SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS23430 FitnessBrowser__Cup4G11:RR42_RS23430 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

O87170 2-pyrone-4,6-dicarboxylate hydrolase; PDC hydrolase; 2-pyrone-4,6-dicarboxylate lactonase; EC 3.1.1.57 from Sphingobium sp. (strain NBRC 103272 / SYK-6) (see paper)
31% identity, 97% coverage: 9:270/271 of query aligns to 27:288/293 of O87170

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O87170

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HCH 29:31 (≠ HTH 11:13) binding
Y47 (= Y29) binding
S75 (≠ A57) binding
R122 (= R104) binding
R128 (≠ H110) binding
Y154 (≠ H137) binding
H178 (= H161) binding
D246 (= D228) mutation to A: Loss of catalytic activity.; mutation to N: Almost inactive.
N251 (= N233) binding

4di8A Crystal structure of the d248a mutant of 2-pyrone-4,6-dicarboxylic acid hydrolase from sphingomonas paucimobilis complexed with substrate at ph 8.5 (see paper)
30% identity, 97% coverage: 9:270/271 of query aligns to 28:289/294 of 4di8A

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4di8A

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active site: A247 (≠ D228)
binding (1E,3Z)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylic acid: H30 (= H11), H32 (= H13), Y48 (= Y29), A75 (= A56), S76 (≠ A57), R123 (= R104), R129 (≠ H110), L130 (= L111), Y155 (≠ H137), H179 (= H161), R216 (= R197), N252 (= N233)
binding 2-oxo-2H-pyran-4,6-dicarboxylic acid: H30 (= H11), Y48 (= Y29), A75 (= A56), S76 (≠ A57), R123 (= R104), R129 (≠ H110), L130 (= L111), Y155 (≠ H137), R216 (= R197), N252 (= N233)

A9CEQ7 D-galactarolactone isomerase; GLI; Galactaro delta-lactone isomerase; EC 5.4.1.4 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see paper)
21% identity, 97% coverage: 9:271/271 of query aligns to 23:285/292 of A9CEQ7

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N240 (≠ D228) mutation to D: 28-fold decrease in enzymatic activity. Does not gain the ability to hydrolyze D-galactaro-1,5-lactone or D-galactaro-1,4-lactone.

Query Sequence

>RR42_RS23430 FitnessBrowser__Cup4G11:RR42_RS23430
MSVSTPVWDCHTHIYGPYDRYPLPKEAVYVPEAAPFDALRAVHKSIGITHGVIVQAANYG
PNHCALLAALDAGNGAYRGIAVISPEIPDEKLAMMAARGVKGIRIGLMSHLGNSFDAGKV
RALVERIRPYGWHALIHGEPENVMAAADAAGHYGVKLVIDHMARLSLDAPDLTARLDDIC
TLLRHEHVWIKVSGVDRITKGRLDAPEAKAVLQRLVETAPKRAIWGTDWPHPNLTYPVPK
DDALLSWLGSAVGDEAMLKAVLSENPASLYQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory