SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMa0478 FitnessBrowser__Smeli:SMa0478 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P33160 Formate dehydrogenase; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Pseudomonas sp. (strain 101) (Achromobacter parvulus T1) (see 3 papers)
79% identity, 99% coverage: 3:400/401 of query aligns to 1:398/401 of P33160

query
sites
P33160

M
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M

A

A

K

K

V

V

A

L

C

C

V

V

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L

Y

Y

D

D

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D

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E

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S

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F

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P

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A

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T

A

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E

E

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R

I

I

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K

A

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N

L

L

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K

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L

A

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I

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D

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D

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N

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x
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L
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L

H
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H

P
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E
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E

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T

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K

L

M

F

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K

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R

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G

A

A

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Y

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T

A

A

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R

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G

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N

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R

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D

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M1 (= M3) modified: Initiator methionine, Removed
I123 (= I125) binding
N147 (= N149) binding
S148 (= S150) binding
RI 202:203 (= RI 204:205) binding
D222 (= D224) binding
C256 (≠ A258) mutation C->S,M: High resistance to inactivation by Hg(2+), Increased stability at 25 degree Celsius and decreased thermostability at 45 degree Celsius.
PLHPE 257:261 (= PLHPE 259:263) binding
T283 (= T285) binding
D309 (= D311) binding
HISG 333:336 (= HISG 335:338) binding
S381 (= S383) binding

3wr5A Structural basis on the efficient co2 reduction of acidophilic formate dehydrogenase
78% identity, 99% coverage: 3:399/401 of query aligns to 5:401/401 of 3wr5A

query
sites
3wr5A

M

M

A

A

K

K

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I

A

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C

C

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V

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L

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Y

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D

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D

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G

Y

Y

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A

T

Y

Y

A

A

R

R

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D

G

D

L

L

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P

T

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L

I

E

D

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Y

Y

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Q

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T

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L

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P

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T

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P

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K

A

A

I

I

D

D

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F

E

T

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P

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G

A

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L

L

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G

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S

G

G

E

E

L

L

G

G

L

L

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K

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Y

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L

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E

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A

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G

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H

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F

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T

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D

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K

D

D

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F

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E

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A

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A

A

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N

L

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K

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L

A

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G

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E

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N

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S

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E

H

H

V

V

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M

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x
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F

active site: N151 (= N149), R289 (= R287), D313 (= D311), Q318 (= Q316), H337 (= H335)
binding nicotinamide-adenine-dinucleotide: N151 (= N149), V155 (= V153), A203 (≠ G201), G205 (= G203), R206 (= R204), I207 (= I205), D226 (= D224), R227 (= R225), V260 (≠ A258), P261 (= P259), T287 (= T285), A288 (= A286), R289 (= R287), D313 (= D311), H337 (= H335), S339 (= S337), G340 (= G338), S385 (= S383)

6t9wB Crystal structure of formate dehydrogenase fdh2 d222a/q223r enzyme from granulicella mallensis mp5actx8 in complex with NADP and azide.
82% identity, 95% coverage: 4:385/401 of query aligns to 1:382/383 of 6t9wB

query
sites
6t9wB

A

A

K

K

V

I

A

L

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C

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V

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L

Y

Y

D

D

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P

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A

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Y

A

A

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D

A

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D

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Y

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A

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T

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K

A

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I

I

D

D

F

F

E

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P

G

G

A

E

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S

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V

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S

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G

E

E

L

L

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G

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D

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K

D

E

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E

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D

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S

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F

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R

E

E

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D

D

A

A

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E

I

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S

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Q

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F
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F

W

W

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P

A

A

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A

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R

I

I

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A

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K

A

A

A

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R

K

L

L

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L

L

A

A

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T

A

A

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G

I
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I

G

G

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S

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D

H

H

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V

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D

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L

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E

A

A

I

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G

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A

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E

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N

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S

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E

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H

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V

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M

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Y

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I

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S

Y

Y

Q

Q

W

W

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V

V

I

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K

G

G

W

W

N

N

V

I

A

A

D

D

C

C

V

V

A

E

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Y

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D

D

I

L

E

E

G

A

M

M

D

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I

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G

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T

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G
x
A

A

A

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G

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R

I
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I

G

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T

L

A

A

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L

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K

K

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F

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D

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x
F

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R

H

H

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R

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L

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D

E

E

S

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A

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N

E

E

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H

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A

E

E

D

M

M

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V

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V

V

C

C

D

D

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T

I

I

N

N

A

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P

L

L

H
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H

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E

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T
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T

E

L

N

D

L

L

F

F

N

N

E

D

A

E

M

L

I

I

G

S

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K

M

M

K

K

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R

G

G

A

A

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Y

L

L

V

V

N

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T
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T

A
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A

R
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R

G

G

K

K

I

I

C

C

N

N

R

R

D

D

A

A

V

V

A

V

R

R

A

A

L

L

E

E

S

S

G

G

Q

Q

L

L

A

A

G

G

Y

Y

A

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

K

K

D

D

H

H

P

P

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W

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R

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M

M

P

P

H

H

G

G

M

M

T

T

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H
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H

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S

G
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G

S

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S

S

L

L

S

S

A

A

Q

Q

A

A

R

R

Y

Y

A

A

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G

T

T

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R

E

E

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I

L

L

E

E

C

C

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F

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E

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I

R

R

E

E

Y

Y

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L

I

I

V

V

S

D

G

G

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K

L

L

A

A

G

G

A

T

G

G

A

A

H

H

S
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S

Y

Y

S

T

binding azide ion: P97 (= P100), F98 (= F101), G121 (= G124), I122 (= I125), H332 (= H335)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I122 (= I125), N146 (= N149), S147 (= S150), V150 (= V153), A198 (≠ G201), G200 (= G203), R201 (= R204), I202 (= I205), F220 (≠ T223), R222 (= R225), P256 (= P259), H258 (= H261), T261 (= T264), T282 (= T285), A283 (= A286), R284 (= R287), D308 (= D311), H332 (= H335), S334 (= S337), G335 (= G338), S380 (= S383)

2nadA High resolution structures of holo and apo formate dehydrogenase (see paper)
79% identity, 98% coverage: 4:394/401 of query aligns to 1:391/391 of 2nadA

query
sites
2nadA

A

A

K

K

V

V

A

L

C

C

V

V

L

L

Y

Y

D

D

P

P

V

V

D

D

G

G

Y

Y

P

P

T

K

A

T

Y

Y

A

A

R

R

D

D

G

D

L

L

P

P

T

K

L

I

E

D

R

H

Y

Y

P

P

G

G

Q

Q

T

T

L

L

P

P

T

T

P

P

K

K

A

A

I

I

D

D

F

F

E

T

P

P

G

G

A

Q

L

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

K

K

F

Y

L

L

E

E

G

S

Q

N

G

G

H

H

T

T

L

L

V

V

T

T

S

S

D

D

K

K

D

D

G

G

P

P

D

D

S

S

V

V

F

F

E

E

R

R

E

E

L

L

V

V

D

D

A

A

E

D

I

V

V

V

I

I

S

S

Q

Q

P
|
P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

A

P

E

E

R

R

I

I

V

A

K

K

A

A

A

K

R

N

L

L

K

K

L

L

A

A

I

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

Q

A

S

A

A

I

I

D

D

R

R

G

N

I

V

T

T

V

V

A

A

E

E

V

V

T

T

Y

Y

C

C

N
|
N

S

S

I

I

S

S

V
|
V

S

A

E

E

H

H

V

V

M

M

I

I

L

L

S

S

L

L

A

V

R

R

N

N

Y

Y

I

L

P

P

S

S

Y

H

Q

E

W

W

V

A

V

R

K

K

G

G

W

W

N

N

V

I

A

A

D

D

C

C

V

V

A

S

R

H

S

A

Y

Y

D

D

I

L

E

E

G

A

M

M

D

H

I

V

G

G

T

T

V

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

T

L

A

A

V

V

L

L

R

R

L

L

K

A

P

P

F

F

D

D

V

V

K

H

L

L

H

H

Y

Y

T

T

D
|
D

R

R

H

H

R

R

L

L

P

P

D

E

E

S

V

V

A

E

K

K

E

E

L

L

G

N

V

L

T

T

F

W

H

H

Q

A

T

T

A

R

A

E

E

D

M

M

V

Y

P

P

V

V

C

C

D

D

V

V

T

T

I

L

N

N

A

C

P
|
P

L

L

H

H

P

P

E
|
E

T
|
T

E

E

N

H

L

M

F

I

N

N

E

D

A

E

M

T

I

L

G

K

K

L

M

F

K

K

R

R

G

G

A

A

Y

Y

L

I

V

V

N

N

T
|
T

A
|
A

R
|
R

G

G

K

K

I

L

C

C

N

D

R

R

D

D

A

A

V

V

A

A

R

R

A

A

L

L

E

E

S

S

G

G

Q

R

L

L

A

A

G

G

Y

Y

A

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q
|
Q

P

P

A

A

P

P

K

K

D

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

H

Y

H

N

G

G

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

S

T

L

L

S

T

A

A

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

W

F

F

F

E

E

G

G

R

R

P

P

I

I

R

R

E

D

E

E

Y

Y

L

L

I

I

V

V

S

Q

G

G

K

A

L

L

A

A

G

G

A

T

G

G

A

A

H

H

S
|
S

Y

Y

S

S

A

K

G

G

D

N

A

A

T

T

R

G

G

G

S

S

E

E

active site: N146 (= N149), R284 (= R287), D308 (= D311), Q313 (= Q316), H332 (= H335)
binding azide ion: P97 (= P100), F98 (= F101), I122 (= I125), N146 (= N149), R284 (= R287)
binding nicotinamide-adenine-dinucleotide: I122 (= I125), N146 (= N149), V150 (= V153), A198 (≠ G201), G200 (= G203), R201 (= R204), I202 (= I205), D221 (= D224), P256 (= P259), E260 (= E263), T261 (= T264), T282 (= T285), A283 (= A286), R284 (= R287), D308 (= D311), H332 (= H335), S334 (= S337), G335 (= G338), S380 (= S383)

2gsdA NAD-dependent formate dehydrogenase from bacterium moraxella sp.C2 in complex with NAD and azide (see paper)
76% identity, 99% coverage: 4:400/401 of query aligns to 1:397/399 of 2gsdA

query
sites
2gsdA

A

A

K

K

V

V

A

V

C

C

V

V

L

L

Y

Y

D

D

P

P

V

I

D

N

G

G

Y

Y

P

P

T

T

A

S

Y

Y

A

A

R

R

D

D

G

D

L

L

P

P

T

R

L

I

E

D

R

K

Y

Y

P

P

G

D

G

G

Q

Q

T

T

L

L

P

P

T

T

P

P

K

K

A

A

I

I

D

D

F

F

E

T

P

P

G

G

A

A

L

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

K

K

F

Y

L

L

E

E

G

S

Q

Q

G

G

H

H

T

E

L

L

V

V

T

T

S

S

D

S

K

K

D

D

G

G

P

P

D

D

S

S

V

E

F

L

E

E

R

K

E

H

L

L

V

H

D

D

A

A

E

E

I

V

V

I

I

I

S

S

Q

Q

P
|
P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

A

A

E

E

R

R

I

I

V

A

K

K

A

A

A

P

R

K

L

L

K

K

L

L

A

A

I

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

Q

A

A

I

I

D

D

R

N

G

N

I

I

T

T

V

V

A

A

E

E

V

V

T

T

Y

Y

C

C

N
|
N

S

S

I

N

S

S

V
|
V

S

A

E

E

H

H

V

V

M

M

I

V

L

L

S

G

L

L

A

V

R

R

N

N

Y

Y

I

I

P

P

S

S

Y

H

Q

D

W

W

V

A

V

R

K

N

G

G

W

W

N

N

V

I

A

A

D

D

C

C

V

V

A

A

R

R

S

S

Y

Y

D

D

I

V

E

E

G

G

M

M

D

H

I

V

G

G

T

T

V

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

T

L

A

R

V

V

L

L

R

R

R

L

L

L

K

A

P

P

F

F

D

D

V

M

K

H

L

L

H

H

Y

Y

T

T

D
|
D

R
|
R

H

H

R

R

L

L

P

P

D

E

E

A

V

V

A

E

K

K

E

E

L

L

G

N

V

L

T

T

F

W

H

H

Q

A

T

T

A

R

A

E

E

D

M

M

V

Y

P

G

V

A

C

C

D

D

V

V

T

T

I

L

N

N

A

C

P
|
P

L

L

H
|
H

P

P

E

E

T
|
T

E

E

N

H

L

M

F

I

N

N

E

D

A

E

M

T

I

L

G

K

K

L

M

F

K

K

R

R

G

G

A

A

Y

Y

L

L

V

V

N

N

T
|
T

A
|
A

R
|
R

G

G

K

K

I

L

C

C

N

D

R

R

D

D

A

A

V

I

A

V

R

R

A

A

L

L

E

E

S

S

G

G

Q

R

L

L

A

A

G

G

Y

Y

A

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q
|
Q

P

P

A

A

P

P

K

N

D

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

H

H

H

N

G

G

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

S

T

S

S

L

L

S

S

A

A

Q

Q

A

T

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

W

Y

F

F

E

E

G

G

R

R

P

P

I

I

R

R

E

D

E

E

Y

Y

L

L

I

I

V

V

S

Q

G

G

K

G

L

L

A

A

G

G

A

V

G

G

A

A

H

H

S
|
S

Y

Y

S

S

A

K

G

G

D

N

A

A

T

T

R

G

G

G

S

S

E

E

A

A

H

K

F

Y

K

E

active site: N146 (= N149), R284 (= R287), D308 (= D311), Q313 (= Q316), H332 (= H335)
binding azide ion: P97 (= P100), F98 (= F101), I122 (= I125), R284 (= R287), H332 (= H335)
binding nicotinamide-adenine-dinucleotide: I122 (= I125), N146 (= N149), V150 (= V153), A198 (≠ G201), G200 (= G203), R201 (= R204), I202 (= I205), D221 (= D224), R222 (= R225), P256 (= P259), H258 (= H261), T261 (= T264), T282 (= T285), A283 (= A286), R284 (= R287), D308 (= D311), H332 (= H335), S334 (= S337), G335 (= G338), S380 (= S383)

8bxxAA Formate dehydrogenase
82% identity, 92% coverage: 4:373/401 of query aligns to 1:370/370 of 8bxxAA

query
sites
8bxxAA

A

A

K

K

V

I

A

L

C

C

V

V

L

L

Y

Y

D

D

P

P

V

I

D

T

G

G

Y

Y

P

P

T

K

A

S

Y

Y

A

A

R

R

D

A

G

D

L

V

P

P

T

K

L

I

E

D

R

H

Y

Y

P

P

G

G

Q

Q

T

T

L

A

P

P

T

T

P

P

K

K

A

Q

I

I

D

D

F

F

E

T

P

P

G

G

A

E

L

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

K

K

F

Y

L

L

E

E

G

G

Q

L

G

G

H

H

T

T

L

L

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

E

D

D

S

S

V

V

F

F

E

E

R

R

E

E

L

L

V

P

D

D

A

A

E

E

I

I

V

V

I

I

S

S

Q

Q

P
|
P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

A

P

E

E

R

R

I

I

V

A

K

K

A

A

A

K

R

K

L

L

K

K

L

L

A

A

I

V

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

E

A

A

I

I

D

K

R

N

G

G

I

I

T

T

V

V

A

A

E

E

V

V

T

T

Y

Y

C

S

N
|
N

S

S

I

I

S

S

V
|
V

S

S

E

E

H

H

V

V

M

M

I

I

L

L

S

S

L

L

A

V

R

R

N

N

Y

Y

I

I

P

P

S

S

Y

Y

Q

Q

W

W

V

V

V

I

K

K

G

G

W

W

N

N

V

I

A

A

D

D

C

C

V

V

A

E

R

R

S

S

Y

Y

D

D

I

L

E

E

G

A

M

M

D

H

I

V

G

G

T

T

V

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

T

L

A

A

V

V

L

L

R

K

R

R

L

L

K

K

P

P

F

F

D

D

V

V

K

K

L

L

H

H

Y

Y

T
x
F

D
|
D

R
x
Q

H

H

R

R

L

L

P

P

D

E

E

S

V

V

A

E

K

N

E

E

L

L

G

G

V

L

T

T

F

Y

H

H

Q

P

T

S

A

V

A

E

E

D

M

M

V

V

P

K

V

V

C

C

D

D

V

V

T

T

I

I

N

N

A

A

P
|
P

L

L

H
|
H

P

P

E

G

T

T

E

L

N

D

L

L

F

F

N

N

E

D

A

E

M

L

I

I

G

S

K

K

M

M

K

K

R

R

G

G

A

A

Y

Y

L

L

V

V

N

N

T
|
T

A
|
A

R
|
R

G

G

K

K

I

I

C

C

N

N

R

R

D

D

A

A

V

V

A

V

R

R

A

A

L

L

E

E

S

S

G

G

Q

Q

L

L

A

A

G

G

Y

Y

A

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

K

K

D

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

H

H

G

G

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

S

T

S

S

L

L

S

S

A

A

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

W

W

F

F

E

E

G

E

R

R

P

P

I

I

R

R

E

E

Y

Y

L

L

I

I

V

V

S

D

G

G

binding azide ion: P97 (= P100), F98 (= F101), I122 (= I125), R284 (= R287), H332 (= H335)
binding nicotinamide-adenine-dinucleotide: I122 (= I125), N146 (= N149), V150 (= V153), A198 (≠ G201), G200 (= G203), R201 (= R204), I202 (= I205), F220 (≠ T223), D221 (= D224), Q222 (≠ R225), P256 (= P259), H258 (= H261), T282 (= T285), A283 (= A286), R284 (= R287), D308 (= D311), H332 (= H335), S334 (= S337), G335 (= G338)

6jujA Crystal structure of formate dehydrogenase mutant v198i/c256i/p260s/e261p/s381n/s383f from pseudomonas sp. 101in complex with non-natural cofactor nicotinamide cytosine dinucleotide (see paper)
78% identity, 95% coverage: 4:384/401 of query aligns to 1:381/381 of 6jujA

query
sites
6jujA

A

A

K

K

V

V

A

L

C

C

V

V

L

L

Y

Y

D

D

P

P

V

V

D

D

G

G

Y

Y

P

P

T

K

A

T

Y

Y

A

A

R

R

D

D

G

D

L

L

P

P

T

K

L

I

E

D

R

H

Y

Y

P

P

G

G

Q

Q

T

T

L

L

P

P

T

T

P

P

K

K

A

A

I

I

D

D

F

F

E

T

P

P

G

G

A

Q

L

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

K

K

F

Y

L

L

E

E

G

S

Q

N

G

G

H

H

T

T

L

L

V

V

T

T

S

S

D

D

K

K

D

D

G

G

P

P

D

D

S

S

V

V

F

F

E

E

R

R

E

E

L

L

V

V

D

D

A

A

E

D

I

V

V

V

I

I

S

S

Q

Q

P

P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

A

P

E

E

R

R

I

I

V

A

K

K

A

A

A

K

R

N

L

L

K

K

L

L

A

A

I

L

T

T

A

A

G

G

I

I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

Q

A

S

A

A

I

I

D

D

R

R

G

N

I

V

T

T

V

V

A

A

E

E

V

V

T

T

Y

Y

C

C

N
|
N

S

S

I

I

S

S

V
|
V

S

A

E

E

H

H

V

V

M

M

I

I

L

L

S

S

L

L

A

V

R

R

N

N

Y

Y

I

L

P

P

S

S

Y

H

Q

E

W

W

V

A

V

R

K

K

G

G

W

W

N

N

V

I

A

A

D

D

C

C

V

V

A

S

R

H

S

A

Y

Y

D

D

I

L

E

E

G

A

M

M

D

H

I

V

G

G

T

T

V

I

G

A

A

A

G
|
G

R

R

I
|
I

G

G

T

L

A

A

V

V

L

L

R

R

L

L

K

A

P

P

F

F

D

D

V

V

K

H

L

L

H

H

Y

Y

T

T

D
|
D

R
|
R

H

H

R

R

L

L

P

P

D

E

E

S

V

V

A

E

K

K

E

E

L

L

G

N

V

L

T

T

F

W

H

H

Q

A

T

T

A

R

A

E

E

D

M

M

V

Y

P

P

V

V

C

C

D

D

V

V

T

T

I

L

N

N

A
x
I

P
|
P

L

L

H
|
H

P

S

E

P

T

T

E

E

N

H

L

M

F

I

N

N

E

D

A

E

M

T

I

L

G

K

K

L

M

F

K

K

R

R

G

G

A

A

Y

Y

L

I

V

V

N

N

T
|
T

A
|
A

R

R

G

G

K

K

I

L

C

C

N

D

R

R

D

D

A

A

V

V

A

A

R

R

A

A

L

L

E

E

S

S

G

G

Q

R

L

L

A

A

G

G

Y

Y

A

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

K

K

D

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

H

Y

H

N

G

G

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

S

T

L

L

S

T

A

A

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

W

F

F

F

E

E

G

G

R

R

P

P

I

I

R

R

E

D

E

E

Y

Y

L

L

I

I

V

V

S

Q

G

G

K

A

L

L

A

A

G

G

A

T

G

G

A

A

H

H

S

N

Y

Y

binding [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: F98 (= F101), N146 (= N149), V150 (= V153), G200 (= G203), I202 (= I205), D221 (= D224), R222 (= R225), I255 (≠ A258), P256 (= P259), H258 (= H261), T282 (= T285), A283 (= A286), D308 (= D311), H332 (= H335), S334 (= S337), G335 (= G338)

4xyeA Granulicella m. Formate dehydrogenase (fdh) in complex with NAD(+) (see paper)
73% identity, 96% coverage: 4:387/401 of query aligns to 1:384/384 of 4xyeA

query
sites
4xyeA

A

A

K

K

V

V

A

L

C

C

V

V

L

L

Y

Y

D

D

P

P

V

T

D

S

G

G

Y

Y

P

P

T

P

A

L

Y

Y

A

A

R

R

D

N

G

A

L

I

P

P

T

K

L

I

E

E

R

R

Y

Y

P

P

G

D

G

G

Q

Q

T

T

L

V

P

P

T

N

P

P

K

K

A

H

I

I

D

D

F

F

E

V

P

P

G

G

A

E

L

L

G

G

S

C

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

K

S

F

Y

L

L

E

E

G

D

Q

L

G

G

H

H

T

T

L

F

V

I

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

P

D

N

S

S

V

V

F

F

E

E

R

K

E

E

L

L

V

P

D

D

A

A

E

D

I

I

V

V

I

I

S

S

Q

Q

P

P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

A

A

E

E

R

R

I

I

V

A

K

K

A

A

A

K

R

K

L

L

K

K

L

L

A

A

I

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

N

A

A

I

I

D

K

R

A

G

G

I

I

T

T

V

V

A

A

E

E

V

E

T

T

Y

F

C

S

N
|
N

S

G

I

I

S

C

V
|
V

S

A

E

E

H

H

V

A

V

V

M

M

I

I

L

L

S

A

L

L

A

V

R

R

N

N

Y

Y

I

L

P

P

S

S

Y

H

Q

K

W

I

V

A

V

E

K

E

G

G

W

W

N

N

V

I

A

A

D

D

C

C

V

V

A

S

R

R

S

S

Y

Y

D

D

I

L

E

E

G

G

M

M

D

H

I

V

G

G

T

T

V

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

T

L

A

A

V

V

L

L

R

R

L

L

K

K

P

P

F

F

D

D

V

V

K

K

L

L

H

H

Y

Y

T

T

D
x
A

R
|
R

H

H

R

R

L

S

P

P

D

R

E

A

V

I

A

E

K

D

E

E

L

L

G

G

V

L

T

T

F

Y

H

H

Q

A

T

T

A

A

A

E

E

E

M

M

V

A

P

E

V

V

C

C

D

D

V

V

V

I

T

S

I

I

N

H

A
|
A

P
|
P

L

L

H
x
Y

P

P

E

A

T

T

E

E

N

H

L

L

F

F

N

N

E

A

A

K

M

V

I

L

G

N

K

K

M

M

K

R

R

H

G

G

A

S

Y

Y

L

L

V

V

N

N

T

T

A

A

R
|
R

G

A

K

E

I

I

C

C

N

D

R

R

D

D

A

D

V

I

A

V

R

R

A

A

L

L

E

E

S

S

G

G

Q

Q

L

L

A

A

G

G

Y

Y

A

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q
|
Q

P

P

A

A

P

P

K

A

D

N

H

H

P

P

W

W

R

R

S

N

M

M

P

P

H

H

H

N

G

G

M

M

T

T

P

P

H
|
H

I

M

S

S

G
|
G

S

S

L

L

S

S

A

G

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

W

W

F

F

E

E

G

N

R

R

P

P

I

I

R

R

E

D

E

E

Y

Y

L

L

I

I

V

V

S

S

G

N

G

G

K

K

L

L

A

A

G

G

A

T

G

G

A

A

H

K

S
|
S

Y

Y

S

G

A

V

G

G

active site: N146 (= N149), R284 (= R287), D308 (= D311), Q313 (= Q316), H332 (= H335)
binding nicotinamide-adenine-dinucleotide: F98 (= F101), I122 (= I125), N146 (= N149), V150 (= V153), A198 (≠ G201), G200 (= G203), R201 (= R204), I202 (= I205), A221 (≠ D224), R222 (= R225), A255 (= A258), P256 (= P259), Y258 (≠ H261), R284 (= R287), H332 (= H335), G335 (= G338), S380 (= S383)

4xybA Granulicella m. Formate dehydrogenase (fdh) in complex with NADP(+) and nan3 (see paper)
73% identity, 96% coverage: 4:387/401 of query aligns to 1:384/384 of 4xybA

query
sites
4xybA

A

A

K

K

V

V

A

L

C

C

V

V

L

L

Y

Y

D

D

P

P

V

T

D

S

G

G

Y

Y

P

P

T

P

A

L

Y

Y

A

A

R

R

D

N

G

A

L

I

P

P

T

K

L

I

E

E

R

R

Y

Y

P

P

G

D

G

G

Q

Q

T

T

L

V

P

P

T

N

P

P

K

K

A

H

I

I

D

D

F

F

E

V

P

P

G

G

A

E

L

L

G

G

S

C

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

K

S

F

Y

L

L

E

E

G

D

Q

L

G

G

H

H

T

T

L

F

V

I

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

P

D

N

S

S

V

V

F

F

E

E

R

K

E

E

L

L

V

P

D

D

A

A

E

D

I

I

V

V

I

I

S

S

Q

Q

P
|
P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

A

A

E

E

R

R

I

I

V

A

K

K

A

A

A

K

R

K

L

L

K

K

L

L

A

A

I

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

N

A

A

I

I

D

K

R

A

G

G

I

I

T

T

V

V

A

A

E

E

V

E

T

T

Y

F

C

S

N
|
N

S

G

I

I

S

C

V
|
V

S

A

E

E

H

H

V

A

V

V

M

M

I

I

L

L

S

A

L

L

A

V

R

R

N

N

Y

Y

I

L

P

P

S

S

Y

H

Q

K

W

I

V

A

V

E

K

E

G

G

W

W

N

N

V

I

A

A

D

D

C

C

V

V

A

S

R

R

S

S

Y

Y

D

D

I

L

E

E

G

G

M

M

D

H

I

V

G

G

T

T

V

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

T

L

A

A

V

V

L

L

R

R

L

L

K

K

P

P

F

F

D

D

V

V

K

K

L

L

H

H

Y

Y

T

T

D

A

R
|
R

H
|
H

R

R

L

S

P

P

D

R

E

A

V

I

A

E

K

D

E

E

L

L

G

G

V

L

T

T

F

Y

H

H

Q

A

T

T

A

A

A

E

E

E

M

M

V

A

P

E

V

V

C

C

D

D

V

V

V

I

T

S

I

I

N

H

A

A

P
|
P

L

L

H
x
Y

P

P

E

A

T
|
T

E

E

N

H

L

L

F

F

N

N

E

A

A

K

M

V

I

L

G

N

K

K

M

M

K

R

R

H

G

G

A

S

Y

Y

L

L

V

V

N

N

T
|
T

A
|
A

R
|
R

G

A

K

E

I

I

C

C

N

D

R

R

D

D

A

D

V

I

A

V

R

R

A

A

L

L

E

E

S

S

G

G

Q

Q

L

L

A

A

G

G

Y

Y

A

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q
|
Q

P

P

A

A

P

P

K

A

D

N

H

H

P

P

W

W

R

R

S

N

M

M

P

P

H

H

H

N

G

G

M

M

T

T

P

P

H
|
H

I

M

S
|
S

G
|
G

S

S

L

L

S

S

A

G

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

W

W

F

F

E

E

G

N

R

R

P

P

I

I

R

R

E

D

E

E

Y

Y

L

L

I

I

V

V

S

S

G

N

G

G

K

K

L

L

A

A

G

G

A

T

G

G

A

A

H

K

S
|
S

Y

Y

S

G

A

V

G

G

active site: N146 (= N149), R284 (= R287), D308 (= D311), Q313 (= Q316), H332 (= H335)
binding azide ion: P97 (= P100), F98 (= F101), I122 (= I125), R284 (= R287), H332 (= H335)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I122 (= I125), N146 (= N149), V150 (= V153), A198 (≠ G201), G200 (= G203), R201 (= R204), I202 (= I205), R222 (= R225), H223 (= H226), P256 (= P259), Y258 (≠ H261), T261 (= T264), T282 (= T285), A283 (= A286), R284 (= R287), D308 (= D311), H332 (= H335), S334 (= S337), G335 (= G338), S380 (= S383)

7ya4A Formate dehydrogenase from novosphingobium sp. Ap12 with NAD and azide
73% identity, 95% coverage: 4:385/401 of query aligns to 1:382/384 of 7ya4A

query
sites
7ya4A

A

A

K

K

V

I

A

L

C

C

V

V

L

L

Y

Y

D

P

D

D

P

P

V

V

D

N

G

G

Y

Y

P

P

T

K

A

T

Y

Y

A

A

R

R

D

D

G

E

L

I

P

P

T

N

L

I

E

T

R

V

Y

Y

P

D

G

N

G

G

Q

Q

T

T

L

A

P

P

T

T

P

P

K

K

A

A

I

I

D

D

F

F

E

K

P

P

G

G

A

E

L

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

K

K

F

Y

L

L

E

E

G

G

Q

L

G

G

H

H

T

E

L

F

V

I

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

P

D

D

S

S

V

E

F

F

E

E

R

K

E

H

L

L

V

P

D

D

A

A

E

E

I

I

V

I

I

I

S

S

Q

Q

P
|
P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

G

A

P

E

E

R

R

I

L

V

A

K

K

A

A

A

K

R

K

L

L

K

K

L

L

A

A

I

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

E

A

S

A

A

I

I

D

K

R

H

G

G

I

V

T

T

V

V

A

A

E

E

V

V

T

T

Y

G

C

S

N
|
N

S
|
S

I

I

S

S

V
|
V

S

S

E

E

H

H

V

A

V

V

M

M

I

I

L

L

S

S

L

L

A

V

R

R

N

N

Y

Y

I

I

P

P

S

A

Y

H

Q

E

W

W

V

A

V

E

K

K

G

G

W

W

N

N

V

I

A

A

D

D

C

C

V

V

A

E

R

R

S

S

Y

Y

D

D

I

V

E

E

G

G

M

M

D

H

I

V

G

G

T

T

V

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

T

L

A

A

V

I

L

L

R

K

R

R

L

M

K

K

P

P

F

F

D

D

V

V

K

H

L

L

H

H

Y

Y

T
x
Y

D

A

R
|
R

H

H

R

R

L

L

P

S

D

K

E

E

V

E

A

E

K

E

E

E

L

L

G

G

V

L

T

T

F

F

H

H

Q

E

T

N

A

V

A

E

E

D

M

M

V

V

P

K

V

V

C

C

D

D

V

V

T

T

I

I

N

N

A

A

P
|
P

L

L

H
|
H

P

P

E
|
E

T
|
T

E

H

N

H

L

M

F

F

N

D

E

E

A

A

M

M

I

I

G

K

K

K

M

M

K

K

R

R

G

G

A

A

Y

Y

L

I

V

V

N

N

T
|
T

A
|
A

R
|
R

G

A

K

E

I

I

C

C

N

D

R

R

D

D

A

A

V

I

A

V

R

R

A

A

L

V

E

E

S

S

G

G

Q

H

L

L

A

A

G

G

Y

Y

A

A

G

G

D
|
D

V

V

W

W

F

N

P

P

Q

Q

P

P

A

A

P

P

K

A

D

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

H

W

H

N

G

G

M

M

T

T

P

P

H
|
H

I

M

S
|
S

G
|
G

S

T

S

S

L

L

S

S

A

G

Q

Q

A

A

R

R

Y

Y

A

T

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

W

W

F

F

E

E

G

G

R

R

P

P

I

I

R

R

E

E

E

D

Y

Y

L

V

I

I

V

V

S

D

G

G

K

K

L

L

A

A

G

G

A

T

G

G

A

A

H

L

S
|
S

Y

Y

S

T

binding azide ion: P97 (= P100), F98 (= F101), I122 (= I125), R284 (= R287), H332 (= H335)
binding nicotinamide-adenine-dinucleotide: I122 (= I125), N146 (= N149), S147 (= S150), V150 (= V153), A198 (≠ G201), G200 (= G203), R201 (= R204), I202 (= I205), Y220 (≠ T223), R222 (= R225), P256 (= P259), H258 (= H261), E260 (= E263), T261 (= T264), T282 (= T285), A283 (= A286), R284 (= R287), D308 (= D311), H332 (= H335), S334 (= S337), G335 (= G338), S380 (= S383)

7ya3B Formate dehydrogenase from novosphingobium sp. Ap12 with NADP and azide
73% identity, 95% coverage: 4:385/401 of query aligns to 1:382/384 of 7ya3B

query
sites
7ya3B

A

A

K

K

V

I

A

L

C

C

V

V

L

L

Y

Y

D

P

D

D

P

P

V

V

D

N

G

G

Y

Y

P

P

T

K

A

T

Y

Y

A

A

R

R

D

D

G

E

L

I

P

P

T

N

L

I

E

T

R

V

Y

Y

P

D

G

N

G

G

Q

Q

T

T

L

A

P

P

T

T

P

P

K

K

A

A

I

I

D

D

F

F

E

K

P

P

G

G

A

E

L

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

K

K

F

Y

L

L

E

E

G

G

Q

L

G

G

H

H

T

E

L

F

V

I

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

P

D

D

S

S

V

E

F

F

E

E

R

K

E

H

L

L

V

P

D

D

A

A

E

E

I

I

V

I

I

I

S

S

Q

Q

P

P

F

F

W

W

P

P

A

A

Y

Y

L

L

T

G

A

P

E

E

R

R

I

L

V

A

K

K

A

A

A

K

R

K

L

L

K

K

L

L

A

A

I

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

E

A

S

A

A

I

I

D

K

R

H

G

G

I

V

T

T

V

V

A

A

E

E

V

V

T

T

Y

G

C

S

N
|
N

S

S

I

I

S

S

V
|
V

S

S

E

E

H

H

V

A

V

V

M

M

I

I

L

L

S

S

L

L

A

V

R

R

N

N

Y

Y

I

I

P

P

S

A

Y

H

Q

E

W

W

V

A

V

E

K

K

G

G

W

W

N

N

V

I

A

A

D

D

C

C

V

V

A

E

R

R

S

S

Y

Y

D

D

I

V

E

E

G

G

M

M

D

H

I

V

G

G

T

T

V

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

T

L

A

A

V

I

L

L

R

K

R

R

L

M

K

K

P

P

F

F

D

D

V

V

K

H

L

L

H

H

Y

Y

T

Y

D

A

R
|
R

H
|
H

R

R

L

L

P

S

D

K

E

E

V

E

A

E

K

E

E

E

L

L

G

G

V

L

T

T

F

F

H

H

Q

E

T

N

A

V

A

E

E

D

M

M

V

V

P

K

V

V

C

C

D

D

V

V

T

T

I

I

N

N

A

A

P
|
P

L

L

H
|
H

P

P

E
|
E

T

T

E

H

N

H

L

M

F

F

N

D

E

E

A

A

M

M

I

I

G

K

K

K

M

M

K

K

R

R

G

G

A

A

Y

Y

L

I

V

V

N

N

T
|
T

A
|
A

R
|
R

G

A

K

E

I

I

C

C

N

D

R

R

D

D

A

A

V

I

A

V

R

R

A

A

L

V

E

E

S

S

G

G

Q

H

L

L

A

A

G

G

Y

Y

A

A

G

G

D
|
D

V

V

W

W

F

N

P

P

Q

Q

P

P

A

A

P

P

K

A

D

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

H

W

H

N

G

G

M

M

T

T

P

P

H
|
H

I

M

S
|
S

G
|
G

S

T

S

S

L

L

S

S

A

G

Q

Q

A

A

R

R

Y

Y

A

T

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

W

W

F

F

E

E

G

G

R

R

P

P

I

I

R

R

E

E

E

D

Y

Y

L

V

I

I

V

V

S

D

G

G

K

K

L

L

A

A

G

G

A

T

G

G

A

A

H

L

S
|
S

Y

Y

S

T

binding azide ion: I122 (= I125), R284 (= R287), H332 (= H335)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I122 (= I125), N146 (= N149), V150 (= V153), A198 (≠ G201), G200 (= G203), R201 (= R204), I202 (= I205), R222 (= R225), H223 (= H226), P256 (= P259), H258 (= H261), E260 (= E263), T282 (= T285), A283 (= A286), R284 (= R287), D308 (= D311), H332 (= H335), S334 (= S337), G335 (= G338), S380 (= S383)

Q9S7E4 Formate dehydrogenase, chloroplastic/mitochondrial; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Arabidopsis thaliana (Mouse-ear cress)
56% identity, 81% coverage: 52:377/401 of query aligns to 55:380/384 of Q9S7E4

query
sites
Q9S7E4

L

F

L

L

G

G

S

C

V

V

S

E

G

N

E

A

L

L

G

G

L

I

R

R

K

D

F

W

L

L

E

E

G

S

Q

Q

G

G

H

H

T

Q

L

Y

V

I

V

V

T

T

S

D

D

D

K

K

D

E

G

G

P

P

D

D

S

C

V

E

F

L

E

E

R

K

E

H

L

I

V

P

D

D

A

L

E

H

I

V

V

L

I

I

S

S

Q

T

P

P

F

F

W

H

P

P

A

A

Y

Y

L

V

T

T

A

A

E

E

R

R

I

I

V

K

K

K

A

A

A

K

R

N

L

L

K

K

L

L

A

L

I

L

T

T

A

A

G

G

I

I

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

Q

A

A

I

A

D

A

R

A

G

G

I

L

T

T

V

V

A

A

E

E

V

V

T

T

Y

G

C

S

N

N

S

V

I

V

S

S

V

V

S

A

E

E

H

D

V

E

V

L

M

M

M

R

I

I

L

L

S

I

L

L

A

M

R

R

N

N

Y

F

I

V

P

P

S

G

Y

Y

Q

N

W

Q

V

V

K

K

G

G

G

E

W

W

N

N

V

V

A

A

D

G

C

I

V

A

A

Y

R

R

S

A

Y

Y

D

D

I

L

E

E

G

G

M

K

D

T

I

I

G

G

T

T

V

V

G

G

A

A

G

G

R
|
R

I
|
I

G

G

T

K

A

L

V

L

L

L

R

Q

R

R

L

L

K

K

P

P

F

F

D

G

V

C

K

N

L

L

H

L

Y

Y

T

H

D
|
D

R

R

H

L

R

Q

L

M

P

A

D

P

E

E

V

L

A

E

K

K

E

E

L

T

G

G

V

A

T

K

F

F

H

V

Q

E

T

D

A

L

A

N

E

E

M

M

V

L

P

P

V

K

C

C

D

D

V

V

V

I

T

V

I

I

N

N

A

M

P
|
P

L
|
L

H
x
T

P
x
E

E
x
K

T

T

E

R

N

G

L

M

F

F

N

N

E

K

A

E

M

L

I

I

G

G

K

K

M

L

K

K

R

K

G

G

A

V

Y

L

L

I

V

V

N

N

T
x
N

A

A

R

R

G

G

K

A

I

I

C

M

N

E

R

R

D

Q

A

A

V

V

A

V

R

D

A

A

L

V

E

E

S

S

G

G

Q

H

L

I

A

G

G

G

Y

Y

A

S

G

G

D
|
D

V

V

W

W

F

D

P

P

Q

Q

P

P

A

A

P

P

K

K

D

D

H

H

P

P

W

W

R

R

S

Y

M

M

P

P

H

N

H

Q

G

A

M

M

T

T

P

P

H
|
H

I
x
T

S
|
S

G
|
G

S

T

L

I

S

D

A

A

Q

Q

A

L

R

R

Y

Y

A

A

G

G

T

T

R

K

E

D

I

M

L

L

E

E

C

R

W

Y

F

F

E

K

G

G

R

E

P

D

I

F

R

P

E

T

E

E

Y

N

L

Y

I

I

V

V

S

K

G

D

G

G

K

E

L

L

A

A

RI 207:208 (= RI 204:205) binding
D227 (= D224) binding
PLTEK 262:266 (≠ PLHPE 259:263) binding
N288 (≠ T285) binding
D314 (= D311) binding
HTSG 338:341 (≠ HISG 335:338) binding

Sites not aligning to the query:

1:29 modified: transit peptide, Chloroplast and mitochondrion

3n7uA NAD-dependent formate dehydrogenase from higher-plant arabidopsis thaliana in complex with NAD and azide
56% identity, 81% coverage: 52:377/401 of query aligns to 22:347/351 of 3n7uA

query
sites
3n7uA

L

F

L

L

G

G

S

C

V

V

S

E

G

N

E

A

L

L

G

G

L

I

R

R

K

D

F

W

L

L

E

E

G

S

Q

Q

G

G

H

H

T

Q

L

Y

V

I

V

V

T

T

S

D

D

D

K

K

D

E

G

G

P

P

D

D

S

C

V

E

F

L

E

E

R

K

E

H

L

I

V

P

D

D

A

L

E

H

I

V

V

L

I

I

S

S

Q

T

P
|
P

F
|
F

W

H

P

P

A

A

Y

Y

L

V

T

T

A

A

E

E

R

R

I

I

V

K

K

K

A

A

A

K

R

N

L

L

K

K

L

L

A

L

I

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

Q

A

A

I

A

D

A

R

A

G

G

I

L

T

T

V

V

A

A

E

E

V

V

T

T

Y

G

C

S

N
|
N

S

V

I

V

S

S

V
|
V

S

A

E

E

H

D

V

E

V

L

M

M

M

R

I

I

L

L

S

I

L

L

A

M

R

R

N

N

Y

F

I

V

P

P

S

G

Y

Y

Q

N

W

Q

V

V

K

K

G

G

G

E

W

W

N

N

V

V

A

A

D

G

C

I

V

A

A

Y

R

R

S

A

Y

Y

D

D

I

L

E

E

G

G

M

K

D

T

I

I

G

G

T

T

V

V

G

G

A

A

G
|
G

R
|
R

I
|
I

G

G

T

K

A

L

V

L

L

L

R

Q

R

R

L

L

K

K

P

P

F

F

D

G

V

C

K

N

L

L

H

L

Y

Y

T

H

D
|
D

R
|
R

H

L

R

Q

L

M

P

A

D

P

E

E

V

L

A

E

K

K

E

E

L

T

G

G

V

A

T

K

F

F

H

V

Q

E

T

D

A

L

A

N

E

E

M

M

V

L

P

P

V

K

C

C

D

D

V

V

V

I

T

V

I

I

N

N

A
x
M

P
|
P

L

L

H

T

P

E

E

K

T

T

E

R

N

G

L

M

F

F

N

N

E

K

A

E

M

L

I

I

G

G

K

K

M

L

K

K

R

K

G

G

A

V

Y

L

L

I

V

V

N

N

T
x
N

A
|
A

R
|
R

G

G

K

A

I

I

C

M

N

E

R

R

D

Q

A

A

V

V

A

V

R

D

A

A

L

V

E

E

S

S

G

G

Q

H

L

I

A

G

G

G

Y

Y

A

S

G

G

D
|
D

V

V

W

W

F

D

P

P

Q
|
Q

P

P

A

A

P

P

K

K

D

D

H

H

P

P

W

W

R

R

S

Y

M

M

P

P

H

N

H

Q

G

A

M

M

T

T

P

P

H
|
H

I

T

S
|
S

G
|
G

S

T

L

I

S

D

A

A

Q

Q

A

L

R

R

Y

Y

A

A

G

G

T

T

R

K

E

D

I

M

L

L

E

E

C

R

W

Y

F

F

E

K

G

G

R

E

P

D

I

F

R

P

E

T

E

E

Y

N

L

Y

I

I

V

V

S

K

G

D

G

G

K

E

L

L

A

A

active site: N119 (= N149), R257 (= R287), D281 (= D311), Q286 (= Q316), H305 (= H335)
binding azide ion: P70 (= P100), F71 (= F101), I95 (= I125), R257 (= R287), H305 (= H335)
binding nicotinamide-adenine-dinucleotide: I95 (= I125), N119 (= N149), V123 (= V153), G173 (= G203), R174 (= R204), I175 (= I205), D194 (= D224), R195 (= R225), M228 (≠ A258), P229 (= P259), N255 (≠ T285), A256 (= A286), R257 (= R287), D281 (= D311), H305 (= H335), S307 (= S337), G308 (= G338)

3jtmA Structure of recombinant formate dehydrogenase from arabidopsis thaliana
56% identity, 81% coverage: 52:377/401 of query aligns to 22:347/351 of 3jtmA

query
sites
3jtmA

L

F

L

L

G

G

S

C

V

V

S

E

G

N

E

A

L

L

G

G

L

I

R

R

K

D

F

W

L

L

E

E

G

S

Q

Q

G

G

H

H

T

Q

L

Y

V

I

V

V

T

T

S

D

D

D

K

K

D

E

G

G

P

P

D

D

S

C

V

E

F

L

E

E

R

K

E

H

L

I

V

P

D

D

A

L

E

H

I

V

V

L

I

I

S

S

Q

T

P

P

F

F

W

H

P

P

A

A

Y

Y

L

V

T

T

A

A

E

E

R

R

I

I

V

K

K

K

A

A

A

K

R

N

L

L

K

K

L

L

A

L

I

L

T

T

A

A

G

G

I

I

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

Q

A

A

I

A

D

A

R

A

G

G

I

L

T

T

V

V

A

A

E

E

V

V

T

T

Y

G

C

S

N
|
N

S

V

I

V

S

S

V

V

S

A

E

E

H

D

V

E

V

L

M

M

M

R

I

I

L

L

S

I

L
|
L

A

M

R

R

N

N

Y

F

I

V

P

P

S

G

Y

Y

Q

N

W

Q

V

V

K

K

G

G

G

E

W

W

N

N

V

V

A

A

D

G

C

I

V

A

A

Y

R

R

S

A

Y

Y

D

D

I

L

E

E

G

G

M

K

D

T

I

I

G

G

T

T

V

V

G

G

A

A

G

G

R

R

I

I

G

G

T

K

A

L

V

L

L

L

R

Q

R

R

L

L

K

K

P

P

F

F

D

G

V

C

K

N

L

L

H

L

Y

Y

T

H

D

D

R

R

H

L

R

Q

L

M

P

A

D

P

E

E

V

L

A

E

K

K

E

E

L

T

G

G

V

A

T

K

F

F

H

V

Q

E

T

D

A

L

A

N

E

E

M

M

V

L

P

P

V

K

C

C

D

D

V

V

V

I

T

V

I

I

N

N

A

M

P

P

L

L

H

T

P

E

E

K

T

T

E

R

N

G

L

M

F

F

N

N

E

K

A

E

M

L

I

I

G

G

K

K

M

L

K

K

R

K

G

G

A

V

Y

L

L

I

V

V

N

N

T

N

A

A

R
|
R

G

G

K

A

I

I

C

M

N

E

R

R

D

Q

A

A

V

V

A

V

R

D

A

A

L

V

E

E

S

S

G

G

Q

H

L

I

A
x
G

G

G

Y

Y

A

S

G

G

D
|
D

V

V

W

W

F

D

P

P

Q
|
Q

P

P

A

A

P

P

K

K

D

D

H

H

P

P

W

W

R

R

S

Y

M

M

P

P

H

N

H

Q

G

A

M

M

T

T

P

P

H
|
H

I

T

S

S

G

G

S

T

L

I

S

D

A

A

Q

Q

A

L

R

R

Y

Y

A

A

G

G

T

T

R

K

E

D

I

M

L

L

E

E

C

R

W

Y

F

F

E

K

G

G

R

E

P

D

I

F

R

P

E

T

E

E

Y

N

L

Y

I

I

V

V

S

K

G

D

G

G

K

E

L

L

A

A

active site: N119 (= N149), R257 (= R287), D281 (= D311), Q286 (= Q316), H305 (= H335)
binding azide ion: L134 (= L164), G276 (≠ A306)

7arzA Ternary complex of NAD-dependent formate dehydrogenase from physcomitrium patens
53% identity, 81% coverage: 52:377/401 of query aligns to 31:357/361 of 7arzA

query
sites
7arzA

L

F

L

L

G

G

S

C

V

V

S

E

G

N

E

A

L

L

G

G

L

I

R

R

K

E

F

W

L

L

E

E

G

S

Q

K

G

G

H

H

T

K

L

Y

V

V

T

T

S

S

D

D

K

K

D

D

G

G

P

P

D

D

S

S

V

E

F

L

E

D

R

K

E

E

L

L

V

A

D

D

A

A

E

H

I

I

V

L

I

I

S
x
T

Q

T

P
|
P

F
|
F

W

H

P

P

A

A

Y

Y

L

M

T

T

A

K

E

E

R

R

I

L

V

A

K

K

A

A

A

K

R

N

L

L

K

E

L

L

A
x
L

I
x
V

T
|
T

A

A

G
|
G

I
x
V

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

H

A

A

I

A

D

E

R

K

G

G

I

L

T

T

V

V

A

S

E

E

V

V

T

T

Y

G

C

S

N
|
N

S

V

I

T

S

S

V
|
V

S

A

E

E

H

D

V

E

V

V

M

L

M

R

I

I

L

L

S

V

L

L

A

V

R

R

N

N

Y

F

I

A

P

P

S

G

Y

W

Q

K

W

Q

V

V

V

S

K

E

G

G

W

W

N

N

V

V

A

A

D

A

C

V

V

V

A

H

R

H

S

A

Y

Y

D

D

I

L

E

I

G

D

M

R

D

T

I

V

G

G

T

T

V

V

G

G

A

G

G
|
G

R
|
R

I
|
I

G

G

T

Q

A

E

V

L

L

M

R

K

R

R

L

L

K

K

P

G

F

F

D

G

V

L

K

K

-

E

L

M

H

L

Y

Y

T

Y

D
|
D

R
|
R

H

N

R

S

L

L

P

G

D

A

E

E

V

R

A

E

K

K

E

E

L

L

G

G

V

C

T

K

F

R

H

E

Q

T

T

D

A

L

A

D

E

T

M

M

V

L

P

S

V

K

C

C

D

D

V

V

T

V

I

V

N

N

A
x
T

P
|
P

L

L

H

T

P

D

E
x
Q

T

T

E

R

N

G

L

L

F

F

N

N

E

K

A

E

M

R

I

I

G

A

K

K

M

M

K

K

R

K

G

G

A

A

Y

Y

L

L

V

V

N

N

T
x
N

A
|
A

R
|
R

G

G

K

A

I

I

C

A

N

D

R

T

D

E

A

A

V

V

A

K

R

E

A

A

L

C

E

E

S

S

G

G

Q

H

L

L

A

G

G

G

Y

Y

A

G

G

G

D
|
D

V

V

W

W

F

N

P

A

Q

Q

P

P

A

A

P

G

K

K

D

D

H

H

P

P

W

W

R

R

S

Y

M

M

P

P

H

N

H

H

G

A

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

S

T

L

L

S

D

A

A

Q

Q

A

K

R

R

Y

F

A

A

G

G

T

T

R

K

E

D

I

M

L

I

E

D

C

R

W

W

F

L

E

K

G

H

R

E

P

A

I

F

R

P

E

E

E

Q

Y

N

L

Y

I

I

V

V

S

R

G

E

G

G

K

K

L

L

A

A

binding azide ion: T77 (≠ S98), P79 (= P100), F80 (= F101), L99 (≠ A120), V100 (≠ I121), T101 (= T122), G103 (= G124), V104 (≠ I125), R267 (= R287), H315 (= H335)
binding nicotinamide-adenine-dinucleotide: V104 (≠ I125), N128 (= N149), V132 (= V153), G182 (= G203), R183 (= R204), I184 (= I205), D204 (= D224), R205 (= R225), T238 (≠ A258), P239 (= P259), Q243 (≠ E263), N265 (≠ T285), A266 (= A286), R267 (= R287), D291 (= D311), H315 (= H335), S317 (= S337), G318 (= G338)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 359

6t8zAAA Formate dehydrogenase (see paper)
57% identity, 82% coverage: 49:377/401 of query aligns to 24:360/373 of 6t8zAAA

query
sites
6t8zAAA

P

P

G

G

A

-

L

L

G

G

S

T

V

T

S

E

G

N

E

E

L

L

G

G

L

L

R

R

K

K

F

W

L

L

E

E

G

D

Q

Q

G

G

H

H

T

T

L

L

V

V

V

T

T

T

S

S

D

D

K

K

D

D

G

R

P

E

D

G

S

S

V

T

F

F

E

D

R

R

E

E

L

L

V

E

D

D

A

A

E

E

I

I

V

I

I

I

S

T

Q

T

P

P

F

F

W

H

P

P

A

G

Y

Y

L

L

T

T

A

A

E

E

R

R

I

L

V

A

K

R

A

A

A

K

R

K

L

L

K

K

L

L

A

A

I

V

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

D

A

A

-

N

-

K

I

T

D

N

R

G

G

G

I

I

T

T

V

V

A

A

E

E

V

V

T

T

Y

G

C

S

N
|
N

S

V

I

V

S

S

V
|
V

S

A

E

E

H

H

V

V

M

M

M

T

I

I

L

L

S

V

L

L

A

V

R

R

N

N

Y

F

I

V

P

P

S

A

Y

H

Q

E

W

Q

V

I

V

E

K

A

G

G

G

R

W

W

N

D

V

V

A

A

D

E

C

V

V

A

A

K

R

D

S

E

Y

Y

D

D

I

L

E

E

G

G

M

K

D

V

I

V

G

G

T

T

V

V

G
|
G

A

V

G
|
G

R
|
R

I
|
I

G

G

T

E

A

R

V

V

L

L

R

R

L

L

K

K

P

G

F

F

D

D

V

C

K

K

-

E

L

L

H

L

Y

Y

T
x
Y

D
|
D

R
x
Y

H
x
Q

R

P

L

L

P

S

D

P

E

E

V

K

A

E

K

K

E

E

L

I

G

G

V

C

T

R

F

R

H

V

Q

E

T

N

A

L

A

E

E

E

M

M

V

L

P

A

V

Q

C

C

D

D

V

V

T

T

I

I

N

N

A
x
C

P
|
P

L

L

H
|
H

P

E

E

S

T

T

E

R

N

G

L

L

F

F

N

N

E

K

A

D

M

L

I

I

G

S

K

K

M

M

K

K

R

R

G

G

A

S

Y

W

L

L

V

V

N

N

T
|
T

A
|
A

R
|
R

G

G

K

A

I

I

C

V

N

V

R

K

D

E

A

D

V

V

A

A

R

E

A

A

L

L

E

R

S

T

G

G

Q

H

L

L

A

R

G

G

Y

Y

A

G

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

K

A

D

D

H

H

P

V

W

L

R

R

S

T

M

A

P

K

H

N

-

P

-

F

-

G

H

N

G

A

M

M

T

V

P

P

H
|
H

I

M

S
|
S

G
|
G

S

T

S

S

L

L

S

D

A

A

Q

Q

A

K

R

R

Y

Y

A

A

A

E

G

G

T

V

R

K

E

R

I

I

L

L

E

D

C

S

W

Y

F

L

E

S

G

G

R

R

-

F

P

D

I

Y

R

R

E

P

E

E

Y

D

L

L

I

I

V

V

S

H

G

Q

G

G

K

K

L

Y

A

A

binding nicotinamide-adenine-dinucleotide: I99 (= I125), N125 (= N149), V129 (= V153), G177 (= G201), G179 (= G203), R180 (= R204), I181 (= I205), Y200 (≠ T223), D201 (= D224), Y202 (≠ R225), Q203 (≠ H226), C235 (≠ A258), P236 (= P259), H238 (= H261), T262 (= T285), A263 (= A286), R264 (= R287), D288 (= D311), H317 (= H335), S319 (= S337), G320 (= G338)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 363

6t8yBBB Formate dehydrogenase (see paper)
57% identity, 82% coverage: 49:377/401 of query aligns to 25:361/375 of 6t8yBBB

query
sites
6t8yBBB

P

P

G

G

A

-

L

L

G

G

S

T

V

T

S

E

G

N

E

E

L

L

G

G

L

L

R

R

K

K

F

W

L

L

E

E

G

D

Q

Q

G

G

H

H

T

T

L

L

V

V

V

T

T

T

S

S

D

D

K

K

D

D

G

R

P

E

D

G

S

S

V

T

F

F

E

D

R

R

E

E

L

L

V

E

D

D

A

A

E

E

I

I

V

I

I

I

S

T

Q

T

P

P

F

F

W

H

P

P

A

G

Y

Y

L

L

T

T

A

A

E

E

R

R

I

L

V

A

K

R

A

A

A

K

R

K

L

L

K

K

L

L

A

A

I

V

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

D

A

A

-

N

-

K

I

T

D

N

R

G

G

G

I

I

T

T

V

V

A

A

E

E

V

V

T

T

Y

G

C

S

N
|
N

S

V

I

V

S

S

V
|
V

S

A

E

E

H

H

V

V

M

M

M

T

I

I

L

L

S

V

L

L

A

V

R

R

N

N

Y

F

I

V

P

P

S

A

Y

H

Q

E

W

Q

V

I

V

E

K

A

G

G

G

R

W

W

N

D

V

V

A

A

D

E

C

V

V

A

A

K

R

D

S

E

Y

Y

D

D

I

L

E

E

G

G

M

K

D

V

I

V

G

G

T

T

V

V

G
|
G

A

V

G
|
G

R
|
R

I
|
I

G

G

T

E

A

R

V

V

L

L

R

R

L

L

K

K

P

G

F

F

D

D

V

C

K

K

-

E

L

L

H

L

Y

Y

T
x
Y

D
|
D

R
x
Y

H
x
Q

R

P

L

L

P

S

D

P

E

E

V

K

A

E

K

K

E

E

L

I

G

G

V

C

T

R

F

R

H

V

Q

E

T

N

A

L

A

E

E

E

M

M

V

L

P

A

V

Q

C

C

D

D

V

V

T

T

I

I

N

N

A
x
C

P
|
P

L

L

H
|
H

P

E

E

S

T

T

E

R

N

G

L

L

F

F

N

N

E

K

A

D

M

L

I

I

G

S

K

K

M

M

K

K

R

R

G

G

A

S

Y

W

L

L

V

V

N

N

T
|
T

A
|
A

R
|
R

G

G

K

A

I

I

C

V

N

V

R

K

D

E

A

D

V

V

A

A

R

E

A

A

L

L

E

R

S

T

G

G

Q

H

L

L

A

R

G

G

Y

Y

A

G

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

K

A

D

D

H

H

P

V

W

L

R

R

S

T

M

A

P

K

H

N

-

P

-

F

-

G

H

N

G

A

M

M

T

V

P

P

H
|
H

I

M

S
|
S

G
|
G

S

T

S

S

L

L

S

D

A

A

Q

Q

A

K

R

R

Y

Y

A

A

A

E

G

G

T

V

R

K

E

R

I

I

L

L

E

D

C

S

W

Y

F

L

E

S

G

G

R

R

-

F

P

D

I

Y

R

R

E

P

E

E

Y

D

L

L

I

I

V

V

S

H

G

Q

G

G

K

K

L

Y

A

A

binding 1,4-dihydronicotinamide adenine dinucleotide: I100 (= I125), N126 (= N149), V130 (= V153), G178 (= G201), G180 (= G203), R181 (= R204), I182 (= I205), Y201 (≠ T223), D202 (= D224), Y203 (≠ R225), Q204 (≠ H226), C236 (≠ A258), P237 (= P259), H239 (= H261), T263 (= T285), A264 (= A286), R265 (= R287), D289 (= D311), H318 (= H335), S320 (= S337), G321 (= G338)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 364

6d4bA Crystal structure of candida boidinii formate dehydrogenase v123a mutant complexed with NAD+ and azide
51% identity, 78% coverage: 52:362/401 of query aligns to 20:338/361 of 6d4bA

query
sites
6d4bA

L

L

L

Y

G

G

S

C

V

T

S

E

G

N

E

K

L

L

G

G

L

I

R

A

K

N

F

W

L

L

E

K

G

D

Q

Q

G

G

H

H

T

E

L

L

V

I

V

T

T

T

S

S

D

D

K

K

D

E

G

G

P

G

D

N

S

S

V

V

F

L

E

D

R

Q

E

H

L

I

V

P

D

D

A

A

E

D

I

I

V

I

I

I

S

T

Q

T

P
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P

F
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F

W

H

P

P

A

A

Y

Y

L

I

T

T

A

K

E

E

R

R

I

I

V

D

K

K

A

A

A

K

R

K

L

L

K

K

L

L

A

V

I

V

T

V

A

A

G

G

I
x
V

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

D

A

Y

A

I

I

N

D

Q

R

T

G

G

-

K

I

I

T

S

V

V

A

L

E

E

V

V

T

T

Y

G

C

S

N
|
N

S

V

I

V

S

S

V

A

S

A

E

E

H

H

V

V

V

L

M

M

M

T

I

M

L

L

S

V

L

L

A

V

R

R

N

N

Y

F

I

V

P

P

S

A

Y

H

Q

E

W

Q

V

I

K

N

G

H

G

D

W

W

N

E

V

V

A

A

D

A

C

I

V

A

A

K

R

D

S

A

Y

Y

D

D

I

I

E

E

G

G

M

K

D

T

I

I

G

A

T

T

V

I

G

G

A

A

G
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G

R
|
R

I
|
I

G

G

T

Y

A

R

V

V

L

L

R

E

R

R

L

L

K

V

P

P

F

F

D

N

V

P

K

K

-

E

L

L

H

L

Y

Y

T
x
Y

D
|
D

R
x
Y

H

Q

R

A

L

L

P

P

D

K

E

D

V

A

A

E

K

E

E

K

L

V

G

G

V

A

T

R

F

R

H

V

Q

E

T

N

A

I

A

E

E

E

M

L

V

V

P

A

V

Q

C

A

D

D

V

I

V

V

T

T

I

I

N

N

A
|
A

P
|
P

L

L

H
|
H

P

A

E

G

T
|
T

E

K

N

G

L

L

F

I

N

N

E

K

A

E

M

L

I

L

G

S

K

K

M

F

K

K

R

K

G

G

A

A

Y

W

L

L

V

V

N

N

T
|
T

A
|
A

R
|
R

G

G

K

A

I

I

C

C

N

V

R

A

D

E

A

D

V

V

A

A

R

A

A

A

L

L

E

E

S

S

G

G

Q

Q

L

L

A

R

G

G

Y

Y

A

G

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

K

K

D

D

H

H

P

P

W

W

R

R

S

D

M

M

P

R

H

N

-

K

-

Y

-

G

-

A

-

G

H

N

G

A

M

M

T

T

P

P

H
|
H

I

Y

S
|
S

G
|
G

S

T

L

L

S

D

A

A

Q

Q

A

T

R

R

Y

Y

A

A

A

E

G

G

T

T

R

K

E

N

I

I

L

L

E

E

C

S

W

F

F

F

E

T

G

G

R

K

binding azide ion: P68 (= P100), F69 (= F101), V93 (≠ I125), R258 (= R287), H311 (= H335)
binding nicotinamide-adenine-dinucleotide: V93 (≠ I125), N119 (= N149), G173 (= G203), R174 (= R204), I175 (= I205), Y194 (≠ T223), D195 (= D224), Y196 (≠ R225), A229 (= A258), P230 (= P259), H232 (= H261), T235 (= T264), T256 (= T285), A257 (= A286), R258 (= R287), D282 (= D311), H311 (= H335), S313 (= S337), G314 (= G338)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 357

O13437 Formate dehydrogenase; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Candida boidinii (Yeast) (see 4 papers)
51% identity, 78% coverage: 52:362/401 of query aligns to 20:338/364 of O13437

query
sites
O13437

L

L

L

Y

G

G

S
x
C

V

T

S

E

G

N

E

K

L

L

G

G

L

I

R

A

K

N

F

W

L

L

E

K

G

D

Q

Q

G

G

H

H

T

E

L

L

V

I

V

T

T

T

S

S

D

D

K
|
K

D

E

G

G

P

E

D

T

S

S

V

E

F

L

E

D

R

K

E

H

L

I

V

P

D

D

A

A

E

D

I

I

V

I

I

I

S

T

Q

T

P

P

F
|
F

W

H

P

P

A

A

Y

Y

L

I

T

T

A

K

E

E

R

R

I

L

V

D

K

K

A

A

A

K

R

N

L

L

K

K

L

L

A

V

I

V

T

V

A

A

G

G

I

V

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

D

A

Y

A

I

I

N

D

Q

R

T

G

G

-

K

I

I

T

S

V

V

A

L

E

E

V

V

T

T

Y

G

C

S

N
|
N

S

V

I

V

S

S

V

V

S

A

E

E

H

H

V

V

M

M

M

T

I

M

L

L

S

V

L

L

A

V

R

R

N

N

Y

F

I

V

P

P

S

A

Y

H

Q

E

W

Q

V

I

K

N

G

H

G

D

W

W

N

E

V

V

A

A

D

A

C

I

V

A

A

K

R

D

S

A

Y

Y

D

D

I

I

E

E

G

G

M

K

D

T

I

I

G

A

T

T

V

I

G

G

A

A

G

G

R

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I
|
I

G

G

T

Y

A

R

V

V

L

L

R

E

R

R

L

L

K

L

P

P

F

F

D

N

V

P

K

K

-

E

L

L

H

L

Y

Y

T

Y

D

D

R

Y

H
x
Q

R

A

L

L

P

P

D

K

E

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V

A

A

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K

E

E

K

L

V

G

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V

A

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N

A

I

A

E

E

E

M

L

V

V

P

A

V

Q

C

A

D

D

V

I

V

V

T

T

I

V

N

N

A

A

P

P

L

L

H

H

P

A

E

G

T

T

E

K

N

G

L

L

F

I

N

N

E

K

A

E

M

L

I

L

G

S

K

K

M

F

K

K

R

K

G

G

A

A

Y

W

L

L

V

V

N

N

T

T

A

A

R
|
R

G

G

K

A

I

I

C
|
C

N

V

R

A

D

E

A

D

V

V

A

A

R

A

A

A

L

L

E

E

S

S

G

G

Q

Q

L

L

A

R

G

G

Y

Y

A

G

G

G

D

D

V

V

W

W

F

F

P

P

Q
|
Q

P
|
P

A

A

P

P

K

K

D

D

H

H

P

P

W

W

R

R

S

D

M

M

P

R

H

N

-

K

-

Y

-

G

-

A

-

G

H

N

G

A

M

M

T

T

P

P

H
|
H

I

Y

S

S

G

G

S

T

L

L

S

D

A

A

Q

Q

A

T

R

R

Y

Y

A

A

A

E

G

G

T

T

R
x
K

E

N

I

I

L

L

E

E

C

S

W

F

F

F

E

T

G

G

R

K

C23 (≠ S55) mutation to S: Slight increase in substrate affinity for formate but no change in affinity for NAD, 9 degrees Celsius decrease in thermal stability compared to the wild-type, significantly higher stability compared to wild-type under biotransformation conditions, significantly more stable in the presence of CuCl(2); when associated with A-262. Large increase in substrate affinity for formate but no significant change in affinity for NAD, 13 degrees Celsius decrease in thermal stability compared to the wild-type, significantly more stable in the presence of CuCl(2); when associated with V-262. No significant change in affinity for formate or NAD, 5 degrees Celsius decrease in thermal stability compared to the wild-type, significantly higher stability compared to wild-type under biotransformation conditions, and significantly more stable in the presence of CuCl(2).
K47 (= K79) mutation to E: Slight increase in substrate affinity for formate and also affinity for NAD increases by half after 2 weeks. Also after 4 months affinity for formate increases by more than half and affinity for NAD increases by more than half. Retains 84% of residual activity after incubation for 20 minutes at a thermal inactivation temperature of 55 degrees Celsius in samples stored for 2 weeks compared to wild-type which loses 50% of its activity at 55 degrees Celsius.
F69 (= F101) mutation to A: 2-fold decrease in substrate affinity for formate, but no significant change in affinity for NAD. A significant reduction in catalytic activity compared to the wild-type.
N119 (= N149) mutation to A: 94-fold decrease in substrate affinity for formate and 2700-fold decrease in substrate affinity for NAD. A significant reduction in catalytic activity compared to the wild-type; when associated with A-311.; mutation to H: 80-fold decrease in substrate affinity for formate and a 1250-fold decrease in substrate affinity for NAD. A significant reduction in catalytic activity compared to the wild-type.
I175 (= I205) mutation to A: 2-fold decrease in substrate affinity for formate and a 12-fold decrease in substrate affinity for NAD. A significant reduction in catalytic activity compared to the wild-type.
Q197 (≠ H226) mutation to L: 4-fold decrease in substrate affinity for formate but no significant change in affinity for NAD compared to the wild-type.
R258 (= R287) mutation to A: No catalytic activity.
C262 (= C291) mutation to A: Slight increase in substrate affinity for formate but no change in affinity for NAD, 9 degrees Celsius decrease in thermal stability compared to the wild-type, greater stability at a higher pH compared to the wild-type; when associated with S-23.; mutation to V: Large increase in substrate affinity for formate but no significant change in affinity for NAD, 13 degrees Celsius decrease in thermal stability compared to the wild-type; when associated with S-23. Great increase in substrate affinity for formate and NAD and 8 degrees Celsius decrease in thermal stability compared to the wild-type.
Q287 (= Q316) mutation to A: 2-fold decrease in substrate affinity for formate and 3-fold decrease in substrate affinity for NAD compared to the wild-type; when associated with A-311.; mutation to E: 380-fold decrease in substrate affinity for formate and 3-fold decrease in substrate affinity for NAD compared to the wild-type; when associated with T-288. No significant decrease in substrate affinity for formate but a 4-fold decrease in substrate affinity for NAD and a significant reduction in catalytic activity compared to the wild-type, a more acidic pH is seen than in the wild-type, preventing formate binding by a single ionization of a group compared to that of the wild-type.
P288 (= P317) mutation to T: 380-fold decrease in substrate affinity for formate and 3-fold decrease in substrate affinity for NAD compared to the wild-type; when associated with E-287.
H311 (= H335) mutation to A: 2-fold decrease in substrate affinity for formate and 3-fold decrease in substrate affinity for NAD compared to the wild-type; when associated with A-287. 93-fold decrease in substrate affinity for formate and 2700-fold decrease in substrate affinity for NAD, and a significant reduction in catalytic activity compared to the wild-type; when associated with A-119.; mutation to Q: 10-fold decrease in substrate affinity for formate and significant reduction in the catalytic activity compared to the wild-type.
K328 (≠ R352) mutation to V: A 75% increase in substrate affinity for formate after 2 weeks and a 50% increase in affinity for NAD. However, after 4 months the affinity for formate increases 7-fold and affinity for NAD increases by 2 thirds. Retains 70% of residual activity after incubation for 20 minutes at a thermal inactivation temperature of 55 degrees Celsius in samples stored for 2 weeks compared to wild-type which loses 50% of its activity at 55 degrees Celsius.

Sites not aligning to the query:

360 K→A: Exhibits no change in substrate affinity for formate, but shows a 4-fold decrease in substrate affinity for NAD implying that L-360 side chain forms strong interactions with the cofactor. A higher reaction rate is observed at an acidic and basic pH values.

Q08911 Formate dehydrogenase 1; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
50% identity, 82% coverage: 48:377/401 of query aligns to 19:368/376 of Q08911

query
sites
Q08911

E

E

P

Q

G

E

A

K

L

L

G

G

S

C

V

I

S

E

G

N

E

E

L

L

G

G

L

I

R

R

K

N

F

F

L

I

E

E

G

E

Q

Q

G

G

H

Y

T

E

L

L

V

V

V

T

T

T

S

I

D

D

K

K

D

D

-

P

G

E

P

P

D

T

S

S

V

T

F

V

E

D

R

R

E

E

L

L

V

K

D

D

A

A

E

E

I

I

V

V

I

I

S

T

Q

T

P

P

F

F

W

F

P

P

A

A

Y

Y

L

I

T

S

A

R

E

N

R

R

I

I

V

A

K

E

A

A

A

P

R

N

L

L

K

K

L

L

A

C

I

V

T

T

A

A

G

G

I

V

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

E

A

A

I

N

D

E

R

R

G

K

I

I

T

T

V

V

A

T

E

E

V

V

T

T

Y

G

C

S

N

N

S

V

I

V

S

S

V

V

S

A

E

E

H

H

V

V

V

M

M

A

M

T

I

I

L

L

S

V

L

L

A

I

R

R

N

N

Y

Y

I

N

P

G

S

G

Y

H

Q

Q

W

Q

V

A

V

I

K

N

G

G

G

E

W

W

N

D

V

I

A

A

D

G

C

V

V

A

A

K

R

N

S

E

Y

Y

D

D

I

L

E

E

G

D

M

K

D

I

I

I

G

S

T

T

V

V

G

G

A

A

G

G

R

R

I

I

G

G

T

Y

A

R

V

V

L

L

R

E

R

R

L

L

K

V

P

A

F

F

D

N

-

P

V

K

K

K

L

L

H

L

Y

Y

T

Y

D
|
D

R
x
Y

H

Q

R

E

L

L

P

P

D

A

E

E

V

A

A

I

K

N

E

R

L

L

G

N

-

E

-

A

-

S

-

K

-

L

-

F

-

N

-

G

-

R

-

G

-

D

-

I

V

V

T

Q

F

R

H

V

Q

E

T

K

A

L

A

E

E

D

M

M

V

V

P

A

V

Q

C

S

D

D

V

V

T

T

I

I

N

N

A

C

P

P

L

L

H

H

P

K

E

D

T

S

E

R

N

G

L

L

F

F

N

N

E

K

A

K

M

L

I

I

G

S

K

H

M

M

K

K

R

D

G

G

A

A

Y

Y

L

L

V

V

N

N

T

T

A

A

R

R

G

G

K

A

I

I

C

C

N

V

R

A

D

E

A

D

V

V

A

A

R

E

A

A

L

V

E

K

S

S

G

G

Q

K

L

L

A

A

G

G

Y

Y

A

G

G

G

D

D

V

V

W

W

F

D

P

K

Q

Q

P

P

A

A

P

P

K

K

D

D

H

H

P

P

W

W

R

R

S

T

M

M

P

D

H

N

-

K

-

D

-

H

-

V

-

G

H

N

G

A

M

M

T

T

P

V

H

H

I

I

S

S

G

G

S

T

S

S

L

L

S

D

A

A

Q

Q

A

K

R

R

Y

Y

A

A

A

Q

G

G

T

V

R

K

E

N

I

I

L

L

E

N

C

S

W

Y

F

F

E

S

G

K

R

K

-

F

P

D

I

Y

R

R

E

P

E

Q

Y

D

L

I

I

I

V

V

S

Q

G

N

G

G

K

S

L

Y

A

A

DY 197:198 (≠ DR 224:225) mutation to AR: Shifts the coenzyme preference of the enzyme from NAD(+) to NADP(+).

Query Sequence

>SMa0478 FitnessBrowser__Smeli:SMa0478
MEMAKVACVLYDDPVDGYPTAYARDGLPTLERYPGGQTLPTPKAIDFEPGALLGSVSGEL
GLRKFLEGQGHTLVVTSDKDGPDSVFERELVDAEIVISQPFWPAYLTAERIVKAARLKLA
ITAGIGSDHVDLQAAIDRGITVAEVTYCNSISVSEHVVMMILSLARNYIPSYQWVVKGGW
NVADCVARSYDIEGMDIGTVGAGRIGTAVLRRLKPFDVKLHYTDRHRLPDEVAKELGVTF
HQTAAEMVPVCDVVTINAPLHPETENLFNEAMIGKMKRGAYLVNTARGKICNRDAVARAL
ESGQLAGYAGDVWFPQPAPKDHPWRSMPHHGMTPHISGSSLSAQARYAAGTREILECWFE
GRPIREEYLIVSGGKLAGAGAHSYSAGDATRGSEEAAHFKT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory