SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS24555 FitnessBrowser__Cup4G11:RR42_RS24555 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
61% identity, 97% coverage: 14:491/494 of query aligns to 2:479/482 of P25526

query
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P25526

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WN 156:157 (= WN 168:169) binding
KPAS 180:183 (≠ RPAD 192:195) binding
GS 233:234 (= GS 245:246) binding
L256 (= L268) binding
E386 (= E398) binding

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
60% identity, 97% coverage: 14:491/494 of query aligns to 1:478/481 of 3jz4A

query
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3jz4A

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active site: N156 (= N169), K179 (≠ R192), E254 (= E267), C288 (= C301), E385 (= E398), E462 (= E475)
binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P167), W155 (= W168), K179 (≠ R192), A181 (= A194), S182 (≠ D195), A212 (≠ S225), G216 (= G229), G232 (= G245), S233 (= S246), I236 (≠ V249), C288 (= C301), K338 (≠ H351), E385 (= E398), F387 (= F400)

8c54A Cryo-em structure of nadh bound sla dehydrogenase rlgabd from rhizobium leguminosarum bv. Trifolii srd1565
60% identity, 96% coverage: 15:490/494 of query aligns to 2:477/482 of 8c54A

query
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8c54A

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D

K

E

S

K

A

A

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A

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K

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M

Q

E

A

E

M

H

I

I

D

A

D

D

A

A

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M

G

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N

G

G

A

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T

G

G

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K

G

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S

A

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L

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G

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F

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E

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T

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I

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G

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T

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Q

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T

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A

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A

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F

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D

H

E

E

A

N

E

D

A

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I

I

A

A

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M

A

A

N

N

D

D

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E

E

Y

F

G

G

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L

A

A

A

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Y

Y

L

F

Y

Y

T

A

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D

D

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M

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A

E

E

A

A

L

L

E

E

Y

A

G

G

M

M

V

V

G

G

L

V

N

N

T

T

G

G

L

M

I

I

S

A

N

N

E

E

V

M

A

A

P

P

F

F

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G

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I

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Q

Q

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S

G

G

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T

G

G

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R

E

E

G

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Y

Y

G

G

I

I

D

E

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L

E

E

I

L

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K

Y

Y

L

V

binding 1,4-dihydronicotinamide adenine dinucleotide: I152 (= I165), T153 (= T166), P154 (= P167), K179 (≠ R192), A212 (≠ S225), K213 (≠ R226), F230 (= F243), T231 (= T244), G232 (= G245), S233 (= S246), V236 (= V249), W239 (≠ V252), G256 (= G269)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
55% identity, 96% coverage: 19:491/494 of query aligns to 57:531/535 of P51649

query
sites
P51649

S

A

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T

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A

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A

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A

A

S

A

G

A

G

-

R

-

S

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F

F

A

P

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Q

N

D

P

P

A

A

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G

G

E

A

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x
C

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x
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x
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N

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|
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F

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K

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C

C93 (≠ L57) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G140) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ P144) to Y: 83% of activity; dbSNP:rs2760118
P182 (= P146) to L: 48% of activity; dbSNP:rs3765310
R213 (= R177) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C187) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (≠ RPAD 192:195) binding
T233 (= T197) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A201) to S: 65% of activity; dbSNP:rs62621664
N255 (≠ Q219) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G229) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTEVG 245:250) binding
R334 (= R295) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (= N296) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C301) modified: Disulfide link with 342, In inhibited form
C342 (= C303) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (= N332) natural variant: N -> S
P382 (= P342) to L: in SSADHD; 2% of activity
V406 (= V366) to I: in dbSNP:rs143741652
G409 (= G369) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (= S458) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832
533 G → R: in SSADHD; <1% of activity; dbSNP:rs72552284

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
55% identity, 96% coverage: 19:491/494 of query aligns to 7:481/485 of 2w8rA

query
sites
2w8rA

S

A

L

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A

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C

A

A

A

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I

I

T
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T

P

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W

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|
N

F

F

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x
K

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A

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A

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x
A

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T

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E

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A

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x
A

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A

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N

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E

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C

active site: N155 (= N169), K178 (≠ R192), E256 (= E267), A290 (≠ C301), E388 (= E398), E465 (= E475)
binding adenosine-5'-diphosphate: T152 (= T166), K178 (≠ R192), A214 (≠ S225), S235 (= S246), T238 (≠ V249)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
55% identity, 96% coverage: 19:491/494 of query aligns to 7:481/485 of 2w8qA

query
sites
2w8qA

S

A

L

L

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T

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A

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A

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W

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N

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A

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I

L

L

Y

Y

A

S

A

A

S

F

F

F

V

L

E

E

W

W

F

F

A

S

E

E

A

A

K

R

R

R

I

V

Y

Y

G

G

D

D

V

I

A

I

P

H

H

T

P

P

Q

A

T

K

D

D

K

R

R

R

I

A

L

L

V

V

I

L

R

K

Q

Q

P

P

V

I

G

G

V

V

C

A

A

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R
|
R

K

K

V

V

A

G

P

A

A

A

L

L

A

A

G

G

C

C

S

T

I

V

V

V

R
x
K

P

P

A

A

D

E

L

D

T

T

P

P

L

F

T

S

A

A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

S

R

Q

A

A

G

G

I

I

P

P

A

S

G

G

V

V

L

Y

Q

N

M

V

V

I

-

P

C

C

G

S

-

R

-

K

P

N

S

A

R

K

E

E

I

V

G

G

A

E

V

A

L

I

T

C

A

T

S

D

P

P

V

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G

G

S

S

T

T

E

T

V

T

G

G

R

K

V

I

L

L

M

L

S

H

Q

H

S

A

P

N

T

S

I

V

K

K

R

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

A

Q

A

A

I

V

E

A

G

G

A

A

M

M

A

A

S

S

K

K

Y

F

R
|
R

N

N

S

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

F

F

L

L

V

V

Q

Q

D

R

G

G

I

I

Y

H

D

D

R

A

F

F

V

V

E

K

A

A

L

F

V

A

R

E

R

A

V

M

A

K

E

K

-

N

L

L

K

R

V

V

G

G

N

N

G

G

V

F

E

E

P

E

G

G

V

T

Q

T

Q

Q

G

G

P

P

L

L

I

I

Q

N

K

E

S

K

A

A

C

V

E

E

H

K

L

V

Q

E

A

K

M

Q

I

V

D

N

D

D

A

A

V

V

G

S

K

K

G

G

A

A

K

T

V

V

V

T

G

G

K

K

G

R

H

H

A

Q

L

L

G

G

G

K

T

N

F

F

E

E

P

P

T

T

V

L

I

L

A

C

G

N

A

V

T

T

P

Q

D

D

M

M

R

L

V

C

A

T

R

H

E

E

E
|
E

L

T

F

F

G

G

P

P

V

L

A

A

P

P

V

V

F

I

R

K

F

F

R

D

D

T

E

E

A

E

E

E

A

A

I

I

A

A

L

I

A

A

N

N

D

A

T

A

E

D

Y

V

G

G

L

L

A

A

A

G

Y

Y

L

F

Y

Y

T

S

R

Q

D

D

N

P

A

A

R

Q

I

I

W

W

R

R

V

V

G

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

L

V

N

N

T

E

G

G

L

L

I

I

S
|
S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

R

K

Y

Y

G

G

I

I

D

D

E

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

L

V

C

C

active site: N155 (= N169), K178 (≠ R192), E256 (= E267), A290 (≠ C301), E388 (= E398), E465 (= E475)
binding succinic acid: R163 (= R177), R284 (= R295), A290 (≠ C301), S448 (= S458)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
41% identity, 96% coverage: 19:491/494 of query aligns to 1:473/476 of 5x5uA

query
sites
5x5uA

S

T

L

Y

L

T

E

D

T

T

R

Q

A

L

W

L

L

I

A

D

S

G

G

E

W

W

Q

V

E
x
D

A
|
A

S
x
A

G

S

G
|
G

R

K

S

T

F

I

A

D

V

V

T

V

N

N

P

P

A

A

T

T

G

G

E

K

I

P

L

I

A

G

R

R

V

V

A

A

S

H

L

A

S

G

G

I

A

A

E

D

V

L

E

D

S

R

A

A

I

L

T

A

A

A

S

A

A

Q

Q

S

A

G

Q

F

A

E

V

A

W

W

Q

R

R

K

R

V

S

P

S

A

H

H

E

E

R

R

A

A

K

A

L

T

L

M

R

R

A

K

W

A

F

A

D

A

L

L

M

V

I

R

A

E

N

R

A

A

D

D

D

A

L

I

A

A

L

Q

I

L

M

M

T

T

S

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

R

R

G

V

E

E

I

V

L

L

Y

S

A

A

S

D

F

I

V

I

E

E

W

W

F

F

A

A

E

D

E

E

A

G

K

R

R

R

I

V

Y

Y

G

G

D

R

V

I

A

V

P

P

H

P

P

R

Q

N

T

L

D

G

K

A

R

Q

I

Q

L

T

V

V

I

V

R

K

Q

E

P

P

V

V

G

G

V

P

C

V

A

A

I

F

T

T

P
|
P

W

W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

V

L

A

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

S

S

I

F

I

L

V

V

R
x
K

P

A

A

P

D

E

L

E

T

T

P

P

L

A

T

S

A

P

L

A

A

A

L

L

A

L

V

R

L

A

A

F

E

V

R

D

A

A

G

G

I

V

P

P

A

A

G

G

V

V

L

I

Q

G

M

L

V

V

C

Y

G

G

P

D

S
x
P

R
x
A

E

E

I

I

G
x
S

A

S

V

Y

L

L

T

I

A

P

S

H

P

P

V

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G
|
G

S
|
S

T

T

E

P

V
|
V

G

G

R

K

V

Q

L

L

M

A

S

S

Q

L

S

A

S

G

P

L

T

H

I

M

K

K

R

R

L

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

D

D

L

V

D

A

A

L

A

A

I

V

E

K

G

A

A

A

M

G

A

G

S

A

K

K

Y

F

R

R

N

N

S

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

A

P

N

T

R

R

F

F

L

L

V

V

Q

H

D

N

G

S

I

I

Y

R

D

D

R

E

F

F

V

T

E

R

A

A

L

L

V

V

R

K

R

H

V

A

A

E

E

G

L

L

K

K

V

V

G

G

N

N

G

G

V

L

E

E

P

E

G

G

V

T

Q

T

Q

L

G

G

P

A

L

L

I

A

Q

N

K

P

S
x
R

A
x
R

C

L

E

T

H

A

L

M

Q

A

A

S

M

V

I

I

D

D

D

N

A

A

V

R

G

K

K

V

G

G

A

A

K

S

V

I

V

E

V

T

G

G

K

E

G

R

H

I

A

G

L

S

G

E

G

G

T

N

F

F

E

A

P

P

T

T

V

V

I

I

A

A

G

N

A

V

T

P

P

L

D

D

M

A

R

D

V

V

A

F

R

N

E

N

E
|
E

L

P

F
|
F

G

G

P

P

V

V

A

A

P

A

V

I

F

R

R

G

F

F

R

D

D

K

E

L

A

E

E

E

A

A

I

I

A

A

L

E

A

A

N

N

D

R

T

L

E

P

Y

F

G

G

L

L

A

A

A

G

Y

Y

L

A

Y

F

T

T

R

R

D

S

N

F

A

A

R

N

I

V

W

H

R

L

V

L

G

T

E

Q

A

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

L

I

N

N

T

Q

G

P

L

A

I

T

S

P

N

W

E

P

V

E

A

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L

Y

G

G

R

S

E
|
E

G

G

S

G

R

P

Y

E

G

A

I

L

D

E

E

P

Y

Y

L

L

E

V

I

T

K

K

Y

S

L

V

C

T

active site: N151 (= N169), K174 (≠ R192), E249 (= E267), C283 (= C301), E380 (= E398), E457 (= E475)
binding glycerol: D15 (≠ E33), A16 (= A34), A17 (≠ S35), G19 (= G37)
binding nicotinamide-adenine-dinucleotide: P149 (= P167), P207 (≠ S225), A208 (≠ R226), S211 (≠ G229), G227 (= G245), S228 (= S246), V231 (= V249), R329 (≠ S347), R330 (≠ A348), E380 (= E398), F382 (= F400)

5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
41% identity, 96% coverage: 19:491/494 of query aligns to 1:473/476 of 5x5tA

query
sites
5x5tA

S

T

L

Y

L

T

E

D

T

T

R

Q

A

L

W

L

L

I

A

D

S

G

G

E

W

W

Q

V

E

D

A

A

S

A

G

S

G

G

R

K

S

T

F

I

A

D

V

V

T

V

N

N

P

P

A

A

T

T

G

G

E

K

I

P

L

I

A

G

R

R

V

V

A

A

S

H

L

A

S

G

G

I

A

A

E

D

V

L

E

D

S

R

A

A

I

L

T

A

A

A

S

A

A

Q

Q

S

A

G

Q

F

A

E

V

A

W

W

Q

R

R

K

R

V

S

P

S

A

H

H

E

E

R

R

A

A

K

A

L

T

L

M

R

R

A

K

W

A

F

A

D

A

L

L

M

V

I

R

A

E

N

R

A

A

D

D

D

A

L

I

A

A

L

Q

I

L

M

M

T

T

S

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

R

R

G

V

E

E

I

V

L

L

Y

S

A

A

S

D

F

I

V

I

E

E

W

W

F

F

A

A

E

D

E

E

A

G

K

R

R

R

I

V

Y

Y

G

G

D

R

V

I

A

V

P

P

H

P

P

R

Q

N

T

L

D

G

K

A

R

Q

I

Q

L

T

V

V

I

V

R

K

Q

E

P

P

V

V

G

G

V

P

C

V

A

A

I

F

T

T

P

P

W

W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

V

L

A

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

S

S

I

F

I

L

V

V

R
x
K

P

A

A

P

D

E

L

E

T

T

P

P

L

A

T

S

A

P

L

A

A

A

L

L

A

L

V

R

L

A

A

F

E

V

R

D

A

A

G

G

I

V

P

P

A

A

G

G

V

V

L

I

Q

G

M

L

V

V

C

Y

G

G

P

D

S

P

R

A

E

E

I

I

G

S

A

S

V

Y

L

L

T

I

A

P

S

H

P

P

V

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G

G

S

S

T

T

E

P

V

V

G

G

R

K

V

Q

L

L

M
x
A

S

S

Q

L

S

A

S

G

P

L

T

H

I

M

K

K

R

R

L

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

D

D

L

V

D

A

A

L

A

A

I

V

E

K

G

A

A

A

M

G

A

G

S

A

K

K

Y

F

R

R

N

N

S

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

A

P

N

T

R

R

F

F

L

L

V

V

Q

H

D

N

G

S

I

I

Y

R

D

D

R

E

F

F

V

T

E

R

A

A

L

L

V

V

R

K

R

H

V

A

A

E

E

G

L

L

K

K

V

V

G

G

N

N

G

G

V

L

E

E

P

E

G

G

V

T

Q

T

Q

L

G

G

P

A

L

L

I

A

Q

N

K

P

S

R

A

R

C

L

E

T

H

A

L

M

Q

A

A

S

M

V

I

I

D

D

D

N

A

A

V

R

G

K

K

V

G

G

A

A

K

S

V

I

V

E

V

T

G

G

K

E

G

R

H

I

A

G

L

S

G

E

G

G

T

N

F

F

E

A

P

P

T

T

V

V

I

I

A

A

G

N

A

V

T

P

P

L

D

D

M

A

R

D

V

V

A

F

R

N

E

N

E
|
E

L

P

F

F

G

G

P

P

V

V

A

A

P

A

V

I

F

R

R

G

F

F

R

D

D

K

E

L

A

E

E

E

A

A

I

I

A

A

L

E

A

A

N

N

D

R

T

L

E

P

Y

F

G

G

L

L

A

A

A

G

Y

Y

L

A

Y

F

T

T

R

R

D

S

N

F

A

A

R

N

I

V

W

H

R

L

V

L

G

T

E

Q

A

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

L

I

N

N

T

Q

G

P

L

A

I

T

S

P

N

W

E

P

V

E

A

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L
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active site: N151 (= N169), K174 (≠ R192), E249 (= E267), C283 (= C301), E380 (= E398), E457 (= E475)
binding glycerol: A236 (≠ M254), Y454 (≠ L472)

6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
37% identity, 95% coverage: 22:488/494 of query aligns to 1:471/477 of 6j76A

query
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6j76A

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active site: N148 (= N169), E246 (= E267), C280 (= C301), E458 (= E475)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I144 (= I165), T145 (= T166), A146 (≠ P167), W147 (= W168), N148 (= N169), K171 (≠ R192), T173 (≠ A194), S174 (≠ D195), G204 (≠ S225), G208 (= G229), T223 (= T244), G224 (= G245), S225 (= S246), A228 (≠ V249), S231 (≠ V252), I232 (≠ L253), E246 (= E267), L247 (= L268), C280 (= C301), E381 (= E398), F383 (= F400), H447 (≠ F464)

Q63639 Retinal dehydrogenase 2; RALDH 2; RalDH2; Aldehyde dehydrogenase family 1 member A2; ALDH1A2; Retinaldehyde-specific dehydrogenase type 2; RALDH(II); EC 1.2.1.36 from Rattus norvegicus (Rat) (see paper)
37% identity, 96% coverage: 23:494/494 of query aligns to 38:513/518 of Q63639

query
sites
Q63639

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