SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS21960 FitnessBrowser__Cup4G11:RR42_RS21960 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
53% identity, 98% coverage: 3:302/305 of query aligns to 4:305/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K42), S266 (= S264), P293 (≠ Y290)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K42), T71 (= T69), S72 (= S70), G73 (= G71), N74 (= N72), T75 (= T73), Q144 (= Q142), G176 (= G174), V177 (= V175), G178 (= G176), T179 (= T177), G180 (= G178), G181 (= G179), T182 (≠ H180), Q221 (= Q219), G222 (= G220), I223 (= I221), S266 (= S264), P293 (≠ Y290), D294 (= D291)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
53% identity, 98% coverage: 3:302/305 of query aligns to 4:305/310 of P9WP55

query
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P9WP55

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K44 (= K42) modified: N6-(pyridoxal phosphate)lysine
N74 (= N72) binding
GTGGT 178:182 (≠ GTGGH 176:180) binding
S266 (= S264) binding

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
54% identity, 97% coverage: 3:297/305 of query aligns to 4:300/300 of 3zeiA

query
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3zeiA

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active site: K44 (= K42), S266 (= S264), P293 (≠ Y290)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T69), S72 (= S70), G73 (= G71), T75 (= T73), M122 (= M120), I126 (= I124), Q144 (= Q142), F145 (= F143), K215 (≠ P213), G222 (= G220), G224 (= G222), A225 (= A223), F227 (= F225)
binding pyridoxal-5'-phosphate: V43 (≠ I41), K44 (= K42), N74 (= N72), G176 (= G174), V177 (= V175), G178 (= G176), T179 (= T177), G180 (= G178), G181 (= G179), T182 (≠ H180), Q221 (= Q219), G222 (= G220), I223 (= I221), S266 (= S264), P293 (≠ Y290), D294 (= D291)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
54% identity, 97% coverage: 3:297/305 of query aligns to 4:300/300 of 2q3cA

query
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2q3cA

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G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K42), S266 (= S264), P293 (≠ Y290)
binding : K44 (= K42), T71 (= T69), S72 (= S70), G73 (= G71), N74 (= N72), T75 (= T73), G121 (= G119), M122 (= M120), Q144 (= Q142), F145 (= F143), G178 (= G176), K215 (≠ P213), Q221 (= Q219), G222 (= G220), G224 (= G222), A225 (= A223)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
51% identity, 98% coverage: 3:302/305 of query aligns to 4:308/318 of 4lmaA

query
sites
4lmaA

A

A

D

H

N

D

I

V

L

T

Q

E

T

L

I

V

G

G

N

R

T

T

P

P

H

L

I

V

R

Q

V

L

Q

N

R

R

L

I

-

P

-

Q

-

A

F

E

G

G

D

C

T

L

H

G

Q

R

V

I

W

V

I

M

K

K

S

L

E

E

R

G

A

M

N

N

P

P

G

A

S

S

I
x
V

K
|
K

D

D

R

R

I

I

A

G

L

T

S

H

M

M

V

I

E

N

A

S

A

A

E

E

Q

K

S

A

G

G

V

L

L

I

K

N

P

P

G

E

G

T

T

T

I

V

-

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

M

M

V

T

A

A

V

A

K

K

G

G

Y

Y

K

R

L

L

V

I

L

L

V

T

M

M

P

P

D

E

S

T

M

M

S

S

V

L

E

E

R

R

R

A

L

M

M

L

L

K

A

A

Y

Y

G

G

A

A

T

T

F

L

D

E

L

L

T

T

P

P

R

G

E

S

K

Q

G

G

M

M

K

K

G

G

A

A

I

I

A

L

R

R

A

A

E

Q

L

I

V

V

A

D

A

S

T

I

P

P

G

G

A

A

W

Y

M

M

P

L

Q

Q

F

F

E

R

N

N

P

P

A

S

N

N

I

P

E

E

A

I

H

H

V

R

N

L

T

T

A

A

L

E

E

E

I

I

L

W

A

Q

D

D

F

T

P

E

Q

G

G

Q

I

V

D

D

A

F

L

I

V

V

T

A

G
|
G

V
|
V

G
|
G

T
|
T

G
|
G

H
x
T

I

I

T

T

G

G

C

V

A

A

R

E

V

V

L

I

K

K

Q

S

K

R

W

K

P

P

A

S

L

F

K

Q

V

V

Y

V

A

A

V

V

E

E

P

P

V

F

A

N

S

S

P

P

V

V

I

I

S

S

G

G

A

N

P

P

A

G

P

P

H

H

P

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A

A

G

G

F

F

I

I

P

P

R

E

N

V

L

L

D

R

T

T

A

D

L

L

L

I

D

D

G

E

V

V

I

I

Q

T

V

V

D

S

A

D

E

E

P

E

A

A

R

F

E

Q

M

F

A

G

R

R

A

L

A

A

R

K

E

E

G

G

M

L

L

L

V

S

G

G

I

I

S
|
S

S

S

G

G

A

A

T

N

L

L

A

C

A

A

I

-

A

A

Q

I

K

Q

L

L

P

A

D

Q

L

R

P

P

-

E

-

N

A

E

G

G

A

K

T

L

V

I

L

V

G

V

F

I

N

Q

Y
x
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

T

S

V

T

E

L

G

M

F

F

active site: K46 (= K42), S269 (= S264)
binding pyridoxal-5'-phosphate: V45 (≠ I41), K46 (= K42), N77 (= N72), G179 (= G174), V180 (= V175), G181 (= G176), T182 (= T177), G183 (= G178), G184 (= G179), T185 (≠ H180), Q224 (= Q219), G225 (= G220), I226 (= I221), S269 (= S264), P296 (≠ Y290), S297 (≠ D291)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
52% identity, 98% coverage: 3:302/305 of query aligns to 4:308/310 of 4lmbA

query
sites
4lmbA

A

A

D

R

N

D

I

I

L

T

Q

Q

T

L

I

V

G

G

N

R

T

T

P

P

H

L

I

V

R

Q

V

L

Q

N

R

R

L

I

-

P

-

V

-

A

F

E

G

G

D

V

T

K

H

A

Q

R

V

I

W

V

I

V

K

K

S

L

E

E

R

S

A

M

N

N

P

P

G

A

S

S

I

V

K
|
K

D

D

R

R

I

I

A

G

L

V

S

S

M

M

V

V

E

E

A

D

A

A

E

E

Q

A

S

A

G

G

V

L

L

I

K

H

P

P

G

D

G

K

T

T

I

I

-

L

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

M

M

V

V

A

A

V

A

K

K

G

G

Y

Y

K

R

L

L

V

V

L

L

V

T

M

M

P

P

D

E

S

T

M
|
M

S

S

V

L

E

E

R

R

R

A

L

M

M

L

L

K

A

A

Y

Y

G

G

A

A

T

Q

F

L

D

E

L

L

T

T

P

P

R

G

E

S

K

Q

G

G

M
|
M

K

E

G

G

A

A

I

I

A

T

R

R

A

A

E

E

L

I

V

V

A

E

A

N

T

T

P

P

G

N

A

A

W

Y

M

S

P

L

Q

Q

Q
|
Q

F
|
F

E

R

N

N

P

P

A

A

N

N

I

P

E

K

A

I

H

H

V

R

N

E

T

T

A

A

L

E

E

E

I

I

L

W

A

A

D

D

F

T

P

D

Q

G

G

L

I

V

D

D

A

I

L

V

V

I

T

G

G
|
G

V
|
V

G
|
G

T
|
T

G
|
G

H
x
T

I

I

T

T

G

G

C

I

A

A

R

E

V

T

L

I

K

K

Q

P

K

R

W

R

P

P

A

Q

L

F

K

Q

V

A

Y

I

A

A

V

V

E

E

P

P

V

S

A

N

S

S

P

P

V

V

I

L

S

S

G

G

A

Q

P

P

A

G

P

P

H

H

P

K

I
|
I

Q
|
Q

G
|
G

I
|
I

G
|
G

A
|
A

G

G

F

F

I

I

P

P

R

A

N

I

L

F

D

R

T

P

A

E

L

L

L

I

D

D

G

E

V

V

I

I

Q

I

V

V

D

D

A

D

E

T

P

E

A

A

R

F

E

A

M

Y

A

A

R

R

A

L

A

A

R

R

E

Q

E

E

G

G

M

L

L

L

V

S

G

G

I

I

S
|
S

S

A

G

G

A

A

T

A

L

L

A

W

A

A

I

A

A

I

Q

Q

-

V

-

G

K

K

L

R

P

P

D

E

L

N

P

E

A

D

G

K

A

L

T

I

V

V

L

M

G

-

F

I

N

Q

Y
x
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

T

S

V

T

E

A

G

L

F

F

active site: K46 (= K42), S269 (= S264)
binding cysteine: K46 (= K42), T74 (= T69), S75 (= S70), S75 (= S70), G76 (= G71), N77 (= N72), T78 (= T73), M101 (= M96), M125 (= M120), M125 (= M120), Q147 (= Q142), F148 (= F143), F148 (= F143), I223 (= I218), Q224 (= Q219), G225 (= G220), G225 (= G220), I226 (= I221), G227 (= G222), A228 (= A223)
binding pyridoxal-5'-phosphate: K46 (= K42), N77 (= N72), G179 (= G174), V180 (= V175), G181 (= G176), T182 (= T177), G183 (= G178), G184 (= G179), T185 (≠ H180), Q224 (= Q219), G225 (= G220), I226 (= I221), S269 (= S264), P296 (≠ Y290), S297 (≠ D291)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
50% identity, 98% coverage: 3:302/305 of query aligns to 4:306/309 of 7n2tA

query
sites
7n2tA

A

A

D

K

N

D

I

V

L

T

Q

E

T

L

I

I

G

G

N

N

T

T

P

P

H

L

I

V

R

Y

V

L

Q

N

R

R

L

V

F

V

-

D

G

G

D

C

T

V

H

A

Q

R

V

V

W

A

I

A

K

K

S

L

E

E

R

M

A

M

N

E

P

P

G

C

G

S

S

S

I

V

K

K

D

D

R

R

I

I

A

G

L

Y

S

S

M

M

V

I

E

S

A

D

A

A

E

E

Q

N

S

K

G

G

V

L

L

I

K

T

P

P

G

G

G

E

T

S

I

V

-

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

M

F

V

I

A

A

V

A

K

K

G

G

Y

Y

K

R

L

L

V

I

L

I

V

C

M

M

P

P

D

A

S

S

M

M

S

S

V

L

E

E

R

R

R

T

L

I

M

L

L

R

A

A

Y

F

G

G

A

A

T

E

F

L

D

V

L

L

T

T

P

D

R

P

E

A

K

R

G

G

M

M

K

K

G

G

A

A

I

V

A

Q

R

K

A

A

E

E

L

I

V

K

A

A

A

K

T

T

P

P

G

N

A

S

W

Y

M

I

P

L

Q

Q

F

F

E

E

N

N

P

P

A

A

N

N

I

P

E

K

A

I

H

H

V

Y

N

E

T

T

A

G

L

P

E

E

I

I

L

W

A

R

D

G

F

S

P

G

Q

G

G

K

I

I

D

D

A

A

L

L

V

V

T

S

G

G

V

I

G

G

T

T

G

G

H

T

I

V

T

T

G

G

C

A

A

G

R

K

V

Y

L

L

K

K

Q

E

K

Q

W

N

P

P

A

N

L

I

K

K

V

L

Y

Y

A

G

V

V

E

E

P

P

V

V

A

E

S

S

P

A

V

I

I

L

S

S

G

G

A

K

P

P

A

G

P

P

H

H

P

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

R

G

N

V

L

L

D

D

T

V

A

N

L

L

D

D

G

E

V

V

I

I

Q

Q

V

V

D

S

A

S

E

E

P

E

A

S

R

I

E

E

M

T

A

A

R

K

R

L

A

L

A

A

R

L

E

K

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S

S

G

G

-

A

A

A

T

A

L

A

A

A

A

I

I
x
R

A

I

Q

A

K

K

L
x
R

P

P

D

E

L

-

P

N

A

A

G

G

A

K

T

L

V

I

L

V

G

A

F

V

N

F

Y

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

T

S

V

T

E

V

G

L

F

F

binding phosphate ion: R277 (≠ I272), R281 (≠ L276)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
50% identity, 98% coverage: 3:302/305 of query aligns to 76:378/392 of P47999

query
sites
P47999

A

A

D

D

N

N

I

A

L

A

Q

Q

T

L

I

I

G

G

N

K

T

T

P

P

H

M

I

V

R

Y

V

L

Q

N

R

N

L

V

F

V

-

K

G

G

D

C

T

V

H

A

Q

S

V

V

W

A

I

A

K

K

S

L

E

E

R

I

A

M

N

E

P

P

G

C

S

S

I

V

K

K

D

D

R

R

I

I

A

G

L

Y

S

S

M

M

V

I

E

T

A

D

A

A

E

E

Q

E

S

K

G

G

V

L

L

I

K

T

P

P

G

G

G

K

T

S

I

V

-

L

I

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

M

F

V

I

A

A

V

S

K

K

G

G

Y

Y

K

K

L

L

V

I

L

L

V

T

M

M

P

P

D

A

S

S

M

M

S

S

V

L

E

E

R

R

R

V

L

L

M

L

L

R

A

A

Y

F

G

G

A

A

T

E

F

L

D

V

L

L

T

T

P

E

R

P

E

A

K

K

G

G

M

M

K

T

G

G

A

A

I

I

A

Q

R

K

A

A

E

E

L

I

V

L

A

K

T

T

P

P

G

N

A

S

W

Y

M

M

P

L

Q

Q

F

F

E

D

N

N

P

P

A

A

N

N

I

P

E

K

A

I

H

H

V

Y

N

E

T

T

A

G

L

P

E

E

I

I

L

W

A

E

D

D

F

T

P

R

Q

G

G

K

I

I

D

D

A

I

L

L

V

V

T

A

G

G

V

I

G

G

T

T

G

G

H

T

I

I

T

T

G

G

C

V

A

G

R

R

V

F

L

I

K

K

Q

E

K

R

W

K

P

P

A

E

L

L

K

K

V

V

Y

I

A

G

V

V

E

E

P

P

V

T

A

E

S

S

P

A

V

I

I

L

S

S

G

G

A

K

P

P

A

G

P

P

H

H

P

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

V

P

P

R

K

N

N

L

L

D

D

T

L

A

A

L

I

L

V

D

D

G

E

V

Y

I

I

Q

A

V

I

D

S

A

S

E

E

P

E

A

A

R

I

E

E

M

T

A

S

R

K

R

Q

A

L

A

A

R

L

E

Q

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S

S

G

G

A

A

T

A

L

A

A

A

I

A

A

I

Q

Q

-

V

-

A

K

K

L

R

P

P

D

E

L

-

P

N

A

A

G

G

A

K

T

L

V

I

L

A

G

V

F

V

N

F

Y

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

T

S

V

T

E

Q

G

L

F

F

Sites not aligning to the query:

61 modified: N-acetylalanine

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
50% identity, 98% coverage: 3:302/305 of query aligns to 6:308/322 of P47998

query
sites
P47998

A

A

D

K

N

D

I

V

L

T

Q

E

T

L

I

I

G

G

N

N

T

T

P

P

H

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I

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R

Y

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L

Q

N

R

N

L

V

F

A

-

E

G

G

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H

G

Q

R

V

V

W

A

I

A

K

K

S

L

E

E

R

M

A

M

N

E

P

P

G

C

G

S

S

S

I

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K
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K

D

D

R

R

I

I

A

G

L

F

S

S

M

M

V

I

E

S

A

D

A

A

E

E

Q

K

S

K

G

G

V

L

L

I

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K

P

P

G

G

G

E

T

S

I

V

-

L

I

I

E

E

P

P

T
|
T

S
|
S

G

G

N
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N

T
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T

G

G

I

V

G

G

L

L

A

A

M

F

V

T

A

A

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A

K

K

G

G

Y

Y

K

K

L

L

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I

L

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T

M

M

P

P

D

A

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S

M

M

S

S

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E

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I

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L

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A

A

Y

F

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D

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P

E

A

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K

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M

M

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G

A

A

I

I

A

A

R

K

A

A

E

E

L

I

V

L

A

A

A

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T

T

P

P

G

N

A

G

W

Y

M

M

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L

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Q

Q
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Q

F

F

E

E

N

N

P

P

A

A

N

N

I

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A

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H
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H

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Y

N

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A
x
G

L

P

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S

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G

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T

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G

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x
T

I

I

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T

G

G

C

A

A

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L

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N

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A

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Y

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E

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A

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I

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S

S

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G

A
x
K

P

P

A

G

P

P

H
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H

P
x
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

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S

N

V

L

L

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N

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A

D

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L

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D

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Q

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E

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M

A

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A

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A

A

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K

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E

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M

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G

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S
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S

S

S

G

G

A

A

T

A

L

A

A

A

I

A

A

I

Q

-

K

K

L

L

P

A

D

Q

L

R

P

P

-

E

-

N

A

A

G

G

A

K

T

L

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F

L

V

G

A

F

I

N

F

Y

P

D

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E

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R

Y

Y

L

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T

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L

F

F

K46 (= K42) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T69) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S70) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N72) binding ; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T73) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q142) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H152) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (≠ A157) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTGGH 176:180) binding
T182 (= T177) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (≠ H180) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ A212) mutation to A: Impaired interaction with SAT1.
H221 (= H216) mutation to A: Impaired interaction with SAT1.
K222 (≠ P217) mutation to A: Impaired interaction with SAT1.
S269 (= S264) binding ; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
50% identity, 98% coverage: 3:302/305 of query aligns to 4:306/320 of 2isqA

query
sites
2isqA

A

A

D

K

N

D

I

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L

T

Q

E

T

L

I

I

G

G

N

N

T

T

P

P

H

L

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Y

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N

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N

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F

A

-

E

G

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A

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A

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A

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-

L

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T

S
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S

G
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G

N
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N

T
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T

G

G

I

V

G

G

L

L

A

A

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F

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T

A

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A

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K

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Y

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A

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A

A

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P

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N

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G

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E

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N

N

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A

A

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G
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x
I

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T

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T

G

G

C

A

A

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W

N

P

A

A

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Y

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S

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P

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A
x
G

P
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P

H
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H

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I

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Q

G
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G

I
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I

G
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G

A
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A

G

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F
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F

I

I

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S

N

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M

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A

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A

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K

E

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S

S

S

G

G

A

A

T

A

L

A

A

A

I

A

A

I

Q

-

K

K

L

L

P

A

D

Q

L

R

P

P

-

E

-

N

A

A

G

G

A

K

T

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L

V

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A

F

I

N

F

Y
x
P

D
x
S

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F

active site: K44 (= K42), S267 (= S264)
binding pyridoxal-5'-phosphate: K44 (= K42), N75 (= N72), G177 (= G174), I178 (≠ V175), G179 (= G176), T180 (= T177), G181 (= G178), G182 (= G179), T183 (≠ H180), Q222 (= Q219), G223 (= G220), I224 (= I221), S267 (= S264), P294 (≠ Y290), S295 (≠ D291)
binding : K44 (= K42), T72 (= T69), S73 (= S70), G74 (= G71), N75 (= N72), T76 (= T73), S98 (= S95), K121 (= K118), G122 (= G119), M123 (= M120), K124 (= K121), Q145 (= Q142), F146 (= F143), G179 (= G176), G217 (≠ A214), P218 (= P215), H219 (= H216), Q222 (= Q219), G223 (= G220), G225 (= G222), A226 (= A223), F228 (= F225)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
50% identity, 98% coverage: 3:302/305 of query aligns to 4:306/320 of 1z7yA

query
sites
1z7yA

A

A

D

K

N

D

I

V

L

T

Q

E

T

L

I

I

G

G

N

N

T

T

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P

H

L

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Y

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N

R

N

L

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F

A

-

E

G

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A

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A

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K

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M

A

M

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P

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C

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S

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x
A

D

D

R

R

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I

A

G

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S

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M

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S

A

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A

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S

K

G

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K

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P

G

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-

L

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P

T
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T

S

S

G
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G

N
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N

T
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T

G

G

I

V

G

G

L

L

A

A

M

F

V

T

A

A

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A

K

K

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G

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Y

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K

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L

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I

L

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M

M

P

P

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M

S

S

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L

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L

A

A

Y

F

G

G

A

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F

L

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V

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T

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D

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P

E

A

K

K

G

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M

K

K

G

G

A

A

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I

A

A

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K

A

A

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E

L

I

V

L

A

A

A

K

T

T

P

P

G

N

A

G

W

Y

M

M

P

L

Q

Q

Q
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Q

F

F

E

E

N

N

P

P

A

A

N

N

I

P

E

K

A

I

H

H

V

Y

N

E

T

T

A

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E

E

I

I

L

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A

K

D

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T

P

G

Q

G

G

K

I

I

D

D

A

G

L

F

V

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T

S

G
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G

V
x
I

G
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G

T
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T

G
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G

H
x
T

I

I

T

T

G

G

C

A

A

G

R

K

V

Y

L

L

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K

Q

E

K

Q

W

N

P

A

A

N

L

V

K

K

V

L

Y

Y

A

G

V

V

E

E

P

P

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V

A

E

S

S

P

A

V

I

I

L

S

S

G

G

A

K

P

P

A

G

P

P

H

H

P

K

I

I

Q
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Q

G
|
G

I
|
I

G

G

A

A

G

G

F

F

I

I

P

P

R

S

N

V

L

L

D

N

T

V

A

D

L

L

L

I

D

D

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E

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V

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E

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M

M

A

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A

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A

A

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L

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K

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

T

A

L

A

A

A

I

A

A

I

Q

-

K

K

L

L

P

A

D

Q

L

R

P

P

-

E

-

N

A

A

G

G

A

K

T

L

V

F

L

V

G

A

F

I

N

F

Y
x
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

T

S

V

T

E

V

G

L

F

F

active site: A44 (≠ K42), S267 (= S264)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: T72 (= T69), G74 (= G71), N75 (= N72), T76 (= T73), Q145 (= Q142), G177 (= G174), I178 (≠ V175), G179 (= G176), T180 (= T177), G181 (= G178), G182 (= G179), T183 (≠ H180), Q222 (= Q219), G223 (= G220), I224 (= I221), S267 (= S264), P294 (≠ Y290), S295 (≠ D291)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
49% identity, 98% coverage: 3:302/305 of query aligns to 14:316/323 of 4aecA

query
sites
4aecA

A

A

D

D

N

N

I

V

L

S

Q

Q

T

L

I

I

G

G

N

K

T

T

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P

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M

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Y

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N

R

S

L

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F

A

-

K

G

G

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H

A

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N

V

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A

I

A

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K

S

L

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M

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E

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P

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C

S

S

I
x
V

K
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K

D

D

R

R

I

I

A

G

L

Y

S

S

M

M

V

V

E

T

A

D

A

A

E

E

Q

Q

S

K

G

G

V

F

L

I

K

S

P

P

G

G

G

K

T

S

I

V

-

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

M

F

V

I

A

A

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S

K

R

G

G

Y

Y

K

R

L

L

V

I

L

L

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T

M

M

P

P

D

A

S

S

M

M

S

S

V

M

E

E

R

R

R

V

L

L

M

L

L

K

A

A

Y

F

G

G

A

A

T

E

F

L

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V

L

L

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T

P

D

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P

E

A

K

K

G

G

M

M

K

T

G

G

A

A

I

V

A

Q

R

K

A

A

E

E

L

I

V

L

A

K

A

N

T

T

P

P

G

D

A

A

W

Y

M

M

P

L

Q

Q

F

F

E

D

N

N

P

P

A

A

N

N

I

P

E

K

A

I

H

H

V

Y

N

E

T

T

A

G

L

P

E

E

I

I

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W

A

D

D

D

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T

P

K

Q

G

G

K

I

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D

D

A

I

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V

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T

A

G
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G

V
x
I

G
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G

T
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T

G
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G

H
x
T

I

I

T

T

G

G

C

V

A

G

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R

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F

L

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E

K

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N

P

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A

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V

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S

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S

G

G

A

K

P

P

A

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H

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K

I

I

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Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

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K

N

N

L

L

D

D

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A

K

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M

D

D

G

E

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V

I

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Q

A

V

I

D

S

A

S

E

E

P

E

A

A

R

I

E

E

M

T

A

A

R

K

R

Q

A

L

A

A

R

L

E

K

E

E

G

G

M

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

T

A

L

A

A

A

I

A

A

I

Q

K

-

V

-

A

K

K

L

R

P

P

D

E

L

-

P

N

A

A

G

G

A

K

T

L

V

I

L

A

G

V

F

V

N

F

Y
x
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

T

S

V

T

E

P

G

L

F

F

active site: K54 (= K42), S277 (= S264)
binding pyridoxal-5'-phosphate: V53 (≠ I41), K54 (= K42), N85 (= N72), G187 (= G174), I188 (≠ V175), G189 (= G176), T190 (= T177), G191 (= G178), G192 (= G179), T193 (≠ H180), Q232 (= Q219), G233 (= G220), S277 (= S264), P304 (≠ Y290), S305 (≠ D291)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
51% identity, 95% coverage: 10:298/305 of query aligns to 7:297/302 of 2efyA

query
sites
2efyA

I

I

G

G

N

K

T

T

P

P

H

V

I

V

R

R

V

L

Q

A

R

K

L

V

F

V

G

E

-

P

D

D

T

M

H

A

Q

E

V

V

W

W

I

V

K

K

S

L

E

E

R

G

A

L

N

N

P

P

G

G

S

S

I

I

K
|
K

D

D

R

R

I

P

A

A

L

W

S

Y

M

M

V

I

E

K

A

D

A

A

E

E

Q

E

S

R

G

G

V

I

L

L

K

R

P

P

G

G

G

S

-

G

-

Q

T

V

I

I

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

M

V

I

A

A

V

S

K

R

G

G

Y

Y

K

R

L

L

V

I

L

L

V

T

M

M

P

P

D

A

S

Q

M

M

S

S

V

E

E

E

R

R

R

K

R

R

L

V

M

L

L

K

A

A

Y

F

G

G

A

A

T

E

F

L

D

V

L

L

T

T

P

D

R

P

E

E

K

R

G

R

M

M

K

L

G

A

A

A

I

R

A

E

R

E

A

A

E

L

E

R

L

L

V

K

A

E

T

L

P

-

G

G

A

A

W

F

M

M

P

P

Q

D

Q
|
Q

F

F

E

K

N

N

P

P

A

A

N

N

I

V

E

R

A

A

H

H

V

Y

N

E

T

T

A

G

L

P

E

E

I

L

L

Y

A

E

D

A

F

L

P

E

Q

G

G

R

I

I

D

D

A

A

L

F

V

V

T
x
Y

G
|
G

V
x
S

G
|
G

T
|
T

G
|
G

H
x
T

I

I

T

T

G

G

C

V

A

G

R

R

V

Y

L

L

K

K

Q

E

K

R

W

I

P

P

A

H

L

V

K

K

V

V

Y

I

A

A

V

V

E
|
E

P

P

V

A

A

R

S
|
S

P

N

V

V

I

L

S

S

G

G

A

K

P

M

A

G

P

Q

H

H

P

G

I

F

Q
|
Q

G
|
G

I
x
M

G
|
G

A
x
P

G

G

F

F

I

I

P

P

R

E

N

N

L

L

D

D

T

L

A

S

L

L

D

D

G

G

V

V

I

I

Q

Q

V

V

D

W

A

E

E

E

P

D

A

A

R

F

E

P

M

L

A

A

R

R

A

L

A

A

R

R

E

E

G

G

M

L

L

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

T

I

L

V

A

W

A

A

I

A

A

L

Q

Q

K

V

L

A

P

R

D

E

L

L

P

G

A

P

G

G

A

K

T

R

V

V

L

A

G

C

F

I

N

S

Y
x
P

D
|
D

T

G

G

G

E

W

R

K

Y

Y

L

L

T

S

active site: K40 (= K42), S70 (= S70), E200 (= E201), S204 (= S205), S263 (= S264)
binding 5-oxohexanoic acid: K40 (= K42), T69 (= T69), G71 (= G71), N72 (= N72), T73 (= T73), Q141 (= Q142), G175 (= G176), G219 (= G220), M220 (≠ I221), G221 (= G222), P222 (≠ A223)
binding pyridoxal-5'-phosphate: K40 (= K42), N72 (= N72), Y172 (≠ T173), G173 (= G174), S174 (≠ V175), G175 (= G176), T176 (= T177), G177 (= G178), G178 (= G179), T179 (≠ H180), Q218 (= Q219), G219 (= G220), M220 (≠ I221), S263 (= S264), P289 (≠ Y290), D290 (= D291)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
51% identity, 95% coverage: 10:298/305 of query aligns to 7:297/302 of 2ecqA

query
sites
2ecqA

I

I

G

G

N

K

T

T

P

P

H

V

I

V

R

R

V

L

Q

A

R

K

L

V

F

V

G

E

-

P

D

D

T

M

H

A

Q

E

V

V

W

W

I

V

K

K

S

L

E

E

R

G

A

L

N

N

P

P

G

G

S

S

I

I

K
|
K

D

D

R

R

I

P

A

A

L

W

S

Y

M

M

V

I

E

K

A

D

A

A

E

E

Q

E

S

R

G

G

V

I

L

L

K

R

P

P

G

G

G

S

-

G

-

Q

T

V

I

I

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

M

V

I

A

A

V

S

K

R

G

G

Y

Y

K

R

L

L

V

I

L

L

V

T

M

M

P

P

D

A

S

Q

M

M

S

S

V

E

E

E

R

R

R

K

R

R

L

V

M

L

L

K

A

A

Y

F

G

G

A

A

T

E

F

L

D

V

L

L

T

T

P

D

R

P

E

E

K

R

G

R

M

M

K

L

G

A

A

A

I

R

A

E

R

E

A

A

E

L

E

R

L

L

V

K

A

E

T

L

P

-

G

G

A

A

W

F

M

M

P

P

Q

D

Q
|
Q

F

F

E

K

N

N

P

P

A

A

N

N

I

V

E

R

A

A

H

H

V

Y

N

E

T

T

A

G

L

P

E

E

I

L

L

Y

A

E

D

A

F

L

P

E

Q

G

G

R

I

I

D

D

A

A

L

F

V

V

T
x
Y

G
|
G

V
x
S

G
|
G

T
|
T

G
|
G

H
x
T

I

I

T

T

G

G

C

V

A

G

R

R

V

Y

L

L

K

K

Q

E

K

R

W

I

P

P

A

H

L

V

K

K

V

V

Y

I

A

A

V

V

E
|
E

P

P

V

A

A

R

S
|
S

P

N

V

V

I

L

S

S

G

G

A

K

P

M

A

G

P

Q

H

H

P

G

I

F

Q
|
Q

G
|
G

I
x
M

G

G

A

P

G

G

F

F

I

I

P

P

R

E

N

N

L

L

D

D

T

L

A

S

L

L

D

D

G

G

V

V

I

I

Q

Q

V

V

D

W

A

E

E

E

P

D

A

A

R

F

E

P

M

L

A

A

R

R

A

L

A

A

R

R