SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS34300 FitnessBrowser__Cup4G11:RR42_RS34300 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

3pvcA Crystal structure of apo mnmc from yersinia pestis (see paper)
28% identity, 59% coverage: 3:253/424 of query aligns to 214:451/635 of 3pvcA

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3pvcA

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binding flavin-adenine dinucleotide: I240 (= I32), G241 (= G33), G242 (= G34), G243 (= G35), I244 (≠ F36), V245 (≠ T37), A265 (= A57), D266 (≠ N58), A273 (= A64), S274 (= S65), N276 (≠ R67), A280 (≠ Q71), E404 (≠ A205), L405 (≠ V206), T434 (= T235), R437 (≠ T239)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 454, 456, 477, 538, 588, 589, 590, 591, 592, 593

3sglA The crystal structure of mnmc from yersinia pestis bound with fad and sam (see paper)
28% identity, 59% coverage: 3:253/424 of query aligns to 211:447/630 of 3sglA

query
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3sglA

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binding flavin-adenine dinucleotide: I237 (= I32), G238 (= G33), G239 (= G34), G240 (= G35), I241 (≠ F36), V242 (≠ T37), A262 (= A57), D263 (≠ N58), A270 (= A64), S271 (= S65), N273 (≠ R67), A277 (≠ Q71), E401 (≠ A205), L402 (≠ V206), T430 (≠ N236)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 450, 473, 534, 583, 584, 585, 586, 587, 588
binding s-adenosylmethionine: 33, 34, 35, 36, 37, 38, 39, 68, 69, 123, 124, 145, 146, 147

Query Sequence

>RR42_RS34300 FitnessBrowser__Cup4G11:RR42_RS34300
MPLPSSLWAATAPPAPPTPALRESLATDVLVIGGGFTGLSTALHLAEGGAHVCVLEANEP
GWGASGRNGGQVNPTLKHDPDELVRLFGAARAEPLLDAVSRSADLVFDLIARHGIDCQPV
RSGWLQLSYSPGAVPAMHARAEQWARRGAPVDVLDRAAVARRVGTQAFAGGWLDGRAGAI
QPLAYTRGLVGAAQRAGATVHGGTAVTALARQGTHWVATTSLGATVTAKQVVIATNGYTG
PLWPGLAQTVLSANSFIVATQPLRDAEAHGILTQGETASTSQRLLLYFRKDALGRLLMGG
RGFFADPRGPQDFAHLERSLELLFPQLGPLSYEYRWAGRIAITRDFMPHVHEPAPGVIMA
LGCNGRGIALCTSLGQHIAARLAGKGGDFPYPITPVAGIPLHGLQRFYIAAGVAWYSLLD
KLGA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory