SitesBLAST
Comparing SM_b20131 FitnessBrowser__Smeli:SM_b20131 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3hrdD Crystal structure of nicotinate dehydrogenase (see paper)
53% identity, 98% coverage: 1:158/161 of query aligns to 1:158/160 of 3hrdD
- binding flavin-adenine dinucleotide: E44 (≠ V43), G45 (= G44), E46 (≠ D45)
- binding fe2/s2 (inorganic) cluster: E40 (≠ K39), C42 (= C41), S43 (≠ D42), G45 (= G44), C47 (= C46), G48 (= G47), C50 (= C49), C62 (= C61), Q100 (= Q100), C101 (= C101), G102 (= G102), C104 (= C104), C136 (= C136), C138 (= C138)
- binding pterin cytosine dinucleotide: Q100 (= Q100), C138 (= C138)
Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
54% identity, 95% coverage: 1:153/161 of query aligns to 1:153/157 of Q0QLF3
- C42 (= C41) binding
- C47 (= C46) binding
- C50 (= C49) binding
- C62 (= C61) binding
- C101 (= C101) binding
- C104 (= C104) binding
- C136 (= C136) binding
- C138 (= C138) binding
4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
51% identity, 94% coverage: 5:155/161 of query aligns to 12:160/161 of 4zohC
- binding fe2/s2 (inorganic) cluster: C47 (= C41), S50 (≠ G44), C52 (= C46), G53 (= G47), C55 (= C49), C67 (= C61), C106 (= C101), G107 (= G102), C109 (= C104), C141 (= C136), C143 (= C138)
- binding pterin cytosine dinucleotide: Q105 (= Q100), C143 (= C138)
1sb3C Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
54% identity, 94% coverage: 1:152/161 of query aligns to 1:151/161 of 1sb3C
- binding fe2/s2 (inorganic) cluster: Q39 (≠ K39), C41 (= C41), G44 (= G44), C46 (= C46), G47 (= G47), C49 (= C49), C61 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C135 (= C136), C137 (= C138)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C137 (= C138)
1t3qA Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
50% identity, 95% coverage: 5:157/161 of query aligns to 6:159/162 of 1t3qA
- binding fe2/s2 (inorganic) cluster: I40 (≠ K39), C42 (= C41), E43 (≠ D42), G45 (= G44), C47 (= C46), G48 (= G47), C50 (= C49), R60 (≠ N59), C62 (= C61), C101 (= C101), G102 (= G102), C104 (= C104), C136 (= C136), C138 (= C138)
- binding pterin cytosine dinucleotide: Q100 (= Q100), C138 (= C138)
5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
50% identity, 95% coverage: 5:157/161 of query aligns to 5:157/157 of 5y6qA
- binding fe2/s2 (inorganic) cluster: G40 (= G40), C41 (= C41), D42 (= D42), G44 (= G44), C46 (= C46), G47 (= G47), C49 (= C49), C61 (= C61), C101 (= C101), G102 (= G102), C104 (= C104), C136 (= C136), C138 (= C138)
- binding pterin cytosine dinucleotide: Q100 (= Q100), C138 (= C138)
1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
51% identity, 97% coverage: 6:161/161 of query aligns to 5:162/906 of 1dgjA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K39), G39 (= G40), C40 (= C41), G41 (≠ D42), G43 (= G44), Q44 (≠ D45), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ N59), C60 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
- binding pterin cytosine dinucleotide: Q99 (= Q100), C139 (= C138)
Sites not aligning to the query:
- active site: 391, 427, 503, 507, 535, 869, 870
- binding molybdenum (iv)oxide: 424, 535, 698, 869
- binding pterin cytosine dinucleotide: 423, 424, 535, 652, 655, 656, 657, 658, 697, 698, 700, 702, 703, 799, 800, 803, 804, 807, 865, 866, 867, 868, 869
1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
46% identity, 96% coverage: 3:157/161 of query aligns to 2:155/155 of 1ffvA
- binding fe2/s2 (inorganic) cluster: I38 (≠ K39), C40 (= C41), S43 (≠ G44), C45 (= C46), G46 (= G47), C48 (= C49), C60 (= C61), C99 (= C101), G100 (= G102), C102 (= C104), C134 (= C136), C136 (= C138)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q100), C136 (= C138)
1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
46% identity, 96% coverage: 3:157/161 of query aligns to 3:156/156 of 1ffuD
- binding fe2/s2 (inorganic) cluster: C41 (= C41), S44 (≠ G44), H45 (≠ D45), C46 (= C46), G47 (= G47), C49 (= C49), C61 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C135 (= C136), C137 (= C138)
P19921 Carbon monoxide dehydrogenase small chain; CO dehydrogenase subunit S; CO-DH S; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
45% identity, 94% coverage: 5:155/161 of query aligns to 6:156/166 of P19921
- C42 (= C41) binding
- C47 (= C46) binding
- C50 (= C49) binding
- C62 (= C61) binding
- C102 (= C101) binding
- C105 (= C104) binding
- C137 (= C136) binding
- C139 (= C138) binding
1n5wD Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
45% identity, 94% coverage: 5:155/161 of query aligns to 4:154/158 of 1n5wD
- binding flavin-adenine dinucleotide: S43 (≠ G44), H44 (≠ D45)
- binding fe2/s2 (inorganic) cluster: C40 (= C41), S43 (≠ G44), C45 (= C46), G46 (= G47), C48 (= C49), C60 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C135 (= C136), C137 (= C138)
- binding pterin cytosine dinucleotide: Q99 (= Q100), C137 (= C138)
1n5wA Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
45% identity, 94% coverage: 5:155/161 of query aligns to 4:154/161 of 1n5wA
- binding flavin-adenine dinucleotide: S43 (≠ G44), H44 (≠ D45)
- binding fe2/s2 (inorganic) cluster: I38 (≠ K39), G39 (= G40), C40 (= C41), S43 (≠ G44), C45 (= C46), G46 (= G47), C48 (= C49), C60 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C135 (= C136), C137 (= C138)
4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
51% identity, 88% coverage: 7:148/161 of query aligns to 6:149/907 of 4usaA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ N59), C60 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 531, 532, 535, 539
- binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
51% identity, 88% coverage: 7:148/161 of query aligns to 6:149/907 of 4us9A
- binding fe2/s2 (inorganic) cluster: V38 (≠ K39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ N59), C60 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 3-phenylpropanal: 255, 257, 258, 752
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
51% identity, 88% coverage: 7:148/161 of query aligns to 6:149/907 of 4us8A
- binding fe2/s2 (inorganic) cluster: V38 (≠ K39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ N59), C60 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539
- binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
51% identity, 88% coverage: 7:148/161 of query aligns to 6:149/907 of 4c7yA
- binding fe2/s2 (inorganic) cluster: C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ N59), C60 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 535, 539
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
- binding hydrogen peroxide: 696, 697, 869
3fc4A Ethylene glycol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
51% identity, 88% coverage: 7:148/161 of query aligns to 6:149/907 of 3fc4A
- binding fe2/s2 (inorganic) cluster: V38 (≠ K39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ N59), C60 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 1,2-ethanediol: 535, 622, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
3fahA Glycerol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
51% identity, 88% coverage: 7:148/161 of query aligns to 6:149/907 of 3fahA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ N59), C60 (= C61), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding glycerol: 416, 535, 622, 683, 696, 697, 869, 884, 889, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
1sijA Crystal structure of the aldehyde dehydrogenase (a.K.A. Aor or mop) of desulfovibrio gigas covalently bound to [aso3]- (see paper)
51% identity, 88% coverage: 7:148/161 of query aligns to 6:149/907 of 1sijA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ N59), C60 (= C61), Q99 (= Q100), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding arsenite: 535, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 698, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
Q46509 Aldehyde oxidoreductase; Molybdenum iron sulfur protein; EC 1.2.99.7 from Megalodesulfovibrio gigas (Desulfovibrio gigas) (see paper)
51% identity, 88% coverage: 7:148/161 of query aligns to 6:149/907 of Q46509
- C40 (= C41) binding
- C45 (= C46) binding
- C48 (= C49) binding
- C60 (= C61) binding
- C100 (= C101) binding
- C103 (= C104) binding
- C137 (= C136) binding
- C139 (= C138) binding
Query Sequence
>SM_b20131 FitnessBrowser__Smeli:SM_b20131
MNRLVSMTVNGEARELAVVPNRTLLDALRNEGSLTGTKKGCDVGDCGACTVIMDGRPVNA
CLVLAIEAEGATIETIEGLQPAYDQPHLLQQKFMEHGGAQCGFCTPGIIMMAKALLDENP
DPSEDEIRFALAGNICRCTGYTKIIDAVRATAEELRKAGTL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory