SitesBLAST

O32148 (S)-ureidoglycine--glyoxylate transaminase; UGXT; (S)-ureidoglycine--glyoxylate aminotransferase; Purine catabolism protein PucG; EC 2.6.1.112 from Bacillus subtilis (strain 168) (see paper)
38% identity, 99% coverage: 4:369/370 of query aligns to 7:387/416 of O32148

query
sites
O32148

F

L

S

C

P

T

P

P

P

L

R

R

L

T

L

I

M

M

G

T

P

P

G

G

P

P

S

V

N

E

V

V

S

D

P

P

E

R

V

V

L

L

A

R

A

V

Q

M

A

S

R

T

P

P

T

V

I

V

G

G

H
x
Q

L

F

D

D

P

P

S

A

F

F

V

T

G

G

L

I

M

M

D

N

R

E

I

T

K

M

D

E

Q

M

L

L

R

R

L

E

A

L

F

F

R

Q

T

T

D

K

N

N

R

R

V

W

T

A

F

Y

P

P

L

I

S

D

A

G

P

T

A

S

S

R

L

A

A

G

M

I

E

E

M

A

A

V

L

L

V

A

T

S

L

V

L

I

E

E

P

P

G

E

D

D

T

D

A

V

I

L

I

I

A

P

Q

I

N

Y

G

G

V

R

F

F

G

G

G

Y

R

L

M

L

A

T

E

E

I

I

A

A

Q

E

R

R

A

Y

G

G

A

A

E

N

V

V

R

H

L

M

V

L

S

E

V

C

E

E

W

W

G

G

K

T

P

V

V

F

D

D

P

P

E

E

A

D

V

I

R

I

A

R

S

E

I

I

L

K

E

K

A

V

P

-

Q

K

A

P

K

K

L

I

L

V

A

A

F

M

V

V

H

H

A

G

E

E

T

T

S

S

T

T

G

G

V

R

R

I

S

H

D

P

A

L

A

K

S

A

L

I

C

G

A

E

L

A

A

C

R

R

E

T

A

E

G

D

L

A

L

L

S

F

V

I

V

V

D

D

T

A

V

V

T

A

G

T

L

I

G

G

I

C

P

E

V

V

S

K

V

V

D

D

E

E

W

W

Q

K

A

I

D

D

A

A

V

A

Y

I

A

G

G

G

T

T

Q

Q

K
|
K

C

C

L

L

S

S

A

V

P

P

P

S

G

G

L

M

A

A

P

P

I

I

T

T

F

Y

S

N

D

E

R

R

A

V

V

A

S

D

A

V

V

I

K

A

A

A

R

R

K

K

T

K

-

V

-

E

-

R

-

G

-

I

-

A

-

T

-

Q

-

A

-

D

-

R

-

A

-

L

-

S

-

G

-

N

-

R

P

P

I

I

Q

T

S

S

W

N

F

Y

L

F

D

D

L

L

G

S

L

Q

M

L

L

E

G

D

Y

Y

W

W

E

S

G

E

E

R

G

-

A

-

R

R

S

L

Y
x
N

H

H

T

T

A

E

P

A

V

T

N

T

A

M

L

L

Y

Y

G

A

L

L

H

R

E

E

S

G

L

V

S

R

R

L

L

V

L

L

G

E

E

E

G

G

L

L

E

E

T

T

A

R

W

F

A

E

R

R

H

H

R

R

A

H

H

E

D

A

R

A

L

L

V

A

E

A

R

G

L

I

Q

K

G

A

L

M

G

G

I

L

A

R

F

L

V

F

V

G

D

D

K

D

E

S

H

C

R

K

L

M

P

P

Q

V

L

V

N

T

T

C

V

V

W

E

L

I

P

P

E

G

G

G

V

I

K

D

D

G

V

E

P

S

E

V

R

R

R

D

R

M

L

L

D

A

E

Q

F

F

G

G

I

I

E

E

I

I

G

A

G

S

L

F

G

G

P

P

L

L

A

A

G

G

R

K

I

I

W

W

R

R

I

I

G

G

L

T

M

M

G

G

E

Y

T

S

C

C

R

R

I

K

E

E

N

N

V

V

D

L

R

F

L

V

A

L

E

A

A

G

I

L

A

E

A

A

V

V

L

L

Q37 (≠ H34) mutation to H: 5-fold decrease in transamination activity.
K198 (= K196) modified: N6-(pyridoxal phosphate)lysine
N264 (≠ Y246) mutation to S: 9-fold decrease in transamination activity.; mutation to Y: Loss of transamination activity.

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
39% identity, 100% coverage: 1:369/370 of query aligns to 10:379/385 of 5hhyA

query
sites
5hhyA

M

L

N

K

A

P

F

L

S

S

P

I

P

P

P

N

R

Q

L

L

M

L

G

G

P

P

G

G

P

P

S

S

N

N

V

L

S

P

P

P

E

R

V

I

L

M

A

A

Q

G

A

G

R

L

P

Q

T

M

I

I

G

G

H

S

L

M

D

S

P

K

S

D

F

M

V

Y

G

Q

L

I

M

M

D

D

R

E

I

I

K

K

D

E

Q

G

L

I

R

Q

L

Y

A

V

F

F

R

Q

T

T

D

R

N

N

R

P

V

L

T

T

F

L

P

V

L

I

S

S

A

G

P
x
S

A
x
G

S
x
H

L

C

A

A

M

L

E

E

M

A

A

A

L

L

V

V

T

N

L

V

L

L

E

E

P

P

G

G

D

D

T

S

A

F

I

L

I

V

A

G

Q

A

N

N

G

G

V

I

F
x
W

G

G

G

Q

R

R

M

A

A

V

E

D

I

I

A

G

Q

E

R

R

A

I

G

G

A

A

E

R

V

V

R

H

L

P

V

M

S

T

V

K

E

D

W

P

G

G

K

G

P

H

V

Y

D

T

P

L

E

Q

A

E

V

V

R

E

A

E

S

G

I

L

L

A

E

Q

A

H

P

-

Q

K

A

P

K

V

L

L

A

F

F

L

V

T

H

H

A

G

E

E

T
x
S

S

S

T

T

G

G

V

V

R

L

S

Q

D

P

A

L

A

D

S

G

L

F

C

G

A

E

L

L

A

C

R

H

E

R

A

Y

G

K

L

C

L

L

S

L

V

L

V

V

D
|
D

T

S

V
|
V

T

A

G

S

L

L

G

G

I

T

P

P

V

L

S

Y

V

M

D

D

E

R

W

Q

Q

G

A

I

D

D

A

I

V

L

Y

Y

A

S

G

G

T

S

Q
|
Q

K
|
K

C

A

L

L

S

N

A

A

P

P

G

G

L

T

A

S

P

L

I

I

T

S

F

F

S

S

D

D

R

K

A

A

V

K

S

K

A

K

V

M

K

Y

A

S

R

R

K

K

T

T

P

K

I

P

Q

F

S

S

W

F

F

Y

L

L

D

D

L

I

G

K

L

W

M

L

L

A

G

N

Y

F

W

W

E

G

G

C

E

D

G

D

A

Q

-

P

R

R

S

M

Y
|
Y

H

H

T
|
T

A

I

P

P

V

V

N

I

A

S

L

L

Y

Y

G

S

L

L

H

R

E

E

S

S

L

L

S

A

R

L

L

I

L

A

G

E

E

Q

G

G

L

L

E

E

T

N

A

S

W

W

A

R

R

Q

H

H

R

R

A

E

A

A

H

A

D

A

R

Y

L

L

V

H

E

G

R

R

L

L

Q

Q

G

A

L

L

G

G

I

L

A

Q

-

L

F

F

V

V

V

K

D

D

K

P

E

A

H

L

R

R

L

L

P

P

Q

T

L

V

N

T

T

T

V

V

W

A

L

V

P

P

E

A

G

G

V

Y

K

D

D

W

V

R

P

D

E

I

R

V

R

S

R

Y

L

V

L

I

D

D

E

H

F

F

G

D

I

I

E

E

I

I

G

M

G

G

L

L

G

G

P

P

L

S

A

T

G

G

R

K

I

V

W

L

R

R

I

I

G

G

L

L

M

L

G

G

E

C

T

N

C

A

R

T

I

R

E

E

N

N

V

V

D

D

R

R

L

V

A

T

E

E

A

A

I

L

A

R

A

A

V

A

L

L

binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (≠ P67), G77 (≠ A68), H78 (≠ S69), W103 (≠ F94), S153 (≠ T145), D178 (= D170), V180 (= V172), Q203 (= Q195), K204 (= K196), Y255 (= Y246), T258 (= T249)

6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
39% identity, 100% coverage: 1:369/370 of query aligns to 10:379/384 of 6rv0A

query
sites
6rv0A

M

L

N

K

A

P

F

L

S

S

P

I

P

P

P

N

R

Q

L

L

M

L

G

G

P

P

G

G

P

P

S

S

N

N

V

L

S

P

P

P

E

R

V

I

L

M

A

A

Q

G

A

G

R

L

P

Q

T

M

I

I

G

G

H

S

L

M

D

S

P

K

S

D

F

M

V

Y

G

Q

L

I

M

M

D

D

R

E

I

I

K

K

D

E

Q

G

L

I

R

Q

L

Y

A

V

F

F

R

Q

T

T

D

R

N

N

R

P

V

L

T

T

F

L

P

V

L

I

S

S

A

G

P
x
S

A
x
G

S
x
H

L

C

A

A

M

L

E

E

M

A

A

A

L

L

V

V

T

N

L

V

L

L

E

E

P

P

G

G

D

D

T

S

A

F

I

L

I

V

A

G

Q

A

N

N

G

G

V

I

F
x
W

G

G

G

Q

R

R

M

A

A

V

E

D

I

I

A

G

Q

E

R

R

A

I

G

G

A

A

E

R

V

V

R

H

L

P

V

M

S

T

V

K

E

D

W

P

G

G

K

G

P

H

V

Y

D

T

P

L

E

Q

A

E

V

V

R

E

A

E

S

G

I

L

L

A

E

Q

A

H

P

-

Q

K

A

P

K

V

L

L

A

F

F

L

V

T

H

H

A

G

E

E

T

S

S

S

T

T

G

G

V

V

R

L

S

Q

D

P

A

L

A

D

S

G

L

F

C

G

A

E

L

L

A

C

R

H

E

R

A

Y

G

K

L

C

L

L

S

L

V

L

V

V

D
|
D

T

S

V
|
V

T
x
A

G

S

L

L

G

G

I

T

P

P

V

L

S

Y

V

M

D

D

E

R

W

Q

Q

G

A

I

D

D

A

I

V

L

Y

Y

A

S

G

G

T

S

Q

Q

K
|
K

C

A

L

L

S

N

A

A

P

P

G

G

L

T

A

S

P

L

I

I

T

S

F

F

S

S

D

D

R

K

A

A

V

K

S

K

A

K

V

M

K

Y

A

S

R

R

K

K

T

T

P

K

I

P

Q

F

S

S

W

F

F

Y

L

L

D

D

L

I

G

K

L

W

M

L

L

A

G

N

Y

F

W

W

E

G

G

C

E

D

G

D

A

Q

-

P

R

R

S

M

Y

Y

H

H

T

T

A

I

P

P

V

V

N

I

A

S

L

L

Y

Y

G

S

L

L

H

R

E

E

S

S

L

L

S

A

R

L

L

I

L

A

G

E

E

Q

G

G

L

L

E

E

T

N

A

S

W

W

A

R

R

Q

H

H

R

R

A

E

A

A

H

A

D

A

R

Y

L

L

V

H

E

G

R

R

L

L

Q

Q

G

A

L

L

G

G

I

L

A

Q

-

L

F

F

V

V

V

K

D

D

K

P

E

A

H

L

R

R

L

L

P

P

Q

T

L

V

N

T

T

T

V

V

W

A

L

V

P

P

E

A

G

G

V

Y

K

D

D

W

V

R

P

D

E

I

R

V

R

S

R

Y

L

V

L

I

D

D

E

H

F

F

G

D

I

I

E

E

I

I

G

M

G

G

L

L

G

G

P

P

L

S

A

T

G

G

R

K

I

V

W

L

R

R

I

I

G

G

L

L

M

L

G

G

E

C

T

N

C

A

R

T

I

R

E

E

N

N

V

V

D

D

R

R

L

V

A

T

E

E

A

A

I

L

A

R

A

A

V

A

L

L

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S76 (≠ P67), G77 (≠ A68), H78 (≠ S69), W103 (≠ F94), D178 (= D170), V180 (= V172), A181 (≠ T173), K204 (= K196)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
39% identity, 100% coverage: 1:369/370 of query aligns to 15:384/392 of P21549

query
sites
P21549

M

L

N

K

A

P

F

L

S

S

P

I

P

P

P

N

R

Q

L

L

M

L

G

G

P

P

G

G

P

P

S

S

N

N

V

L

S

P

P

P

E
x
R

V

I

L

M

A

A

Q
x
G

A

G

R

L

P

Q

T

M

I

I

G
|
G

H

S

L

M

D

S

P

K

S

D

F

M

V

Y

G

Q

L

I

M

M

D

D

R

E

I

I

K

K

D

E

Q

G

L

I

R

Q

L

Y

A

V

F

F

R

Q

T

T

D

R

N

N

R

P

V

L

T

T

F

L

P

V

L

I

S

S

A

G

P

S

A
x
G

S

H

L

C

A

A

M

L

E

E

M

A

A

A

L

L

V

V

T

N

L

V

L

L

E

E

P

P

G

G

D

D

T

S

A

F

I

L

I

V

A

G

Q

A

N

N

G

G

V

I

F
x
W

G

G

G

Q

R

R

M
x
A

A

V

E

D

I

I

A

G

Q

E

R

R

A

I

G

G

A

A

E

R

V

V

R

H

L

P

V

M

S

T

V

K

E

D

W

P

G

G

K

G

P

H

V

Y

D

T

P

L

E

Q

A

E

V

V

R

E

A

E

S

G

I

L

L

A

E

Q

A

H

P

-

Q

K

A

P

K

V

L
|
L

L

L

A
x
F

F

L

V

T

H

H

A
x
G

E

E

T
x
S

S

S

T

T

G
|
G

V

V

R

L

S

Q

D

P

A
x
L

A

D

S

G

L

F

C
x
G

A

E

L

L

A
x
C

R

H

E

R

A

Y

G

K

L

C

L

L

S

L

V

L

V

V

D
|
D

T

S

V

V

T

A

G
x
S

L

L

G

G

I

T

P

P

V

L

S

Y

V

M

D

D

E

R

W

Q

Q

G

A

I

D

D

A
x
I

V

L

Y

Y

A
x
S

G

G

T

S

Q

Q

K
|
K

C

A

L

L

S

N

A

A

P

P

G

G

L

T

A
x
S

P

L

I

I

T

S

F

F

S

S

D

D

R

K

A

A

V

K

S

K

A

K

V

M

K

Y

A

S

R
|
R

K

K

T

T

P

K

I

P

Q

F

S

S

W

F

F

Y

L

L

D

D

L
x
I

G

K

L

W

M

L

L

A

G

N

Y

F

W

W

E

G

G
x
C

E

D

G

D

A

Q

-

P

R

R

S

M

Y

Y

H

H

T

T

A

I

P

P

V

V

N

I

A

S

L

L

Y

Y

G

S

L

L

H

R

E

E

S

S

L

L

S

A

R

L

L
x
I

L
x
A

G

E

E

Q

G

G

L

L

E

E

T

N

A

S

W

W

A

R

R

Q

H

H

R

R

A

E

A

A

H

A

D

A

R

Y

L

L

V

H

E

G

R

R

L

L

Q

Q

G

A

L

L

G

G

I

L

A

Q

-

L

F

F

V

V

V

K

D

D

K

P

E

A

H

L

R

R

L

L

P

P

Q

T

L

V

N

T

T

T

V

V

W

A

L
x
V

P

P

E

A

G

G

V

Y

K

D

D

W

V

R

P

D

E

I

R

V

R

S

R

Y

L

V

L
x
I

D

D

E

H

F

F

G

D

I

I

E

E

I

I

G

M

G

G

L

L

G

G

P

P

L

S

A

T

G

G

R

K

I

V

W

L

R

R

I

I

G

G

L

L

M

L

G

G

E

C

T

N

C

A

R

T

I

R

E

E

N

N

V

V

D

D

R

R

L

V

A

T

E

E

A

A

I

L

A

R

A

A

V

A

L

L

R36 (≠ E22) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ Q27) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (= G33) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (≠ A68) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ F94) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ M98) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (= L137) to P: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ A139) to I: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in protein destabilization; decreased alanine--glyoxylate aminotransferase activity; no loss of dimerization; mitochondrial mistargeting; dbSNP:rs121908524
G156 (≠ A143) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T145) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (= G148) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (≠ A153) to P: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (≠ C157) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in mitochondrial mistargeting; slight decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; dbSNP:rs121908529
C173 (≠ A160) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D170) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (≠ G174) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (≠ A189) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (≠ A192) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K196) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (≠ A205) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (= R220) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (≠ L231) to T: in HP1; prevalent mutation in the Canary islands; when associated with L-11 and M-340 on the minor AGXT allele; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; no loss of dimerization; partial mitochondrial mistargeting; dbSNP:rs121908525
C253 (≠ G240) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (≠ L265) to T: in dbSNP:rs140992177
A280 (≠ L266) to V: in dbSNP:rs73106685
V326 (≠ L311) to I: in dbSNP:rs115057148
I340 (≠ L325) to M: associated with hyperoxaluria; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: reduction of specific alanine--glyoxylate aminotransferase activity in vitro; causes mitochondrial mistargeting when associated with R-170; dbSNP:rs34116584

1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
39% identity, 100% coverage: 1:369/370 of query aligns to 12:381/387 of 1j04A

query
sites
1j04A

M

L

N

K

A

P

F

L

S

S

P

I

P

P

P

N

R

Q

L

L

M

L

G

G

P
|
P

G

G

P

P

S

S

N

N

V

L

S

P

P

P

E

R

V

I

L

M

A

A

Q

G

A

G

R

L

P

Q

T

M

I

I

G

G

H

S

L

M

D

S

P

K

S

D

F

M

V

Y

G

Q

L

I

M

M

D

D

R

E

I

I

K

K

D

E

Q

G

L

I

R

Q

L

Y

A

V

F

F

R

Q

T

T

D

R

N

N

R

P

V

L

T

T

F

L

P

V

L

I

S

S

A

G

P

S

A

G

S

H

L

C

A

A

M

L

E

E

M

A

A

A

L

L

V

V

T

N

L

V

L

L

E

E

P

P

G

G

D

D

T

S

A

F

I

L

I

V

A

G

Q

A

N

N

G

G

V

I

F

W

G

G

G

Q

R

R

M

A

A

V

E

D

I

I

A

G

Q

E

R

R

A

I

G

G

A

A

E

R

V

V

R

H

L

P

V

M

S

T

V

K

E

D

W

P

G

G

K

G

P

H

V

Y

D

T

P

L

E

Q

A

E

V

V

R

E

A

E

S

G

I

L

L

A

E

Q

A

H

P

-

Q

K

A

P

K

V

L

L

A

F

F

L

V

T

H

H

A

G

E

E

T

S

S

S

T

T

G

G

V

V

R

L

S

Q

D

P

A

L

A

D

S

G

L

F

C

R

A

E

L

L

A

C

R

H

E

R

A

Y

G

K

L

C

L

L

S

L

V

L

V

V

D

D

T

S

V

V

T

A

G

S

L

L

G

G

I

T

P

P

V

L

S

Y

V

M

D

D

E

R

W

Q

Q

G

A

I

D

D

A

I

V

L

Y

Y

A

S

G

G

T

S

Q

Q

K

K

C

A

L

L

S

N

A

A

P

P

G

G

L

T

A

S

P

L

I

I

T

S

F

F

S

S

D

D

R

K

A

A

V

K

S

K

A

K

V

M

K

Y

A

S

R

R

K

K

T

T

P

K

I

P

Q

F

S

S

W

F

F

Y

L

L

D

D

L

I

G

K

L

W

M

L

L

A

G

N

Y

F

W

W

E

G

G

C

E

D

G

D

A

Q

-

P

R

R

S

M

Y

Y

H

H

T

T

A

I

P

P

V

V

N

I

A

S

L

L

Y

Y

G

S

L

L

H

R

E

E

S

S

L

L

S

A

R

L

L

I

L

A

G

E

E

Q

G

G

L

L

E

E

T

N

A

S

W

W

A

R

R

Q

H

H

R

R

A

E

A

A

H

A

D

A

R

Y

L

L

V

H

E

G

R

R

L

L

Q

Q

G

A

L

L

G

G

I

L

A

Q

-

L

F

F

V

V

V

K

D

D

K

P

E

A

H

L

R

R

L

L

P

P

Q

T

L

V

N

T

T

T

V

V

W

A

L

V

P

P

E

A

G

G

V

Y

K

D

D

W

V

R

P

D

E

I

R

V

R

S

R

Y

L

V

L

I

D

D

E

H

F

F

G

D

I

I

E

E

I

I

G

M

G

G

L

L

G

G

P

P

L

S

A

T

G

G

R

K

I

V

W

L

R
|
R

I

I

G

G

L

L

M

L

G

G

E

C

T

N

C

A

R

T

I

R

E

E

N

N

V

V

D

D

R

R

L

V

A

T

E

E

A

A

I

L

A

R

A

A

V

A

L

L

binding (aminooxy)acetic acid: P25 (= P14), R357 (= R345)

Q0IG34 3-hydroxykynurenine transaminase; 3-hydroxykynurenine transaminase and alanine--glyoxylate aminotransferase; Ae-HKT/AGT; Alanine--glyoxylate aminotransferase; EC 2.6.1.63; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
35% identity, 99% coverage: 4:369/370 of query aligns to 3:380/400 of Q0IG34

query
sites
Q0IG34

F

F

S

T

P

P

-

S

-

L

-

R

-

G

-

P

-

L

-

V

-

I

-

P

-

D

R

K

L

I

L

M

M

M

G

G

P

P

G

G

P

P

S

S

N

N

V

C

S

S

P

K

E

R

V

V

L

L

A

A

Q

L

A

N

R

N

P

T

T

C

I

L

G

S

H

N

L

F

D

H

P

D

S

E

F

L

V

F

G

Q

L

V

M

I

D

D

R

E

I

V

K

K

D

D

Q

G

L

L

R

R

L

Y

A

I

F

F

R

Q

T

T

D

E

N

N

R

R

V

T

T

T

F

M

P

C

L

I

S

T

A

G

P
x
S

A
|
A

S
x
H

L

T

A

G

M

M

E

E

M

A

A

L

L

L

V

C

T

N

L

L

E

E

P

E

G

G

D

D

T

I

A

V

I

L

I

I

A

A

Q

N

N

N

G

G

V

I

F

W

G

A

G

E

R

R

M

A

A

I

E

N

I

M

A

A

Q

T

R

R

A

Y

G

G

A

A

E

D

V

V

R

R

L

V

V

L

S

G

V

G

E

P

W

A

G

D

K

K

P

P

V

F

D

S

P

M

E

T

A

D

V

F

R

K

A

K

S

A

I

I

L

-

E

E

A

Q

P

H

Q

R

A

P

K

K

L

C

L

L

A

F

F

V

V

V

H

H

A

G

E

D

T
x
S

S

S

T

S

G

G

V

L

R

L

S

Q

D

P

A

L

A

E

S

G

L

L

C

G

A

K

L

I

A

C

R

H

E

D

A

Y

G

D

L

C

L

L

S

L

V

L

V

V

D

D

T

A

V

V

T

A

G

S

L

L

G

C

G

G

I

V

P

P

V

F

S

Y

V

M

D

D

E

K

W

W

Q

E

A

I

D

D

A

G

V

V

Y

Y

A

T

G

G

T

S

Q
|
Q

K
|
K

C

V

L

L

S

G

A

A

P

P

G

G

L

I

A

T

P

P

I

I

T

S

F

I

S

S

D

P

R

K

A

A

V

L

S

E

A

V

V

I

K

R

A

S

R

R

K

K

T

T

P

P

I

S

Q

K

S

V

W

F

F

Y

L

W

D

D

L

L

G

L

L

I

M

L

L

G

G

N

Y

Y

W

W

E

G

G

C

-

Y

E

D

G

E

A

Q

R

K

S

R

Y
|
Y

H

H

T
|
T

A

V

P

P

V

S

N

N

A

L

L

I

Y

F

G

A

L

L

H

R

E

E

S

A

L

I

S

A

R

Q

L

I

L

A

G

E

E

E

G

G

L

L

E

E

T

P

A

V

W

I

A

R

R

R

H

R

R

Q

A

E

A

C

H

A

D

E

R

Q

L

M

V

Y

E

R

R

G

L

L

Q

Q

G

A

L

M

G

G

I

L

A

E

-

I

F

F

V

V

V

K

D

D

K

P

E

E

H

Y

R

R

L

L

P

P

Q

T

L

V

N

T

T

C

V

I

W

M

L

I

P

P

E

K

G

G

V

V

K

N

D

W

V

W

P

K

E

V

R

S

R

E

R

Y

L

A

L

M

D

N

E

N

F

F

G

S

I

L

E

E

I

I

G

Q

G

G

L

F

G

G

P

P

L

T

A

M

G

G

R

I

I

A

W

W

R

R

I

A

G

G

L

I

M

M

G

G

E

E

T

S

C

S

R

T

I

L

E

Q

N

R

V

V

D

N

R

F

L

Y

A

L

E

Y

A

A

I

F

A

K

A

E

V

S

L

L

SAH 77:79 (≠ PAS 67:69) binding in other chain
S154 (≠ T145) binding in other chain
Q204 (= Q195) binding in other chain
K205 (= K196) modified: N6-(pyridoxal phosphate)lysine
Y256 (= Y246) binding
T259 (= T249) binding

6mfbD Crystal structure of 3-hydroxykynurenine transaminase from aedes aegypti
35% identity, 99% coverage: 4:369/370 of query aligns to 3:380/386 of 6mfbD

query
sites
6mfbD

F

F

S

T

P

P

-

S

-

L

-

R

-

G

-

P

-

L

-

V

-

I

-

P

-

D

R

K

L

I

L

M

M

M

G

G

P

P

G

G

P

P

S

S

N

N

V

C

S

S

P

K

E

R

V

V

L

L

A

A

Q

L

A

N

R

N

P

T

T

C

I

L

G

S

H

N

L

F

D

H

P

D

S

E

F

L

V

F

G

Q

L

V

M

I

D

D

R

E

I

V

K

K

D

D

Q

G

L

L

R

R

L

Y

A

I

F

F

R

Q

T

T

D

E

N

N

R

R

V

T

T

T

F

M

P

C

L

I

S

T

A

G

P
x
S

A
|
A

S
x
H

L

T

A

G

M

M

E

E

M

A

A

L

L

L

V

C

T

N

L

L

E

E

P

E

G

G

D

D

T

I

A

V

I

L

I

I

A

A

Q

N

N

N

G

G

V

I

F
x
W

G

A

G

E

R

R

M

A

A

I

E

N

I

M

A

A

Q

T

R

R

A

Y

G

G

A

A

E

D

V

V

R

R

L

V

V

L

S

E

V

G

E

P

W

A

G

D

K

K

P

P

V

F

D

S

P

M

E

T

A

D

V

F

R

K

A

K

S

A

I

I

L

-

E

E

A

Q

P

H

Q

R

A

P

K

K

L

C

L

L

A

F

F

V

V

V

H

H

A

G

E

D

T
x
S

S

S

T

S

G

G

V

L

R

L

S

Q

D

P

A

L

A

E

S

G

L

L

C

G

A

K

L

I

A

C

R

H

E

D

A

Y

G

D

L

C

L

L

S

L

V

L

V

V

D
|
D

T

A

V
|
V

T

A

G

S

L

L

G

C

G

G

I

V

P

P

V

F

S

Y

V

M

D

D

E

K

W

W

Q

E

A

I

D

D

A

G

V

V

Y

Y

A

T

G

G

T

S

Q
|
Q

K
|
K

C

V

L

L

S

G

A

A

P

P

G

G

L

I

A

T

P

P

I

I

T

S

F

I

S

S

D

P

R

K

A

A

V

L

S

E

A

V

V

I

K

R

A

S

R

R

K

K

T

T

P

P

I

S

Q

K

S

V

W

F

F

Y

L

W

D

D

L

L

G

L

L

I

M

L

L

G

G

N

Y

Y

W

W

E

G

G

C

-

Y

E

D

G

E

A

Q

R

K

S

R

Y
|
Y

H

H

T
|
T

A

V

P

P

V

S

N

N

A

L

L

I

Y

F

G

A

L

L

H

R

E

E

S

A

L

I

S

A

R

Q

L

I

L

A

G

E

E

E

G

G

L

L

E

E

T

P

A

V

W

I

A

R

R

R

H

R

R

Q

A

E

A

C

H

A

D

E

R

Q

L

M

V

Y

E

R

R

G

L

L

Q

Q

G

A

L

M

G

G

I

L

A

E

-

I

F

F

V

V

V

K

D

D

K

P

E

E

H

Y

R

R

L

L

P

P

Q

T

L

V

N

T

T

C

V

I

W

M

L

I

P

P

E

K

G

G

V

V

K

N

D

W

V

W

P

K

E

V

R

S

R

E

R

Y

L

A

L

M

D

N

E

N

F

F

G

S

I

L

E

E

I

I

G

Q

G

G

L

F

G

G

P

P

L

T

A

M

G

G

R

I

I

A

W

W

R

R

I

A

G

G

L

I

M

M

G

G

E

E

T

S

C

S

R

T

I

L

E

Q

N

R

V

V

D

N

R

F

L

Y

A

L

E

Y

A

A

I

F

A

K

A

E

V

S

L

L

binding pyridoxal-5'-phosphate: S77 (≠ P67), A78 (= A68), H79 (≠ S69), W104 (≠ F94), S154 (≠ T145), D179 (= D170), V181 (= V172), Q204 (= Q195), K205 (= K196), Y256 (= Y246), T259 (= T249)

1h0cA The crystal structure of human alanine:glyoxylate aminotransferase (see paper)
39% identity, 100% coverage: 1:369/370 of query aligns to 12:379/385 of 1h0cA

query
sites
1h0cA

M

L

N

K

A

P

F

L

S

S

P

I

P

P

P

N

R

Q

L

L

M

L

G

G

P
|
P

G
|
G

P

P

S

S

N

N

V

L

S

P

P

P

E

R

V

I

L

M

A

A

Q

G

A

G

R

L

P

Q

T

M

I

I

G

G

H

S

L

M

D

S

P

K

S

D

F

M

V

Y

G

Q

L

I

M

M

D

D

R

E

I

I

K

K

D

E

Q

G

L

I

R

Q

L

Y

A

V

F

F

R

Q

T

T

D

R

N

N

R

P

V

L

T

T

F

L

P

V

L

I

S

S

A

G

P
x
S

A
x
G

S
x
H

L

C

A

A

M

L

E

E

M

A

A

A

L

L

V

V

T

N

L

V

L

L

E

E

P

P

G

G

D

D

T

S

A

F

I

L

I

V

A

G

Q

A

N

N

G

G

V

I

F
x
W

G

G

G

Q

R

R

M

A

A

V

E

D

I

I

A

G

Q

E

R

R

A

I

G

G

A

-

E

-

V

V

R

H

L

P

V

M

S

T

V

K

E

D

W

P

G

G

K

G

P

H

V

Y

D

T

P

L

E

Q

A

E

V

V

R

E

A

E

S

G

I

L

L

A

E

Q

A

H

P

-

Q

K

A

P

K

V

L

L

A

F

F

L

V

T

H

H

A

G

E

E

T
x
S

S

S

T

T

G

G

V

V

R

L

S

Q

D

P

A

L

A

D

S

G

L

F

C

G

A

E

L

L

A

C

R

H

E

R

A

Y

G

K

L

C

L

L

S

L

V

L

V

V

D
|
D

T

S

V
|
V

T

A

G

S

L

L

G

G

I

T

P

P

V

L

S

Y

V

M

D

D

E

R

W

Q

Q

G

A

I

D

D

A

I

V

L

Y

Y

A

S

G

G

T

S

Q

Q

K
|
K

C

A

L

L

S

N

A

A

P

P

G

G

L

T

A

S

P

L

I

I

T

S

F

F

S

S

D

D

R

K

A

A

V

K

S

K

A

K

V

M

K

Y

A

S

R

R

K

K

T

T

P

K

I

P

Q

F

S

S

W

F

F

Y

L

L

D

D

L

I

G

K

L

W

M

L

L

A

G

N

Y

F

W

W

E

G

G

C

E

D

G

D

A

Q

-

P

R

R

S

M

Y

Y

H

H

T

T

A

I

P

P

V

V

N

I

A

S

L

L

Y

Y

G

S

L

L

H

R

E

E

S

S

L

L

S

A

R

L

L

I

L

A

G

E

E

Q

G

G

L

L

E

E

T

N

A

S

W

W

A

R

R

Q

H

H

R

R

A

E

A

A

H

A

D

A

R

Y

L

L

V

H

E

G

R

R

L

L

Q

Q

G

A

L

L

G

G

I

L

A

Q

-

L

F

F

V

V

V

K

D

D

K

P

E

A

H

L

R

R

L

L

P

P

Q

T

L

V

N

T

T

T

V

V

W

A

L

V

P

P

E

A

G

G

V

Y

K

D

D

W

V

R

P

D

E

I

R

V

R

S

R

Y

L

V

L

I

D

D

E

H

F

F

G

D

I

I

E

E

I

I

G

M

G

G

L
|
L

G

G

P

P

L

S

A

T

G

G

R

K

I

V

W

L

R
|
R

I

I

G

G

L

L

M

L

G

G

E

C

T

N

C

A

R

T

I

R

E

E

N

N

V

V

D

D

R

R

L

V

A

T

E

E

A

A

I

L

A

R

A

A

V

A

L

L

binding (aminooxy)acetic acid: P25 (= P14), G26 (= G15), L346 (= L336), R355 (= R345)
binding pyridoxal-5'-phosphate: S78 (≠ P67), G79 (≠ A68), H80 (≠ S69), W105 (≠ F94), S153 (≠ T145), D178 (= D170), V180 (= V172), K204 (= K196)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 385

2ch1A Structure of anopheles gambiae 3-hydroxykynurenine transaminase (see paper)
35% identity, 96% coverage: 4:358/370 of query aligns to 2:368/388 of 2ch1A

query
sites
2ch1A

F

F

S

T

P

P

-

A

-

S

-

L

-

R

-

N

-

P

-

L

-

I

-

P

-

E

R

K

L

I

L

M

M

M

G

G

P

P

G

G

P

P

S

S

N

N

V

C

S

S

P

K

E

R

V

V

L

L

A

T

A

A

Q

M

A

T

R

N

P

T

T

V

I

L

G

S

H

N

L

F

D

H

P

A

S

E

F

L

V

F

G

R

L

T

M

M

D

D

R

E

I

V

K

K

D

D

Q

G

L

L

R

R

L

Y

A

I

F

F

R

Q

T

T

D

E

N

N

R

R

V

A

T

T

F

M

P

C

L

V

S

S

A

G

P
x
S

A
|
A

S
x
H

L

A

A

G

M

M

E

E

M

A

A

M

L

L

V

S

T

N

L

L

E

E

P

E

G

G

D

D

T

R

A

V

I

L

I

I

A

A

Q

V

N

N

G

G

V

I

F
x
W

G

A

G

E

R

R

M

A

A

V

E

E

I

M

A

S

Q

E

R

R

A

Y

G

G

A

A

E

D

V

V

R

R

L

T

V

I

S

E

V

G

E

P

W

P

G

D

K

R

P

P

V

F

D

S

P

L

E

E

A

T

V

L

R

-

A

A

S

R

I

A

L

I

E

E

A

L

P

H

Q

Q

A

P

K

K

L

C

L

L

A

F

F

L

V

T

H

H

A

G

E

D

T
x
S

S

S

T

S

G

G

V

L

R

L

S

Q

D

P

A

L

A

E

S

G

L

V

C

G

A

Q

L

I

A

C

R

H

E

Q

A

H

G

D

L

C

L

L

S

L

V

I

V

V

D
|
D

T

A

V
|
V

T

A

G

S

L

L

G

C

G

G

I

V

P

P

V

F

S

Y

V

M

D

D

E

K

W

W

Q

E

A

I

D

D

A

A

V

V

Y

Y

A

T

G

G

T

A

Q
|
Q

K
|
K

C

V

L

L

S

G

A

A

P

P

G

G

L

I

A

T

P

P

I

I

T

S

F

I

S

S

D

P

R

K

A

A

V

L

S

D

A

V

V

I

K

R

A

N

R

R

K

R

T

T

P

K

I

S

Q

K

S

V

W

F

F

Y

L

W

D

D

L

L

G

L

L

L

M

L

L

G

G

N

Y

Y

W

W

E

G

G

C

-

Y

E

D

G

E

A

P

R

K

S

R

Y
|
Y

H

H

T
|
T

A

V

P

A

V

S

N

N

A

L

L

I

Y

F

G

A

L

L

H

R

E

E

S

A

L

L

S

A

R

Q

L

I

L

A

G

E

E

E

G

G

L

L

E

E

T

N

A

Q

W

I

A

K

R

R

H

R

R

I

A

E

A

C

H

A

D

Q

R

I

L

L

V

Y

E

E

R

G

L

L

Q

G

G

K

L

M

G

G

I

L

-

D

A

I

F

F

V

V

V

K

D

D

K

P

E

R

H

H

R

R

L

L

P

P

Q

T

L

V

N

T

T

G

V

I

W

M

L

I

P

P

E

K

G

G

V

V

K

D

D

W

V

W

P

K

E

V

R

S

R

Q

R

Y

L

A

L

M

D

N

E

N

F

F

G

S

I

L

E

E

I

V

G

Q

G

G

L

L

G

G

P

P

L

T

A

F

G

G

R

K

I

A

W

W

R

R

I

V

G

G

L

I

M

M

G

G

E

E

T

C

C

S

R

T

I

V

E

Q

N

K

V

I

binding pyridoxal-5'-phosphate: S76 (≠ P67), A77 (= A68), H78 (≠ S69), W103 (≠ F94), S153 (≠ T145), D178 (= D170), V180 (= V172), Q203 (= Q195), K204 (= K196), Y255 (= Y246), T258 (= T249)

Q7PRG3 3-hydroxykynurenine transaminase; AgHKT; Alanine--glyoxylate aminotransferase; EC 2.6.1.63; EC 2.6.1.44 from Anopheles gambiae (African malaria mosquito) (see paper)
35% identity, 96% coverage: 4:358/370 of query aligns to 3:369/396 of Q7PRG3

query
sites
Q7PRG3

F

F

S

T

P

P

-

A

-

S

-

L

-

R

-

N

-

P

-

L

-

I

-

P

-

E

R

K

L

I

L

M

M

M

G

G

P

P

G

G

P

P

S

S

N

N

V

C

S

S

P

K

E

R

V

V

L

L

A

T

A

A

Q

M

A

T

R

N

P

T

T

V

I

L

G

S

H

N

L

F

D

H

P

A

S

E

F

L

V

F

G

R

L

T

M

M

D

D

R

E

I

V

K

K

D

D

Q

G

L

L

R

R

L

Y

A

I

F

F

R

Q

T

T

D

E

N

N

R

R

V

A

T

T

F

M

P

C

L

V

S

S

A

G

P
x
S

A
|
A

S
x
H

L

A

A

G

M

M

E

E

M

A

A

M

L

L

V

S

T

N

L

L

E

E

P

E

G

G

D

D

T

R

A

V

I

L

I

I

A

A

Q

V

N

N

G

G

V

I

F

W

G

A

G

E

R

R

M

A

A

V

E

E

I

M

A

S

Q

E

R

R

A

Y

G

G

A

A

E

D

V

V

R

R

L

T

V

I

S

E

V

G

E

P

W

P

G

D

K

R

P

P

V

F

D

S

P

L

E

E

A

T

V

L

R

-

A

A

S

R

I

A

L

I

E

E

A

L

P

H

Q

Q

A

P

K

K

L

C

L

L

A

F

F

L

V

T

H

H

A

G

E

D

T
x
S

S

S

T

S

G

G

V

L

R

L

S

Q

D

P

A

L

A

E

S

G

L

V

C

G

A

Q

L

I

A

C

R

H

E

Q

A

H

G

D

L

C

L

L

S

L

V

I

V

V

D

D

T

A

V

V

T

A

G

S

L

L

G

C

G

G

I

V

P

P

V

F

S

Y

V

M

D

D

E

K

W

W

Q

E

A

I

D

D

A

A

V

V

Y

Y

A

T

G

G

T

A

Q
|
Q

K
|
K

C

V

L

L

S

G

A

A

P

P

G

G

L

I

A

T

P

P

I

I

T

S

F

I

S

S

D

P

R

K

A

A

V

L

S

D

A

V

V

I

K

R

A

N

R

R

K

R

T

T

P

K

I

S

Q

K

S

V

W

F

F

Y

L

W

D

D

L

L

G

L

L

L

M

L

L

G

G

N

Y

Y

W

W

E

G

G

C

-

Y

E

D

G

E

A

P

R

K

S

R

Y
|
Y

H

H

T
|
T

A

V

P

A

V

S

N

N

A

L

L

I

Y

F

G

A

L

L

H

R

E

E

S

A

L

L

S

A

R

Q

L

I

L

A

G

E

E

E

G

G

L

L

E

E

T

N

A

Q

W

I

A

K

R

R

H

R

R

I

A

E

A

C

H

A

D

Q

R

I

L

L

V

Y

E

E

R

G

L

L

Q

G

G

K

L

M

G

G

I

L

-

D

A

I

F

F

V

V

V

K

D

D

K

P

E

R

H

H

R

R

L

L

P

P

Q

T

L

V

N

T

T

G

V

I

W

M

L

I

P

P

E

K

G

G

V

V

K

D

D

W

V

W

P

K

E

V

R

S

R

Q

R

Y

L

A

L

M

D

N

E

N

F

F

G

S

I

L

E

E

I

V

G

Q

G

G

L

L

G

G

P

P

L

T

A

F

G

G

R

K

I

A

W

W

R

R

I

V

G

G

L

I

M

M

G

G

E

E

T

C

C

S

R

T

I

V

E

Q

N

K

V

I

SAH 77:79 (≠ PAS 67:69) binding in other chain
S154 (≠ T145) binding in other chain
Q204 (= Q195) binding in other chain
K205 (= K196) modified: N6-(pyridoxal phosphate)lysine
Y256 (= Y246) binding
T259 (= T249) binding

2ch2A Structure of the anopheles gambiae 3-hydroxykynurenine transaminase in complex with inhibitor (see paper)
35% identity, 96% coverage: 4:358/370 of query aligns to 1:367/387 of 2ch2A

query
sites
2ch2A

F

F

S

T

P

P

-

A

-

S

-

L

-

R

-

N

-

P

-

L

-

I

-

P

-

E

R

K

L

I

L

M

M

M

G

G

P

P

G
|
G

P

P

S

S

N

N

V

C

S

S

P

K

E

R

V

V

L

L

A

T

A

A

Q

M

A

T

R

N

P

T

T

V

I

L

G
x
S

H
x
N

L

F

D

H

P

A

S

E

F

L

V

F

G

R

L

T

M

M

D

D

R

E

I

V

K

K

D

D

Q

G

L

L

R

R

L

Y

A

I

F

F

R

Q

T

T

D

E

N

N

R

R

V

A

T

T

F

M

P

C

L

V

S

S

A

G

P
x
S

A
|
A

S
x
H

L

A

A

G

M

M

E

E

M

A

A

M

L

L

V

S

T

N

L

L

E

E

P

E

G

G

D

D

T

R

A

V

I

L

I

I

A

A

Q

V

N

N

G

G

V

I

F
x
W

G

A

G

E

R

R

M

A

A

V

E

E

I

M

A

S

Q

E

R

R

A

Y

G

G

A

A

E

D

V

V

R

R

L

T

V

I

S

E

V

G

E

P

W

P

G

D

K

R

P

P

V

F

D

S

P

L

E

E

A

T

V

L

R

-

A

A

S

R

I

A

L

I

E

E

A

L

P

H

Q

Q

A

P

K

K

L

C

L

L

A

F

F

L

V

T

H

H

A

G

E

D

T
x
S

S

S

T

S

G

G

V

L

R

L

S

Q

D

P

A

L

A

E

S

G

L

V

C

G

A

Q

L

I

A

C

R

H

E

Q

A

H

G

D

L

C

L

L

S

L

V

I

V

V

D
|
D

T

A

V
|
V

T

A

G

S

L

L

G

C

G

G

I

V

P

P

V

F

S

Y

V

M

D

D

E

K

W

W

Q

E

A

I

D

D

A

A

V

V

Y

Y

A

T

G

G

T

A

Q

Q

K
|
K

C

V

L

L

S

G

A

A

P

P

G

G

L

I

A

T

P

P

I

I

T

S

F

I

S

S

D

P

R

K

A

A

V

L

S

D

A

V

V

I

K

R

A

N

R

R

K

R

T

T

P

K

I

S

Q

K

S

V

W

F

F

Y

L

W

D

D

L

L

G

L

L

L

M

L

L

G

G

N

Y

Y

W

W

E

G

G

C

-

Y

E

D

G

E

A

P

R

K

S

R

Y
|
Y

H

H

T
|
T

A

V

P

A

V

S

N

N

A

L

L

I

Y

F

G

A

L

L

H

R

E

E

S

A

L

L

S

A

R

Q

L

I

L

A

G

E

E

E

G

G

L

L

E

E

T

N

A

Q

W

I

A

K

R

R

H

R

R

I

A

E

A

C

H

A

D

Q

R

I

L

L

V

Y

E

E

R

G

L

L

Q

G

G

K

L

M

G

G

I

L

-

D

A

I

F

F

V

V

V

K

D

D

K

P

E

R

H

H

R

R

L

L

P

P

Q

T

L

V

N

T

T

G

V

I

W

M

L

I

P

P

E

K

G

G

V

V

K

D

D

W

V

W

P

K

E

V

R

S

R

Q

R

Y

L

A

L

M

D

N

E

N

F

F

G

S

I

L

E

E

I

V

G
x
Q

G

G

L
|
L

G

G

P

P

L

T

A

F

G

G

R

K

I

A

W

W

R
|
R

I

V

G

G

L

I

M

M

G

G

E

E

T

C

C

S

R

T

I

V

E

Q

N

K

V

I

binding 4-(2-aminophenyl)-4-oxobutanoic acid: G23 (= G15), S41 (≠ G33), N42 (≠ H34), S152 (≠ T145), Y254 (= Y246), Q342 (≠ G333), L345 (= L336), R354 (= R345)
binding pyridoxal-5'-phosphate: S75 (≠ P67), A76 (= A68), H77 (≠ S69), W102 (≠ F94), S152 (≠ T145), D177 (= D170), V179 (= V172), K203 (= K196), Y254 (= Y246), T257 (= T249)

2yrrA Hypothetical alanine aminotransferase (tth0173) from thermus thermophilus hb8
38% identity, 96% coverage: 10:365/370 of query aligns to 2:346/352 of 2yrrA

query
sites
2yrrA

L

L

M

L

G

T

P

P

G

G

P

P

S

T

N

P

V

I

S

P

P

E

E

R

V

V

L

Q

A

K

A

A

Q

L

A

L

R

R

P

P

T

M

I

R

G

G

H

H

L

L

D

D

P

P

S

E

F

V

V

L

G

R

L

V

M

N

D

R

R

A

I

I

K

Q

D

E

Q

R

L

L

R

A

L

A

A

L

F

F

R

D

T

P

-

G

D

E

N

G

R

A

V

L

T

V

F

A

P

A

L

L

S

A

A

G

P
x
S

A
x
G

S
|
S

L

L

A

G

M

M

E

E

M

A

A

G

L

L

V

A

T

N

L

L

L

-

E

D

P

R

G

G

D

P

T

V

A

L

I

V

I

L

A

V

Q

-

N

N

G

G

V

A

F
|
F

G

S

G

Q

R

R

M

V

A

A

E

E

I

M

A

A

Q

A

R

L

A

H

G

G

A

L

E

D

V

P

R

E

L

V

V

L

S

D

V

F

E

P

W

P

G

G

K

E

P

P

V

V

D

D

P

P

E

E

A

A

V

V

R

-

A

A

S

R

I

A

L

L

E

K

A

R

P

R

Q

R

A

Y

K

R

L

M

L

V

A

A

F

L

V

V

H

H

A

G

E

E

T
|
T

S

S

T

T

G

G

V

V

R

L

S

N

D

P

A

A

A

E

S

A

L

I

C

G

A

A

L

L

A

A

R

K

E

E

A

A

G

G

L

A

L

L

S

F

V

F

V

L

D
|
D

T

A

V
|
V

T

T

G

T

L

L

G

G

G

M

I

L

P

P

V

F

S

S

V

M

D

R

E

A

W

M

Q

G

A

V

D

D

A

Y

V

A

Y

F

A

T

G

G

T

S

Q

Q

K
|
K

C

C

L

L

S

S

A

A

P

P

G

G

L

L

A

A

P

P

I

I

T

A

F

A

S

S

D

L

R

E

A

A

V

R

S

K

A

A

V

F

K

T

A

G

R

K

K

R

T

-

P

-

I

-

Q

-

S

G

W

W

F

Y

L

L

D

D

L

L

G

A

L

R

M

V

L

A

G

E

Y

H

W

W

E

E

G

R

E

G

G

G

A

-

R

-

S

-

Y

Y

H

H

T

T

A

T

P

P

V

V

N

L

A

L

L

H

Y

Y

G

A

L

L

H

L

E

E

S

A

L

L

S

D

R

L

L

V

L

L

G

E

E

E

G

G

L

V

E

A

T

A

A

R

W

E

A

R

R

R

H

A

R

R

A

E

A

V

H

Y

D

A

R

W

L

V

V

L

E

E

R

E

L

L

Q

K

G

A

L

R

G

G

I

F

A

R

F

-

V

P

V

Y

D

P

K

K

E

A

H

S

R

P

L

L

P

P

Q

T

L

V

N

L

T

V

V

V

W

R

L

P

P

P

E

E

G

G

V

V

K

-

D

D

V

A

P

D

E

R

R

L

R

V

R

R

L

A

L

L

D

Y

E

A

F

E

G

G

I

V

E

A

I

V

G

A

G

G

L

I

G

G

P

P

L

T

A

R

G

G

R

Q

I

V

W

L

R

R

I

L

G

G

L

L

M

M

G

G

E

E

T

G

C

A

R

R

I

R

E

E

N

A

V

Y

D

Q

R

A

L

F

A

L

E

K

A

A

I

L

binding pyridoxal-5'-phosphate: S60 (≠ P67), G61 (≠ A68), S62 (= S69), F85 (= F94), T135 (= T145), D160 (= D170), V162 (= V172), K186 (= K196)

Query Sequence

>SMa1495 SMa1495 aminotransferase
MNAFSPPPRLLMGPGPSNVSPEVLAAQARPTIGHLDPSFVGLMDRIKDQLRLAFRTDNRV
TFPLSAPASLAMEMALVTLLEPGDTAIIAQNGVFGGRMAEIAQRAGAEVRLVSVEWGKPV
DPEAVRASILEAPQAKLLAFVHAETSTGVRSDAASLCALAREAGLLSVVDTVTGLGGIPV
SVDEWQADAVYAGTQKCLSAPPGLAPITFSDRAVSAVKARKTPIQSWFLDLGLMLGYWEG
EGARSYHHTAPVNALYGLHESLSRLLGEGLETAWARHRAAHDRLVERLQGLGIAFVVDKE
HRLPQLNTVWLPEGVKDVPERRRLLDEFGIEIGGGLGPLAGRIWRIGLMGETCRIENVDR
LAEAIAAVLP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory