SitesBLAST

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
37% identity, 73% coverage: 21:209/258 of query aligns to 39:236/382 of 7ahhC

query
sites
7ahhC

T
|
T

L
x
V

A
x
G

L

V

S

Y

Q

D

V

T

N

N

L

F

T

E

M

I

E

N

R

E

G

G

D

E

F

I

V

F

V

V

A

I

L

M

G

G

A

L

S

S

G
|
G

C

S

G
|
G

K
|
K

T

S

T

T

L

L

S

R

C

L

I

L

A

N

G

R

F

L

M

I

Q

E

P

P

S

T

E

S

G

G

E

K

I

I

R

F

L

I

D

D

G

N

K

Q

S

D

V

V

H

A

G

T

P

L

G

N

A

K

E

E

R

D

-

L

-

Q

-

V

-

R

-

K

-

T

-

M

G

S

V

M

V

V

F

F

Q

Q

K

N

H

F

A

G

L

L

M

F

P

P

W

H

L

R

N

T

V

I

L

L

D

E

N

N

V

T

A

E

L

Y

G

G

L

L

R

E

L

V

R

Q

G

N

V

V

P

P

R

K

A

E

E

E

R

R

L

R

R

K

I

R

A

A

Q

E

E

K

K

A

L

L

A

D

L

N

V

A

G

N

L

L

E

L

K

D

V

F

A

K

A

D

K

Q

P

Y

V

P

Y

K

Q

Q

L

L

S

S

G

G

M

M

Q

Q

R

R

V

V

G

G

I

L

A

A

R

R

T

A

L

L

A

A

N

N

D

D

P

P

A

E

V

I

M

L

L

L

M

M

D
|
D

E
|
E

P

A

L

F

G

S

A

A

L

L

D

D

A

P

L

L

T

I

R

R

E

R

T

E

I

M

Q

Q

A

D

L

E

I

L

L

L

K

E

L

L

W

Q

A

A

A

K

E

F

Q

Q

K

K

I

T

V

I

F

I

F

F

I

V

T

S

H

H

S

D

V

L

E

N

E

E

A

A

L

L

F

R

L

I

A

G

T

D

R

R

L

I

I

A

V

I

M

M

binding phosphoaminophosphonic acid-adenylate ester: T39 (= T21), V40 (≠ L22), G41 (≠ A23), G62 (= G44), G64 (= G46), K65 (= K47), D187 (= D160), E188 (= E161)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
37% identity, 73% coverage: 21:209/258 of query aligns to 39:236/382 of 7aheC

query
sites
7aheC

T

T

L

V

A

G

L

V

S

Y

Q

D

V

T

N

N

L

F

T

E

M

I

E

N

R

E

G

G

D

E

F

I

V

F

V

V

A

I

L

M

G

G

A

L

S

S

G

G

C

S

G

G

K

K

T

S

T

T

L

L

S

R

C

L

I

L

A

N

G

R

F

L

M

I

Q

E

P

P

S

T

E

S

G

G

E

K

I

I

R

F

L

I

D

D

G

N

K

Q

S

D

V

V

H

A

G

T

P

L

G

N

A

K

E

E

R

D

-

L

-

Q

-

V

-

R

-

K

-

T

-

M

G

S

V

M

V

V

F

F

Q

Q

K

N

H

F

A

G

L

L

M

F

P

P

W

H

L

R

N

T

V

I

L

L

D

E

N

N

V

T

A

E

L

Y

G

G

L

L

R

E

L

V

R

Q

G

N

V

V

P

P

R

K

A

E

E

E

R

R

L

R

R

K

I

R

A

A

Q

E

E

K

K

A

L

L

A

D

L

N

V

A

G

N

L

L

E

L

K

D

V

F

A

K

A

D

K

Q

P

Y

V

P

Y

K

Q

Q

L

L

S

S

G

G

M

M

Q

Q

R

R

V

V

G

G

I

L

A

A

R

R

T

A

L

L

A

A

N

N

D

D

P

P

A

E

V

I

M

L

L

L

M

M

D

D

E

E

P

A

L

F

G

S

A

A

L

L

D

D

A

P

L

L

T

I

R

R

E

R

T

E

I

M

Q

Q

A

D

L

E

I

L

L

L

K

E

L

L

W

Q

A

A

A

K

E

F

Q

Q

K

K

I

T

V

I

F

I

F

F

I

V

T

S

H

H

S

D

V

L

E

N

E

E

A

A

L

L

F

R

L

I

A

G

T

D

R

R

L

I

I

A

V

I

M

M

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7ahdC Opua (e190q) occluded (see paper)
37% identity, 73% coverage: 21:209/258 of query aligns to 39:236/260 of 7ahdC

query
sites
7ahdC

T
|
T

L

V

A

G

L

V

S

Y

Q

D

V

T

N

N

L

F

T

E

M

I

E

N

R

E

G

G

D

E

F

I

V

F

V

V

A

I

L

M

G

G

A

L

S
|
S

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

C

L

I

L

A

N

G

R

F

L

M

I

Q

E

P

P

S

T

E

S

G

G

E

K

I

I

R

F

L

I

D

D

G

N

K

Q

S

D

V

V

H

A

G

T

P

L

G

N

A

K

E

E

R

D

-

L

-

Q

-

V

-

R

-

K

-

T

-

M

G

S

V

M

V

V

F

F

Q
|
Q

K

N

H

F

A

G

L

L

M

F

P

P

W

H

L

R

N

T

V

I

L

L

D

E

N

N

V

T

A

E

L

Y

G

G

L

L

R

E

L

V

R

Q

G

N

V

V

P

P

R

K

A

E

E

E

R

R

L

R

R

K

I

R

A

A

Q

E

E

K

K

A

L

L

A

D

L

N

V

A

G

N

L

L

E

L

K

D

V

F

A

K

A

D

K

Q

P

Y

V

P

Y
x
K

Q
|
Q

L

L

S
|
S

G

G

G
|
G

M
|
M

Q

Q

R

R

V

V

G

G

I

L

A

A

R

R

T

A

L

L

A

A

N

N

D

D

P

P

A

E

V

I

M

L

L

L

M

M

D

D

E
x
Q

P

A

L

F

G

S

A

A

L

L

D

D

A

P

L

L

T

I

R

R

E

R

T

E

I

M

Q

Q

A

D

L

E

I

L

L

L

K

E

L

L

W

Q

A

A

A

K

E

F

Q

Q

K

K

I

T

V

I

F

I

F

F

I

V

T

S

H
|
H

S

D

V

L

E

N

E

E

A

A

L

L

F

R

L

I

A

G

T

D

R

R

L

I

I

A

V

I

M

M

binding adenosine-5'-triphosphate: T39 (= T21), S61 (= S43), G62 (= G44), G64 (= G46), K65 (= K47), S66 (≠ T48), T67 (= T49), Q111 (= Q84), K161 (≠ Y134), Q162 (= Q135), S164 (= S137), G166 (= G139), M167 (= M140), Q188 (≠ E161), H221 (= H194)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
37% identity, 81% coverage: 1:210/258 of query aligns to 4:217/375 of 2d62A

query
sites
2d62A

M

M

A

A

R

E

L

V

E

K

I

L

D

I

N

N

L

I

S

W

V

K

N

R

Y

F

G

G

G

-

R

-

G

-

A

-

D

D

T

V

L

T

A

A

L

V

S

K

Q

D

V

L

N

S

L

L

T

E

M

I

E

K

R

D

G

G

D

E

F

F

V

L

V

V

A

L

L

L

G

G

A

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

T

L

L

S

R

C

M

I

I

A

A

G

G

F

L

M

E

Q

E

P

P

S

T

E

R

G

G

E

Q

I

I

R

Y

L

I

D

E

G

D

-

N

-

L

-

V

-

A

-

D

-

P

-

E

K

K

S

G

V

V

H

F

G

V

P

P

G

P

A

K

E

E

R

R

G

D

V

V

-

A

-

M

V

V

F

F

Q

Q

K

S

H

Y

A

A

L

L

M

Y

P

P

W

H

L

M

N

T

V

V

L

Y

D

D

N

N

V

I

A

A

L

F

G

P

L

L

R

K

L

L

R

R

G

K

V

V

P

P

R

K

A

Q

E

E

R

I

L

D

R

K

I

R

A

V

Q

R

E

E

K

V

L

A

A

E

L

M

V

L

G

G

L

L

E

T

K

E

V

L

A

L

A

N

K

R

P

K

V

P

Y

R

Q

E

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

I

L

A

G

R

R

T

A

L

I

A

I

N

R

D

R

P

P

A

K

V

V

M

F

L

L

M

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

A

A

L

K

T

L

R

R

E

V

T

K

I

M

Q

R

A

A

L

E

I

L

L

K

K

K

L

L

W

Q

A

R

A

Q

E

L

Q

G

K

V

I

T

V

T

F

I

F

Y

I

V

T

T

H

H

S

D

V

Q

E

V

E

E

A

A

L

M

F

T

L

M

A

G

T

D

R

R

L

I

I

A

V

V

M

M

T

N

binding pyrophosphate 2-: G42 (= G44), C43 (= C45), G44 (= G46), K45 (= K47), T46 (= T48), T47 (= T49)

1g291 Malk (see paper)
38% identity, 74% coverage: 19:210/258 of query aligns to 14:214/372 of 1g291

query
sites
1g291

A

G

D

E

T

V

L

T

A

A

L

V

S

R

Q

E

V

M

N

S

L

L

T

E

M

V

E

K

R

D

G

G

D

E

F

F

V

M

V

I

A

L

L

L

G

G

A

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

T

L

L

S

R

C

M

I

I

A

A

G

G

F

L

M

E

Q

E

P

P

S

S

E

R

G

G

E

Q

I

I

R

Y

L

I

D

G

G

D

K

K

S

L

V

V

H

A

G
x
D

P

P

G

-

A

-

E
|
E

R
x
K

G

G

V

I

-

F

-

V

-

P

-
x
K

-
x
D

-

R

-

D

-

I

-

A

-

M

V

V

F

F

Q

Q

K

S

H

Y

A

A

L

L

M

Y

P

P

W

H

L

M

N

T

V

V

L

Y

D

D

N

N

V

I

A

A

L

F

G

P

L

L

R

K

L

L

R

R

G

K

V

V

P

P

R

R

A

Q

E

E

R

I

L

D

R

Q

I

R

A

V

Q

R

E

E

K

V

L

A

A

E

L

L

V

L

G

G

L

L

E

T

K

E

V

L

A

L

A

N

K

R

P

K

V

P

Y

R

Q

E

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

I

L

A

G

R

R

T

A

L

I

A

V

N

R

D

K

P

P

A

Q

V

V

M

F

L

L

M

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

A

A

L

K

T

L

R

R

E

V

T

R

I

M

Q

R

A

A

L

E

I

L

L

K

K

K

L

L

W

Q

A

R

A

Q

E

L

Q

G

K

V

I

T

V

T

F

I

F

Y

I

V

T

T

H

H

S

D

V

Q

E

V

E

E

A

A

L

M

F

T

L

M

A

G

T

D

R

R

L

I

I

A

V

V

M

M

T

N

binding magnesium ion: D69 (≠ G74), E71 (= E78), K72 (≠ R79), K79 (vs. gap), D80 (vs. gap)
binding pyrophosphate 2-: S38 (= S43), G39 (= G44), C40 (= C45), G41 (= G46), K42 (= K47), T43 (= T48), T44 (= T49)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
37% identity, 89% coverage: 1:230/258 of query aligns to 1:231/371 of P68187

query
sites
P68187

M

M

A

A

R

S

L

V

E

Q

I

L

D

Q

N

N

L

V

S

T

V

K

N

A

Y

W

G

G

G

-

R

-

G

-

A

-

D

E

T

V

L

V

A

V

L

S

S

K

Q

D

V

I

N

N

L

L

T

D

M

I

E

H

R

E

G

G

D

E

F

F

V

V

A

F

L

V

G

G

A

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

L

L

S

R

C

M

I

I

A

A

G

G

F

L

M

E

Q

T

P

I

S

T

E

S

G

G

E

D

I

L

R

F

L

I

D

G

G

E

K

K

S

R

V

M

H

N

G

D

-

T

P

P

G

P

A

A

E

E

R

R

G

G

V

V

-

G

-

M

V

V

F

F

Q

Q

K

S

H

Y

A
|
A

L

L

M

Y

P

P

W

H

L

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

L

F

G

G

L

L

R

K

L

L

R

A

G

G

V

A

P
x
K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

L
x
V

R

N

I

Q

A
x
V

Q

A

E
|
E

K

V

L

L

A

Q

L

L

V
x
A

G

H

L

L

E

-

K

-

V

L

A

D

A

R

K

K

P

P

V

-

Y

K

Q

A

L

L

S

S

G

G

G
|
G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

I

I

A

G

R

R

T

T

L

L

A

V

N

A

D

E

P

P

A

S

V

V

M

F

L

L

M

L

D
|
D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

A

A

L

A

T

L

R

R

E

V

T

Q

I

M

Q

R

A

I

L

E

I

I

L

S

K

R

L

L

W

H

A

K

A

R

E

L

Q

G

K

R

I

T

V

M

F

I

F

Y

I

V

T

T

H

H

S

D

V

Q

E

V

E

E

A

A

L

M

F

T

L

L

A

A

T

D

R

K

L

I

I

V

V

V

M

L

T

-

P

-

S

D

P

A

G

G

R

R

I

V

A

A

H

Q

T

V

-

G

-

K

-

P

-

L

-

E

-

L

Y

Y

E

H

L

Y

P

P

F

-

A

A

R

D

R
|
R

F

F

I

V

A

A

A85 (= A87) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ P108) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ L113) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A116) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E118) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ V123) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G139) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D160) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (= R227) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
37% identity, 89% coverage: 2:230/258 of query aligns to 1:230/374 of 2awnB

query
sites
2awnB

A

A

R

S

L

V

E

Q

I

L

D

Q

N

N

L

V

S

T

V

K

N

A

Y
x
W

G

G

G

-

R

-

G

-

A

-

D

E

T

V

L

V

A
x
V

L

S

S

K

Q

D

V

I

N

N

L

L

T

D

M

I

E

H

R

E

G

G

D

E

F

F

V

V

A

F

L

V

G

G

A

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

C

M

I

I

A

A

G

G

F

L

M

E

Q

T

P

I

S

T

E

S

G

G

E

D

I

L

R

F

L

I

D

G

G

E

K

K

S

R

V

M

H

N

G

D

-

T

P

P

G

P

A

A

E

E

R

R

G

G

V

V

-

G

-

M

V

V

F

F

Q

Q

K

S

H

Y

A

A

L

L

M

Y

P

P

W

H

L

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

L

F

G

G

L

L

R

K

L

L

R

A

G

G

V

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

L

V

R

N

I

Q

A

V

Q

A

E

E

K

V

L

L

A

Q

L

L

V

A

G

H

L

L

E

-

K

-

V

L

A

D

A

R

K

K

P

P

V

-

Y

K

Q

A

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

I

I

A

G

R

R

T

T

L

L

A

V

N

A

D

E

P

P

A

S

V

V

M

F

L

L

M

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

A

A

L

A

T

L

R

R

E

V

T

Q

I

M

Q

R

A

I

L

E

I

I

L

S

K

R

L

L

W

H

A

K

A

R

E

L

Q

G

K

R

I

T

V

M

F

I

F

Y

I

V

T

T

H

H

S

D

V

Q

E

V

E

E

A

A

L

M

F

T

L

L

A

A

T

D

R

K

L

I

I

V

V

V

M

L

T

-

P

-

S

D

P

A

G

G

R

R

I

V

A

A

H

Q

T

V

-

G

-

K

-

P

-

L

-

E

-

L

Y

Y

E

H

L

Y

P

P

F

-

A

A

R

D

R

R

F

F

I

V

A

A

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ A23), S37 (= S43), G38 (= G44), G40 (= G46), K41 (= K47), S42 (≠ T48), T43 (= T49)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
37% identity, 89% coverage: 2:230/258 of query aligns to 1:230/371 of 3puyA

query
sites
3puyA

A

A

R

S

L

V

E

Q

I

L

D

Q

N

N

L

V

S

T

V

K

N

A

Y
x
W

G

G

G

-

R

-

G

-

A

-

D

E

T

V

L

V

A

V

L

S

S

K

Q

D

V

I

N

N

L

L

T

D

M

I

E

H

R

E

G

G

D

E

F

F

V

V

A

F

L

V

G

G

A

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

C

M

I

I

A

A

G

G

F

L

M

E

Q

T

P

I

S

T

E

S

G

G

E

D

I

L

R

F

L

I

D

G

G

E

K

K

S

R

V

M

H

N

G

D

-

T

P

P

G

P

A

A

E

E

R

R

G

G

V

V

-

G

-

M

V

V

F

F

Q
|
Q

K

S

H

Y

A

A

L

L

M

Y

P

P

W

H

L

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

L

F

G

G

L

L

R

K

L

L

R

A

G

G

V

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

L

V

R

N

I

Q

A

V

Q

A

E

E

K

V

L

L

A

Q

L

L

V

A

G

H

L

L

E

-

K

-

V

L

A

D

A
x
R

K

K

P

P

V

-

Y

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

M
x
Q

Q

R

Q

Q

R

R

V

V

G

A

I

I

A

G

R

R

T

T

L

L

A

V

N

A

D

E

P

P

A

S

V

V

M

F

L

L

M

L

D

D

E
|
E

P

P

L

L

G

S

A

N

L

L

D

D

A

A

L

A

T

L

R

R

E

V

T

Q

I

M

Q

R

A

I

L

E

I

I

L

S

K

R

L

L

W

H

A

K

A

R

E

L

Q

G

K

R

I

T

V

M

F

I

F

Y

I

V

T

T

H
|
H

S

D

V

Q

E

V

E

E

A

A

L

M

F

T

L

L

A

A

T

D

R

K

L

I

I

V

V

V

M

L

T

-

P

-

S

D

P

A

G

G

R

R

I

V

A

A

H

Q

T

V

-

G

-

K

-

P

-

L

-

E

-

L

Y

Y

E

H

L

Y

P

P

F

-

A

A

R

D

R

R

F

F

I

V

A

A

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y13), S37 (= S43), G38 (= G44), C39 (= C45), G40 (= G46), K41 (= K47), S42 (≠ T48), T43 (= T49), Q81 (= Q84), R128 (≠ A130), A132 (≠ Q135), S134 (= S137), G136 (= G139), Q137 (≠ M140), E158 (= E161), H191 (= H194)
binding magnesium ion: S42 (≠ T48), Q81 (= Q84)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
37% identity, 89% coverage: 2:230/258 of query aligns to 1:230/371 of 3puxA

query
sites
3puxA

A

A

R

S

L

V

E

Q

I

L

D

Q

N

N

L

V

S

T

V

K

N

A

Y
x
W

G

G

G

-

R

-

G

-

A

-

D

E

T

V

L

V

A

V

L

S

S

K

Q

D

V

I

N

N

L

L

T

D

M

I

E

H

R

E

G

G

D

E

F

F

V

V

A

F

L

V

G

G

A

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

C

M

I

I

A

A

G

G

F

L

M

E

Q

T

P

I

S

T

E

S

G

G

E

D

I

L

R

F

L

I

D

G

G

E

K

K

S

R

V

M

H

N

G

D

-

T

P

P

G

P

A

A

E

E

R

R

G

G

V

V

-

G

-

M

V

V

F

F

Q
|
Q

K

S

H

Y

A

A

L

L

M

Y

P

P

W

H

L

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

L

F

G

G

L

L

R

K

L

L

R

A

G

G

V

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

L

V

R

N

I

Q

A

V

Q

A

E

E

K

V

L

L

A

Q

L

L

V

A

G

H

L

L

E

-

K

-

V

L

A

D

A
x
R

K

K

P

P

V

-

Y

K

Q

A

L

L

S
|
S

G

G

G
|
G

M
x
Q

Q

R

Q

Q

R

R

V

V

G

A

I

I

A

G

R

R

T

T

L

L

A

V

N

A

D

E

P

P

A

S

V

V

M

F

L

L

M

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

A

A

L

A

T

L

R

R

E

V

T

Q

I

M

Q

R

A

I

L

E

I

I

L

S

K

R

L

L

W

H

A

K

A

R

E

L

Q

G

K

R

I

T

V

M

F

I

F

Y

I

V

T

T

H
|
H

S

D

V

Q

E

V

E

E

A

A

L

M

F

T

L

L

A

A

T

D

R

K

L

I

I

V

V

V

M

L

T

-

P

-

S

D

P

A

G

G

R

R

I

V

A

A

H

Q

T

V

-

G

-

K

-

P

-

L

-

E

-

L

Y

Y

E

H

L

Y

P

P

F

-

A

A

R

D

R

R

F

F

I

V

A

A

binding adenosine-5'-diphosphate: W12 (≠ Y13), G38 (= G44), C39 (= C45), G40 (= G46), K41 (= K47), S42 (≠ T48), T43 (= T49), R128 (≠ A130), S134 (= S137), Q137 (≠ M140)
binding beryllium trifluoride ion: S37 (= S43), G38 (= G44), K41 (= K47), Q81 (= Q84), S134 (= S137), G136 (= G139), H191 (= H194)
binding magnesium ion: S42 (≠ T48), Q81 (= Q84)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
37% identity, 89% coverage: 2:230/258 of query aligns to 1:230/371 of 3puwA

query
sites
3puwA

A

A

R

S

L

V

E

Q

I

L

D

Q

N

N

L

V

S

T

V

K

N

A

Y
x
W

G

G

G

-

R

-

G

-

A

-

D

E

T

V

L

V

A
x
V

L

S

S

K

Q

D

V

I

N

N

L

L

T

D

M

I

E

H

R

E

G

G

D

E

F

F

V

V

A

F

L

V

G

G

A

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

C

M

I

I

A

A

G

G

F

L

M

E

Q

T

P

I

S

T

E

S

G

G

E

D

I

L

R

F

L

I

D

G

G

E

K

K

S

R

V

M

H

N

G

D

-

T

P

P

G

P

A

A

E

E

R

R

G

G

V

V

-

G

-

M

V

V

F

F

Q
|
Q

K

S

H

Y

A

A

L

L

M

Y

P

P

W

H

L

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

L

F

G

G

L

L

R

K

L

L

R

A

G

G

V

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

L

V

R

N

I

Q

A

V

Q

A

E

E

K

V

L

L

A

Q

L

L

V

A

G

H

L

L

E

-

K

-

V

L

A

D

A
x
R

K

K

P

P

V

-

Y

K

Q
x
A

L

L

S
|
S

G
|
G

M
x
Q

Q

R

Q

Q

R

R

V

V

G

A

I

I

A

G

R

R

T

T

L

L

A

V

N

A

D

E

P

P

A

S

V

V

M

F

L

L

M

L

D

D

E
|
E

P

P

L

L

G

S

A

N

L

L

D

D

A

A

L

A

T

L

R

R

E

V

T

Q

I

M

Q

R

A

I

L

E

I

I

L

S

K

R

L

L

W

H

A

K

A

R

E

L

Q

G

K

R

I

T

V

M

F

I

F

Y

I

V

T

T

H
|
H

S

D

V

Q

E

V

E

E

A

A

L

M

F

T

L

L

A

A

T

D

R

K

L

I

I

V

V

V

M

L

T

-

P

-

S

D

P

A

G

G

R

R

I

V

A

A

H

Q

T

V

-

G

-

K

-

P

-

L

-

E

-

L

Y

Y

E

H

L

Y

P

P

F

-

A

A

R

D

R

R

F

F

I

V

A

A

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ A23), G38 (= G44), C39 (= C45), G40 (= G46), K41 (= K47), S42 (≠ T48), T43 (= T49), R128 (≠ A130), A132 (≠ Q135), S134 (= S137), Q137 (≠ M140)
binding tetrafluoroaluminate ion: S37 (= S43), G38 (= G44), K41 (= K47), Q81 (= Q84), S134 (= S137), G135 (= G138), G136 (= G139), E158 (= E161), H191 (= H194)
binding magnesium ion: S42 (≠ T48), Q81 (= Q84)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
37% identity, 89% coverage: 2:230/258 of query aligns to 1:230/371 of 3puvA

query
sites
3puvA

A

A

R

S

L

V

E

Q

I

L

D

Q

N

N

L

V

S

T

V

K

N

A

Y
x
W

G

G

G

-

R

-

G

-

A

-

D

E

T

V

L

V

A
x
V

L

S

S

K

Q

D

V

I

N

N

L

L

T

D

M

I

E

H

R

E

G

G

D

E

F

F

V

V

A

F

L

V

G

G

A

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

C

M

I

I

A

A

G

G

F

L

M

E

Q

T

P

I

S

T

E

S

G

G

E

D

I

L

R

F

L

I

D

G

G

E

K

K

S

R

V

M

H

N

G

D

-

T

P

P

G

P

A

A

E

E

R

R

G

G

V

V

-

G

-

M

V

V

F

F

Q
|
Q

K

S

H

Y

A

A

L

L

M

Y

P

P

W

H

L

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

L

F

G

G

L

L

R

K

L

L

R

A

G

G

V

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

L

V

R

N

I

Q

A

V

Q

A

E

E

K

V

L

L

A

Q

L

L

V

A

G

H

L

L

E

-

K

-

V

L

A

D

A
x
R

K

K

P

P

V

-

Y

K

Q
x
A

L

L

S
|
S

G

G

M
x
Q

Q

R

Q

Q

R

R

V

V

G

A

I

I

A

G

R

R

T

T

L

L

A

V

N

A

D

E

P

P

A

S

V

V

M

F

L

L

M

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

A

A

L

A

T

L

R

R

E

V

T

Q

I

M

Q

R

A

I

L

E

I

I

L

S

K

R

L

L

W

H

A

K

A

R

E

L

Q

G

K

R

I

T

V

M

F

I

F

Y

I

V

T

T

H

H

S

D

V

Q

E

V

E

E

A

A

L

M

F

T

L

L

A

A

T

D

R

K

L

I

I

V

V

V

M

L

T

-

P

-

S

D

P

A

G

G

R

R

I

V

A

A

H

Q

T

V

-

G

-

K

-

P

-

L

-

E

-

L

Y

Y

E

H

L

Y

P

P

F

-

A

A

R

D

R

R

F

F

I

V

A

A

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ A23), G38 (= G44), C39 (= C45), G40 (= G46), K41 (= K47), S42 (≠ T48), T43 (= T49), R128 (≠ A130), A132 (≠ Q135), S134 (= S137), Q137 (≠ M140)
binding magnesium ion: S42 (≠ T48), Q81 (= Q84)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
37% identity, 89% coverage: 1:230/258 of query aligns to 1:231/369 of P19566

query
sites
P19566

M

M

A

A

R

S

L

V

E

Q

I

L

D

R

N

N

L

V

S

T

V

K

N

A

Y

W

G

G

G

-

R

-

G

-

A

-

D

D

T

V

L

V

A

V

L

S

S

K

Q

D

V

I

N

N

L

L

T

D

M

I

E

H

R

D

G

G

D

E

F

F

V

V

A

F

L

V

G

G

A

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

L

L

S

R

C

M

I

I

A

A

G

G

F

L

M

E

Q

T

P

I

S

T

E

S

G

G

E

D

I

L

R

F

L

I

D

G

G

E

K

T

S

R

V

M

H

N

G

D

-

I

P

P

G

P

A

A

E

E

R

R

G

G

V

V

-

G

-

M

V

V

F

F

Q

Q

K

S

H

Y

A

A

L
|
L

M

Y

P

P

W

H

L

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

L

F

G

G

L

L

R

K

L

L

R

A

G

G

V

A

P

K

R

K

A

E

-

V

-

M

-

N

E

Q

R

R

L

V

R

N

I

Q

A

V

Q

A

E

E

K

V

L

L

A

Q

L

L

V

A

G

H

L

L

E

-

K

-

V

L

A

E

A

R

K

K

P

P

V

-

Y

K

Q

A

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

I

I

A

G

R

R

T

T

L

L

A

V

N

A

D

E

P

P

A

R

V

V

M

F

L

L

M

L

D

D

E

E

P
|
P

L

L

G

S

A

N

L

L

D
|
D

A

A

L

A

T

L

R

R

E

V

T

Q

I

M

Q

R

A

I

L

E

I

I

L

S

K

R

L

L

W

H

A

K

A

R

E

L

Q

G

K

R

I

T

V

M

F

I

F

Y

I

V

T

T

H

H

S

D

V

Q

E

V

E

E

A

A

L

M

F

T

L

L

A

A

T

D

R

K

L

I

I

V

V

V

M

L

T

-

P

-

S

D

P

A

G

G

R

R

I

V

A

A

H

Q

T

V

-

G

-

K

-

P

-

L

-

E

-

L

Y

Y

E

H

L

Y

P

P

F

-

A

A

R

D

R

R

F

F

I

V

A

A

L86 (= L88) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P162) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D167) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
37% identity, 88% coverage: 4:230/258 of query aligns to 1:228/367 of 1q12A

query
sites
1q12A

L

V

E

Q

I

L

D

Q

N

N

L

V

S

T

V

K

N

A

Y
x
W

G

G

G

-

R

-

G

-

A

-

D

E

T

V

L

V

A

V

L

S

S

K

Q

D

V

I

N

N

L

L

T

D

M

I

E

H

R

E

G

G

D

E

F

F

V

V

A

F

L

V

G

G

A

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

C

M

I

I

A

A

G

G

F

L

M

E

Q

T

P

I

S

T

E

S

G

G

E

D

I

L

R

F

L

I

D

G

G

E

K

K

S

R

V

M

H

N

G

D

-

T

P

P

G

P

A

A

E

E

R

R

G

G

V

V

-

G

-

M

V

V

F

F

Q

Q

K

S

H

Y

A

A

L

L

M

Y

P

P

W

H

L

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

L

F

G

G

L

L

R

K

L

L

R

A

G

G

V

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

L

V

R

N

I

Q

A

V

Q

A

E

E

K

V

L

L

A

Q

L

L

V

A

G

H

L

L

E

-

K

-

V

L

A

D

A
x
R

K

K

P

P

V

-

Y

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

M
x
Q

Q

R

Q

Q

R

R

V

V

G

A

I

I

A

G

R

R

T

T

L

L

A

V

N

A

D

E

P

P

A

S

V

V

M

F

L

L

M

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

A

A

L

A

T

L

R

R

E

V

T

Q

I

M

Q

R

A

I

L

E

I

I

L

S

K

R

L

L

W

H

A

K

A

R

E

L

Q

G

K

R

I

T

V

M

F

I

F

Y

I

V

T

T

H

H

S

D

V

Q

E

V

E

E

A

A

L

M

F

T

L

L

A

A

T

D

R

K

L

I

I

V

V

V

M

L

T

-

P

-

S

D

P

A

G

G

R

R

I

V

A

A

H

Q

T

V

-

G

-

K

-

P

-

L

-

E

-

L

Y

Y

E

H

L

Y

P

P

F

-

A

A

R

D

R

R

F

F

I

V

A

A

binding adenosine-5'-triphosphate: W10 (≠ Y13), S35 (= S43), G36 (= G44), C37 (= C45), G38 (= G46), K39 (= K47), S40 (≠ T48), T41 (= T49), R126 (≠ A130), A130 (≠ Q135), S132 (= S137), G134 (= G139), Q135 (≠ M140)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
41% identity, 69% coverage: 23:201/258 of query aligns to 20:211/232 of 1f3oA

query
sites
1f3oA

A

A

L

L

S

K

Q

N

V

V

N

N

L

L

T

N

M

I

E

K

R

E

G

G

D

E

F

F

V

V

V

S

A

I

L

M

G

G

A

P

S

S

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

L

M

L

L

S

N

C

I

I

I

A

G

G

C

F

L

M

D

Q

K

P

P

S

T

E

E

G

G

E

E

I

V

R

Y

L

I

D

D

G

N

K

I

S

K

V

T

H

N

G

D

P

L

G

D

A

D

E

D

R

E

-

L

-

T

-

K

-

I

-

R

-

D

-

K

-

I

G

G

V

F

V

V

F

F

Q

Q

K

Q

H

F

A

N

L

L

M

I

P

P

W

L

L

L

N

T

V

A

L

L

D

E

N

N

V

V

A

E

L

L

G

P

L

L

-

I

-

F

R

K

L

Y

R

R

G

G

-

A

V

M

P

S

R

G

A

E

E

E

R

R

L

R

R

K

I

R

A

A

Q

L

E

E

K

C

L

L

A

K

L

M

V

A

G

E

L

L

E

E

-

E

K

R

V

F

A

A

A

N

K

H

P

K

V

P

Y

N

Q

Q

L

L

S

S

G

G

M

Q

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

T

A

L

L

A

A

N

N

D

N

P

P

A

P

V

I

M

I

L

L

M

A

D

D

E

E

P

P

L

T

G

G

A

A

L

L

D

D

A

S

L

K

T

T

R

G

E

E

T

K

I

I

Q

M

A

Q

L

L

I

L

L

K

K

K

L

L

W

N

A

E

E

D

Q

G

K

K

I

T

V

V

F

V

I

V

T

T

H

H

S

D

V

I

E

N

E

V

A

A

L

R

F

F

binding adenosine-5'-diphosphate: G41 (= G44), G43 (= G46), K44 (= K47), S45 (≠ T48), T46 (= T49)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
41% identity, 84% coverage: 2:219/258 of query aligns to 2:219/350 of 3fvqB

query
sites
3fvqB

A

A

R

A

L

L

E

H

I

I

D

G

N

H

L

L

S

S

V

K

N

S

Y
x
F

G

-

R

-

G

-

A
x
Q

D

N

T
|
T

L

P

A
x
V

L

L

S

N

Q

D

V

I

N

S

L

L

T

S

M

L

E

D

R

P

G

G

D

E

F

I

V

L

V

F

A

I

L

I

G

G

A

A

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

L

S

R

C

C

I

L

A

A

G

G

F

F

M

E

Q

Q

P

P

S

D

E

S

G

G

E

E

I

I

R

S

L

L

D

S

G

G

K

K

S

T

V

I

H

F

G

S

-

K

-

N

-

T

-

N

-

L

P

P

G

V

A

R

E

E

R

R

-

R

-

L

G

G

V

Y

V

L

F

V

Q
|
Q

K

E

H

G

A

V

L

L

M

F

P

P

W

H

L

L

N

T

V

V

L

Y

D

R

N

N

V

I

A

A

L

Y

G

G

L

L

-

G

R

N

L

G

R

K

G

G

V

R

P

T

R

A

A

Q

E

E

R

R

L

Q

R

R

I

I

A

-

Q

E

E

A

K

M

L

L

A

E

L

L

V

T

G

G

L

I

E

S

K

E

V

L

A

A

A

G

K
x
R

P

Y

V

P

Y

H

Q
x
E

L

L

S
|
S

G

G

G
|
G

M
x
Q

Q

Q

R

R

V

A

G

A

I

L

A

A

R

R

T

A

L

L

A

A

N

P

D

D

P

P

A

E

V

L

M

I

L

L

M

L

D

D

E

E

P

P

L

F

G

S

A

A

L

L

D

D

A

E

L

Q

T

L

R

R

E

R

T

Q

I

I

Q

R

A

E

L

D

I

M

L

I

K

A

L

A

W

L

A

R

A

A

E

N

Q

G

K

K

I

S

V

A

F

V

F

F

I

V

T

S

H

H

S

D

V

R

E

E

A

A

L

L

F

Q

L

Y

A

A

T

D

R

R

L

I

I

A

V

V

M

M

T

-

P

-

S

K

P

Q

G

G

R

R

I

I

A

L

H

Q

T

T

binding adenosine-5'-triphosphate: F13 (≠ Y13), Q14 (≠ A19), T16 (= T21), V18 (≠ A23), S38 (= S43), G39 (= G44), C40 (= C45), G41 (= G46), K42 (= K47), T43 (= T48), T44 (= T49), R133 (≠ K131), E137 (≠ Q135), S139 (= S137), G141 (= G139), Q142 (≠ M140)
binding calcium ion: T43 (= T48), Q86 (= Q84)

3d31A Modbc from methanosarcina acetivorans (see paper)
32% identity, 95% coverage: 4:247/258 of query aligns to 2:249/348 of 3d31A

query
sites
3d31A

L

I

E

E

I

I

D

E

N

S

L

L

S

S

V

R

N

K

Y

W

G

-

R

-

G

-

A

-

D

K

T

N

L

F

A

S

L

L

S

D

Q

N

V

L

N

S

L

L

T

K

M

V

E

E

R

S

G

G

D

E

F

Y

V

F

V

V

A

I

L

L

G

G

A

P

S

T

G

G

C

A

G

G

K

K

T

T

T

L

L

F

L

L

S

E

C

L

I

I

A

A

G

G

F

F

M

H

Q

V

P

P

S

D

E

S

G

G

E

R

I

I

R

L

L

L

D

D

G

G

K

K

S

D

V

V

H

T

G

D

P

L

G

S

A

P

E

E

R

K

-

H

-

D

-

I

G

A

V

F

V

V

F

Y

Q

Q

K

N

H

Y

A

S

L

L

M

F

P

P

W

H

L

M

N

N

V

V

L

K

D

K

N

N

V

L

A

E

L

F

G

G

L

M

R

R

L

M

R

K

G

K

V

I

P

K

R

D

A

P

E

K

R

R

L

V

R

L

I

D

A

T

Q

A

E

R

K

D

L

L

A

K

L

I

V

-

G

-

L

-

E

E

K

H

V

L

A

L

A

D

K

R

P

N

V

P

Y

L

Q

T

L

L

S

S

G

G

M

E

Q

Q

R

R

V

V

G

A

I

L

A

A

R

R

T

A

L

L

A

V

N

T

D

N

P

P

A

K

V

I

M

L

L

L

M

L

D

D

E

E

P

P

L

L

G

S

A

A

L

L

D

D

A

P

L

R

T

T

R

Q

E

E

T

N

I

A

Q

R

A

E

L

M

I

L

L

S

K

V

L

L

W

H

A

K

E

N

Q

K

K

L

I

T

V

V

F

L

F

H

I

I

T

T

H

H

S

D

V

Q

E

T

E

E

A

A

L

R

F

I

L

M

A

A

T

D

R

R

L

I

I

A

V

V

M

V

T

M

P

D

S

G

P

K

-

L

-

I

-

Q

-

V

G

G

R

K

I

P

A

E

H

E

T

I

Y

F

E

E

L

K

P

P

F

V

A

E

R

G

R

R

-

V

-

A

-

S

F

F

I

V

A

G

C

F

G

E

N

N

A

V

-

L

-

K

-

G

R

R

E

V

V

I

K

S

A

A

D

E

P

Q

E

G

F

L

I

L

R

R

Y

I

R

R

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
41% identity, 69% coverage: 23:201/258 of query aligns to 20:211/230 of 1l2tA

query
sites
1l2tA

A

A

L

L

S

K

Q

N

V

V

N

N

L

L

T

N

M

I

E

K

R

E

G

G

D

E

F

F

V

V

V

S

A

I

L

M

G

G

A

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

T
x
S

T
|
T

L

M

L

L

S

N

C

I

I

I

A

G

G

C

F

L

M

D

Q

K

P

P

S

T

E

E

G

G

E

E

I

V

R

Y

L

I

D

D

G

N

K

I

S

K

V

T

H

N

G

D

P

L

G

D

A

D

E

D

R

E

-

L

-

T

-

K

-

I

-

R

-

D

-

K

-

I

G

G

V

F

V

V

F

F

Q

Q

K

Q

H

F

A

N

L

L

M

I

P

P

W

L

L

L

N

T

V

A

L

L

D

E

N

N

V

V

A

E

L

L

G

P

L

L

-

I

-

F

R

K

L

Y

R

R

G

G

-

A

V

M

P

S

R

G

A

E

E

E

R

R

L

R

R

K

I

R

A

A

Q

L

E

E

K

C

L

L

A

K

L

M

V

A

G

E

L

L

E

E

-

E

K

R

V
x
F

A

A

A

N

K

H

P

K

V

P

Y

N

Q
|
Q

L

L

S
|
S

G

G

G
|
G

M
x
Q

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

T

A

L

L

A

A

N

N

D

N

P

P

A

P

V

I

M

I

L

L

M

A

D

D

E

Q

P

P

L

T

G

G

A

A

L

L

D

D

A

S

L

K

T

T

R

G

E

E

T

K

I

I

Q

M

A

Q

L

L

I

L

L

K

K

K

L

L

W

N

A

E

E

D

Q

G

K

K

I

T

V

V

F

V

I

V

T

T

H
|
H

S

D

V

I

E

N

E

V

A

A

L

R

F

F

binding adenosine-5'-triphosphate: S40 (= S43), G41 (= G44), S42 (≠ C45), G43 (= G46), K44 (= K47), S45 (≠ T48), T46 (= T49), F138 (≠ V128), Q145 (= Q135), S147 (= S137), G149 (= G139), Q150 (≠ M140), H204 (= H194)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
39% identity, 75% coverage: 17:209/258 of query aligns to 16:215/226 of 5xu1B

query
sites
5xu1B

G

G

G

D

A

Q

D

E

T

L

L

Q

A

V

L

L

S

K

Q

N

V

I

N

N

L

L

T

E

M

V

E

N

R

E

G

G

D

E

F

F

V

V

V

A

A

I

L

M

G

G

A

P

S

S

G

G

C

S

G
|
G

K
|
K

T
x
S

T

T

L

L

L

M

S

N

C

T

I

I

A

G

G

M

F

L

M

D

Q

T

P

P

S

T

E

S

G

G

E

E

I

Y

R

Y

L

L

D

E

G

G

K

Q

S

E

V

V

H

A

G

G

P

L

G

G

A

E

E

K

R

Q

-

L

-

A

-

K

-

V

-

R

-

N

-

Q

-

I

G

G

V

F

V

V

F

F

Q

Q

K

Q

H

F

A

F

L

L

M

L

P

S

W

K

L

L

N

N

V

A

L

L

D

Q

N

N

V

V

A

E

L

L

G

P

L

L

R

I

L

Y

R

A

G

G

V

V

P

S

R

S

A

S

E

K

R

R

L

R

R

K

I

L

A

A

Q

E

E

E

K

Y

L

L

A

D

L

K

V

V

G

E

L

L

E

T

K

E

V

R

A

S

A

H

K

H

P

L

V

P

Y

S

Q

E

L

L

S

S

G

G

M

Q

Q

K

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

T

A

L

L

A

V

N

N

D

N

P

P

A

S

V

I

M

I

L

L

M

A

D

D

E

E

P

P

L

T

G

G

A

A

L

L

D

D

A

T

L

K

T

T

R

G

E

N

T

Q

I

I

Q

M

A

Q

L

L

I

L

L

V

K

D

L

L

W

-

A

N

A

K

E

E

Q

G

K

K

I

T

V

I

F

I

F

M

I

V

T

T

H

H

S

E

V

P

E

E

E

I

A

A

L

A

F

Y

L

-

A

A

T

K

R

R

L

Q

I

I

V

V

M

I

binding magnesium ion: G45 (= G46), K46 (= K47), S47 (≠ T48)

8hprC Lpqy-sugabc in state 4 (see paper)
35% identity, 72% coverage: 23:209/258 of query aligns to 18:207/363 of 8hprC

query
sites
8hprC

A

A

L

V

S

K

Q

E

V

F

N

S

L

M

T

T

M

I

E

A

R

D

G

G

D

E

F

F

V

I

A

L

L

V

G

G

A

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
x
S

T

T

L

T

L

L

S

N

C

M

I

I

A

A

G

G

F

L

M

E

Q

E

P

I

S

T

E

S

G

G

E

E

I

L

R

R

L

I

D

G

G

G

K

E

S

R

V

V

H

N

G

E

P

K

G

A

A

P

E

K

R

D

-

R

-

D

-

I

G

A

V

M

V

V

F

F

Q
|
Q

K

S

H

Y

A

A

L

L

M

Y

P

P

W

H

L

M

N

T

V

V

L

R

D

Q

N

N

V

I

A

A

L

F

G

P

L

L

R

T

L

L

R

A

G

K

V

V

P

P

R

K

A

A

E

E

R

I

L

A

R

A

I

K

A

V

Q

E

E

E

K

T

L

A

A

K

L

I

V

L

G

D

L

L

E

S

K

E

V

L

A

L

A

D

K

R

P

K

V

P

Y

G

Q
|
Q

L

L

S

S

G
|
G

M
x
Q

Q

R

Q

Q

R

R

V

V

G

A

I

M

A

G

R

R

T

A

L

I

A

V

N

R

D

S

P

P

A

K

V

A

M

F

L

L

M

M

D

D

E

Q

P

P

L

L

G

S

A

N

L

L

D

D

A

A

L

K

T

L

R

R

E

V

T

Q

I

M

Q

R

A

A

L

E

I

I

L

S

K

R

L

L