SitesBLAST
Comparing RR42_RS36645 FitnessBrowser__Cup4G11:RR42_RS36645 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4x28A Crystal structure of the chse4-chse5 complex from mycobacterium tuberculosis (see paper)
34% identity, 100% coverage: 1:402/402 of query aligns to 1:382/386 of 4x28A
- active site: Y122 (= Y128), S123 (= S129), E240 (= E244), G365 (= G385), M377 (≠ K397)
- binding dihydroflavine-adenine dinucleotide: I120 (≠ Q126), Y122 (= Y128), S123 (= S129), G128 (= G134), T129 (≠ S135), W153 (= W159), S155 (≠ T161), F363 (≠ I383), T367 (= T387), E369 (= E389), V370 (= V390)
I6YCA3 Acyl-CoA dehydrogenase FadE26; ACAD; 3-oxocholest-4-en-26-oyl-CoA dehydrogenase alpha subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 100% coverage: 1:402/402 of query aligns to 1:395/400 of I6YCA3
- IGYS 127:130 (≠ QGYS 126:129) binding
- T136 (≠ S135) binding
- S162 (≠ T161) binding
- E247 (= E244) mutation to A: Loss of dehydrogenase activity.
- TNE 380:382 (≠ TTE 387:389) binding
6wy8B Tcur3481-tcur3483 steroid acad (see paper)
34% identity, 88% coverage: 1:354/402 of query aligns to 1:349/384 of 6wy8B
Sites not aligning to the query:
P71858 Acyl-CoA dehydrogenase FadE29; ACAD; 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA dehydrogenase beta subunit; 3-oxo-4-pregnene-20-carboxyl-CoA dehydrogenase beta subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 88% coverage: 1:352/402 of query aligns to 1:353/387 of P71858
- E241 (= E244) mutation to Q: Unable to dehydrogenate pregnene-carboxyl-CoA ester.
6wy9A Tcur3481-tcur3483 steroid acad g363a variant (see paper)
33% identity, 87% coverage: 7:354/402 of query aligns to 3:345/380 of 6wy9A
Sites not aligning to the query:
P96855 Acyl-CoA dehydrogenase FadE34; ACAD; 3-oxochol-4-en-24-oyl-CoA dehydrogenase; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 87% coverage: 54:402/402 of query aligns to 392:709/711 of P96855
- E581 (= E244) mutation to Q: Displays less than 1% activity with cholyl-CoA as substrate. Still binds FAD.
Sites not aligning to the query:
- 236 R→A: Displays less than 2% activity with cholyl-CoA as substrate. Cannot bind FAD.
2pg0A Crystal structure of acyl-coa dehydrogenase from geobacillus kaustophilus
30% identity, 86% coverage: 54:399/402 of query aligns to 49:378/380 of 2pg0A
- active site: M124 (≠ Y128), T125 (≠ S129), E243 (= E244), A364 (≠ G385), R376 (≠ K397)
- binding flavin-adenine dinucleotide: I122 (≠ Q126), M124 (≠ Y128), T125 (≠ S129), G130 (= G134), S131 (= S135), F155 (≠ W159), I156 (≠ T160), T157 (= T161), R269 (≠ A268), F272 (≠ A272), F279 (≠ D279), Q337 (≠ E336), L338 (≠ V337), G340 (= G339), G341 (≠ A342), V359 (≠ K380), I362 (= I383), Y363 (= Y384), T366 (= T387), E368 (= E389), M369 (≠ V390)
P51174 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Mus musculus (Mouse) (see paper)
27% identity, 99% coverage: 5:400/402 of query aligns to 53:427/430 of P51174
- K318 (≠ D270) modified: N6-acetyllysine; mutation to R: Reduces activity by 37%; reduces activity by 80% when associated with R-322.
- K322 (≠ R274) modified: N6-acetyllysine; alternate; mutation to R: Reduces activity by 23%; reduces activity by 80% when associated with R-318.
Sites not aligning to the query:
- 42 modified: N6-acetyllysine; K→R: Reduces activity by 90% when associated with R-318 and R-322.
1ukwB Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
29% identity, 91% coverage: 36:400/402 of query aligns to 29:377/379 of 1ukwB
- active site: L124 (≠ Y128), S125 (= S129), T241 (≠ E244), E362 (≠ G385), R374 (≠ K397)
- binding cobalt (ii) ion: D145 (= D149), H146 (≠ R150)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), S125 (= S129), G130 (= G134), S131 (= S135), W155 (= W159), S157 (≠ T161), K200 (≠ L205), L357 (≠ K380), Y361 (= Y384), E362 (≠ G385), T364 (= T387), E366 (= E389), L370 (≠ N393)
1ukwA Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
29% identity, 91% coverage: 36:400/402 of query aligns to 29:377/379 of 1ukwA
- active site: L124 (≠ Y128), S125 (= S129), T241 (≠ E244), E362 (≠ G385), R374 (≠ K397)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), S125 (= S129), G130 (= G134), S131 (= S135), W155 (= W159), S157 (≠ T161), L357 (≠ K380), Y361 (= Y384), E362 (≠ G385), T364 (= T387), E366 (= E389), L370 (≠ N393)
6fahD Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
27% identity, 99% coverage: 3:400/402 of query aligns to 1:377/379 of 6fahD
- active site: L124 (≠ Y128), T125 (≠ S129), G241 (≠ E244), G374 (≠ K397)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), T125 (≠ S129), R152 (≠ Q156), F155 (≠ W159), T157 (= T161), E198 (≠ V203), R267 (≠ A268), Q269 (≠ D270), F270 (≠ A272), I274 (≠ L276), F277 (≠ D279), Q335 (≠ E336), I336 (≠ V337), G339 (≠ A342), Y361 (= Y384), T364 (= T387), Q366 (≠ E389)
5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
28% identity, 100% coverage: 1:400/402 of query aligns to 1:377/378 of 5ol2F
- active site: L124 (≠ Y128), T125 (≠ S129), G241 (≠ E244), G374 (≠ K397)
- binding calcium ion: E29 (≠ Q36), E33 (≠ K40), R35 (≠ D42)
- binding coenzyme a persulfide: L238 (= L241), R242 (= R245), E362 (≠ G385), G363 (= G386)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), T125 (≠ S129), P127 (= P131), T131 (≠ S135), F155 (≠ W159), I156 (≠ T160), T157 (= T161), E198 (≠ P201), R267 (≠ A268), F270 (≠ A272), L274 (= L276), F277 (≠ D279), Q335 (≠ E336), L336 (≠ V337), G338 (= G339), G339 (≠ A342), Y361 (= Y384), T364 (= T387), E366 (= E389)
8hk0B Crystal structure of fic32-33 complex from streptomyces ficellus nrrl 8067 (see paper)
33% identity, 61% coverage: 5:248/402 of query aligns to 5:247/379 of 8hk0B
Sites not aligning to the query:
4iv6B X-ray crystal structure of an isovaleryl-coa dehydrogenase from mycobacterium smegmatis (see paper)
28% identity, 99% coverage: 5:400/402 of query aligns to 2:376/383 of 4iv6B
- active site: L121 (≠ Y128), T122 (≠ S129), G240 (vs. gap), E361 (vs. gap), K373 (= K397)
- binding dihydroflavine-adenine dinucleotide: L121 (≠ Y128), T122 (≠ S129), G126 (≠ S133), G127 (= G134), S128 (= S135), W152 (= W159), I153 (≠ T160), S154 (≠ T161), R266 (≠ T269), S268 (≠ G271), F269 (≠ A272), I273 (≠ L276), H276 (≠ D279), V279 (≠ M282), R334 (≠ E336), V335 (= V337), G338 (≠ A342), L356 (= L362), G360 (= G385), T363 (= T387), E365 (= E389), I366 (≠ V390)
P28330 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Homo sapiens (Human) (see 2 papers)
25% identity, 99% coverage: 5:400/402 of query aligns to 53:427/430 of P28330
- E291 (= E244) active site, Proton acceptor; mutation to Q: Loss of long-chain-acyl-CoA dehydrogenase activity. No effect on protein abundance. No effect on solubility. No effect on substrate binding.
- S303 (≠ R256) to T: in dbSNP:rs1801204
- K333 (= K285) to Q: in dbSNP:rs2286963
4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
25% identity, 100% coverage: 1:400/402 of query aligns to 1:378/380 of 4l1fA
- active site: L125 (≠ Y128), T126 (≠ S129), G242 (≠ E244), E363 (≠ G385), R375 (≠ K397)
- binding coenzyme a persulfide: T132 (≠ S135), H179 (≠ E183), F232 (≠ W234), M236 (≠ K238), E237 (≠ Y239), L239 (= L241), D240 (≠ G242), R243 (= R245), Y362 (= Y384), E363 (≠ G385), G364 (= G386), R375 (≠ K397)
- binding flavin-adenine dinucleotide: F123 (≠ Q126), L125 (≠ Y128), T126 (≠ S129), G131 (= G134), T132 (≠ S135), F156 (≠ W159), I157 (≠ T160), T158 (= T161), R268 (= R283), Q270 (≠ K285), F271 (≠ I286), I275 (≠ E290), F278 (≠ I293), L281 (= L296), Q336 (≠ E351), I337 (vs. gap), G340 (= G353), I358 (≠ K380), Y362 (= Y384), T365 (= T387), Q367 (≠ E389)
- binding 1,3-propandiol: L5 (≠ F5), Q10 (= Q10)
3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
26% identity, 97% coverage: 11:400/402 of query aligns to 3:368/369 of 3pfdC
- active site: L116 (≠ Y128), S117 (= S129), T233 (≠ E244), E353 (≠ G385), R365 (≠ K397)
- binding dihydroflavine-adenine dinucleotide: Y114 (≠ Q126), L116 (≠ Y128), S117 (= S129), G122 (= G134), S123 (= S135), W147 (= W159), I148 (≠ T160), T149 (= T161), R259 (≠ T269), F262 (≠ A272), V266 (≠ L276), N269 (≠ D279), Q326 (≠ E336), L327 (≠ V337), G330 (≠ A342), I348 (≠ K380), Y352 (= Y384), T355 (= T387), Q357 (≠ E389)
8i4rA Crystal structure of acyl-coa dehydrogenase complexed with acetyl-coa from thermobifida fusca
27% identity, 97% coverage: 10:400/402 of query aligns to 11:380/381 of 8i4rA
- binding acetyl coenzyme *a: S132 (= S135), T134 (≠ L137), R180 (≠ E183), R234 (≠ T235), L237 (≠ K238), R238 (≠ Y239), L240 (= L241), D241 (≠ G242), R244 (= R245), E365 (≠ G385), G366 (= G386), R377 (≠ K397)
- binding flavin-adenine dinucleotide: Y123 (≠ Q126), L125 (≠ Y128), S126 (= S129), G131 (= G134), S132 (= S135), W156 (= W159), I157 (≠ T160), T158 (= T161), I360 (≠ K380), T367 (= T387), Q369 (≠ E389)
8i4pA Crystal structure of acyl-coa dehydrogenase from thermobifida fusca
27% identity, 97% coverage: 10:400/402 of query aligns to 11:380/381 of 8i4pA
- binding flavin-adenine dinucleotide: Y123 (≠ Q126), L125 (≠ Y128), S126 (= S129), G131 (= G134), S132 (= S135), W156 (= W159), I157 (≠ T160), T158 (= T161), I360 (≠ K380), Y364 (= Y384), T367 (= T387), Q369 (≠ E389)
P26440 Isovaleryl-CoA dehydrogenase, mitochondrial; IVD; Butyryl-CoA dehydrogenase; EC 1.3.8.4; EC 1.3.8.1 from Homo sapiens (Human) (see 5 papers)
27% identity, 71% coverage: 59:343/402 of query aligns to 96:387/426 of P26440
- 165:174 (vs. 126:135, 50% identical) binding
- S174 (= S135) binding
- WIT 198:200 (≠ WTT 159:161) binding
- SR 222:223 (≠ QE 182:183) binding
- G250 (= G208) to A: in IVA; uncertain significance
- Y277 (≠ T235) binding
- DLER 284:287 (≠ GHER 242:245) binding
- E286 (= E244) active site, Proton acceptor; mutation to D: Residual isovaleryl-CoA dehydrogenase activity.; mutation to G: Loss of isovaleryl-CoA dehydrogenase activity. Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 407-E.; mutation to Q: Loss of isovaleryl-CoA dehydrogenase activity.
- A291 (= A249) to V: in IVA; uncertain significance; dbSNP:rs886042098
- R312 (≠ T269) binding
- Q323 (≠ V280) binding
- I379 (≠ M335) to T: in IVA; uncertain significance
- QCFGG 380:384 (≠ EVAGP 336:340) binding
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
- 398 R → Q: in IVA; uncertain significance; dbSNP:rs1477527791
- 403 Y → N: in IVA; uncertain significance
- 407 A→E: Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 286-D.
- 407:408 binding
- 409:411 binding
Query Sequence
>RR42_RS36645 FitnessBrowser__Cup4G11:RR42_RS36645
MDLRFSAAEQAFREEVRSFVQASLPADIRDKVLAHQRVEKDDYVRWHRILHARGWGAPTW
PKEWGGTGWNALQRLIFEIEAFRAGAPRLLPFGLTMIGPVLMKYASQEHKERFLPRIPTV
EDFWCQGYSEPGSGSDLASLKTRAVRRGDRYIVNGQKTWTTMAHFADWIFCLVRTDSESK
PQEGISMLLIDMKTPGVTVRPIVTLDGGHDVNEVWFEDVEVPAGNLLGEENRGWTYAKYL
LGHERTGIAGIGHCNRELRQLKHYATQATDGAGRPLIDDVRMRDKIARIEMDIMALEMLL
LRVATQDAGRGPGPEASIVKIRGSEIQQDLAMLQMEVAGPNAWPYSPRWLEAGEAPPADA
PLHAPAWAAPAASTYFDMRKTSIYGGTTEVQKNIISKMILGF
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory