SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS29875 FitnessBrowser__Cup4G11:RR42_RS29875 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q007T0 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Sus scrofa (Pig) (see paper)
29% identity, 23% coverage: 7:102/419 of query aligns to 168:270/280 of Q007T0

query
sites
Q007T0

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V

I

H

L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R

Y

N

L

G

G

P

P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L

H

T

T

C

I

R

M

N

N

C
|
C

E

T

S

G

T

T

C
|
C

P

P

S

K

G

G

V

L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

C186 (= C25) binding
C189 (= C28) binding
C192 (= C31) binding
C196 (= C35) binding
W201 (≠ L40) binding
C243 (= C75) binding
C249 (= C81) binding
C253 (= C85) binding

Sites not aligning to the query:

93 binding
98 binding
101 binding
113 binding

3sfeB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and thiabendazole (see paper)
29% identity, 23% coverage: 7:102/419 of query aligns to 133:235/240 of 3sfeB

query
sites
3sfeB

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

L

W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C161 (= C35), Y171 (≠ R45), P174 (= P48), C208 (= C75), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (≠ R49), C218 (= C85), P219 (= P86), K220 (≠ S87), L222 (≠ V89)
binding 2-(1,3-thiazol-4-yl)-1h-benzimidazole: H209 (≠ L76), I211 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 61, 63, 64, 65, 66, 78

8gs8B Cryo-em structure of the human respiratory complex ii (see paper)
29% identity, 23% coverage: 7:102/419 of query aligns to 134:236/239 of 8gs8B

query
sites
8gs8B

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V

I

H

L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P

P

S

K

G

G

V

L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C162 (= C35), Y172 (≠ R45), P175 (= P48), C209 (= C75), H210 (≠ L76), T211 (= T77), I212 (≠ C78), M213 (≠ R79), N214 (= N80), C215 (= C81)
binding iron/sulfur cluster: C152 (= C25), C155 (= C28), C158 (= C31), A176 (≠ R49), C219 (= C85)
binding ubiquinone-1: P163 (= P36), W167 (≠ L40), I212 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 59, 60, 64, 65, 66, 67, 79

3sfdB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and pentachlorophenol (see paper)
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3sfdB

query
sites
3sfdB

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L

H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V

L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding pentachlorophenol: P161 (= P36), W165 (≠ L40), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), K219 (≠ S87)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 64, 65, 77

3aegB Crystal structure of porcine heart mitochondrial complex ii bound with n-biphenyl-3-yl-2-iodo-benzamide
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3aegB

query
sites
3aegB

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N

N

C
|
C

E

T

S

G

T

T

C
|
C

P

P

S

K

G

G

V

L

E

N

Y

P

G

G

K

K

L

A

V
x
I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding N-biphenyl-3-yl-2-iodobenzamide: S162 (≠ T37), W164 (≠ L39), W165 (≠ L40), H208 (≠ L76)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), C213 (= C81), I227 (≠ V95)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 75, 77

3aeeB Crystal structure of porcine heart mitochondrial complex ii bound with atpenin a5
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3aeeB

query
sites
3aeeB

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V

I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding 3-[(2s,4s,5r)-5,6-dichloro-2,4-dimethyl-1-oxohexyl]-4-hydroxy-5,6-dimethoxy-2(1h)-pyridinone: W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3aedB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-phenyl-benzamide
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3aedB

query
sites
3aedB

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P

P

S

K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding 2-iodo-N-phenylbenzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3aecB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-(1-methylethyl)-benzamid
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3aecB

query
sites
3aecB

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding 2-iodo-N-(1-methylethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 75, 77

3aebB Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-phenoxy-phenyl)-2-trifluoromethyl-benzamide
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3aebB

query
sites
3aebB

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P

P

S

K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-(3-phenoxyphenyl)-2-(trifluoromethyl)benzamide: W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 77

3aeaB Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-dimethylaminomethyl-phenyl)-2-trifluoromethyl-benzamide (see paper)
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3aeaB

query
sites
3aeaB

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-{3-[(dimethylamino)methyl]phenyl}-2-(trifluoromethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3ae9B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-pentafluorophenyloxy-phenyl)-2-trifluoromethyl-benzamide (see paper)
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3ae9B

query
sites
3ae9B

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P

P

S

K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-[3-(pentafluorophenoxy)phenyl]-2-(trifluoromethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3ae8B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-isopropoxy-phenyl)-2-trifluoromethylbenzamide
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3ae8B

query
sites
3ae8B

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R

Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N

N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), C213 (= C81)
binding N-[3-(1-methylethoxy)phenyl]-2-(trifluoromethyl)benzamide: S162 (≠ T37), W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 75, 77

3ae7B Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-(3-isopropoxy-phenyl)-benzamide (see paper)
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3ae7B

query
sites
3ae7B

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N

N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V

L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding 2-iodo-N-[3-(1-methylethoxy)phenyl]benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 75, 77

3ae6B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-isopropoxy-phenyl)-phthalamicacid
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3ae6B

query
sites
3ae6B

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L

H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding 2-{[3-(1-methylethoxy)phenyl]carbamoyl}benzoic acid: S162 (≠ T37), W165 (≠ L40)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 56, 57, 62, 63, 65, 77

3ae4B Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-methyl-benzamide
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3ae4B

query
sites
3ae4B

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H

L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R

Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V

L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), S162 (≠ T37), P173 (= P48), C207 (= C75), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding 2-iodo-N-methylbenzamide: W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 75, 77

3ae3B Crystal structure of porcine heart mitochondrial complex ii bound with 2-nitro-n-phenyl-benzamide
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3ae3B

query
sites
3ae3B

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding 2-nitro-N-phenylbenzamide: P161 (= P36), S162 (≠ T37), W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3ae1B Crystal structure of porcine heart mitochondrial complex ii bound with n-phenyl-2-(trifluoromethyl)-benzamide
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3ae1B

query
sites
3ae1B

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

L

W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P

P

S
x
K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), K219 (≠ S87), L221 (≠ V89)
binding N-phenyl-2-(trifluoromethyl)benzamide: H208 (≠ L76), I210 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3abvB Crystal structure of porcine heart mitochondrial complex ii bound with n-biphenyl-3-yl-2-trifluoromethyl-benzamide (see paper)
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 3abvB

query
sites
3abvB

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R

Y

N

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N

N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), C213 (= C81)
binding N-biphenyl-3-yl-2-(trifluoromethyl)benzamide: W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

1zp0B Crystal structure of mitochondrial respiratory complex ii bound with 3-nitropropionate and 2-thenoyltrifluoroacetone (see paper)
29% identity, 23% coverage: 7:102/419 of query aligns to 132:234/239 of 1zp0B

query
sites
1zp0B

H

Q

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

E

A

R

E

E

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

N

L

G

G

P
|
P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

F

-

T

E

E

V

R

T

L

A

A

S

K

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N

N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V

L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), C217 (= C85), P218 (= P86)
binding 4,4,4-trifluoro-1-thien-2-ylbutane-1,3-dione: P161 (= P36), W165 (≠ L40), H208 (≠ L76)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

Q9YHT2 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Gallus gallus (Chicken) (see 2 papers)
28% identity, 23% coverage: 7:102/419 of query aligns to 178:280/290 of Q9YHT2

query
sites
Q9YHT2

H

E

E

Q

F

Y

A

L

N

Q

T

S

P

I

E

E

G

D

A

R

E

Q

A

K

E

L

E

D

I

G

L

L

R

Y

K

E

C
|
C

V

I

H

L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

L

W

L
x
W

L

N

G

G

D

D

E

K

R

Y

N

L

G

G

P

P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

L

M

L

I

E

D

G

S

-

R

-

D

-

Y

-

T

E

E

V

R

T

L

A

A

S

Q

T

L

Q

Q

Q

D

-

P

-

F

N

S

L

L

D

Y

R

R

C
|
C

L

H

T

T

C

I

R

M

N

N

C
|
C

E

T

S

R

T

T

C
|
C

P

P

S

K

G

G

V

L

E

N

Y

P

G

G

K

K

L

A

V

I

H

A

I

E

G

I

R

K

K

K

L

M

V

M

C196 (= C25) binding
C199 (= C28) binding
C202 (= C31) binding
C206 (= C35) binding
W211 (≠ L40) binding
C253 (= C75) binding
C259 (= C81) binding
C263 (= C85) binding

Sites not aligning to the query:

103 binding
108 binding
111 binding
123 binding

Query Sequence

>RR42_RS29875 FitnessBrowser__Cup4G11:RR42_RS29875
MQTKLSHEFANTPEGAEAEEILRKCVHCGFCTATCPTYLLLGDERNGPRGRIYLMKQLLE
GEEVTASTQQNLDRCLTCRNCESTCPSGVEYGKLVHIGRKLVDERIPRPPKDRAVRWLLR
EGLTSPLFLPAMKIGRMVRPLLPSKLANKLTPSRPAGQRPTRRHARKVLMLEGCVQPAMM
PNINSATARVLDAADFQVVSVPRAGCCGAIRLHTGDHKGGLDDMRRNIDAWWPAISSGEV
EAIVMNASGCGATVKDYADLLKHDAEYAEKAARVSELTRDLSELLPELLPRLRGKVKACD
KPALAFHPPCTLQHGQKLRGVVEEHLGQLGFSVHRASVESHLCCGSAGTYSVLQPEISLQ
LRDRKIEHLSEKQPDCIVSANIGCIQHLQSGTPTPVKHWIEVLDDAIEPAVQPEEQVFI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory