SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SM_b21640 FitnessBrowser__Smeli:SM_b21640 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

P76077 1,2-phenylacetyl-CoA epoxidase, subunit A; 1,2-phenylacetyl-CoA epoxidase, catalytic subunit alpha; 1,2-phenylacetyl-CoA monooxygenase, subunit A; EC 1.14.13.149 from Escherichia coli (strain K12) (see paper)
68% identity, 92% coverage: 22:325/332 of query aligns to 3:306/309 of P76077

query
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P76077

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R33 (= R52) binding
Q37 (= Q56) binding
KYSS 103:106 (= KYSS 122:125) binding
N132 (= N151) binding
M193 (= M212) binding
SPN 202:204 (≠ SVH 221:223) binding
K214 (= K233) binding
N218 (= N237) binding

3pw8C The phenylacetyl-coa monooxygenase paaac subcomplex with acetyl-coa (see paper)
68% identity, 90% coverage: 22:321/332 of query aligns to 2:301/301 of 3pw8C

query
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3pw8C

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binding acetyl coenzyme *a: R32 (= R52), Q33 (= Q53), Q36 (= Q56), H37 (= H57), S40 (= S60), E41 (= E61), K102 (= K122), Y103 (= Y123), S104 (= S124), S105 (= S125), F107 (= F127), A128 (= A148), N131 (= N151), M192 (= M212), M193 (= M213), G195 (= G215), P196 (= P216), S201 (= S221), P202 (≠ V222), N203 (≠ H223), K213 (= K233), N217 (= N237), F263 (= F283), I267 (= I287)

3pw1A The phenylacetyl-coa monooxygenase paaac subcomplex with phenylacetyl- coa (see paper)
68% identity, 90% coverage: 22:321/332 of query aligns to 5:304/304 of 3pw1A

query
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3pw1A

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binding Phenylacetyl coenzyme A: R35 (= R52), Q36 (= Q53), Q39 (= Q56), H40 (= H57), S43 (= S60), E44 (= E61), G47 (= G64), E51 (= E68), K70 (= K87), E74 (= E91), K105 (= K122), Y106 (= Y123), S107 (= S124), S108 (= S125), F110 (= F127), V127 (= V144), D128 (= D145), A131 (= A148), N134 (= N151), L138 (= L155), E157 (= E174), M195 (= M212), M196 (= M213), F197 (= F214), G198 (= G215), P199 (= P216), S204 (= S221), P205 (≠ V222), N206 (≠ H223), K216 (= K233), N220 (= N237), F266 (= F283), I270 (= I287)

3pvyA The phenylacetyl-coa monooxygenase paaac subcomplex with coenzyme a (see paper)
68% identity, 90% coverage: 22:321/332 of query aligns to 5:304/304 of 3pvyA

query
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3pvyA

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K

K

binding coenzyme a: R35 (= R52), Q36 (= Q53), Q39 (= Q56), H40 (= H57), S43 (= S60), E44 (= E61), K105 (= K122), Y106 (= Y123), S107 (= S124), S108 (= S125), N134 (= N151), L138 (= L155), M195 (= M212), M196 (= M213), F197 (= F214), G198 (= G215), P199 (= P216), S204 (= S221), P205 (≠ V222), N206 (≠ H223), K216 (= K233), N220 (= N237), F266 (= F283), I270 (= I287)

3pvtA The phenylacetyl-coa monooxygenase paaac subcomplex with 3- hydroxybutanoyl-coa (see paper)
68% identity, 90% coverage: 22:321/332 of query aligns to 5:304/304 of 3pvtA

query
sites
3pvtA

Q

Q

E

E

R

E

A

R

F

F

Q

E

E

Q

R

R

I

I

D

A

A

Q

G

E

Q

T

K

A

I

I

E

E

P

P

K

Q

E

D

W

W

M

M

P

P

E

D

G

A

Y

Y

R

R

K

K

T

T

L

L

I

I

R
|
R

Q
|
Q

I

I

S

G

Q
|
Q

H
|
H

A

A

H

H

S
|
S

E
|
E

I

I

V

V

G

G

Q

M

L

L

P

P

E

E

G

G

N

N

W

W

I

I

T

T

R

R

A

A

P

P

T

T

L

L

E

R

R

R

K

K

A

A

I

I

L

L

A

A

K

K

V

V

Q

Q

D

D

E

E

A

A

G

G

H

H

G

G

L

L

Y

Y

L

L

Y

Y

C

S

A

A

E

E

T

T

L

L

G

G

V

C

S

A

R

R

D

E

A

D

L

I

Y

Y

E

Q

Q

K

L

M

H

L

T

D

G

G

K

R

A

M

K
|
K

Y
|
Y

S
|
S

I

I

F
|
F

N

N

Y

Y

P

P

T

T

L

L

S

S

W

W

A

A

D

D

I

I

G

G

A

V

I

I

G

G

W

W

L

L

V

V

D

D

G

G

A

A

A
|
A

I

I

M

V

N
|
N

Q
|
Q

V

V

P

A

L
|
L

Q

C

R

R

C

T

S

S

Y

Y

G

G

P

P

Y

Y

S

A

R

R

A

A

M

M

I

V

R

K

I

I

C

C

K

K

E

E

S

S

F

F

H

H

Q

Q

R

R

Q

Q

G

G

Y

F

D

E

I

A

L

C

I

M

K

A

M

L

M

A

K

Q

G

G

T

S

P

E

D

A

Q

Q

R

K

A

Q

M

M

V

L

Q

Q

D

D

A

A

L

I

N

N

R

R

W

F

W

W

P

P

S

A

L

L

M
|
M

F
|
F

G
|
G

P
|
P

S

N

D

D

A

N

S
|
S

V
x
P

H
x
N

S

S

A

A

Q

R

S

S

M

L

A

T

W

W

K

K

I

I

K
|
K

Q

R

N

F

S

T

N
|
N

D

D

E

E

L

L

R

R

Q

Q

K

R

F

F

V

V

N

D

Q

N

T

T

V

V

P

P

Q

Q

A

V

E

E

Y

M

L

L

G

G

L

M

T

T

V

V

P

P

D

D

P

P

D

D

L

L

K

H

W

F

N

D

E

T

E

E

K

S

G

G

G

H

Y

Y

D

R

F

F

G

G

E

E

P

I

D

D

W

W

N

Q

E

E

F
|
F

F

N

E

E

V

V

I
|
I

A

N

G

G

N

R

G

G

P

I

C

C

N

N

A

Q

E

E

R

R

L

L

G

D

A

A

R

K

R

R

K

K

A

A

W

W

E

E

D

E

G

G

A

T

W

W

F

V

R

R

D

E

G

A

L

A

T

L

A

A

H

H

A

A

E

Q

K

K

binding 3-hydroxybutanoyl-coenzyme a: R35 (= R52), Q36 (= Q53), Q39 (= Q56), H40 (= H57), S43 (= S60), E44 (= E61), K105 (= K122), Y106 (= Y123), S107 (= S124), S108 (= S125), F110 (= F127), A131 (= A148), N134 (= N151), Q135 (= Q152), L138 (= L155), M195 (= M212), M196 (= M213), F197 (= F214), G198 (= G215), P199 (= P216), S204 (= S221), P205 (≠ V222), N206 (≠ H223), K216 (= K233), N220 (= N237), F266 (= F283), I270 (= I287)

4ii4A The phenylacetyl-coa monooxygenase - mutant paaa e49q k68q - paac wild type subcomplex with benzoyl-coa
68% identity, 90% coverage: 22:321/332 of query aligns to 5:304/304 of 4ii4A

query
sites
4ii4A

Q

Q

E

E

R

E

A

R

F

F

Q

E

E

Q

R

R

I

I

D

A

A

Q

G

E

Q

T

K

A

I

I

E

E

P

P

K

Q

E

D

W

W

M

M

P

P

E

D

G

A

Y

Y

R

R

K

K

T

T

L

L

I

I

R
|
R

Q
|
Q

I

I

S

G

Q
|
Q

H
|
H

A

A

H

H

S
|
S

E

E

I

I

V

V

G

G

Q

M

L

L

P

P

E

Q

G

G

N

N

W

W

I

I

T

T

R

R

A

A

P

P

T

T

L

L

E

R

R

R

K

K

A

A

I

I

L

L

A

A

K

Q

V

V

Q

Q

D

D

E

E

A

A

G

G

H

H

G

G

L

L

Y

Y

L

L

Y

Y

C

S

A

A

E

E

T

T

L

L

G

G

V

C

S

A

R

R

D

E

A

D

L

I

Y

Y

E

Q

Q

K

L

M

H

L

T

D

G

G

K

R

A

M

K
|
K

Y
|
Y

S
|
S

I

I

F

F

N

N

Y

Y

P

P

T

T

L

L

S

S

W

W

A

A

D

D

I

I

G

G

A

V

I

I

G

G

W

W

L

L

V

V

D

D

G

G

A

A

I

I

M

V

N
|
N

Q

Q

V

V

P

A

L

L

Q

C

R

R

C

T

S

S

Y

Y

G

G

P

P

Y
|
Y

S

A

R

R

A

A

M

M

I

V

R

K

I

I

C

C

K

K

E

E

S

S

F

F

H

H

Q

Q

R

R

Q

Q

G

G

Y

F

D

E

I

A

L

C

I

M

K

A

M

L

M

A

K

Q

G

G

T

S

P

E

D

A

Q

Q

R

K

A

Q

M

M

V

L

Q

Q

D

D

A

A

L

I

N

N

R

R

W

F

W

W

P

P

S

A

L

L

M
|
M

F

F

G
|
G

P
|
P

S

N

D

D

A

N

S
|
S

V
x
P

H
x
N

S
|
S

A

A

Q

R

S

S

M

L

A

T

W

W

K

K

I

I

K
|
K

Q

R

N

F

S

T

N
|
N

D

D

E

E

L

L

R

R

Q

Q

K

R

F

F

V

V

N

D

Q

N

T

T

V

V

P

P

Q

Q

A

V

E

E

Y

M

L

L

G

G

L

M

T

T

V

V

P

P

D

D

P

P

D

D

L

L

K

H

W

F

N

D

E

T

E

E

K

S

G

G

G

H

Y

Y

D

R

F

F

G

G

E

E

P

I

D

D

W

W

N

Q

E

E

F
|
F

F

N

E

E

V

V

I
|
I

A

N

G

G

N

R

G

G

P

I

C

C

N

N

A

Q

E

E

R

R

L

L

G

D

A

A

R

K

R

R

K

K

A

A

W

W

E

E

D

E

G

G

A

T

W

W

F

V

R

R

D

E

G

A

L

A

T

L

A

A

H

H

A

A

E

Q

K

K

binding benzoyl coenzyme A: R35 (= R52), Q36 (= Q53), Q39 (= Q56), H40 (= H57), S43 (= S60), K105 (= K122), Y106 (= Y123), S107 (= S124), S108 (= S125), N134 (= N151), Y146 (= Y163), M195 (= M212), M196 (= M213), G198 (= G215), P199 (= P216), S204 (= S221), P205 (≠ V222), N206 (≠ H223), S207 (= S224), K216 (= K233), N220 (= N237), F266 (= F283), I270 (= I287)

Query Sequence

>SM_b21640 FitnessBrowser__Smeli:SM_b21640
MYAQMVKTDAPRVKSPDDMDPQERAFQERIDAGQKIEPKEWMPEGYRKTLIRQISQHAHS
EIVGQLPEGNWITRAPTLERKAILLAKVQDEAGHGLYLYCAAETLGVSRDALYEQLHTGK
AKYSSIFNYPTLSWADIGAIGWLVDGAAIMNQVPLQRCSYGPYSRAMIRICKEESFHQRQ
GYDILIKMMKGTPDQRAMVQDALNRWWWPSLMMFGPSDDASVHSAQSMAWKIKQNSNDEL
RQKFVNQTVPQAEYLGLTVPDPDLKWNEEKGGYDFGEPDWNEFFEVIAGNGPCNAERLGA
RRKAWEDGAWFRDGLTAHAEKAALRAQPVAAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory