SitesBLAST

3ws7A The 1.18 a resolution structure of l-serine 3-dehydrogenase complexed with NADP+ and sulfate ion from the hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
38% identity, 91% coverage: 1:260/286 of query aligns to 24:281/293 of 3ws7A

query
sites
3ws7A

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binding nadp nicotinamide-adenine-dinucleotide phosphate: M24 (= M1), N43 (≠ S20), R44 (= R21), T45 (≠ S22), K48 (≠ P25), M76 (= M52), V77 (= V53), S78 (≠ P54), D82 (≠ H58), Q85 (≠ A61), V86 (≠ A62), S107 (= S83), T108 (≠ S84), V133 (= V109), G136 (= G112), K183 (= K159), G240 (= G219), F241 (= F220), K242 (≠ R221), H245 (≠ L224), K248 (= K227), D249 (= D228)
binding sulfate ion: T134 (≠ S110), G135 (= G111), G136 (= G112), K183 (= K159), N186 (= N162), V190 (= V166)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 20, 21, 22, 23

5je8B The crystal structure of bacillus cereus 3-hydroxyisobutyrate dehydrogenase in complex with NAD (see paper)
29% identity, 95% coverage: 1:273/286 of query aligns to 14:289/294 of 5je8B

query
sites
5je8B

M
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A

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binding nicotinamide-adenine-dinucleotide: M14 (= M1), D33 (≠ S19), L34 (≠ S20), S66 (≠ M52), L67 (≠ V53), P68 (= P54), V76 (≠ A62), S97 (= S83), T98 (≠ S84), V123 (= V109), G126 (= G112), L240 (= L224), Q274 (≠ S258), A275 (≠ H259)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 11, 12, 13

3pefA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter metallireducens in complex with NADP+ (see paper)
31% identity, 96% coverage: 1:274/286 of query aligns to 12:286/287 of 3pefA

query
sites
3pefA

M
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M

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E

binding glycerol: D67 (= D55), G123 (= G111), S124 (≠ G112), K171 (= K159), N175 (≠ Q163), M178 (≠ V166), L203 (≠ R191), G207 (≠ M195), A212 (≠ S200), N213 (≠ S201), F216 (≠ L204), A217 (≠ E205), F232 (= F220), K235 (≠ G223), H236 (≠ L224), K239 (= K227), R242 (≠ N230), R269 (≠ A257)
binding nadp nicotinamide-adenine-dinucleotide phosphate: M12 (= M1), N31 (≠ S20), R32 (= R21), S33 (= S22), K36 (≠ P25), M64 (= M52), L65 (≠ V53), A66 (≠ P54), A70 (≠ H58), E73 (≠ A61), V74 (≠ A62), S95 (= S83), T96 (≠ S84), V121 (= V109), G123 (= G111), S124 (≠ G112), K171 (= K159), A231 (≠ G219), F232 (= F220), P233 (≠ R221), H236 (≠ L224), K239 (= K227)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 8, 9, 10, 11

P29266 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Rattus norvegicus (Rat) (see paper)
29% identity, 96% coverage: 1:274/286 of query aligns to 49:330/335 of P29266

query
sites
P29266

M

M

G

G

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M

A

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N

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L

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R

D68 (≠ S20) mutation to R: Decrease of activity with NAD, increase of activity with NADP.
K208 (= K159) mutation K->A,H,N,R: Complete loss of activity.
N212 (≠ Q163) mutation to Q: Decrease in activity.

3pduA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter sulfurreducens in complex with NADP+ (see paper)
29% identity, 96% coverage: 1:274/286 of query aligns to 12:286/287 of 3pduA

query
sites
3pduA

M
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M

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G

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P

M

M

A

A

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G

G

A

V

R

A

Q

C

A

S

S

G

P

K

A

E

E

V

V

A

C

E

A

R

A

A

C

D

D

V

I

I

T

F

I

L

A

M
|
M

V
x
L

P
x
A

D

D

T

P

P

A

H
x
A

V

A

E

R

A
x
E

A
x
V

L

C

F

F

N

G

N

A

G

N

G

G

I

V

A

L

A

E

G

G

L

I

S

G

A

G

G

G

K

R

I

G

V

Y

V

I

D

D

M

M

S
|
S

S
x
T

I

V

S

D

P

D

I

E

A

T

T

S

K

T

A

A

F

I

A

G

E

A

R

A

V

V

N

T

A

A

L

R

G

G

C

G

Q

R

Y

F

L

L

D

E

A

A

P

P

V
|
V

S

S

G
|
G

G
x
T

E

K

V

K

G

P

A

A

R

E

N

D

A

G

T

T

L

L

S

I

I

I

M

L

V

A

G

A

G

G

P

D

Q

Q

A

S

T

L

F

F

E

T

T

D

V

A

R

G

P

P

L

A

F

F

E

A

L

A

M

L

G

G

K

K

N

K

I

C

T

L

L

H

V

L

G

G

D

E

N

V

G

G

A

Q

G

G

Q

A

T

R

A

M

K
|
K

V

L

A

V

N

V

Q

N

I

M

I

I

V

M

A

G

L

Q

N

M

I

M

E

T

A

A

V

L

A

G

E

E

A

G

L

M

L

A

F

L

A

G

S

R

K

N

A

C

G

G

A

L

D

D

P

G

A

G

R

Q

V

L

R

L

Q

E

A

V

L

L

M

D

G

A

G

G

F

A

A

M

S

A

S

N

K

P

V

M

L

F

E

K

V

G

H

K

G

G

E

Q

R

M

M

L

I

L

K

S

R

G

T

E

F

F

D

P

P

T

G
x
S

F
|
F

R
x
P

I

L

G

K

L
x
H

H

M

Q

Q

K
|
K

D
|
D

L

L

N
x
R

L

L

A

A

L

V

S
x
E

N

L

A

G

R
x
D

E
x
R

M

L

G

G

V

Q

S

P

L

L

P

H

N

G

T

A

A

A

T

T

C

A

Q

N

E

E

L

S

F

F

N

K

A

R

C

A

A

R

S

A

H

A

G

G

G

H

S

A

D

D

W

E

D

D

H

F

S

A

A

A

L

V

V

F

R

R

A

V

L

L

E

E

binding glycerol: R242 (≠ N230), E246 (≠ S234), E246 (≠ S234), D249 (≠ R237), R250 (≠ E238)
binding nadp nicotinamide-adenine-dinucleotide phosphate: M12 (= M1), N31 (≠ S20), R32 (= R21), N33 (≠ S22), M64 (= M52), L65 (≠ V53), A66 (≠ P54), A70 (≠ H58), E73 (≠ A61), V74 (≠ A62), S95 (= S83), T96 (≠ S84), V121 (= V109), G123 (= G111), T124 (≠ G112), K171 (= K159), S231 (≠ G219), F232 (= F220), P233 (≠ R221), H236 (≠ L224), K239 (= K227), D240 (= D228)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 8, 9, 10, 11

P31937 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Homo sapiens (Human) (see paper)
29% identity, 96% coverage: 1:274/286 of query aligns to 50:331/336 of P31937

query
sites
P31937

M
|
M

G
|
G

T
x
N

P
|
P

M
|
M

A
|
A

A
x
K

N
|
N

L
|
L

I
x
M

A
x
K

G
x
H

G
|
G

H
x
Y

E
x
P

V
x
L

S
x
I

L
x
I

S
x
Y

S

D

-

V

R

F

S

P

G

D

V

A

P

C

Q

K

S

E

L

F

L

Q

D

D

S

A

G

G

G

E

V

Q

A

V

C

V

S

S

G

P

K

A

E

D

V

V

A

A

E

E

R

K

A

A

D

D

V

R

I

I

F

I

L

T

M

M

V
x
L

P
|
P

D

T

T

S

P

I

H
x
N

V

A

E

I

A

E

A

A

L

Y

F

S

N

G

N

A

G

N

G

G

I

I

A

L

A

K

G

K

L

V

S

K

A

K

G

G

K

S

I

L

V

L

V

I

D

D

M

S

S

S

S
x
T

I

I

S

D

P

P

I

A

A

V

T

S

K

K

A

E

F

L

A

A

E

K

R

E

V

V

N

E

A

K

L

M

G

G

C

A

Q

V

Y

F

L

M

D

D

A

A

P

P

V

V

S

S

G

G

E

V

V

G

G

A

A

A

R

R

N

S

A

G

T

N

L

L

S

T

I

F

M

M

V

V

G

G

P

V

Q

E

A

D

T

E

F

F

E

A

T

A

V

A

R

Q

P

E

L

L

F

L

E

G

L

C

M

M

G

G

K

S

N

N

I

V

T

V

L

Y

V

C

G

G

D

A

N

V

G

G

A

T

G

G

Q

Q

T

A

A

A

K

K

V

I

A

C

N

N

Q

N

I

M

I

L

V

L

A

A

L

I

N

S

I

M

E

I

A

G

V

T

A

A

E

E

A

A

L

M

L

N

F

L

A

G

S

I

K

R

A

L

G

G

A

L

D

D

P

P

A

K

R

L

V

L

R

-

Q

-

A

-

L

-

M

-

G

-

F

-

A

-

S

-

S

A

K

K

V

I

L

L

E

N

V

M

H

S

G

S

E

G

R

R

M

C

I

W

K

S

R

S

-

D

T

T

F

Y

D

N

P

P

-

V

-

P

-

G

-

V

-

M

-

D

-

G

-

V

-

P

-

S

-

A

-

N

-

Y

-

Q

-

G

G

G

F

F

R

G

I

T

G

T

L

L

H

M

Q

A

K
|
K

D

D

L

L

N

G

L

L

A

A

L

Q

S

D

N

S

A

A

R

T

E

S

M

T

G

K

V

S

S

P

L

I

P

L

N

L

T

G

A

S

T

L

C

A

Q

H

E

Q

L

I

F

Y

N

R

A

M

C

M

A

C

S

A

H

K

G

G

G

Y

S

S

D

K

W

K

D

D

H

F

S

S

A

S

L

V

V

F

R

Q

A

F

L

L

E

R

LP 103:104 (≠ VP 53:54) binding
N108 (≠ H58) binding
T134 (≠ S84) binding
K284 (= K227) binding

Sites not aligning to the query:

1:36 modified: transit peptide, Mitochondrion
40:68 binding

2i9pB Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+
29% identity, 96% coverage: 1:274/286 of query aligns to 11:292/296 of 2i9pB

query
sites
2i9pB

M
|
M

G

G

T

N

P

P

M

M

A

A

A

K

N

N

L

L

I

M

A

K

G

H

G

G

H

Y

E

P

V

L

S

I

L

I

S
x
Y

S
x
D

-
x
V

R
x
F

S

P

G

D

V

A

P

C

Q

K

S

E

L

F

L

Q

D

D

S

A

G

G

G

E

V

Q

A

V

C

V

S

S

G

P

K

A

E

D

V

V

A

A

E

E

R

K

A

A

D

D

V

R

I

I

F

I

L

T

M
|
M

V
x
L

P
|
P

D

T

T

S

P

I

H
x
N

V

A

E

I

A

E

A
|
A

L

Y

F

S

N

G

N

A

G

N

G

G

I

I

A

L

A

K

G

K

L

V

S

K

A

K

G

G

K

S

I

L

V

L

V

I

D

D

M

S

S
|
S

S
x
T

I

I

S

D

P

P

I

A

A

V

T

S

K

K

A

E

F

L

A

A

E

K

R

E

V

V

N

E

A

K

L

M

G

G

C

A

Q

V

Y

F

L

M

D

D

A

A

P

P

V
|
V

S

S

G
|
G

E

V

V

G

G

A

A

A

R

R

N

S

A

G

T

N

L

L

S

T

I

F

M

M

V

V

G

G

P

V

Q

E

A

D

T

E

F

F

E

A

T

A

V

A

R

Q

P

E

L

L

F

L

E

G

L

C

M

M

G

G

K

S

N

N

I

V

T

V

L

Y

V

C

G

G

D

A

N

V

G

G

A

T

G

G

Q

Q

T

A

A

A

K
|
K

V

I

A

C

N

N

Q

N

I

M

I

L

V

L

A

A

L

I

N

S

I

M

E

I

A

G

V

T

A

A

E

E

A

A

L

M

L

N

F

L

A

G

S

I

K

R

A

L

G

G

A

L

D

D

P

P

A

K

R

L

V

L

R

-

Q

-

A

-

L

-

M

-

G

-

F

-

A

-

S

-

S

A

K

K

V

I

L

L

E

N

V

M

H

S

G

S

E

G

R

R

M

C

I

W

K

S

R

S

-

D

T

T

F

Y

D

N

P

P

-

V

-

P

-

G

-

V

-

M

-

D

-

G

-

V

-

P

-

S

-

A

-

N

-

Y

-

Q

-

G

G

G

F
|
F

R

G

I

T

G

T

L
|
L

H

M

Q

A

K
|
K

D
|
D

L

L

N

G

L

L

A

A

L

Q

S

D

N

S

A

A

R

T

E

S

M

T

G

K

V

S

S

P

L

I

P

L

N

L

T

G

A

S

T

L

C

A

Q

H

E

Q

L

I

F

Y

N

R

A

M

C

M

A

C

S

A

H

K

G

G

G

Y

S

S

D

K

W

K

D

D

H

F

S

S

A

S

L

V

V

F

R

Q

A

F

L

L

E

R

binding nicotinamide-adenine-dinucleotide: M11 (= M1), Y29 (≠ S19), D30 (≠ S20), V31 (vs. gap), F32 (≠ R21), M63 (= M52), L64 (≠ V53), P65 (= P54), N69 (≠ H58), A73 (= A62), S94 (= S83), T95 (≠ S84), V120 (= V109), G122 (= G111), G123 (= G112), K170 (= K159), F238 (= F220), L242 (= L224), K245 (= K227), D246 (= D228)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 6, 7, 8, 9, 10

1wp4A Structure of tt368 protein from thermus thermophilus hb8 (see paper)
34% identity, 85% coverage: 40:281/286 of query aligns to 50:287/288 of 1wp4A

query
sites
1wp4A

K

E

E

R

V

V

A

A

E

E

R

-

A

A

D

R

V

V

I

I

F

F

L

T

M
x
C

V
x
L

P
|
P

D

T

T

T

P

R

H
x
E

V

V

E

Y

A
x
E

-

V

-

A

-

E

A

A

L

L

F

Y

N

P

N

Y

G

-

I

-

A

-

G

-

L

L

S

R

A

E

G

G

K

T

I

Y

V

W

V

V

D

D

M

A

S
x
T

S
|
S

I

G

S

E

P

P

I

E

A

A

T

S

K

R

A

R

F

L

A

A

E

E

R

R

V

L

N

R

A

E

L

K

G

G

C

V

Q

T

Y

Y

L

L

D

D

A

A

P

P

V
|
V

S
|
S

G
|
G

E
x
T

V

S

G

G

A

A

R

E

N

A

A

G

T

T

L

L

S

T

I

V

M

M

V

L

G

G

P

P

Q

E

A

E

T

A

F

V

E

E

T

R

V

V

R

R

P

P

L

-

F

F

E

L

L

A

M

Y

G

A

K

K

N

K

I

V

T

V

L

H

V

V

G

G

D

P

N

V

G

G

A

A

G

G

Q

H

T

A

A

V

K
|
K

V

A

A

I

N
|
N

Q
x
N

I

A

I

L

V

L

A

A

L

V

N

N

I

L

E

W

A

A

V

A

A

G

E

E

A

G

L

L

F

A

A

L

S

V

K

K

A

Q

G

G

A

V

D

S

P

A

A

E

R

K

V

A

R

L

Q

E

A

V

L

I

M

N

G

A

G

S

F

S

A

G

S

R

S

S

K

N

V

A

L

T

E

E

-

N

V

L

H

I

G

P

E

Q

R

R

M

V

I

L

K

T

R

R

T

A

F

F

D

P

P

K

G
x
T

F
|
F

R

A

I

L

G

G

L
|
L

H

L

Q

V

K
|
K

D
|
D

L

L

N

G

L

I

A

A

L

M

S

G

N

V

A

L

R

D

E

G

M

E

G

K

V

A

S

P

L

S

P

P

N

L

T

L

A

R

T

L

C

A

Q

R

E

E

L

V

F

Y

N

E

A

M

C

A

A

K

S

R

H

E

G

L

G

G

S

P

D

D

W

A

D

D

H

H

S

V

A

E

L

A

V

L

R

R

A

L

L

L

E

E

L

R

M

W

A

G

N

G

H

V

E

E

V

I

active site: S116 (= S110), K164 (= K159), N167 (= N162), N168 (≠ Q163)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: C61 (≠ M52), L62 (≠ V53), P63 (= P54), E67 (≠ H58), E70 (≠ A61), T89 (≠ S83), S90 (= S84), V115 (= V109), G118 (= G112), T119 (≠ E113), K164 (= K159), T225 (≠ G219), F226 (= F220), L230 (= L224), K233 (= K227), D234 (= D228)
binding sulfate ion: S116 (= S110), G117 (= G111), G118 (= G112), K164 (= K159), N168 (≠ Q163), F226 (= F220)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 7, 8, 9, 10, 11, 28, 29, 30, 31, 34

2cvzC Structure of hydroxyisobutyrate dehydrogenase from thermus thermophilus hb8 (see paper)
34% identity, 85% coverage: 40:281/286 of query aligns to 51:288/289 of 2cvzC

query
sites
2cvzC

K

E

E

R

V

V

A

A

E

E

R

-

A

A

D

R

V

V

I

I

F

F

L

T

M
x
C

V
x
L

P
|
P

D

T

T

T

P

R

H
x
E

V

V

E

Y

A
x
E

-

V

-

A

-

E

A

A

L

L

F

Y

N

P

N

Y

G

-

I

-

A

-

G

-

L

L

S

R

A

E

G

G

K

T

I

Y

V

W

V

V

D

D

M

A

S
x
T

S
|
S

I

G

S

E

P

P

I

E

A

A

T

S

K

R

A

R

F

L

A

A

E

E

R

R

V

L

N

R

A

E

L

K

G

G

C

V

Q

T

Y

Y

L

L

D

D

A

A

P

P

V
|
V

S
|
S

G

G

G
|
G

E

T

V

S

G

G

A

A

R

E

N

A

A

G

T

T

L

L

S

T

I

V

M

M

V

L

G

G

P

P

Q

E

A

E

T

A

F

V

E

E

T

R

V

V

R

R

P

P

L

-

F

F

E

L

L

A

M

Y

G

A

K

K

N

K

I

V

T

V

L

H

V

V

G

G

D

P

N

V

G

G

A

A

G

G

Q

H

T

A

A

V

K
|
K

V

A

A

I

N
|
N

Q
x
N

I

A

I

L

V

L

A

A

L

V

N

N

I

L

E

W

A

A

V

A

A

G

E

E

A

G

L

L

F

A

A

L

S

V

K

K

A

Q

G

G

A

V

D

S

P

A

A

E

R

K

V

A

R

L

Q

E

A

V

L

I

M

N

G

A

G

S

F

S

A

G

S

R

S

S

K

N

V

A

L

T

E

E

-

N

V

L

H

I

G

P

E

Q

R

R

M

V

I

L

K

T

R

R

T

A

F

F

D

P

P

K

G
x
T

F
|
F

R

A

I

L

G

G

L
|
L

H

L

Q

V

K
|
K

D
|
D

L

L

N

G

L

I

A

A

L

M

S

G

N

V

A

L

R

D

E

G

M

E

G

K

V

A

S

P

L

S

P

P

N

L

T

L

A

R

T

L

C

A

Q

R

E

E

L

V

F

Y

N

E

A

M

C

A

A

K

S

R

H

E

G

L

G

G

S

P

D

D

W

A

D

D

H

H

S

V

A

E

L

A

V

L

R

R

A

L

L

L

E

E

L

R

M

W

A

G

N

G

H

V

E

E

V

I

active site: S117 (= S110), K165 (= K159), N168 (= N162), N169 (≠ Q163)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: C62 (≠ M52), L63 (≠ V53), P64 (= P54), E68 (≠ H58), E71 (≠ A61), T90 (≠ S83), S91 (= S84), V116 (= V109), G119 (= G112), K165 (= K159), T226 (≠ G219), F227 (= F220), L231 (= L224), K234 (= K227), D235 (= D228)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 8, 9, 10, 11, 12, 29, 30, 31, 32

6smzC Crystal structure of sla reductase yihu from e. Coli in complex with nadh
29% identity, 97% coverage: 1:277/286 of query aligns to 11:289/295 of 6smzC

query
sites
6smzC

M
|
M

G

G

T

S

P

P

M

M

A

A

A

S

N

N

L

L

I

L

A

Q

G

Q

G

G

H

H

E

Q

V

L

S

R

L

V

-
x
F

S
x
D

S
x
V

R
x
N

S

A

G

E

V

A

P

V

Q

R

S

H

L

L

L

V

D

D

S

K

G

G

G

A

V

T

A

P

C

A

S

A

S

N

G

P

K

A

E

Q

V

A

A

A

E

K

R

D

A

A

D

E

V

F

I

I

F

I

L

T

M
|
M

V
x
L

P
|
P

D

N

T

G

P

D

H
x
L

V

V

E

R

A

N

A
x
V

L

L

F

F

N

G

N

E

G

N

G

G

I

V

A

C

A

E

G

G

L

L

S

S

A

T

G

D

K

A

I

L

V

V

V

I

D

D

M

M

S
|
S

S
x
T

I

I

S

H

P

P

I

L

A

Q

T

T

K

D

A

K

F

L

A

I

E

A

R

D

V

M

N

Q

A

A

L

K

G

G

C

F

Q

S

Y

M

L

M

D

D

A

V

P

P

V
|
V

S

G

G
x
R

G

T

E

S

V

A

G

N

A

A

R

I

N

T

A

G

T

T

L

L

S

L

I

L

M

L

V

A

G

G

P

T

Q

A

A

E

T

Q

F

V

E

E

T

R

V

A

R

T

P

P

L

I

F

L

E

M

L

A

M

M

G

G

K

S

N

E

I

L

T

I

L

N

V

A

G

G

D

G

N

P

G

G

A

M

G

G

Q

I

T

R

A

V

K

K

V

L

A

I

N

N

Q

N

I

Y

I

M

-

S

V

I

A

A

L

L

N

N

I

A

E

L

A

S

V

A

A

E

E

A

A

A

L

V

L

L

F

-

A

-

S

C

K

E

A

A

G

L

A

N

D

L

P

P

A

F

R

D

V

V

R

A

Q

V

A

K

L

V

M

M

G

S

G

G

F

T

A

A

S

A

S

G

K

K

-

G

-

H

V

F

L

T

E

T

V

S

H

W

G

P

E

N

R

K

M

V

I

L

K

S

R

G

T

D

F

L

D

S

P

P

G

A

F

F

R

M

I

I

G

D

L

L

H

A

Q

H

K

K

D

D

L

L

N

G

L

I

A

A

L

L

S

D

N

V

A

A

R

N

E

Q

M

L

G

H

V

V

S

P

L

M

P

P

N

L

T

G

A

A

T

A

C

S

Q

R

E

E

L

V

F

Y

N

S

A

Q

C

A

A

R

S

A

H

A

G

G

G

R

S

G

D

R

W

Q

D

D

H

W

S

S

A

A

L

I

V

L

R

E

A

Q

L

V

E

R

L

V

M

S

A

A

binding nicotinamide-adenine-dinucleotide: M11 (= M1), F29 (vs. gap), D30 (≠ S19), V31 (≠ S20), N32 (≠ R21), M63 (= M52), L64 (≠ V53), P65 (= P54), L69 (≠ H58), V73 (≠ A62), S94 (= S83), T95 (≠ S84), V120 (= V109), R122 (≠ G111)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 7, 8, 9, 10

6smyA Crystal structure of sla reductase yihu from e. Coli with nadh and product dhps
29% identity, 97% coverage: 1:277/286 of query aligns to 11:289/294 of 6smyA

query
sites
6smyA

M

M

G

G

T

S

P

P

M

M

A

A

A

S

N

N

L

L

I

L

A

Q

G

Q

G

G

H

H

E

Q

V

L

S

R

L

V

-

F

S

D

S

V

R

N

S

A

G

E

V

A

P

V

Q

R

S

H

L

L

L

V

D

D

S

K

G

G

G

A

V

T

A

P

C

A

S

A

S

N

G

P

K

A

E

Q

V

A

A

A

E

K

R

D

A

A

D

E

V

F

I

I

F

I

L

T

M

M

V

L

P

P

D

N

T

G

P

D

H

L

V

V

E

R

A

N

A

V

L

L

F

F

N

G

N

E

G

N

G

G

I

V

A

C

A

E

G

G

L

L

S

S

A

T

G

D

K

A

I

L

V

V

V

I

D

D

M

M

S

S

S

T

I

I

S

H

P

P

I

L

A

Q

T

T

K

D

A

K

F

L

A

I

E

A

R

D

V

M

N

Q

A

A

L

K

G

G

C

F

Q

S

Y

M

L

M

D

D

A

V

P

P

V

V

S

G

G

R

G

T

E

S

V

A

G

N

A

A

R

I

N

T

A

G

T

T

L

L

S

L

I

L

M

L

V

A

G

G

P

T

Q

A

A

E

T

Q

F

V

E

E

T

R

V

A

R

T

P

P

L

I

F

L

E

M

L

A

M

M

G

G

K

S

N

E

I

L

T

I

L

N

V

A

G

G

D

G

N

P

G

G

A

M

G

G

Q

I

T

R

A

V

K

K

V

L

A

I

N

N

Q

N

I

Y

I

M

-

S

V

I

A

A

L

L

N

N

I

A

E

L

A

S

V

A

A

E

E

A

A

A

L

V

L

L

F

-

A

-

S

C

K

E

A

A

G

L

A

N

D

L

P

P

A

F

R

D

V

V

R

A

Q

V

A

K

L

V

M

M

G

S

G

G

F
x
T

A

A

S

A

S

G

K

K

-

G

-

H

V

F

L

T

E

T

V

S

H

W

G

P

E

N

R

K

M

V

I

L

K

S

R

G

T

D

F

L

D

S

P

P

G

A

F
|
F

R

M

I

I

G

D

L

L

H

A

Q

H

K

K

D

D

L

L

N

G

L

I

A

A

L

L

S

D

N

V

A

A

R

N

E

Q

M

L

G

H

V

V

S

P

L

M

P

P

N

L

T

G

A

A

T

A

C

S

Q

R

E

E

L

V

F

Y

N

S

A

Q

C

A

A

R

S

A

H

A

G

G

G

R

S

G

D

R

W

Q

D

D

H
x
W

S

S

A

A

L

I

V

L

R

E

A

Q

L

V

E

R

L

V

M

S

A

A

binding (2~{S})-2,3-bis(oxidanyl)propane-1-sulfonic acid: T208 (≠ F198), F232 (= F220), W278 (≠ H266)

P0A9V8 3-sulfolactaldehyde reductase; SLA reductase; 4-hydroxybutyrate dehydrogenase; Gamma-hydroxybutyrate dehydrogenase; GHBDH; Succinic semialdehyde reductase; SSA reductase; EC 1.1.1.373; EC 1.1.1.61 from Escherichia coli (strain K12)
29% identity, 97% coverage: 1:277/286 of query aligns to 12:290/298 of P0A9V8

query
sites
P0A9V8

M
|
M

G

G

T

S

P

P

M

M

A

A

A

S

N

N

L

L

I

L

A

Q

G

Q

G

G

H

H

E

Q

V

L

S

R

L

V

-

F

S
x
D

S

V

R

N

S

A

G

E

V

A

P

V

Q

R

S

H

L

L

L

V

D

D

S

K

G

G

G

A

V

T

A

P

C

A

S

A

S

N

G

P

K

A

E

Q

V

A

A

A

E

K

R

D

A

A

D

E

V

F

I

I

F

I

L

T

M

M

V
x
L

P

P

D

N

T

G

P

D

H

L

V

V

E

R

A

N

A

V

L

L

F

F

N

G

N

E

G

N

G

G

I

V

A

C

A

E

G

G

L

L

S

S

A

T

G

D

K

A

I

L

V

V

V

I

D

D

M

M

S

S

S
x
T

I

I

S

H

P

P

I

L

A

Q

T

T

K

D

A

K

F

L

A

I

E

A

R

D

V

M

N

Q

A

A

L

K

G

G

C

F

Q

S

Y

M

L

M

D

D

A

V

P

P

V

V

S
x
G

G
x
R

G
x
T

E

S

V

A

G

N

A

A

R

I

N

T

A

G

T

T

L

L

S

L

I

L

M

L

V

A

G

G

P

T

Q

A

A

E

T

Q

F

V

E

E

T

R

V

A

R

T

P

P

L

I

F

L

E

M

L

A

M

M

G

G

K

S

N

E

I

L

T

I

L

N

V

A

G

G

D

G

N

P

G

G

A

M

G

G

Q

I

T

R

A

V

K

K

V

L

A

I

N
|
N

Q
x
N

I
x
Y

I
x
M

-
x
S

V

I

A

A

L

L

N

N

I

A

E

L

A

S

V

A

A

E

E

A

A

A

L

V

L

L

F

-

A

-

S

C

K

E

A

A

G

L

A

N

D

L

P

P

A

F

R

D

V

V

R

A

Q

V

A

K

L

V

M

M

G

S

G

G

F

T

A

A

S

A

S

G

K

K

-

G

-

H

V

F

L

T

E

T

V

S

H

W

G

P

E

N

R

K

M

V

I

L

K

S

R

G

T

D

F

L

D

S

P

P

G

A

F

F

R

M

I

I

G

D

L

L

H

A

Q

H

K
|
K

D

D

L

L

N

G

L

I

A

A

L

L

S

D

N

V

A

A

R

N

E

Q

M

L

G

H

V

V

S

P

L

M

P

P

N

L

T

G

A

A

T

A

C

S

Q

R

E

E

L

V

F

Y

N

S

A

Q

C

A

A

R

S

A

H

A

G

G

G

R

S

G

D

R

W

Q

D

D

H

W

S

S

A

A

L

I

V

L

R

E

A

Q

L

V

E

R

L

V

M

S

A

A

D31 (≠ S19) binding
L65 (≠ V53) binding
T96 (≠ S84) binding
G122 (≠ S110) mutation to S: 25-fold decrease in catalytic efficiency with SLA as substrate. 5-fold decrease in catalytic efficiency with NADH as substrate.
R123 (≠ G111) binding ; mutation to G: 130-fold decrease in catalytic efficiency with SLA as substrate. 3-fold decrease in catalytic efficiency with NADH as substrate.
T124 (≠ G112) mutation to G: 230-fold decrease in catalytic efficiency with SLA as substrate. 12-fold decrease in catalytic efficiency with NADH as substrate.
NNYMS 174:178 (≠ NQII- 162:165) binding
K240 (= K227) binding

Sites not aligning to the query:

11:12 binding

Q9I5I6 NAD-dependent L-serine dehydrogenase; L-serine 3-dehydrogenase (NAD(+)); EC 1.1.1.387 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
34% identity, 81% coverage: 1:231/286 of query aligns to 12:250/298 of Q9I5I6

query
sites
Q9I5I6

M
|
M

G
|
G

T
x
A

P
|
P

M
|
M

A
|
A

A
x
T

N
|
N

L
|
L

I
x
L

A
x
K

G
x
A

G
|
G

H
x
Y

E
x
L

V
x
L

S
x
N

L
x
V

S
x
F

S
x
D

-

L

-

V

R

Q

S

S

G

A

V

V

P

-

Q

D

S

G

L

L

L

V

D

A

S

A

G

G

G

A

V

S

A

A

C

A

S

R

S

S

G

A

K

R

E

D

V

A

A

V

E

Q

R

G

A

A

D

D

V

V

I

V

F

I

L

S

M

M

V

L

P
|
P

D

A

T

S

P

Q

H

H

V

V

E

E

A

G

A

L

L

Y

F

L

N

D

G

D

G

G

I

L

A

L

A

A

G

H

L

I

S

A

A

P

G

G

K

T

I

L

V

V

V

L

D

E

M

C

S

S

S
x
T

I

I

S

A

P

P

I

T

A

S

T

A

K

R

A

K

F

I

A

H

E

A

R

A

V

A

N

R

A

E

L

R

G

G

C

L

Q

A

Y

M

L

L

D

D

A

A

P

P

V

V

S
|
S

G

G

E

T

V

A

G

G

A

A

R

A

N

A

A

G

T

T

L

L

S

T

I

F

M

M

V

V

G

G

P

D

Q

A

A

E

T

A

F

L

E

E

T

K

V

A

R

R

P

P

L

L

F

F

E

E

L

A

M

M

G

G

K

R

N

N

I

I

T

F

L

H

V

A

G

G

D

P

N

D

G

G

A

A

G

G

Q

Q

T

V

A

A

K
|
K

V

V

A

C

N

N

Q
x
N

I

Q

I

L

V

L

A

A

L

V

N

L

I

M

E

I

A

G

V

T

A

A

E

E

A

A

L

M

L

A

F

L

A

G

S

V

K

A

A

N

G

G

A

L

D

E

P

A

A

K

R

V

V

L

R

A

Q

E

A

I

L

M

M

R

G

R

G

S

F

S

A

G

S

G

S

N

K
x
W

V

A

L

L

E

E

V

V

H
x
Y

G

N

E

P

R

W

-

P

-

G

-

V

-

M

-

E

-

N

-

A

M

P

I

A

K

S

R

R

T

D

F

Y

D

S

P

G

G

G

F

F

R

M

I

A

G

Q

L

L

H

M

Q

A

K
|
K

D
|
D

L

L

N

G

L

L

P66 (= P54) binding
T96 (≠ S84) binding ; mutation to A: Almost abolished activity.
S122 (= S110) mutation to A: Strongly reduced activity.
K171 (= K159) active site
N175 (≠ Q163) mutation to A: Strongly reduced activity.
W214 (≠ K202) mutation to A: Almost abolished activity.
Y219 (≠ H207) mutation to A: Strongly reduced activity.
K246 (= K227) binding ; mutation to A: Almost abolished activity.
D247 (= D228) mutation to A: Almost abolished activity.

Sites not aligning to the query:

2:31 binding

3q3cA Crystal structure of a serine dehydrogenase from pseudomonas aeruginosa pao1 in complex with NAD (see paper)
34% identity, 81% coverage: 1:231/286 of query aligns to 11:248/294 of 3q3cA

query
sites
3q3cA

M
|
M

G

G

T

A

P

P

M

M

A

A

A

T

N

N

L

L

I

L

A

K

G

A

G

G

H

Y

E

L

V

L

S

N

L

V

S
x
F

S
x
D

-
x
L

-
x
V

R

Q

S

S

G

A

V

V

P

-

Q

D

S

G

L

L

L

V

D

A

S

A

G

G

G

A

V

S

A

A

C

A

S

R

S

S

G

A

K

R

E

D

V

A

A

V

E

Q

R

G

A

A

D

D

V

V

I

V

F

I

L

S

M
|
M

V
x
L

P
|
P

D

A

T

S

P

Q

H

H

V

V

E

E

A

G

A

-

L
|
L

F

Y

N

L

N

D

G

D

G

G

I

L

A

L

A

A

G

H

L

I

S

A

A

P

G

G

K

T

I

L

V

V

V

L

D

E

M

C

S

S

S
x
T

I

I

S

A

P

P

I

T

A

S

T

A

K

R

A

K

F

I

A

H

E

A

R

A

V

A

N

R

A

E

L

R

G

G

C

L

Q

A

Y

M

L

L

D

D

A

A

P

P

V
|
V

S

S

G
|
G

G

G

E

T

V

A

G

G

A

A

R

A

N

A

A

G

T

T

L

L

S

T

I

F

M

M

V

V

G

G

P

D

Q

A

A

E

T

A

F

L

E

E

T

K

V

A

R

R

P

P

L

L

F

F

E

E

L

A

M

M

G

G

K

R

N

N

I

I

T

F

L

H

V

A

G

G

D

P

N

D

G

G

A

A

G

G

Q

Q

T

V

A

A

K
|
K

V

V

A

C

N

N

Q

N

I

Q

I

L

V

L

A

A

L

V

N

L

I

M

E

I

A

G

V

T

A

A

E

E

A

A

L

M

L

A

F

L

A

G

S

V

K

A

A

N

G

G

A

L

D

E

P

A

A

K

R

V

V

L

R

A

Q

E

A

I

L

M

M

R

G

R

G

S

F

S

A

G

S

G

S

N

K

W

V

A

L

L

E

E

V

V

H

Y

G

N

E

P

R

W

-

P

-

G

-

V

-

M

-

E

-

N

-

A

M

P

I

A

K

S

R

R

T

D

F

Y

D

S

P

G

G

G

F
|
F

R

M

I

A

G

Q

L
|
L

H

M

Q

A

K
|
K

D

D

L

L

N

G

L

L

binding nicotinamide-adenine-dinucleotide: M11 (= M1), F29 (≠ S19), D30 (≠ S20), L31 (vs. gap), V32 (vs. gap), M63 (= M52), L64 (≠ V53), P65 (= P54), L73 (= L63), T94 (≠ S84), V119 (= V109), G121 (= G111), K169 (= K159), F237 (= F220), L241 (= L224), K244 (= K227)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 6, 7, 8, 9, 10

2uyyA Structure of the cytokine-like nuclear factor n-pac
26% identity, 95% coverage: 1:272/286 of query aligns to 17:289/292 of 2uyyA

query
sites
2uyyA

M
|
M

G

G

T

S

P

G

M

I

A

V

A

S

N

N

L

L

I

L

A

K

G

M

G

G

H

H

E

T

V

V

S

T

L

V

S
x
W

S
x
N

R
|
R

S
x
T

G

A

V

E

P

K

Q

C

S

D

L

L

-

F

L

I

D

Q

S

E

G

G

G

A

V

R

A

L

C

G

S

R

S

T

G

P

K

A

E

E

V

V

A

V

E

S

R

T

A

C

D

D

V

I

I

T

F

F

L

A

M
x
C

V
|
V

P
x
S

D

D

T

P

P

K

H
x
A

V

A

E

K

A
x
D

A

L

L

V

F

L

N

G

N

P

G

S

G

G

I

V

A

L

A

Q

G

G

L

I

S

R

A

P

G

G

K

K

I

C

V

Y

V

V

D

D

M

M

S
|
S

S
x
T

I

V

S

D

P

A

I

D

A

T

T

V

K

T