SitesBLAST
Comparing RR42_RS31735 FitnessBrowser__Cup4G11:RR42_RS31735 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3c4jA Abc protein artp in complex with atp-gamma-s
64% identity, 97% coverage: 6:250/253 of query aligns to 3:242/242 of 3c4jA
3c41J Abc protein artp in complex with amp-pnp/mg2+
64% identity, 97% coverage: 6:250/253 of query aligns to 3:242/242 of 3c41J
2olkA Abc protein artp in complex with adp-beta-s
64% identity, 97% coverage: 6:250/253 of query aligns to 3:242/242 of 2olkA
2oljA Abc protein artp in complex with adp/mg2+
64% identity, 97% coverage: 6:250/253 of query aligns to 3:242/242 of 2oljA
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
62% identity, 97% coverage: 6:250/253 of query aligns to 2:240/241 of 4u00A
4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
56% identity, 97% coverage: 6:250/253 of query aligns to 1:240/240 of 4ymuJ
- binding adenosine-5'-triphosphate: F11 (= F16), V16 (= V21), S36 (= S41), G37 (= G42), S38 (= S43), G39 (= G44), K40 (= K45), S41 (= S46), T42 (= T47), E162 (= E172), H194 (= H204)
- binding magnesium ion: S41 (= S46), E162 (= E172)
1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
50% identity, 97% coverage: 7:252/253 of query aligns to 3:255/258 of 1b0uA
P02915 Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP; EC 7.4.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
50% identity, 99% coverage: 1:250/253 of query aligns to 2:257/258 of P02915
- S41 (= S41) binding ATP
- G42 (= G42) binding ATP
- G44 (= G44) binding ATP
- K45 (= K45) binding ATP
- S46 (= S46) binding ATP
- T47 (= T47) binding ATP
P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 97% coverage: 6:250/253 of query aligns to 1:245/343 of P30750
- 40:46 (vs. 41:47, 86% identical) binding ATP
- E166 (= E172) mutation to Q: Exhibits little ATPase activity.
Sites not aligning to the query:
- 278:283 binding L-methionine
- 295 N→A: Reduces the binding of L-methionine to undetectable levels.
- 295:296 binding L-methionine
3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
39% identity, 97% coverage: 6:250/253 of query aligns to 2:246/344 of 3tuzC
Sites not aligning to the query:
3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
39% identity, 97% coverage: 6:250/253 of query aligns to 2:246/344 of 3tuiC
6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
39% identity, 97% coverage: 6:250/253 of query aligns to 2:246/344 of 6cvlD
- binding phosphothiophosphoric acid-adenylate ester: F12 (= F16), Q14 (vs. gap), I19 (≠ V21), S41 (= S41), G42 (= G42), A43 (≠ S43), G44 (= G44), K45 (= K45), S46 (= S46), T47 (= T47), N141 (≠ S146), S143 (= S148), Q146 (= Q151), H200 (= H204)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 96% coverage: 5:246/253 of query aligns to 2:234/369 of P19566
- L86 (= L98) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P173) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D178) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
38% identity, 95% coverage: 7:246/253 of query aligns to 11:252/257 of P0AAH0
- G48 (= G44) mutation to I: Loss of phosphate transport.
- K49 (= K45) mutation to Q: Loss of phosphate transport.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
38% identity, 96% coverage: 5:246/253 of query aligns to 2:234/371 of P68187
- A85 (≠ N97) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ S119) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ A127) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L130) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (= E132) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (= A137) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G150) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D171) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ E240) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
38% identity, 96% coverage: 5:246/253 of query aligns to 1:233/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
38% identity, 96% coverage: 5:246/253 of query aligns to 1:233/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F16), S37 (= S41), G38 (= G42), C39 (≠ S43), G40 (= G44), K41 (= K45), S42 (= S46), T43 (= T47), Q81 (= Q94), R128 (≠ A142), A132 (≠ S146), S134 (= S148), G136 (= G150), Q137 (= Q151), E158 (= E172), H191 (= H204)
- binding magnesium ion: S42 (= S46), Q81 (= Q94)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
38% identity, 96% coverage: 5:246/253 of query aligns to 1:233/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F16), G38 (= G42), C39 (≠ S43), G40 (= G44), K41 (= K45), S42 (= S46), T43 (= T47), R128 (≠ A142), S134 (= S148), Q137 (= Q151)
- binding beryllium trifluoride ion: S37 (= S41), G38 (= G42), K41 (= K45), Q81 (= Q94), S134 (= S148), G136 (= G150), H191 (= H204)
- binding magnesium ion: S42 (= S46), Q81 (= Q94)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
38% identity, 96% coverage: 5:246/253 of query aligns to 1:233/371 of 3puwA