SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS26155 FitnessBrowser__Cup4G11:RR42_RS26155 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
65% identity, 99% coverage: 5:484/487 of query aligns to 1:480/482 of P25526

query
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P25526

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WN 156:157 (= WN 160:161) binding
KPAS 180:183 (≠ KPAE 184:187) binding
GS 233:234 (= GS 237:238) binding
L256 (= L260) binding
E386 (= E390) binding

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
65% identity, 98% coverage: 7:484/487 of query aligns to 2:479/481 of 3jz4A

query
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active site: N156 (= N161), K179 (= K184), E254 (= E259), C288 (= C293), E385 (= E390), E462 (= E467)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I152 (= I157), P154 (= P159), W155 (= W160), N156 (= N161), K179 (= K184), A181 (= A186), S182 (≠ E187), S211 (≠ D216), A212 (≠ P217), G213 (≠ V218), G216 (= G221), F230 (= F235), T231 (= T236), G232 (= G237), S233 (= S238), I236 (= I241), E254 (= E259), L255 (= L260), C288 (= C293), K338 (= K343), E385 (= E390), F387 (= F392)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
54% identity, 97% coverage: 12:483/487 of query aligns to 58:531/535 of P51649

query
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P51649

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M

S

K

A

K

-

N

L

L

R

R

P

V

G

G

H
x
N

G

G

I

F

D

E

S

E

D

G

V

T

T

T

Q

Q

G

G

P
|
P

L

L

I

I

D

N

A

E

D

K

A

A

V

V

A

E

K

K

V

V

E

E

S

K

H

Q

I

V

A

N

D

D

A

A

L

V

A

S

H

K

G

G

A

A

R

T

V
|
V

L

V

T

T

G
|
G

G

G

K

K

R

R

H

H

A

Q

L

L

G

G

G

K

T

N

F

F

E

E

P

P

T

T

V

L

A

C

G

N

A

V

T

T

A

Q

A

D

M

M

R

L

V

C

A

T

R

H

E

E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

R

K

F

F

K

D

G

T

D

E

Q

E

E

E

V

A

I

I

A

A

M

I

A

A

N

N

D

A

T

A

E

D

S

V

G

G

L

L

A

A

A

G

Y

Y

F

F

F

Y

S

S

K

Q

D

D

M

P

A

A

R

Q

V

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

I

V

N

N

T

E

G

G

L

L

I

I

S
|
S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

S

K

Y

Y

G

G

I

I

D

D

E

E

Y

Y

L

L

E

E

M

L

K

K

Y

Y

L

V

C

C

C93 (= C49) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G132) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ S136) to Y: 83% of activity; dbSNP:rs2760118
P182 (= P138) to L: 48% of activity; dbSNP:rs3765310
R213 (= R169) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C179) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (= KPAE 184:187) binding
T233 (= T189) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A193) to S: 65% of activity; dbSNP:rs62621664
N255 (≠ Q211) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G221) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTAIG 237:242) binding
R334 (= R287) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (= N288) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C293) modified: Disulfide link with 342, In inhibited form
C342 (= C295) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (≠ H324) natural variant: N -> S
P382 (= P334) to L: in SSADHD; 2% of activity
V406 (= V358) to I: in dbSNP:rs143741652
G409 (= G361) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (= S450) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832
533 G → R: in SSADHD; <1% of activity; dbSNP:rs72552284

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
54% identity, 97% coverage: 12:483/487 of query aligns to 8:481/485 of 2w8rA

query
sites
2w8rA

L

L

R

R

S

T

A

D

C

S

L

F

I

V

D

G

G

G

E

R

W

W

T

L

G

P

A

A

G

-

A

A

V

T

L

F

M

P

V

V

H

Q

N

D

P

P

A

A

T

S

H

G

D

A

V

A

V

L

G

G

T

M

V

V

P

A

R

D

C

C

G

G

A

V

A

R

E

E

T

A

R

R

Q

A

A

A

V

V

E

R

A

A

A

Y

R

E

A

A

L

F

P

C

A

R

W

W

R

R

D

E

L

V

T

S

G

A

K

K

A

E

R

R

A

S

V

L

L

L

R

R

R

K

W

W

A

Y

D

N

L

L

M

M

L

I

V

Q

H

N

Q

K

E

D

D

D

L

L

A

A

Q

R

L

I

M

I

T

T

A

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

R

H

G

G

E

E

I

I

A

L

Y

Y

A

S

A

A

S

F

F

F

L

L

E

E

W

W

F

F

G

S

E

E

A

A

K

R

R

R

V

V

D

Y

G

G

D

D

V

I

L

I

S

H

S

T

P

P

R

A

A

K

G

D

Q

R

K

R

M

A

L

L

V

V

L

L

R

K

Q

Q

P

P

V

I

G

G

V

V

C

A

A

A

A

V

I
|
I

T
|
T

P
|
P

W
|
W

N
|
N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R

R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C

C

T

T

M

V

I

V

V

V

K
|
K

P
|
P

A
|
A

E
|
E

Q

D

T

T

P

P

L

F

T

S

A

A

L

L

A

A

L

L

A

A

A

E

L

L

A

A

E

S

E

Q

A

A

G

G

V

I

P

P

R

S

G

G

V

V

L

Y

Q

N

V

V

V

I

-

P

-

C

-

S

T

R

G

K

D

N

P
x
A

V
x
K

Q

E

I

V

G

G

G

E

V

A

L

I

C

C

E

T

S

D

P

P

V

L

V

V

R

S

K

K

L

I

S

S

F
|
F

T

T

G

G

S
|
S

T

T

A

T

I
x
T

G

G

K

K

L

I

L

L

M

L

A

H

Q

H

C

A

A

A

G

N

T

S

V

V

K

K

K

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

L

F

I

I

V

V

F

F

E

D

D

S

A

A

D

N

L

V

D

D

R

Q

A

A

V

V

D

A

G

G

I

A

L

M

A

A

S

S

K

K

F

F

R

R

N

N

S

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

I

F

Y

L

V

V

H

Q

D

R

A

G

V

I

Y

H

D

D

E

A

V

F

A

V

S

K

R

A

L

F

V

A

R

E

A

A

V

M

S

K

A

K

-

N

L

L

R

R

P

V

G

G

H

N

G

G

I

F

D

E

S

E

D

G

V

T

T

T

Q

Q

G

G

P

P

L

L

I

I

D

N

A

E

D

K

A

A

V

V

A

E

K

K

V

V

E

E

S

K

H

Q

I

V

A

N

D

D

A

A

L

V

A

S

H

K

G

G

A

A

R

T

V

V

L

V

T

T

G

G

K

K

R

R

H

H

A

Q

L

L

G

G

G

K

T

N

F

F

E

E

P

P

T

T

V

L

A

C

G

N

A

V

T

T

A

Q

A

D

M

M

R

L

V

C

A

T

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

R

K

F

F

K

D

G

T

D

E

Q

E

E

E

V

A

I

I

A

A

M

I

A

A

N

N

D

A

T

A

E

D

S

V

G

G

L

L

A

A

A

G

Y

Y

F

F

F

Y

S

S

K

Q

D

D

M

P

A

A

R

Q

V

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

I

V

N

N

T

E

G

G

L

L

I

I

S

S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

S

K

Y

Y

G

G

I

I

D

D

E

E

Y

Y

L

L

E

E

M

L

K

K

Y

Y

L

V

C

C

active site: N155 (= N161), K178 (= K184), E256 (= E259), A290 (≠ C293), E388 (= E390), E465 (= E467)
binding adenosine-5'-diphosphate: I151 (= I157), T152 (= T158), P153 (= P159), W154 (= W160), K178 (= K184), P179 (= P185), A180 (= A186), E181 (= E187), A214 (≠ P217), K215 (≠ V218), F232 (= F235), S235 (= S238), T238 (≠ I241)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
54% identity, 97% coverage: 12:483/487 of query aligns to 8:481/485 of 2w8qA

query
sites
2w8qA

L

L

R

R

S

T

A

D

C

S

L

F

I

V

D

G

G

G

E

R

W

W

T

L

G

P

A

A

G

-

A

A

V

T

L

F

M

P

V

V

H

Q

N

D

P

P

A

A

T

S

H

G

D

A

V

A

V

L

G

G

T

M

V

V

P

A

R

D

C

C

G

G

A

V

A

R

E

E

T

A

R

R

Q

A

A

A

V

V

E

R

A

A

A

Y

R

E

A

A

L

F

P

C

A

R

W

W

R

R

D

E

L

V

T

S

G

A

K

K

A

E

R

R

A

S

V

L

L

L

R

R

R

K

W

W

A

Y

D

N

L

L

M

M

L

I

V

Q

H

N

Q

K

E

D

D

D

L

L

A

A

Q

R

L

I

M

I

T

T

A

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

R

H

G

G

E

E

I

I

A

L

Y
|
Y

A

S

A

A

S

F

F

F

L

L

E

E

W

W

F

F

G

S

E

E

A

A

K

R

R

R

V

V

D

Y

G

G

D

D

V

I

L

I

S

H

S

T

P

P

R

A

A

K

G

D

Q

R

K

R

M

A

L

L

V

V

L

L

R

K

Q

Q

P

P

V

I

G

G

V

V

C

A

A

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F
|
F

P

P

A

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C

C

T

T

M

V

I

V

V

V

K
|
K

P

P

A

A

E

E

Q

D

T

T

P

P

L

F

T

S

A

A

L

L

A

A

L

L

A

A

A

E

L

L

A

A

E

S

E

Q

A

A

G

G

V

I

P

P

R

S

G

G

V

V

L

Y

Q

N

V

V

V

I

-

P

-

C

-

S

T

R

G

K

D

N

P

A

V

K

Q

E

I

V

G

G

G

E

V

A

L

I

C

C

E

T

S

D

P

P

V

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G

G

S

S

T

T

A

T

I

T

G

G

K

K

L

I

L

L

M

L

A

H

Q

H

C

A

A

A

G

N

T

S

V

V

K

K

K

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

L

F

I

I

V

V

F

F

E

D

D

S

A

A

D

N

L

V

D

D

R

Q

A

A

V

V

D

A

G

G

I

A

L

M

A

A

S

S

K

K

F

F

R
|
R

N

N

S

T

G

G

Q

Q

T

T

C
x
A

V
|
V

C

C

A

S

N

N

R

Q

I

F

Y

L

V

V

H

Q

D

R

A

G

V

I

Y

H

D

D

E

A

V

F

A

V

S

K

R

A

L

F

V

A

R

E

A

A

V

M

S

K

A

K

-

N

L

L

R

R

P

V

G

G

H

N

G

G

I

F

D

E

S

E

D

G

V

T

T

T

Q

Q

G

G

P

P

L

L

I

I

D

N

A

E

D

K

A

A

V

V

A

E

K

K

V

V

E

E

S

K

H

Q

I

V

A

N

D

D

A

A

L

V

A

S

H

K

G

G

A

A

R

T

V

V

L

V

T

T

G

G

K

K

R

R

H

H

A

Q

L

L

G

G

G

K

T

N

F

F

E

E

P

P

T

T

V

L

A

C

G

N

A

V

T

T

A

Q

A

D

M

M

R

L

V

C

A

T

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

R

K

F

F

K

D

G

T

D

E

Q

E

E

E

V

A

I

I

A

A

M

I

A

A

N

N

D

A

T

A

E

D

S

V

G

G

L

L

A

A

A

G

Y

Y

F

F

F

Y

S

S

K

Q

D

D

M

P

A

A

R

Q

V

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

I

V

N

N

T

E

G

G

L

L

I

I

S
|
S

N

S

E

V

V

E

A

C

P

P

F
|
F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

S

K

Y

Y

G

G

I

I

D

D

E

E

Y

Y

L

L

E

E

M

L

K

K

Y

Y

L

V

C

C

active site: N155 (= N161), K178 (= K184), E256 (= E259), A290 (≠ C293), E388 (= E390), E465 (= E467)
binding succinic acid: Y109 (= Y115), F156 (= F162), R163 (= R169), E256 (= E259), R284 (= R287), A290 (≠ C293), V291 (= V294), S448 (= S450), F454 (= F456)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
42% identity, 97% coverage: 15:486/487 of query aligns to 5:476/476 of 5x5uA

query
sites
5x5uA

S

T

A

Q

C

L

L

L

I

I

D

D

G

G

E

E

W

W

T

V

G
x
D

A
|
A

G

S

G
|
G

A

K

V

T

L

I

M

D

V

V

H

V

N

N

P

P

A

A

T

T

H

G

D

K

V

P

V

I

G

G

T

R

V

V

P

A

R

H

C

A

G

G

A

I

A

A

E

D

T

L

R

D

Q

R

A

A

V

L

E

A

A

A

A

Q

R

S

A

G

L

F

P

E

A

A

W

W

R

R

D

K

L

V

T

P

G

A

K

H

A

E

R

R

A

A

V

T

L

M

R

R

R

K

W

A

A

A

D

A

L

L

M

V

L

R

V

E

H

R

Q

A

E

D

D

A

L

I

A

A

Q

Q

L

L

M

M

T

T

A

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

R

R

G

V

E

E

I

V

A

L

Y

S

A

A

S

D

F

I

L

I

E

E

W

W

F

F

G

A

E

D

E

E

A

G

K

R

R

R

V

V

D

Y

G

G

D

R

V

I

L

V

S

P

P

R

R

N

A

L

G

G

Q

A

K

Q

M

Q

L

T

V

V

L

V

R

K

Q

E

P

P

V

V

G

G

V

P

C

V

A

A

I

F

T

T

P
|
P

W
|
W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

G

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

F

I

L

V

V

K
|
K

P

A

A

P

E

E

Q

E

T

T

P

P

L

A

T

S

A

P

L

A

A

A

L

L

A

L

A

R

L

A

A

F

E

V

E

D

A

A

G

G

V

V

P

P

R

A

G

G

V

V

L

I

Q

G

V

L

V

V

T

Y

G

G

D

D

P
|
P

V
x
A

Q

E

I

I

G
x
S

G

S

V

Y

L

L

C

I

E

P

S

H

P

P

V

V

V

I

R

R

K

K

L

V

S

T

F
|
F

T
|
T

G
|
G

S
|
S

T

T

A

P

I
x
V

G

G

K

K

L

Q

L
|
L

M

A

A

S

Q

L

C

A

A

G

G

L

T

H

V

M

K

K

K

R

L

A

S

T

L

M

E
|
E

L
|
L

G

G

N

H

A

A

P

P

L

V

I

I

V

V

F

A

E

E

D

D

A

A

D

D

L

V

D

A

R

L

A

A

V

V

D

K

G

A

I

A

L

G

A

G

S

A

K

K

F

F

R

R

N

N

S

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

A

P

N

T

R

R

I

F

Y

L

V

V

H

H

D

N

A

S

V

I

Y

R

D

D

E

E

V

F

A

T

S

R

R

A

L

L

V

V

R

K

A

H

V

A

S

E

A

G

L

L

R

K

P

V

G

G

H

N

G

G

I

L

D

E

S

E

D

G

V

T

T

T

Q

L

G

G

P

A

L

L

I

A

D

N

A

P

D
x
R

A
x
R

V

L

A

T

K

A

V

M

E

A

S

S

H

V

I

I

A

D

D

N

A

A

L

R

A

K

H

V

G

G

A

A

R

S

V

I

L

E

T

T

G

G

K

E

R

R

H

I

A

G

L

S

G

E

G

G

T

N

F

F

E

A

P

P

T

T

V

V

V

I

A

A

G

N

A

V

T

P

A

L

A

D

M

A

R

D

V

V

A

F

R

N

E

N

E
|
E

T

P

F
|
F

G

G

P

P

L

V

A

A

P

A

L

I

F

R

R

G

F

F

K

D

G

K

D

L

Q

E

E

E

V

A

I

I

A

A

M

E

A

A

N

N

D

R

T

L

E

P

S

F

G

G

L

L

A

A

A

G

Y

Y

F

A

F

F

S

T

K

R

D

S

M

F

A

A

R

N

V

V

W

H

R

L

V

L

A

T

E

Q

A

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

I

I

N

N

T

Q

G

P

L

A

I

T

S

P

N

W

E

P

V

E

A

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L

Y

G

G

R

S

E
|
E

G

G

S

G

S

P

Y

E

G

A

I

L

D

E

E

P

Y

Y

L

L

E

V

M

T

K

K

Y

S

L

V

C

T

L

V

E

M

A

A

active site: N151 (= N161), K174 (= K184), E249 (= E259), C283 (= C293), E380 (= E390), E457 (= E467)
binding glycerol: D15 (≠ G25), A16 (= A26), A17 (= A27), G19 (= G29)
binding nicotinamide-adenine-dinucleotide: P149 (= P159), W150 (= W160), N151 (= N161), P207 (= P217), A208 (≠ V218), S211 (≠ G221), F225 (= F235), T226 (= T236), G227 (= G237), S228 (= S238), V231 (≠ I241), L235 (= L245), E249 (= E259), L250 (= L260), C283 (= C293), R329 (≠ D339), R330 (≠ A340), E380 (= E390), F382 (= F392)

5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
42% identity, 97% coverage: 15:486/487 of query aligns to 5:476/476 of 5x5tA

query
sites
5x5tA

S

T

A

Q

C

L

L

L

I

I

D

D

G

G

E

E

W

W

T

V

G

D

A

A

G

S

G

G

A

K

V

T

L

I

M

D

V

V

H

V

N

N

P

P

A

A

T

T

H

G

D

K

V

P

V

I

G

G

T

R

V

V

P

A

R

H

C

A

G

G

A

I

A

A

E

D

T

L

R

D

Q

R

A

A

V

L

E

A

A

A

A

Q

R

S

A

G

L

F

P

E

A

A

W

W

R

R

D

K

L

V

T

P

G

A

K

H

A

E

R

R

A

A

V

T

L

M

R

R

R

K

W

A

A

A

D

A

L

L

M

V

L

R

V

E

H

R

Q

A

E

D

D

A

L

I

A

A

Q

Q

L

L

M

M

T

T

A

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

R

R

G

V

E

E

I

V

A

L

Y

S

A

A

S

D

F

I

L

I

E

E

W

W

F

F

G

A

E

D

E

E

A

G

K

R

R

R

V

V

D

Y

G

G

D

R

V

I

L

V

S

P

P

R

R

N

A

L

G

G

Q

A

K

Q

M

Q

L

T

V

V

L

V

R

K

Q

E

P

P

V

V

G

G

V

P

C

V

A

A

I

F

T

T

P

P

W

W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

G

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

F

I

L

V

V

K
|
K

P

A

A

P

E

E

Q

E

T

T

P

P

L

A

T

S

A

P

L

A

A

A

L

L

A

L

A

R

L

A

A

F

E

V

E

D

A

A

G

G

V

V

P

P

R

A

G

G

V

V

L

I

Q

G

V

L

V

V

T

Y

G

G

D

D

P

P

V

A

Q

E

I

I

G

S

V

Y

L

L

C

I

E

P

S

H

P

P

V

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G

G

S

S

T

T

A

P

I

V

G

G

K

K

L

Q

L

L

M
x
A

A

S

Q

L

C
x
A

A
x
G

G

L

T

H

V

M

K

K

K

R

L

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

L

V

I

I

V

V

F

A

E

E

D

D

A

A

D

D

L

V

D

A

R

L

A

A

V

V

D

K

G

A

I

A

L

G

A

G

S

A

K

K

F

F

R

R

N

N

S

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

A

P

N

T

R

R

I

F

Y

L

V

V

H

H

D

N

A

S

V

I

Y

R

D

D

E

E

V

F

A

T

S

R

R

A

L

L

V

V

R

K

A

H

V

A

S

E

A

G

L

L

R

K

P

V

G

G

H

N

G

G

I

L

D

E

S

E

D

G

V

T

T

T

Q

L

G

G

P

A

L

L

I

A

D

N

A

P

D

R

A

R

V

L

A

T

K

A

V

M

E

A

S

S

H

V

I

I

A

D

D

N

A

A

L

R

A

K

H

V

G

G

A

A

R

S

V

I

L

E

T

T

G

G

K

E

R

R

H

I

A

G

L

S

G

E

G

G

T

N

F

F

E

A

P

P

T

T

V

V

V

I

A

A

G

N

A

V

T

P

A

L

A

D

M

A

R

D

V

V

A

F

R

N

E

N

E
|
E

T

P

F

F

G

G

P

P

L

V

A

A

P

A

L

I

F

R

R

G

F

F

K

D

G

K

D

L

Q

E

E

E

V

A

I

I

A

A

M

E

A

A

N

N

D

R

T

L

E

P

S

F

G

G

L

L

A

A

A

G

Y

Y

F

A

F

F

S

T

K

R

D

S

M

F

A

A

R

N

V

V

W

H

R

L

V

L

A

T

E

Q

A

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

I

I

N

N

T

Q

G

P

L

A

I

T

S

P

N

W

E

P

V

E

A

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L
x
Y

G

G

R

S

E
|
E

G

G

S

G

S

P

Y

E

G

A

I

L

D

E

E

P

Y

Y

L

L

E

V

M

T

K

K

Y

S

L

V

C

T

L

V

E

M

A

A

active site: N151 (= N161), K174 (= K184), E249 (= E259), C283 (= C293), E380 (= E390), E457 (= E467)
binding glycerol: A236 (≠ M246), A239 (≠ C249), G240 (≠ A250), Y454 (≠ L464)

6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
38% identity, 96% coverage: 19:484/487 of query aligns to 6:475/477 of 6j76A

query
sites
6j76A

I

V

D

G

G

G

E

E

W

W

T

I

G

D

A

A

A

S

G

N

G

G

A

Q

V

T

L

E

M

P

V

V

H

V

N

S

P

P

A

I

T

N

H

E

D

E

V

V

V

L

G

A

T

Y

V

I

P

Q

R

D

C

A

G

D

A

A

A

S

E

D

T

A

R

E

Q

R

A

V

V

L

E

A

A

V

A

A

A

T

R

T

A

A

L

Q

P

K

A

E

W

W

R

A

D

K

L

Q

T

P

G

A

K

R

A

Q

R

R

A

A

A

E

V

V

L

L

R

R

R

K

W

F

A

A

D

Q

L

L

M

I

L

R

V

D

H

N

Q

K

E

Q

D

Y

L

L

A

A

Q

E

L

L

M

L

T

V

A

K

E

E

Q

Q

G

G

K

K

P

L

L

L

A

K

E

V

A

A

R

L

G

G

E

E

I

V

A

E

Y

A

A

T

A

S

S

T

F

F

L

I

E

E

W

Y

F

A

G

C

E

D

E

W

A

A

K

R

R

Q

V

M

D

D

G

G

D

D

V

I

L

V

S

K

S

S

P

D

R

N

A

A

G

N

Q

E

K

Q

M

I

L

M

V

I

L

H

R

K

Q

I

P

P

V

R

G

G

V

V

C

V

A

V

A

A

I
|
I

T
|
T

P
x
A

W
|
W

N
|
N

F

F

P

P

A

L

A

A

M

L

I

A

T

G

R

R

K

K

A

I

G

G

P

P

A

A

L

L

A

V

A

A

G

G

C

N

T

S

M

I

I

V

V

V

K
|
K

P
|
P

A
x
T

E
x
S

Q

E

T

T

P

P

L

L

T

A

A

T

L

L

A

E

L

L

A

G

A

Y

L

L

A

A

E

E

E

Q

A

A

G

G

V

I

P

P

R

A

G

G

V

V

L

L

Q

N

V

I

V

V

T

T

G

G

D
x
G

P
x
G

V
x
R

Q

T

I

L

G
|
G

G
x
N

V

E

L

L

C

V

E

G

S

H

P

R

V

M

V

T

R

N

K

M

L

V

S

S

F

M

T
|
T

G
|
G

S
|
S

T

T

A

P

I
x
A

G

G

K

Q

L
x
S

L
x
I

M

I

A

R

Q

A

C

S

A

A

G

N

T

N

V

M

K

A

K

H

L

V

S

Q

L

L

E
|
E

L
|
L

G
|
G

G

G

N

K

A

A

P

P

L

F

I

I

V

V

F

M

E

E

D

D

A

A

D

D

L

L

D

E

R

Q

A

A

V

A

D

A

G

A

I

A

L

L

A

H

S

S

K

R

F

F

R

D

N

N

S

C

G

G

Q

Q

T

V

C
|
C

V

T

C

C

A

N

N

E

R

R

I

M

Y

Y

V

V

H

H

D

G

A

A

V

V

Y

Y

D

D

E

E

V

F

A

M

S

R

R

I

L

F

V

M

R

G

A

K

V

V

S

E

A

A

L

I

R

K

P

V

G

G

H

D

G

P

I

M

D

D

S

P

D

A

V

S

T

D

Q

M

G

G

P

P

L

K

I

V

D

N

A

A

D

N

A

E

V

L

A

A

K

H

V

M

E

E

S

E

H

L

I

V

A

A

D

E

A

A

L

V

A

D

H

E

G

G

A

A

R

T

V

V

L

L

T

F

G

G

K

K

R

K

-

L

-

E

-

G

-

P

H

E

A

F

L

E

G

K

G

G

T

F

F

W

F

F

E

E

P

P

T

T

V

V

V

L

A

T

G

N

A

V

T

T

A

Q

A

D

M

M

R

T

V

I

A

V

R

H

E

E

E
|
E

T

S

F
|
F

G

G

P

P

L

I

A

L

P

P

L

V

F

I

R

K

F

F

K

D

G

S

D

F

Q

D

E

E

V

V

I

I

A

E

M

Y

A

A

N

N

D

D

T

S

E

D

S

Y

G

G

L

L

A

A

Y

M

F

I

F

C

S

T

K

Q

D

N

M

M

A

H

R

Y

V

I

W

N

R

R

V

L

A

L

E

T

A

E

L

L

E

E

Y

S

G

G

M

E

V

I

G

Y

I

V

N

N

T

R

G

G

L

H

I

G

S

E

N

Q

E

H

V

Q

A

G

P

F

F
x
H

G

N

G

G

V

Y

K

K

Q

L

S

S

G

G

L

T

G

G

R

G

E
|
E

G

D

S

G

S

K

Y

Y

G

G

I

F

D

E

E

Q

Y

Y

L

L

E

E

M

K

K

K

Y

T

L

F

C

Y

L

I

active site: N148 (= N161), E246 (= E259), C280 (= C293), E458 (= E467)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I144 (= I157), T145 (= T158), A146 (≠ P159), W147 (= W160), N148 (= N161), K171 (= K184), P172 (= P185), T173 (≠ A186), S174 (≠ E187), G203 (≠ D216), G204 (≠ P217), R205 (≠ V218), G208 (= G221), N209 (≠ G222), T223 (= T236), G224 (= G237), S225 (= S238), A228 (≠ I241), S231 (≠ L244), I232 (≠ L245), E246 (= E259), L247 (= L260), G248 (= G261), C280 (= C293), E381 (= E390), F383 (= F392), H447 (≠ F456)

2opxA Crystal structure of lactaldehyde dehydrogenase from escherichia coli
36% identity, 97% coverage: 13:486/487 of query aligns to 3:477/477 of 2opxA

query
sites
2opxA

L

V

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A

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A

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N

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C

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A

A

A

E

E

D

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A

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A

A

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A

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A

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E

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A

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L

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I

T

V

A

E

E

E

Q

G

G

G

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K

P

I

L

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A
x
Q

E
x
L

A

A

R

E

G
x
V

E

E

I

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A

A

Y
x
F

A

T

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D

F

Y

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Y

F

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A

E

E

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E

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D

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R

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P

G

G

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E

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N

M

I

L

L

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L

L

F

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A

V

L

G

G

V

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C

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A

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A

G

I

I

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L

P

P

W

W

N
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N

F
|
F

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P

A

F

A
x
F

M

L

I

I

T

A

R

R

K

K

A

M

G

A

P

P

A

A

L

L

A

L

A

T

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G

C

N

T

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I

I

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K
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K

P

P

A

S

E

E

Q

F

T

T

P

P

L

N

T

N

A

A

L

I

A

A

L

F

A

A

A

K

L

I

A

V

E

D

E

E

A

I

G

G

V

L

P

P

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R

G

G

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V

L

F

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N

V

L

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L

G

G

D

R

P

G

V

E

Q

T

I

V

G

G

G

Q

V

E

L

L

C

A

E

G

S

N

P

P

V

K

V

V

R

A

K

M

L

V

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S

F

M

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T

G

G

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V

A

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I

A

G

G

K

E

L

K

L

I

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M

A

A

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C

A

A

A

G

K

T

N

V

I

K

T

K

K

L

V

S

C

L

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E
|
E

L

L

G

G

N

K

A

A

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P

L

A

I

I

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V

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M

E

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D

D

A

A

D

D

L

L

D

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R

L

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G

A

I

I

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x
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F

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R
x
I

N

N

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S

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G

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C

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x
N

C

C

A

A

N

E

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Y

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H

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K

A

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Y

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L

V

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R

E

A

A

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S

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A

A

L

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P
x
F

G
|
G

H

N

G

P

I

A

D

E

-
x
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S

N

D
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D

V
x
I

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x
A

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G

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P

L

L

I

I

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A

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A

A

A

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A

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A

A

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E

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L

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A

H

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A

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G

G
x
K

G

G

T

Y

F
x
Y

F

Y

E

P

P

P

T

T

V

L

A

L

G

D

A

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A

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M

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A

M

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E

E

E
|
E

T

T

F

F

G

G

P

P

L

V

A

L

P

P

L

V

F

V

R

A

F

F

K

D

G

T

D

L

Q

E

E

E

V

A

I

I

A

S

M

M

A

A

N

N

D

D

T

S

E

D

S

Y

G

G

L

L

A

T

A

S

Y

S

F

I

F

Y

S

T

K

Q

D

N

M

L

A

N

R

V

V

A

W

M

R

K

V

A

A

I

E

K

A

G

L

L

E

K

Y

F

G

G

M

E

V

T

G

Y

I

I

N

N

T

R

G

E

L

N

I
x
F

S
x
E

N

A

E

M

V

Q

A

G

P

F

F

H

G

A

G

G

V

W

K

R

Q

K

S

S

G

G

L

I

G

G

R

G

E
x
A

G

D

S

G

S

K

Y

H

G

G

I

L

D

H

E

E

Y

Y

L

L

E

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Y

L

L

E

Q

A

S

active site: N151 (= N161), K174 (= K184), E249 (= E259), C283 (= C293), E381 (= E390), A458 (≠ E467)
binding (3alpha,5beta,12alpha)-3,12-dihydroxycholan-24-oic acid: Q97 (≠ A107), L98 (≠ E108), V101 (≠ G111), F105 (≠ Y115), F152 (= F162), F155 (≠ A165), D273 (≠ A283), I277 (≠ R287), N284 (≠ V294), F312 (≠ P322), G313 (= G323), R318 (vs. gap), D320 (= D329), I321 (≠ V330), A322 (≠ T331), K359 (≠ G368), Y362 (≠ F371), F440 (≠ I449), F440 (≠ I449), E441 (≠ S450)

2impA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the ternary complex with lactate (occupancy 0.5) and nadh. Crystals soaked with (l)-lactate. (see paper)
36% identity, 97% coverage: 13:486/487 of query aligns to 3:477/477 of 2impA

query
sites
2impA

L

V

R

Q

S

H

A

P

C

M

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Y

I

I

D

D

G

G

E

Q

W

F

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G

T

A

W

A

R

G

G

G

D

A

A

V

W

L

I

M

D

V

V

H

V

N

N

P

P

A

A

T

T

H

E

D

A

V

V

V

I

G

S

T

R

V

I

P

P

R

D

C

G

G

Q

A

A

A

E

E

D

T

A

R

R

Q

K

A

A

V

I

E

D

A

A

A

E

R

R

A

A

L

Q

P

P

A

E

W

W

R

E

D

A

L

L

T

P

G

A

K

I

A

E

R

R

A

A

A

S

V

W

L

L

R

R

R

K

W

I

A

S

D

A

L

G

M

I

L

R

V

E

H

R

Q

A

E

S

D

E

L

I

A

S

Q

A

L

L

M

I

T

V

A

E

E

E

Q

G

G

G

K

K

P

I

L

Q

A

Q