SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS21770 RR42_RS21770 dihydrolipoamide dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P14218 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex; EC 1.8.1.4 from Pseudomonas fluorescens (see 2 papers)
41% identity, 100% coverage: 3:466/466 of query aligns to 2:472/478 of P14218

query
sites
P14218

N

S

S

Q

S

K

F

F

D

D

L

V

I

V

V

V

V

I

G

G

G

A

G

G

P

P

G

G

Y

Y

V

V

A

A

I

I

R

R

A

A

T

A

Q

Q

L

L

G

G

M

L

K

K

T

T

A

A

L

C

V

I

E
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E

K
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K

-
x
Y

-
x
I

-
x
G

-
x
K

-
x
E

-
x
G

-
x
K

A
x
V

H
x
A

L
|
L

G
|
G

I
x
T

C
|
C

L

L

N

N

W

V

G

G

C
|
C

I

I

P

P

T

S

K
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K

A

A

L

L

R

D

S

S

A

S

D

Y

V

K

L

Y

R

H

L

E

V

A

K

K

D

E

A

A

-

F

A

K

A

V

Y

H

G

G

V

I

H

E

A

A

A

K

P

G

P

V

T

T

A

I

D

D

L

V

P

P

A

A

M

M

V

V

A

A

R

R

S

K

R

A

A

N

V

I

A

V

A

K

Q

N

L

L

N

T

K

G

G

G

V

I

A

A

H

T

L

L

M

F

K

K

K

A

N

N

G

G

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V

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T

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L

F

N

E

G

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H

H

G
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G

R

K

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L

A

L

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A

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N

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E

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A

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D

D

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A

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A

L

D

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K

D

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I

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G

A

A

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I

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G

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A

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A

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D

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C

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A

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L

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G

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N

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K

G

Q

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V

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T

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T

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D

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A

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-

S

N

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D

D

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K

V

L

L

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A

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N

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D

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G

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D

V

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G

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P

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A
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A

H

H

K

K

A

A

S

S

H

E

E

E

G

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I

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C

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E

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R

I

I

A

A

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G

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-

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H

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M

D

N

A

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Y

Y

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T

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H

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P

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E

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A

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V

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G

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E

A

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Q

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A

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E

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E

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N

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T

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P

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F

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M

T

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F

E

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T

T

T

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E

A

A

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D

L

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M

G

A

M

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V

L

F

P

S

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H

P

P

T

T

M

L

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S

E

E

A

A

M

L

H

H

E

E

A

A

V

A

L

L

A

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A

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Y

N

G

G

R

H

A

A

L

I

H

H

I

I

34:49 (vs. 35:43, 38% identical) binding
C49 (= C43) modified: Disulfide link with 54, Redox-active
C54 (= C48) modified: Disulfide link with 49, Redox-active
K58 (= K52) binding
G122 (= G115) binding
D319 (= D312) binding
A327 (= A320) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5u8uD Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa (see paper)
41% identity, 100% coverage: 3:466/466 of query aligns to 4:474/477 of 5u8uD

query
sites
5u8uD

N

S

S

Q

S

K

F

F

D

D

L

V

I

V

V

V

V
x
I

G
|
G

G
x
A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

I

I

R

R

A

A

T

A

Q

Q

L

L

G

G

M

L

K

K

T

T

A

A

L

C

V
x
I

E
|
E

K
|
K

-
x
Y

-

I

-

G

-

K

-

E

-

G

-

K

A

V

H

A

L
|
L

G

G

G
|
G

I
x
T

C
|
C

L

L

N

N

W
x
V

G
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G

C
|
C

I

I

P

P

T
x
S

K
|
K

A

A

L

L

R

D

S

S

A

S

D

Y

V

K

L

Y

R

H

L

E

V

A

K

K

D

E

A

A

-

F

A

K

A

V

Y

H

G

G

V

I

H

E

A

A

A

K

P
x
G

P
x
V

T

T

A

I

D

D

L

V

P

P

A

A

M

M

V

V

A

A

R

R

S

K

R

A

A

N

V

I

A

V

A

K

Q

N

L

L

N

T

K

G

G

G

V

I

A

A

H

T

L

L

M

F

K

K

K

A

N

N

G

G

V

V

T

T

V

S

L

F

N

E

G
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G

H
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H

G
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G

R

K

L

L

A

L

G

A

K

N

G

K

K

Q

L

V

E

E

V

V

T

T

A

G

T

L

D

D

G

G

K

K

T

T

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I

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V

L

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E

S

A

A

E

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N

I

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A

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x
S

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G

A
x
S

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A

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L

I

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P

A

P

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A

P

P

A

L

D

T

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D

K

D

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I

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x
S

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T

D

G

A

A

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L

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F

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V

A

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V

P

P

K

K

R

K

M

L

L

G

I

V

V

I

G

G

A

A

G

G

A
x
V

I
|
I

G

G

I

L

E
|
E

F

L

A

G

S

S

F

V

Y

W

R

A

A

R

V

L

G

G

A

A

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E

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T

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V

V

L

E

E

M

A

A

L

Q

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R

K

I

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L

P

P

V

A

E

A

D

D

A

E

D

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I

I

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A

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K

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K

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x
R

V

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D

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F

A

A

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G

D
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D

V

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A

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x
M

L
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L

A
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A

H
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H

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A
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S
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S

H

E

E

E

G

G

V

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C

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A

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V

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G

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E

A

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Q

T

A

L

R

K

E

A

R

E

G

G

H

V

P

E

V

V

K

N

V

V

G

G

K

T

F

F

P

P

F

F

A

A

G

A

N

S

G

G

K

R

A

A

I

M

A

A

M

A

G

N

S

D

T

T

A

T

G

G

L

L

V

V

K

K

V

V

V

I

F

A

D

D

A

A

G

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T

G

D

E

R

L

V

L

L

G

G

A

V

H

H

M

V

V

I

G

G

D

P

E

S

V

A

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A

E

E

M

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G

Q

Y

G

A

A

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I

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L

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E

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E

A

A

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D

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A

M

V

V

L
x
F

P

S

H
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H

P
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P

T

T

M

L

S

S

E
|
E

A

A

M

L

H

H

E

E

A

A

V

A

L

L

A

A

A

V

Y

N

G

G

R

H

A

A

L

I

H

H

I

I

active site: P16 (= P15), L47 (= L39), C51 (= C43), C56 (= C48), S59 (≠ T51), G85 (≠ P76), V86 (≠ P77), V193 (≠ A184), E197 (= E188), S333 (= S324), F451 (≠ L443), H453 (= H445), E458 (= E450)
binding flavin-adenine dinucleotide: I12 (≠ V11), G13 (= G12), A14 (≠ G13), G15 (= G14), P16 (= P15), G17 (= G16), I35 (≠ V34), E36 (= E35), K37 (= K36), Y38 (vs. gap), G49 (= G41), T50 (≠ I42), C51 (= C43), V54 (≠ W46), G55 (= G47), C56 (= C48), S59 (≠ T51), K60 (= K52), G122 (= G113), H123 (= H114), G124 (= G115), A152 (= A143), S153 (≠ T144), G154 (= G145), S155 (≠ A146), S173 (≠ Y164), I194 (= I185), R281 (≠ I273), G320 (= G311), D321 (= D312), M327 (≠ W318), L328 (= L319), A329 (= A320), H330 (= H321), A332 (= A323), Y360 (= Y352), H453 (= H445), P454 (= P446)

Sites not aligning to the query:

active site: 476, 477

5u8wA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to nadh (see paper)
41% identity, 100% coverage: 3:466/466 of query aligns to 1:471/473 of 5u8wA

query
sites
5u8wA

N

S

S

Q

S

K

F

F

D

D

L

V

I

V

V

V

V
x
I

G
|
G

G
x
A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

I

I

R

R

A

A

T

A

Q

Q

L

L

G

G

M

L

K

K

T

T

A

A

L

C

V
x
I

E
|
E

K
|
K

-
x
Y

-

I

-

G

-

K

-

E

-

G

-

K

A

V

H

A

L
|
L

G

G

G
|
G

I
x
T

C
|
C

L

L

N

N

W
x
V

G
|
G

C
|
C

I

I

P

P

T
x
S

K
|
K

A

A

L

L

R

D

S

S

A

S

D

Y

V

K

L

Y

R

H

L

E

V

A

K

K

D

E

A

A

-

F

A

K

A

V

Y

H

G

G

V

I

H

E

A

A

A

K

P
x
G

P
x
V

T

T

A

I

D

D

L

V

P

P

A

A

M

M

V

V

A

A

R

R

S

K

R

A

A

N

V

I

A

V

A

K

Q

N

L

L

N

T

K

G

G

G

V

I

A

A

H

T

L

L

M

F

K

K

K

A

N

N

G

G

V

V

T

T

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S

L

F

N

E

G
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G

H
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H

G
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G

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K

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L

A

L

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A

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G

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E

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A

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M

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L

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I

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G

A

A

G
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G

A
x
V

I
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I

G

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I

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E
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E

F

L

A

G

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S

F

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Y

W

R

A

A

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V

L

G

G

A

A

E

E

V

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T

V

V

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x
L

E
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E

M
x
A

A
x
L

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K

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F

L

L

P

P

V

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E

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D

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A

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D

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C

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A

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L

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G

T

A

G

R

I

V

Q

T

S

A

A

S

E

E

R

V

K

K

G

K

Q

K

G

Q

W

V

S

T

I

V

E

T

L

F

Q

T

G

D

G

A

K

-

S

N

G

G

G

E

A

Q

R

K

V

E

D

T

V

F

D

D

V

K

V

L

L

I

V

V

A
|
A

A
x
V

G
|
G

I
x
R

V

R

G

P

N

V

V

T

E

T

D

D

L

L

G

L

L

A

E

A

R

D

T

S

A

G

V

V

K

T

V

L

-

D

E

E

K

R

T

G

H

F

I

I

V

Y

T

V

D

D

G

D

F

H

G

C

R

K

T

T

D

S

E

V

P

P

G

G

V

V

Y

F

A

A

I

I

G
|
G

D
|
D

V

V

A

V

G

R

P

G

P

A

W
x
M

L
|
L

A
|
A

H
|
H

K

K

A
|
A

S
|
S

H

E

E

E

G

G

V

V

I

M

C

V

V

A

E

E

R

R

I

I

A

A

G

G

L

-

H

H

P

K

H

A

A

Q

L

M

D

N

A

Y

T

D

R

L

I

I

P

P

A

S

C
x
V

T

I

Y
|
Y

S

T

H

H

P

P

Q

E

V

I

A

A

S

W

V

V

G

G

L

K

T

T

E

E

A

Q

Q

T

A

L

R

K

E

A

R

E

G

G

H

V

P

E

V

V

K

N

V

V

G

G

K

T

F

F

P

P

F

F

A

A

G

A

N

S

G

G

K

R

A

A

I

M

A

A

M

A

G

N

S

D

T

T

A

T

G

G

L

L

V

V

K

K

V

V

V

I

F

A

D

D

A

A

G

K

S

T

G

D

E

R

L

V

L

L

G

G

A

V

H

H

M

V

V

I

G

G

D

P

E

S

V

A

T

A

E

E

M

L

I

V

Q

Q

G

Q

Y

G

A

A

I

I

A

G

Q

M

T

E

L

F

E

G

T

T

T

S

E

A

A

E

D

D

L

L

M

G

A

M

V

V

L
x
F

P

S

H
|
H

P
|
P

T

T

M

L

S

S

E
|
E

A

A

M

L

H

H

E

E

A

A

V

A

L

L

A

A

A

V

Y

N

G

G

R

H

A

A

L

I

H

H

I

I

active site: P13 (= P15), L44 (= L39), C48 (= C43), C53 (= C48), S56 (≠ T51), G82 (≠ P76), V83 (≠ P77), V190 (≠ A184), E194 (= E188), S330 (= S324), F448 (≠ L443), H450 (= H445), E455 (= E450)
binding flavin-adenine dinucleotide: I9 (≠ V11), G10 (= G12), A11 (≠ G13), G12 (= G14), P13 (= P15), G14 (= G16), I32 (≠ V34), E33 (= E35), K34 (= K36), Y35 (vs. gap), G46 (= G41), T47 (≠ I42), C48 (= C43), V51 (≠ W46), G52 (= G47), C53 (= C48), S56 (≠ T51), K57 (= K52), G119 (= G113), H120 (= H114), G121 (= G115), A149 (= A143), S150 (≠ T144), G151 (= G145), S170 (≠ Y164), I191 (= I185), R278 (≠ I273), G317 (= G311), D318 (= D312), M324 (≠ W318), L325 (= L319), A326 (= A320), H327 (= H321), A329 (= A323), Y357 (= Y352), H450 (= H445), P451 (= P446)
binding 1,4-dihydronicotinamide adenine dinucleotide: I186 (≠ V180), G187 (= G181), G189 (= G183), V190 (≠ A184), I191 (= I185), E194 (= E188), L209 (≠ V203), E210 (= E204), A211 (≠ M205), L212 (≠ A206), A275 (= A270), V276 (≠ A271), G277 (= G272), R278 (≠ I273), M324 (≠ W318), L325 (= L319), V355 (≠ C350), Y357 (= Y352)

Sites not aligning to the query:

active site: 473

5u8vA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to NAD+ (see paper)
41% identity, 99% coverage: 4:466/466 of query aligns to 1:470/472 of 5u8vA

query
sites
5u8vA

S

Q

S

K

F

F

D

D

L

V

I

V

V

V

V
x
I

G
|
G

G
x
A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

I

I

R

R

A

A

T

A

Q

Q

L

L

G

G

M

L

K

K

T

T

A

A

L

C

V
x
I

E
|
E

K
|
K

-
x
Y

-

I

-

G

-

K

-

E

-

G

-

K

A

V

H

A

L
|
L

G

G

G
|
G

I
x
T

C
|
C

L

L

N

N

W
x
V

G
|
G

C
|
C

I

I

P

P

T
x
S

K
|
K

A

A

L

L

R

D

S

S

A

S

D

Y

V

K

L

Y

R

H

L

E

V

A

K

K

D

E

A

A

-

F

A

K

A

V

Y

H

G

G

V

I

H

E

A

A

A

K

P
x
G

P
x
V

T

T

A

I

D

D

L

V

P

P

A

A

M

M

V

V

A

A

R

R

S

K

R

A

A

N

V

I

A

V

A

K

Q

N

L

L

N

T

K

G

G

G

V

I

A

A

H

T

L

L

M

F

K

K

K

A

N

N

G

G

V

V

T

T

V

S

L

F

N

E

G
|
G

H
|
H

G
|
G

R

K

L

L

A

L

G

A

K

N

G

K

K

Q

L

V

E

E

V

V

T

T

A

G

T

L

D

D

G

G

K

K

T

T

R

Q

I

V

V

L

S

E

S

A

A

E

H

N

I

V

L

I

A
|
A

T
x
S

G
|
G

A
x
S

R

R

A

P

R

V

Q

E

L

I

P

P

A

P

L

A

P

P

A

L

D

T

G

D

K

D

T

I

V

I

W

V

T

D

Y
x
S

R

T

D

G

A

A

L

L

A

E

P

F

P

Q

V

A

V

V

P

P

K

K

R

K

M

L

L

G

I

V

V
x
I

G
|
G

A

A

G
|
G

A
x
V

I
|
I

G

G

I

L

E
|
E

F

L

A

G

S

S

F

V

Y

W

R

A

A

R

V

L

G

G

A

A

E

E

V

V

T

T

V

V

V
x
L

E
|
E

M
x
A

A
x
L

Q

D

R

K

I

F

L

L

P

P

V

A

E

A

D

D

A

E

D

Q

I

I

C

A

A

K

Q

E

V

A

A

L

S

K

S

V

L

L

T

T

R

K

E

Q

G

G

I

L

V

N

L

I

H

R

T

L

G

G

T
x
A

G
x
R

I
x
V

Q

T

S

A

A

S

E

E

R

V

K

K

G

K

Q

K

G

Q

W

V

S

T

I

V

E

T

L

F

Q

T

G

D

G

A

K

-

S

N

G

G

G

E

A

Q

R

K

V

E

D

T

V

F

D

D

V

K

V

L

L

I

V

V

A
|
A

A
x
V

G
|
G

I
x
R

V

R

G

P

N

V

V

T

E

T

D

D

L

L

G

L

L

A

E

A

R

D

T

S

A

G

V

V

K

T

V

L

-

D

E

E

K

R

T

G

H

F

I

I

V

Y

T

V

D

D

G

D

F

H

G

C

R

K

T

T

D

S

E

V

P

P

G

G

V

V

Y

F

A

A

I

I

G
|
G

D
|
D

V

V

A

V

G

R

P

G

P

A

W
x
M

L
|
L

A
|
A

H
|
H

K

K

A
|
A

S
|
S

H

E

E

E

G

G

V

V

I

M

C

V

V

A

E

E

R

R

I

I

A

A

G

G

L

-

H

H

P

K

H

A

A

Q

L

M

D

N

A

Y

T

D

R

L

I

I

P

P

A

S

C

V

T

I

Y
|
Y

S

T

H

H

P

P

Q

E

V

I

A

A

S

W

V

V

G

G

L

K

T

T

E

E

A

Q

Q

T

A

L

R

K

E

A

R

E

G

G

H

V

P

E

V

V

K

N

V

V

G

G

K

T

F

F

P

P

F

F

A

A

G

A

N

S

G

G

K

R

A

A

I

M

A

A

M

A

G

N

S

D

T

T

A

T

G

G

L

L

V

V

K

K

V

V

V

I

F

A

D

D

A

A

G

K

S

T

G

D

E

R

L

V

L

L

G

G

A

V

H

H

M

V

V

I

G

G

D

P

E

S

V

A

T

A

E

E

M

L

I

V

Q

Q

G

Q

Y

G

A

A

I

I

A

G

Q

M

T

E

L

F

E

G

T

T

T

S

E

A

A

E

D

D

L

L

M

G

A

M

V

V

L
x
F

P

S

H
|
H

P
|
P

T

T

M

L

S

S

E
|
E

A

A

M

L

H

H

E

E

A

A

V

A

L

L

A

A

A

V

Y

N

G

G

R

H

A

A

L

I

H

H

I

I

active site: P12 (= P15), L43 (= L39), C47 (= C43), C52 (= C48), S55 (≠ T51), G81 (≠ P76), V82 (≠ P77), V189 (≠ A184), E193 (= E188), S329 (= S324), F447 (≠ L443), H449 (= H445), E454 (= E450)
binding flavin-adenine dinucleotide: I8 (≠ V11), G9 (= G12), A10 (≠ G13), G11 (= G14), P12 (= P15), G13 (= G16), I31 (≠ V34), E32 (= E35), K33 (= K36), Y34 (vs. gap), G45 (= G41), T46 (≠ I42), C47 (= C43), V50 (≠ W46), G51 (= G47), C52 (= C48), S55 (≠ T51), K56 (= K52), G118 (= G113), H119 (= H114), G120 (= G115), A148 (= A143), S149 (≠ T144), G150 (= G145), S151 (≠ A146), S169 (≠ Y164), I190 (= I185), R277 (≠ I273), G316 (= G311), D317 (= D312), M323 (≠ W318), L324 (= L319), A325 (= A320), H326 (= H321), A328 (= A323), Y356 (= Y352), H449 (= H445), P450 (= P446)
binding nicotinamide-adenine-dinucleotide: I185 (≠ V180), G186 (= G181), G188 (= G183), V189 (≠ A184), I190 (= I185), L208 (≠ V203), E209 (= E204), A210 (≠ M205), L211 (≠ A206), A241 (≠ T236), R242 (≠ G237), V243 (≠ I238), A274 (= A270), V275 (≠ A271), G276 (= G272)

Sites not aligning to the query:

active site: 472

P18925 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Azotobacter vinelandii (see 2 papers)
40% identity, 100% coverage: 3:466/466 of query aligns to 2:472/477 of P18925

query
sites
P18925

N

S

S

Q

S

K

F

F

D

D

L

V

I

I

V

V

V

I

G

G

G

A

G

G

P

P

G

G

Y

Y

V

V

A

A

I

I

R

K

A

S

T

A

Q

Q

L

L

G

G

M

L

K

K

T

T

A

A

L

L

V

I

E
|
E

K
|
K

A
x
Y

H
x
K

-
x
G

-
x
K

-
x
E

-
x
G

-
x
K

-
x
T

-
x
A

L
|
L

G
|
G

I
x
T

C
|
C

L

L

N

N

W

V

G

G

C
|
C

I

I

P

P

T

S

K
|
K

A

A

L

L

R

D

S

S

A

S

D

Y

V

K

L

F

R

H

L

E

V

A

K

H

D

E

A

S

-

F

A

K

A

L

Y

H

G

G

V

I

H

S

A

T

A

G

P

E

P

V

T

A

A

I

D

D

L

V

P

P

A

T

M

M

V

I

A

A

R

R

S

K

R

D

A

Q

V

I

A

V

A

R

Q

N

L

L

N

T

K

G

G

G

V

V

A

A

H

S

L

L

M

I

K

K

K

A

N

N

G

G

V

V

T

T

V

L

L

F

N

E

G

G

H

H

G

G

R

K

L

L

A

L

G

A

K

G

G

K

K

K

L

V

E

E

V

V

T

T

A

A

T

A

D

D

G

G

K

S

T

S

R

Q

I

V

V

L

S

D

S

T

A

E

H

N

I

V

L

I

L

L

A

A

T

S

G

G

A

S

R

K

A

P

R

V

Q

E

L

I

P

P

A

P

L

A

P

P

A

V

D

D

G

Q

K

D

T

V

V

I

W

V

T

D

Y

S

R

T

D

G

A

A

L

L

A

D

P

F

P

Q

V

N

V

V

P

P

K

G

R

K

M

L

L

G

I

V

V

I

G

G

A

A

G

G

A

V

I

I

G

G

I

L

E

E

F

L

A

G

S

S

F

V

Y

W

R

A

A

R

V

L

G

G

A

A

E

E

V

V

T

T

V

V

V

L

E

E

M

A

A

M

Q

D

R

K

I

F

L

L

P

P

V

A

E

V

D

D

A

E

D

Q

I

V

C

A

A

K

Q

E

V

A

A

Q

S

K

S

I

L

L

T

T

R

K

E

Q

G

G

I

L

V

K

L

I

H

L

T

L

G

G

T

A

G

R

I

V

Q

T

S

G

A

T

E

E

R

V

K

K

G

N

Q

K

G

Q

W

V

S

T

I

V

E

K

L

F

-

V

-

D

-

A

Q

E

G

G

G

E

K

K

S

S

G

Q

G

A

A

-

R

-

V

-

D

-

V

F

D

D

V

K

V

L

L

I

V

V

A

A

A

V

G

G

I

R

V

R

G

P

N

V

V

T

E

T

D

D

L

L

G

L

L

A

E

A

R

D

T

S

A

G

V

V

K

T

V

L

-

D

E

E

K

R

T

G

H

F

I

I

V

Y

T

V

D

D

G

D

F

Y

G

C

R

A

T

T

D

S

E

V

P

P

G

G

V

V

Y

Y

A

A

I

I

G

G

D
|
D

V

V

A

V

G

R

P

G

P

A

W

M

L

L

A
|
A

H

H

K

K

A

A

S

S

H

E

E

E

G

G

V

V

I

V

C

V

V

A

E

E

R

R

I

I

A

A

G

G

L

-

H

H

P

K

H

A

A

Q

L

M

D

N

A

Y

T

D

R

L

I

I

P

P

A

A

C

V

T

I

Y

Y

S

T

H

H

P

P

Q

E

V

I

A

A

S

G

V

V

G

G

L

K

T

T

E

E

A

Q

Q

A

A

L

R

K

E

A

R

E

G

G

H

V

P

A

V

I

K

N

V

V

G

G

K

V

F

F

P

P

F

F

A

A

G

A

N

S

G

G

K

R

A

A

I

M

A

A

M

A

G

N

S

D

T

T

A

A

G

G

L

F

V

V

K

K

V

V

V

I

F

A

D

D

A

A

G

K

S

T

G

D

E

R

L

V

L

L

G

G

A

V

H

H

M

V

V

I

G

G

D

P

E

S

V

A

T

A

E

E

M

L

I

V

Q

Q

G

Q

Y

G

A

A

I

I

A

A

Q

M

T

E

L

F

E

G

T

T

T

S

E

A

A

E

D

D

L

L

M

G

A

M

V

V

L

F

P

A

H

H

P

P

T

A

M

L

S

S

E

E

A

A

M

L

H

H

E

E

A

A

V

A

L

L

A

A

A

V

Y

S

G

G

R

H

A

A

L

I

H

H

I

V

34:49 (vs. 35:43, 38% identical) binding
C49 (= C43) modified: Disulfide link with 54, Redox-active
C54 (= C48) modified: Disulfide link with 49, Redox-active
K58 (= K52) binding
D319 (= D312) binding
A327 (= A320) binding

3ladA Refined crystal structure of lipoamide dehydrogenase from azotobacter vinelandii at 2.2 angstroms resolution. A comparison with the structure of glutathione reductase (see paper)
40% identity, 100% coverage: 3:466/466 of query aligns to 1:471/472 of 3ladA

query
sites
3ladA

N

S

S

Q

S

K

F

F

D

D

L

V

I

I

V

V

V
x
I

G
|
G

G
x
A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

I

I

R

K

A

S

T

A

Q

Q

L

L

G

G

M

L

K

K

T

T

A

A

L

L

V
x
I

E
|
E

K
|
K

A
x
Y

H

K

-

G

-

K

-

E

-

G

-

K

-

T

-

A

L
|
L

G

G

G
|
G

I
x
T

C
|
C

L

L

N

N

W
x
V

G
|
G

C
|
C

I

I

P

P

T
x
S

K
|
K

A

A

L

L

R

D

S

S

A

S

D

Y

V

K

L

F

R

H

L

E

V

A

K

H

D

E

A

S

-

F

A

K

A

L

Y

H

G

G

V

I

H

S

A

T

A

G

P

E

P

V

T

A

A

I

D

D

L

V

P

P

A

T

M

M

V

I

A

A

R

R

S

K

R

D

A

Q

V

I

A

V

A

R

Q

N

L

L

N

T

K

G

G

G

V

V

A

A

H

S

L

L

M

I

K

K

K

A

N

N

G

G

V

V

T

T

V

L

L

F

N

E

G
|
G

H
|
H

G
|
G

R

K

L

L

A

L

G

A

K

G

G

K

K

K

L

V

E

E

V

V

T

T

A

A

T

A

D

D

G

G

K

S

T

S

R

Q

I

V

V

L

S

D

S

T

A

E

H

N

I

V

L

I

L

L

A
|
A

T
x
S

G
|
G

A
x
S

R

K

A

P

R

V

Q

E

L

I

P

P

A

P

L

A

P

P

A

V

D

D

G

Q

K

D

T

V

V

I

W

V

T

D

Y
x
S

R

T

D

G

A

A

L

L

A

D

P

F

P

Q

V

N

V

V

P

P

K

G

R

K

M

L

L

G

I

V

V

I

G

G

A

A

G

G

A
x
V

I
|
I

G

G

I

L

E
|
E

F

L

A

G

S

S

F

V

Y

W

R

A

A

R

V

L

G

G

A

A

E

E

V

V

T

T

V

V

V

L

E

E

M

A

A

M

Q

D

R

K

I

F

L

L

P

P

V

A

E

V

D

D

A

E

D

Q

I

V

C

A

A

K

Q

E

V

A

A

Q

S

K

S

I

L

L

T

T

R

K

E

Q

G

G

I

L

V

K

L

I

H

L

T

L

G

G

T

A

G

R

I

V

Q

T

S

G

A

T

E

E

R

V

K

K

G

N

Q

K

G

Q

W

V

S

T

I

V

E

K

L

F

-

V

-

D

-

A

Q

E

G

G

G

E

K

K

S

S

G

Q

G

A

A

-

R

-

V

-

D

-

V

F

D

D

V

K

V

L

L

I

V

V

A

A

A

V

G

G

I
x
R

V

R

G

P

N

V

V

T

E

T

D

D

L

L

G

L

L

A

E

A

R

D

T

S

A

G

V

V

K

T

V

L

-

D

E

E

K

R

T

G

H

F

I

I

V

Y

T

V

D

D

G

D

F

Y

G

C

R

A

T

T

D

S

E

V

P

P

G

G

V

V

Y

Y

A

A

I

I

G
|
G

D
|
D

V

V

A

V

G

R

P

G

P

A

W
x
M

L
|
L

A
|
A

H
|
H

K

K

A
|
A

S

S

H

E

E

E

G

G

V

V

I

V

C

V

V

A

E

E

R

R

I

I

A

A

G

G

L

-

H

H

P

K

H

A

A

Q

L

M

D

N

A

Y

T

D

R

L

I

I

P

P

A

A

C

V

T

I

Y
|
Y

S

T

H

H

P

P

Q

E

V

I

A

A

S

G

V

V

G

G

L

K

T

T

E

E

A

Q

Q

A

A

L

R

K

E

A

R

E

G

G

H

V

P

A

V

I

K

N

V

V

G

G

K

V

F

F

P

P

F

F

A

A

G

A

N

S

G

G

K

R

A

A

I

M

A

A

M

A

G

N

S

D

T

T

A

A

G

G

L

F

V

V

K

K

V

V

V

I

F

A

D

D

A

A

G

K

S

T

G

D

E

R

L

V

L

L

G

G

A

V

H

H

M

V

V

I

G

G

D

P

E

S

V

A

T

A

E

E

M

L

I

V

Q

Q

G

Q

Y

G

A

A

I

I

A

A

Q

M

T

E

L

F

E

G

T

T

T

S

E

A

A

E

D

D

L

L

M

G

A

M

V

V

L
x
F

P

A

H
|
H

P
|
P

T

A

M

L

S

S

E
|
E

A

A

M

L

H

H

E

E

A

A

V

A

L

L

A

A

A

V

Y

S

G

G

R

H

A

A

L

I

H

H

I

V

active site: L44 (= L39), C48 (= C43), C53 (= C48), S56 (≠ T51), V190 (≠ A184), E194 (= E188), F448 (≠ L443), H450 (= H445), E455 (= E450)
binding flavin-adenine dinucleotide: I9 (≠ V11), G10 (= G12), A11 (≠ G13), G12 (= G14), P13 (= P15), G14 (= G16), I32 (≠ V34), E33 (= E35), K34 (= K36), Y35 (≠ A37), G46 (= G41), T47 (≠ I42), C48 (= C43), V51 (≠ W46), G52 (= G47), C53 (= C48), S56 (≠ T51), K57 (= K52), G119 (= G113), H120 (= H114), G121 (= G115), A149 (= A143), S150 (≠ T144), G151 (= G145), S152 (≠ A146), S170 (≠ Y164), I191 (= I185), R278 (≠ I273), G317 (= G311), D318 (= D312), M324 (≠ W318), L325 (= L319), A326 (= A320), H327 (= H321), A329 (= A323), Y357 (= Y352), H450 (= H445), P451 (= P446)

6awaA 1.83 angstrom resolution crystal structure of dihydrolipoyl dehydrogenase from pseudomonas putida in complex with fad and adenosine-5'-monophosphate.
40% identity, 99% coverage: 6:466/466 of query aligns to 5:472/475 of 6awaA

query
sites
6awaA

F

F

D

D

L

V

I

V

V

V

V
x
I

G
|
G

G
x
A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

I

I

R

K

A

A

T

A

Q

Q

L

L

G

G

M

L

K

K

T

T

A

A

L

C

V
x
I

E
|
E

K
|
K

A

Y

H

T

-

D

-

A

-

E

-

G

-

K

-

L

-

A

L
|
L

G

G

G
|
G

I
x
T

C
|
C

L

L

N

N

W
x
V

G
|
G

C
|
C

I

I

P

P

T
x
S

K
|
K

A

A

L

L

R

D

S

S

A

S

D

W

V

K

L

Y

R

K

L

E

V

A

K

K

D

E

A

S

-

F

A

N

A

V

Y

H

G

G

V

I

H

S

A

T

A

G

P

E

P

V

T

K

A

M

D

D

L

V

P

A

A

A

M

M

V

V

A

G

R

R

S

K

R

A

A

G

V

I

A

V

A

K

Q

N

L

L

N

T

K

G

G

G

V

V

A

A

H

T

L

L

M

F

K

K

K

A

N

N

G

G

V

V

T

T

V

S

L

I

N

Q

G
|
G

H
|
H

G
|
G

R

K

L

L

A

L

G

A

K

G

G

K

K

K

L

V

E

E

V

V

T

T

A

K

T

A

D

D

G

G

K

T

T

T

R

E

I

V

V

I

S

E

S

A

A

E

H

N

I

V

L

I

L

L

A
|
A

T
x
S

G
|
G

A
x
S

R

R

A

P

R

I

Q

D

L

I

P

P

A

P

L

A

P

P

A

V

D

D

G

Q

K

N

T

V

V

I

W

V

T

D

Y
x
S

R

T

D

G

A

A

L

L

A

E

P

F

P

Q

V

A

V

V

P

P

K

K

R

R

M

L

L

G

I

V

V
x
I

G
|
G

A

A

G
|
G

A
x
V

I
|
I

G

G

I

L

E
|
E

F

L

A

G

S

S

F

V

Y

W

R

A

A

R

V

L

G

G

A

A

E

E

V

V

T

T

V

V

V
x
L

E
|
E

M
x
A

A
x
L

Q

D

R

T

I

F

L

L

P

M

V

A

E

A

D

D

A

T

D

A

I

V

C

S

A

K

Q

E

V

A

A

Q

S

K

S

T

L

L

T

T

R

K

E

Q

G

G

I

L

V

D

L

I

H

K

T

L

G

G

T
x
A

G
x
R

I
x
V

Q

T

S

G

A

S

E

K

R

V

K

N

G

G

Q

N

G

E

W

V

S

E

I

V

E

T

L

Y

Q

T

G

N

G

A

K

E

S

-

G

G

G

E

A

Q

R

K

V

I

D

T

V

F

D

D

V

K

V

L

L

I

V

V

A
|
A

A
x
V

G
|
G

I
x
R

V

R

G

P

N

V

V

T

E

T

D

D

L

L

G

L

L

A

E

S

R

D

T

S

A

G

V

V

K

T

V

I

-

D

E

E

K

R

T

G

H

Y

I

I

V

F

T

V

D

D

G

D

F

Y

G

C

R

A

T

T

D

S

E

V

P

P

G

G

V

V

Y

Y

A

A

I

I

G
|
G

D
|
D

V

V

A

V

G

R

P

G

P

M

W
x
M

L
|
L

A
|
A

H
|
H

K

K

A
|
A

S

S

H

E

E

E

G

G

V

I

I

M

C

V

V

V

E

E

R

R

I

I

A

K

G

G

L

-

H

H

P

K

H

A

A

Q

L

M

D

N

A

Y

T

D

R

L

I

I

P

P

A

S

C

V

T

I

Y
|
Y

S

T

H

H

P

P

Q

E

V

I

A

A

S

W

V

V

G

G

L

K

T

T

E

E

A

Q

Q

A

A

L

R

K

E

A

R

E

G

G

H

V

P

E

V

V

K

N

V

V

G

G

K

T

F

F

P

P

F

F

A

A

G

A

N

S

G

G

K

R

A

A

I

M

A

A

M

A

G

N

S

D

T

T

A

G

G

G

L

F

V

V

K

K

V

V

V

I

F

A

D

D

A

A

G

K

S

T

G

D

E

R

L

V

L

L

G

G

A

V

H

H

M

V

V

I

G

G

D

P

E

S

V

A

T

A

E

E

M

L

I

V

Q

Q

G

Q

Y

G

A

A

I

I

A

A

Q

M

T

E

L

F

E

G

T

T

T

S

E

A

A

E

D

D

L

L

M

G

A

M

V

V

L
x
F

P

S

H
|
H

P
|
P

T

T

M

L

S

S

E
|
E

A

A

M

L

H

H

E

E

A

A

V

A

L

L

A

A

A

V

Y

N

G

G

R

G

A

A

L

I

H

H

I

V

active site: L45 (= L39), C49 (= C43), C54 (= C48), S57 (≠ T51), V191 (≠ A184), E195 (= E188), F449 (≠ L443), H451 (= H445), E456 (= E450)
binding adenosine monophosphate: I187 (≠ V180), G188 (= G181), G190 (= G183), L210 (≠ V203), E211 (= E204), A212 (≠ M205), L213 (≠ A206), A243 (≠ T236), R244 (≠ G237), V245 (≠ I238), A276 (= A270), V277 (≠ A271), G278 (= G272)
binding flavin-adenine dinucleotide: I10 (≠ V11), G11 (= G12), A12 (≠ G13), G13 (= G14), P14 (= P15), G15 (= G16), I33 (≠ V34), E34 (= E35), K35 (= K36), G47 (= G41), T48 (≠ I42), C49 (= C43), V52 (≠ W46), G53 (= G47), C54 (= C48), S57 (≠ T51), K58 (= K52), G120 (= G113), H121 (= H114), G122 (= G115), A150 (= A143), S151 (≠ T144), G152 (= G145), S153 (≠ A146), S171 (≠ Y164), I192 (= I185), R279 (≠ I273), G318 (= G311), D319 (= D312), M325 (≠ W318), L326 (= L319), A327 (= A320), H328 (= H321), A330 (= A323), Y358 (= Y352), H451 (= H445), P452 (= P446)

Sites not aligning to the query:

active site: 474, 475

P31023 Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; Pyruvate dehydrogenase complex E3 subunit; E3; PDC-E3; EC 1.8.1.4 from Pisum sativum (Garden pea) (see 2 papers)
39% identity, 99% coverage: 7:466/466 of query aligns to 39:501/501 of P31023

query
sites
P31023

D

D

L

V

I

V

V

I

G

G

P

P

G

G

Y

Y

V

V

A

A

I

I

R

K

A

A

T

A

Q

Q

L

L

G

G

M

F

K

K

T

T

A

T

L

C

V

I

E
|
E

K
|
K

-
x
R

A
x
G

H
x
A

L
|
L

G
|
G

I
x
T

C
|
C

L

L

N

N

W

V

G

G

C
|
C

I

I

P

P

T

S

K

K

A

A

L

L

R

H

S

S

A

S

D

H

V

M

L

Y

R

H

L

E

V

A

K

K

D

H

A

S

-

F

A

A

A

N

Y

H

G

G

V

V

H

K

A

V

A

S

P

N

P

V

T

E

A

I

D

D

L

L

P

A

A

A

M

M

V

M

A

G

R

Q

S

K

R

D

A

K

V

A

A

V

A

S

Q

N

L

L

N

T

K

R

G

G

V

I

A

E

H

G

L

L

M

F

K

K

N

N

G

K

V

V

T

T

V

Y

L

V

N

K

G

G

H

Y

G
|
G

R

K

L

F

A

V

G

S

K

P

G

S

K

E

L

I

E

S

V

V

T

D

A

T

T

I

D

E

G

G

K

E

T

N

R

T

I

V

V

V

S

K

S

G

A

K

H

H

I

I

L

I

A

A

T

T

G

G

A

S

R

D

A

V

R

K

Q

S

L

L

P

P

A

G

L

V

P

T

A

I

D

D

G

E

K

K

T

K

V

I

W

V

T

S

Y

S

R

T

D

G

A

A

L

L

A

A

P

L

P

S

V

E

V

I

P

P

K

K

R

K

M

L

L

V

I

V

V

I

G

G

A

A

G

G

A

Y

I

I

G

G

I

L

E

E

F

M

A

G

S

S

F

V

Y

W

R

G

A

R

V

I

G

G

A

S

E

E

V

V

T

T

V

V

E

E

M

F

A

A

Q

S

R

E

I

I

L

V

P

P

V

T

E

M

D

D

A

A

D

E

I

I

C

R

A

K

Q

Q

V

F

A

Q

S

R

S

S

L

L

T

E

R

K

E

Q

G

G

I

M

V

K

L

F

H

K

T

L

G

K

T

T

G

K

I

V

Q

V

S

G

A

V

E

D

R

T

K

S

G

G

Q

D

G

G

W

V

S

K

I

L

E

T

L

V

Q

E

G

P

G

S

K

-

S

A

G

G

A

E

R

Q

-

T

-

I

V

I

D

E

V

A

D

D

V

V

L

L

V

V

A

S

A

A

G

G

I

R

V

T

G

P

N

F

V

T

E

S

D

G

L

L

G

N

L

L

E

D

R

K

T

I

A

G

V

V

K

E

V

T

E

D

K

K

T

L

-

G

H

R

I

I

V

L

T

V

D

N

G

E

F

R

G

F

R

S

T

T

D

N

E

V

P

S

G

G

V

V

Y

Y

A

A

I

I

G

G

D
|
D

V

V

A

I

G

P

P

G

P

P

W
x
M

L
|
L

A
|
A

H
|
H

K

K

A

A

S

E

H

E

E

D

G

G

V

V

I

A

C

C

V

V

E

E

R

Y

I

L

A

A

G

G

L

K

H

V

P

G

H

H

A

-

L

V

D

D

A

Y

T

D

R

K

I

V

P

P

A

G

C

V

T

V

Y

Y

S

T

H

N

P

P

Q

E

V

V

A

A

S

S

V

V

G

G

L

K

T

T

E

E

A

E

Q

Q

A

V

R

K

E

E

R

T

G

G

H

V

P

E

V

Y

K

R

V

V

G

G

K

K

F

F

P

P

F

F

A

M

G

A

N

N

G

S

K

R

A

A

I

K

A

A

M

I

G

D

S

N

T

A

A

E

G

G

L

L

V

V

K

K

V

I

F

A

D

E

A

K

G

E

S

T

G

D

E

K

L

I

L

L

G

G

A

V

H

H

M

I

V

M

G

A

D

P

E

N

V

A

T

G

E

E

M

L

I

I

Q

H

G

E

Y

A

A

A

I

I

A

A

Q

L

T

Q

L

Y

E

D

T

A

T

S

E

S

A

E

D

D

L

I

M

A

A

R

A

V

V

C

L

H

P

A

H

H

P

P

T

T

M

M

S

S

E

E

A

A

M

I

H

K

E

E

A

A

V

A

L

M

A

A

A

T

Y

Y

G

D

R

K

A

P

L

I

H

H

I

I

67:76 (vs. 35:43, 60% identical) binding
C76 (= C43) modified: Disulfide link with 81, Redox-active
C81 (= C48) modified: Disulfide link with 76, Redox-active
G149 (= G115) binding
D348 (= D312) binding
MLAH 354:357 (≠ WLAH 318:321) binding

Sites not aligning to the query:

1:31 modified: transit peptide, Mitochondrion

1dxlA Dihydrolipoamide dehydrogenase of glycine decarboxylase from pisum sativum (see paper)
39% identity, 99% coverage: 7:466/466 of query aligns to 5:467/467 of 1dxlA

query
sites
1dxlA

D

D

L

V

I

V

V

I

V
x
I

G

G

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

I

I

R

K

A

A

T

A

Q

Q

L

L

G

G

M

F

K

K

T

T

A

T

L

C

V
x
I

E
|
E

K
|
K

-
x
R

A

G

H

A

L
|
L

G

G

G
|
G

I
x
T

C
|
C

L

L

N

N

W
x
V

G
|
G

C
|
C

I

I

P

P

T
x
S

K
|
K

A

A

L

L

R

H

S

S

A

S

D

H

V

M

L

Y

R

H

L

E

V

A

K

K

D

H

A

S

-

F

A

A

A

N

Y

H

G

G

V

V

H

K

A

V

A

S

P

N

P

V

T

E

A

I

D

D

L

L

P

A

A

A

M

M

V

M

A

G

R

Q

S

K

R

D

A

K

V

A

A

V

A

S

Q

N

L

L

N

T

K

R

G

G

V

I

A

E

H

G

L

L

M

F

K

K

N

N

G

K

V

V

T

T

V

Y

L

V

N

K

G
|
G

H
x
Y

G
|
G

R

K

L

F

A

V

G

S

K

P

G

S

K

E

L

I

E

S

V

V

T

D

A

T

T

I

D

E

G

G

K

E

T

N

R

T

I

V

V

V

S

K

S

G

A

K

H

H

I

I

L

I

A
|
A

T
|
T

G
|
G

A
x
S

R

D

A

V

R

K

Q

S

L

L

P

P

A

G

L

V

P

T

A

I

D

D

G

E

K

K

T

K

V

I

W

V

T

S

Y
x
S

R

T

D

G

A

A

L

L

A

A

P

L

P

S

V

E

V

I

P

P

K

K

R

K

M

L

L

V

I

V

V

I

G

G

A

A

G

G

A
x
Y

I
|
I

G

G

I

L

E
|
E

F

M

A

G

S

S

F

V

Y

W

R

G

A

R

V

I

G

G

A

S

E

E

V

V

T

T

V

V

E

E

M

F

A

A

Q

S

R

E

I

I

L

V

P

P

V

T

E

M

D

D

A

A

D

E

I

I

C

R

A

K

Q

Q

V

F

A

Q

S

R

S

S

L

L

T

E

R

K

E

Q

G

G

I

M

V

K

L

F

H

K

T

L

G

K

T

T

G

K

I

V

Q

V

S

G

A

V

E

D

R

T

K

S

G

G

Q

D

G

G

W

V

S

K

I

L

E

T

L

V

Q

E

G

P

G

S

K

-

S

A

G

G

A

E

R

Q

-

T

-

I

V

I

D

E