SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMa0196 FitnessBrowser__Smeli:SMa0196 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3dr2A Structural and functional analyses of xc5397 from xanthomonas campestris: a gluconolactonase important in glucose secondary metabolic pathways (see paper)
43% identity, 90% coverage: 22:294/304 of query aligns to 32:296/299 of 3dr2A

query
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3dr2A

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binding calcium ion: E45 (= E35), N131 (= N121), N188 (= N179), D236 (= D233)

3e5zA X-ray structure of the putative gluconolactonase in protein family pf08450. Northeast structural genomics consortium target drr130.
39% identity, 95% coverage: 12:300/304 of query aligns to 7:287/290 of 3e5zA

query
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3e5zA

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binding magnesium ion: E30 (= E35), N117 (= N121), N174 (= N179), D220 (= D233)

Q9A9Z1 D-xylonolactone lactonase; Xylono-1,5-lactonase; EC 3.1.1.110 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see paper)
29% identity, 86% coverage: 31:290/304 of query aligns to 7:255/289 of Q9A9Z1

query
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Q9A9Z1

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E17 (= E35) binding
N145 (= N179) binding
D195 (= D233) binding

7pldB Caulobacter crescentus xylonolactonase with (r)-4-hydroxy-2- pyrrolidone (see paper)
29% identity, 86% coverage: 31:290/304 of query aligns to 7:255/289 of 7pldB

query
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7pldB

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binding (R)-4-hydroxy-2-pyrrolidone: L15 (vs. gap), E17 (= E35), I31 (= I51), A64 (≠ R82), G66 (= G84), F67 (≠ R85), P80 (≠ R98), R98 (≠ S119), N100 (= N121), E119 (= E145), D138 (≠ V172), N145 (= N179), K180 (≠ G221), D195 (= D233), W210 (vs. gap), W210 (vs. gap)
binding fe (ii) ion: E17 (= E35), N145 (= N179), D195 (= D233)

7plbB Caulobacter crescentus xylonolactonase with d-xylose (see paper)
29% identity, 86% coverage: 31:290/304 of query aligns to 7:255/289 of 7plbB

query
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7plbB

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H

D

D

Y

G

E

E

G

-

Y

-

H

-

A

-

D

-

P

-

E

E

Q

N

P

N

T

S

R

G

N

S

V

L

Y

Y

R

R

L

M

D
|
D

P

-

E

L

T

T

G

G

E

-

L

V

S

A

A

R

V

M

V

D

T

R

D

D

F

I

T

C

Q

I

P

T

N
|
N

G

G

L

P

A

C

F

V

S

S

P

P

D

D

E

G

K

K

I

T

L

F

Y

Y

V

-

A

-

D

-

S

-

A

-

S

-

H

H

D

T

D

D

R

T

L

L

P

E

R

K

H

T

I

I

R

Y

A

A

F

F

D

D

L

L

T

A

D

E

G
x
D

G

G

R

L

L

L

A

S

N

N

G

K

R

R

V

V

F

F

C

V

V

Q

I

F

D

A

K

L

G

G

-

D

-

V

I

Y

P

P

D
|
D

G

G

I

S

R

V

T

V

D

D

A

S

N

E

G

G

N

Y

L

L

W

W

S

T

S

A

-

L

-

W

A

G

G

G

D

F

G

G

V

A

H

V

C

R

F

F

D

S

T

P

A

Q

G

G

K

D

L

A

I

V

G

T

K

R

I

I

R

E

V

L

P

P

Q

A

-

P

T

N

V

V

A

T

N

K

L

P

T

C

F

F

G

G

P

P

R

D

R

L

N

K

R

T

L

L

F

Y

I

F

A

T

T

A

R

R

S

K

binding fe (ii) ion: E17 (= E35), N145 (= N179), D195 (= D233)
binding beta-D-xylopyranose: L15 (vs. gap), E17 (= E35), E89 (≠ D110), V90 (≠ R111), D92 (≠ E113), R98 (≠ S119), N100 (= N121), R109 (≠ T130), D130 (= D162), N145 (= N179), D174 (≠ G215), D195 (= D233)
binding alpha-D-xylopyranose: A64 (≠ R82), G66 (= G84), F67 (≠ R85), P80 (≠ R98)

Q15493 Regucalcin; RC; Gluconolactonase; GNL; Senescence marker protein 30; SMP-30; EC 3.1.1.17 from Homo sapiens (Human) (see 2 papers)
27% identity, 87% coverage: 27:289/304 of query aligns to 10:264/299 of Q15493

query
sites
Q15493

L

L

Y

P

S

E

G

N

C

C

R

R

W

C

A

G

E
|
E

G

S

P

P

V

V

W

W

F

E

N

E

D

V

A

S

N

N

Q

S

L

L

W

F

S

V

D

D

I

I

P

P

N

A

Q

K

R

K

I

V

L

C

R

R

W

W

-

D

T

S

P

F

E

T

G

K

G

Q

V

V

S

Q

V

R

Y

V

R

T

Q

M

P

D

S

A

N

P

F

V

T

S

N

S

G

V

H

A

T

L

R

R

D

Q

R

S

R

G

G

G

R

Y

L

V

I

A

S

T

C

I

E

-

H

-

G

G

T

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

V

K

D

E

G

Q

S

S

I

A

T

V

V

V

L

L

A

A

D

T

R

V

F

D

E

N

G

D

R

K

L

N

N

N

S

R

P

F

N
|
N

D

D

V

G

V

K

V

V

K

D

S

P

D

A

G

G

T

R

I

-

W

Y

F

F

T

A

D

-

P

-

T

-

Y

-

G

G

I

T

M

M

S

A

D

E

Y

-

E

E

G

T

Y

A

H

P

A

A

D

V

P

L

E

E

Q

R

P

H

T

Q

R

G

N

A

V

L

Y

Y

R

S

L

L

D

F

P

P

E

D

T

H

G

-

E

H

L

V

S

K

A

K

V

Y

V

F

T

D

D

Q

F

V

T

D

Q

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

K

K

I

I

L

F

Y

Y

V

Y

A

I

D

D

S

S

S

-

A

-

S

-

H

-

D

-

R

-

L

L

P

S

R

Y

H

S

I

V

R

D

A

A

F

F

D

D

L

Y

-

D

T

L

D

Q

G

T

G

G

R

Q

L

I

A

S

N

N

G

R

R

R

V

S

F

V

C

Y

V

K

I

L

D

E

K

K

-

E

G

Q

I

I

P

P

D
|
D

G

G

I

M

R

C

T

I

D

D

A

A

N

E

G

G

N

K

L

L

W

W

S

V

S

A

A

C

G

Y

D

N

G

G

-

G

-

R

-

V

V

I

H

R

C

L

F

D

D

P

T

V

A

T

G

G

K

K

L

R

I

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

Q

D

T

K

V

T

A

T

N

S

L

C

T

C

F

F

G

G

P

K

R

N

R

Y

N

S

R

E

L

M

F

Y

I

V

A

T

A

C

T

A

R

R

E18 (= E35) binding ; mutation to A: Reduces enzyme activity by about 90%.
N103 (= N121) mutation to A: Reduces enzyme activity by about 95%.
N154 (= N179) binding ; mutation to A: Reduces enzyme activity by about 95%.
D204 (= D233) binding ; mutation to A: Reduces enzyme activity by over 98%.

4gncA Human smp30/gnl-1,5-ag complex (see paper)
27% identity, 87% coverage: 27:289/304 of query aligns to 9:263/298 of 4gncA

query
sites
4gncA

L

L

Y

P

S

E

G

N

C

C

R

R

W

C

A

G

E
|
E

G

S

P

P

V

V

W

W

F

E

N

E

D

V

A

S

N

N

Q

S

L

L

W

F

S

V

D

D

I

I

P

P

N

A

Q

K

R

K

I

V

L

C

R

R

W

W

-

D

T

S

P

F

E

T

G

K

G

Q

V

V

S

Q

V

R

Y

V

R

T

Q

M

P

D

S

A

N

P

F

V

T

S

N

S

G

V

H

A

T

L

R

R

D

Q

R

S

R

G

G

G

R

Y

L

V

I

A

S

T

C

I

E

-

H

-

G

G

T

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

V

K

D

E

G

Q

S

S

I

A

T

V

V

V

L

L

A

A

D

T

R

V

F

D

E

N

G

D

R

K

L

N

N

N

S
x
R

P

F

N
|
N

D
|
D

V

G

V

K

V

V

K

D

S

P

D

A

G

G

T

R

I

-

W

Y

F

F

T

A

D

-

P

-

T

-

Y

-

G

G

I

T

M

M

S

A

D

E

Y

-

E

E

G

T

Y

A

H

P

A

A

D

V

P

L

E

E

Q

R

P

H

T

Q

R

G

N

A

V

L

Y

Y

R

S

L

L

D

F

P

P

E

D

T

H

G

-

E

H

L

V

S

K

A

K

V

Y

V

F

T

D

D

Q

F

V

T

D

Q

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

K

K

I

I

L

F

Y

Y

V

Y

A

I

D

D

S

S

S

-

A

-

S

-

H

-

D

-

R

-

L

L

P

S

R

Y

H

S

I

V

R

D

A

A

F

F

D

D

L

Y

-

D

T

L

D

Q

G

T

G

G

R

Q

L

I

A

S

N

N

G

R

R

R

V

S

F

V

C

Y

V

K

I

L

D

E

K

K

-

E

G

Q

I

I

P

P

D
|
D

G

G

I

M

R

C

T

I

D

D

A

A

N

E

G

G

N

K

L

L

W

W

S

V

S

A

A

C

G

Y

D

N

G

G

-

G

-

R

-

V

V

I

H

R

C

L

F

D

D

P

T

V

A

T

G

G

K

K

L

R

I

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

Q

D

T

K

V

T

A
x
T

N

S

L

C

T

C

F

F

G

G

P

K

R
x
N

R
x
Y

N

S

R

E

L

M

F

Y

I

V

A

T

A

C

T

A

R

R

binding 1,5-anhydro-D-glucitol: E17 (= E35), R100 (≠ S119), N102 (= N121), D203 (= D233), T245 (≠ A271), N253 (≠ R279), Y254 (≠ R280)
binding calcium ion: E17 (= E35), D103 (= D122), N153 (= N179), D203 (= D233)

Sites not aligning to the query:

binding 1,5-anhydro-D-glucitol: 290, 291

3g4hA Crystal structure of human senescence marker protein-30 (zinc bound) (see paper)
27% identity, 87% coverage: 27:289/304 of query aligns to 8:262/297 of 3g4hA

query
sites
3g4hA

L

L

Y

P

S

E

G

N

C

C

R

R

W

C

A

G

E
|
E

G

S

P

P

V

V

W

W

F

E

N

E

D

V

A

S

N

N

Q

S

L

L

W

F

S

V

D

D

I

I

P

P

N

A

Q

K

R

K

I

V

L

C

R

R

W

W

-

D

T

S

P

F

E

T

G

K

G

Q

V

V

S

Q

V

R

Y

V

R

T

Q

M

P

D

S

A

N

P

F

V

T

S

N

S

G

V

H

A

T

L

R

R

D

Q

R

S

R

G

G

G

R

Y

L

V

I

A

S

T

C

I

E

-

H

-

G

G

T

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

V

K

D

E

G

Q

S

S

I

A

T

V

V

V

L

L

A

A

D

T

R

V

F

D

E

N

G

D

R

K

L

N

N

N

S

R

P

F

N

N

D

D

V

G

V

K

V

V

K

D

S

P

D

A

G

G

T

R

I

-

W

Y

F

F

T

A

D

-

P

-

T

-

Y

-

G

G

I

T

M

M

S

A

D

E

Y

-

E

E

G

T

Y

A

H

P

A

A

D

V

P

L

E

E

Q

R

P

H

T

Q

R

G

N

A

V

L

Y

Y

R

S

L

L

D

F

P

P

E

D

T

H

G

-

E

H

L

V

S

K

A

K

V

Y

V

F

T

D

D

Q

F

V

T

D

Q

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

K

K

I

I

L

F

Y

Y

V

Y

A

I

D

D

S

S

S

-

A

-

S

-

H

-

D

-

R

-

L

L

P

S

R

Y

H

S

I

V

R

D

A

A

F

F

D

D

L

Y

-

D

T

L

D

Q

G

T

G

G

R

Q

L

I

A

S

N

N

G

R

R

R

V

S

F

V

C

Y

V

K

I

L

D

E

K

K

-

E

G

Q

I

I

P

P

D
|
D

G

G

I

M

R

C

T

I

D

D

A

A

N

E

G

G

N

K

L

L

W

W

S

V

S

A

A

C

G

Y

D

N

G

G

-

G

-

R

-

V

V

I

H

R

C

L

F

D

D

P

T

V

A

T

G

G

K

K

L

R

I

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

Q

D

T

K

V

T

A

T

N

S

L

C

T

C

F

F

G

G

P

K

R

N

R

Y

N

S

R

E

L

M

F

Y

I

V

A

T

A

C

T

A

R

R

binding zinc ion: E16 (= E35), N152 (= N179), D202 (= D233)

4gnaA Mouse smp30/gnl-xylitol complex (see paper)
28% identity, 85% coverage: 32:289/304 of query aligns to 13:262/297 of 4gnaA

query
sites
4gnaA

R

R

W

C

A

G

E
|
E

G

S

P

P

V

V

W

W

F

E

N

E

D

A

A

S

N

Q

Q

S

L

L

W

F

S

V

D

D

I

I

P

P

N

S

Q

K

R

I

I

I

L

C

R

R

W

W

-

D

T

T

P

V

E

S

G

N

G

Q

V

V

S

Q

V

R

Y

V

R

A

Q

V

P

D

S

A

N

P

F

V

T

S

N

S

G

V

H

A

T

L

R

R

D

Q

R

L

R

G

G

G

R

Y

L

V

I

A

S

T

C

I

E

-

H

-

G

G

T

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

V

E

D

N

G

Q

S

S

I

V

T

F

V

V

L

L

A

A

D

M

R

V

F

D

E

E

G

D

R

K

L

N

N

N

S
x
R

P

F

N
|
N

D

D

V

G

V

K

V

V

K

D

S

P

D

A

G

G

T

R

I

-

W

Y

F

F

T

A

D

-

P

-

T

-

Y

-

G

G

I

T

M

M

S

A

D

E

Y

-

E
|
E

G

T

Y

A

H

P

A

A

D

V

P

L

E

E

Q

R

P

H

T

Q

R

G

N

S

V

L

Y

Y

R

S

L

L

D

F

P

P

E

D

T

-

G

-

E

-

L

-

S

H

A

S

V

V

V

K

T

K

D

Y

F

F

T

D

Q

Q

-

V

-

D

-

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

K

K

I

I

L

F

Y

Y

V

Y

A

I

D

D

S

S

S

-

A

-

S

-

H

-

D

-

R

-

L

L

P

S

R

Y

H

T

I

V

R

D

A

A

F

F

D

D

L

Y

-

D

T

L

D

Q

G

T

G

G

R

Q

L

I

A

S

N

N

G

R

R

R

V

I

F

V

C

Y

V

K

I

M

D

E

K

K

-

D

-

E

G

Q

I

I

P

P

D
|
D

G

G

I

M

R

C

T

I

D

D

A

A

N

E

G

G

N

K

L

L

W

W

S

V

S

A

A

C

G
x
Y

D

N

G

G

-

G

-

R

-

V

V

I

H

R

C

L

F

D

D

P

T

E

A

T

G

G

K

K

L

R

I

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

Q

D

T

K

V

T

A

T

N

S

L

C

T

C

F

F

G

G

P

K

R

D

R

Y

N

S

R

E

L

M

F

Y

I

V

A

T

A

C

T

A

R

R

binding calcium ion: E16 (= E35), N152 (= N179), D202 (= D233)
binding Xylitol: E16 (= E35), R99 (≠ S119), N101 (= N121), E119 (= E145), N152 (= N179), D202 (= D233), Y217 (≠ G248)

4gn9A Mouse smp30/gnl-glucose complex (see paper)
28% identity, 85% coverage: 32:289/304 of query aligns to 13:262/297 of 4gn9A

query
sites
4gn9A

R

R

W

C

A

G

E
|
E

G

S

P

P

V

V

W

W

F

E

N

E

D

A

A

S

N

Q

Q

S

L

L

W

F

S

V

D

D

I

I

P

P

N

S

Q

K

R

I

I

I

L

C

R

R

W

W

-

D

T

T

P

V

E

S

G

N

G

Q

V

V

S

Q

V

R

Y

V

R

A

Q

V

P

D

S

A

N

P

F

V

T

S

N

S

G

V

H

A

T

L

R

R

D

Q

R

L

R

G

G
|
G

R

Y

L

V

I

A

S

T

C

I

E

-

H

-

G

G

T

T

R

K

R

F

V

C

T

A

R

L

T

N

E
x
W

V
x
E

D

N

G

Q

S

S

I

V

T

F

V

V

L

L

A

A

D

M

R

V

F
x
D

E
|
E

G

D

R

K

L

N

N

N

S
x
R

P

F

N
|
N

D

D

V

G

V

K

V

V

K

D

S

P

D

A

G

G

T

R

I

-

W

Y

F

F

T

A

D

-

P

-

T

-

Y

-

G

G

I

T

M

M

S

A

D

E

Y

-

E
|
E

G

T

Y

A

H

P

A

A

D

V

P

L

E

E

Q

R

P

H

T

Q

R

G

N

S

V

L

Y
|
Y

R

S

L

L

D

F

P

P

E

D

T

-

G

-

E

-

L

-

S

H

A

S

V

V

V

K

T
x
K

D

Y

F

F

T

D

Q

Q

-

V

-

D

-

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

K

K

I

I

L

F

Y

Y

V

Y

A

I

D

D

S
|
S

S

-

A

-

S

-

H

-

D

-

R

-

L

L

P

S

R

Y

H

T

I

V

R

D

A

A

F

F

D

D

L

Y

-

D

T

L

D

Q

G

T

G

G

R

Q

L

I

A

S

N

N

G

R

R

R

V

I

F

V

C

Y

V

K

I

M

D

E

K

K

-

D

-

E

G

Q

I

I

P

P

D
|
D

G

G

I

M

R

C

T

I

D

D

A

A

N

E

G

G

N

K

L

L

W

W

S

V

S

A

A

C

G
x
Y

D

N

G

G

-

G

-

R

-

V

V

I

H

R

C

L

F

D

D

P

T

E

A

T

G

G

K

K

L

R

I

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

Q

D

T

K

V

T

A

T

N

S

L

C

T

C

F

F

G

G

P

K

R

D

R

Y

N

S

R

E

L

M

F

Y

I

V

A

T

A

C

T

A

R

R

binding beta-D-glucopyranose: E16 (= E35), G66 (= G84), W80 (≠ E100), E81 (≠ V101), D92 (≠ F112), E93 (= E113), R99 (≠ S119), N101 (= N121), E119 (= E145), Y133 (= Y159), K143 (≠ T173), N152 (= N179), S168 (= S195), D202 (= D233), Y217 (≠ G248)
binding calcium ion: E16 (= E35), N152 (= N179), D202 (= D233)

4gn8A Mouse smp30/gnl-1,5-ag complex (see paper)
28% identity, 85% coverage: 32:289/304 of query aligns to 13:262/297 of 4gn8A

query
sites
4gn8A

R

R

W

C

A

G

E
|
E

G

S

P

P

V

V

W

W

F

E

N

E

D

A

A

S

N

Q

Q

S

L

L

W

F

S

V

D

D

I

I

P

P

N

S

Q

K

R

I

I

I

L

C

R

R

W

W

-

D

T

T

P

V

E

S

G

N

G

Q

V

V

S

Q

V

R

Y

V

R

A

Q

V

P

D

S

A

N

P

F

V

T

S

N

S

G

V

H

A

T

L

R

R

D
x
Q

R

L

R

G

G

G

R

Y

L

V

I

A

S

T

C

I

E

-

H

-

G

G

T

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

V

E

D

N

G

Q

S

S

I

V

T

F

V

V

L

L

A

A

D

M

R

V

F

D

E

E

G

D

R

K

L

N

N

N

S
x
R

P

F

N
|
N

D

D

V

G

V

K

V

V

K

D

S

P

D
x
A

G
|
G

T

R

I

-

W

Y

F

F

T

A

D

-

P

-

T

-

Y

-

G

G

I

T

M

M

S

A

D

E

Y

-

E
|
E

G

T

Y

A

H
x
P

A

A

D

V

P

L

E

E

Q

R

P

H

T

Q

R

G

N

S

V

L

Y

Y

R

S

L

L

D

F

P

P

E

D

T

-

G

-

E

-

L

-

S

H

A

S

V

V

V

K

T

K

D

Y

F

F

T

D

Q

Q

-

V

-

D

-

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

K

K

I

I

L

F

Y
|
Y

V

Y

A

I

D

D

S

S

S

-

A

-

S

-

H

-

D

-

R

-

L

L

P

S

R

Y

H

T

I

V

R

D

A
|
A

F

F

D

D

L

Y

-
x
D

T

L

D
x
Q

G

T

G

G

R

Q

L

I

A

S

N

N

G

R

R
|
R

V

I

F
x
V

C

Y

V

K

I

M

D

E

K

K

-

D

-

E

G

Q

I

I

P

P

D
|
D

G

G

I

M

R

C

T

I

D

D

A

A

N

E

G

G

N

K

L

L

W

W

S

V

S

A

A

C

G
x
Y

D

N

G

G

-

G

-

R

-

V

V

I

H

R

C

L

F

D

D
x
P

T

E

A

T

G

G

K

K

L

R

I

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

Q

D

T

K

V

T

A

T

N

S

L

C

T

C

F

F

G

G

P

K

R

D

R

Y

N

S

R

E

L

M

F

Y

I

V

A

T

A

C

T

A

R

R

binding 1,5-anhydro-D-glucitol: Q63 (≠ D81), R99 (≠ S119), N101 (= N121), A108 (≠ D128), G109 (= G129), E119 (= E145), P122 (≠ H148), N152 (= N179), Y164 (= Y191), A175 (= A209), D179 (vs. gap), Q181 (≠ D214), R189 (= R222), V191 (≠ F224), D202 (= D233), Y217 (≠ G248), P227 (≠ D255)
binding calcium ion: E16 (= E35), N152 (= N179), D202 (= D233)

Sites not aligning to the query:

binding 1,5-anhydro-D-glucitol: 293, 294

4gn7A Mouse smp30/gnl (see paper)
28% identity, 85% coverage: 32:289/304 of query aligns to 13:262/297 of 4gn7A

query
sites
4gn7A

R

R

W

C

A

G

E
|
E

G

S

P

P

V

V

W

W

F

E

N

E

D

A

A

S

N

Q

Q

S

L

L

W

F

S

V

D

D

I

I

P

P

N

S

Q

K

R

I

I

I

L

C

R

R

W

W

-

D

T

T

P

V

E

S

G

N

G

Q

V

V

S

Q

V

R

Y

V

R

A

Q

V

P

D

S

A

N

P

F

V

T

S

N

S

G

V

H

A

T

L

R

R

D

Q

R

L

R

G

G

G

R

Y

L

V

I

A

S

T

C

I

E

-

H

-

G

G

T

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

V

E

D

N

G

Q

S

S

I

V

T

F

V

V

L

L

A

A

D

M

R

V

F

D

E

E

G

D

R

K

L

N

N

N

S

R

P

F

N

N

D

D

V

G

V

K

V

V

K

D

S

P

D

A

G

G

T

R

I

-

W

Y

F

F

T

A

D

-

P

-

T

-

Y

-

G

G

I

T

M

M

S

A

D

E

Y

-

E

E

G

T

Y

A

H

P

A

A

D

V

P

L

E

E

Q

R

P

H

T

Q

R

G

N

S

V

L

Y

Y

R

S

L

L

D

F

P

P

E

D

T

-

G

-

E

-

L

-

S

H

A

S

V

V

V

K

T

K

D

Y

F

F

T

D

Q

Q

-

V

-

D

-

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

K

K

I

I

L

F

Y

Y

V

Y

A

I

D

D

S

S

S

-

A

-

S

-

H

-

D

-

R

-

L

L

P

S

R

Y

H

T

I

V

R

D

A

A

F

F

D

D

L

Y

-

D

T

L

D

Q

G

T

G

G

R

Q

L

I

A

S

N

N

G

R

R

R

V

I

F

V

C

Y

V

K

I

M

D

E

K

K

-

D

-

E

G

Q

I

I

P

P

D
|
D

G

G

I

M

R

C

T

I

D

D

A

A

N

E

G

G

N

K

L

L

W

W

S

V

S

A

A

C

G

Y

D

N

G

G

-

G

-

R

-

V

V

I

H

R

C

L

F

D

D

P

T

E

A

T

G

G

K

K

L

R

I

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

Q

D

T

K

V

T

A

T

N

S

L

C

T

C

F

F

G

G

P

K

R

D

R

Y

N

S

R

E

L

M

F

Y

I

V

A

T

A

C

T

A

R

R

binding calcium ion: E16 (= E35), N152 (= N179), D202 (= D233)

8dk0A Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound (s)gamma- valerolactone (see paper)
23% identity, 85% coverage: 27:285/304 of query aligns to 6:268/293 of 8dk0A

query
sites
8dk0A

L

L

Y

A

S

T

G

D

C

L

R

A

W
x
F

A

P

E
|
E

G

G

P

P

V

V

W

V

F

M

N

P

D

D

A

G

N

S

Q

V

L

V

L

L

W

-

S

V

D

E

I

I

P

R

N

A

Q

Q

R

Q

I

L

L

T

R

R

W

V

T

W

P

P

E

D

G

G

G

R

V

K

S

E

V

V

Y

V

R

A

Q

K

P

V

S

P

N

G

F

G

T

P

N

N

G

G

H

A

T

A

R

L

D

G

R

P

R

D

G

G

R

K

L

M

I

Y

S

I

C

C

E

N

H

N

G

G

T

G

-
x
F

-

G

-

W

-

M

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

R

G

R

S

V

I

T

Q

R

R

T

V

E

D

V

L

D

Q

-

S

G

G

S

E

I

V

T

E

V

T

L

L

A

F

D

D

R

K

F

C

E

G

G

E

R

H

R

P

L

L

N

K

S

G

P

P

N
|
N

D

D

V

L

V

V

V

F

K

D

S

K

D

H

G

G

T

G

I

L

W

W

F

F

T

T

D

D

P

-

T

-

Y

-

G

-

I

-

M

-

S

-

D

-

Y

-

E

L

G

G

Y

K

H

R

A

R

D

A

P

R

E

D

Q

M

P

D

T

V

R

G

N

A

V

A

Y

Y

R

Y

L

I

D

K

P

P

E

G

T

M

G

T

E

E

L

I

S

T

A

E

V

Q

V

V

T

F

D

G

F

T

T

L

Q

P

P

L

N
|
N

G

G

L

I

A

G

F

L

S

S

P

P

D

D

E

E

K

A

I

T

L

M

Y

Y

V

A

A

A

D

E

S

T

S

P

A

T

S

G

H

-

D

-

R

-

L

-

P

-

R

-

H

R

I

L

R

W

A

A

F

F

D

D

L

L

T

S

D

G

G

P

G

G

R

E

L

V

A

K

-

P

N

K

G

G

R

K

V

P

F

I

C

C

V

G

I

L

D

G

K

G

-

Y

G

Q

I

M

P

F

D
|
D

G
x
S

I

L

R

A

T

V

D

E

A

A

N

S

G

G

N

N

L

V

W

C

S

V

S

A

-

T

-

L

A

V

G

S

D

G

G

C

V

I

H

S

C

V

F

I

D

A

T

P

A

D

G

G

K

T

L

L

I

V

G

E

K

Q

I

V

R

P

V

T

P

G

Q

D

T

R

V

V

-

T

A
x
T

N

N

L

I

T

A

F

F

G

G

P

P

R

D

R

L

N

K

R

T

L

A

F

Y

I

I

binding calcium ion: E14 (= E35), N115 (= N121), N164 (= N179), D213 (= D233), S214 (≠ G234)
binding gamma-Valerolactone: F12 (≠ W33), E14 (= E35), F72 (vs. gap), D213 (= D233), T254 (≠ A271)

8djzA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound product (see paper)
23% identity, 85% coverage: 27:285/304 of query aligns to 6:268/293 of 8djzA

query
sites
8djzA

L

L

Y

A

S

T

G

D

C

L

R

A

W

F

A

P

E
|
E

G

G

P

P

V

V

W

V

F

M

N

P

D

D

A

G

N

S

Q

V

L

V

L

L

W

-

S

V

D

E

I

I

P

R

N

A

Q

Q

R

Q

I

L

L

T

R

R

W

V

T

W

P

P

E

D

G

G

G

R

V

K

S

E

V

V

Y

V

R

A

Q

K

P

V

S

P

N

G

F

G

T

P

N

N

G

G

H

A

T

A

R

L

D

G

R

P

R

D

G

G

R

K

L

M

I

Y

S

I

C

C

E

N

H

N

G

G

T

G

-

F

-

G

-

W

-

M

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

R

G

R

S

V

I

T

Q

R

R

T

V

E

D

V

L

D

Q

-

S

G

G

S

E

I

V

T

E

V

T

L

L

A

F

D

D

R

K

F

C

E

G

G

E

R

H

R

P

L

L

N

K

S

G

P

P

N
|
N

D

D

V

L

V

V

V

F

K

D

S

K

D

H

G

G

T

G

I

L

W

W

F

F

T

T

D

D

P

-

T

-

Y

-

G

-

I

-

M

-

S

-

D

-

Y

-

E

L

G

G

Y

K

H

R

A

R

D

A

P

R

E

D

Q

M

P

D

T

V

R

G

N

A

V

A

Y

Y

R

Y

L

I

D

K

P

P

E

G

T

M

G

T

E

E

L

I

S

T

A

E

V

Q

V

V

T

F

D

G

F

T

T

L

Q

P

P

L

N
|
N

G

G

L

I

A

G

F

L

S

S

P

P

D

D

E

E

K

A

I

T

L

M

Y

Y

V

A

A

A

D

E

S

T

S

P

A

T

S

G

H

-

D

-

R

-

L

-

P

-

R

-

H

R

I

L

R

W

A

A

F

F

D

D

L

L

T

S

D

G

G

P

G

G

R

E

L

V

A

K

-

P

N

K

G

G

R

K

V

P

F

I

C

C

V

G

I

L

D

G

K

G

-

Y

G

Q

I

M

P

F

D
|
D

G
x
S

I

L

R

A

T

V

D

E

A

A

N

S

G

G

N

N

L

V

W

C

S

V

S

A

-

T

-

L

A

V

G

S

D

G

G

C

V

I

H

S

C

V

F

I

D

A

T

P

A

D

G

G

K

T

L

L

I

V

G

E

K

Q

I

V

R

P

V

T

P

G

Q

D

T

R

V

V

-

T

A

T

N

N

L

I

T

A

F

F

G

G

P

P

R

D

R

L

N

K

R

T

L

A

F

Y

I

I

binding calcium ion: E14 (= E35), N115 (= N121), N164 (= N179), D213 (= D233), S214 (≠ G234)
binding (4R)-4-hydroxypentanoic acid: E14 (= E35), N115 (= N121), D213 (= D233)

8djfA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound tetrahedral intermediate (see paper)
23% identity, 85% coverage: 27:285/304 of query aligns to 6:268/293 of 8djfA

query
sites
8djfA

L

L

Y

A

S

T

G

D

C

L

R

A

W

F

A

P

E
|
E

G

G

P

P

V

V

W

V

F

M

N

P

D

D

A

G

N

S

Q

V

L

V

L

L

W

-

S

V

D

E

I

I

P

R

N

A

Q

Q

R

Q

I

L

L

T

R

R

W

V

T

W

P

P

E

D

G

G

G

R

V

K

S

E

V

V

Y

V

R

A

Q

K

P

V

S

P

N

G

F

G

T

P

N
|
N

G

G

H

A

T

A

R

L

D

G

R

P

R

D

G

G

R

K

L

M

I

Y

S

I

C

C

E

N

H

N

G

G

T

G

-
x
F

-

G

-

W

-

M

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

R

G

R

S

V

I

T

Q

R

R

T

V

E

D

V

L

D

Q

-

S

G

G

S

E

I

V

T

E

V

T

L

L

A

F

D

D

R

K

F

C

E

G

G

E

R

H

R

P

L

L

N

K

S

G

P

P

N
|
N

D

D

V

L

V

V

V

F

K

D

S

K

D

H

G

G

T

G

I

L

W

W

F

F

T

T

D

D

P

-

T

-

Y

-

G

-

I

-

M

-

S

-

D

-

Y

-

E

L

G

G

Y

K

H

R

A

R

D

A

P

R

E

D

Q

M

P

D

T

V

R

G

N

A

V

A

Y

Y

R

Y

L

I

D

K

P

P

E

G

T

M

G

T

E

E

L

I

S

T

A

E

V

Q

V

V

T

F

D

G

F

T

T

L

Q

P

P

L

N
|
N

G

G

L

I

A

G

F

L

S

S

P

P

D

D

E

E

K

A

I

T

L

M

Y

Y

V

A

A

A

D

E

S

T

S

P

A

T

S

G

H

-

D

-

R

-

L

-

P

-

R

-

H

R

I

L

R

W

A

A

F

F

D

D

L

L

T

S

D

G

G

P

G

G

R

E

L

V

A

K

-

P

N

K

G

G

R

K

V

P

F

I

C

C

V

G

I

L

D

G

K

G

-

Y

G

Q

I

M

P

F

D
|
D

G
x
S

I

L

R

A

T

V

D

E

A

A

N

S

G

G

N

N

L

V

W

C

S

V

S

A

-

T

-

L

A

V

G

S

D

G

G

C

V

I

H

S

C

V

F

I

D

A

T

P

A

D

G

G

K

T

L

L

I

V

G

E

K

Q

I

V

R

P

V

T

P

G

Q

D

T

R

V

V

-

T

A

T

N

N

L

I

T

A

F

F

G

G

P

P

R

D

R

L

N

K

R

T

L

A

F

Y

I

I

binding calcium ion: E14 (= E35), N115 (= N121), N164 (= N179), D213 (= D233), S214 (≠ G234)
binding (5S)-5-methyloxolane-2,2-diol: E14 (= E35), N54 (= N76), F72 (vs. gap), N115 (= N121), D213 (= D233)

7risA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound phosphate (see paper)
23% identity, 85% coverage: 27:285/304 of query aligns to 4:268/293 of 7risA

query
sites
7risA

L

L

Y

A

S

T

G

D

C

L

R

A

W

F

A

P

E
|
E

G

G

P

P

V

V

W

V

F

M

N

P

D

D

A

G

N

S

Q

V

L

V

L

L

W

-

S

V

D

E

I

I

P

R

N

A

Q

Q

R

Q

I

L

L

T

R

R

W

V

T

W

P

P

E

D

G

G

G

R

V

K

S

E

V

V

Y

V

R

A

Q

K

P

V

S

P

N

G

F

G

T

P

N

N

G

G

H

A

T

A

R

L

D

G

R

P

R

D

G

G

R

K

L

M

I

Y

S

I

C

C

E

N

H

N

G

G

T

G

-

F

-

G

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

R

G

R

S

V

I

T

Q

R

R

T

V

E

D

V

L

D

Q

-

S

G

G

S

E

I

V

T

E

V

T

L

L

A

F

D

D

R

K

F

C

E

G

G

E

R

H

R

P

L

L

N

K

S

G

P

P

N
|
N

D

D

V

L

V

V

V

F

K

D

S

K

D

H

G

G

T

G

I

L

W

W

F

F

T

T

D

D

P

-

T

-

Y

-

G

-

I

-

M

-

S

-

D

-

Y

-

E

L

G

G

Y

K

H

R

A

R

D

A

P

R

E

D

Q

M

P

D

T

V

R

G

N

A

V

A

Y

Y

R

Y

L

I

D

K

P

P

E

G

T

M

G

T

E

E

L

I

S

T

A

E

V

Q

V

V

T

F

D

G

F

T

T

L

Q

P

P

L

N
|
N

G

G

L

I

A

G

F

L

S

S

P

P

D

D

E

E

K

A

I

T

L

M

Y

Y

V

A

A

A

D

E

S

T

S

P

A

T

S

G

H

-

D

-

R

-

L

-

P

-

R

-

H

R

I

L

R

W

A

A

F

F

D

D

L

L

T

S

D

G

G

P

G

G

R

E

L

V

A

K

-

P

-

R

-

D

-

G

-

E

N

K

G

G

R

K

V

P

F

I

C

C

V

G

I

L

D

G

K

G

-

Y

G

Q

I

M

P

F

D
|
D

G
x
S

I

L

R

A

T

V

D

E

A

A

N

S

G

G

N

N

L

V

W

C

S

V

S

A

-

T

-

L

A

V

G

S

D

G

G

C

V

I

H

S

C

V

F

I

D

A

T

P

A

D

G

G

K

T

L

L

I

V

G

E

K

Q

I

V

R

P

V

T

P

G

Q

D

T

R

V

V

-

T

A
x
T

N

N

L

I

T

A

F

F

G

G

P

P

R

D

R

L

N

K

R

T

L

A

F

Y

I

I

binding calcium ion: E12 (= E35), N111 (= N121), N160 (= N179), D213 (= D233), S214 (≠ G234)
binding phosphate ion: E12 (= E35), N111 (= N121), N160 (= N179), D213 (= D233), T254 (≠ A271)

7rizA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound 2-hydroxyquinoline (see paper)
23% identity, 85% coverage: 27:285/304 of query aligns to 4:281/306 of 7rizA

query
sites
7rizA

L

L

Y

A

S

T

G

D

C

L

R

A

W

F

A

P

E
|
E

G

G

P

P

V

V

W

V

F

M

N

P

D

D

A

G

N

S

Q

V

L

V

L

L

W

-

S

V

D

E

I

I

P

R

N

A

Q

Q

R

Q

I

L

L

T

R

R

W

V

T

W

P

P

E

D

G

G

G

R

V

K

S

E

V

V

Y

V

R

A

Q

K

P

V

S

P

N

G

F

G

T

P

N
|
N

G

G

H

A

T

A

R

L

D

G

R

P

R

D

G

G

R

K

L

M

I

Y

S

I

C

C

E

N

H

N

G

G

T

G

-
x
F

-

G

-

W

-

F

-

P

-

S

-

R

-

G

-

T

-

M

-
x
P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

R

G

R

S

V

I

T

Q

R

R

T

V

E

D

V

L

D

Q

-

S

G

G

S

E

I

V

T

E

V

T

L

L

A

F

D

D

R

K

F

C

E

G

G

E

R

H

R

P

L

L

N

K

S

G

P

P

N
|
N

D

D

V

L

V

V

V

F

K

D

S

K

D

H

G

G

T

G

I

L

W

W

F

F

T

T

D

D

P

-

T

-

Y

-

G

-

I

-

M

-

S

-

D

-

Y

-

E

L

G

G

Y

K

H

R

A

R

D

A

P

R

E

D

Q

M

P

D

T

V

R

G

N

A

V

A

Y

Y

R

Y

L

I

D

K

P

P

E

G

T

M

G

T

E

E

L

I

S

T

A

E

V

Q

V

V

T

F

D

G

F

T

T

L

Q

P

P

L

N
|
N

G

G

L

I

A

G

F

L

S

S

P

P

D

D

E

E

K

A

I

T

L

M

Y

Y

V

A

A

A

D

E

S

T

S

P

A

T

S

G

H

-

D

-

R

-

L

-

P

-

R

-

H

R

I

L

R

W

A

A

F

F

D

D

L

L

T

S

D

G

G

P

G

G

R

E

L

V

A

K

N

P

G

R

R

D

V

V

F

I

C

Y

V

R

I

G

D

E

K

K

G

G

I

K

P

P

-

I

-

C

-

G

-

L

-

G

-

Y

-

Q

-

M

-

F

D
|
D

G
x
S

I

L

R

A

T

V

D

E

A

A

N

S

G

G

N

N

L

V

W

C

S

V

S

A

-

T

-

L

A

V

G

S

D

G

G

C

V

I

H

S

C

V

F

I

D

A

T

P

A

D

G

G

K

T

L

L

I

V

G

E

K

Q

I

V

R

P

V

T

P

G

Q

D

T

R

V

V

-

T

A

T

N

N

L

I

T

A

F

F

G

G

P

P

R

D

R

L

N

K

R

T

L

A

F

Y

I

I

binding calcium ion: E12 (= E35), N120 (= N121), N169 (= N179), D226 (= D233), S227 (≠ G234)
binding quinolin-2(1h)-one: E12 (= E35), N52 (= N76), F70 (vs. gap), P81 (vs. gap), N120 (= N121), D226 (= D233)

2dsoC Crystal structure of d138n mutant of drp35, a 35kda drug responsive protein from staphylococcus aureus (see paper)
28% identity, 48% coverage: 121:267/304 of query aligns to 135:269/323 of 2dsoC

query
sites
2dsoC

N
|
N

D

D

V

M

V

V

V

F

K

D

S

S

D

K

G

G

T

G

I

F

W

Y

F

F

T

T

D

D

P

-

T

-

Y

-

G

-

I

-

M

-

S

-

D

-

Y

F

E

R

G

G

Y

Y

H

S

A

T

D

N

P

P

E

-

Q

-

P

-

T

L

R

G

N

G

V

V

Y

Y

R

Y

L

V

D

S

P

P

E

D

T

F

G

R

E

T

L

V

S

T

A

P

V

I

T

Q

D

N

F

I

T

S

Q

V

P

A

N
|
N

G

G

L

I

A

A

F

L

S

S

P

T

D

D

E

E

K

K

I

V

L

L

Y

W

V

V

A

T

D

E

S

T

A

A

S

N

H

R

D

L

D

H

R

R

L

I

P

A

R

L

H

E

I

D

R

D

A

G

F

V

D

T

L

I

T

Q

D

P

G

F

G

G

R

-

L

-

A

A

N

T

G

I

R

P

V

Y

F

Y

C

F

V

T

I

G

D

H

K

E

G

G

I

-

P

P

D
|
D

G
x
S

I

C

R

C

T

I

D

D

A

S

N

D

G

D

N

N

L

L

W

Y

S

V

S

A

A

M

-

Y

G

G

D

Q

G

G

-

R

V

V

H

L

C

V

F

F

D

N

T

K

A

R

G

G

K

Y

L

P

I

I

G

G

K

Q

I

I

R

L

V

I

P

P

binding calcium ion: N135 (= N121), N182 (= N179), D233 (= D233), S234 (≠ G234)

Sites not aligning to the query:

binding calcium ion: 45, 107, 108, 109, 127, 130, 131, 132

Q99QV3 Lactonase drp35; EC 3.1.1.- from Staphylococcus aureus (strain Mu50 / ATCC 700699) (see paper)
28% identity, 48% coverage: 121:267/304 of query aligns to 137:271/324 of Q99QV3

query
sites
Q99QV3

N
x
D

D
|
D

V

M

V

V

V

F

K

D

S

S

D

K

G

G

T

G

I

F

W

Y

F

F

T

T

D
|
D

P

-

T

-

Y

-

G

-

I

-

M

-

S

-

D

-

Y

F

E

R

G

G

Y

Y

H

S

A

T

D

N

P

P

E

-

Q

-

P

-

T

L

R

G

N

G

V

V

Y

Y

R

Y

L

V

D

S

P

P

E

D

T

F

G

R

E

T

L

V

S

T

A

P

V

I

T

Q

D

N

F

I

T

S

Q

V

P

A

N
|
N

G
|
G

L

I

A

A

F

L

S

S

P

T

D

D

E

E

K

K

I

V

L

L

Y

W

V

V

A

T

D

E

S

T

A

A

S

N

H

R

D

L

D

H

R

R

L

I

P

A

R

L

H

E

I

D

R

D

A

G

F

V

D

T

L

I

T

Q

D

P

G

F

G

G

R

-

L

-

A

A

N

T

G

I

R

P

V

Y

F

Y

C

F

V

T

I

G

D

H

K

E

G

G

I

-

P

P

D
|
D

G
x
S

I
x
C

R

C

T

I

D

D

A

S

N

D

G

D

N

N

L

L

W

Y

S

V

S

A

A

M

-

Y

G

G

D

Q

G

G

-

R

V

V

H

L

C

V

F

F

D

N

T

K

A

R

G

G

K

Y

L

P

I

I

G

G

K

Q

I

I

R

L

V

I

P

P

D137 (≠ N121) mutation to A: Loss of activity.; mutation to N: Loss of activity but still able to bind calcium ion.; mutation to S: Loss of activity.
D138 (= D122) mutation to A: Decrease in lactonase activity.
D151 (= D135) mutation to A: Decrease in lactonase activity.
N184 (= N179) mutation to A: Loss of activity.; mutation to D: Decrease in lactonase activity.; mutation to S: Loss of activity.
G185 (= G180) mutation to A: Decrease in lactonase activity.
D235 (= D233) mutation to A: Loss of activity.; mutation to N: Loss of activity due to inability to bind calcium ion.; mutation to S: Loss of activity.
S236 (≠ G234) mutation to A: Decrease in lactonase activity.
C237 (≠ I235) mutation to A: Increase in lactonase activity.

Sites not aligning to the query:

47 E→A: Loss of activity.; E→Q: Loss of activity due to inability to bind calcium ion.; E→S: Loss of activity.
63 F→A: 2.5-fold increase in lactonase activity.
92 K→A: Increase in lactonase activity.
280 R→A: Increase in lactonase activity.
301 E→A: Increase in lactonase activity.

Q9M1B4 Protein STRICTOSIDINE SYNTHASE-LIKE 13; AtSSL13; Protein LESS ADHERENT POLLEN 3; Strictosidine synthase 11; AtSS11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 47% coverage: 103:246/304 of query aligns to 181:315/403 of Q9M1B4

query
sites
Q9M1B4

G

G

S

I

I

A

T

T

V

P

L

L

A

A

D

T

R

H

F

V

E

E

G

G

R

K

R

P

L

I

N

L

S

F

P

A

N

N

D

D

V

L

V

D

V

I

K

H

S

R

D

N

G

G

T

S

I

I

W

F

F

F

T

T

D

D

P

T

T

S

Y

K

G

R

I

Y

M

D

S

R

D

A

Y

N

E

H

G

F

Y

F

H

I

A

L

D

L

P

E

E

G

Q

E

P

S

T

T

R

G

N

R

V

L

Y

L

R

R

L

Y

D

D

P

P

E

P

T

T

G

K

E

T

L

T

S

H

A

I

V

V

V

L

T

E

D

G

F

L

T

A

Q

F

P

P

N

N

G

G

L

I

A

Q

F

L

S

S

P

K

D

D

E

Q

K

S

I

F

L

L

Y

L

V

F

A

T

D

E

S

T

A

-

S

-

H

-

D

N

D

C

R

R

L

L

P

V

R

K

H

Y

I

-

R

-

A

-

F

-

D

-

L

W

T

L

D

E

G

G

G

P

R

K

L

M

A

-

N

-

G

G

R

E

V

V

F

E

C

V

V

V

I

A

D

D

-

L

K

P

G

G

I

F

P

P

D

D

G

N

I

V

R

R

T

I

D

N

A

E

N

E

G

G

N

Q

L

F

W

W

S

V

S

A

Sites not aligning to the query:

89 E→K: In lap3-1; abnormal pollen exine.

Query Sequence

>SMa0196 FitnessBrowser__Smeli:SMa0196
MAEASIYEIHDPRFRQMIVTSAGLDELYSGCRWAEGPVWFNDANQLLWSDIPNQRILRWT
PEGGVSVYRQPSNFTNGHTRDRRGRLISCEHGTRRVTRTEVDGSITVLADRFEGRRLNSP
NDVVVKSDGTIWFTDPTYGIMSDYEGYHADPEQPTRNVYRLDPETGELSAVVTDFTQPNG
LAFSPDEKILYVADSSASHDDRLPRHIRAFDLTDGGRLANGRVFCVIDKGIPDGIRTDAN
GNLWSSAGDGVHCFDTAGKLIGKIRVPQTVANLTFGGPRRNRLFIAATRSLYSVYVAVTG
SQVP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory