SitesBLAST

P54582 Glycine betaine transporter BetP; Glycine betaine permease from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see 6 papers)
40% identity, 64% coverage: 88:541/706 of query aligns to 93:548/595 of P54582

query
sites
P54582

T

S

A

A

I

V

L

V

S

D

G

N

F

L

G

G

W
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W

L

A

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F

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G

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G

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G

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M

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x
F

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G

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T

G

T

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E

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M

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H

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G

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-

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V

W101 (= W96) mutation to A: Mainly monomeric, shows a decrease in activity and cannot be activated in response to increased osmolality; when associated with A-351.
E135 (= E130) mutation to A: Strongly decreased betaine transport.
G149 (= G144) mutation to A: Decreases betaine transport. No effect on activation by increased osmolality.
M150 (= M145) mutation to F: No effect on activation by increased osmolality; when associated with A-152.
G151 (= G146) mutation to A: Nearly abolishes betaine transport.
I152 (= I147) mutation to A: No effect on activation by increased osmolality; when associated with F-150.
IG 152:153 (= IG 147:148) binding glycine betaine
G153 (= G148) mutation to A: Decreases betaine transport and alters activation at higher osmolality.; mutation to D: Changes substrate specificity, giving rise to proton-coupled choline transport. Decreases sodium-dependent betaine transport.
F156 (≠ Y151) mutation to A: Decreases betaine transport, but has no major effect on affinity for glycine betaine.
W189 (= W186) mutation to C: Mildly decreased betaine transport.
W194 (= W191) mutation to L: Strongly decreased betaine transport.
Y197 (= Y194) mutation to L: Nearly abolishes betaine transport.
R210 (= R207) mutation to A: Nearly abolishes betaine transport.
S253 (= S249) binding glycine betaine
G301 (= G296) mutation to L: Strongly decreased betaine transport.
N309 (= N304) mutation to A: Decreases affinity for sodium ions.
T351 (= T343) mutation to A: Mainly trimeric, but shows reduced activity at high osmolalities. Mainly monomeric, shows a decrease in activity and cannot be activated in response to increased osmolality; when associated with A-101.
W362 (= W354) mutation to C: Strongly decreased betaine transport.
W366 (= W358) mutation to C: No effect on betaine transport.
F369 (= F361) mutation to G: Decreases affinity for glycine betaine. Decreases betaine transport.
W371 (= W363) mutation to L: No effect on betaine transport.
W373 (= W365) mutation to A: Strongly decreases affinity for glycine betaine and betaine transport.
WWISW 373:377 (= WWISW 365:369) binding glycine betaine
W374 (= W366) mutation to A: Strongly decreases betaine transport, but has no major effect on affinity for glycine betaine.; mutation to L: No effect on betaine transport.
W377 (= W369) mutation to A: Abolishes betaine transport.; mutation to L: Nearly abolishes betaine transport.
F380 (= F372) mutation to A: Decreases betaine transport, but has no effect on affinity for glycine betaine.
F384 (= F376) mutation to A: Decreases betaine transport, but has no effect on affinity for glycine betaine.
R387 (= R379) mutation to A: Mildly decreased betaine transport.
R392 (= R384) mutation to K: Moderately decreased betaine transport.

4llhA Substrate bound outward-open state of the symporter betp (see paper)
40% identity, 64% coverage: 88:541/706 of query aligns to 37:489/524 of 4llhA

query
sites
4llhA

T

S

A

A

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K

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binding 2-(trimethyl-lambda~5~-arsanyl)ethanol: M94 (= M145), G95 (= G146), D97 (≠ G148), W314 (= W365), W315 (= W366), W318 (= W369)
binding sodium ion: A91 (= A142), M94 (= M145), G95 (= G146), F405 (= F460), T408 (= T463), S409 (= S464)

Sites not aligning to the query:

binding 5-cyclohexyl-1-pentyl-beta-d-maltoside: 495, 499

3p03C Crystal structure of betp-g153d with choline bound (see paper)
40% identity, 64% coverage: 88:541/706 of query aligns to 37:488/508 of 3p03C

query
sites
3p03C

T

S

A

A

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L

V

S

D

G

N

F

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G

G

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W

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A

Y

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W

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M

L

M

F

F

A

A

A
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A

G

G

M
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M

G

G

I

I

G
x
D

L

L

M

M

Y

F

F

Y

A

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T

G

T

E

E

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P

M

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F

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P

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P

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A

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A

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M

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M

F

F

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H

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A

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G

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A

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Y

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L

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K

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A

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A

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L

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K

K

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N

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A

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A

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S

I

I

G

G

L

N

Y

Y

L

L

D

S

S

N

-

F

-

Q

L

M

V

A

L

G

R

R

T

T

F

A

N

M

I

S

Y

A

A

D

Y

G

E

T

P

A

R

G

P

E

W

W

I

L

D

G

S

S

W

W

T

T

L

I

F

F

Y

Y

W

W

A

A

W

W

I

I

S

S

W

W

S

S

P

P

F
|
F

V

V

G

G

M

M

F

F

I

L

A

A

R

R

I

I

S

S

R

R

G

G

R

R

T

S

V

I

R

R

E

E

F

F

V

I

T

L

A

G

V

V

L

L

F

L

V

V

P

P

A

A

M

G

F

V

T

S

F

T

L

V

W

W

M

F

T

S

V

I

F

F

G

G

N

G

T

T

A

A

I

I

Y

V

V

F

D

E

T

Q

T

-

I

-

A

-

N

N

G

G

E

E

-

S

L

I

A

W

R

G

D

D

V

G

K

A

A

A

D

E

L

E

S

Q

V

-

A

-

L

L

F

F

Q

G

F

L

E

H

Y

A

L

L

P

P

W

G

P

G

A

Q

V

I

T

M

S

G

T

I

L

I

A

A

V

M

L

I

L

L

V

L

S

G

I

T

F

F

V

I

T

T

S

S

S

A

D

D

S

S

G

A

S

S

L

T

V

V

I

M

D

G

T

T

I

M

A

S

S

Q

G

H

G

G

E

Q

T

L

A

E

T

A

P

N

A

K

L

W

Q

V

R

T

I

A

F

A

W

W

C

G

S

V

L

A

S

T

G

A

I

A

V

I

A

G

A

L

V

T

L

L

S

L

T

S

G

G

-

D

-

N

-

A

L

L

T

S

A

N

L

L

Q

Q

S

N

A

V

T

T

I

I

S

V

T

A

A

A

L

T

P

P

F

F

S

L

L

F

V

V

M

V

L

I

I

G

L

L

V

M

W

F

S

A

L

L

F

V

V

K

G

D

M

L

R

S

A

N

D

D

L

V

binding choline ion: A92 (= A143), M94 (= M145), D97 (≠ G148), F320 (= F372)

P31553 L-carnitine/gamma-butyrobetaine antiporter from Escherichia coli (strain K12) (see 3 papers)
28% identity, 69% coverage: 46:529/706 of query aligns to 3:490/504 of P31553

query
sites
P31553

H

N

Q

E

V

K

Q

R

R

K

F

T

K

G

V

I

N

E

L

P

P

K

V

V

F

F

V

F

G

P

S

P

V

L

A

I

V

I

I

V

A

G

L

I

F

L

V

C

G

W

I

L

G

T

V

V

I

R

A

D

P

L

K

D

R

A

A

A

E

N

S

V

I

V

F

I

S

N

G

A

M

V

Q

F

T

S

A

Y

I

V

L

T

S

N

G

V

F

W

G

G

W

W

L

A

Y

F

L

E

L

W

S

Y

V

M

A

V

V

V

F

M

L

L

F

F

S

G

M

W

L

F

F

W

L

L

A

V

F

F

S

G

R

P

Y

Y

G

A

E

K

L

K

K

R

L

L

G

G

P

-

D

-

D

N

S

E

E

P

P

P

E

E

F

F

R

S

Y

T

L

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

Y

F

F

W

A

G

V

S

G

I

E

E

P

I

M

Y

T
x
Y

H

Y

F

I

A

S

S

T

P

P

E

F

A

G

-

L

E

E

P

P

L

N

T

S

I

T

A

G

A

A

Q

K

R

E

E

L

A

G

M

L

S

A

V

Y

T

S

F

L

F

F

H

H

W
|
W

G

G

V

P

H

L

A

P

W

W

A

A

I

T

Y

Y

S

S

V

F

V

L

G

S

L

V

S

A

L

F

A

A

Y

Y

F

F

G

F

Y

F

R

V

Y

R

N

K

L

M

P

E

-

V

L

I

T

R

V

P

R

S

S

S

G

T

L

L

Y

V

P

P

L

L

L

V

K

G

E

E

G

K

-

H

I

A

H

K

G

G

P

L

I

F

G

G

H

T

V

I

V

V

D

D

I

N

F

F

A

Y

I

L

C

V

G

A

T

L

M

I

F

F

G

A

L

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

I

T

L

P

Q

L

I

V

N

T

S

E

G

C

L

M

N

Q

Y

W

L

L

L

F

G

G

I

I

P

P

Q

H

S

T

I

L

Y

Q

V

L

Q

D

L

A

L

I

V

I

T

T

V

C

V

W

T

I

A

I

I

L

A

N

T

A

I

I

S

C

V

V

V

A

T

C

G

G

V

L

E

Q

K

K

G

G

V

V

R

R

I

I

L

A

S

S

E

D

T

V

N

R

L

S

F

Y

L

L

A

S

V

F

L

L

L

M

M

L

L

G

F

W

V

V

L

F

V

I

V

V

G

S

P

G

T

A

G

S

T

F

L

I

M

M

R

N

D

Y

F

F

V

T

Q
x
D

N

S

I

V

G

G

L

M

Y

L

L

L

D
x
M

S

Y

L

L

V

P

L
x
R

R

M

T

L

F

F

N

Y

I
x
T

Y

D

A

P

Y

I

E

A

P

K

R

G

P

G

W

F

I

P

D

Q

S
x
G

W
|
W

T

T

L

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W
x
Y

S
x
A

P
x
I

F
x
Q

V
x
M

G

S

M

I

F

F

I

L

A

A

R

R

I

I

S

S

R

R

G

G

R

R

T

T

V

V

R

R

E

E

F

L

V

C

T

F

A

G

-

M

V

V

L

L

F

G

V

L

P

T

A

A

M

S

F

T

T

W

F

I

L

L

W

W

M

T

T

V

V

L

F

G

G

S

N

N

T

T

A

L

I

L

Y

L

V

I

D

D

T

K

T

N

I

I

A

I

N

N

G

-

E

-

L

-

A

I

R

P

D

N

V

L

K

I

A

E

D

Q

L

Y

S

G

V

V

A

A

L

R

F

A

Q

I

F

I

F

E

E

T

Y

W

L

A

P

A

W

L

P

P

A

L

V

S

T

T

S

A

T

T

L

M

A

W

V

G

L

F

V

I

S

L

I

C

F

F

F

I

V

A

T

T

-

V

-

T

-

L

-

V

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

T

T

I

C

-

R

-

E

A

V

S

R

G

D

G

G

E

E

T

E

A

P

T

-

P

P

A

L

L

L

Q

V

R

R

I

I

F

G

W

W

C

S

S

I

L

L

S

V

G

G

I

I

V

I

A

G

A

I

V

V

L

L

S

A

T

L

G

G

L

L

T

K

A

P

L

I

Q

Q

S

T

A

A

T

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

S

F

L

F

V

V

M

N

L

I

I

M

L

V

Y114 (≠ T159) binding 4-(trimethylamino)butanoate; mutation to L: Small decrease in transport activity.
W142 (= W186) binding (R)-carnitine
D288 (≠ Q330) mutation to A: Retains 70% of transport activity. Forms mostly monomers.; mutation to R: Abolishes transport activity. Forms mostly monomers.; mutation to W: Retains 4% of transport activity. Forms mostly monomers.
M295 (≠ D337) mutation to E: Does not affect transport activity. Forms mostly monomers. Can also form small amounts of homodimers and homotrimers.
R299 (≠ L341) mutation to A: Does not affect transport activity. Forms mostly monomers. Can also form small amounts of homodimers and homotrimers. Shows a high tendency to aggregate.
T304 (≠ I346) mutation to A: Does not affect transport activity. Forms mostly monomers. Shows a high tendency to aggregate.
GW 315:316 (≠ SW 357:358) binding 4-(trimethylamino)butanoate
W316 (= W358) mutation to L: Decrease in transport activity.
W323 (= W365) binding 4-(trimethylamino)butanoate; mutation to L: Abolishes transport activity.
WW 323:324 (= WW 365:366) binding (R)-carnitine
W324 (= W366) mutation to L: Abolishes transport activity.
Y327 (≠ W369) mutation to L: Strong decrease in transport activity.
YAIQ 327:330 (≠ WSPF 369:372) binding (R)-carnitine
Q330 (≠ F372) mutation to L: Decrease in transport activity.
M331 (≠ V373) binding 4-(trimethylamino)butanoate

3hfxA Crystal structure of carnitine transporter (see paper)
29% identity, 67% coverage: 57:529/706 of query aligns to 3:479/493 of 3hfxA

query
sites
3hfxA

V

V

F

F

V

F

G

P

S

P

V

L

A

I

V

I

I

V

A

G

L

I

F

L

V

C

G

W

I

L

G

T

V

V

I

R

A

D

P

L

K

D

R

A

A

A

E

N

S

V

I

V

F

I

S

N

G

A

M

V

Q

F

T

S

A

Y

I

V

L

T

S

N

G

V

F

W

G

G

W

W

L

A

Y

F

L

E

L

W

S

Y

V

M

A

V

V

V

F

M

L

L

F

F

S

G

M

W

L

F

F

W

L

L

A

V

F

F

S

G

R

P

Y

Y

G

A

E

K

L

K

K

R

L

L

G

G

P

-

D

-

D

N

S

E

E

P

P

P

E

E

F

F

R

S

Y

T

L

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

Y

F

F

W

A

G

V

S

G

I

E

E

P

I

M

Y

T
x
Y

H

Y

F

I

A

S

S

T

P

P

E

F

A

G

-

L

E

E

P

P

L

N

T

S

I

T

A

G

A

A

Q

K

R

E

E

L

A

G

M

L

S

A

V

Y

T

S

F

L

F

F

H

H

W
|
W

G

G

V

P

H

L

A

P

W

W

A

A

I

T

Y

Y

S

S

V

F

V

L

G

S

L

V

S

A

L

F

A

A

Y

Y

F

F

G

F

Y

F

R

V

Y

R

N

K

L

M

P

E

-

V

L

I

T

R

V

P

R

S

S

S

G

T

L

L

Y

V

P

P

L

L

L

V

K

G

E

E

G

K

-

H

I

A

H

K

G

G

P

L

I

F

G

G

H

T

V

I

V

V

D

D

I

N

F

F

A

Y

I

L

C

V

G

A

T

L

M

I

F

F

G

A

L

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

I

T

L

P

Q

L

I

V

N

T

S

E

G

C

L

M

N

Q

Y

W

L

L

L

F

G

G

I

I

P

P

Q

H

S

T

I

L

Y

Q

V

L

Q

D

L

A

L

I

V

I

T

T

V

C

V

W

T

I

A

I

I

L

A

N

T

A

I

I

S

C

V

V

V

A

T

C

G

G

V

L

E

Q

K

K

G

G

V

V

R

R

I

I

L

A

S

S

E

D

T

V

N

R

L

S

F

Y

L

L

A

S

V

F

L

L

L

M

M

L

L

G

F

W

V

V

L

F

V

I

V

V

G

S

P

G

T

A

G

S

T

F

L

I

M

M

R

N

D

Y

F

F

V

T

Q

D

N

S

I

V

G

G

L

M

Y

L

L

L

D

M

S

Y

L

L

V

P

L

R

R

M

T

L

F

F

N

Y

I

T

Y

D

A

P

Y

I

E

A

P

K

R

G

P

G

W

F

I

P

D

Q

S

G

W
|
W

T

T

L

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W
x
Y

S

A

P

I

F
x
Q

V

M

G

S

M

I

F
|
F

I

L

A

A

R

R

I

I

S

S

R

R

G

G

R

R

T

T

V

V

R

R

E

E

F

L

V

C

T

F

A

G

-

M

V

V

L

L

F

G

V

L

P

T

A

A

M

S

F

T

T

W

F

I

L

L

W

W

M

T

T

V

V

L

F

G

G

S

N

N

T

T

A

L

I

L

Y

L

V

I

D

D

T

K

T

N

I

I

A

I

N

N

G

-

E

-

L

-

A

I

R

P

D

N

V

L

K

I

A

E

D

Q

L

Y

S

G

V

V

A

A

L

R

F

A

Q

I

F

I

F

E

E

T

Y

W

L

A

P

A

W

L

P

P

A

L

V

S

T

T

S

A

T

T

L

M

A

W

V

G

L

F

V

I

S

L

I

C

F

F

F

I

V

A

T

T

-

V

-

T

-

L

-

V

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

T

T

I

C

-

R

-

E

A

V

S

R

G

D

G

G

E

E

T

E

A

P

T

-

P

P

A

L

L

L

Q

V

R

R

I

I

F

G

W

W

C

S

S

I

L

L

S

V

G

G

I

I

V

I

A

G

A

I

V

V

L

L

S

A

T

L

G

G

L

L

T

K

A

P

L

I

Q

Q

S

T

A

A

T

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

S

F

L

F

V

V

M

N

L

I

I

M

L

V

binding carnitine: Y103 (≠ T159), W131 (= W186), W305 (= W358), W312 (= W365), W313 (= W366), Y316 (≠ W369), Y316 (≠ W369), Q319 (≠ F372), F323 (= F376)

2wsxA Crystal structure of carnitine transporter from escherichia coli (see paper)
29% identity, 67% coverage: 57:529/706 of query aligns to 7:483/496 of 2wsxA

query
sites
2wsxA

V

V

F

F

V

F

G

P

S

P

V

L

A

I

V

I

I

V

A

G

L

I

F

L

V

C

G

W

I

L

G

T

V

V

I

R

A

D

P

L

K

D

R

A

A

A

E

N

S

V

I

V

F

I

S

N

G

A

M

V

Q

F

T

S

A

Y

I

V

L

T

S

N

G

V

F

W

G

G

W

W

L

A

Y

F

L

E

L

W

S

Y

V

M

A

V

V

V

F

M

L

L

F

F

S

G

M

W

L

F

F

W

L

L

A

V

F

F

S

G

R

P

Y

Y

G

A

E

K

L

K

K

R

L

L

G

G

P

-

D

-

D

E

S

E

E

P

P

P

E

E

F

F

R

S

Y

T

L

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

Y

F

F

W

A

G

V

S

G

I

E

E

P

I

M

Y

T

Y

H

Y

F

I

A

S

S

T

P

P

E

F

A

G

-

L

E

E

P

P

L

N

T

S

I

T

A

G

A

A

Q

K

R

E

E

L

A

G

M

L

S

A

V

Y

T

S

F

L

F

F

H

H

W
|
W

G

G

V

P

H

L

A

P

W

W

A

A

I

T

Y

Y

S

S

V

F

V

L

G

S

L

V

S

A

L

F

A

A

Y

Y

F

F

G

F

Y

F

R

V

Y

R

N

K

L

M

P

E

-

V

L

I

T

R

V

P

R

S

S

S

G

T

L

L

Y

V

P

P

L

L

L

V

K

G

E

E

G

K

-

H

I

A

H

K

G

G

P

L

I

F

G

G

H

T

V

I

V

V

D

D

I

N

F

F

A

Y

I

L

C

V

G

A

T

L

M

I

F

F

G

A

L

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

I

T

L

P

Q

L

I

V

N

T

S

E

G

C

L

M

N

Q

Y

W

L

L

L

F

G

G

I

I

P

P

Q

H

S

T

I

L

Y

Q

V

L

Q

D

L

A

L

I

V

I

T

T

V

C

V

W

T

I

A

I

I

L

A

N

T

A

I

I

S

C

V

V

V

A

T

C

G

G

V

L

E

Q

K

K

G

A

V

V

R

R

I

I

L

A

S

S

E

D

T

V

N

R

L

S

F

Y

L

L

A

S

V

F

L

L

L

M

M

L

L

G

F

W

V

V

L

F

V

I

V

V

G

S

P

G

T

A

G

S

T

F

L

I

M

M

R

N

D

Y

F

F

V

T

Q

D

N

S

I

V

G

G

L

M

Y

L

L

L

D

M

S

Y

L

L

V

P

L

R

R

M

T

L

F

F

N

Y

I

T

Y

D

A

P

Y

I

E

A

P

K

R

G

P
x
G

W

F

I

P

D

Q

S
x
G

W

W

T

T

L

V

F

F

Y

Y

W

W

A

A

W
|
W

W

W

I

V

S

I

W
x
Y

S

A

P

I

F

Q

V
x
M

G

S

M

I

F

F

I

L

A

A

R

R

I

I

S

S

R

R

G

G

R

R

T

T

V

V

R

R

E

E

F

L

V

C

T

F

A

G

-

M

V

V

L

L

F

G

V

L

P

T

A

A

M

S

F

T

T

W

F

I

L

L

W

W

M

T

T

V

V

L

F

G

G

S

N

N

T

T

A

L

I

L

Y

L

V

I

D

D

T

K

T

N

I

I

A

L

N

N

G

-

E

-

L

-

A

I

R

P

D

N

V

L

K

I

A

E

D

Q

L

Y

S

G

V

V

A

A

L

R

F

A

Q

I

F

I

F

E

E

T

Y

W

L

A

P

A

W

L

P

P

A

L

V

S

T

T

S

A

T

T

L

M

A

W

V

G

L

F

V

I

S

L

I

C

F

F

F

I

V

A

T

T

-

V

-

T

-

L

-

V

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

T

T

I

C

-

R

-

E

A

V

S

R

G

D

G

G

E

E

T

D

A

P

T

-

P

P

A

L

L

L

Q

V

R

R

I

I

F

G

W

W

C

S

S

I

L

L

S

V

G

G

I

I

V

I

A

G

A

I

V

V

L

L

S

A

T

L

G

G

L

L

T

K

A

P

L

I

Q
|
Q

S

T

A

A

T

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

S

F

L

F

V

V

M

N

L

I

I

M

L

V

binding 3-carboxy-n,n,n-trimethylpropan-1-aminium: W135 (= W186), G304 (≠ P353), G308 (≠ S357), W316 (= W365), Y320 (≠ W369), M324 (≠ V373), Q466 (= Q512)

2wswA Crystal structure of carnitine transporter from proteus mirabilis (see paper)
27% identity, 69% coverage: 44:529/706 of query aligns to 6:495/508 of 2wswA

query
sites
2wswA

R

H

L

M

H

K

Q

D

V

N

Q

K

R

K

F

A

K

G

V

I

N

E

L

P

P

K

V

V

F

F

V

F

G

P

S

P

V

L

A

I

V

I

I

V

A

G

L

I

F

L

V

C

G

W

I

L

G

T

V

V

I

R

A

D

P

L

K

D

R

A

A

S

E

N

S

E

I

V

F

I

S

N

G

A

M

V

Q

F

T

S

A

Y

I

V

L

T

S

N

G

V

F

W

G

G

W

W

L

A

Y

F

L

E

L

W

S

Y

V

M

A

V

V

I

F

M

L

F

F

G

S

G

M

W

L
x
F

F

W

L

L

A

V

F
|
F

S

G

R
|
R

Y

Y

G

A

E

K

L

K

K

R

L

L

G

G

P

-

D

-

D

D

S

E

E

K

P

P

E

E

F

F

R

S

Y

T

L

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

Y

F

F

W

A

G

V

S

G

I

E

E

P

I

M

Y

T

Y

H

Y

F

I

A

S

S

S

P

P

-

F

E

G

A

M

E

E

P

G

L

Y

T

S

I

A

A

P

A

A

Q

K

R

E

E

I

A

G

M

L

S

A

V

Y

T

S

F

L

F

F

H

H

W

W

G

G

V

P

H

L

A

P

W

W

A

A

I

T

Y

Y

S

S

V

F

V

L

G

S

L

V

S

A

L

F

A

A

Y

Y

F

F

G

F

Y

F

R

V

Y

R

N

K

L

M

P

E

-

V

L

I

T

R

V

P

R

S

S

S

G

T

L

L

Y

V

P

P

L

L

L

V

K

G

E

E

G

K

-

H

I

V

H

N

G

G

P

L

I

F

G

G

H

T

V

V

D

D

I

N

F

F

A

Y

I

L

C

V

G

A

T

L

M

I

F

L

G

A

L

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

I

T

L

P

Q

L

I

V

N

T

S

E

G

C

L

I

N

Q

Y

Y

L

L

L

F

G

G

I

I

P

P

Q

H

S

T

I

L

Y

Q

V

L

Q

D

L

A

L

I

V

I

T

S

V

C

V

W

T

I

A

L

I

L

A

N

T

A

I

I

S

C

V

V

V

A

T

F

G

G

V

L

E

Q

K

K

G

G

V

V

R

K

I

I

L

A

S

S

E

D

T

V

N

R

L

T

F

Y

L

L

A

S

V

F

L

L

L

M

M

L

L

G

F

W

V

V

L

F

V

I

V

V

G

G

P

G

T

A

G

S

T

F

L

I

M

V

R

N

D

Y

F

F

V

T

Q

D

N

S

I

V

G

G

L

T

Y

L

L

L

D

M

S

Y

L

M

V

P

L

R

R

M

T

L

F

F

N

Y

I

T

Y

D

A

P

Y

I

E

G

P

K

R

G

P

G

W

F

I

P

D

Q

S

A

W

W

T

T

L

V

F

F

Y

Y

W

W

A

A

W

W

I

V

S

I

W

Y

S

A

P

I

F

Q

V

M

G

S

M

I

F

F

I

L

A

A

R

R

I

I

S

S

R

K

G

G

R

R

T

T

V

V

R

R

E

E

F

L

V

C

T

L

A

G

V

M

L

V

F

S

V

G

P

L

A

T

M

A

F

G

T

T

F

W

L

L

-

I

W

W

M

T

T

I

V

L

F

G

G

G

N

N

T

T

A

L

I

Q

Y

L

V

I

D

D

T

Q

T

N

I

I

A

L

N

N

G

I

E

P

L

Q

A

L

R

I

D

D

V

-

K

Q

A

Y

D

G

L

V

S

P

V

R

A

A

L

I

F

I

Q

E

F

T

F

W

E

A

Y

A

L

L

P

-

W

-

P

P

A

L

V

S

T

T

S

A

T

T

L

M

A

W

V

G

L

F

V

I

S

L

I

C

F

F

F

I

V

A

T

T

-

V

-

T

-

L

-

I

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

T

T

I

C

A

R

S

S

G

M

G

K

E

E

T

G

A

A

T

D

P

P

A

P

L

L

Q

L

-

V

R

R

I

I

F

G

W

W

C

S

S

V

L

L

S

V

G

G

I

I

V

I

A

G

A

I

V

I

L

L

S

A

T

L

G

G

L

L

T

K

A

P

L

I

Q

Q

S

T

A

A

T

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

S

F

L

F

V

V

M

N

L

I

I

M

L

V

binding 5-cyclohexyl-1-pentyl-beta-d-maltoside: F72 (≠ L110), F76 (= F114), R78 (= R116)

B4EY22 L-carnitine/gamma-butyrobetaine antiporter from Proteus mirabilis (strain HI4320) (see 2 papers)
28% identity, 68% coverage: 49:529/706 of query aligns to 6:490/514 of B4EY22

query
sites
B4EY22

Q

K

R

K

F

A

K

G

V

I

N

E

L

P

P

K

V

V

F

F

V

F

G

P

S

P

V

L

A

I

V

I

I

V

A

G

L

I

F

L

V

C

G

W

I

L

G

T

V

V

I

R

A

D

P

L

K

D

R

A

A

S

E

N

S

E

I

V

F

I

S

N

G

A

M

V

Q

F

T

S

A

Y

I

V

L

T

S

N

G

V

F

W

G

G

W

W

L

A

Y

F

L

E

L

W

S

Y

V

M

A

V

V

I

F

M

L

F

F

G

S

G

M

W

L

F

F

W

L

L

A

V

F

F

S

G

R

R

Y

Y

G

A

E

K

L

K

K

R

L

L

G

G

P

-

D

-

D

D

S

E

E

K

P

P

E

E

F

F

R

S

Y

T

L

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

Y

F

F

W

A

G

V

S

G

I

E
|
E

P

I

M

Y

T

Y

H

Y

F

I

A

S

S

S

P

P

-

F

E

G

A

M

E

E

P

G

L

Y

T

S

I

A

A

P

A

A

Q

K

R

E

E

I

A

G

M

L

S

A

V

Y

T

S

F

L

F

F

H

H

W

W

G

G

V

P

H

L

A

P

W

W

A

A

I

T

Y

Y

S

S

V

F

V

L

G

S

L

V

S

A

L

F

A

A

Y

Y

F

F

G

F

Y

F

R

V

Y

R

N

K

L

M

P

E

-

V

L

I

T

R

V

P

R

S

S

S

G

T

L

L

Y

T

P

P

L

L

L

V

K

G

E

E

G

K

-

H

I

V

H

N

G

G

P

L

I

F

G

G

H

T

V

V

D

D

I

N

F

F

A

Y

I

L

C

V

G

A

T

L

M

I

F

L

G

A

L

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

I

T

L

P

Q

L

I

V

N

T

S

E

G

C

L

I

N

Q

Y

Y

L

L

L

F

G

G

I

I

P

P

Q

H

S

T

I

L

Y

Q

V

L

Q

D

L

A

L

I

V

I

T

S

V

C

V

W

T

I

A

L

I

L

A

N

T

A

I

I

S

C

V

V

V

A

T

F

G

G

V

L

E

Q

K

K

G

G

V

V

R

K

I

I

L

A

S

S

E

D

T

V

N
x
R

L

T

F

Y

L

L

A

S

V

F

L

L

L

M

M

L

L

G

F

W

V

V

L

F

V

I

V

V

G

G

P

G

T

A

G

S

T

F

L

I

M

V

R

N

D

Y

F

F

V

T

Q

D

N

S

I

V

G

G

L

T

Y

L

L

L

D

M

S

Y

L

M

V

P

L

R

R

M

T

L

F

F

N

Y

I

T

Y

D

A

P

Y

I

E

G

P

K

R

G

P

G

W

F

I

P

D

Q

S

A

W
|
W

T

T

L

V

F

F

Y

Y

W

W

A

A

W

W

I

V

S

I

W

Y

S

A

P

I

F

Q

V
x
M

G

S

M

I

F

F

I

L

A

A

R

R

I

I

S

S

R

K

G

G

R

R

T

T

V

V

R

R

E

E

F

L

V

C

T

L

A

G

V

M

L

V

F

S

V

G

P

L

A

T

M

A

F

G

T

T

F

W

L

L

-

I

W

W

M

T

T

I

V

L

F

G

G

G

N

N

T

T

A

L

I

Q

Y

L

V

I

D

D

T

Q

T

N

I

I

A

L

N

N

G

I

E

P

L

Q

A

L

R

I

D

D

V

-

K

Q

A

Y

D

G

L

V

S

P

V

R

A

A

L

I

F

I

Q

E

F

T

F

W

E

A

Y

A

L

L

P

-

W

-

P

P

A

L

V

S

T

T

S

A

T

T

L

M

A

W

V

G

L

F

V

I

S

L

I

C

F

F

F

I

V

A

T

T

-

V

-

T

-

L

-

I

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

T

T

I

C

A

R

S

S

G

M

G

K

E

E

T

G

A

A

T

E

P

P

A

P

L

L

Q

L

-

V

R

R

I

I

F

G

W

W

C

S

S

V

L

L

S

V

G

G

I

I

V

I

A

G

A

I

V

I

L

L

S

A

T

L

G

G

L

L

T

K

A

P

L

I

Q

Q

S

T

A

A

T

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

S

F

L

F

V

V

M

N

L

I

I

M

L

V

E111 (= E156) mutation to A: Abolishes transport activity.
R262 (≠ N304) mutation R->A,E: Strong decrease in L-carnitine transport. Mutant is Na(+)-dependent for substrate binding and transport.
W316 (= W358) mutation to A: 2.5-fold decrease in Vmax.
M331 (≠ V373) mutation to V: 10-fold decrease in Vmax.

4m8jA Crystal structure of cait r262e bound to gamma-butyrobetaine (see paper)
28% identity, 67% coverage: 57:529/706 of query aligns to 6:482/495 of 4m8jA

query
sites
4m8jA

V

V

F

F

V

F

G

P

S

P

V

L

A

I

V

I

I

V

A

G

L

I

F

L

V

C

G

W

I

L

G

T

V

V

I

R

A

D

P

L

K

D

R

A

A

S

E

N

S

E

I

V

F

I

S

N

G

A

M

V

Q

F

T

S

A

Y

I

V

L

T

S

N

G

V

F

W

G

G

W

W

L

A

Y

F

L

E

L

W

S

Y

V

M

A

V

V

I

F

M

L

F

F

G

S

G

M

W

L

F

F

W

L

L

A

V

F

F

S

G

R

R

Y

Y

G

A

E

K

L

K

K

R

L

L

G

G

P

-

D

-

D

D

S

E

E

K

P

P

E

E

F

F

R

S

Y

T

L

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

Y

F

F

W

A

G

V

S

G

I

E

E

P

I

M

Y

T

Y

H

Y

F

I

A

S

S

S

P

P

-

F

E

G

A

M

E

E

P

G

L

Y

T

S

I

A

A

P

A

A

Q

K

R

E

E

I

A

G

M

L

S

A

V

Y

T

S

F

L

F

F

H

H

W

W

G

G

V

P

H

L

A

P

W
|
W

A

A

I

T

Y

Y

S

S

V

F

V

L

G

S

L

V

S

A

L

F

A

A

Y

Y

F

F

G

F

Y

F

R

V

Y

R

N

K

L

M

P

E

-

V

L

I

T

R

V

P

R

S

S

S

G

T

L

L

Y

V

P

P

L

L

L

V

K

G

E

E

G

K

-

H

I

V

H

N

G

G

P

L

I

F

G

G

H

T

V

V

D

D

I

N

F

F

A

Y

I

L

C

V

G

A

T

L

M

I

F

L

G

A

L

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

I

T

L

P

Q

L

I

V

N

T

S

E

G

C

L

I

N

Q

Y

Y

L

L

L

F

G

G

I

I

P

P

Q

H

S

T

I

L

Y

Q

V

L

Q

D

L

A

L

I

V

I

T

S

V

C

V

W

T

I

A

L

I

L

A

N

T

A

I

I

S

C

V

V

V

A

T

F

G

G

V

L

E

Q

K

K

G

G

V

V

R

K

I

I

L

A

S

S

E

D

T

V

N

E

L

T

F

Y

L

L

A

S

V

F

L

L

L

M

M

L

L

G

F

W

V

V

L

F

V

I

V

V

G

G

P

G

T

A

G

S

T

F

L

I

M

V

R

N

D

Y

F

F

V

T

Q

D

N

S

I

V

G

G

L

T

Y

L

L

L

D

M

S

Y

L

M

V

P

L

R

R

M

T

L

F

F

N

Y

I

T

Y

D

A

P

Y

I

E

G

P

K

R

G

P

G

W

F

I

P

D

Q

S

A

W

W

T

T

L

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W

Y

S

A

P

I

F

Q

V

M

G

S

M

I

F

F

I

L

A

A

R

R

I

I

S

S

R

K

G

G

R

R

T

T

V

V

R

R

E

E

F

L

V

C

T

L

A

G

V

M

L

V

F

S

V

G

P

L

A

T

M

A

F

G

T

T

F

W

L

L

-

I

W

W

M

T

T

I

V

L

F

G

G

G

N

N

T

T

A

L

I

Q

Y

L

V

I

D

D

T

Q

T

N

I

I

A

L

N

N

G

I

E

P

L

Q

A

L

R

I

D

D

V

-

K

Q

A

Y

D

G

L

V

S

P

V

R

A

A

L

I

F

I

Q

E

F

T

F

W

E

A

Y

A

L

L

P

-

W

-

P

P

A

L

V

S

T

T

S

A

T

T

L

M

A

W

V

G

L

F

V

I

S

L

I

C

F

F

F

I

V

A

T

T

-

V

-

T

-

L

-

I

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

T

T

I

C

A

R

S

S

G

M

G

K

E

E

T

G

A

A

T

D

P

P

A

P

L

L

Q

L

-

V

R

R

I

I

F

G

W

W

C

S

S

V

L

L

S

V

G

G

I

I

V

I

A

G

A

I

V

I

L

L

S

A

T

L

G

G

L

L

T

K

A

P

L

I

Q

Q

S

T

A

A

T

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

S

F

L

F

V

V

M

N

L

I

I

M

L

V

binding 3-carboxy-n,n,n-trimethylpropan-1-aminium: W139 (= W191), W315 (= W365), W316 (= W366)

Query Sequence

>SM_b20333 FitnessBrowser__Smeli:SM_b20333
MQNRVVSCRFTGSTARRATMPEGIRGRSHILFLVPLSRAESVGRLHQVQRFKVNLPVFVG
SVAVIALFVGIGVIAPKRAESIFSGMQTAILSGFGWLYLLSVAVFLFSMLFLAFSRYGEL
KLGPDDSEPEFRYLSWIAMLFAAGMGIGLMYFAVGEPMTHFASPPEAEPLTIAAQREAMS
VTFFHWGVHAWAIYSVVGLSLAYFGYRYNLPLTVRSGLYPLLKEGIHGPIGHVVDIFAIC
GTMFGLATSLGFGILQINSGLNYLLGIPQSIYVQLLLVTVVTAIATISVVTGVEKGVRIL
SETNLFLAVLLMLFVLVVGPTGTLMRDFVQNIGLYLDSLVLRTFNIYAYEPRPWIDSWTL
FYWAWWISWSPFVGMFIARISRGRTVREFVTAVLFVPAMFTFLWMTVFGNTAIYVDTTIA
NGELARDVKADLSVALFQFFEYLPWPAVTSTLAVLLVSIFFVTSSDSGSLVIDTIASGGE
TATPALQRIFWCSLSGIVAAVLLSTGGLTALQSATISTALPFSLVMLILVWSLFVGMRAD
LARTQSPGSLGPRAYPASGVPWQRRLAMTLSTPDRRAVEKFLQASVLPALEAVARELTRR
SRPASVGRDAETGALTLTVPAEGHRDFVYGVQMSEHKLPAFTAYDATVADVRYEARTFFS
DGSRGYDIMGMADNQIINDVLFQFERYTGFVRSPESSLLATSPEER

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory