SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SM_b20313 FitnessBrowser__Smeli:SM_b20313 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 7 hits to proteins with known functional sites (download)

Q9CIV7 PTS-dependent dihydroxyacetone kinase, ADP-binding subunit DhaL; EC 2.7.1.121 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see paper)
39% identity, 82% coverage: 32:218/227 of query aligns to 16:187/192 of Q9CIV7

query
sites
Q9CIV7

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D29 (= D45) binding
D34 (= D50) binding
D36 (= D52) binding
HGAN 37:40 (≠ HGVN 53:56) binding
AS 78:79 (≠ SM 93:94) binding
R114 (≠ I134) mutation to A: Reduces activity 3-fold.; mutation to E: Reduces activity 100-fold.
G115 (= G135) binding
M124 (≠ L144) binding
R161 (= R192) mutation to A: Loss of activity.
Y164 (≠ R195) mutation to A: Reduces activity about 20-fold.
DPG 174:176 (≠ DAG 205:207) binding

3cr3A Structure of a transient complex between dha-kinase subunits dham and dhal from lactococcus lactis (see paper)
39% identity, 82% coverage: 32:218/227 of query aligns to 16:187/192 of 3cr3A

query
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3cr3A

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binding adenosine-5'-diphosphate: D29 (= D45), D34 (= D50), D36 (= D52), H37 (= H53), N40 (= N56), G77 (= G92), A78 (≠ S93), S79 (≠ M94), L82 (= L97), G115 (= G135), A117 (= A137), T123 (= T143), D174 (= D205), P175 (≠ A206), G176 (= G207)
binding magnesium ion: D29 (= D45), D34 (= D50), D34 (= D50), D36 (= D52), D36 (= D52)

P76014 PEP-dependent dihydroxyacetone kinase, ADP-binding subunit DhaL; EC 2.7.1.121 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 89% coverage: 22:223/227 of query aligns to 14:209/210 of P76014

query
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P76014

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D30 (= D45) binding
D35 (= D50) binding
D37 (= D52) binding
HGLN 38:41 (≠ HGVN 53:56) binding
AS 79:80 (≠ SM 93:94) binding
G121 (= G135) binding
M130 (≠ L144) binding
R178 (= R192) binding
DPG 191:193 (≠ DAG 205:207) binding

4lrzA Crystal structure of the e.Coli dhar(n)-dhal complex (see paper)
36% identity, 89% coverage: 22:223/227 of query aligns to 15:210/211 of 4lrzA

query
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4lrzA

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binding adenosine-5'-diphosphate: D31 (= D45), D36 (= D50), D38 (= D52), H39 (= H53), G79 (= G92), A80 (≠ S93), S81 (≠ M94), L84 (= L97), G122 (= G135), T130 (= T143), M131 (≠ L144), G178 (= G191), D192 (= D205), P193 (≠ A206), G194 (= G207)
binding magnesium ion: D31 (= D45), D36 (= D50), D36 (= D50), D38 (= D52), D38 (= D52)

Q3LXA3 Triokinase/FMN cyclase; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); EC 2.7.1.28; EC 2.7.1.29; EC 4.6.1.15 from Homo sapiens (Human) (see 4 papers)
33% identity, 74% coverage: 40:207/227 of query aligns to 391:558/575 of Q3LXA3

query
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Q3LXA3

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D401 (= D50) mutation to A: Abolishes both kinase and FMN cyclase activities.
D403 (= D52) mutation to A: Abolishes both kinase and FMN cyclase activities.
C404 (≠ H53) mutation to A: Decreases both kinase and FMN cyclase activities.
G445 (= G92) to S: in TKFCD; very severe decrease of triokinase and glycerone kinase activities; dbSNP:rs1590578831
S446 (= S93) mutation to A: Decreases both kinase and FMN cyclase activities.
R543 (= R192) to I: in TKFCD; reduced protein levels in patient cells; very severe decrease of triokinase and glycerone kinase activities; dbSNP:rs547013163
D556 (= D205) mutation to A: Abolishes both kinase and FMN cyclase activities.

Sites not aligning to the query:

112 T→A: Highly decreases kinase activity. No effect on FMN cyclase activity.
185 A → T: in dbSNP:rs2260655
204 K→A: Slightly decreases kinase activity. No effect on FMN cyclase activity.
221 H→A: Abolishes kinase activity but not FMN cyclase activity.

P45510 Dihydroxyacetone kinase; DHA kinase; Glycerone kinase; EC 2.7.1.29 from Citrobacter freundii (see 2 papers)
31% identity, 93% coverage: 10:219/227 of query aligns to 345:547/552 of P45510

query
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P45510

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Y

G
|
G

S

G

A

A

K

D

V

E

G

G

D

D

K

R

T
|
T

L
x
M

L

I

D

D

T

A

L

L

V

Q

P

P

A

A

V

L

D

T

A

S

F

L

D

-

A

-

T

L

A

A

D

Q

G

P

R

K

P

N

F

L

A

Q

E

A

A

A

L

F

D

D

A

A

L

A

V

Q

S

A

A

G

A

A

E

E

A

R

G

T

R

C

D

L

S

S

T

S

R

K

D

-

L

-

I

-

A

A

R

N

I

A

G

G

R

R

A

A

S

S

R

Y

L

L

G

S

E

S

R

E

S

S

-

L

L

L

G

G

V

N

L

M

D
|
D

A
x
P

G
|
G

A

A

T

Q

S

R

C

L

A

A

I

M

I

V

L

F

R

K

E

A

L

L

S

A

D380 (= D45) mutation to A: Loss of kinase activity.
D385 (= D50) mutation to A: Loss of kinase activity.
DGDT 385:388 (≠ DGDH 50:53) binding
D387 (= D52) mutation to A: Loss of kinase activity.
T388 (≠ H53) mutation to H: Reduced kinase activity.
SS 431:432 (≠ SM 93:94) binding
G468 (= G135) binding
TM 476:477 (≠ TL 143:144) binding
DPG 533:535 (≠ DAG 205:207) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
220 active site, Tele-hemiaminal-histidine intermediate

1un9A Crystal structure of the dihydroxyacetone kinase from c. Freundii in complex with amp-pnp and mg2+ (see paper)
29% identity, 93% coverage: 10:219/227 of query aligns to 345:534/537 of 1un9A

query
sites
1un9A

S

S

E

A

E

R

M

V

D

E

M

F

Q

Q

T

P

F

S

E

A

N

N

A

A

R

L

A

V

G

A

D

G

I

I

V

V

L

E

T

L

L

V

A

T

D

A

R

T

I

L

I

S

E

D

N

L

R

E

A

T

Y

H

L

L

S

N

E

A

I

L

D
|
D

G

A

K

K

I

V

G

G

D
|
D

G

G

D
|
D

H
x
T

G

G

V

S

N
x
T

M

F

A

A

K

A

G

A

F

A

G

R

L

E

A

I

A

A

E

S

R

L

L

L

K

H

G

R

K

Q

D

Q

-

L

-

P

-

L

A

N

S

N

L

L

A

A

E

T

A

L

L

F

D

A

T

L

L

I

G

G

T

E

V

R

L

L

M

T

T

V

E

V

I

M

G

G

G
|
G

S
|
S

M
x
S

G

G

P

V

L
|
L

Y

M

G

S

V

I

M

F

F

F

T

T

E

A

F

A

A

G

E

Q

K

K

I

L

E

E

-

Q

G

G

A

A

D

N

A

V

I

V

D

E

A

A

A

-

Y

-

S

-

R

-

M

-

L

L

H

N

A

T

G

G

L

L

E

A

G

Q

I

M

R

K

S

F

I

Y

G
|
G

S

G

A
|
A

K

D

V

E

G

G

D

D

K

R

T
|
T

L
x
M

L

I

D

D

T

A

L

L

V

Q

P

P

A

A

V

L

D

T

A

S

F

L

D

L

A

A

A

Q

T

P

A

K

D

N

G

-

R

-

P

-

F

-

A

-

E

-

A

-

L

L

D

Q

A

A

L

A

V

F

S

D

A

A

E

Q

A

A

G

G

R

A

D

E

S

R

T

T

R

-

D

-

L

-

I

-

A

-

R

-

I

-

G

-

R

-

A

-

S

-

R

C

L

L

G

S

E

S

R

K

S

A

L

L

G

G

V

N

L

M

D
|
D

A

P

G
|
G

A

A

T

Q

S

A

C

L

A

A

I

M

I

V

L

F

R

K

E

A

L

L

S

A

binding phosphoaminophosphonic acid-adenylate ester: D385 (= D50), D387 (= D52), T388 (≠ H53), T391 (≠ N56), G430 (= G92), S431 (= S93), S432 (≠ M94), L435 (= L97), G468 (= G135), A470 (= A137), T476 (= T143), M477 (≠ L144), D520 (= D205), G522 (= G207)
binding magnesium ion: D380 (= D45), D385 (= D50), D385 (= D50), D387 (= D52), D387 (= D52)

Sites not aligning to the query:

binding Dihydroxyacetone: 57, 61, 83, 109, 114, 220

Query Sequence

>SM_b20313 FitnessBrowser__Smeli:SM_b20313
MSRSAAPPFSEEMDMQTFENARAGDIVLTLADRIIENRAYLSEIDGKIGDGDHGVNMAKG
FGLAAERLKGKDASLAEALDTLGTVLMTEIGGSMGPLYGVMFTEFAEKIEGADAIDAAAY
SRMLHAGLEGIRSIGSAKVGDKTLLDTLVPAVDAFDAATADGRPFAEALDALVSAAEAGR
DSTRDLIARIGRASRLGERSLGVLDAGATSCAIILRELSHGARARLA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory