SitesBLAST

Q94JQ4 Reactive Intermediate Deaminase A, chloroplastic; 2-iminobutanoate/2-iminopropanoate deaminase; EC 3.5.99.10 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 81% coverage: 19:123/129 of query aligns to 78:185/187 of Q94JQ4

query
sites
Q94JQ4

P

P

F

Y

A

S

R

Q

A

A

V

I

E

K

A

A

G

N

W

L

L

V

H

F

V

L

S

S

G

G

Q

V

V

L

A

G

M

L

-

I

-

P

E

E

N

T

G

G

E

K

I

F

I

V

G

S

G

E

N

S

I

V

V

E

A

D

Q

Q

T

T

H

E

K

Q

A

V

I

L

G

K

N

N

L

M

L

G

A

E

I

I

L

L

K

K

E

A

A

S

G

G

Y

A

G

D

V

Y

E

S

H

S

V

V

R

K

V

T

G

T

V

I

W

M

L

L

D

A

D

D

P

L

R

A

D

D

F

F

W

K

S

T

F

V

N

N

R

E

I

I

Y

Y

Q

A

D

K

Y

Y

F

F

G

P

A

A

H

P

P

S

P

P

A

A

R
|
R

A

S

C

T

V

Y

Q

Q

-

V

S

A

M

L

M

P

V

L

D

N

C

A

K

K

V

I

E

E

I

I

D

E

C

C

V

I

A

A

R165 (= R104) mutation to A: Loss of activity.

P52759 2-iminobutanoate/2-iminopropanoate deaminase; Liver perchloric acid-soluble protein; L-PSP; Reactive intermediate imine deaminase A homolog; Translation inhibitor L-PSP ribonuclease; UK114 antigen homolog; rp14.5; EC 3.5.99.10 from Rattus norvegicus (Rat) (see paper)
34% identity, 82% coverage: 20:125/129 of query aligns to 21:129/137 of P52759

query
sites
P52759

F

Y

A

S

R

Q

A

A

V

V

E

L

A

V

G

D

G

R

W

T

L

I

H

Y

V

V

S

S

G

G

Q

Q

V

I

A

G

M

M

E

D

-

P

-

S

N

S

G

G

E

Q

I

L

I

V

G

P

G

G

N

G

I

V

V

A

A

E

Q

E

T

A

H

K

K

Q

A

A

I

L

G

K

N

N

L

L

L

G

A

E

I

I

L

L

K

K

E

A

A

A

G

G

Y

C

G

D

V

F

E

T

H

N

V

V

R

K

V

T

G

T

V

V

W

L

L

L

D

A

D

D

P

I

R

N

D

D

F

F

W

G

S

T

F

V

N

N

R

E

I

I

Y

Y

Q

K

D

T

Y

Y

F

F

G

Q

A

G

H

N

P

L

P

P

A

A

R

R

A

A

C

A

V

Y

Q

Q

-

V

S

A

M

L

M

P

V

K

D

G

C

S

K

R

V

I

E

E

I

I

D

E

C

A

V

I

A

A

Y

V

K

Q

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

P80601 2-iminobutanoate/2-iminopropanoate deaminase; 14.3 kDa perchloric acid soluble protein; Translation inhibitor L-PSP ribonuclease; UK114 antigen; EC 3.5.99.10; EC 3.1.-.- from Capra hircus (Goat) (see paper)
34% identity, 83% coverage: 19:125/129 of query aligns to 20:129/137 of P80601

query
sites
P80601

P

P

F

Y

A

S

R

Q

A

A

V

V

E

L

A

V

G

D

G

R

W

T

L

I

H

Y

V

I

S

S

G

G

Q

Q

V

L

A

G

M

M

E

D

-

P

-

A

N

S

G

G

E

Q

I

L

I

V

G

P

G

G

N

G

I

V

V

V

A

E

Q

E

T

A

H

K

K

Q

A

A

I

L

G

T

N

N

L

I

L

G

A

E

I

I

L

L

K

K

E

A

A

A

G

G

Y

C

G

D

V

F

E

T

H

N

V

V

R

K

V

A

G

T

V

V

W

L

L

L

D

A

D

D

P

I

R

N

D

D

F

F

W

S

S

A

F

V

N

N

R

D

I

V

Y

Y

Q

K

D

Q

Y

Y

F

F

G

Q

A

S

H

S

P

F

P

P

A

A

R

R

A

A

C

A

V

Y

Q

Q

S

V

S

A

M

A

M

L

V

P

D

K

-

G

C

G

K

R

V

V

E

E

I

I

D

E

C

A

V

I

A

A

Y

V

K

Q

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

5hp8A Crystal structures of rida in complex with pyruvate (see paper)
32% identity, 80% coverage: 21:123/129 of query aligns to 1:106/108 of 5hp8A

query
sites
5hp8A

A

S

R

Q

A

A

V

I

E

K

A

A

G

N

W

L

L

V

H

F

V

L

S

S

G

G

Q

V

V

L

A

G

M

L

-

I

-

P

E

E

N

T

G

G

E

K

I

F

I

V

G

S

G

E

N

S

I

V

V

E

A

D

Q

Q

T

T

H

E

K

Q

A

V

I

L

G

K

N

N

L

M

L

G

A

E

I

I

L

L

K

K

E

A

A

S

G

G

Y

A

G

D

V

Y

E

S

H

S

V

V

R

K

V

T

G

T

V

I

W

M

L

L

D

A

D

D

P

L

R

A

D

D

F

F

W

K

S

T

F

V

N

N

R

E

I

I

Y

Y

Q

A

D

K

Y

Y

F

F

G

P

A

A

H

P

P

S

P

P

A

A

R
|
R

A

S

C
x
T

V

Y

Q

Q

-

V

S

A

M

L

M

P

V

L

D

N

C

A

K

K

V

I

E

E

I

I

D

E

C

C

V

I

A

A

binding pyruvic acid: R86 (= R104), T88 (≠ C106)

2uynA Crystal structure of e. Coli tdcf with bound 2-ketobutyrate (see paper)
30% identity, 88% coverage: 13:125/129 of query aligns to 9:126/127 of 2uynA

query
sites
2uynA

A

A

G

P

G

G

K

A

T

I

L

G

P

P

F

Y

A

V

R

Q

A

G

V

V

E

D

A

L

G

G

G

S

W

M

L

V

H

F

V

T

S

S

G

G

Q

Q

V

I

A

P

M

V

-

C

-

P

E

Q

N

T

G

G

E

E

I

I

I

-

G

P

G

A

N

D

I

V

V

Q

A

D

Q

Q

T

A

H

R

K

L

A

S

I

L

G

E

N

N

L

V

L

K

A

A

I

I

L

V

K

V

E

A

A

A

G

G

Y

L

G

S

V

V

E

G

H

D

V

I

R

K

V

M

G

T

V

V

W

F

L

I

D

T

D

D

P

L

R

N

D

D

F
|
F

W

A

S

T

F

I

N

N

R

E

I

V

Y

Y

Q

K

D

Q

Y

F

F

F

G

D

A

E

H

H

P

Q

-

A

-

T

-

Y

P

P

A

T

R
|
R

A
x
S

C
|
C

V

V

Q

Q

S

V

S

A

M

R

M

L

V

P

-

K

D

D

C

V

K

K

V

L

E

E

I

I

D

E

C

A

V

I

A

A

Y

V

K

R

binding 2-ketobutyric acid: F83 (= F86), R104 (= R104), S105 (≠ A105), C106 (= C106)

2uykC Crystal structure of e. Coli tdcf with bound serine (see paper)
30% identity, 88% coverage: 13:125/129 of query aligns to 9:126/127 of 2uykC

query
sites
2uykC

A

A

G

P

G

G

K

A

T

I

L

G

P

P

F

Y

A

V

R

Q

A

G

V

V

E

D

A

L

G

G

G

S

W

M

L

V

H

F

V

T

S

S

G

G

Q

Q

V

I

A

P

M

V

-

C

-

P

E

Q

N

T

G

G

E

E

I

I

I

-

G

P

G

A

N

D

I

V

V

Q

A

D

Q

Q

T

A

H

R

K

L

A

S

I

L

G

E

N

N

L

V

L

K

A

A

I

I

L

V

K

V

E

A

A

A

G

G

Y

L

G

S

V

V

E

G

H

D

V

I

R

K

V

M

G

T

V

V

W

F

L

I

D

T

D

D

P

L

R

N

D

D

F

F

W

A

S

T

F

I

N

N

R

E

I

V

Y

Y

Q

K

D

Q

Y

F

F

F

G

D

A

E

H

H

P

Q

-

A

-

T

-

Y

P

P

A

T

R
|
R

A
x
S

C
|
C

V

V

Q

Q

S

V

S

A

M

R

M

L

V

P

-

K

D

D

C

V

K

K

V

L

E

E

I

I

D

E

C

A

V

I

A

A

Y

V

K

R

binding serine: R104 (= R104), S105 (≠ A105), C106 (= C106)

3vczB 1.80 angstrom resolution crystal structure of a putative translation initiation inhibitor from vibrio vulnificus cmcp6
30% identity, 83% coverage: 19:125/129 of query aligns to 15:127/127 of 3vczB

query
sites
3vczB

P

P

F

Y

A

I

R

Q

A

G

V

V

E

D

A

L

G

G

G

N

W

M

L

V

H

L

V

T

S

S

G

G

Q

Q

V

I

A

P

M

V

E

N

-

P

-

A

N

T

G

G

E

E

I

V

I

-

G

P

G

A

N

D

I

I

V

A

A

A

Q

Q

T

A

H

R

K

Q

A

S

I

L

G

D

N

N

L

V

L

K

A

A

I

V

L

V

K

E

E

A

A

S

G

G

Y

L

G

T

V

V

E

G

H

D

V

I

V

V

R

K

V

M

G

T

V

V

W

F

L

V

D

K

D
|
D

P

L

R
x
N

D

D

F

F

W

G

S

T

F

V

N

N

R

E

I

V

Y

Y

Q

G

D

N

Y

F

F

F

G

D

-

E

-

H

-

N

-

V

A

A

H

H

P

Y

P

P

A

A

R

R

A

S

C

C

V

V

Q

E

S

V

S

A

M

R

M

L

V

P

-

K

D

D

C

V

K

G

V

I

E

E

I

I

D

E

C

A

V

I

A

A

Y

V

K

R

binding magnesium ion: D79 (= D82), N81 (≠ R84)

A0A1J1DL12 2-iminobutanoate/2-iminopropanoate deaminase; Allergen Der f 34; Enamine/imine deaminase; Allergen Der f 34.0101; EC 3.5.99.10 from Dermatophagoides farinae (American house dust mite) (see paper)
27% identity, 81% coverage: 19:122/129 of query aligns to 18:124/128 of A0A1J1DL12

query
sites
A0A1J1DL12

P

P

F
x
Y

A

S

R

Q

A

A

V

V

E

Q

A

V

G

G

G

N

W

T

L

V

H

Y

V

L

S

S

G
|
G

Q

Q

V

I

A

G

M

M

E

N

-

V

-

R

N

T

G

N

E

E

I

M

I

V

G

T

G

G

N

P

I

I

V

R

A

D

Q

E

T

A

H

Q

K

Q

A

A

I

F

G

T

N
|
N

L

M

L

K

A

A

I

V

L

V

K

E

E

A

A

S

G

G

Y

A

G

K

V

M

E

S

H

D

V

V

R

K

V

V

G

N

V

I

W

F

L

I

D

R

D

N

P

F

R

N

D

D

F

F

W

P

S

A

F

I

N
|
N

R

D

I

V

Y

M

Q

K

D

E

Y

F

F

F

G

Q

A

S

H

P

P

F

P

P

A

A

R
|
R

A

S

C

T

V

V

-

G

Q

V

S

A

S

E

M

L

M
x
P

V

K

D

N

C

A

K

R

V

V

E
|
E

I

I

D

E

C

S

V

I

Y19 (≠ F20) mutation to A: Loss of imine deaminase activity; when associated with A-33; A-59; A-91; A-105; A-114 and A-120.
G33 (= G34) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-59; A-91; A-105; A-114 and A-120.
N59 (= N58) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-91; A-105; A-114 and A-120.
N91 (= N90) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-105; A-114 and A-120.
R105 (= R104) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-114 and A-120.
P114 (≠ M112) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-120.
E120 (= E118) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-114.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5v4dA Crystal structure of the protein of unknown function of the conserved rid protein family yyfa from yersinia pestis
30% identity, 81% coverage: 19:123/129 of query aligns to 17:125/127 of 5v4dA

query
sites
5v4dA

P

P

F

Y

A

S

R

Q

A

A

V

I

E

C

A

F

G

N

G

G

W

I

L

L

H

Y

V

A

S

S

G

G

Q

Q

V

I

A

P

M

I

-

N

-

P

E

D

N

T

G

G

E

D

I

L

I

V

G

E

G

N

N

D

I

I

V

E

A

K

Q

Q

T

T

H

R

K

Q

A

V

I

L

G

K

N

N

L

I

L

D

A

A

I

V

L

L

K

L

E

Q

A

A

G

G

Y

T

G

T

V

K

E

D

H

K

V

I

V

V

R

K

V

T

G

T

V

I

W

F

L

I

D

T

D

N

P

I

R

N

D

N

F

S

W

S

S

Q

F

V

N

N

R
x
D

I

I

Y

Y

Q

A

D
|
D

Y

Y

F

F

-

K

G

G

A

T

H

I

P

F

P

P

A

A

R

R

A

S

C

T

V

V

Q

E

S

V

S

S

M

A

M

L

V

P

D

K

-

G

C

A

K

L

V

V

E

E

I

I

D

E

C

V

V

I

A

A

binding calcium ion: D91 (≠ R91), D95 (= D95)

3k0tC Crystal structure of pspto -psp protein in complex with d-beta-glucose from pseudomonas syringae pv. Tomato str. Dc3000 (see paper)
29% identity, 81% coverage: 19:122/129 of query aligns to 15:120/124 of 3k0tC

query
sites
3k0tC

P

P

F
x
Y

A

S

R

Q

A

A

V

I

E

K

A

A

G

G

G

N

W

T

L

V

H

Y

V

M

S

S

G
|
G

Q

Q

V
x
I

A

P

M

L

E

D

N

P

G

S

-

T

-

M

E

E

I

L

I

V

G

E

G

G

N

-

I

I

V

E

A

A

Q

Q

T

I

H

T

K

Q

A

V

I

F

G

E

N

N

L

L

L

K

A

S

I

V

L

A

K

Q

E

A

A

A

G

G

Y

G

G

S

V

F

E

K

H

D

V

I

V

V

R

K

V

L

G

N

V

I

W

F

L

L

D

T

D

D

P

L

R

G

D

H

F
|
F

W

A

S

K

F

V

N

N

R

E

I

I

Y

M

Q

G

D

S

Y

Y

F

F

G

S

A

Q

H

P

P

Y

P

P

A

A

R
|
R

A

A

C

A

V

I

-

G

Q

V

S

A

M

L

M

P

V
x
R

D

G

C

A

K

Q

V

V

E
|
E

I

M

D

D

C

A

V

I

binding beta-D-glucopyranose: Y16 (≠ F20), G30 (= G34), I32 (≠ V36), F83 (= F86), R101 (= R104), R111 (≠ V113), E116 (= E118)

P0AFQ5 3-aminoacrylate deaminase RutC; 3-AA deaminase; EC 3.5.-.- from Escherichia coli (strain K12) (see paper)
27% identity, 90% coverage: 12:127/129 of query aligns to 10:128/128 of P0AFQ5

query
sites
P0AFQ5

G

G

A

S

G

S

G

A

K

P

T

L

L

A

P

P

F

F

A

V

R

P

A

G

V

T

E

L

A

A

G

D

G

G

W

V

L

V

H

Y

V

V

S

S

G

G

Q

T

V

L

A

A

M

F

E

D

-

Q

-

H

N

N

G

N

E

V

I

L

I

F

G

A

G

D

N

D

I

P

V

K

A

A

Q

Q

T

T

H

R

K

H

A

V

I

L

G

E

N

T

L

I

L

R

A

K

I

V

L

I

K

E

E

T

A

A

G

G

Y

G

G

T

V

M

E

A

H

D

V

V

V

T

R

F

V

N

G

S

V

I

W

F

L

I

D

T

D

D

P

W

R

K

D

N

F

Y

W

A

S

A

F

I

N

N

R

E

I

I

Y

Y

Q

A

D

E

Y

F

F

F

G

P

A

G

H

D

P

K

P

P

A

A

R
|
R

A

F

C

C

V

I

Q

Q

S

C

S

G

M

L

M

V

-

K

V

P

D

D

C

A

K

L

V

V

E

E

I

I

D

A

C

T

V

I

A

A

Y

H

K

I

P

A

K

K

R104 (= R104) mutation to A: Loss of activity.

Query Sequence

>SM_b21157 FitnessBrowser__Smeli:SM_b21157
MSIKRYGAEQVGAGGKTLPFARAVEAGGWLHVSGQVAMENGEIIGGNIVAQTHKAIGNLL
AILKEAGYGVEHVVRVGVWLDDPRDFWSFNRIYQDYFGAHPPARACVQSSMMVDCKVEID
CVAYKPKDA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory