SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS26155 FitnessBrowser__Cup4G11:RR42_RS26155 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
65% identity, 99% coverage: 5:484/487 of query aligns to 1:480/482 of P25526

query
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P25526

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WN 156:157 (= WN 160:161) binding NADP(+)
KPAS 180:183 (≠ KPAE 184:187) binding NADP(+)
GS 233:234 (= GS 237:238) binding NADP(+)
L256 (= L260) binding NADP(+)
E386 (= E390) binding NADP(+)

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
65% identity, 98% coverage: 7:484/487 of query aligns to 2:479/481 of 3jz4A

query
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3jz4A

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active site: N156 (= N161), K179 (= K184), E254 (= E259), C288 (= C293), E385 (= E390), E462 (= E467)
binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P159), W155 (= W160), K179 (= K184), A181 (= A186), S182 (≠ E187), A212 (≠ P217), G216 (= G221), G232 (= G237), S233 (= S238), I236 (= I241), C288 (= C293), K338 (= K343), E385 (= E390), F387 (= F392)

8c54A Cryo-em structure of nadh bound sla dehydrogenase rlgabd from rhizobium leguminosarum bv. Trifolii srd1565 (see paper)
62% identity, 98% coverage: 7:484/487 of query aligns to 2:479/482 of 8c54A

query
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8c54A

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S

Q

Q

G

G

P

P

L

L

I

I

D

D

A

E

D

K

A

A

V

V

A

A

K

K

V

M

E

E

S

E

H

H

I

I

A

A

D

D

A

A

L

M

A

A

H

N

G

G

A

G

R

T

V

V

L

L

T

T

G

G

K

K

R

R

H

S

A

V

L

L

G

G

T

T

F

F

E

E

P

P

T

T

V

V

A

T

G

G

A

V

T

T

A

Q

A

T

M

M

R

K

V

V

A

A

R

K

E

E

T

T

F

F

G

A

P

P

L

L

A

A

P

P

L

I

F

I

R

S

F

F

K

K

G

H

D

E

Q

N

E

D

V

V

I

I

A

A

M

M

A

A

N

N

D

D

T

S

E

E

S

F

G

G

L

L

A

A

A

S

Y

Y

F

F

F

Y

S

A

K

K

D

D

M

M

A

A

R

R

V

I

W

W

R

R

V

V

A

A

E

E

A

A

L

L

E

E

Y

A

G

G

M

M

V

V

G

G

I

V

N

N

T

T

G

G

L

M

I

I

S

A

N

N

E

E

V

M

A

A

P

P

F

F

G

G

V

I

K

K

Q

Q

S

S

G

G

L

T

G

G

R

R

E

E

G

G

S

S

S

K

Y

Y

G

G

I

I

D

E

E

G

Y

F

L

L

E

E

M

L

K

K

Y

Y

L

V

C

A

L

L

binding 1,4-dihydronicotinamide adenine dinucleotide: I152 (= I157), T153 (= T158), P154 (= P159), K179 (= K184), A212 (≠ P217), K213 (≠ V218), F230 (= F235), T231 (= T236), G232 (= G237), S233 (= S238), V236 (≠ I241), W239 (≠ L244), G256 (= G261)

8of1A Structure of aldh5f1 from moss physcomitrium patens in complex with NAD+ in the contracted conformation
57% identity, 99% coverage: 1:484/487 of query aligns to 14:497/505 of 8of1A

query
sites
8of1A

M

M

G

G

H

T

T

M

L

V

T

Q

L

L

Q

N

R

E

P

A

G

G

L

L

L

F

R

K

S

S

A

Q

C

G

L

L

I

I

D

G

G

D

E

K

W

W

T

V

G

D

A

A

A

E

G

N

G

G

A

H

V

T

L

L

M

P

V

V

H

N

N

N

P

P

A

A

T

T

H

G

D

E

V

I

V

L

G

T

T

S

V

V

P

P

R

F

C

M

G

G

A

K

A

R

E

E

T

A

R

E

Q

K

A

A

V

I

E

A

A

A

A

S

R

Q

A

A

L

F

P

T

A

S

W

W

R

S

D

K

L

R

T

T

G

A

K

N

A

D

R

R

A

S

A

K

V

I

L

L

R

R

R

Q

W

W

A

F

D

N

L

L

M

L

L

I

V

K

H

N

Q

K

E

D

D

D

L

L

A

G

Q

K

L

L

M

I

T

V

A

L

E

E

Q

Q

G

G

K

K

P

P

L

L

A

A

E

E

A

A

R

V

G

G

E

E

I

I

A

V

Y

Y

A

G

A

A

S

A

F

F

L

V

E

E

W

Y

F

Y

G

A

E

E

A

A

K

K

R

R

V

V

D

Y

G

G

D

D

V

I

L

I

S

P

S

S

P

P

R

F

A

P

G

E

Q

K

K

R

M

M

L

L

V

V

L

M

R

K

Q

Q

P

P

V

V

G

G

V

V

C

V

A

A

I
|
I

T
x
A

P
|
P

W
|
W

N

N

F

F

P

P

A

L

A

A

M

M

I

I

T

T

R

R

K

K

A

V

G

A

P

P

A

A

L

L

A

A

G

G

C

C

T

T

M

V

I

V

V

I

K
|
K

P

P

A

S

E

E

Q

L

T

T

P

P

L

L

T

T

A

A

L

L

A

A

L

A

A

A

A

E

L

L

A

A

E

L

E

Q

A

A

G

G

V

I

P

P

R

P

G

G

V

V

L

V

Q

N

V

V

T

M

G

G

D

D

P
x
A

V

K

Q

G

I

I

G

G

G

D

V

A

L

M

C

L

E

D

S

S

P

T

V

E

V

V

R

R

K

K

L

I

S

T

F
|
F

T

T

G
|
G

S
|
S

T

T

A

G

I
x
V

G

G

K

K

L
x
M

L

L

M

L

A

A

Q

G

C

A

A

G

G

K

T

T

V

V

K

K

L

V

S

S

L

L

E

E

L
|
L

G

G

N

N

A

A

P

P

L

C

I

I

V

V

F

F

E

D

D

D

A

A

D

N

L

L

D

D

R

V

A

A

V

V

D

K

G

G

I

V

L

L

A

A

S

G

K

K

F

Y

R

R

N

N

S

S

G

G

Q

Q

T

T

C
|
C

V

V

C

C

A

I

N

N

R

K

I

I

Y

F

V

V

H

Q

D

D

A

G

V

I

Y

Y

D

D

E

K

V

F

A

A

S

E

R

A

L

F

V

A

R

K

A

A

V

V

S

S

A

G

L

L

R

R

P

A

G

G

H

N

G

G

I

L

D

E

S

P

D

G

V

I

T

T

Q

Q

G

G

P

P

L

L

I

I

D

N

A

E

D

T

A

A

V

L

A

E

K
|
K

V

V

E

E

S

R

H

H

I

V

A

Q

D

D

A

A

L

V

A

S

H

K

G

G

A

A

R

K

V

V

L

L

T

V

G

G

K

K

R

R

H

H

A

S

L

L

G

G

G

R

T

T

F

F

F

Y

E

E

P

P

T

T

V

I

V

L

A

G

G

N

A

A

T

S

A

D

A

E

M

M

R

L

V

I

A

F

R

R

E

E

E
|
E

T

V

F
|
F

G

G

P

P

L

V

A

A

P

P

L

L

F

V

R

R

F

F

K

N

G

T

D

D

Q

E

E

E

V

A

I

I

A

K

M

L

A

A

N

N

D

N

T

S

E

E

S

F

G

G

L

L

A

A

Y

Y

F

A

F

F

S

T

K

E

D

N

M

I

A

T

R

R

V

G

W

W

R

R

V

V

A

A

E

E

A

S

L

L

E

E

Y

F

G

G

M

M

V

V

G

G

I

L

N

N

T

E

G

G

L

L

I

I

S

S

N

T

E

E

V

V

A

A

P

P

F

F

G

G

V

M

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

S

K

Y

Y

G

G

I

L

D

D

E

E

Y

Y

L

L

E

E

M

M

K

K

Y

Y

L

V

C

C

L

L

binding nicotinamide-adenine-dinucleotide: I170 (= I157), A171 (≠ T158), P172 (= P159), W173 (= W160), K197 (= K184), A230 (≠ P217), F248 (= F235), G250 (= G237), S251 (= S238), V254 (≠ I241), M257 (≠ L244), L273 (= L260), C306 (= C293), K356 (= K343), E403 (= E390), F405 (= F392)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
54% identity, 97% coverage: 12:483/487 of query aligns to 58:531/535 of P51649

query
sites
P51649

L

L

R

R

S

T

A

D

C

S

L

F

I

V

D

G

G

G

E

R

W

W

T

L

G

P

A

A

G

-

A

A

V

T

L

F

M

P

V

V

H

Q

N

D

P

P

A

A

T

S

H

G

D

A

V

A

V

L

G

G

T

M

V

V

P

A

R

D

C
|
C

G

G

A

V

A

R

E

E

T

A

R

R

Q

A

A

A

V

V

E

R

A

A

A

Y

R

E

A

A

L

F

P

C

A

R

W

W

R

R

D

E

L

V

T

S

G

A

K

K

A

E

R

R

A

S

V

L

L

L

R

R

R

K

W

W

A

Y

D

N

L

L

M

M

L

I

V

Q

H

N

Q

K

E

D

D

D

L

L

A

A

Q

R

L

I

M

I

T

T

A

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

R

H

G

G

E

E

I

I

A

L

Y

Y

A

S

A

A

S

F

F

F

L

L

E

E

W

W

F

F

G

S

E

E

A

A

K

R

R

R

V

V

D

Y

G
|
G

D

D

V

I

L

I

S
x
H

S

T

P
|
P

R

A

A

K

G

D

Q

R

K

R

M

A

L

L

V

V

L

L

R

K

Q

Q

P

P

V

I

G

G

V

V

C

A

A

A

A

V

I

I

T

T

P

P

W

W

N

N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C
|
C

T

T

M

V

I

V

V

V

K
|
K

P
|
P

A
|
A

E
|
E

Q

D

T
|
T

P

P

L

F

T

S

A
|
A

L

L

A

A

L

L

A

A

A

E

L

L

A

A

E

S

E

Q

A

A

G

G

V

I

P

P

R

S

G

G

V

V

L

Y

Q
x
N

V

V

V

I

T

P

-

C

-

S

-

R

G

K

D

N

P

A

V

K

Q

E

I

V

G
|
G

G

E

V

A

L

I

C

C

E

T

S

D

P

P

V

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G
|
G

S
|
S

T
|
T

A
x
T

I
x
T

G
|
G

K

K

L

I

L

L

M

L

A

H

Q

H

C

A

A

A

G

N

T

S

V

V

K

K

K

R

L

V

S

S

L

M

E

E

L

L

G

G

N

L

A

A

P

P

L

F

I

I

V

V

F

F

E

D

D

S

A

A

D

N

L

V

D

D

R

Q

A

A

V

V

D

A

G

G

I

A

L

M

A

A

S

S

K

K

F

F

R
|
R

N
|
N

S

T

G

G

Q

Q

T

T

C
|
C

V

V

C
|
C

A

S

N

N

R

Q

I

F

Y

L

V

V

H

Q

D

R

A

G

V

I

Y

H

D

D

E

A

V

F

A

V

S

K

R

A

L

F

V

A

R

E

A

A

V

M

S

K

A

K

-

N

L

L

R

R

P

V

G

G

H
x
N

G

G

I

F

D

E

S

E

D

G

V

T

T

T

Q

Q

G

G

P
|
P

L

L

I

I

D

N

A

E

D

K

A

A

V

V

A

E

K

K

V

V

E

E

S

K

H

Q

I

V

A

N

D

D

A

A

L

V

A

S

H

K

G

G

A

A

R

T

V
|
V

L

V

T

T

G
|
G

G

G

K

K

R

R

H

H

A

Q

L

L

G

G

G

K

T

N

F

F

E

E

P

P

T

T

V

L

A

C

G

N

A

V

T

T

A

Q

A

D

M

M

R

L

V

C

A

T

R

H

E

E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

R

K

F

F

K

D

G

T

D

E

Q

E

E

E

V

A

I

I

A

A

M

I

A

A

N

N

D

A

T

A

E

D

S

V

G

G

L

L

A

A

A

G

Y

Y

F

F

F

Y

S

S

K

Q

D

D

M

P

A

A

R

Q

V

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

I

V

N

N

T

E

G

G

L

L

I

I

S
|
S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

S

K

Y

Y

G

G

I

I

D

D

E

E

Y

Y

L

L

E

E

M

L

K

K

Y

Y

L

V

C

C

C93 (= C49) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G132) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ S136) to Y: 83% of activity; dbSNP:rs2760118
P182 (= P138) to L: 48% of activity; dbSNP:rs3765310
R213 (= R169) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C179) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (= KPAE 184:187) binding NAD(+)
T233 (= T189) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A193) to S: 65% of activity; dbSNP:rs62621664
N255 (≠ Q211) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G221) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTAIG 237:242) binding NAD(+)
R334 (= R287) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (= N288) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C293) modified: Disulfide link with 342, In inhibited form
C342 (= C295) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (≠ H324) natural variant: N -> S
P382 (= P334) to L: in SSADHD; 2% of activity
V406 (= V358) to I: in dbSNP:rs143741652
G409 (= G361) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (= S450) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832
533 G → R: in SSADHD; <1% of activity; dbSNP:rs72552284

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
54% identity, 97% coverage: 12:483/487 of query aligns to 8:481/485 of 2w8rA

query
sites
2w8rA

L

L

R

R

S

T

A

D

C

S

L

F

I

V

D

G

G

G

E

R

W

W

T

L

G

P

A

A

G

-

A

A

V

T

L

F

M

P

V

V

H

Q

N

D

P

P

A

A

T

S

H

G

D

A

V

A

V

L

G

G

T

M

V

V

P

A

R

D

C

C

G

G

A

V

A

R

E

E

T

A

R

R

Q

A

A

A

V

V

E

R

A

A

A

Y

R

E

A

A

L

F

P

C

A

R

W

W

R

R

D

E

L

V

T

S

G

A

K

K

A

E

R

R

A

S

V

L

L

L

R

R

R

K

W

W

A

Y

D

N

L

L

M

M

L

I

V

Q

H

N

Q

K

E

D

D

D

L

L

A

A

Q

R

L

I

M

I

T

T

A

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

R

H

G

G

E

E

I

I

A

L

Y

Y

A

S

A

A

S

F

F

F

L

L

E

E

W

W

F

F

G

S

E

E

A

A

K

R

R

R

V

V

D

Y

G

G

D

D

V

I

L

I

S

H

S

T

P

P

R

A

A

K

G

D

Q

R

K

R

M

A

L

L

V

V

L

L

R

K

Q

Q

P

P

V

I

G

G

V

V

C

A

A

A

A

V

I

I

T
|
T

P

P

W

W

N
|
N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R

R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C

C

T

T

M

V

I

V

V

V

K
|
K

P

P

A

A

E

E

Q

D

T

T

P

P

L

F

T

S

A

A

L

L

A

A

L

L

A

A

A

E

L

L

A

A

E

S

E

Q

A

A

G

G

V

I

P

P

R

S

G

G

V

V

L

Y

Q

N

V

V

V

I

-

P

-

C

-

S

T

R

G

K

D

N

P
x
A

V

K

Q

E

I

V

G

G

G

E

V

A

L

I

C

C

E

T

S

D

P

P

V

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G

G

S
|
S

T

T

A

T

I
x
T

G

G

K

K

L

I

L

L

M

L

A

H

Q

H

C

A

A

A

G

N

T

S

V

V

K

K

K

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

L

F

I

I

V

V

F

F

E

D

D

S

A

A

D

N

L

V

D

D

R

Q

A

A

V

V

D

A

G

G

I

A

L

M

A

A

S

S

K

K

F

F

R

R

N

N

S

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

I

F

Y

L

V

V

H

Q

D

R

A

G

V

I

Y

H

D

D

E

A

V

F

A

V

S

K

R

A

L

F

V

A

R

E

A

A

V

M

S

K

A

K

-

N

L

L

R

R

P

V

G

G

H

N

G

G

I

F

D

E

S

E

D

G

V

T

T

T

Q

Q

G

G

P

P

L

L

I

I

D

N

A

E

D

K

A

A

V

V

A

E

K

K

V

V

E

E

S

K

H

Q

I

V

A

N

D

D

A

A

L

V

A

S

H

K

G

G

A

A

R

T

V

V

L

V

T

T

G

G

K

K

R

R

H

H

A

Q

L

L

G

G

G

K

T

N

F

F

E

E

P

P

T

T

V

L

A

C

G

N

A

V

T

T

A

Q

A

D

M

M

R

L

V

C

A

T

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

R

K

F

F

K

D

G

T

D

E

Q

E

E

E

V

A

I

I

A

A

M

I

A

A

N

N

D

A

T

A

E

D

S

V

G

G

L

L

A

A

A

G

Y

Y

F

F

F

Y

S

S

K

Q

D

D

M

P

A

A

R

Q

V

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

I

V

N

N

T

E

G

G

L

L

I

I

S

S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

S

K

Y

Y

G

G

I

I

D

D

E

E

Y

Y

L

L

E

E

M

L

K

K

Y

Y

L

V

C

C

active site: N155 (= N161), K178 (= K184), E256 (= E259), A290 (≠ C293), E388 (= E390), E465 (= E467)
binding adenosine-5'-diphosphate: T152 (= T158), K178 (= K184), A214 (≠ P217), S235 (= S238), T238 (≠ I241)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
54% identity, 97% coverage: 12:483/487 of query aligns to 8:481/485 of 2w8qA

query
sites
2w8qA

L

L

R

R

S

T

A

D

C

S

L

F

I

V

D

G

G

G

E

R

W

W

T

L

G

P

A

A

G

-

A

A

V

T

L

F

M

P

V

V

H

Q

N

D

P

P

A

A

T

S

H

G

D

A

V

A

V

L

G

G

T

M

V

V

P

A

R

D

C

C

G

G

A

V

A

R

E

E

T

A

R

R

Q

A

A

A

V

V

E

R

A

A

A

Y

R

E

A

A

L

F

P

C

A

R

W

W

R

R

D

E

L

V

T

S

G

A

K

K

A

E

R

R

A

S

V

L

L

L

R

R

R

K

W

W

A

Y

D

N

L

L

M

M

L

I

V

Q

H

N

Q

K

E

D

D

D

L

L

A

A

Q

R

L

I

M

I

T

T

A

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

R

H

G

G

E

E

I

I

A

L

Y

Y

A

S

A

A

S

F

F

F

L

L

E

E

W

W

F

F

G

S

E

E

A

A

K

R

R

R

V

V

D

Y

G

G

D

D

V

I

L

I

S

H

S

T

P

P

R

A

A

K

G

D

Q

R

K

R

M

A

L

L

V

V

L

L

R

K

Q

Q

P

P

V

I

G

G

V

V

C

A

A

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C

C

T

T

M

V

I

V

V

V

K
|
K

P

P

A

A

E

E

Q

D

T

T

P

P

L

F

T

S

A

A

L

L

A

A

L

L

A

A

A

E

L

L

A

A

E

S

E

Q

A

A

G

G

V

I

P

P

R

S

G

G

V

V

L

Y

Q

N

V

V

V

I

-

P

-

C

-

S

T

R

G

K

D

N

P

A

V

K

Q

E

I

V

G

G

G

E

V

A

L

I

C

C

E

T

S

D

P

P

V

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G

G

S

S

T

T

A

T

I

T

G

G

K

K

L

I

L

L

M

L

A

H

Q

H

C

A

A

A

G

N

T

S

V

V

K

K

K

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

L

F

I

I

V

V

F

F

E

D

D

S

A

A

D

N

L

V

D

D

R

Q

A

A

V

V

D

A

G

G

I

A

L

M

A

A

S

S

K

K

F

F

R
|
R

N

N

S

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

I

F

Y

L

V

V

H

Q

D

R

A

G

V

I

Y

H

D

D

E

A

V

F

A

V

S

K

R

A

L

F

V

A

R

E

A

A

V

M

S

K

A

K

-

N

L

L

R

R

P

V

G

G

H

N

G

G

I

F

D

E

S

E

D

G

V

T

T

T

Q

Q

G

G

P

P

L

L

I

I

D

N

A

E

D

K

A

A

V

V

A

E

K

K

V

V

E

E

S

K

H

Q

I

V

A

N

D

D

A

A

L

V

A

S

H

K

G

G

A

A

R

T

V

V

L

V

T

T

G

G

K

K

R

R

H

H

A

Q

L

L

G

G

G

K

T

N

F

F

E

E

P

P

T

T

V

L

A

C

G

N

A

V

T

T

A

Q

A

D

M

M

R

L

V

C

A

T

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

R

K

F

F

K

D

G

T

D

E

Q

E

E

E

V

A

I

I

A

A

M

I

A

A

N

N

D

A

T

A

E

D

S

V

G

G

L

L

A

A

A

G

Y

Y

F

F

F

Y

S

S

K

Q

D

D

M

P

A

A

R

Q

V

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

I

V

N

N

T

E

G

G

L

L

I

I

S
|
S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

S

K

Y

Y

G

G

I

I

D

D

E

E

Y

Y

L

L

E

E

M

L

K

K

Y

Y

L

V

C

C

active site: N155 (= N161), K178 (= K184), E256 (= E259), A290 (≠ C293), E388 (= E390), E465 (= E467)
binding succinic acid: R163 (= R169), R284 (= R287), A290 (≠ C293), S448 (= S450)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
42% identity, 97% coverage: 15:486/487 of query aligns to 5:476/476 of 5x5uA

query
sites
5x5uA

S

T

A

Q

C

L

L

L

I

I

D

D

G

G

E

E

W

W

T

V

G
x
D

A
|
A

G

S

G
|
G

A

K

V

T

L

I

M

D

V

V

H

V

N

N

P

P

A

A

T

T

H

G

D

K

V

P

V

I

G

G

T

R

V

V

P

A

R

H

C

A

G

G

A

I

A

A

E

D

T

L

R

D

Q

R

A

A

V

L

E

A

A

A

A

Q

R

S

A

G

L

F

P

E

A

A

W

W

R

R

D

K

L

V

T

P

G

A

K

H

A

E

R

R

A

A

V

T

L

M

R

R

R

K

W

A

A

A

D

A

L

L

M

V

L

R

V

E

H

R

Q

A

E

D

D

A

L

I

A

A

Q

Q

L

L

M

M

T

T

A

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

R

R

G

V

E

E

I

V

A

L

Y

S

A

A

S

D

F

I

L

I

E

E

W

W

F

F

G

A

E

D

E

E

A

G

K

R

R

R

V

V

D

Y

G

G

D

R

V

I

L

V

S

P

P

R

R

N

A

L

G

G

Q

A

K

Q

M

Q

L

T

V

V

L

V

R

K

Q

E

P

P

V

V

G

G

V

P

C

V

A

A

I

F

T

T

P
|
P

W

W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

G

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

F

I

L

V

V

K
|
K

P

A

A

P

E

E

Q

E

T

T

P

P

L

A

T

S

A

P

L

A

A

A

L

L

A

L

A

R

L

A

A

F

E

V

E

D

A

A

G

G

V

V

P

P

R

A

G

G

V

V

L

I

Q

G

V

L

V

V

T

Y

G

G

D

D

P
|
P

V
x
A

Q

E

I

I

G
x
S

G

S

V

Y

L

L

C

I

E

P

S

H

P

P

V

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G
|
G

S
|
S

T

T

A

P

I
x
V

G

G

K

K

L

Q

L

L

M

A

A

S

Q

L

C

A

A

G

G

L

T

H

V

M

K

K

K

R

L

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

L

V

I

I

V

V

F

A

E

E

D

D

A

A

D

D

L

V

D

A

R

L

A

A

V

V

D

K

G

A

I

A

L

G

A

G

S

A

K

K

F

F

R

R

N

N

S

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

A

P

N

T

R

R

I

F

Y

L

V

V

H

H

D

N

A

S

V

I

Y

R

D

D

E

E

V

F

A

T

S

R

R

A

L

L

V

V

R

K

A

H

V

A

S

E

A

G

L

L

R

K

P

V

G

G

H

N

G

G

I

L

D

E

S

E

D

G

V

T

T

T

Q

L

G

G

P

A

L

L

I

A

D

N

A

P

D
x
R

A
x
R

V

L

A

T

K

A

V

M

E

A

S

S

H

V

I

I

A

D

D

N

A

A

L

R

A

K

H

V

G

G

A

A

R

S

V

I

L

E

T

T

G

G

K

E

R

R

H

I

A

G

L

S

G

E

G

G

T

N

F

F

E

A

P

P

T

T

V

V

V

I

A

A

G

N

A

V

T

P

A

L

A

D

M

A

R

D

V

V

A

F

R

N

E

N

E
|
E

T

P

F
|
F

G

G

P

P

L

V

A

A

P

A

L

I

F

R

R

G

F

F

K

D

G

K

D

L

Q

E

E

E

V

A

I

I

A

A

M

E

A

A

N

N

D

R

T

L

E

P

S

F

G

G

L

L

A

A

A

G

Y

Y

F

A

F

F

S

T

K

R

D

S

M

F

A

A

R

N

V

V

W

H

R

L

V

L

A

T

E

Q

A

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

I

I

N

N

T

Q

G

P

L

A

I

T

S

P

N

W

E

P

V

E

A

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L

Y

G

G

R

S

E
|
E

G

G

S

G

S

P

Y

E

G

A

I

L

D

E

E

P

Y

Y

L

L

E

V

M

T

K

K

Y

S

L

V

C

T

L

V

E

M

A

A

active site: N151 (= N161), K174 (= K184), E249 (= E259), C283 (= C293), E380 (= E390), E457 (= E467)
binding glycerol: D15 (≠ G25), A16 (= A26), A17 (= A27), G19 (= G29)
binding nicotinamide-adenine-dinucleotide: P149 (= P159), P207 (= P217), A208 (≠ V218), S211 (≠ G221), G227 (= G237), S228 (= S238), V231 (≠ I241), R329 (≠ D339), R330 (≠ A340), E380 (= E390), F382 (= F392)

5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
42% identity, 97% coverage: 15:486/487 of query aligns to 5:476/476 of 5x5tA

query
sites
5x5tA

S

T

A

Q

C

L

L

L

I

I

D

D

G

G

E

E

W

W

T

V

G

D

A

A

G

S

G

G

A

K

V

T

L

I

M

D

V

V

H

V

N

N

P

P

A

A

T

T

H

G

D

K

V

P

V

I

G

G

T

R

V

V

P

A

R

H

C

A

G

G

A

I

A

A

E

D

T

L

R

D

Q

R

A

A

V

L

E

A

A

A

A

Q

R

S

A

G

L

F

P

E

A

A

W

W

R

R

D

K

L

V

T

P

G

A

K

H

A

E

R

R

A

A

V

T

L

M

R

R

R

K

W

A

A

A

D

A

L

L

M

V

L

R

V

E

H

R

Q

A

E

D

D

A

L

I

A

A

Q

Q

L

L

M

M

T

T

A

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

R

R

G

V

E

E

I

V

A

L

Y

S

A

A

S

D

F

I

L

I

E

E

W

W

F

F

G

A

E

D

E

E

A

G

K

R

R

R

V

V

D

Y

G

G

D

R

V

I

L

V

S

P

P

R

R

N

A

L

G

G

Q

A

K

Q

M

Q

L

T

V

V

L

V

R

K

Q

E

P

P

V

V

G

G

V

P

C

V

A

A

I

F

T

T

P

P

W

W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

G

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

F

I

L

V

V

K
|
K

P

A

A

P

E

E

Q

E

T

T

P

P

L

A

T

S

A

P

L

A

A

A

L

L

A

L

A

R

L

A

A

F

E

V

E

D

A

A

G

G

V

V

P

P

R

A

G

G

V

V

L

I

Q

G

V

L

V

V

T

Y

G

G

D

D

P

P

V

A

Q

E

I

I

G

S

V

Y

L

L

C

I

E

P

S

H

P

P

V

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G

G

S

S

T

T

A

P

I

V

G

G

K

K

L

Q

L

L

M
x
A

A

S

Q

L

C

A

A

G

G

L

T

H

V

M

K

K

K

R

L

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

L

V

I

I

V

V

F

A

E

E

D

D

A

A

D

D

L

V

D

A

R

L

A

A

V

V

D

K

G

A

I

A

L

G

A

G

S

A

K

K

F

F

R

R

N

N

S

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

A

P

N

T

R

R

I

F

Y

L

V

V

H

H

D

N

A

S

V

I

Y

R

D

D

E

E

V

F

A

T

S

R

R

A

L

L

V

V

R

K

A

H

V

A

S

E

A

G

L

L

R

K

P

V

G

G

H

N

G

G

I

L

D

E

S

E

D

G

V

T

T

T

Q

L

G

G

P

A

L

L

I

A

D

N

A

P

D

R

A

R

V

L

A

T

K

A

V

M

E

A

S

S

H

V

I

I

A

D

D

N

A

A

L

R

A

K

H

V

G

G

A

A

R

S

V

I

L

E

T

T

G

G

K

E

R

R

H

I

A

G

L

S

G

E

G

G

T

N

F

F

E

A

P

P

T

T

V

V

V

I

A

A

G

N

A

V

T

P

A

L

A

D

M

A

R

D

V

V

A

F

R

N

E

N

E
|
E

T

P

F

F

G

G

P

P

L

V

A

A

P

A

L

I

F

R

R

G

F

F

K

D

G

K

D

L

Q

E

E

E

V

A

I

I

A

A

M

E

A

A

N

N

D

R

T

L

E

P

S

F

G

G

L

L

A

A

A

G

Y

Y

F

A

F

F

S

T

K

R

D

S

M

F

A

A

R

N

V

V

W

H

R

L

V

L

A

T

E

Q

A

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

I

I

N

N

T

Q

G

P

L

A

I

T

S

P

N

W

E

P

V

E

A

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L
x
Y

G

G

R

S

E
|
E

G

G

S

G

S

P

Y

E

G

A

I

L

D

E

E

P

Y

Y

L

L

E

V

M

T

K

K

Y

S

L

V

C

T

L

V

E

M

A

A

active site: N151 (= N161), K174 (= K184), E249 (= E259), C283 (= C293), E380 (= E390), E457 (= E467)
binding glycerol: A236 (≠ M246), Y454 (≠ L464)

6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
38% identity, 96% coverage: 19:484/487 of query aligns to 6:475/477 of 6j76A

query
sites
6j76A

I

V

D

G

G

G

E

E

W

W

T

I

G

D

A

A

A

S

G

N

G

G

A

Q

V

T

L

E

M

P

V

V

H

V

N

S

P

P

A

I

T

N

H

E

D

E

V

V

V

L

G

A

T

Y

V

I

P

Q

R

D

C

A

G

D

A

A

A

S

E

D

T

A

R

E

Q

R

A

V

V

L

E

A

A

V

A

A

A

T

R

T

A

A

L

Q

P

K

A

E

W

W

R

A

D

K

L

Q

T

P

G

A

K

R

A

Q

R

R

A

A

A

E

V

V

L

L

R

R

R

K

W

F

A

A

D

Q

L

L

M

I

L

R

V

D

H

N

Q

K

E

Q

D

Y

L

L

A

A

Q

E

L

L

M

L

T

V

A

K

E

E

Q

Q

G

G

K

K

P

L

L

L

A

K

E

V

A

A

R

L

G

G

E

E

I

V

A

E

Y

A

A

T

A

S

S

T

F

F

L

I

E

E

W

Y

F

A

G

C

E

D

E

W

A

A

K

R

R

Q

V

M

D

D

G

G

D

D

V

I

L

V

S

K

S

S

P

D

R

N

A

A

G

N

Q

E

K

Q

M

I

L

M

V

I

L

H

R

K

Q

I

P

P

V

R

G

G

V

V

C

V

A

V

A

A

I
|
I

T
|
T

P
x
A

W
|
W

N
|
N

F

F

P

P

A

L

A

A

M

L

I

A

T

G

R

R

K

K

A

I

G

G

P

P

A

A

L

L

A

V

A

A

G

G

C

N

T

S

M

I

I

V

V

V

K
|
K

P

P

A
x
T

E
x
S

Q

E

T

T

P

P

L

L

T

A

A

T

L

L

A

E

L

L

A

G

A

Y

L

L

A

A

E

E

E

Q

A

A

G

G

V

I

P

P

R

A

G

G

V

V

L

L

Q

N

V

I

V

V

T

T

G

G

D

G

P
x
G

V

R

Q

T

I

L