SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SM_b20069 FitnessBrowser__Smeli:SM_b20069 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q6LX42 Sodium/alanine symporter AgcS; Alanine permease; Alanine/glycine:cation symporter from Methanococcus maripaludis (strain S2 / LL) (see paper)
53% identity, 94% coverage: 9:448/467 of query aligns to 8:446/453 of Q6LX42

query
sites
Q6LX42

N

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T75 (= T77) binding ; binding
G79 (= G81) binding
N80 (= N82) binding ; mutation to A: Markedly reduces L-alanine uptake; when associated with A-274 and A-308.
I165 (= I165) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
Q170 (= Q170) binding ; binding
F273 (= F277) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
FS 273:274 (= FS 277:278) binding
FSNE 273:276 (= FSNE 277:280) binding
S274 (= S278) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-308.
D308 (= D312) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-274.
W370 (= W371) mutation to Q: Does not change uptake of L-alanine, but reduces the ability to transport D-alanine.

6csfM Crystal structure of sodium/alanine symporter agcs with d-alanine bound (see paper)
53% identity, 93% coverage: 16:448/467 of query aligns to 1:426/431 of 6csfM

query
sites
6csfM

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binding d-alanine: T58 (= T77), G60 (= G79), G62 (= G81), N63 (= N82), Q150 (= Q170), F253 (= F277), S254 (= S278)
binding sodium ion: T58 (= T77), I59 (= I78), N63 (= N82), S254 (= S278), D288 (= D312)

6cseM Crystal structure of sodium/alanine symporter agcs with l-alanine bound (see paper)
53% identity, 93% coverage: 16:448/467 of query aligns to 1:426/431 of 6cseM

query
sites
6cseM

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F

A

A

F

Y

T

S

T

T

I

V

L

L

G

G

W

W

A

S

Y

Y

Y

Y

A

G

E

E

K

K

C

C

W

F

E

E

Y

Y

L

L

I

I

G

G

T

T

A

K

S

G

A

I

I

R

P

L

F

Y

R

R

I

I

V

A

W

F

T

V

V

L

A

V

V

A

F

F

F

W

G

G

A

A

T

T

L

A

S

S

L

L

D

P

F

L

A

V

W

W

L

N

V

I

A

A

D

D

T

T

L

L

N

N

A

G

L

A

M

M

A

A

I

I

P

P

N

N

L

L

I

I

S

G

L

L

L

L

L

L

L

L

S

S

P

G

V

V

I

V

A

V

Q

S

L

E

T

T

R

K

D

A

Y

-

F

F

A

N

E

E

E

I

R

R

binding alanine: A57 (= A76), T58 (= T77), G60 (= G79), N63 (= N82), Q150 (= Q170), F253 (= F277), S254 (= S278), E256 (= E280)
binding sodium ion: T58 (= T77), I59 (= I78), N63 (= N82), S254 (= S278), D288 (= D312)

Query Sequence

>SM_b20069 FitnessBrowser__Smeli:SM_b20069
MEALNQFFNWLNGIVWGVPMIVLIIGTGLYLQLRLGFMPIRKVAYGFRMILKSRRPGRAA
EGEITPYAALMTALSATIGTGNIAGVATAIAVGGPGALFWMWVTAFVGMATKYAEVVVAV
KYREVDDKGEHAGGPMFAIKNGLGKHWQWLGTAFAIFGGLAGFGIGNMVQANGIASAVEN
AFGIETWISGIVMTVLTGAVILGGIKRIGAVAEKVVPFMAIFYLVCVAAVLVVFAGNIPQ
AIATIFTQAFSPTAATGGFLGSTVLMAIRMGVARGIFSNEAGLGTAGIAQAAGSTANPVF
SGVIGMMGTFIDTIIVCTLTGLAIMVSGVWASGETGAVLSSAAFEAALPGYGNYLVTISL
ALFAFTTILGWAYYAEKCWEYLIGTASAIPFRIVWTVAVFFGATLSLDFAWLVADTLNAL
MAIPNLISLLLLSPVIAQLTRDYFAEERAGAGVASHSQGQSASRKSV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory