SitesBLAST
Comparing RR42_RS24700 FitnessBrowser__Cup4G11:RR42_RS24700 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
38% identity, 94% coverage: 2:528/560 of query aligns to 2:451/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G11), G13 (= G13), S14 (= S14), A15 (≠ G15), E35 (= E35), A36 (= A36), W47 (= W62), P65 (= P80), G67 (= G82), V180 (= V220), A214 (= A254), G215 (= G255), A218 (≠ Q258), T270 (= T337), Y391 (= Y468), A424 (= A501), I435 (≠ T512), N436 (= N513)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
38% identity, 95% coverage: 3:533/560 of query aligns to 22:575/578 of 5nccA
- active site: R347 (≠ L321), L420 (≠ V383), I421 (≠ C384), S507 (≠ V467), A509 (≠ H469), G552 (= G510), Q553 (≠ N511)
- binding flavin-adenine dinucleotide: G30 (= G11), G32 (= G13), T33 (≠ S14), A34 (≠ G15), L53 (= L34), E54 (= E35), A55 (= A36), F74 (≠ M55), W80 (= W62), A98 (≠ P80), G100 (= G82), G105 (= G87), S106 (= S88), N110 (= N92), A111 (= A93), T112 (≠ M94), L113 (≠ V95), V238 (= V220), A278 (= A254), H282 (≠ Q258), L286 (= L262), N508 (≠ Y468), Q553 (≠ N511), T554 (= T512), G555 (≠ N513), V558 (≠ T516)
6yrvAAA structure of fap after illumination at 100k (see paper)
37% identity, 96% coverage: 3:539/560 of query aligns to 6:572/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H360), N499 (≠ Y468)
- binding flavin-adenine dinucleotide: G14 (= G11), G16 (= G13), T17 (≠ S14), A18 (≠ G15), L37 (= L34), E38 (= E35), A39 (= A36), F58 (≠ M55), W64 (= W62), A82 (≠ P80), G89 (= G87), S90 (= S88), N94 (= N92), A95 (= A93), T96 (≠ M94), L97 (≠ V95), M191 (≠ T188), V222 (= V220), C264 (≠ A253), A265 (= A254), G266 (= G255), H269 (≠ Q258), N499 (≠ Y468), A534 (= A501), Q544 (≠ N511), T545 (= T512), G546 (≠ N513)
- binding heptadecane: V377 (= V362), G379 (≠ A364), M380 (= M365), G386 (vs. gap), T389 (≠ N368), Y390 (≠ H369), F393 (vs. gap), T408 (≠ S380), Q410 (≠ H382)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
38% identity, 95% coverage: 3:533/560 of query aligns to 82:642/654 of A0A248QE08
- TA 93:94 (≠ SG 14:15) binding
- E114 (= E35) binding
- L162 (≠ M84) binding
- S166 (= S88) binding
- NATL 170:173 (≠ NAMV 92:95) binding
- V298 (= V220) binding
- C432 (≠ A340) binding
- R451 (≠ H360) binding
- Y466 (≠ H369) binding
- Q486 (≠ H382) binding
- G622 (≠ N513) binding
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
36% identity, 94% coverage: 2:528/560 of query aligns to 11:527/532 of 4mjwA
- active site: I333 (≠ G342), P377 (≠ V383), N378 (≠ C384), V464 (= V467), H466 (= H469), V509 (≠ G510), N510 (= N511)
- binding flavin-adenine dinucleotide: G20 (= G11), G22 (= G13), S23 (= S14), E44 (= E35), A45 (= A36), W71 (= W62), R89 (= R81), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ A93), I103 (≠ V95), R231 (≠ Q219), A232 (≠ V220), T269 (≠ A254), G270 (= G255), D273 (≠ Q258), Y465 (= Y468), H466 (= H469), A500 (= A501), N510 (= N511), P511 (≠ T512), N512 (= N513), V515 (≠ T516)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
36% identity, 94% coverage: 2:528/560 of query aligns to 11:527/527 of 2jbvA
- active site: I333 (≠ G342), P377 (≠ V383), N378 (≠ C384), V464 (= V467), H466 (= H469), V509 (≠ G510), N510 (= N511)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G13), S23 (= S14), E44 (= E35), A45 (= A36), W71 (= W62), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ A93), I103 (≠ V95), R231 (≠ Q219), A232 (≠ V220), T269 (≠ A254), G270 (= G255), D273 (≠ Q258), V464 (= V467), Y465 (= Y468), H466 (= H469), D499 (= D500), A500 (= A501), N510 (= N511), P511 (≠ T512), N512 (= N513), V515 (≠ T516)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
36% identity, 94% coverage: 2:528/560 of query aligns to 11:527/530 of 3ljpA
- active site: I333 (≠ G342), P377 (≠ V383), N378 (≠ C384), A464 (≠ V467), H466 (= H469), V509 (≠ G510), N510 (= N511)
- binding dihydroflavine-adenine dinucleotide: G22 (= G13), S23 (= S14), E44 (= E35), A45 (= A36), W71 (= W62), R89 (= R81), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ A93), I103 (≠ V95), A232 (≠ V220), T269 (≠ A254), D273 (≠ Q258), Y465 (= Y468), H466 (= H469), D499 (= D500), A500 (= A501), N510 (= N511), P511 (≠ T512), N512 (= N513), V515 (≠ T516)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
32% identity, 94% coverage: 5:529/560 of query aligns to 2:563/565 of 5oc1A
- active site: V339 (≠ S322), N413 (≠ V383), A414 (≠ C384), I499 (≠ V467), H501 (= H469), A544 (≠ G510), H545 (≠ N511)
- binding 4-methoxybenzoic acid: Y91 (≠ A93), I356 (≠ F339), I390 (≠ A364), F396 (≠ A370), T412 (≠ H382), I499 (≠ V467), H501 (= H469), H545 (≠ N511)
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), N11 (≠ S14), A12 (≠ G15), E32 (= E35), A33 (= A36), W60 (= W62), P78 (= P80), G80 (= G82), G85 (= G87), S86 (= S88), H90 (≠ N92), Y91 (≠ A93), V93 (= V95), V230 (= V220), S270 (≠ A253), A271 (= A254), G272 (= G255), F500 (≠ Y468), H545 (≠ N511), T546 (= T512), Q547 (≠ N513), I550 (≠ T516)
8bxlB Patulin synthase from penicillium expansum
35% identity, 94% coverage: 2:529/560 of query aligns to 11:588/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G11), G22 (= G13), T23 (≠ S14), A24 (≠ G15), E44 (= E35), A45 (= A36), W80 (vs. gap), G100 (= G82), G105 (= G87), S106 (= S88), R109 (≠ I91), N110 (= N92), Y111 (≠ A93), A113 (≠ V95), L253 (≠ Q219), A254 (≠ V220), A288 (= A254), Q292 (= Q258), F525 (≠ Y468), D559 (= D500), A560 (= A501), H570 (≠ N511), P571 (≠ T512), Q572 (≠ N513), L575 (≠ T516)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
32% identity, 94% coverage: 5:529/560 of query aligns to 2:563/565 of 3fimB
- active site: V339 (≠ S322), N413 (≠ V383), A414 (≠ C384), I499 (≠ V467), H501 (= H469), A544 (≠ G510), H545 (≠ N511)
- binding flavin-adenine dinucleotide: G8 (= G11), N11 (≠ S14), A12 (≠ G15), E32 (= E35), A33 (= A36), W60 (= W62), P78 (= P80), G80 (= G82), G85 (= G87), S86 (= S88), H90 (≠ N92), Y91 (≠ A93), V93 (= V95), V230 (= V220), S270 (≠ A253), A271 (= A254), F500 (≠ Y468), H501 (= H469), H545 (≠ N511), T546 (= T512), Q547 (≠ N513), I550 (≠ T516)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
33% identity, 93% coverage: 5:527/560 of query aligns to 6:527/531 of E4QP00
- V101 (≠ I91) mutation to H: Abolishes activity.
- M103 (≠ A93) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ H382) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ C384) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (= V467) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y468) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H469) mutation to A: Abolishes activity.
- N511 (= N511) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
33% identity, 93% coverage: 5:527/560 of query aligns to 2:523/525 of 4udqA
- active site: L331 (≠ R332), F364 (≠ V383), W365 (≠ C384), V461 (= V467), H463 (= H469), A506 (≠ G510), N507 (= N511)
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), T11 (≠ S14), A12 (≠ G15), E32 (= E35), A33 (= A36), W64 (= W62), G88 (= G82), G93 (= G87), G94 (≠ S88), N98 (= N92), M99 (≠ A93), V101 (= V95), V229 (= V220), T261 (≠ A253), A262 (= A254), W462 (≠ Y468), H463 (= H469), A497 (= A501), N507 (= N511), T508 (= T512), N509 (= N513), T512 (= T516)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
33% identity, 92% coverage: 6:521/560 of query aligns to 3:498/508 of 4ha6A
- active site: F360 (≠ V383), G361 (≠ C384), H444 (≠ V467), H446 (= H469), G487 (= G510), P488 (≠ N511)
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), S11 (= S14), A12 (≠ G15), E32 (= E35), A33 (= A36), W58 (= W62), R77 (= R81), G78 (= G82), G83 (= G87), S84 (= S88), L87 (≠ I91), H88 (≠ N92), A89 (= A93), M90 (= M94), G91 (≠ V95), V218 (= V220), A251 (= A254), G252 (= G255), E255 (≠ Q258), H445 (≠ Y468), A478 (= A501), P488 (≠ N511), I489 (≠ T512), H490 (≠ N513)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (= A93), S314 (≠ R328), H444 (≠ V467), H446 (= H469)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
33% identity, 92% coverage: 6:521/560 of query aligns to 3:498/509 of 3t37A
- active site: F360 (≠ V383), G361 (≠ C384), H444 (≠ V467), H446 (= H469), G487 (= G510), P488 (≠ N511)
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), S11 (= S14), A12 (≠ G15), E32 (= E35), A33 (= A36), W58 (= W62), R77 (= R81), G78 (= G82), R79 (≠ K83), G83 (= G87), S84 (= S88), H88 (≠ N92), A89 (= A93), G91 (≠ V95), R217 (≠ Q219), V218 (= V220), A251 (= A254), E255 (≠ Q258), H445 (≠ Y468), A478 (= A501), P488 (≠ N511), I489 (≠ T512), H490 (≠ N513)
3q9tA Crystal structure analysis of formate oxidase (see paper)
30% identity, 94% coverage: 3:528/560 of query aligns to 5:570/577 of 3q9tA
- active site: A335 (≠ S322), D422 (≠ C384), A508 (≠ V467), H510 (= H469), C552 (≠ G510), R553 (≠ N511)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G13), T16 (≠ S14), E37 (= E35), A38 (= A36), W65 (= W62), T85 (≠ P80), R86 (= R81), G87 (= G82), G92 (= G87), S93 (= S88), N97 (= N92), Y98 (≠ A93), F99 (≠ M94), T100 (≠ V95), S229 (≠ A218), S265 (≠ A253), Q266 (≠ A254), E270 (≠ Q258), F509 (≠ Y468), D542 (= D500), A543 (= A501), R553 (≠ N511), I554 (≠ T512), Q555 (≠ N513), V558 (≠ T516)
5zu2A Effect of mutation (r554a) on fad modification in aspergillus oryzae rib40formate oxidase (see paper)
30% identity, 94% coverage: 3:528/560 of query aligns to 5:570/577 of 5zu2A
- binding flavin-adenine dinucleotide: G15 (= G13), T16 (≠ S14), E37 (= E35), A38 (= A36), W65 (= W62), T85 (≠ P80), R86 (= R81), G87 (= G82), G92 (= G87), S93 (= S88), N97 (= N92), Y98 (≠ A93), T100 (≠ V95), S229 (≠ A218), S265 (≠ A253), Q266 (≠ A254), E270 (≠ Q258), F509 (≠ Y468), D542 (= D500), A543 (= A501), A553 (≠ N511), I554 (≠ T512), Q555 (≠ N513), V558 (≠ T516)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
31% identity, 93% coverage: 5:527/560 of query aligns to 16:572/577 of 4h7uA
- active site: A343 (vs. gap), V426 (≠ C384), Y510 (≠ V467), H512 (= H469), A555 (≠ G510), H556 (≠ N511)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G11), G24 (= G13), T25 (≠ S14), A26 (≠ G15), E46 (= E35), A47 (= A36), W74 (= W62), G99 (= G87), C100 (≠ S88), H103 (≠ I91), N104 (= N92), G105 (≠ A93), V107 (= V95), L242 (≠ Q219), V243 (= V220), G282 (≠ A254), G283 (= G255), A286 (≠ Q258), H512 (= H469), A546 (= A501), H556 (≠ N511), T557 (= T512), Q558 (≠ N513), V561 (≠ T516)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
31% identity, 93% coverage: 5:527/560 of query aligns to 41:597/602 of Q3L245
- N100 (≠ A63) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I91) modified: Tele-8alpha-FAD histidine
- N344 (= N291) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H469) active site, Proton acceptor
- H581 (≠ N511) active site
Sites not aligning to the query:
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
31% identity, 94% coverage: 5:528/560 of query aligns to 4:563/566 of 7vzsA
- binding D-glucal: Y6 (= Y7), L22 (= L23), N25 (≠ D26), Y51 (≠ V52), I349 (≠ E323), Q356 (≠ E330), E411 (≠ H382), E444 (≠ P415), W445 (≠ L416), K448 (≠ E419), R499 (≠ D465), N501 (≠ V467), H546 (≠ N511), K563 (≠ R528)
- binding flavin-adenine dinucleotide: G10 (= G11), G12 (= G13), T13 (≠ S14), S14 (≠ G15), E34 (= E35), A35 (= A36), Y51 (≠ V52), F55 (≠ P56), W61 (= W62), R79 (≠ P80), G81 (= G82), G86 (= G87), T87 (≠ S88), N91 (= N92), G92 (≠ A93), M93 (= M94), A94 (≠ V95), T232 (≠ Q219), A233 (≠ V220), A273 (= A254), G274 (= G255), R277 (≠ Q258), F502 (≠ Y468), A536 (= A501), H546 (≠ N511), L547 (≠ T512), V548 (≠ N513), L551 (≠ T516)
Sites not aligning to the query:
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
31% identity, 94% coverage: 5:528/560 of query aligns to 4:563/569 of 4ynuA
- active site: V341 (≠ L321), F412 (≠ V383), W413 (≠ C384), N501 (≠ V467), H503 (= H469), G545 (= G510), H546 (≠ N511)
- binding flavin-adenine dinucleotide: G12 (= G13), T13 (≠ S14), S14 (≠ G15), E34 (= E35), A35 (= A36), Y51 (≠ V52), F55 (≠ P56), W61 (= W62), R79 (≠ P80), G81 (= G82), G86 (= G87), T87 (≠ S88), N91 (= N92), G92 (≠ A93), T232 (≠ Q219), A233 (≠ V220), A273 (= A254), G274 (= G255), R277 (≠ Q258), F502 (≠ Y468), A536 (= A501), H546 (≠ N511), L547 (≠ T512), V548 (≠ N513), L551 (≠ T516)
- binding D-glucono-1,5-lactone: Y51 (≠ V52), E411 (≠ H382), A496 (≠ Q462), N497 (≠ R463), R499 (≠ D465), R499 (≠ D465), N501 (≠ V467), H503 (= H469), H546 (≠ N511)
Query Sequence
>RR42_RS24700 FitnessBrowser__Cup4G11:RR42_RS24700
MNQQFDYIVVGGGSGGCVVAGRLSEDPNLSVCVLEAGGHGDGMVVKVPAGAVAMMPTRLN
NWAFDTVPQAGLGGRIGYQPRGKMLGGSSAINAMVYIRGHRSDYDRWASLGNSGWSYDDV
LPYFRLSEHNERFDNAWHGRNGPLNVSDLRTDNPFQARYLEAARQAGLPLTDDFNGAQQE
GIGIYQVTQKQGERWSAARAYLHPHIGRRANLTVETHAQVRRILLEGKRAVGVEVLQHGV
IRTLRARREVVLAAGALQTPQLLMLSGVGPAQELARVGIQTVQHLPGVGRNLQDHPDFVF
GYRTGSLDTMGVSLLGSVRILSEILRFRRERRGMLTTNFAEGGGFLKTRPELEAPDIQLH
FVVAMVDNHARRMRLGHGFSCHVCLLRPRSTGSVTLHSNDPLTAPLIDPAFFDDPLDVED
MVAGFKITRRLMQTPALAKWATRDLFTSHVKTDDDIRAVLRQRTDTVYHPVGTCRMGQDE
MAVVDPQLRVHGLDGLRVVDASIMPTLIGGNTNAPTIMIGEKAVDLIRGVSRVTSDVRAQ
AEAAAAQSASHHQKTSHVTT
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory