SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS26915 FitnessBrowser__Cup4G11:RR42_RS26915 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q4WR83 Acyl-CoA ligase sidI; Siderophore biosynthesis protein I; EC 6.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
41% identity, 95% coverage: 17:560/573 of query aligns to 23:571/590 of Q4WR83

query
sites
Q4WR83

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Sites not aligning to the query:

6:14 PTS2-type peroxisomal targeting signal

P69451 Long-chain-fatty-acid--CoA ligase; Long-chain acyl-CoA synthetase; Acyl-CoA synthetase; EC 6.2.1.3 from Escherichia coli (strain K12) (see paper)
32% identity, 92% coverage: 36:560/573 of query aligns to 28:551/561 of P69451

query
sites
P69451

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Y213 (≠ F221) mutation to A: Loss of activity.
T214 (= T222) mutation to A: 10% of wild-type activity.
G216 (= G224) mutation to A: Decreases activity.
T217 (= T225) mutation to A: Decreases activity.
G219 (= G227) mutation to A: Decreases activity.
K222 (= K230) mutation to A: Decreases activity.
E361 (= E367) mutation to A: Loss of activity.

8wevA Crystal structure of feruoyl-coa synthetase complexed with amp from amycolatopsis thermoflava
32% identity, 90% coverage: 48:561/573 of query aligns to 19:486/486 of 8wevA

query
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8wevA

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C

R

V

F

N

I

V

A

L

M

V

A

E

M

T

R

D

F

L

S

A

E

S

Q

D

D

E

K
x
R

L

A

C

L

I

V

P

A

V

A

P

P

F

L

Y

F

H

H

C

A

F

A

G

A

M

L

V

G

L

M

A

V

V

C

L

L

A

P

C

T

V

L

S

L

T

K

G

G

A

G

C

T

M

V

V

I

F

L

P

-

G

H

E

S

A

A

F

F

E

D

P

P

A

G

A

A

T

V

M

L

A

S

A

A

V

V

S

E

E

Q

E

E

R

R

C

V

T

T

A

L

L

V

H

F

G

G

V

V

P

P

T

T

M

M

F

Y

I

Q

A

A

Q

I

L

A

D

A

H

H

P

P

D

R

F

W

A

R

K

S

Y

T

D

D

F

L

S

S

T

S

L

L

R

R

T

T

G

L

I

L

M

C

A

G

G
|
G

S
x
A

P

P

C

V

P

P

I

A

E

D

T

L

M

A

K

G

R

R

V

Y

I

L

S

D

Q

R

M

-

H

G

M

L

A

A

Q

F

V

V

T

Q

I

-

A

G

Y
|
Y

G
|
G

M
|
M

T
|
T

E

E

T

A

S

A

P

P

V

G

S

V

F

L

Q

V

S

L

S

D

T

R

T

A

D

H

P

-

L

V

D

A

K

E

R

K

V

I

T

G

T

S

V

A

G

G

Q

V

I

P

Q

S

P

F

H

F

L

T

E

D

V

V

K

R

L

V

V

A

D

G

G

P

T

S

G

G

E

E

I

P

V

V

P

P

V

P

G

G

E

E

K

K

G

G

E

E

L

I

C

V

T

V

R

S

G

G

Y

P

S

N

V

V

M

M

Q

K

G

G

Y

Y

W

W

D

G

D

R

E

P

P

E

R

A

T

T

R

A

E

E

A

V

I

L

R

R

D

D

G

G

W

W

M

F

H

R

T

S

G

G

D
|
D

L

V

A

A

T

T

I

V

D

D

A

G

E

D

G

G

Y

Y

C

F

N

H

I

V

V

V

G

D

R
|
R

V

L

K

K

D

D

M

M

L

I

I

I

R

S

G

G

E

E

N

N

V

I

Y

Y

P

P

R

A

E

E

I

V

E

E

E

N

F

E

L

L

F

Y

R

G

H

Y

P

P

K

G

V

V

Q

E

A

A

V

C

Q

A

V

V

F

I

G

G

V

V

P

P

D

D

P

P

K

R

Y

W

G

G

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E

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V

V

G

C

K

A

A

W

V

I

V

V

V

L

P

K

A

P

A

G

G

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S

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A

I

T

D

E

G

D

A

D

E

I

L

R

L

A

A

F

W

C

L

R

R

D

T

Q

R

I

L

A

A

H

G

Y

Y

K

K

I

V

P

P

R

K

Y

S

I

V

R

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F

F

V

T

D

D

E

R

M

L

P

P

M

T

T

T

I

G

T

S

G

G

K

K

V

I

Q

L

K

K

F

G

V

E

M

V

R

R

E

R

R

R

binding adenosine monophosphate: G267 (= G339), A268 (≠ S340), Y289 (= Y363), G290 (= G364), M291 (= M365), T292 (= T366), D371 (= D446), R386 (= R461)
binding magnesium ion: R24 (= R53), R186 (≠ K257)

4gxqA Crystal structure of atp bound rpmatb-bxbclm chimera b1 (see paper)
32% identity, 88% coverage: 56:560/573 of query aligns to 26:499/506 of 4gxqA

query
sites
4gxqA

G

G

I

D

R

K

W

I

S

S

W

Y

R

A

E

E

F

L

A

V

Q

A

V

R

V

A

D

G

A

R

M

V

A

A

G

N

G

V

L

L

R

V

L

A

L

R

G

G

L

L

E

Q

R

V

G

G

D

D

R

R

I

V

G

A

I

A

W

Q

S

T

P

E

N

K

R

S

V

V

E

E

W

A

L

L

V

V

T

L

Q

Y

F

L

A

A

T

T

A

V

R

R

L

A

G

G

L

G

V

V

L

Y

V

L

N

P

I

L

N

N

P

T

A

A

Y

Y

R

T

L

L

A

H

E

E

L

L

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D

Y

Y

A

F

L

I

N

T

K

D

V

A

G

E

C

P

K

K

A

I

I

V

V

V

A

-

E

-

S

-

F

-

K

-

T

-

S

-

R

-

Y

-

L

-

E

-

M

-

L

-

Q

-

T

-

L

-

A

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P

-

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L

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K

-

S

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C

C

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D

P

P

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S

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K

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G

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I

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K

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A

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-

W

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V

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G

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M

L

L

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N

D

Y

A

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A

A

A

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G

L

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T

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S

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G

L

A

D

D

A

D

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L

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A

A

A

Q

-

L

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-

Y

-

D

-

P

-

I

-

N

-

I

I

Q

L

F

Y

T
|
T

S
|
S

G
|
G

T
|
T

G

G

A

R

P

S

K

K

G

G

A

A

T

M

L

L

T

S

H

H

R

D

N

N

I

L

V

A

N

S

N
|
N

A

S

R

L

F

T

I

L

A

V

M

D

A

Y

M

W

R

R

F

F

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P

Q

D

D

D

K

V

L

L

C

I

I

H

P

A

V

L

P

P

F

I

Y

Y

H
|
H

C

T

F

H

G

G

M

L

V

F

L

V

A

A

V

S

L

N

A

V

C

T

V

L

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F

T

A

G

R

A

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M

M

V

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F

F

P

L

G

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A

-

F

F

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P

A

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M

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-

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L

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G

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F

F

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I

T

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L

L

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Q

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K

Y

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F

T

S

G

T

H

L

M

R

R

T

L

G

F

I

I

M

S

A

G

G
x
S

S
x
A

P
|
P

C

L

P

L

I

A

E

D

T

T

M

H

K

R

R

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V

W

I

S

S

A

Q

K

M

T

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G

M

H

A

A

Q

-

V

V

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L

I
x
E

A
x
R

Y

Y

G
|
G

M
|
M

T
|
T

E
|
E

T

T

S

N

P

-

V

-

S

-

F

-

Q

-

S

M

S

N

T

T

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N

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P

L

Y

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G

K

D

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T

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A

E

I

F

R

R

D

D

-

D

G

G

W

F

M

F

H

I

T

T

G

G

D
|
D

L

L

A

G

T

K

I

I

D

D

A

E

E

R

G

G

Y

Y

C

V

N

H

I

I

V

L

G

G

R
|
R

V

G

K

K

D

D

M

L

L

V

I
|
I

R

T

G

G

E

F

N
|
N

V

V

Y

Y

P

P

R

K

E

E

I

I

E

E

E

S

F

E

L

I

F

D

R

A

H

M

P

P

K

G

V

V

Q

V

A

E

V

S

Q

A

V

V

F

I

G

G

V

V

P

P

D

H

P

A

K

D

Y

F

G

G

E

E

E

G

V

V

C

T

A

A

W

F

I

V

V

V

L

L

K

K

-

R

-

E

-

F

-

A

P

P

G

S

D

E

T

I

A

L

T

A

E

E

D

-

D

E

I

L

R

K

A

A

F

F

C

V

R

K

D

D

Q

R

I

L

A

A

H

K

Y

F

K

K

I

M

P

P

R

K

Y

K

I

V

R

I

F

F

V

V

D

D

E

D

M

L

P

P

M

R

T

N

I

T

T

M

G

G

K
x
A

V

V

Q

Q

K

K

F

N

V

V

M

L

R

R

E

E

active site: T163 (= T222), N183 (= N242), H207 (= H266), T303 (= T366), E304 (= E367), I403 (= I467), N408 (= N472), A491 (≠ K552)
binding adenosine-5'-triphosphate: T163 (= T222), S164 (= S223), G165 (= G224), T166 (= T225), T167 (= T226), H207 (= H266), S277 (≠ G339), A278 (≠ S340), P279 (= P341), E298 (≠ I361), M302 (= M365), T303 (= T366), D382 (= D446), R397 (= R461)
binding carbonate ion: H207 (= H266), S277 (≠ G339), R299 (≠ A362), G301 (= G364)

P9WQ37 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
29% identity, 91% coverage: 45:563/573 of query aligns to 16:498/503 of P9WQ37

query
sites
P9WQ37

P

P

D
x
R

R

L

E

Q

A

A

V

Y

V

V

F

E

R

P

E

S

Q

T

G

D

I

V

R

R

W

M

S

T

W

Y

R

A

E

Q

F

M

A

N

Q

A

V

L

V

A

D

N

A

R

M

C

A

A

G

D

G

V

L

L

R

T

L

A

L

L

G

G

L

I

E

A

R

K

G

G

D

D

R

R

I

V

G

A

I

L

W

L

S

M

P

P

N

N

R

S

V

V

E

E

W

F

L

C

V

C

T

L

Q

F

F

Y

A

G

T

A

A

A

R

K

L

L

G

G

L

A

V

V

L

A

V

V

N

P

I

I

N

N

P

T

A

R

Y

L

R

A

L

A

A

P

E

E

L

V

E

S

Y

F

A

I

L

L

N

S

K

D

V

S

G

G

C

S

K

K

A

V

I

V

V

I

A

Y

A

G

E

A

S

-

F

-

K

P

T

S

S

A

R

P

Y

V

L

I

E

D

M

A

L

I

Q

R

T

A

L

Q

A

A

P

D

E

P

L

-

K

-

S

-

C

-

E

-

P

P

G

G

T

T

L

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Q

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A

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R

-

L

-

P

-

A

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L

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D

W

W

V

I

I

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-

M

-

G

G

A

A

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D

R

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T

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P

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G

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M

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L

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N

-

Y

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D

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A

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L

-

L

L

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A

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F

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M

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Y

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G

G

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G

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A

H

P

P

K
|
K

G

G

A

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L

H

T

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N

S

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x
R

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D

K

R

L

L

C

L

I

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P

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L

P

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F

M

Y

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H

H

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x
V

F

A

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x
A

M

L

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|
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N

M

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x
A

M

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P

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T

T

R

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A

A

I

F

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D

D

N

G

G

W
|
W

M

F

H

R

T

T

G

G

D
|
D

L

I

A

G

T

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I

I

D

D

A

D

E

E

G

G

Y

Y

C

L

N

Y

I

I

V

K

G

D

R
|
R

V

L

K

K

D

D

M

M

L

I

I

I

R
x
S

G

G

G
|
G

E

E

N

N

V

V

Y

Y

P

P

R

A

E

E

I

I

E

E

E

S

F

V

L

I

F

I

R

G

H

V

P

P

K

G

V

V

Q

S

A

E

V

V

Q

A

V

V

F

I

G

G

V

L

P

P

D

D

P

E

K

K

Y

W

G

G

E

-

E

E

V

I

C

A

A

A

W

A

I

I

V

V

L

V

K

A

P

D

G

Q

D

N

T

E

A

V

T

S

E

E

D

Q

I

I

R

V

A

E

F

Y

C

C

R

G

D

T

Q

R

I

L

A

A

H

R

Y

Y

K

K

I

L

P

P

R

K

Y

K

I

V

R

I

F

F

V

A

D

E

E

A

M

I

P

P

M

R

T

N

I

P

T

T

G

G

K
|
K

V

I

Q

L

K

K

F

T

V

V

M

L

R

R

E

E

R

Q

M

Y

T

S

R17 (≠ D46) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
K172 (= K230) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
R195 (≠ S253) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
R197 (≠ Q255) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
V209 (≠ C267) mutation to D: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
A211 (≠ G269) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
T214 (≠ L272) mutation to W: Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
R244 (= R304) mutation to A: Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
A302 (≠ G364) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.; mutation to W: Does not show activity with small, medium or long acyl chains.
W377 (= W441) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
D382 (= D446) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
R397 (= R461) mutation to A: Reduction of binding affinity for fatty acids.
S404 (≠ R468) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
G406 (= G470) mutation to L: No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
K487 (= K552) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.

Sites not aligning to the query:

9 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.

4wv3B Crystal structure of the anthranilate coa ligase auaeii in complex with anthranoyl-amp (see paper)
30% identity, 95% coverage: 23:568/573 of query aligns to 3:518/518 of 4wv3B

query
sites
4wv3B

S

A

T

S

D

D

I

L

P

P

L

L

S

Y

-

Y

-

N

-

A

-

V

-

D

-

I

-

L

E

E

L

R

T

N

V

L

P

P

A

V

L

R

L

A

A

N

E

K

T

T

V

A

A

L

R

F

F

T

P

P

D

D

R

R

E

E

A

-

V

M

V

T

F

F

R

R

E

Q

Q

V

G

S

I

-

R

-

W

-

S

-

W

-

R

N

E

E

F

A

A

N

Q

Q

V

V

V

G

D

N

A

A

M

L

A

K

G

G

G

-

L

-

R

-

L

-

L

L

G

G

L

V

E

R

R

F

G

G

D

E

R

C

I

V

G

G

I

L

W

L

S

T

P

L

N

D

R

S

V

A

E

E

W

W

L

V

V

T

T

S

Q

F

F

F

A

G

T

I

A

V

R

K

L

L

G

G

L

A

V

I

L

A

V

V

N

G

I

I

N

N

P

T

A

L

Y

L

R

K

L

P

A

P

E

E

L

Y

E

E

Y

Y

A

I

L

L

N

R

K

D

V

-

G

-

C

C

K

R

A

A

I

-

V

-

A

-

E

-

S

-

F

-

K

-

T

-

S

-

R

R

Y

V

L

L

E

I

M

V

L

H

Q

Q

T

E

L

F

A

L

P

P

E

L

L

I

K

E

S

S

C

I

E

R

P

-

G

-

T

-

L

-

Q

-

A

-

A

G

R

N

L

L

P

P

A

M

L

L

R

E

W

H

V

I

I

V

R

V

M

I

G

G

A

E

E

G

R

P

T

Q

P

E

G

G

M

Y

L

L

N

S

Y

F

D

N

A

D

L

W

L

I

A

R

H

P

G

Q

T

P

H

T

A

T

L

L

T

E

S

A

E

-

L

-

D

-

A

-

L

-

T

-

A

A

Q

Q

L

S

D

H

R

R

Y

E

D

D

P

I

I

C

N

S

I

L

Q

N

F

Y

T
x
S

S

S

G

G

T

T

G

G

A

G

P

P

K

K

G

G

A

I

T

P

L

H

T

A

H

H

R

K

N

D

I

Y

V

P

N

L

N

T

A

A

R

Q

F

L

I

W

A

G

M

V

-

N

A

V

M

L

R

G

F

L

S

R

E

E

Q

S

D

D

K

R

L

T

C

F

I

A

P

L

V

A

P

K

F

L

Y

F

H
x
F

C
x
T

F
|
F

G

G

M

T

V

G

L

G

A

N

V

L

L

I

A

F

C

P

V

W

S

Y

T

V

G

G

A

A

-

S

C

C

M

V

V

L

F

F

P

P

G

G

E

A

A

A

F

R

E

V

P

A

A

S

A

N

T

V

M

L

A

S

A

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I

S

S

E

R

E

F

R

K

C

P

T

T

A

I

L

F

H

Y

G

N

V

A

P

P

T

T

M

G

F

Y

I

A

A

A

Q

A

L

L

D

A

H

L

P

K

D

D

F

F

A

S

K

Q

Y

H

D

D

F

L

S

S

T

S

L

L

R

R

T

L

G

C

I

V

M

S

A
|
A

G
x
S

S
x
E

P
x
A

C

L

P

P

I

-

E

A

T

A

M

L

K

W

R

Y

V

A

I

W

S

K

Q

E

M

A

H

T

M

G

A

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Q

D

V

I

T

I

I

D

A
x
G

Y
x
I

G
|
G

M
x
C

T
|
T

E
|
E

T

N

S

F

P

H

V

I

S

F

F

I

Q

-

S

-

T

S

T

N

D

R

P

P

L

G

D

D

K

I

R

R

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G

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S

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G

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K

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Q

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P

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H

G

L

Y

E

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L

K

K

L

L

V

V

D

D

G

D

T

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G

G

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K

I

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P

V

A

G

G

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E

K

I

G

G

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N

L

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C

L

T

L

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R

G

S

Y

E

S

T

V

A

M

A

Q

L

G

S

Y

Y

W

W

D

H

D

N

E

F

P

E

R

K

T

S

R

R

E

Q

A

T

I

F

R

Q

D

G

G

E

W

W

M

L

H

A

T

T

G

G

D
|
D

L

K

A

Y

T

F

I

V

D

D

A

A

E

D

G

G

Y

Y

C

Y

N

W

I
x
H

V

A

G

G

R
|
R

V

S

K

D

D

D

M

M

L

L

I
x
K

R

V

G

G

E

I

N
x
W

V

V

Y

S

P

P

R

V

E

E

I

V

E

E

E

S

F

T

L

L

F

I

R

Q

H

H

P

P

K

A

V

V

Q

Q

A

E

V

C

Q

A

V

V

F

I

G

G

V

C

P

P

D

D

-

L

-

I

-

K

P

P

K

K

Y

-

G

-

E

-

V

-

C

-

A

A

W

F

I

I

V

I

L

L

K

K

P

P

G

-

D

Q

T

I

A

P

T

S

E

E

D

A

D

L

I

I

R

R

A

Q

F

I

-

T

-

D

-

H

C

C

R

T

D

E

Q

K

I

M

A

A

H

A

Y

Y

K

K

I

R

P

P

R

R

Y

W

I

I

R

E

F

F

V

V

D

T

E

E

M

L

P

P

M

K

T

T

I

A

T

T

G

G

K
|
K

V

I

Q

Q

K

R

F

F

V

K

M

L

R

R

E

S

R

A

M

A

T

K

L

L

E

A

L

A

G

A

L

L

active site: S175 (≠ T222), T320 (= T366), E321 (= E367), K418 (≠ I467), W423 (≠ N472), K502 (= K552)
binding 5'-O-[(S)-[(2-aminobenzoyl)oxy](hydroxy)phosphoryl]adenosine: F220 (≠ H266), T221 (≠ C267), F222 (= F268), A293 (= A338), S294 (≠ G339), E295 (≠ S340), A296 (≠ P341), G316 (≠ A362), I317 (≠ Y363), G318 (= G364), C319 (≠ M365), T320 (= T366), D397 (= D446), H409 (≠ I458), R412 (= R461), K502 (= K552)

6qjzA Identificationand characterization of an oxalylfrom grass pea (lathyrus sativuscoa-synthetase l.) (see paper)
27% identity, 90% coverage: 32:546/573 of query aligns to 5:493/504 of 6qjzA

query
sites
6qjzA

T

T

V

L

P

T

A

G

L

L

A

Q

E

S

T

V

V

A

A

K

R

T

F

F

P

P

D

S

R

R

E

R

A

G

V

I

V

S

F

L

R

A

E

G

Q

K

G

-

I

F

R

D

W

L

S

T

W

H

R

S

E

H

F

L

A

N

Q

E

V

L

V

V

D

E

A

S

M

A

A

A

G

D

G

H

L

L

R

I

L

S

L

A

G

G

L

I

E

K

R

P

G

N

D

D

R

V

I

V

G

A

I

L

W

T

S

F

P

P

N

N

R

T

V

V

E

E

W

Y

L

V

V

I

T

L

Q

F

F

L

A

A

T

V

A

I

R

R

L

V

G

R

L

A

V

T

L

A

V

A

N

P

I

L

N

N

P

A

A

A

Y

Y

R

T

L

A

A

E

E

E

L

F

E

E

Y

F

A

Y

L

L

N

S

K

D

V

S

G

E

C

S

K

K

A

L

I

L

V

-

A

-

E

-

S

-

F

-

K

-

T

-

S

-

R

-

Y

-

L

-

E

-

M

-

L

-

Q

-

T

-

L

L

A

T

P

P

E

-

L

L

K

E

S

G

C

N

E

K

P

P

G

A

T

Q

L

D

Q

A

A

A

A

S

R

K

L

L

P

S

A

-

L

-

R

-

W

-

V

-

I

I

R

P

M

L

G

G

A

S

E

A

R

S

T

L

P

T

G

K

M

S

L

E

N

E

Y

E

D

T

A

K

L

L

L

T

A

I

H

S

G

L

T

K

H

H

A

P

L

E

T

S

S

G

E

S

L

V

D

A

A

K

L

L

T

I

A

N

Q

E

L

P

D

S

R

-

Y

-

D

D

P

V

I

A

N

L

I

F

Q

L

F

H

T
|
T

S

S

G

G

T

T

G

S

A

R

P

P

K

K

G

G

A

V

T

P

L

L

T

T

H

Q

R

H

N

N

I

L

V

V

N

S

N
x
S

A

V

R

K

F

N

I

I

A

Q

M

S

A

V

M

Y

R

Q

F

L

S

T

E

E

Q

S

D

D

K

S

L

T

C

V

I

I

P

V

V

L

P

P

F

L

Y

F

H
|
H

C

V

F

H

G

G

M

L

V

I

L

A

A

G

V

L

L

L

A

S

C

S

V

L

S

G

T

S

G

G

A

A

C

A

M

V

V

V

F

L

P

P

G

A

E

A

A

G

F

R

E

F

P

S

A

A

A

S

T

T

M

F

-

W

A

K

A

D

V

M

S

I

E

Q

E

Y

R

N

C

A

T

T

A

W

L

Y

H

T

G

A

V

V

P

P

T

T

M

I

F

H

I

Q

A

I

Q

I

L

L

D

D

-

R

-

H

-

L

-

N

-

P

H

E

P

P

D

A

F

Y

A

P

K

K

Y

L

D

R

F

F

S

-

T

-

L

I

R

R

T

S

G

C

I
x
S

M
x
A

A
x
S

G

L

S

A

P

P

C

V

P

I

I

L

E

G

T

R

M

L

K

E

R

E

V

S

I

F

S

G

Q

-

M

-

H

-

M

-

A

A

Q

P

V

V

T

L

I

E

A

A

Y

Y

G
x
A

M
|
M

T
|
T

E
|
E

T

A

S

S

P

-

V

-

S

H

F

L

Q

M

S

S

T

N

T

P

D

L

P

P

L

Q

D

N

-

G

-

P

K

H

R

K

V

A

T

G

T

S

V

V

G

G

Q

K

I

P

Q

V

P

G

H

Q

L

-

E

E

V

M

K

A

L

I

V

L

D

D

G

E

T

S

G

G

E

R

I

V

V

L

P

E

V

A

G

G

E

V

K

N

G

G

E

E

L

V

C

C

T

I

R

R

G

G

Y

E

S

N

V

V

M

T

Q

K

G

G

Y

Y

W

K

D

N

E

E

P

A

R

A

T

N

R

T

E

A

A

A

I

F

R

L

D

F

G

G

W

W

M

F

H

H

T

T

G

G

D
|
D

L

I

A

G

T

Y

I

F

D

D

A

S

E

D

G

G

Y

Y

C

L

N

H

I
x
L

V

V

G

G

R

R

V

I

K
|
K

D

E

M

L

L

I

I
x
N

R

R

G

G

E

E

N
x
K

V

I

Y

S

P

P

R

I

E

E

I

V

E

D

E

A

F

V

L

L

F

L

R

S

H

H

P

P

K

D

V

V

Q

A

A

Q

V

A

Q

V

V

A

F

F

G

G

V

I

P

P

D

D

P

Q

K

K

Y

Y

G

G

E

E

V

I

C

H

A

C

W

A

I

I

V

I

L

P

K

R

P

E

G

G

D

S

T

N

A

I

T

D

E

A

D

E

I

V

R

L

A

T

F

F

C

C

R

K

D

K

Q

N

I

L

A

A

H

S

Y

F

K

K

I

V

P

P

R

K

Y

K

I

V

R

F

F

I

V

T

D

D

E

S

M

L

P

P

active site: T169 (= T222), S189 (≠ N242), H213 (= H266), T314 (= T366), E315 (= E367), N414 (≠ I467), K419 (≠ N472)
binding adenosine monophosphate: H213 (= H266), S288 (≠ I336), A289 (≠ M337), S290 (≠ A338), A312 (≠ G364), M313 (= M365), T314 (= T366), D393 (= D446), L405 (≠ I458), K410 (= K463), K419 (≠ N472)

Q9SMT7 Oxalate--CoA ligase; 4-coumarate--CoA ligase isoform 8; At4CL8; 4-coumarate--CoA ligase-like 10; Acyl-activating enzyme 3; Adenosine monophosphate binding protein 3; AtMPBP3; Oxalyl-CoA synthetase; EC 6.2.1.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
27% identity, 93% coverage: 32:562/573 of query aligns to 5:510/514 of Q9SMT7

query
sites
Q9SMT7

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TSGTT 170:174 (= TSGTT 222:226) binding ATP
H214 (= H266) binding ATP; mutation to A: Abolished activity.
S289 (≠ I336) binding oxalate; mutation to A: Abolished activity.
SAS 289:291 (≠ IMA 336:338) binding ATP
EA 310:311 (≠ IA 361:362) binding ATP
M314 (= M365) binding oxalate
T315 (= T366) binding ATP
H319 (≠ P370) binding oxalate; mutation to A: Abolished activity.
D394 (= D446) binding ATP
R409 (= R461) binding ATP; mutation to A: Abolished activity.
K500 (= K552) binding ATP; binding oxalate; mutation to A: Abolished activity.

5ie3A Crystal structure of a plant enzyme (see paper)
27% identity, 93% coverage: 32:562/573 of query aligns to 5:503/504 of 5ie3A

query
sites
5ie3A

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