SitesBLAST
Comparing RR42_RS27960 FitnessBrowser__Cup4G11:RR42_RS27960 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6t3eB Structure of thermococcus litoralis delta(1)-pyrroline-2-carboxylate reductase in complex with nadh and l-proline (see paper)
34% identity, 93% coverage: 1:308/330 of query aligns to 1:312/325 of 6t3eB
- binding 1,4-dihydronicotinamide adenine dinucleotide: S82 (≠ G80), T111 (= T109), G136 (= G134), V137 (≠ R135), Q138 (≠ V136), D159 (= D157), I160 (= I158), A199 (= A196), T200 (= T197), T201 (≠ L198), A202 (≠ S199), V206 (≠ L203), V221 (≠ I218), G222 (= G219), W223 (≠ G220), S296 (≠ A292), V297 (= V293), G298 (= G294)
- binding proline: R39 (= R35), M54 (= M53), K67 (= K65), R110 (= R108)
Q9FLY0 Protein SAR DEFICIENT 4; Ornithine cyclodeaminase-like protein; AtOCD from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 76% coverage: 61:310/330 of query aligns to 67:321/325 of Q9FLY0
- G89 (≠ S83) mutation to E: In sard4-3; compromises systemic acquired resistance (SAR).
- G138 (= G132) mutation to D: In sard4-4; compromises systemic acquired resistance (SAR).
- S205 (≠ T194) mutation to N: In sard4-1; compromises systemic acquired resistance (SAR).
2i99A Crystal structure of human mu_crystallin at 2.6 angstrom (see paper)
31% identity, 80% coverage: 48:310/330 of query aligns to 56:309/312 of 2i99A
- active site: G59 (≠ L51), S228 (≠ F221)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: D81 (≠ G72), S90 (≠ G80), H91 (≠ L81), R118 (= R108), T119 (= T109), G142 (= G132), A143 (= A133), G144 (= G134), V145 (≠ R135), Q146 (≠ V136), N167 (≠ D157), R168 (≠ I158), T169 (≠ N159), V203 (≠ A196), T204 (= T197), L205 (= L198), A206 (≠ S199), V225 (≠ I218), G226 (= G219), S291 (≠ A292), L292 (≠ V293), G293 (= G294)
Q14894 Ketimine reductase mu-crystallin; NADP-regulated thyroid-hormone-binding protein; EC 1.5.1.25 from Homo sapiens (Human) (see paper)
31% identity, 80% coverage: 48:310/330 of query aligns to 57:310/314 of Q14894
O28608 Alanine dehydrogenase; AlaDH; EC 1.4.1.1 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
29% identity, 93% coverage: 2:308/330 of query aligns to 4:306/322 of O28608
1omoA Alanine dehydrogenase dimer w/bound NAD (archaeal) (see paper)
29% identity, 93% coverage: 2:308/330 of query aligns to 4:306/320 of 1omoA
- active site: R52 (≠ L51), D219 (≠ F221)
- binding nicotinamide-adenine-dinucleotide: T109 (= T109), G134 (= G134), T135 (≠ R135), Q136 (≠ V136), Y156 (≠ W156), D157 (= D157), V158 (≠ I158), R159 (≠ N159), T195 (= T197), P196 (≠ L198), G217 (= G219), D219 (≠ F221), K223 (≠ M225), S290 (≠ A292), T291 (≠ V293), G292 (= G294)
4bv9A Crystal structure of the NADPH form of mouse mu-crystallin. (see paper)
31% identity, 80% coverage: 48:310/330 of query aligns to 55:301/303 of 4bv9A
- active site: G58 (≠ L51), S220 (≠ F221)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S82 (≠ G80), H83 (≠ L81), T111 (= T109), G134 (= G132), G136 (= G134), V137 (≠ R135), Q138 (≠ V136), N159 (≠ D157), R160 (≠ I158), T161 (≠ N159), V195 (≠ A196), T196 (= T197), M197 (≠ L198), A198 (≠ S199), V217 (≠ I218), G218 (= G219), S283 (≠ A292), L284 (≠ V293), G285 (= G294)
- binding pyruvic acid: M60 (= M53), K73 (= K65), R110 (= R108)
Sites not aligning to the query:
4bvaA Crystal structure of the NADPH-t3 form of mouse mu-crystallin. (see paper)
31% identity, 80% coverage: 48:310/330 of query aligns to 56:300/303 of 4bvaA
- active site: G59 (≠ L51), S219 (≠ F221)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T106 (= T105), R109 (= R108), T110 (= T109), G135 (= G134), V136 (≠ R135), Q137 (≠ V136), N158 (≠ D157), R159 (≠ I158), T160 (≠ N159), N163 (≠ Q162), V194 (≠ A196), T195 (= T197), M196 (≠ L198), A197 (≠ S199), V216 (≠ I218), S282 (≠ A292), L283 (≠ V293), G284 (= G294)
- binding 3,5,3'triiodothyronine: F57 (≠ T49), G59 (≠ L51), V76 (= V67), F78 (≠ I69), S219 (≠ F221), R220 (≠ T222), W223 (≠ M225), E247 (vs. gap)
Sites not aligning to the query:
6rqaB Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
31% identity, 75% coverage: 62:308/330 of query aligns to 66:309/322 of 6rqaB
- binding Tb-Xo4: N76 (≠ G72)
- binding nicotinamide-adenine-dinucleotide: T113 (= T109), G138 (= G134), Q140 (≠ V136), P162 (≠ I158), H163 (≠ N159), I199 (≠ A196), T200 (= T197), S201 (≠ L198), S202 (= S199), M221 (≠ I218), G222 (= G219), D224 (≠ F221), K228 (≠ M225), G293 (≠ A292), T294 (≠ V293), G295 (= G294)
Sites not aligning to the query:
6rqaA Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
31% identity, 75% coverage: 62:308/330 of query aligns to 66:309/322 of 6rqaA
- binding nicotinamide-adenine-dinucleotide: H85 (≠ L81), T113 (= T109), G138 (= G134), H139 (≠ R135), Q140 (≠ V136), N161 (≠ D157), P162 (≠ I158), H163 (≠ N159), M166 (≠ Q162), I199 (≠ A196), T200 (= T197), S201 (≠ L198), S202 (= S199), M221 (≠ I218), G222 (= G219), D224 (≠ F221), K228 (≠ M225), G293 (≠ A292)
A1B8Z0 Iminosuccinate reductase; EC 1.4.1.- from Paracoccus denitrificans (strain Pd 1222) (see paper)
31% identity, 75% coverage: 62:308/330 of query aligns to 64:307/320 of A1B8Z0
Q88H32 Ornithine cyclodeaminase; OCD; EC 4.3.1.12 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
28% identity, 87% coverage: 14:299/330 of query aligns to 21:300/350 of Q88H32
- R45 (≠ V39) binding
- K69 (= K65) binding
- T84 (≠ G80) binding
- R112 (= R108) binding ; binding
- AQ 139:140 (≠ RV 135:136) binding
- D161 (= D157) binding
- T202 (= T197) binding
- VGGD 225:228 (≠ IGGF 218:221) binding
- D228 (≠ F221) binding
- K232 (≠ M225) binding
- S293 (≠ A292) binding
- V294 (= V293) binding
Sites not aligning to the query:
1u7hA Structure and a proposed mechanism for ornithine cyclodeaminase from pseudomonas putida (see paper)
28% identity, 87% coverage: 14:299/330 of query aligns to 20:299/341 of 1u7hA
- active site: E55 (≠ L51), D227 (≠ F221)
- binding nicotinamide-adenine-dinucleotide: T83 (≠ G80), R111 (= R108), T112 (= T109), G137 (= G134), A138 (≠ R135), Q139 (≠ V136), D160 (= D157), T161 (≠ I158), V200 (≠ A196), T201 (= T197), A202 (≠ L198), I209 (≠ L203), V224 (≠ I218), G225 (= G219), D227 (≠ F221), K231 (≠ M225), S292 (≠ A292), V293 (= V293), G294 (= G294)
1x7dA Crystal structure analysis of ornithine cyclodeaminase complexed with NAD and ornithine to 1.6 angstroms (see paper)
28% identity, 87% coverage: 14:299/330 of query aligns to 20:299/340 of 1x7dA
- active site: E55 (≠ L51), D227 (≠ F221)
- binding nicotinamide-adenine-dinucleotide: T83 (≠ G80), R111 (= R108), T112 (= T109), G137 (= G134), A138 (≠ R135), Q139 (≠ V136), D160 (= D157), T161 (≠ I158), V200 (≠ A196), T201 (= T197), A202 (≠ L198), I209 (≠ L203), V224 (≠ I218), G225 (= G219), D227 (≠ F221), K231 (≠ M225), S292 (≠ A292), V293 (= V293), G294 (= G294)
- binding L-ornithine: R44 (≠ V39), V53 (≠ T49), E55 (≠ L51), M57 (= M53), K68 (= K65), V70 (= V67), N71 (≠ T68), G72 (≠ I69), R111 (= R108), D227 (≠ F221), V293 (= V293)
5gziA Cyclodeaminase_pa
28% identity, 77% coverage: 47:299/330 of query aligns to 63:312/354 of 5gziA
- binding nicotinamide-adenine-dinucleotide: Y85 (≠ I69), T97 (≠ G80), R125 (= R108), T126 (= T109), G151 (= G134), A152 (≠ R135), Q153 (≠ V136), D174 (= D157), T175 (≠ I158), H179 (≠ Q162), A212 (= A196), T213 (= T197), S214 (vs. gap), V215 (vs. gap), V237 (≠ I218), G238 (= G219), A239 (≠ G220), S305 (≠ A292), T306 (≠ V293), G307 (= G294)
- binding (2S)-piperidine-2-carboxylic acid: K81 (= K65), R125 (= R108), A239 (≠ G220), T306 (≠ V293), G307 (= G294)
Sites not aligning to the query:
5gzlA Cyclodeaminase_pa
28% identity, 77% coverage: 47:299/330 of query aligns to 63:312/357 of 5gzlA
- binding lysine: I65 (≠ T49), E67 (≠ L51), D240 (≠ F221), R267 (≠ K248), E268 (≠ A249)
- binding nicotinamide-adenine-dinucleotide: Y85 (≠ I69), T97 (≠ G80), I98 (≠ L81), T126 (= T109), G151 (= G134), A152 (≠ R135), Q153 (≠ V136), D174 (= D157), T175 (≠ I158), H179 (≠ Q162), A212 (= A196), T213 (= T197), S214 (vs. gap), V222 (≠ L203), V237 (≠ I218), G238 (= G219), A239 (≠ G220), D240 (≠ F221), K244 (≠ M225), S305 (≠ A292), T306 (≠ V293), G307 (= G294)
Sites not aligning to the query:
5yu4A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
28% identity, 77% coverage: 47:299/330 of query aligns to 59:308/344 of 5yu4A
- binding 2,4-diaminobutyric acid: E63 (≠ L51), K77 (= K65), R121 (= R108), T302 (≠ V293), G303 (= G294)
- binding nicotinamide-adenine-dinucleotide: Y81 (≠ I69), T93 (≠ G80), I94 (≠ L81), R121 (= R108), T122 (= T109), G147 (= G134), A148 (≠ R135), Q149 (≠ V136), D170 (= D157), T171 (≠ I158), H175 (≠ Q162), A208 (= A196), T209 (= T197), S210 (vs. gap), V211 (vs. gap), V218 (≠ L203), V233 (≠ I218), A235 (≠ G220), S301 (≠ A292), T302 (≠ V293), G303 (= G294)
5yu3A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
28% identity, 77% coverage: 47:299/330 of query aligns to 59:308/344 of 5yu3A
- binding nicotinamide-adenine-dinucleotide: Y81 (≠ I69), T93 (≠ G80), I94 (≠ L81), T122 (= T109), G147 (= G134), A148 (≠ R135), Q149 (≠ V136), D170 (= D157), T171 (≠ I158), A208 (= A196), T209 (= T197), S210 (vs. gap), V211 (vs. gap), V233 (≠ I218), A235 (≠ G220), S301 (≠ A292), T302 (≠ V293), G303 (= G294)
- binding proline: M65 (= M53), K77 (= K65), R121 (= R108)
4mp6A Staphyloferrin b precursor biosynthetic enzyme sbnb bound to citrate and NAD+ (see paper)
24% identity, 68% coverage: 86:308/330 of query aligns to 98:320/334 of 4mp6A
- active site: M236 (≠ I218)
- binding nicotinamide-adenine-dinucleotide: R120 (= R108), T121 (= T109), G146 (= G134), L147 (≠ R135), I148 (≠ V136), D170 (= D157), Q171 (≠ I158), C211 (≠ A196), T212 (= T197), V213 (≠ L198), I233 (≠ L215), G306 (= G294)
Sites not aligning to the query:
4mpdA Staphyloferrin b precursor biosynthetic enzyme sbnb bound a- ketoglutarate and NAD+ (see paper)
25% identity, 68% coverage: 86:308/330 of query aligns to 86:304/318 of 4mpdA
Sites not aligning to the query:
Query Sequence
>RR42_RS27960 FitnessBrowser__Cup4G11:RR42_RS27960
MLILDSDTTRALLPFAKLVPALRDAFIRGCEVPQRHSHVIDTHGAAPGTMLLMPAWQADA
YLGVKTVTIYPGNTLSGLPGLYSTYLLHDACTGKPLSMIDGNEITSRRTAAASALAASYL
SPNYASSMLVLGAGRVASLLPQAYRAVRPIQRVAVWDINASQGEALAARLRADGFEARFV
ATIAAACEDADIVTSATLSTSPLVQRAWLRPGTHLDLIGGFTPAMREADDGCFESTAVFV
DTSEAVLKAGDLLHPIEAGVLKATDIRGTLADLCQQRHRGRTDDTEITVFKAVGTALEDL
AAAVMAYEGFEGLPRTTPRAESHGNVRRQA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory