SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SM_b21636 FitnessBrowser__Smeli:SM_b21636 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6khi1 Supercomplex for cylic electron transport in cyanobacteria (see paper)
41% identity, 23% coverage: 275:355/358 of query aligns to 12:91/98 of 6khi1

query
sites
6khi1

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binding fe2/s2 (inorganic) cluster: C41 (= C305), R42 (≠ K306), G44 (= G308), C46 (= C310), C49 (= C313), L77 (= L341), C79 (= C343)

7fixR1 Photosystem I reaction center subunit PsaK (see paper)
41% identity, 23% coverage: 275:355/358 of query aligns to 11:90/97 of 7fixR1

query
sites
7fixR1

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binding [2Ga-2S] cluster: F38 (= F303), S39 (≠ A304), C40 (= C305), R41 (≠ K306), G43 (= G308), C45 (= C310), C48 (= C313), L76 (= L341), C78 (= C343)

6yacN Plant psi-ferredoxin supercomplex (see paper)
41% identity, 24% coverage: 272:358/358 of query aligns to 8:92/97 of 6yacN

query
sites
6yacN

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binding fe2/s2 (inorganic) cluster: Y37 (≠ F303), S38 (≠ A304), C39 (= C305), R40 (≠ K306), C44 (= C310), S46 (= S312), C47 (= C313), C77 (= C343)

7s3dX Structure of photosystem i with bound ferredoxin from synechococcus sp. Pcc 7335 acclimated to far-red light (see paper)
43% identity, 19% coverage: 290:358/358 of query aligns to 25:93/97 of 7s3dX

query
sites
7s3dX

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binding fe2/s2 (inorganic) cluster: Y38 (≠ F303), S39 (≠ A304), C40 (= C305), R41 (≠ K306), G43 (= G308), C45 (= C310), C48 (= C313), C78 (= C343)

P0A3C7 Ferredoxin-1; Ferredoxin I from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
42% identity, 19% coverage: 290:358/358 of query aligns to 27:95/99 of P0A3C7

query
sites
P0A3C7

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R43 (≠ K306) mutation R->A,E,H: No effect on electron transfer.
T49 (≠ S312) mutation to A: No effect on electron transfer.
F66 (≠ A329) mutation F->A,I: 1000-fold decrease in electron transfer.
D69 (= D332) mutation to K: Small effect on electron transfer.
D70 (≠ Y333) mutation to K: Small effect on electron transfer.
E95 (≠ Q358) mutation to K: 20000-fold decrease in electron transfer.

1ewyC Anabaena pcc7119 ferredoxin:ferredoxin-NADP+-reductase complex (see paper)
42% identity, 19% coverage: 290:358/358 of query aligns to 26:94/98 of 1ewyC

query
sites
1ewyC

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binding flavin-adenine dinucleotide: A45 (≠ V309), S64 (≠ N328)
binding fe2/s2 (inorganic) cluster: F39 (= F303), S40 (≠ A304), C41 (= C305), R42 (≠ K306), G44 (= G308), A45 (≠ V309), C46 (= C310), C49 (= C313), L77 (= L341), C79 (= C343)

1czpA Anabaena pcc7119 [2fe-2s] ferredoxin in the reduced and oxixized state at 1.17 a (see paper)
42% identity, 19% coverage: 290:358/358 of query aligns to 26:94/98 of 1czpA

query
sites
1czpA

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binding fe2/s2 (inorganic) cluster: F39 (= F303), C41 (= C305), R42 (≠ K306), G44 (= G308), C46 (= C310), C49 (= C313), C79 (= C343)

7ylrA Structure of a bacteria protein
29% identity, 77% coverage: 81:354/358 of query aligns to 90:325/326 of 7ylrA

query
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7ylrA

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binding fe2/s2 (inorganic) cluster: C276 (= C305), K277 (= K306), G279 (= G308), C281 (= C310), G282 (≠ S311), C284 (= C313), C314 (= C343)
binding flavin mononucleotide: S90 (= S81), T131 (= T125), E229 (= E242), F231 (= F244), E233 (= E257), R278 (≠ A307)

Sites not aligning to the query:

binding flavin mononucleotide: 56, 57, 58, 59, 79, 80, 81, 86, 87, 89

1frrA Crystal structure of [2fe-2s] ferredoxin i from equisetum arvense at 1.8 angstroms resolution (see paper)
42% identity, 20% coverage: 286:358/358 of query aligns to 19:91/95 of 1frrA

query
sites
1frrA

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binding fe2/s2 (inorganic) cluster: C38 (= C305), R39 (≠ K306), G41 (= G308), C43 (= C310), C46 (= C313), C76 (= C343)

1rfkB Crystal structure of 2fe2s ferredoxin from thermophilic cyanobacterium mastigocladus laminosus (see paper)
36% identity, 23% coverage: 275:358/358 of query aligns to 12:94/97 of 1rfkB

query
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1rfkB

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binding fe2/s2 (inorganic) cluster: Y39 (≠ F303), C41 (= C305), R42 (≠ K306), G44 (= G308), C46 (= C310), C49 (= C313), C79 (= C343)

P27787 Ferredoxin-1, chloroplastic; Ferredoxin I; Fd I from Zea mays (Maize) (see 2 papers)
39% identity, 19% coverage: 290:358/358 of query aligns to 76:144/150 of P27787

query
sites
P27787

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D117 (≠ E331) mutation to N: Decreased affinity for Fd-NADP(+) oxidoreductase and decreased electron-transfer activity.

Sites not aligning to the query:

1:52 modified: transit peptide, Chloroplast

3b2gA Leptolyngbya boryana ferredoxin (see paper)
39% identity, 19% coverage: 290:358/358 of query aligns to 26:94/98 of 3b2gA

query
sites
3b2gA

L

I

L

L

E

D

A

A

A

A

L

E

E

E

N

Q

R

G

M

I

D

D

A

L

P

P

F

Y

A
x
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

S

T

C
|
C

R

A

A

G

K

K

V

I

L

T

E

A

G

G

E

T

V

V

E

D

M

Q

E

S

S

D

N

Q

N

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

Q

Q

A

G

G

Y

Y

V

V

L

L

T

T

C
|
C

Q

V

S

A

Y

Y

P

P

L

T

S

S

D

D

R

C

I

T

V

I

V

L

S

T

Y

H

D

Q

Q

E

binding fe2/s2 (inorganic) cluster: S40 (≠ A304), C41 (= C305), R42 (≠ K306), G44 (= G308), C46 (= C310), C49 (= C313), C79 (= C343)

1pfdA The solution structure of high plant parsley [2fe-2s] ferredoxin, nmr, 18 structures (see paper)
39% identity, 19% coverage: 290:358/358 of query aligns to 24:92/96 of 1pfdA

query
sites
1pfdA

L

V

L

L

E

D

A

Q

A

A

L

E

E

E

N

E

R

G

M

I

D

D

A

I

P

P

F

Y

A
x
S

C
|
C

K

R

A

A

G
|
G

V

S

C
|
C

S

S

S

S

C
|
C

R

A

A

G

K

K

V

V

L

V

E

S

G

G

E

S

V

I

E

D

M

Q

E

S

S

D

N

Q

N

S

A

F

L

L

E

D

D

D

Y

E

E

Q

V

M

E

D

Q

A

G

G

Y

Y

V

V

L

L

T

T

C
|
C

Q

H

S

A

Y

Y

P

P

L

T

S

S

D

D

R

V

I

V

V

I

V

E

S

T

Y

H

D

K

Q

E

binding fe2/s2 (inorganic) cluster: S38 (≠ A304), C39 (= C305), G42 (= G308), C44 (= C310), C47 (= C313), C77 (= C343)

1gaqB Crystal structure of the complex between ferredoxin and ferredoxin- NADP+ reductase (see paper)
39% identity, 19% coverage: 290:358/358 of query aligns to 24:92/98 of 1gaqB

query
sites
1gaqB

L

I

L

L

E

D

A

Q

A

A

L

E

E

E

N

D

R

G

M

I

D

D

A

L

P

P

F

Y

A

S

C
|
C

K
x
R

A

A

G
|
G

V

S

C
|
C

S

S

S

S

C
|
C

R

A

A

G

K

K

V

V

L

V

E

S

G

G

E

S

V

V

E

D

M

Q

E

S

S

D

N

Q

N

S

A

Y

L

L

E

D

D

D

Y

G

E

Q

V

I

E

A

Q

D

G

G

Y

W

V

V

L
|
L

T

T

C
|
C

Q

H

S

A

Y

Y

P

P

L

T

S

S

D

D

R

V

I

V

V

I

V

E

S

T

Y

H

D

K

Q

E

binding fe2/s2 (inorganic) cluster: C39 (= C305), R40 (≠ K306), G42 (= G308), C44 (= C310), C47 (= C313), L75 (= L341), C77 (= C343)

2piaA Phthalate dioxygenase reductase: a modular structure for electron transfer from pyridine nucleotides to [2fe-2s] (see paper)
25% identity, 84% coverage: 54:354/358 of query aligns to 54:319/321 of 2piaA

query
sites
2piaA

R

R

R
|
R

S
x
T

Y
|
Y

S
|
S

I

L

C

C

A

N

G

D

L

S

D

Q

E

E

-

R

G

N

A

R

L

Y

K

V

V

I

G
x
A

I
x
V

K

K

R

R

V

D

D

S

D

N

G
|
G

C
x
R

F

G

S
x
G

S
|
S

W

I

A

S

-

F

N

I

E

D

E

D

L

T

E

S

P

E

G

G

D

D

T

A

L

V

E

E

A

V

M

S

P

L

P

P

M

R

G

N

A

E

F

F

F

-

T

-

P

P

I

L

E

D

P

-

E

K

A

R

A

A

K

K

H

S

Y

F

L

I

G

L

F

V

A

A

G

G

G

G

S
x
I

G

G

I

I

T
|
T

P

P

V

M

L

L

S

S

L

M

V

A

K

R

T

Q

V

L

L

R

A

A

R

E

E

G

P

L

R

R

S

S

A

-

F

F

T

R

L

L

V

Y

Y

Y

A

L

N

T

R

R

H

D

F

P

S

E

S

G

I

T

M

A

F

F

R

F

E

D

E

E

L

L

D

-

D

-

L

T

K

S

N

D

L

E

Y

W

L

R

G

S

R

D

L

V

S

K

V

I

L

H

H

H

V

D

L

H

E

G

S

D

E

P

A

T

Q

K

E

A

I

F

D

D

L

F

F

W

S

S

G

-

R

-

L

-

D

-

R

-

E

-

K

-

C

-

T

-

A

-

L

V

F

F

R

E

S

K

W

-

I

-

D

-

V

S

R

K

S

P

A

A

D

Q

T

H

A

V

F

Y

I

C

C

C

G

G

P

P

E

Q

P

A

M

L

M

M

L

D

G

T

V

V

A

R

A

D

A

M

L

T

R

G

A

H

H

W

G

P

L

S

G

G

D

-

D

-

R

T

I

V

K

H

F

F

E
|
E

L

S

F
|
F

A

G

A

A

S

T

Q

N

P

-

G

-

R

-

A

-

R

-

R

-

K

-

V

-

E

-

S

-

A

-

A

-

G

-

A

T

D

N

S

A

R

R

A

E

R

N

C

T

E

P

A

F

T

T

V

V

T

R

L

L

D

S

G

R

A

S

T

G

R

T

S

S

F

F

T

E

F

I

P

P

K

A

E

N

G

-

L

R

S

S

L

I

L

L

E

E

A

V

A

L

L

R

E

D

N

A

R

N

M

V

D

R

A

V

P

P

F
x
S

A

S

C
|
C

K
x
E

A

S

G
|
G

V

T

C
|
C

S
x
G

S

S

C
|
C

R

K

A

T

K

A

V

L

L

C

E

S

G

G

E

E

V

A

E

D

M

H

E

R

S

D

N

M

N

V

A

L

L

R

E

D

D

D

Y

E

E

K

V

G

E

T

Q

Q

G

-

Y

-

V

I

L

M

T

V

C
|
C

Q

V

S

S

Y

R

P

A

L

K

S

S

D

A

R

E

I

L

V

V

V

L

binding fe2/s2 (inorganic) cluster: S270 (≠ F303), C272 (= C305), E273 (≠ K306), G275 (= G308), C277 (= C310), G278 (≠ S311), C280 (= C313), C308 (= C343)
binding flavin mononucleotide: R55 (= R55), T56 (≠ S56), Y57 (= Y57), S58 (= S58), A72 (≠ G71), V73 (≠ I72), G79 (= G78), R80 (≠ C79), G82 (≠ S81), S83 (= S82), I121 (≠ S122), T124 (= T125), E223 (= E242), F225 (= F244)

Sites not aligning to the query:

binding flavin mononucleotide: 44

P33164 Phthalate dioxygenase reductase; PDR; EC 1.-.-.- from Burkholderia cepacia (Pseudomonas cepacia) (see paper)
25% identity, 84% coverage: 54:354/358 of query aligns to 55:320/322 of P33164

query
sites
P33164

R

R

R
|
R

S
x
T

Y

Y

S

S

I

L

C

C

A

N

G

D

L

S

D

Q

E

E

-

R

G

N

A

R

L

Y

K

V

V

I

G
x
A

I
x
V

K
|
K

R

R

V

D

D

S

D

N

G

G

C
x
R

F
x
G

S
x
G

S
|
S

W

I

A

S

-

F

N

I

E

D

E

D

L

T

E

S

P

E

G

G

D

D

T

A

L

V

E

E

A

V

M

S

P

L

P

P

M

R

G

N

A

E

F

F

F

-

T

-

P

P

I

L

E

D

P

-

E

K

A

R

A

A

K

K

H

S

Y

F

L

I

G

L

F

V

A

A

G

G

G

G

S

I

G

G

I

I

T
|
T

P

P

V

M

L

L

S

S

L

M

V

A

K

R

T

Q

V

L

L

R

A

A

R

E

E

G

P

L

R

R

S

S

A

-

F

F

T

R

L

L

V

Y

Y

Y

A

L

N

T

R

R

H

D

F

P

S

E

S

G

I

T

M

A

F

F

R

F

E

D

E

E

L

L

D

-

D

-

L

T

K

S

N

D

L

E

Y

W

L

R

G

S

R

D

L

V

S

K

V

I

L

H

H

H

V

D

L

H

E

G

S

D

E

P

A

T

Q

K

E

A

I

F

D

D

L

F

F

W

S

S

G

-

R

-

L

-

D

-

R

-

E

-

K

-

C

-

T

-

A

-

L

V

F

F

R

E

S

K

W

-

I

-

D

-

V

S

R

K

S

P

A

A

D

Q

T

H

A

V

F

Y

I

C

C

C

G

G

P

P

E

Q

P

A

M

L

M

M

L

D

G

T

V

V

A

R

A

D

A

M

L

T

R

G

A

H

H

W

G

P

L

S

G

G

D

-

D

-

R

T

I

V

K

H

F

F

E

E

L

S

F
|
F

A

G

A

A

S

T

Q

N

P

-

G

-

R

-

A

-

R

-

R

-

K

-

V

-

E

-

S

-

A

-

A

-

G

-

A

T

D

N

S

A

R

R

A

E

R

N

C

T

E

P

A

F

T

T

V

V

T

R

L

L

D

S

G

R

A

S

T

G

R

T

S

S

F

F

T

E

F

I

P

P

K

A

E

N

G

-

L

R

S

S

L

I

L

L

E

E

A

V

A

L

L

R

E

D

N

A

R

N

M

V

D

R

A

V

P

P

F

S

A

S

C
|
C

K

E

A
x
S

G

G

V

T

C
|
C

S

G

S

S

C
|
C

R

K

A

T

K

A

V

L

L

C

E

S

G

G

E

E

V

A

E

D

M

H

E

R

S

D

N

M

N

V

A

L

L

R

E

D

D

D

Y

E

E

K

V

G

E

T

Q

Q

G

-

Y

-

V

I

L

M

T

V

C
|
C

Q

V

S

S

Y

R

P

A

L

K

S

S

D

A

R

E

I

L

V

V

V

L

RT 56:57 (≠ RS 55:56) binding
AVK 73:75 (≠ GIK 71:73) binding
RGGS 81:84 (≠ CFSS 79:82) binding
T125 (= T125) binding
F226 (= F244) binding
C273 (= C305) binding
S275 (≠ A307) binding
C278 (= C310) binding
C281 (= C313) binding
C309 (= C343) binding

1awdA Ferredoxin [2fe-2s] oxidized form from chlorella fusca (see paper)
41% identity, 20% coverage: 286:358/358 of query aligns to 18:90/94 of 1awdA

query
sites
1awdA

E

E

G

D

L

T

S

Y

L

I

L

L

E

D

A

A

A

A

L

E

E

E

N

A

R

G

M

L

D

D

A

L

P

P

F
x
Y

A

S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

S

S

C
|
C

R

A

A

G

K

K

V

V

L

E

E

S

G

G

E

E

V

V

E

D

M

Q

E

S

S

D

N

Q

N

S

A

F

L

L

E

D

D

D

Y

A

E

Q

V

M

E

G

Q

K

G

G

Y

F

V

V

L

L

T

T

C
|
C

Q

V

S

A

Y

Y

P

P

L

T

S

S

D

D

R

V

I

T

V

I

V

L

S

T

Y

H

D

Q

Q

E

binding fe2/s2 (inorganic) cluster: Y35 (≠ F303), C37 (= C305), R38 (≠ K306), G40 (= G308), C42 (= C310), C45 (= C313), C75 (= C343)

P56408 Ferredoxin from Scenedesmus fuscus (Green alga) (Chlorella fusca) (see paper)
41% identity, 20% coverage: 286:358/358 of query aligns to 18:90/94 of P56408

query
sites
P56408

E

E

G

D

L

T

S

Y

L

I

L

L

E

D

A

A

A

A

L

E

E

E

N

A

R

G

M

L

D

D

A

L

P

P

F

Y

A

S

C
|
C

K

R

A

A

G

G

V

A

C
|
C

S

S

S

S

C
|
C

R

A

A

G

K

K

V

V

L

E

E

S

G

G

E

E

V

V

E

D

M

Q

E

S

S

D

N

Q

N

S

A

F

L

L

E

D

D

D

Y

A

E

Q

V

M

E

G

Q

K

G

G

Y

F

V

V

L

L

T

T

C
|
C

Q

V

S

A

Y

Y

P

P

L

T

S

S

D

D

R

V

I

T

V

I

V

L

S

T

Y

H

D

Q

Q

E

C37 (= C305) binding
C42 (= C310) binding
C45 (= C313) binding
C75 (= C343) binding

Sites not aligning to the query:

9 modified: Phosphoserine

4itkA The structure of c.Reinhardtii ferredoxin 2 (see paper)
46% identity, 19% coverage: 290:358/358 of query aligns to 23:90/104 of 4itkA

query
sites
4itkA

L

L

L

L

E

D

A

A

A

A

L

E

E

E

N

A

R

G

M

M

D

D

A

L

P

P

F
x
Y

A

S

C
|
C

K
x
R

A

S

G
|
G

V

G

C
|
C

S

S

S

T

C
|
C

R

C

A

G

K

K

V

L

L

E

E

S

G

G

E

T

V

V

E

D

M

Q

E

S

S

D

N

Q

N

N

A

M

L

L

E

D

D

E

Y

D

E

Q

V

L

E

K

Q

Q

G

G

Y

F

V

V

L

L

T

T

C
|
C

Q

V

S

A

Y

Y

P

P

L

T

S

S

D

D

R

-

I

I

V

V

V

I

S

L

Y

T

D

D

Q

Q

binding fe2/s2 (inorganic) cluster: Y36 (≠ F303), C38 (= C305), R39 (≠ K306), G41 (= G308), C43 (= C310), C46 (= C313), C76 (= C343)

O80429 Ferredoxin-2, chloroplastic; Ferredoxin II; Fd II from Zea mays (Maize) (see 2 papers)
42% identity, 19% coverage: 290:358/358 of query aligns to 68:136/140 of O80429

query
sites
O80429

L

I

L

L

E

D

A

F

A

A

L

E

E

E

N

E

R

G

M

I

D

D

A

L

P

P

F

F

A

S

C

C

K

R

A

A

G

G

V

S

C

C

S

S

S

S

C

C

R

A

A

G

K

K

V

V

L

V

E

S

G

G

E

S

V

V

E

D

M

Q

E

S

S

D

N

Q

N

S

A

F

L

L

E
x
N

D

D

Y

N

E

Q

V

V

E

A

Q

D

G

G

Y

W

V

V

L

L

T

T

C

C

Q

A

S

A

Y

Y

P

P

L

T

S

S

D

D

R

V

I

V

V

I

V

E

S

T

Y

H

D

K

Q

E

N109 (≠ E331) mutation to D: Increased affinity for Fd-NADP(+) oxidoreductase and increased electron-transfer activity.

Sites not aligning to the query:

1:44 modified: transit peptide, Chloroplast

Query Sequence

>SM_b21636 FitnessBrowser__Smeli:SM_b21636
MARFYPLQVTEVRRDTRDAVVVTLEPREEDRAAFDFTQGQYLTFRRLFGGEELRRSYSIC
AGLDEGALKVGIKRVDDGCFSSWANEELEPGDTLEAMPPMGAFFTPIEPEAAKHYLGFAG
GSGITPVLSLVKTVLAREPRSAFTLVYANRHFSSIMFREELDDLKNLYLGRLSVLHVLES
EAQEIDLFSGRLDREKCTALFRSWIDVRSADTAFICGPEPMMLGVAAALRAHGLGDDRIK
FELFAASQPGRARRKVESAAGADSRARCEATVTLDGATRSFTFPKEGLSLLEAALENRMD
APFACKAGVCSSCRAKVLEGEVEMESNNALEDYEVEQGYVLTCQSYPLSDRIVVSYDQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory