SitesBLAST

Q0QLF2 Nicotinate dehydrogenase large molybdopterin subunit; NDH; Nicotinic acid hydroxylase large molybdopterin subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see 2 papers)
23% identity, 48% coverage: 205:564/747 of query aligns to 4:381/425 of Q0QLF2

query
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Q0QLF2

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Q208 (= Q385) binding
GFG 238:240 (≠ FFG 415:417) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2:425 modified: mature protein, Nicotinate dehydrogenase large molybdopterin subunit

4zohA Crystal structure of glyceraldehyde oxidoreductase (see paper)
28% identity, 18% coverage: 608:738/747 of query aligns to 561:696/701 of 4zohA

query
sites
4zohA

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active site: E668 (= E710), A669 (≠ P711)
binding pterin cytosine dinucleotide: I594 (≠ V643), N595 (= N644), L598 (≠ I647), Q602 (= Q651), K664 (vs. gap), G665 (= G707), I666 (= I708), G667 (= G709), E668 (= E710)

Sites not aligning to the query:

active site: 186, 219, 298, 300, 304, 332
binding pterin cytosine dinucleotide: 213, 214, 215, 332, 442, 443, 444, 446, 482, 484, 486, 487

3hrdB Crystal structure of nicotinate dehydrogenase (see paper)
32% identity, 17% coverage: 613:742/747 of query aligns to 183:321/330 of 3hrdB

query
sites
3hrdB

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active site: E289 (= E710), P290 (= P711)
binding pterin cytosine dinucleotide: I215 (≠ V643), N216 (= N644), M219 (≠ I647), V220 (≠ I648), Q223 (= Q651), K285 (vs. gap), G286 (= G707), V287 (≠ I708), G288 (= G709), E289 (= E710)

Sites not aligning to the query:

binding pterin cytosine dinucleotide: 45, 46, 47, 48, 49, 85, 87, 89, 90
binding nicotinic acid: 17, 18

Q0QLF1 Nicotinate dehydrogenase medium molybdopterin subunit; NDH; Nicotinic acid hydroxylase medium molybdopterin subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
32% identity, 17% coverage: 613:742/747 of query aligns to 183:321/330 of Q0QLF1

query
sites
Q0QLF1

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211:223 (vs. 639:651, 31% identical) binding
AKGVGE 284:289 (≠ --GIGE 707:710) binding

Sites not aligning to the query:

45:49 binding
85:90 binding

O54754 Aldehyde oxidase 1; Azaheterocycle hydroxylase 1; Retinal oxidase; EC 1.2.3.1; EC 1.17.3.- from Mus musculus (Mouse) (see paper)
25% identity, 36% coverage: 209:479/747 of query aligns to 579:864/1333 of O54754

query
sites
O54754

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A

V

T

A

L

G

K

I

L

A

S

P

A

E

N

K

K

V

V

T

M

I

C

H

H

S

V

P

R

M

R

L

V

G

G

F

A

F

F

G

G

R

G

H

K

F

-

L
x
V

Y

G

Q

K

T

T

A

S

N

I

P

L

F

A

P

A

Q

I

A

T

I

A

L

F

L

A

A

A

K

S

A

K

V

H

A

G

R

R

P

A

V

V

K

R

L

C

I

I

W

L

S

E

R

R

E

G

E

E

E

D

F

M

L

L

R

I

D

T

T

G

L

G

R

R

P

H

M

P

G

Y

A

L

V

G

R

K

F

Y

R

K

A

A

G

G

L

F

D

M

A

N

E

E

G

G

L

R

P

I

V

L

A

A

L

L

E

D

V806 (≠ L421) mutation to E: Decreases substrate affinity and activity on benzaldehyde, phthalazine and acetaldehyde, while increases affinity for more hydrophobic aldehydes like retinal. Abolishes catalytic activity; when associated with R-884.

Sites not aligning to the query:

884 M→R: Abolishes catalytic activity on phthalazine and acetaldehyde. Decreases catalytic efficiency on benzaldehyde and retinal. Abolishes catalytic activity; when associated with E-806.
1265 E→Q: Abolishes catalytic activity.

Q8GUQ8 Xanthine dehydrogenase 1; AtXDH1; EC 1.17.1.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
24% identity, 55% coverage: 209:617/747 of query aligns to 598:1040/1361 of Q8GUQ8

query
sites
Q8GUQ8

I

V

G

G

K

S

P

S

V

E

A

V

R

H

L

L

D

S

V

A

R

R

D

M

K

Q

S

V

T

T

G

G

K

E

A

A

R

E

Y

Y

A

T

I

D

D

D

L

T

K

P

V

V

D

P

-

P

R

N

M

T

L

L

H

H

A

A

-

F

-

V

-

L

-

S

-

K

V

V

Q

P

H

H

S

A

P

R

R

I

L

L

G

S

G

I

E

D

P

D

G

S

A

A

L

A

Q

K

N

S

E

S

A

S

D

G

V

F

R

V

G

G

M

L

-

F

-

L

-

A

-

K

-

D

-

I

P

P

G

G

V

D

H

N

S

M

I

I

H

G

-

P

-

I

-

V

-

P

-

D

-

E

-

L

-

F

-

A

-

T

-

D

-

V

S

T

L

C

P

V

G

G

T

Q

V

V

-

I

-

G

A

V

V

V

A

A

D

D

S

T

W

H

W

E

R

N

A

A

R

K

M

T

A

A

E

G

A

K

L

V

Q

D

V

V

T

R

W

Y

T

E

E

E

P

-

T

-

R

-

G

-

T

-

A

-

H

-

V

-

M

L

P

P

A

A

D

I

F

L

S

S

T

I

E

K

A

E

H

A

M

I

A

N

M

A

L

K

K

S

A

F

T

H

P

P

G

N

E

T

G

E

V

K

A

R

Y

L

E

R

T

K

V

-

G

G

N

D

A

V

A

E

T

L

A

C

L

F

-

Q

-

S

G

G

D

Q

A

C

A

D

R

R

V

V

V

I

E

E

A

G

T

E

Y

V

D

Q

A

M

P

G

Y

G

L

Q

V

E

H

H

G

F

Q

Y

L

L

E

E

P

P

P

N

S

G

A

S

L

L

A

V

R

-

W

W

N

T

D

V

D

D

G

G

-

G

-

S

S

E

L

V

D

H

L

M

W

I

V

S

P

S

N

T

Q

Q

A

A

P

P

E

Q

M

K

F

H

Q

Q

A

K

E

Y

A

V

A

S

K

H

V

V

A

L

G

G

I

L

A

P

P

M

E

S

K

K

V

V

T

V

I

C

H

K

S

T

P

K

M

R

L

I

G

G

F

G

F

F

G

G

-

G

-

K

-
x
E

-

T

R

R

H

S

F

A

L

F

Y

I

Q

A

T

A

A

A

N

A

P

S

F

V

P

P

Q

S

A

Y

I

L

L

L

L

-

A

-

K

-

A

-

V

-

A

N

R

R

P

P

V

V

K

K

L

L

I

I

W

L

S

D

R

R

E

D

E

V

E

D

F

M

L

M

R

I

D

T

T

G

L

H

R

R

P

H

M

S

G

F

A

L

V

G

R

K

F

Y

R

K

A

V

G

G

L

F

D

T

A

N

E

E

G

G

L

K

P

I

V

L

A

A

L

L

E

D

A

L

V

E

A

I

V

Y

G

N

E

N

G

G

P

-

T

-

G

-

R

-

W

-

F

-

G

G

R

N

Q

S

P

L

D

D

K

L

V

S

D

L

S

S

S

V

S

L

V

E

E
x
R

G

A

I

M

-

F

-

H

A

S

G

D

K

N

V

V

Y

Y

A

E

I

I

P

P

N

H

-

V

R

R

H

I

I

V

G

G

Q

N

V

V

H

C

V

F

D

T

D

N

-

F

P

P

A

S

I

N

I

T

G

A

F

F

W

-

R

R

S

G

V

F

G

G

H

G

S

P

M

Q

N

G

D

M

F

L

F

I

Y

T

E

E

T

N

F

W

F

I

D

Q

E

R

M

I

A

A

D

A

A

E

G

L

Q

N

Q

K

D

S

P

P

Y

E

E

E

L

I

R

K

R

E

R

M

L

N

L

F

-

Q

-

V

-

E

-

G

-

S

-

V

-

T

-

H

-

Y

A

C

D

Q

S

T

P

L

R

Q

H

H

R

C

T

T

L

L

L

H

E

Q

A

L

V

W

A

K

E

E

L

L

-

K

-

V

S

S

G

C

G

N

W

F

R

L

R

K

G

A

P

R

F

R

I

E

A

A

D

D

D

E

G

F

T

N

R

S

-

H

-

N

-

R

-

W

R

K

A

K

R

R

G

G

V

V

A

A

M

M

A

V

-

P

S

T

P

K

F

F

G

G

S

I

E

S

V

F

A

T

T

T

E831 (vs. gap) mutation to A: Loss of activity.
R909 (≠ E505) mutation to A: Decreases activity 12-fold.

Sites not aligning to the query:

364 W→A: Decreases activity 8-fold.
421 Y→A: Decreases activity 4-fold.
1297 E→A: Decreases activity 40-fold.

Q7G9P4 Abscisic-aldehyde oxidase; Aldehyde oxidase 3; AO-3; AtAO-3; AtAO4; Indole-3-acetaldehyde oxidase; IAA oxidase; EC 1.2.3.14; EC 1.2.3.7 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 20% coverage: 333:479/747 of query aligns to 716:862/1332 of Q7G9P4

query
sites
Q7G9P4

E

E

T

P

V

V

G

G

N

D

A

V

A

I

T

K

A

G

L

M

G

E

D

E

A

A

A

E

R

R

V

K

V

I

-

I

-

S

E

E

A

L

T

R

Y

L

D

G

A

S

P

Q

Y

Y

L

F

V

F

H

Y

G

-

Q

-

L

M

E

E

P

P

P

Q

S

T

A

A

L

L

A

A

R

L

W

P

N

D

D

E

D

D

G

N

S

C

L

V

D

K

L

V

W

F

V

S

P

S

N

S

Q

Q

A

A

P

P

E

E

M

Y

F

V

Q

H

A

S

E

V

A

I

A

A

K

T

V

C

A

L

G

G

I

I

A

Q

P

E

E

H

K

N

V

V

T

R

I

V

H

I

S

T

P

R

M

R

L

V

G

G

F

G

F

F

G

G

R

G

H

K

F

A

L

V

Y

-

Q

-

T

K

A

S

N

M

P

P

F

V

P

A

Q

T

A

A

I

C

L

A

L

L

-

G

A

A

K

Y

A

K

V
x
L

A
x
Q

R
|
R

P
|
P

V
|
V

K
|
K

L

M

I

F

W

L

S

N

R

R

E

K

E

T

E

D

F

M

L

I

R

M

D

A

T

G

L

G

R

R

P

H

M

P

G

M

A

K

V

I

R

N

F

Y

R

N

A

V

G

G

L

F

D

R

A

S

E

D

G

G

L

K

P

L

V

T

A

A

L

L

E

E

LQRPVK 821:826 (≠ VARPVK 438:443) mutation to WDLDQ: In aao3-3; wilty phenotype in rosette leaves, reduced ABA levels, reduced dormancy, abnormal water loss and abnormal response to water deficit.

1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
28% identity, 19% coverage: 595:736/747 of query aligns to 751:895/906 of 1dgjA

query
sites
1dgjA

A

A

D

K

D

D

G

C

T

D

R

A

R

K

A

G

R

Q

G

G

V

S

A

P

M

F

A

A

S

C

P

Y

F

M

G

Y

S

G

E

L

V

F

A

L

T

T

I

E

A

V

E

A

V

V

S

E

L

V

R

A

S

T

G

G

E

K

V

A

V

T

V

V

H

E

D

K

V

M

W

V

V

C

A

V

I

A

D

D

P

I

G

G

S

K

I

I

V
x
C

N
|
N

P

K

A

L

I
x
V

E

D

A

G

Q
|
Q

V

I

N

Y

S

G

A

G

V

L

A

A

L

Q

G

G

L

V

S

G

S

L

A

A

L

L

L

S

E

E

E

D

V

-

Y

Y

V

E

D

D

G

-

M

L

P

K

Q

K

A

H

R

S

N

T

Y

M

D

G

G

G

Y

A

P

G

I

I

-

P

-

S

-

I

-

K

L

M

T

I

P

P

D

D

R

D

M

I

P

E

R

I

V

V

H

Y

V

V

R

E

I

T

V

P

E

R

S

K

G

D

A

G

P

P

M

F

G

G

-

A

-
x
S

G
|
G

I
x
V

G
|
G

E
|
E

P
x
M

G

P

L

L

P

T

G

A

V

P

P

H

P

A

A

A

I

I

A

I

N

N

A

G

V

I

S

Y

V

N

L

A

A

C

G

G

R

A

R

R

V

V

R

R

S

H

L

L

P

P

active site: E869 (= E710), M870 (≠ P711)
binding molybdenum (iv)oxide: E869 (= E710)
binding pterin cytosine dinucleotide: C799 (≠ V643), N800 (= N644), V803 (≠ I647), V804 (≠ I648), Q807 (= Q651), S865 (vs. gap), G866 (= G707), V867 (≠ I708), G868 (= G709), E869 (= E710)

Sites not aligning to the query:

active site: 391, 427, 503, 507, 535
binding molybdenum (iv)oxide: 424, 535, 698
binding fe2/s2 (inorganic) cluster: 38, 39, 40, 41, 43, 44, 45, 46, 48, 58, 60, 100, 101, 103, 137, 139
binding pterin cytosine dinucleotide: 99, 139, 423, 424, 535, 652, 655, 656, 657, 658, 697, 698, 700, 702, 703

6a7xB Rat xanthine oxidoreductase, d428a variant, NAD bound form
22% identity, 39% coverage: 195:486/747 of query aligns to 525:838/1291 of 6a7xB

query
sites
6a7xB

P

P

T

A

N

N

V

V

V

Q

L

L

-

F

-

Q

-

E

-

V

-

P

R

K

D

D

R

Q

A

S

D

E

F

E

R

D

W

M

I

V

G

G

K

R

P

P

V

L

A

P

R

H

L

L

D

A

V

A

R

N

D

M

K

Q

S

A

T

S

G

G

K

E

A

A

R

V

Y

Y

A

C

I

D

D

D

L

I

-

P

K

R

V

Y

D

E

R

N

M

E

L

L

H

S

A

L

A

R

V

L

Q

V

H

T

S

S

P

T

R

R

L

A

G

H

G

A

E

K

-

I

-

T

-

S

-

I

-

D

-

T

-

S

-

E

-

A

-

K

-

V

P

P

G

G

-

F

-

V

-

C

-

F

-

L

-

T

-

A

-

E

-

D

-

V

-

P

-

N

-

S

-

N

-

A

-

T

A

G

L

L

Q

F

N

N

E

D

A

E

D

T

V

V

R

F

G

A

M

K

P

D

G

E

V

V

H

T

S

C

I

V

H

G

S

H

L

I

P

I

G

G

T

-

V

-

A

A

V

V

A

A

D

D

S

T

W

P

W

E

R

H

A

A

R

Q

M

R

A

A

E

R

A

G

L

V

Q

K

V

I

T

T

W

Y

T

E

E

D

P

-

T

-

R

-

G

-

T

-

A

-

H

-

V

-

M

L

P

P

A

A

D

I

F

I

S

T

T

I

E

Q

A

D

H

A

M

I

A

N

M

N

L

-

K

N

A

S

T

F

P

Y

G

G

E

S

G

E

V

I

A

K

Y

I

E

E

T

K

V

-

G

G

N

D

A

L

A

K

T

K

A

G

L

F

G

S

D

E

A

A

A

D

R

N

V

V

E

S

A

G

T

E

Y

L

D

Y

A

I

P

G

Y

G

L

Q

V

E

H

H

G

F

Q

Y

L
|
L

E

E

P

T

P

N

S

C

A

T

L

I

A

A

R

V

-

P

W

K

N

G

D

E

D

A

G

G

S

E

L

M

D

E

L

L

W

F

V

V

P

S

N

T

Q
|
Q

A

N

P

T

E

M

M

K

F

T

Q

Q

A

S

E

F

A

V

A

A

K

K

V

M

A

L

G

G

I

V

A

P

P

D

E

N

K

R

V

I

T

V

I

V

H

R

S

V

P

K

M

R

L

M

G

G

F

G

F

F

G

G

-

G

-

K

-
x
E

-

T

R

R

H

S

F

T

L

V

Y

V

Q

S

T

T

A

A

N

-

P

-

F

-

P

-

Q

-

A

L

I

A

L

L

L

A

A

A

K

H

A

K

V

T

A

G

R

R

P

P

V

V

K

R

L

C

I

M

W

L

S

D

R

R

E

D

E

E

E

D

F

M

L

L

R

I

D

T

T

G

L

G

R
|
R

P
x
H

M

P

G

F

A

L

V

A

R

K

F

Y

R

K

A

V

G

G

L

F

D

M

A

K

E

T

G

G

L

T

P

V

V

V

A

A

L

L

E

E

A

V

V

A

A

H

V

F

G

S

E

N

G

G

active site: Q738 (= Q385), E773 (vs. gap)
binding bicarbonate ion: R810 (= R458), H811 (≠ P459)
binding fe2/s2 (inorganic) cluster: L715 (= L363)
binding uric acid: E773 (vs. gap)

Sites not aligning to the query:

active site: 851, 855, 883, 1231, 1232
binding bicarbonate ion: 880, 881, 885, 886, 889
binding flavin-adenine dinucleotide: 44, 227, 228, 229, 230, 231, 232, 233, 234, 307, 312, 317, 320, 321, 323, 324, 330, 373, 374
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 71, 109, 110, 111, 113, 145, 147
binding nicotinamide-adenine-dinucleotide: 327, 363, 364, 428, 430, 431, 471, 478
binding uric acid: 851, 885, 980, 981, 1049, 1050, 1232

2e1qA Crystal structure of human xanthine oxidoreductase mutant, glu803val (see paper)
26% identity, 27% coverage: 278:479/747 of query aligns to 644:835/1307 of 2e1qA

query
sites
2e1qA

A

A

V

V

A

A

D

D

S

T

W

P

W

E

R

H

A

T

R

Q

M

R

A

A

E

Q

A

G

L

V

Q

K

V

I

T

T

W

Y

T

E

E

E

P

-

T

-

R

-

G

-

T

-

A

-

H

-

V

-

M

L

P

P

A

A

D

I

F

I

S

T

T

I

E

E

A

D

H

A

M

I

A

K

M

N

L

-

K

N

A

S

T

F

P

Y

G

G

E

P

G

E

V

L

A

K

Y

I

E

E

T

K

V

-

G

G

N

D

A

L

A

K

T

K

A

G

L

F

G

S

D

E

A

A

A

D

R

N

V

V

E

S

A

G

T

E

Y

I

D

Y

A

I

P

G

Y

G

L

Q

V
x
E

H
|
H

G

F

Q
x
Y

L
|
L

E

E

P

T

P

H

S

C

A

T

L

I

A

A

R

V

-

P

W

K

N

G

D

E

D

A

G

G

S

E

L

M

D

E

L

L

W

F

V

V

P

S

N
x
T

Q
|
Q

A

N

P

T

E

M

M

K

F
x
T

Q

Q

A

S

E

F

A

V

A

A

K

K

V

M

A

L

G

G

I

V

A

P

P

A

E

N

K

R

V

I

T

V

I

V

H

R

S

V

P

K

M

R

L

M

G

G

F

G

F
|
F

G
|
G

R

G

H

K

F
x
V

L

T

Y

R

Q
x
S

T
|
T

A

V

N

V

P
x
S

F

T

P

A

Q

V

A

A

I

-

L

L

L

A

A

A

K

Y

A

K

V

T

A

G

R

R

P

P

V

V

K

R

L

C

I

M

W

L

S

D

R

R

E

D

E

E

E

D

F

M

L

L

R

I

D
x
T

T
x
G

L

G

R
|
R

P
x
H

M

P

G

F

A

L

V

A

R

R

F

Y

R

K

A

V

G

G

L

F

D

M

A

K

E

T

G

G

L

T

P

V

V

V

A

A

L

L

E

E

active site: Q742 (= Q385), V777 (≠ F420)
binding bicarbonate ion: R814 (= R458), H815 (≠ P459)
binding calcium ion: E715 (≠ V359), H716 (= H360), Y718 (≠ Q362), T741 (≠ N384), T747 (≠ F390), S780 (≠ Q423), T781 (= T424), S784 (≠ P427), T811 (≠ D455), G812 (≠ T456)
binding fe2/s2 (inorganic) cluster: L719 (= L363)
binding hydroxy(dioxo)molybdenum: F773 (= F416), G774 (= G417)

Sites not aligning to the query:

active site: 855, 859, 887, 1235, 1236
binding bicarbonate ion: 852, 886, 889, 890, 893
binding flavin-adenine dinucleotide: 43, 44, 230, 231, 232, 233, 234, 235, 236, 237, 238, 311, 312, 316, 320, 321, 324, 325, 327, 328, 333, 334, 377, 378
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 71, 110, 111, 112, 114, 146, 148
binding hydroxy(dioxo)molybdenum: 887, 1053, 1054, 1236

Query Sequence

>SMa1488 FitnessBrowser__Smeli:SMa1488
MNDMTPVTSEITASEIGKPFKLPRRGFLGASLGALVLGVTLPAGRARAQAAAAAITPGTR
ISAFLEILPNETVLFRSAFIEGGQGIFTAMAQIVGEELDVDPMQFVVEGAPPGPDYLLTG
GGRFTGGSMSVRMSYDAMRKLGASARHMLIQAAAVRLRVPVSELSTEPGRVVHGASGRTL
PYGEIADAAAGLPLPTNVVLRDRADFRWIGKPVARLDVRDKSTGKARYAIDLKVDRMLHA
AVQHSPRLGGEPGALQNEADVRGMPGVHSIHSLPGTVAVVADSWWRARMAAEALQVTWTE
PTRGTAHVMPADFSTEAHMAMLKATPGEGVAYETVGNAATALGDAARVVEATYDAPYLVH
GQLEPPSALARWNDDGSLDLWVPNQAPEMFQAEAAKVAGIAPEKVTIHSPMLGGFFGRHF
LYQTANPFPQAILLAKAVARPVKLIWSREEEFLRDTLRPMGAVRFRAGLDAEGLPVALEA
VAVGEGPTGRWFGRQPDKVDSSSVEGIAGKVYAIPNRHIGQVHVDDPAIIGFWRSVGHSM
NDFFYETFFDEMADAGQQDPYELRRRLLADSPRHRTLLEAVAELSGGWRRGPFIADDGTR
RARGVAMASPFGSEVATIAEVSLRSGEVVVHDVWVAIDPGSIVNPAIIEAQVNSAVALGL
SSALLEEVVYVDGMPQARNYDGYPILTPDRMPRVHVRIVESGAPMGGIGEPGLPGVPPAI
ANAVSVLAGRRVRSLPLSKHDFKGVDG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory