SitesBLAST
Comparing SM_b20379 FitnessBrowser__Smeli:SM_b20379 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6g4dB Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp (see paper)
55% identity, 97% coverage: 6:453/461 of query aligns to 1:448/453 of 6g4dB
- active site: Y15 (= Y20), Y146 (= Y151), D253 (= D258), K282 (= K287)
- binding pyridoxal-5'-phosphate: S112 (= S117), G113 (= G118), S114 (= S119), N117 (= N122), Y146 (= Y151), H147 (= H152), G148 (= G153), E220 (= E225), D253 (= D258), V255 (= V260), I256 (= I261), K282 (= K287), F318 (= F323), T319 (= T324)
6g4fA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with pmp (see paper)
55% identity, 97% coverage: 6:453/461 of query aligns to 1:448/451 of 6g4fA
- active site: Y15 (= Y20), Y146 (= Y151), D253 (= D258), K282 (= K287)
- binding pyridoxal-5'-phosphate: F318 (= F323), T319 (= T324)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S112 (= S117), G113 (= G118), S114 (= S119), N117 (= N122), Y146 (= Y151), H147 (= H152), G148 (= G153), E220 (= E225), D253 (= D258), V255 (= V260), I256 (= I261), K282 (= K287)
6g4eA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp and 6-aminohexanoate (6-aca) (see paper)
55% identity, 97% coverage: 6:453/461 of query aligns to 1:448/451 of 6g4eA
- active site: Y15 (= Y20), Y146 (= Y151), D253 (= D258), K282 (= K287)
- binding 6-aminohexanoic acid: Y15 (= Y20), W53 (≠ F58), Y146 (= Y151), K282 (= K287), R412 (= R417), M414 (≠ L419)
- binding pyridoxal-5'-phosphate: S112 (= S117), G113 (= G118), S114 (= S119), N117 (= N122), Y146 (= Y151), H147 (= H152), G148 (= G153), E220 (= E225), D253 (= D258), V255 (= V260), I256 (= I261), K282 (= K287), F318 (= F323), T319 (= T324)
5ghgB Transaminase w58l with smba
55% identity, 97% coverage: 5:452/461 of query aligns to 1:429/433 of 5ghgB
- active site: Y16 (= Y20), Y143 (= Y151), E217 (= E225), D250 (= D258), I253 (= I261), K279 (= K287), A401 (≠ S424)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S109 (= S117), G110 (= G118), S111 (= S119), N114 (= N122), Y143 (= Y151), H144 (= H152), G145 (= G153), E217 (= E225), D250 (= D258), V252 (= V260), I253 (= I261), K279 (= K287)
Q94CE5 Gamma-aminobutyrate transaminase POP2, mitochondrial; AtGABA-T; Gamma-aminobutyric acid aminotransferase 1; Protein HEXENAL RESPONSE 1; Protein POLLEN-PISTIL INCOMPATIBILITY 2; AtPOP2; EC 2.6.1.96 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
55% identity, 94% coverage: 16:450/461 of query aligns to 55:491/504 of Q94CE5
- C98 (= C59) mutation to Y: In her1-1; loss of E2-hexenal responses.
- G103 (≠ F64) mutation to N: In her1-2; loss of E2-hexenal responses.
- G271 (= G231) mutation to E: In pop2-6; Loss of activity and complete sterility.
- G312 (= G272) mutation to D: In pop2-7; loss of activity.
D6R3B6 Vanillin aminotransferase; Putative aminotransferase; pAMT; EC 2.6.1.119 from Capsicum frutescens (Cayenne pepper) (Tabasco pepper) (see paper)
55% identity, 91% coverage: 31:450/461 of query aligns to 27:447/459 of D6R3B6
- SAGL 155:158 (≠ TASL 158:161) mutation Missing: Low pungency phenotype, characterized by low levels of capsaicinoids, and accumulation of high levels of capsinoids, which are non-pungent capsaicinoid analogs.
5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
46% identity, 96% coverage: 6:448/461 of query aligns to 7:454/460 of 5kr6B
- binding pyridoxal-5'-phosphate: S121 (= S117), G122 (= G118), S123 (= S119), N126 (= N122), Y155 (= Y151), H156 (= H152), G157 (= G153), E228 (= E225), D261 (= D258), V263 (= V260), V264 (≠ I261), K290 (= K287), W326 (≠ F323), T327 (= T324)
5kr5A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
45% identity, 95% coverage: 11:450/461 of query aligns to 8:452/455 of 5kr5A
- binding calcium ion: E66 (≠ Q67), D70 (≠ E71), D412 (≠ R412), E447 (≠ L445)
- binding pyridoxal-5'-phosphate: S117 (= S117), G118 (= G118), S119 (= S119), N122 (= N122), Y151 (= Y151), H152 (= H152), G153 (= G153), E224 (= E225), D257 (= D258), V259 (= V260), K286 (= K287), W322 (≠ F323), T323 (= T324)
3gjuA Crystal structure of a putative aminotransferase (mll7127) from mesorhizobium loti maff303099 at 1.55 a resolution
43% identity, 98% coverage: 1:452/461 of query aligns to 2:458/458 of 3gjuA
- active site: S21 (≠ Y20), Y154 (= Y151), E228 (= E225), D261 (= D258), V264 (≠ I261), K290 (= K287), T327 (= T324), G430 (≠ S424)
- binding pyridoxal-5'-phosphate: S120 (= S117), G121 (= G118), S122 (= S119), N125 (= N122), Y154 (= Y151), H155 (= H152), G156 (= G153), E228 (= E225), D261 (= D258), V263 (= V260), K290 (= K287)
5kr3A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
43% identity, 97% coverage: 6:450/461 of query aligns to 6:453/458 of 5kr3A
- binding pyridoxal-5'-phosphate: S120 (= S117), G121 (= G118), S122 (= S119), N125 (= N122), Y154 (= Y151), H155 (= H152), G156 (= G153), E225 (= E225), D258 (= D258), V260 (= V260), K287 (= K287), W323 (≠ F323), T324 (= T324)
4e3qA Pmp-bound form of aminotransferase crystal structure from vibrio fluvialis (see paper)
42% identity, 98% coverage: 3:452/461 of query aligns to 1:449/452 of 4e3qA