SitesBLAST
Comparing RR42_RS33330 FitnessBrowser__Cup4G11:RR42_RS33330 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
48% identity, 93% coverage: 15:303/312 of query aligns to 14:302/311 of 3bazA
- active site: L98 (= L99), R230 (= R231), A251 (= A252), D254 (= D255), E259 (= E260), H277 (= H278)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (= V75), G149 (= G150), L150 (≠ M151), G151 (= G152), R152 (= R153), I153 (= I154), S172 (= S173), R173 (= R174), S174 (≠ R175), C201 (≠ T202), P202 (≠ A203), T207 (= T208), I228 (= I229), G229 (≠ A230), R230 (= R231), D254 (= D255), H277 (= H278), G279 (≠ A280)
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
48% identity, 93% coverage: 15:303/312 of query aligns to 16:304/313 of Q65CJ7
5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
42% identity, 97% coverage: 2:305/312 of query aligns to 2:303/319 of 5v7nA
- active site: L95 (= L99), R229 (= R231), D253 (= D255), E258 (= E260), H276 (= H278)
- binding 2-keto-D-gluconic acid: G70 (= G74), V71 (= V75), G72 (= G76), R229 (= R231), H276 (= H278), S279 (= S281), R285 (= R287)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (= V75), V99 (= V103), L149 (≠ M151), G150 (= G152), R151 (= R153), I152 (= I154), T171 (≠ S173), R172 (= R174), V200 (≠ T202), P201 (≠ A203), S205 (≠ E207), T206 (= T208), V227 (≠ I229), G228 (≠ A230), R229 (= R231), H276 (= H278), A278 (= A280)
5v6qB Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and malonate (see paper)
42% identity, 97% coverage: 2:305/312 of query aligns to 3:304/319 of 5v6qB
- active site: L96 (= L99), R230 (= R231), D254 (= D255), E259 (= E260), H277 (= H278)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (= V75), V100 (= V103), F148 (≠ V149), L150 (≠ M151), G151 (= G152), R152 (= R153), I153 (= I154), T172 (≠ S173), R173 (= R174), V201 (≠ T202), P202 (≠ A203), S206 (≠ E207), T207 (= T208), V228 (≠ I229), G229 (≠ A230), R230 (= R231), H277 (= H278), A279 (= A280)
5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
42% identity, 97% coverage: 2:305/312 of query aligns to 1:302/317 of 5v7gA
- active site: L94 (= L99), R228 (= R231), D252 (= D255), E257 (= E260), H275 (= H278)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V70 (= V75), V98 (= V103), F146 (≠ V149), L148 (≠ M151), G149 (= G152), R150 (= R153), I151 (= I154), T170 (≠ S173), R171 (= R174), V199 (≠ T202), P200 (≠ A203), S204 (≠ E207), T205 (= T208), V226 (≠ I229), G227 (≠ A230), R228 (= R231), H275 (= H278), A277 (= A280)
- binding oxalate ion: G69 (= G74), V70 (= V75), G71 (= G76), R228 (= R231), H275 (= H278)
5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
42% identity, 97% coverage: 2:305/312 of query aligns to 1:302/318 of 5j23A
- active site: L94 (= L99), R228 (= R231), D252 (= D255), E257 (= E260), H275 (= H278)
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (= V75), L148 (≠ M151), G149 (= G152), R150 (= R153), I151 (= I154), T170 (≠ S173), R171 (= R174), P200 (≠ A203), S204 (≠ E207), T205 (= T208), R228 (= R231)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
44% identity, 86% coverage: 34:301/312 of query aligns to 35:311/334 of 5aovA
- active site: L100 (= L99), R241 (= R231), D265 (= D255), E270 (= E260), H288 (= H278)
- binding glyoxylic acid: M52 (≠ R51), L53 (≠ A52), L53 (≠ A52), Y74 (≠ F73), A75 (≠ G74), V76 (= V75), G77 (= G76), R241 (= R231), H288 (= H278)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V75), T104 (≠ V103), F158 (≠ M151), G159 (= G152), R160 (= R153), I161 (= I154), S180 (= S173), R181 (= R174), A211 (= A201), V212 (≠ T202), P213 (≠ A203), T218 (= T208), I239 (= I229), A240 (= A230), R241 (= R231), H288 (= H278), G290 (≠ A280)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
44% identity, 86% coverage: 34:301/312 of query aligns to 34:310/332 of 6biiA
- active site: L99 (= L99), R240 (= R231), D264 (= D255), E269 (= E260), H287 (= H278)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V75), T103 (≠ V103), G156 (= G150), F157 (≠ M151), G158 (= G152), R159 (= R153), I160 (= I154), A179 (vs. gap), R180 (≠ H172), S181 (= S173), K183 (≠ R175), V211 (≠ T202), P212 (≠ A203), E216 (= E207), T217 (= T208), V238 (≠ I229), A239 (= A230), R240 (= R231), D264 (= D255), H287 (= H278), G289 (≠ A280)
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
42% identity, 96% coverage: 1:301/312 of query aligns to 1:311/333 of 2dbqA
- active site: L100 (= L99), R241 (= R231), D265 (= D255), E270 (= E260), H288 (= H278)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V75), T104 (≠ V103), L158 (≠ M151), G159 (= G152), R160 (= R153), I161 (= I154), S180 (= S173), R181 (= R174), T182 (≠ R175), A211 (= A201), V212 (≠ T202), P213 (≠ A203), T218 (= T208), I239 (= I229), A240 (= A230), R241 (= R231), D265 (= D255), H288 (= H278), G290 (≠ A280)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
42% identity, 96% coverage: 1:301/312 of query aligns to 1:311/334 of O58320
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
42% identity, 76% coverage: 62:298/312 of query aligns to 66:309/324 of 2gcgA
- active site: L103 (= L99), R241 (= R231), D265 (= D255), E270 (= E260), H289 (= H278)
- binding (2r)-2,3-dihydroxypropanoic acid: S78 (≠ G74), V79 (= V75), G80 (= G76), R241 (= R231), H289 (= H278)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (= V75), T107 (≠ V103), G156 (= G150), G158 (= G152), I160 (= I154), G180 (≠ S173), R181 (= R174), R184 (= R175), C212 (≠ T202), S213 (≠ A203), T218 (= T208), I239 (= I229), R241 (= R231), D265 (= D255), H289 (= H278), G291 (≠ A280)
Sites not aligning to the query:
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
42% identity, 76% coverage: 62:298/312 of query aligns to 70:313/328 of Q9UBQ7
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
38% identity, 83% coverage: 44:303/312 of query aligns to 42:303/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
38% identity, 83% coverage: 44:303/312 of query aligns to 41:302/526 of 3dc2A
Sites not aligning to the query:
4e5mA Thermostable phosphite dehydrogenase e175a/a176r in complex with NADP (see paper)
35% identity, 78% coverage: 56:298/312 of query aligns to 57:312/329 of 4e5mA
- active site: L100 (= L99), R237 (= R231), D261 (= D255), E266 (= E260), H292 (= H278)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K76 (≠ V75), L100 (= L99), T104 (≠ V103), G154 (= G152), A155 (≠ R153), I156 (= I154), R176 (= R174), L208 (≠ T202), P209 (≠ A203), T214 (= T208), P235 (≠ I229), C236 (≠ A230), R237 (= R231), H292 (= H278), G294 (≠ A280)
4e5pA Thermostable phosphite dehydrogenase a176r variant in complex with nad (see paper)
35% identity, 78% coverage: 56:298/312 of query aligns to 57:312/332 of 4e5pA
- active site: L100 (= L99), R237 (= R231), D261 (= D255), E266 (= E260), H292 (= H278)
- binding nicotinamide-adenine-dinucleotide: K76 (≠ V75), L100 (= L99), T104 (≠ V103), G154 (= G152), A155 (≠ R153), I156 (= I154), A175 (≠ S173), R176 (= R174), L208 (≠ T202), P209 (≠ A203), T214 (= T208), P235 (≠ I229), C236 (≠ A230), R237 (= R231), H292 (= H278)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
34% identity, 77% coverage: 48:288/312 of query aligns to 51:293/533 of O43175
- T78 (≠ V75) binding
- R135 (≠ K132) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (= RI 153:154) binding
- D175 (≠ S173) binding
- T207 (= T202) binding
- CAR 234:236 (≠ IAR 229:231) binding
- D260 (= D255) binding
- V261 (= V256) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIAS 278:281) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
34% identity, 79% coverage: 44:288/312 of query aligns to 42:288/301 of 6rj5A
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
34% identity, 79% coverage: 44:288/312 of query aligns to 41:287/297 of 6rj3A
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
34% identity, 79% coverage: 44:288/312 of query aligns to 42:288/303 of 6plgA
Query Sequence
>RR42_RS33330 FitnessBrowser__Cup4G11:RR42_RS33330
MKPRLAQHGRLPAALEASLAEHYDVHPLWAETDPAAFLASHGGEFAALTTRAAIGVDAAM
IAAMPNLKVISSFGVGLDKIDLDAARARGIAVGYTPDVLNDCVADTAFGLLMDVARGFSA
ADRFVRRGEWPKAQFPLATRVSGKRMGIVGMGRIGRVIARRGVGFDMEVRYHSRRPAQDA
PYRHEPSLSELARWADFLVIATAGGPETRHLVSAEVLEALGPNGFLINIARGTVVDETAL
VDALVQGRIAGAGLDVFENEPQVPQALFALDNVVLLPHIASATHETRNAMADLVFENLQS
FFASGAVKASAV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory