T

K

A

K

A

E

D

D

I

L

P

N

A

A

I

I

Q

L

A

A

I

I

Y

Y

A

N

H

D

H

A

V

I

R

I

H

N

G

T

R

T

A

A

S

V

F

Y

E

T

E

-

V

Y

E

K

P

P

G

Q

V

T

D

I

D

D

I

E

R

R

L

I

R

A

H

W

A

F

E

E

V

T

R

K

-

Q

R

R

Q

N

G

H

L

E

P

P

Y

I

L

F

V

V

A

F

E

E

R

E

G

N

G

G

E

S

V

V

L

L

G

G

Y

F

A

A

Y

T

A

F

S

G

A

S

Y

F

R

R

T

P

R

W

S

P

A

A

Y

Y

R

Q

F

Y

A

T

I

I

E

E

D

H

S

S

V

I

Y

Y

I

V

D

D

E

A

R

S

Y

A

R

R

G

G

Q

K

G

G

L

I

G

A

L

S

A

Q

L

L

A

Q

A

R

L

L

V

I

S

V

R

E

C

A

E

K

S

A

G

K

P

G

W

Y

R

R

Q

T

M

L

V

V

A

A

V

-

A

G

C

I

T

D

A

A

S

S

G

N

E

E

G

-

A

-

G

A

S

S

L

I

A

K

L

L

H

H

E
x
Q

R

K

V

F

G

N

F

F

R
x
K

T
x
H

I

A

G

G

R

T

L

L

Q

T

S

N

V

V

G

G

F

Y

K

K

H

F

G

D

Q

Y

W

W

I

L

D

D

T

L

V

A

L

F

M
x
Y

Q

E

R

L

P

D

L

L

binding phosphate ion: Q134 (≠ E166), K139 (≠ R171), H140 (≠ T172), Y160 (≠ M192)

2jlmF Structure of a putative acetyltransferase (aciad1637) from acinetobacter baylyi adp1 (see paper)
34% identity, 58% coverage: 74:196/211 of query aligns to 52:171/180 of 2jlmF

query
sites
2jlmF

A

A

E

T

V

K

R

R

R

Q

Q

N

G

N

L

F

P

P

Y

I

L

I

V

G

A

A

-

V

E

N

R

E

G

V

G

G

E

Q

V

L

L

L

G

G

Y

F

A

A

Y

S

A

W

S

G

A

S

Y

F

R

R

T

A

R

F

S

P

A

A

Y

Y

R

K

F

Y

A

T

I

V

E

E

D

H

S

S

V

V

Y
|
Y

I
|
I

D

H

E

K

R

D

Y

Y

R
|
R

G

G

Q

L

G

G

L

L

G

S

L

K

A

H

L

L

L

M

A

N

A

E

L

L

V

I

S

K

R

R

C

A

E

V

S

E

G

S

P

E

W

V

R

H

Q

V

M

M

V

V

A

G

V

C

V
x
I

A
x
D

C

A

T

T

A

-

S

-

G

-

E

-

G
x
N

A

V

G

A

S

S

L

I

A

Q

L

L

H

H

E

Q

R

K

V

L

G

G

F

F

R

I

T

H

I

S

G

G

R

T

L

I

Q

Q

S

Q

V

A

G

G

F

F

K

K

H

F

G

G

Q

R

W

W

I

L

D

D

T

A

V

A

L

F

M

Y

Q

Q

R

L

P

T

L

L

binding azide ion: Y95 (= Y116), I96 (= I117), R101 (= R122), I129 (≠ V150), D130 (≠ A151), N133 (≠ G158)

Sites not aligning to the query:

binding azide ion: 34, 35

Query Sequence

>RR42_RS19775 FitnessBrowser__Cup4G11:RR42_RS19775
MKAPLATLSAACATPMAVANATAAVQACASPSIRDATAADIPAIQAIYAHHVRHGRASFE
EVEPGVDDIRLRHAEVRRQGLPYLVAERGGEVLGYAYASAYRTRSAYRFAIEDSVYIDER
YRGQGLGLALLAALVSRCESGPWRQMVAVVACTASGEGAGSLALHERVGFRTIGRLQSVG
FKHGQWIDTVLMQRPLGDGDETPPVERAAKP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory