SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SM_b20282 FitnessBrowser__Smeli:SM_b20282 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
24% identity, 57% coverage: 60:208/263 of query aligns to 259:427/490 of 4ki0F

query
sites
4ki0F

D

Q

K

K

Q

P

F

F

I

L

E

A

A

I

S

F

L

V

Y

W

S

T

I

V

G

V

I

F

A

S

L

L

A

I

T

T

T

V

A

F

A

L

A

T

T

V

V

A

I

V

G

G

V

M

W

V

I

L

A

A

L

C

L

L

V

V

S

Q

T

W

E

E

G

A

I

L

W

R

G

G

K

K

A

A

I

V

I

Y

F

R

A

V

L

L

A

L

C

I

L

L

P

P

A
x
Y

V

A

V

V

P

P

G

S

L

F

I

I

N

S

A

I

I

L

S

I

M

F

R

K

-

G

I

L

F

F

I

N

R

Q

T

S

V

F

-

G

-

E

-

I

-

N

-

M

-

M

-

L

-

S

-

A

-

L

-

F

-

G

-

V

-

K

-

P

-

A

-

W

-

F

-

S

D

D

I

P

P

T

T

T

G

A

T

R

A

T

A

M

I

L

I

I

L

I

S

V

H
x
N

A

T

V

W

H

L

A
x
G

V

Y

P

P

F
x
Y

V

M

V

M

I

I

M

L

V

C

L

M

T

G

R

L

L

L

R

K

S

A

M

I

P

P

A

D

N

D

L

L

V

Y

D

E

A

A

A

S

R

A

D

M

L

D

G

G

A

A

D

G

R

P

F

F

V

Q

A

N

F

F

L

F

R

K

V

I

T

T

I

L

P

P

Y

L

L

L

M

I

P

K

A

P

L

L

L

T

G

P

G

L

M

M

I

I

F

A

C
x
S

V

F

L

A

T

F

S
x
N

I

F

D

N

D

N

F

F

V

V

binding alpha-D-glucopyranose: Y310 (≠ A111), N361 (≠ H142), G365 (≠ A146), Y368 (≠ F149), S418 (≠ C199), N422 (≠ S203)

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
24% identity, 57% coverage: 60:208/263 of query aligns to 274:442/514 of P02916

query
sites
P02916

D

Q

K

K

Q

P

F

F

I

L

E

A

A

I

S

F

L

V

Y

W

S

T

I

V

G

V

I

F

A

S

L

L

A

I

T

T

T

V

A

F

A

L

A

T

T

V

V

A

I

V

G

G

V

M

W

V

I

L

A

A

L

C

L

L

V

V

S

Q

T

W

E

E

G

A

I

L

W

R

G

G

K

K

A

A

I

V

I

Y

F

R

A

V

L

L

A

L

C

I

L

L

P

P

A

Y

V

A

V

V

P

P

G

S

L

F

I

I

N

S

A

I

I
x
L

S

I

M

F

R

K

-

G

I

L

F

F

I

N

R

Q

T

S

V

F

-

G

-

E

-

I

-

N

-

M

-

M

-

L

-

S

-

A

-

L

-

F

-

G

-

V

-

K

-

P

-

A

-

W

-

F

-

S

D

D

I

P

P

T

T

T

G

A

T

R

A

T

A

M

I
x
L

I

I

L

I

S

V

H
x
N

A

T

V

W

H

L

A
x
G

V

Y

P

P

F

Y

V

M

V

M

I

I

M

L

V

C

L

M

T

G

R

L

L

L

R

K

S

A

M

I

P

P

A

D

N

D

L

L

V

Y

D
x
E

A

A

A
x
S

R

A

D

M

L

D

G
|
G

A

A

D

G

R

P

F

F

V

Q

A

N

F

F

L

F

R

K

V

I

T

T

I

L

P
|
P

Y

L

L

L

M

I

P

K

A

P

L

L

L

T

G

P

G

L

M

M

I

I

F

A

C

S

V

F

L

A

T

F

S

N

I

F

D

N

D

N

F

F

V

V

L334 (≠ I120) mutation to W: Ability to transport lactose in a saturable manner.
L372 (≠ I138) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ H142) mutation N->K,H: No growth on maltose.
G380 (≠ A146) mutation G->C,S: No growth on maltose.
E401 (≠ D167) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A169) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G173) mutation G->A,P: No effect.
P420 (= P186) mutation to A: No effect.

Query Sequence

>SM_b20282 FitnessBrowser__Smeli:SM_b20282
MRPYFADLPKTALGAAYWLFAVYLLLPLALMTAMSFKDASFVAFPITDWTLNWYAQVLND
KQFIEASLYSIGIALATTAAATVIGVWIALLVSTEGIWGKAIIFALACLPAVVPGLINAI
SMRIFIRTVDIPTGTAAIILSHAVHAVPFVVIMVLTRLRSMPANLVDAARDLGADRFVAF
LRVTIPYLMPALLGGMIFCVLTSIDDFVRTFFLGGYKPTLPMLIFAKVQGGMSPEINAMA
TIVLVVTAAVGVYAEHLTRRSRN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory