SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SM_b20379 FitnessBrowser__Smeli:SM_b20379 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6g4dB Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp (see paper)
55% identity, 97% coverage: 6:453/461 of query aligns to 1:448/453 of 6g4dB

query
sites
6g4dB

N

S

S

S

L

L

H

P

A

E

L

K

D

D

K

I

Q

Q

S

Y

L

Q

V

L

H

H

P

P

Y
|
Y

T

T

N

N

L

A

A

R

V

L

H

H

Q

Q

E

E

T

L

G

G

P

P

H

L

V

I

I

I

T

E

G

R

G

G

D

E

G

G

I

I

Y

Y

V

V

V

Y

D

D

D

D

E

Q

G

G

K

K

R

G

Y

Y

I

I

E

E

G

A

L

M

A

A

G

G

L

L

F

W

C

S

A

A

G

A

L

L

G

G

F

F

S

S

E

N

Q

Q

R

R

L

L

V

I

E

K

A

A

A

A

T

E

R

Q

Q

Q

L

F

K

N

T

T

M

L

P

P

F

F

Y

Y

H

H

S

L

F

F

A

S

H

H

K

K

S

S

T

H

E

R

P

P

G

S

I

I

R

E

L

L

A

A

E

E

K

K

L

L

L

I

S

E

I

M

A

A

P

P

V

V

P

P

M

M

S

S

K

K

V

V

F

F

F

F

A

T

G

N

S
|
S

G
|
G

S
|
S

E

E

A

A

N

N

D

D

T

T

A

V

I

V

K

K

L

M

I

V

W

W

Y

Y

Y

L

N

N

N

N

A

A

L

L

G

G

R

K

P

P

E

A

K

K

K

K

K

K

I

F

I

I

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S

R

R

R

V

K

N

A

G

Y
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Y

H
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H

G

G

V

I

T

T

V

V

A

A

T

S

A

A

S

S

L

L

T

T

G

G

L

L

P

P

F

G

N

N

H

Q

R

R

D

G

F

F

D

D

L

L

P

P

I

L

A

P

N

G

I

F

L

L

H

H

T

V

D

G

C

C

P

P

H

H

Y

H

W

Y

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R

F

F

A

A

E

L

T

A

G

G

E

E

T

S

E

E

E

E

D

H

F

F

A

A

T

D

R

R

M

L

A

A

N

V

N

E

L

L

E

E

A

Q

I

K

I

I

L

L

E

A

E

E

G

G

P

P

E

E

T

T

I

I

A

A

A

A

F

F

F

I

A

G

E
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E

P

P

V

L

M

M

V

G

S

A

G

G

G

G

V

V

I

I

T

V

P

P

P

P

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Y

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E

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C

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R

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K

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Y

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D

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I

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L

V

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I

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I
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I

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C

G

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F

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G

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T

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G

N

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M

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F

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S

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F

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P

D

D

M

I

I

M

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C

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A

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K
|
K

Q

Q

L

L

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S

A

S

A

S

Y

Y

M

Q

P

P

I

I

S

A

A

A

L

I

M

L

I

I

N

N

A

A

K

P

I

V

A

F

D

E

A

G

L

I

V

A

D

D

Q

Q

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R

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K

A

I

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T

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F

L

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H

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Y

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H

P

P

V

V

A

A

A

T

A

A

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V

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A

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L

E

E

A

N

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E

A

N

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F

Y

P

Q

P

A

A

L

W

G

Q

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L

A

G

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R

Q

Y

A

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A

K

G

F

R

A

A

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Q

A

E

R

H

G

G

V

M

L

I

T

T

R

R

G

A

L

M

G

G

D

D

M

A

V

V

S

A

L

F

C

C

P

P

A

P

M

L

I

I

I

V

D

N

D

E

E

Q

Q

E

I

V

D

G

D

M

L

I

M

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Q

W

W

active site: Y15 (= Y20), Y146 (= Y151), D253 (= D258), K282 (= K287)
binding pyridoxal-5'-phosphate: S112 (= S117), G113 (= G118), S114 (= S119), Y146 (= Y151), H147 (= H152), E220 (= E225), D253 (= D258), V255 (= V260), I256 (= I261), K282 (= K287)

6g4fA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with pmp (see paper)
55% identity, 97% coverage: 6:453/461 of query aligns to 1:448/451 of 6g4fA

query
sites
6g4fA

N

S

S

S

L

L

H

P

A

E

L

K

D

D

K

I

Q

Q

S

Y

L

Q

V

L

H

H

P

P

Y
|
Y

T

T

N

N

L

A

A

R

V

L

H

H

Q

Q

E

E

T

L

G

G

P

P

H

L

V

I

I

I

T

E

G

R

G

G

D

E

G

G

I

I

Y

Y

V

V

V

Y

D

D

D

D

E

Q

G

G

K

K

R

G

Y

Y

I

I

E

E

G

A

L

M

A

A

G

G

L

L

F

W

C

S

A

A

G

A

L

L

G

G

F

F

S

S

E

N

Q

Q

R

R

L

L

V

I

E

K

A

A

A

A

T

E

R

Q

Q

Q

L

F

K

N

T

T

M

L

P

P

F

F

Y

Y

H

H

S

L

F

F

A

S

H

H

K

K

S

S

T

H

E

R

P

P

G

S

I

I

R

E

L

L

A

A

E

E

K

K

L

L

L

I

S

E

I

M

A

A

P

P

V

V

P

P

M

M

S

S

K

K

V

V

F

F

F

F

A

T

G

N

S
|
S

G
|
G

S
|
S

E

E

A

A

N

N

D

D

T

T

A

V

I

V

K

K

L

M

I

V

W

W

Y

Y

Y

L

N

N

N

N

A

A

L

L

G

G

R

K

P

P

E

A

K

K

K

K

K

K

I

F

I

I

S

S

R

R

R

V

K

N

A

G

Y
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Y

H
|
H

G

G

V

I

T

T

V

V

A

A

T

S

A

A

S

S

L

L

T

T

G

G

L

L

P

P

F

G

N

N

H

Q

R

R

D

G

F

F

D

D

L

L

P

P

I

L

A

P

N

G

I

F

L

L

H

H

T

V

D

G

C

C

P

P

H

H

Y

H

W

Y

R

R

F

F

A

A

E

L

T

A

G

G

E

E

T

S

E

E

E

E

D

H

F

F

A

A

T

D

R

R

M

L

A

A

N

V

N

E

L

L

E

E

A

Q

I

K

I

I

L

L

E

A

E

E

G

G

P

P

E

E

T

T

I

I

A

A

A

A

F

F

F

I

A

G

E
|
E

P

P

V

L

M

M

V

G

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A

G

G

G

G

V

V

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Y

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C

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R

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K

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Y

D

D

I

I

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L

L

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I

I

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D

E

E

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V

I
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I

C

C

G

G

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F

G

G

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R

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T

G

G

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M

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F

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P

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K

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Y

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P

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A

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A

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D

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A

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A

A

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A

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E

E

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C

G

G

L

L

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I

A

A

G

A

L

V

E

E

F

L

V

V

K

G

D

D

K

R

A

V

T

S

R

K

E

A

N

P

F

Y

P

Q

P

A

A

L

W

G

Q

T

V

L

A

G

N

R

Q

Y

A

M

G

A

K

G

F

R

A

A

T

Q

A

E

R

H

G

G

V

M

L

I

T

T

R

R

G

A

L

M

G

G

D

D

M

A

V

V

S

A

L

F

C

C

P

P

A

P

M

L

I

I

I

V

D

N

D

E

E

Q

Q

E

I

V

D

G

D

M

L

I

M

V

T

E

R

R

M

F

G

A

L

R

A

A

L

L

D

D

D

D

T

T

L

T

A

Q

W

W

active site: Y15 (= Y20), Y146 (= Y151), D253 (= D258), K282 (= K287)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S112 (= S117), G113 (= G118), S114 (= S119), Y146 (= Y151), H147 (= H152), E220 (= E225), D253 (= D258), V255 (= V260), I256 (= I261), K282 (= K287)

6g4eA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp and 6-aminohexanoate (6-aca) (see paper)
55% identity, 97% coverage: 6:453/461 of query aligns to 1:448/451 of 6g4eA

query
sites
6g4eA

N

S

S

S

L

L

H

P

A

E

L

K

D

D

K

I

Q

Q

S

Y

L

Q

V

L

H

H

P

P

Y
|
Y

T

T

N

N

L

A

A

R

V

L

H

H

Q

Q

E

E

T

L

G

G

P

P

H

L

V

I

I

I

T

E

G

R

G

G

D

E

G

G

I

I

Y

Y

V

V

V

Y

D

D

D

D

E

Q

G

G

K

K

R

G

Y

Y

I

I

E

E

G

A

L

M

A

A

G

G

L

L

F

W

C

S

A

A

G

A

L

L

G

G

F

F

S

S

E

N

Q

Q

R

R

L

L

V

I

E

K

A

A

A

A

T

E

R

Q

Q

Q

L

F

K

N

T

T

M

L

P

P

F

F

Y

Y

H

H

S

L

F

F

A

S

H

H

K

K

S

S

T

H

E

R

P

P

G

S

I

I

R

E

L

L

A

A

E

E

K

K

L

L

L

I

S

E

I

M

A

A

P

P

V

V

P

P

M

M

S

S

K

K

V

V

F

F

F

F

A

T

G

N

S
|
S

G
|
G

S
|
S

E

E

A

A

N

N

D

D

T

T

A

V

I

V

K

K

L

M

I

V

W

W

Y

Y

Y

L

N

N

N

N

A

A

L

L

G

G

R

K

P

P

E

A

K

K

K

K

K

K

I

F

I

I

S

S

R

R

R

V

K

N

A

G

Y
|
Y

H
|
H

G

G

V

I

T

T

V

V

A

A

T

S

A

A

S

S

L

L

T

T

G

G

L

L

P

P

F

G

N

N

H

Q

R

R

D

G

F

F

D

D

L

L

P

P

I

L

A

P

N

G

I

F

L

L

H

H

T

V

D

G

C

C

P

P

H

H

Y

H

W

Y

R

R

F

F

A

A

E

L

T

A

G

G

E

E

T

S

E

E

E

E

D

H

F

F

A

A

T

D

R

R

M

L

A

A

N

V

N

E

L

L

E

E

A

Q

I

K

I

I

L

L

E

A

E

E

G

G

P

P

E

E

T

T

I

I

A

A

A

A

F

F

F

I

A

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E
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E

P

P

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M

V

G

S

A

G

G

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C

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K

Y

Y

D

D

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L

L

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I

I

A

A

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D

E

E

V
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V

I
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I

C

C

G

G

F

F

G

G

R

R

T

T

G

G

N

Q

M

M

F

F

G

G

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F

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G

L

I

K

Q

P

P

D

D

M

I

I

M

S

V

C

L

A

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K
|
K

Q

Q

L

L

S

S

A

S

A

S

Y

Y

M

Q

P

P

I

I

S

A

A

A

L

I

M

L

I

I

N

N

A

A

K

P

I

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A

F

D

E

A

G

L

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V

A

D

D

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A

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F

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F

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Y

G

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H

H

P

P

V

V

A

A

A

T

A

A

V

V

A

A

L

L

E

E

A

N

L

L

T

K

I

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Y

I

E

E

E

E

I

E

D

S

I

L

V

V

G

E

H

H

V

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R

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A

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Q

A

L

F

L

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R

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G

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E

D

H

H

P

P

L

L

I

V

G

G

E

E

A

I

R

R

G

G

V

C

G

G

L

L

V

I

A

A

G

A

L

V

E

E

F

L

V

V

K

G

D

D

K

R

A

V

T

S

R

K

E

A

N

P

F

Y

P

Q

P

A

A

L

W

G

Q

T

V

L

A

G

N

R

Q

Y

A

M

G

A

K

G

F

R

A

A

T

Q

A

E

R

H

G

G

V

M

L

I

T

T

R
|
R

G

A

L
x
M

G

G

D

D

M

A

V

V

S

A

L

F

C

C

P

P

A

P

M

L

I

I

I

V

D

N

D

E

E

Q

Q

E

I

V

D

G

D

M

L

I

M

V

T

E

R

R

M

F

G

A

L

R

A

A

L

L

D

D

D

D

T

T

L

T

A

Q

W

W

active site: Y15 (= Y20), Y146 (= Y151), D253 (= D258), K282 (= K287)
binding 6-aminohexanoic acid: K282 (= K287), R412 (= R417), M414 (≠ L419)
binding pyridoxal-5'-phosphate: S112 (= S117), G113 (= G118), S114 (= S119), Y146 (= Y151), H147 (= H152), E220 (= E225), D253 (= D258), V255 (= V260), I256 (= I261), K282 (= K287)

5ghgB Transaminase w58l with smba
55% identity, 97% coverage: 5:452/461 of query aligns to 1:429/433 of 5ghgB

query
sites
5ghgB

P

P

N

N

S

S

L

L

H

E

A

A

L

R

D

D

K

I

Q

R

S

Y

L

H

V

L

H

H

P

S

Y
|
Y

T

T

N

D

L

A

A

V

V

R

H

L

Q

E

E

A

T

E

G

G

P

P

H

L

V

V

I

I

T

E

G

R

G

G

D

D

G

G

I

I

Y

Y

V

V

V

E

D

D

D

V

E

S

G

G

K

K

R

R

Y

Y

I

I

E

E

G

A

L

M

A

S

G

G

L

L

F

L

C

S

A

V

G

G

L

V

G

G

F

F

S

S

E

E

Q

P

R

R

L

L

V

A

E

E

A

A

A

A

T

A

R

R

Q

Q

L

M

K

K

T

K

M

L

P

P

F

F

Y

Y

H

H

S

T

F

F

A

H

H

G

K

-

S

-

T

-

E

-

P

P

G

V

I

I

R

D

L

L

A

A

E

E

K

K

L

L

L

V

S

S

I

M

A

A

P

P

V

V

P

P

M

M

S

S

K

K

V

A

F

Y

F

F

A

T

G

N

S
|
S

G
|
G

S
|
S

E

E

A

A

N

N

D

D

T

T

A

V

I

V

K

K

L

L

I

I

W

W

Y

Y

Y

R

N

S

N

N

A

A

L

L

G

G

R

E

P

P

E

E

K

R

K

K

K

K

I

I

I

I

S

S

R

R

R

K

K

R

A

G

Y
|
Y

H
|
H

G

G

V

V

T

T

V

I

A

A

T

S

A

A

S

S

L

L

T

T

G

G

L

L

P

P

F

N

N

N

H

H

R

R

D

S

F

F

D

D

L

L

P

P

I

I

A

D

N

R

I

I

L

L

H

H

T

T

D

G

C

C

P

P

H

H

Y

F

W

Y

R

R

F

E

A

G

E

Q

T

A

G

G

E

E

T

S

E

E

E

E

D

Q

F

F

A

A

T

T

R

R

M

L

A

A

N

D

N

E

L

L

E

E

A

Q

I

L

I

I

L

I

E

A

E

E

G

G

P

P

E

H

T

T

I

I

A

A

A

A

F

F

F

I

A

G

E
|
E

P

P

V

V

M

M

V

G

S

A

G

G

G

G

V

V

I

V

T

V

P

P

P

P

K

K

T

T

Y

Y

F

W

E

E

K

K

V

V

Q

Q

A

A

V

V

L

L

R

K

K

R

Y

Y

D

D

I

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

I
|
I

C

C

G

G

F

F

G

G

R

R

T

T

G

G

N

N

M

L

F

F

G

G

S

S

E

Q

T

T

Y

F

G

D

L

M

K

K

P

P

D

D

M

I

I

L

S

V

C

M

A

S

K
|
K

Q

Q

L

L

S

S

A

S

A

S

Y

Y

M

L

P

P

I

I

S

S

A

A

L

F

M

L

I

I

N

N

A

E

K

R

I

V

A

Y

D

A

A

P

L

I

V

A

D

S

Q

-

S

-

R

-

K

-

I

-

G

-

T

-

F

-

S

-

H

-

G

-

F

-

T

-

Y

-

G

-

G

G

H

H

P

P

V

V

A

A

A

A

A

A

V

V

A

A

L

L

E

E

A

N

L

L

T

A

I

I

Y

I

E

E

E

E

I

R

D

D

I

L

V

V

G

A

H

N

V

A

R

R

S

D

V

R

A

G

P

T

A

Y

F

M

Q

Q

D

K

R

R

V

L

R

R

K

E

L

L

G

Q

E

D

H

H

P

P

L

L

I

V

G

G

E

E

A

V

R

R

G

G

V

V

G

G

L

L

V

I

A

A

G

G

L

V

E

E

F

L

V

V

K

T

D

D

K

K

A

Q

T

A

R

K

E

T

N

G

F

L

P

E

P

P

A

T

W

G

Q

A

V

L

A

G

N

A

Q

K

A

A

G

N

K

A

F

V

A

L

T

Q

A

E

R

R

G

G

V

V

L

I

T

S

R

R

G

A

L

M

G

G

D

D

M

T

V

L

S
x
A

L

F

C

C

P

P

A

P

M

L

I

I

I

I

D

N

D

D

E

Q

Q

Q

I

V

D

D

D

T

L

M

M

V

T

S

R

A

M

L

G

E

L

A

A

T

L

L

D

N

D

D

T

V

L

Q

A

A

active site: Y16 (= Y20), Y143 (= Y151), E217 (= E225), D250 (= D258), I253 (= I261), K279 (= K287), A401 (≠ S424)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S109 (= S117), G110 (= G118), S111 (= S119), Y143 (= Y151), H144 (= H152), E217 (= E225), D250 (= D258), V252 (= V260), I253 (= I261), K279 (= K287)

Q94CE5 Gamma-aminobutyrate transaminase POP2, mitochondrial; AtGABA-T; Gamma-aminobutyric acid aminotransferase 1; Protein HEXENAL RESPONSE 1; Protein POLLEN-PISTIL INCOMPATIBILITY 2; AtPOP2; EC 2.6.1.96 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
55% identity, 94% coverage: 16:450/461 of query aligns to 55:491/504 of Q94CE5

query
sites
Q94CE5

L

M

V

L

H

A

P

P

Y

F

T

T

N

A

L

G

A

W

V

Q

H

S

Q

A

E

D

T

L

G

D

P

P

H

L

V

V

I

I

T

A

G

K

G

S

D

E

G

G

I

S

Y

Y

V

V

V

Y

D

D

D

D

E

T

G

G

K

K

R

K

Y

Y

I

L

E

D

G

S

L

L

A

A

G

G

L

L

F

W

C
|
C

A

T

G

A

L

L

G

G

F
x
G

S

N

E

E

Q

P

R

R

L

L

V

V

E

S

A

A

A

A

T

V

R

E

Q

Q

L

L

K

N

T

T

M

L

P

P

F

F

Y

Y

H

H

S

S

F

F

A

W

H

N

K

R

S

T

T

T

E

K

P

P

G

S

I

L

R

D

L

L

A

A

E

K

K

V

L

L

L

L

S

E

I

M

A

F

P

T

V

A

-

N

P

K

M

M

S

A

K

K

V

A

F

F

F

F

A

T

G

S

S

G

G

G

S

S

E

D

A

A

N

N

D

D

T

T

A

Q

I

V

K

K

L

L

I

V

W

W

Y

Y

Y

Y

N

N

N

N

A

A

L

L

G

G

R

R

P

P

E

E

K

K

K

K

K

K

I

F

I

I

S

A

R

R

R

K

K

K

A

S

Y

Y

H

H

G

G

V

S

T

T

V

L

A

I

T

S

A

A

S

S

L

L

T

S

G

G

L

L

P

P

F

P

N

L

H

H

R

Q

D

N

F

F

D

D

L

L

P

P

I

A

A

P

N

F

I

V

L

L

H

H

T

T

D

D

C

C

P

P

H

H

Y

Y

W

W

R

R

F

F

A

H

E

L

T

P

G

G

E

E

T

T

E

E

E

E

D

E

F

F

A

S

T

T

R

R

M

L

A

A

N

K

N

N

L

L

E

E

A

D

I

L

I

I

L

I

E

K

E

E

G

G

P

P

E

E

T

T

I

I

A

G

A

A

F

F

F

I

A

A

E

E

P

P

V

V

M

M

V

G

S

A

G
|
G

G

G

V

V

I

I

T

P

P

P

P

P

K

A

T

T

Y

Y

F

F

E

E

K

K

V

V

Q

Q

A

A

V

V

L

V

R

K

K

K

Y

Y

D

D

I

I

L

L

L

F

I

I

A

A

D

D

E

E

V

V

I

I

C

C

G

A

F

F

G

G

R

R

T

L

G

G

N

T

M

M

F

F

G
|
G

S

C

E

D

T

K

Y

Y

G

N

L

I

K

K

P

P

D

D

M

L

I

V

S

T

C

L

A

A

K

K

Q

A

L

L

S

S

A

S

A

A

Y

Y

M

M

P

P

I

I

S

G

A

A

L

I

M

L

I

M

N

S

A

Q

K

E

I

V

A

A

D

D

A

V

L

I

V

N

D

S

Q

H

S

S

R

S

K

K

I

L

G

G

T

V

F

F

S

S

H

H

G

G

F

F

T

T

Y

Y

G

S

G

G

H

H

P

P

V

V

A

S

A

C

A

A

V

V

A

A

L

I

E

E

A

A

L

L

T

K

I

I

Y

Y

E

K

E

E

I

R

D

N

I

I

V

P

G

E

H

Y

V

V

R

A

S

K

V

V

A

A

P

P

A

R

F

F

Q

Q

D

D

R

G

V

V

R

K

K

A

L

F

G

A

E

S

-

G

H

S

P

P

L

I

I

I

G

G

E

E

A

T

R

R

G

G

V

T

G

G

L

L

V

I

A

L

G

G

L

T

E

E

F

F

V

V

K

D

D

N

K

K

A

S

T

P

R

N

E

E

N

P

F

F

P

P

P

P

A

E

W

W

Q

G

V

V

A

G

N

A

Q

F

A

F

G

G

K

A

F

E

A

C

T

Q

A

K

R

H

G

G

V

M

L

L

T

V

R

R

G

V

L

A

G

G

D

D

M

G

V

I

S

L

L

M

C

S

P

P

A

P

M

L

I

I

I

I

D

S

D

P

E

E

Q

E

I

I

D

D

D

E

L

L

M

I

T

S

R

I

M

Y

G

G

L

K

A

A

L

L

D

K

D

A

T

T

C98 (= C59) mutation to Y: In her1-1; loss of E2-hexenal responses.
G103 (≠ F64) mutation to N: In her1-2; loss of E2-hexenal responses.
G271 (= G231) mutation to E: In pop2-6; Loss of activity and complete sterility.
G312 (= G272) mutation to D: In pop2-7; loss of activity.

D6R3B6 Vanillin aminotransferase; Putative aminotransferase; pAMT; EC 2.6.1.119 from Capsicum frutescens (Cayenne pepper) (Tabasco pepper) (see paper)
55% identity, 91% coverage: 31:450/461 of query aligns to 27:447/459 of D6R3B6

query
sites
D6R3B6

P

P

H

L

V

V

I

I

T

E

G

K

G

S

D

K

G

G

I

S

Y

Y

V

V

V

Y

D

D

D

I

E

N

G

G

K

K

R

K

Y

Y

I

L

E

D

G

T

L

L

A

S

G

G

L

L

F

W

C

C

A

A

G

T

L

L

G

G

F

G

S

S

E

E

Q

T

R

R

L

L

V

V

E

E

A

A

A

A

T

N

R

K

Q

Q

L

L

K

N

T

T

M

L

P

P

F

F

Y

Y

H

H

S

S

F

F

A

W

H

N

K

R

S

T

T

T

E

K

P

P

G

S

I

L

R

D

L

L

A

A

E

K

K

E

L

L

L

L

S

N

I

M

A

F

P

T

V

A

-

N

P

K

M

M

S

A

K

K

V

V

F

F

F

F

A

T

G

N

S

S

G

G

S

S

E

E

A

A

N

N

D

D

T

T

A

Q

I

V

K

K

L

L

I

V

W

W

Y

Y

Y

Y

N

N

N

N

A

A

L

L

G

G

R

R

P

P

E

Q

K

K

K

K

K

K

I

I

I

I

S

A

R

R

R

A

K

K

A

A

Y

Y

H

H

G

G

V

S

T

T

V

Y

A

I

T
x
S

A
|
A

S
x
G

L
|
L

T

S

G

G

L

L

P

P

F

P

N

M

H

H

R

Q

D

K

F

F

D

D

L

L

P

P

I

P

A

P

N

F

I

V

L

L

H

H

T

T

D

E

C

C

P

P

H

H

Y

Y

W

W

R

A

F

Y

A

H

E

L

T

P

G

G

E

E

T

T

E

E

E

E

D

E

F

F

A

S

T

T

R

R

M

L

A

A

N

N

N

N

L

L

E

E

A

S

I

L

I

I

L

L

E

N

E

E

G

G

P

P

E

E

T

T

I

V

A

A

A

A

F

F

F

I

A

A

E

E

P

P

V

V

M

L

V

G

S

A

G

A

G

G

V

V

I

I

T

L

P

P

P

P

K

A

T

T

Y

Y

F

F

E

D

K

K

V

V

Q

Q

A

A

V

I

L

L

R

R

K

K

Y

H

D

D

I

I

L

L

L

F

I

I

A

A

D

D

E

E

V

V

I

V

C

C

G

G

F

F

G

G

R

R

T

L

G

G

N

T

M

M

F

F

G

G

S

S

E

D

T

K

Y

Y

G

N

L

I

K

K

P

P

D

D

M

L

I

V

S

S

C

V

A

A

K

K

Q

A

L

L

S

S

A

S

A

G

Y

Y

M

M

P

P

I

I

S

A

A

A

L

V

M

L

I

V

N

S

A

Q

K

K

I

I

A

S

D

S

A

V

L

I

V

L

D

S

Q

E

S

S

R

N

K

K

I

I

G

G

T

A

F

F

S

C

H

H

G

G

F

F

T

T

Y

Y

G

S

G

G

H

H

P

P

V

V

A

A

A

C

A

A

V

V

A

A

L

L

E

E

A

A

L

L

T

K

I

I

Y

Y

E

K

E

E

I

R

D

N

I

I

V

T

G

E

H

V

V

V

R

N

S

K

V

I

A

S

P

Q

A

K

F

F

Q

Q

D

E

R

G

V

L

R

K

K

A

L

F

G

A

E

D

H

S

P

P

L

I

I

I

G

G

E

E

A

I

R

R

G

G

V

T

G

G

L

L

V

A

A

L

G

S

L

T

E

E

F

F

V

V

K

D

D

N

K

K

A

S

T

P

R

N

E

D

N

P

F

F

P

P

P

Y

A

E

W

W

Q

A

V

V

A

G

N

T

Q

Y

A

F

G

G

K

A

F

Q

A

C

T

A

A

K

R

Y

G

G

V

M

L

L

T

V

R

S

G

S

L

T

G

G

D

D

M

H

V

V

S

N

L

M

C

A

P

P

A

P

M

F

I

T

I

L

D

S

D

L

E

E

Q

E

I

L

D

D

D

E

L

L

M

I

T

R

R

I

M

Y

G

G

L

K

A

A

L

L

D

K

D

D

T

T

SAGL 155:158 (≠ TASL 158:161) mutation Missing: Low pungency phenotype, characterized by low levels of capsaicinoids, and accumulation of high levels of capsinoids, which are non-pungent capsaicinoid analogs.

5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
46% identity, 96% coverage: 6:448/461 of query aligns to 7:454/460 of 5kr6B

query
sites
5kr6B

N

D

S

D

L

L

H

L

A

E

L

M

D

D

K

R

Q

A

S

H

L

F

V

F

H

H

P

P

Y

S

T

T

N

H

L

L

A

R

V

D

H

H

Q

A

-

S

-

G

E

E

T

L

G

P

P

G

H

R

V

I

I

I

T

T

G

G

G

G

D

K

G

G

I

I

Y

R

V

I

V

Q

D

D

D

S

E

E

G

G

K

R

R

E

Y

Y

I

I

E

D

G

A

L

F

A

A

G

G

L

L

F

Y

C

C

A

V

G

N

L

V

G

G

F

Y

S

G

E

R

Q

T

R

E

L

I

V

A

E

D

A

A

A

I

T

Y

R

E

Q

Q

L

A

K

K

T

E

M

L

P

A

F

Y

Y

Y

H

H

S

T

F

Y

A

V

H

G

K

H

S

S

T

N

E

E

P

P

G

I

I

I

R

E

L

L

A

S

E

E

K

R

L

I

L

I

-

R

S

E

I

W

A

A

P

P

V

A

P

G

M

M

S

S

K

K

V

V

F

Y

F

Y

A

G

G

M

S
|
S

G
|
G

S
|
S

E

D

A

A

N

N

D

E

T

T

A

Q

I

I

K

K

L

L

I

V

W

W

Y

Y

Y

Y

N

N

N

N

A

V

L

L

G

G

R

R

P

P

E

Q

K

K

K

K

K

K

I

I

I

I

S

S

R

R

R

Q

K

R

A

G

Y
|
Y

H
|
H

G
|
G

V

S

T

G

V

I

A

M

T

T

A

G

S

S

L

L

T

T

G

G

L

L

P

P

F

A

N

F

H

H

R

N

D

H

F

F

D

D

L

L

P

P

I

L

A

E

N

P

I

I

L

R

H

H

T

T

D

T

C

C

P

P

H

H

Y

Y

W

Y

R

R

F

-

A

A

E

P

T

A

G

G

E

M

T

S

E

E

E

A

D

E

F

F

A

S

T

R

R

H

M

C

A

A

N

D

N

E

L

L

E

E

A

K

I

M

I

I

L

L

E

A

E

E

G

G

P

P

E

D

T

T

I

V

A

A

A

A

F

F

F

I

A

G

E
|
E

P

P

V

V

M

M

V

G

S

T

G

G

G

G

V

I

I

V

T

P

P

P

P

P

K

E

T

G

Y

Y

F

W

E

E

K

A

V

I

Q

Q

A

A

V

V

L

L

R

N

K

K

Y

Y

D

D

I

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

I

V

C

C

G

G

F

F

G

G

R

R

T

T

G

G

N

S

M

M

F

F

G

G

S

S

E

H

T

H

Y

Y

G

G

L

M

K

K

P

P

D

D

M

L

I

I

S

T

C

V

A

A

K
|
K

Q

G

L

L

S

T

A

S

A

A

Y

Y

M

A

P

P

I

L

S

S

A

G

L

V

M

I

I

V

N

G

A

E

K

K

I

V

A

W

D

K

A

V

L

L

V

E

D

Q

Q

G

S

S

R

D

K

Q

I

Y

G

G

T

P

F

I

S

G

H

H

G

G

F

W

T

T

Y

Y

G

S

G

G

H

H

P

P

V

I

A

C

A

A

A

A

V

A

A

A

L

L

E

A

A

N

L

L

T

D

I

I

Y

I

E

E

E

R

I

E

D

N

I

L

V

T

G

G

H

N

V

A

R

A

S

D

V

T

A

G

P

A

A

Y

F

F

Q

Q

D

Q

R

R

V

M

R

R

K

E

-

T

L

F

G

G

E

D

H

H

P

P

L

L

I

V

G

G

E

E

A

V

R

R

G

G

V

V

G

G

L

L

V

M

A

A

G

A

L

L

E

E

F

F

V

V

K

A

D

D

K

K

A

D

T

K

R

R

E

T

N

R

F

F

P

D

P

P

A

S

W

L

Q

K

V

V

A

G

N

P

Q

R

A

V

G

S

K

A

F

A

A

C

T

L

A

E

R

D

G

G

V

M

L

I

T

A

R

R

G

A

L

M

-

P

-

H

G

G

D

D

M

I

V

L

S

G

L

F

C

A

P

P

A

P

M

L

I

V

I

I

D

T

D

R

E

A

Q

E

I

V

D

D

D

E

L

I

M

V

T

D

R

I

M

A

G

K

L

Q

A

A

L

V

D

D

binding pyridoxal-5'-phosphate: S121 (= S117), G122 (= G118), S123 (= S119), Y155 (= Y151), H156 (= H152), G157 (= G153), E228 (= E225), D261 (= D258), V263 (= V260), K290 (= K287)

5kr5A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
45% identity, 95% coverage: 11:450/461 of query aligns to 8:452/455 of 5kr5A

query
sites
5kr5A

L

M

D

D

K

R

Q

A

S

H

L

F

V

M

H

H

P

P

Y

S

T

T

N

H

L

A

A

Y

V

D

H

H

Q

A

E

S

T

G

G

A

-

L

-

P

P

G

H

R

V

I

I

I

T

T

G

G

G

G

D

K

G

G

I

I

Y

R

V

I

V

E

D

D

D

H

E

E

G

G

K

R

R

E

Y

Y

I

I

E

D

G

A

L

F

A

A

G

G

L

L

F

Y

C

C

A

V

G

N

L

I

G

G

F

Y

S

G

E

R

Q
x
E

R

E

L

V

V

A

E
x
D

A

A

A

I

T

Y

R

E

Q

Q

L

A

K

K

T

Q

M

L

P

A

F

Y

Y

Y

H

H

S

T

F

Y

A

V

H

G

K

H

S

S

T

N

E

D

P

P

G

V

I

I

R

E

L

L

A

S

E

S

K

R

L

I

L

I

S

E

-

D

I

W

A

A

P

P

V

A

P

G

M

M

S

K

K

K

V

V

F

F

F

Y

A

G

G

M

S
|
S

G
|
G

S
|
S

E

D

A

A

N

N

D

E

T

T

A

Q

I

I

K

K

L

L

I

V

W

W

Y

Y

Y

Y

N

N

N

N

A

V

L

L

G

G

R

R

P

P

E

N

K

K

K

K

K

K

I

I

I

I

S

A

R

R

R

E

K

R

A

S

Y
|
Y

H
|
H

G
|
G

V

S

T

G

V

I

A

V

T

T

A

G

S

S

L

L

T

T

G

G

L

L

P

P

F

S

N

F

H

H

R

Q

D

H

F

F

D

D

L

L

P

P

I

I

A

D

N

R

I

V

L

K

H

H

T

T

D

V

C

C

P

P

H

H

Y

-

W

W

R

Y

F

N

A

A

E

P

T

P

G

G

E

M

T

S

E

E

E

A

D

Q

F

F

A

V

T

A

R

Y

M

C

A

V

N

E

N

E

L

L

E

E

A

K

I

L

I

I

L

A

E

R

E

E

G

G

P

A

E

D

T

T

I

I

A

A

A

A

F

F

F

I

A

A

E
|
E

P

P

V

V

M

M

V

G

S

T

G

G

G

G

V

I

I

V

T

P

P

P

P

P

K

Q

T

G

Y

Y

F

W

E

E

K

A

V

I

Q

Q

A

A

V

V

L

L

R

R

K

K

Y

H

D

D

I

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

I

V

C

C

G

G

F

F

G

G

R

R

T

L

G

G

N

S

M

K

F

T

G

G

S

S

E

E

T

H

Y

Y

G

G

L

I

K

K

P

P

D

D

M

L

I

I

S

T

C

V

A

A

K
|
K

Q

G

L

L

S

T

A

S

A

A

Y

Y

M

A

P

P

I

L

S

S

A

A

L

V

M

I

I

V

N

S

A

E

K

K

I

V

A

W

D

D

A

V

L

I

V

E

D

K

Q

G

S

S

R

R

K

E

I

H

G

G

T

V

F

M

S

G

H

H

G

G

F
x
W

T
|
T

Y

Y

G

S

G

G

H

H

P

P

V

V

A

C

A

A

A

A

V

A

A

A

L

L

E

A

A

N

L

L

T

D

I

I

Y

L

E

E

E

R

I

E

D

N

I

L

V

T

G

G

H

N

V

A

R

A

S

D

V

V

A

G

P

A

A

Y

F

L

Q

Q

D

Q

R

R

V

L

R

H

K

E

-

A

L

F

G

G

E

A

H

H

P

P

L

L

I

V

G

G

E

E

A

V

R

R

G

G

V

V

G

G

L

M

V

L

A

A

G

A

L

L

E

E

F

F

V

M

K

A

D

D

K

K

A

G

T

A

R

R

E

T

N

P

F

F

P

D

P

P

A

A

W

L

Q

K

V

V

A

S

N

Q

Q

K

A

V

G

A

K

A

F

A

A

A

T

L

A

E

R
x
D

G

G

V

L

L

I

T

V

R

R

G

A

L

L

-

P

-

H

G

G

D

D

M

I

V

L

S

G

L

F

C

A

P

P

A

P

M

L

I

V

I

T

D

T

D

R

E

A

Q

E

I

V

D

D

D

E

L

I

M

V

T

A

R

I

M

A

G

K

L
x
E

A

A

L

F

D

D

D

E

T

V

binding calcium ion: E66 (≠ Q67), D70 (≠ E71), D412 (≠ R412), E447 (≠ L445)
binding pyridoxal-5'-phosphate: S117 (= S117), G118 (= G118), S119 (= S119), Y151 (= Y151), H152 (= H152), G153 (= G153), E224 (= E225), D257 (= D258), V259 (= V260), K286 (= K287), W322 (≠ F323), T323 (= T324)

3gjuA Crystal structure of a putative aminotransferase (mll7127) from mesorhizobium loti maff303099 at 1.55 a resolution
43% identity, 98% coverage: 1:452/461 of query aligns to 2:458/458 of 3gjuA

query
sites
3gjuA

M

M

N

L

D

N

R

Q

P

S

N

N

S

E

L

L

H

N

A

A

L

W

D

D

K

R

Q

D

S

H

L

F

V

F

H

H

P

P

Y
x
S

T

T

N

H

L

M

A

G

V

T

H

H

Q

A

-

R

-

G

E

E

T

S

G

P

P

T

H

R

V

I

I

M

T

A

G

G

G

G

D

E

G

G

I

V

Y

T

V

V

V

W

D

D

D

N

E

N

G

G

K

R

R

K

Y

S

I

I

E

D

G

A

L

F

A

A

G

G

L

L

F

Y

C

C

A

V

G

N

L

V

G

G

F

Y

S

G

E

R

Q

Q

R

K

L

I

V

A

E

D

A

A

A

I

T

A

R

T

Q

Q

L

A

K

K

T

N

M

L

P

A

F

Y

Y

Y

H

H

S

A

F

Y

A

V

H

G

K

H

S

G

T

T

E

E

P

A

G

S

I

I

R

T

L

L

A

A

E

K

K

M

L

I

L

I

S

D

I

R

A

A

P

P

V

K

P

G

M

M

S

S

K

R

V

V

F

Y

F

F

A

G

G

L

S
|
S

G
|
G

S
|
S

E

D

A

A

N

N

D

E

T

T

A

N

I

I

K

K

L

L

I

I

W

W

Y

Y

Y

Y

N

N

N

N

A

V

L

L

G

G

R

R

P

P

E

E

K

K

K

K

K

K

I

I

I

I

S

S

R

R

R

W

K

R

A

G

Y
|
Y

H
|
H

G

G

V

S

T

G

V

V

A

M

T

T

A

G

S

S

L

L

T

T

G

G

L

L

P

D

F

L

N

F

H

H

R

N

D

A

F

F

D

D

L

L

P

P

I

R

A

A

N

P

I

V

L

L

H

H

T

T

D

E

C

A

P

P

H

Y

Y

Y

W

F

R

R

F

R

A

T

E

D

T

R

G

S

E

M

T

S

E

E

E

E

D

Q

F

F

A

S

T

Q

R

H

M

C

A

A

N

D

N

K

L

L

E

E

A

E

I

M

I

I

L

L

E

A

E

E

G

G

P

P

E

E

T

T

I

I

A

A

A

A

F

F

F

I

A

G

E
|
E

P

P

V

I

M

L

V

G

S

T

G

G

G

G

V

I

I

V

T

P

P

P

P

P

K

A

T

G

Y

Y

F

W

E

E

K

K

V

I

Q

Q

A

A

V

V

L

L

R

K

K

K

Y

Y

D

D

I

V

L

L

L

L

I

V

A

A

D
|
D

E

E

V
|
V

I
x
V

C

T

G

G

F

F

G

G

R

R

T

L

G

G

N

T

M

M

F

F

G

G

S

S

E

D

T

H

Y

Y

G

G

L

I

K

K

P

P

D

D

M

L

I

I

S

T

C

I

A

A

K
|
K

Q

G

L

L

S

T

A

S

A

A

Y

Y

M

A

P

P

I

L

S

S

A

G

L

V

M

I

I

V

N

A

A

D

K

R

I

V

A

W

D

Q

A

V

L

L

V

V

D

Q

Q

G

S

S

R

D

K

K

I

L

G

G

T

S

F

L

S

G

H

H

G

G

F

W

T
|
T

Y

Y

G

S

G

A

H

H

P

P

V

I

A

C

A

V

A

A

V

A

A

G

L

V

E

A

A

N

L

L

T

E

I

L

Y

I

E

D

E

E

I

M

D

D

I

L

V

V

G

T

H

N

V

A

R

G

S

E

V

T

A

G

P

A

A

Y

F

F

Q

R

D

A

R

E

V

L

R

A

K

K

-

A

L

V

G

G

E

G

H

H

P

K

L

N

I

V

G

G

E

E

A

V

R

R

G

G

V

D

G

G

L

M

V

L

A

A

G

A

L

V

E

E

F

F

V

V

K

A

D

D

K

K

A

D

T

D

R

R

E

V

N

F

F

F

P

D

P

A

A

S

W

Q

Q

K

V

I

A

G

N

P

Q

Q

A

V

G

A

K

T

F

A

A

L

T

A

A

A

R

S

G

G

V

V

L

I

T

G

R

R

G

A

L

M

-

P

-

Q

G

G

D

D

M

I

V

L

S
x
G

L

F

C

A

P

P

A

P

M

L

I

C

I

L

D

T

D

R

E

E

Q

Q

I

A

D

D

D

I

L

V

M

V

T

S

R

K

M

T

G

A

L

D

A

A

L

V

D

K

D

S

T

V

L

F

A

A

active site: S21 (≠ Y20), Y154 (= Y151), E228 (= E225), D261 (= D258), V264 (≠ I261), K290 (= K287), T327 (= T324), G430 (≠ S424)
binding pyridoxal-5'-phosphate: S120 (= S117), G121 (= G118), S122 (= S119), Y154 (= Y151), H155 (= H152), E228 (= E225), D261 (= D258), V263 (= V260), K290 (= K287)

5kr3A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
43% identity, 97% coverage: 6:450/461 of query aligns to 6:453/458 of 5kr3A

query
sites
5kr3A

N

D

S

Q

L

L

H

F

A

E

L

M

D

D

K

R

Q

A

S

H

L

F

V

M

H

H

P

P

Y

S

T

T

N

H

L

A

A

H

V

D

H

H

Q

A

E

S

T

G

G

A

-

L

-

P

P

G

H

R

V

I

I

I

T

T

G

G

G

G

D

K

G

G

I

I

Y

R

V

I

V

E

D

D

D

H

E

E

G

G

K

R

R

E

Y

Y

I

I

E

D

G

A

L

F

A

A

G

G

L

L

F

Y

C

C

A

V

G

N

L

I

G

G

F

Y

S

G

E

R

Q

T

R

E

L

V

V

A

E

D

A

A

A

I

T

Y

R

E

Q

Q

L

A

K

K

T

Q

M

L

P

A

F

Y

Y

Y

H

H

S

T

F

Y

A

V

H

G

K

H

S

S

T

T

E

D

P

A

G

I

I

I

R

E

L

L

A

S

E

S

K

R

L

I

L

I

-

R

S

D

I

W

A

A

P

P

V

A

P

G

M

M

S

K

K

K

V

V

F

Y

F

Y

A

G

G

M

S
|
S

G
|
G

S
|
S

E

D

A

A

N

N

D

E

T

T

A

Q

I

I

K

K

L

L

I

V

W

W

Y

Y

Y

Y

N

N

N

N

A

V

L

L

G

G

R

R

P

P

E

N

K

K

K

K

K

K

I

I

I

I

S

S

R

R

R

E

K

R

A

G

Y
|
Y

H
|
H

G

G

V

S

T

G

V

I

A

V

T

T

A

G

S

S

L

L

T

T

G

G

L

L

P

P

F

S

N

F

H

H

R

Q

D

H

F

F

D

D

L

L

P

P

I

I

A

D

N

R

I

V

L

K

H

H

T

T

D

V

C

C

P

P

H

H

Y

W

W

Y

R

R

F

-

A

-

E

-

T

A

G

P

E

M

T

S

E

E

E

A

D

Q

F

F

A

V

T

A

R

Y

M

C

A

V

N

E

N

E

L

L

E

E

A

K

I

L

I

I

L

A

E

R

E

E

G

G

P

A

E

D

T

T

I

I

A

A

A

A

F

F

F

I

A

A

E
|
E

P

P

V

V

M

M

V

G

S

T

G

G

G

G

V

I

I

I

T

P

P

P

P

P

K

Q

T

G

Y

Y

F

W

E

E

K

A

V

I

Q

Q

A

A

V

V

L

L

R

R

K

K

Y

H

D

D

I

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

I

V

C

C

G

G

F

F

G

G

R

R

T

L

G

G

N

S

M

K

F

M

G

G

S

S

E

Q

T

H

Y

Y

G

G

L

I

K

K

P

P

D

D

M

L

I

I

S

T

C

V

A

A

K
|
K

Q

G

L

L

S

T

A

S

A

A

Y

Y

M

A

P

P

I

L

S

S

A

G

L

V

M

I

I

V

N

G

A

E

K

K

I

V

A

W

D

D

A

V

L

I

V

E

D

K

Q

G

S

S

R

Q

K

E

I

H

G

G

T

P

F

M

S

G

H

H

G

G

F

W

T

T

Y

Y

G

S

G

G

H

H

P

P

V

I

A

C

A

A

A

A

V

A

A

A

L

L

E

A

A

N

L

L

T

D

I

I

Y

L

E

E

E

R

I

E

D

N

I

L

V

T

G

G

H

N

V

A

R

A

S

D

V

V

A

G

P

A

A

Y

F

L

Q

Q

D

Q

R

R

V

L

R

H

K

E

-

A

L

F

G

G

E

A

H

H

P

P

L

L

I

V

G

G

E

E

A

V

R

R

G

G

V

V

G

G

L

M

V

L

A

A

G

A

L

L

E

E

F

F

V

M

K

A

D

D

K

K

A

D

T

A

R

R

E

T

N

P

F

F

P

D

P

P

A

A

W

L

Q

K

V

V

A

G

N

P

Q

K

A

V

G

S

K

A

F

A

A

A

T

L

A

E

R

D

G

G

V

M

L

I

T

A

R

R

G

A

L

M

-

P

-

H

G

G

D

D

M

I

V

L

S

G

L

F

C

A

P

P

A

P

M

L

I

V

I

T

D

T

D

R

E

A

Q

E

I

V

D

D

D

E

L

I

M

V

T

G

R

I

M

A

G

K

L

Q

A

A

L

V

D

D

D

E

T

V

binding pyridoxal-5'-phosphate: S120 (= S117), G121 (= G118), S122 (= S119), Y154 (= Y151), H155 (= H152), E225 (= E225), D258 (= D258), V260 (= V260), K287 (= K287)

4e3qA Pmp-bound form of aminotransferase crystal structure from vibrio fluvialis (see paper)
42% identity, 98% coverage: 3:452/461 of query aligns to 1:449/452 of 4e3qA

query
sites
4e3qA

D

N

R

K

P

P

N

Q

S

S

L

W

H

E

A

A

L

R

D

A

K

E

Q

T

S

Y

L

S

V

L

H

Y

P

G

Y
x
F

T

T

N

D

L

M

A

P

V

S

H

L

Q

H

E

Q

T

R

G

G

P

T

H

V

V

V

I

V

T

T

G

H

G

G

D

E

G

G

I

P

Y

Y

V

I

V

V

D

D

D

V

E

N

G

G

K

R

R

R

Y

Y

I

L

E

D

G

A

L

N

A

S

G

G

L

L

F

W

C

N

A

M

G

V

L

A

G

G

F

F

S

D

E

H

Q

K

R

G

L

L

V

I

E

D

A

A

A

A

T

K

R

A

Q

Q

L

Y

K

E

T

R

M

F

P

P

F

G

Y

Y

H

H

S

A

F

F

A

F

H

G

K

R

S

M

T

S

E

D

P

Q

G

T

I

V

R

M

L

L

A

S

E

E

K

K

L

L

L

V

S

E

I

V

A

S

P