SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS21080 FitnessBrowser__Cup4G11:RR42_RS21080 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
44% identity, 93% coverage: 12:169/170 of query aligns to 5:163/170 of Q58667

query
sites
Q58667

G

G

R

R

I

A

W

H

R

K

F

F

G

G

D

D

N

D

V

V

D

D

T

T

D

D

Q

A

M

I

A

I

P

P

G

G

T

P

H

Y

M

L

K
x
R

G
x
T

G

T

V

D

-

P

E

Y

E

E

I

L

A

A

R

S

H

H

C

C

L

M

A

A

G

G

L

I

R

D

P

E

E

N

F

F

P

P

A

K

V

V

R

K

P

E

G

G

D

D

L

V

L

I

V

V

A

A

G

G

R

E

N

N

F

F

G

G

V

C

G

G

S

S

R

R

E

E

Q

Q

A

A

P

V

Q

I

A

A

L

I

R

K

H

Y

L

C

G

G

L

I

A

K

A

A

V

V

I

I

A

A

P

K

S

S

F

F

A

A

G

R

L

I

F

F

Y

Y

R

R

N

N

A

A

I

I

N

N

L

V

G

G

L

L

-

I

P

P

V

I

L

I

V

A

C

N

H

T

D

D

A

E

T

I

R

K

L

-

V

-

D

D

G

G

A

D

A

I

A

V

R

E

L

I

E

D

L

L

G

D

Q

K

A

E

C

E

L

I

V

V

L

I

P

T

D

N

G

K

S

N

R

K

-

T

V

I

A

K

C

C

E

E

P

T

I

P

P

K

E

G

F

L

L

E

H

R

A

E

L

I

L

L

Q

A

A

A

G

G

L

L

V

V

P

N

H

Y

L

L

K

K

A

K

R

R

L

K

A

L

A

I

Q

Q

R26 (≠ K33) mutation R->K,V: Allows the recognition of substrates of IPMI enzymes since it becomes able to efficiently catalyze citraconate hydration and 3-isopropylmalate dehydration. Largely decreases substrate affinity for cis-homo(1-3)-aconitate.
T27 (≠ G34) mutation to A: Largely decreases substrate affinity for cis-homo(1-3)-aconitate while slightly increases activity on these substrates, and also decreases substrate affinity for maleate. Gains the ability to hydrate citraconate, an IPMI substrate.

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
44% identity, 93% coverage: 12:169/170 of query aligns to 5:163/167 of 2pkpA

query
sites
2pkpA

G

G

R

R

I

A

W

H

R

K

F

F

G

G

D

D

N
x
D

V

V

D

D

T

T

D

D

Q

A

M

I

A

I

P

P

G

G

T

P

H

Y

M

L

K

R

G

T

V

D

-

P

E

Y

E

E

I

L

A

A

R

S

H

H

C

C

L

M

A

A

G

G

L

I

R

D

P

E

E

N

F

F

P

P

A

K

V

V

R

K

P

E

G

G

D

D

L

V

L

I

V

V

A

A

G

G

R

E

N

N

F

F

G

G

V
x
C

G

G

S

S

R

R

E

E

Q

Q

A

A

P

V

Q

I

A

A

L

I

R

K

H

Y

L

C

G

G

L

I

A

K

A

A

V

V

I

I

A

A

P

K

S

S

F

F

A

A

G

R

L

I

F

F

Y

Y

R

R

N

N

A

A

I

I

N

N

L

V

G

G

L

L

-

I

P

P

V

I

L

I

V

A

C

N

H

T

D

D

A

E

T

I

R

K

L

-

V

-

D

D

G

G

A

D

A

I

A

V

R

E

L

I

E

D

L

L

G

D

Q

K

A

E

C

E

L

I

V

V

L

I

P

T

D

N

G

K

S

N

R

K

-

T

V

I

A

K

C

C

E

E

P

T

I

P

P

K

E

G

F

L

L

E

H

R

A

E

L

I

L

L

Q

A

A

A

G

G

L

L

V

V

P

N

H

Y

L

L

K

K

A

K

R

R

L

K

A

L

A

I

Q

Q

binding zinc ion: D13 (≠ N20), C63 (≠ V69)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
40% identity, 52% coverage: 20:108/170 of query aligns to 554:655/758 of O14289

query
sites
O14289

N

N

V

V

D

D

T

T

D

D

Q

K

M

I

A

I

P

P

G

K

T

Q

H

F

M

L

K

K

G

T

-

I

-

K

-

R

-

T

G

G

V

L

E

G

E

Q

I

F

A

A

-

F

-

Y

-

E

-

I

R

R

H

Y

C

D

L

A

A

D

G

G

L

K

R

E

-

I

P

P

E

D

F

F

P

V

A

L

A

N

V

R

R

E

P

P

-

Y

-

R

-

H

G

A

D

T

L

V

L

L

V

V

A

A

G

H

R

D

N

N

F

F

G

G

V

C

G

G

S

S

R

R

E

E

Q

H

A

A

P

P

Q

W

A

A

L

L

R

N

H

D

L

F

G

G

L

I

A

R

A

V

V

I

I

I

A

A

P

P

S

S

F

F

A

A

G

D

L

I

F

F

Y

F

R

N

N

N

A

C

I

F

N

K

L

N

G

G

-

M

L

L

P

P

V

I

Sites not aligning to the query:

486 modified: Phosphoserine
488 modified: Phosphoserine

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
42% identity, 51% coverage: 20:106/170 of query aligns to 18:110/198 of P9WK95

query
sites
P9WK95

N

N

V

V

D

D

T

T

D

D

Q

Q

M

I

A

I

P

P

G

A

T

V

H

F

M

L

K

K

-

R

-

V

-

T

-

R

G

T

G

G

V

F

E

E

E

D

I

-

A

-

R

-

H

G

C

L

L

F

A

A

G

G

L

W

R

R

-

S

-

D

P

P

E

A

F

F

P

V

A

L

A

N

V

L

R

S

P

P

-

F

-

D

-

R

G

G

D

S

L

V

L

L

V

V

A

A

G

G

R

P

N

D

F

F

G

G

V

T

G

G

S

S

R

R

E

E

Q

H

A

A

P

V

Q

W

A

A

L

L

R

M

H

D

L

Y

G

G

L

F

A

R

A

V

V

V

I

I

A

S

P

S

S

R

F

F

A

G

G

D

L

I

F

F

Y

R

R

G

N

N

A

A

I

G

N

K

L

A

G

G

L

L

Sites not aligning to the query:

154 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Query Sequence

>RR42_RS21080 FitnessBrowser__Cup4G11:RR42_RS21080
MSPAPIPPLPGGRIWRFGDNVDTDQMAPGTHMKGGVEEIARHCLAGLRPEFPAAVRPGDL
LVAGRNFGVGSSREQAPQALRHLGLAAVIAPSFAGLFYRNAINLGLPVLVCHDATRLVDG
AAARLELGQACLVLPDGSRVACEPIPEFLHALLQAGGLVPHLKARLAAQR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory