SitesBLAST
Comparing SMa1466 FitnessBrowser__Smeli:SMa1466 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7ahhC Opua inhibited inward-facing, sbd docked (see paper)
33% identity, 98% coverage: 2:366/371 of query aligns to 3:381/382 of 7ahhC
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: L275 (≠ F262), S297 (≠ A284), S298 (≠ A285)
- binding phosphoaminophosphonic acid-adenylate ester: F12 (≠ Y11), T39 (vs. gap), V40 (≠ I19), G41 (≠ A20), G62 (= G41), G64 (= G43), K65 (= K44), D187 (= D164), E188 (= E165)
7aheC Opua inhibited inward facing (see paper)
33% identity, 98% coverage: 2:366/371 of query aligns to 3:381/382 of 7aheC
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: L275 (≠ F262), S297 (≠ A284), S298 (≠ A285)
7ahdC Opua (e190q) occluded (see paper)
40% identity, 64% coverage: 2:237/371 of query aligns to 3:260/260 of 7ahdC
- binding adenosine-5'-triphosphate: F12 (≠ Y11), T39 (vs. gap), S61 (= S40), G62 (= G41), G64 (= G43), K65 (= K44), S66 (≠ T45), T67 (= T46), Q111 (= Q86), K161 (= K138), Q162 (≠ E139), S164 (= S141), G166 (= G143), M167 (≠ Q144), Q188 (≠ E165), H221 (= H198)
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
40% identity, 65% coverage: 2:242/371 of query aligns to 7:245/375 of 2d62A
1g291 Malk (see paper)
41% identity, 65% coverage: 2:242/371 of query aligns to 4:242/372 of 1g291
- binding magnesium ion: D69 (= D73), E71 (≠ I75), K72 (= K76), K79 (≠ L77), D80 (≠ R78)
- binding pyrophosphate 2-: S38 (= S40), G39 (= G41), C40 (= C42), G41 (= G43), K42 (= K44), T43 (= T45), T44 (= T46)
Sites not aligning to the query:
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
41% identity, 65% coverage: 2:242/371 of query aligns to 7:231/353 of 1vciA
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
37% identity, 65% coverage: 2:244/371 of query aligns to 3:237/374 of 2awnB
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
37% identity, 65% coverage: 2:244/371 of query aligns to 4:238/371 of P68187
- A85 (≠ G89) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ D110) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ A118) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L121) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (= E123) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ D130) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G143) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D164) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ S234) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
37% identity, 65% coverage: 2:244/371 of query aligns to 3:237/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y11), S37 (= S40), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), Q81 (= Q86), R128 (= R135), A132 (≠ E139), S134 (= S141), G136 (= G143), Q137 (= Q144), E158 (= E165), H191 (= H198)
- binding magnesium ion: S42 (≠ T45), Q81 (= Q86)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
37% identity, 65% coverage: 2:244/371 of query aligns to 3:237/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ Y11), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), R128 (= R135), S134 (= S141), Q137 (= Q144)
- binding beryllium trifluoride ion: S37 (= S40), G38 (= G41), K41 (= K44), Q81 (= Q86), S134 (= S141), G136 (= G143), H191 (= H198)
- binding magnesium ion: S42 (≠ T45), Q81 (= Q86)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
37% identity, 65% coverage: 2:244/371 of query aligns to 3:237/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ Y11), V17 (≠ A20), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), R128 (= R135), A132 (≠ E139), S134 (= S141), Q137 (= Q144)
- binding tetrafluoroaluminate ion: S37 (= S40), G38 (= G41), K41 (= K44), Q81 (= Q86), S134 (= S141), G135 (= G142), G136 (= G143), E158 (= E165), H191 (= H198)
- binding magnesium ion: S42 (≠ T45), Q81 (= Q86)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
37% identity, 65% coverage: 2:244/371 of query aligns to 3:237/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ Y11), V17 (≠ A20), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), R128 (= R135), A132 (≠ E139), S134 (= S141), Q137 (= Q144)
- binding magnesium ion: S42 (≠ T45), Q81 (= Q86)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
38% identity, 65% coverage: 2:244/371 of query aligns to 4:238/369 of P19566
- L86 (= L90) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P166) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D171) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
37% identity, 65% coverage: 2:244/371 of query aligns to 1:235/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ Y11), S35 (= S40), G36 (= G41), C37 (= C42), G38 (= G43), K39 (= K44), S40 (≠ T45), T41 (= T46), R126 (= R135), A130 (≠ E139), S132 (= S141), G134 (= G143), Q135 (= Q144)
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
38% identity, 65% coverage: 1:240/371 of query aligns to 2:236/241 of 4u00A
P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 65% coverage: 1:242/371 of query aligns to 1:243/343 of P30750
- 40:46 (vs. 40:46, 71% identical) binding
- E166 (= E165) mutation to Q: Exhibits little ATPase activity.
Sites not aligning to the query:
- 278:283 binding
- 295 N→A: Reduces the binding of L-methionine to undetectable levels.
- 295:296 binding
3d31A Modbc from methanosarcina acetivorans (see paper)
33% identity, 73% coverage: 1:271/371 of query aligns to 1:254/348 of 3d31A
Sites not aligning to the query:
6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
34% identity, 65% coverage: 1:242/371 of query aligns to 2:244/344 of 6cvlD
- binding phosphothiophosphoric acid-adenylate ester: F12 (≠ Y11), Q14 (≠ P13), I19 (≠ L18), S41 (= S40), G42 (= G41), A43 (≠ C42), G44 (= G43), K45 (= K44), S46 (≠ T45), T47 (= T46), N141 (≠ E139), S143 (= S141), Q146 (= Q144), H200 (= H198)
3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
34% identity, 65% coverage: 1:242/371 of query aligns to 2:244/344 of 3tuzC
Sites not aligning to the query:
3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
34% identity, 65% coverage: 1:242/371 of query aligns to 2:244/344 of 3tuiC
Query Sequence
>SMa1466 FitnessBrowser__Smeli:SMa1466
MIRLENLTKHYGPAHDPLIAVDNVSLDLPTGEICVLLGPSGCGKTTTMKMINRLIQPTSG
KVFINGKDTSTIDPIKLRRTIGYVIQQIGLFPNKTVEENICVVPDLLGWDRRKSRARAKE
LLELVGLQPDLFLKRYPKELSGGQQQRVGVLRALAADPPVMLMDEPFGAIDPINREAIQE
EFLKMQREIRKTIIFVSHDLDEAVKMADKIAIFRSGRLEQYAAPDELLARPANSFIEDFL
GSDRALKRLRLVSVRDAMETGFITVRSSDSVEHALERMRSSRSAAVFVLNADGAPQSLLS
EQVAELRSGTVGDHAEPVKSAVPTTGDLRQAVSIMFAHDMPLLPCVDEGGRMAGVMSYRS
IVHYLAHGAKA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory