SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMa1871 FitnessBrowser__Smeli:SMa1871 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1omoA Alanine dehydrogenase dimer w/bound NAD (archaeal) (see paper)
38% identity, 100% coverage: 1:329/330 of query aligns to 1:320/320 of 1omoA

query
sites
1omoA

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active site: R52 (= R50), D219 (= D228)
binding nicotinamide-adenine-dinucleotide: R108 (= R114), T109 (= T115), G132 (= G138), C133 (≠ T139), G134 (= G140), T135 (≠ N141), Q136 (= Q142), Y156 (≠ W162), D157 (≠ S163), V158 (≠ R164), R159 (≠ T165), T194 (= T203), T195 (= T204), P196 (≠ R205), V201 (≠ L210), I216 (= I225), G217 (= G226), D219 (= D228), K223 (= K232), S290 (= S299), T291 (= T300), G292 (= G301)

O28608 Alanine dehydrogenase; AlaDH; EC 1.4.1.1 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
38% identity, 100% coverage: 1:329/330 of query aligns to 1:320/322 of O28608

query
sites
O28608

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R108 (= R114) binding
TQ 135:136 (≠ NQ 141:142) binding
DVR 157:159 (≠ SRT 163:165) binding
GAD 217:219 (≠ GTD 226:228) binding
K223 (= K232) binding
S290 (= S299) binding

6t3eB Structure of thermococcus litoralis delta(1)-pyrroline-2-carboxylate reductase in complex with nadh and l-proline (see paper)
35% identity, 98% coverage: 4:328/330 of query aligns to 2:325/325 of 6t3eB

query
sites
6t3eB

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binding 1,4-dihydronicotinamide adenine dinucleotide: L71 (≠ G73), S82 (≠ N86), V83 (≠ S87), T107 (= T111), R110 (= R114), T111 (= T115), G134 (= G138), A135 (≠ T139), G136 (= G140), V137 (≠ N141), Q138 (= Q142), Y158 (≠ W162), D159 (≠ S163), I160 (≠ R164), N164 (≠ S168), A199 (≠ T203), T200 (= T204), T201 (≠ R205), A202 (≠ G206), V206 (≠ L210), V221 (≠ I225), G222 (= G226), W223 (≠ T227), S296 (= S299), V297 (≠ T300), G298 (= G301)
binding proline: R39 (≠ Y41), L52 (≠ D54), M54 (≠ K56), K67 (= K69), V69 (≠ H71), V83 (≠ S87), R110 (= R114), W223 (≠ T227), V297 (≠ T300)

6rqaB Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
38% identity, 83% coverage: 45:317/330 of query aligns to 48:311/322 of 6rqaB

query
sites
6rqaB

H
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W

H

V

V

K

K

P

G

G

P

T

T

H

H

I

I

V

A

A

A

I
x
M

G
|
G

T

T

D
|
D

Q

T

R

K

G

G

K
|
K

Q

Q

E

E

L

L

D

D

P

P

E

A

I

L

F

V

R

A

N

R

A

A

K

R

V

I

V

F

V

T

D

D

S

E

V

V

A

A

Q

Q

C

S

T

V

E

S

K

I

G

G

E

E

T

C

W

Q

H

H

P

A

L

I

N

A

K

A

N

G

I

L

I

I

T

R

K

E

D

D

D

Q

I

V

H

-

G

G

E

E

I

L

G

G

E

A

V

V

L

V

L

A

G

G

R

D

K

D

P

P

G

G

R

R

E

-

S

G

D

D

D

A

E

E

I

V

T

T

I

I

F

F

D

D

S
x
G

T
|
T

G
|
G

M

V

A

G

I

L

Q

Q

D

D

N

L

T

A

T

V

A

A

S

Q

K

A

I

V

Y

V

Q

E

N

L

A

A

binding Tb-Xo4: H48 (= H45), E49 (≠ H46), D50 (≠ P47), N76 (= N78)
binding nicotinamide-adenine-dinucleotide: R112 (= R114), T113 (= T115), G136 (= G138), G138 (= G140), H139 (≠ N141), Q140 (= Q142), N161 (≠ S163), P162 (≠ R164), H163 (≠ T165), I199 (≠ T203), T200 (= T204), S201 (≠ R205), S202 (≠ G206), M221 (≠ I225), G222 (= G226), D224 (= D228), K228 (= K232), G293 (≠ S299), T294 (= T300), G295 (= G301)

6rqaA Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
38% identity, 83% coverage: 45:317/330 of query aligns to 48:311/322 of 6rqaA

query
sites
6rqaA

H

H

H

E

P

D

S

A

L

L

R

Y

G

G

E

-

I

-

D

-

F

F

K

K

L

G

G

G

Y

F

Y

D

K

A

A

S

N

A

E

L

I

V

I

L

S

G

M

L

K

K

A

A

H

-

S

-

G

G

G

G

F

Y

T

W

N

P

N

N

P

N

A

Q

E

K

H

H

G

N

V

L

P

I

N

N

S
x
H

I

Q

G

S

T

T

I

V

L

F

L

L

F

F

D

D

A

P

R

D

S

T

C

G

A

R

L

V

I

S

C

A

I

A

M

V

D

G

G

G

S

N

L

L

I

L

T

T

G

A

L

L

R

R

T
|
T

G

A

A

A

S

A

G

S

A

A

V

V

S

S

V

I

K

K

A

Y

L

L

A

A

R

P

K

K

N

G

A

A

R

K

T

V

V

L

A

G

S

M

I

I

G

G

T

A

G
|
G

N
x
H

Q
|
Q

A

S

R

A

M

F

Q

Q

I

M

R

R

A

A

V

A

N

A

E

N

I

V

M

H

K

R

I

F

E

E

K

K

I

V

H

I

A

G

W

W

S
x
N

R
x
P

T
x
H

P

P

E

E

S
x
M

L

L

S

S

R

R

Y

L

K

-

T

A

D

D

I

T

Q

A

R

A

E

E

F

L

G

G

I

L

P

P

V

F

I

-

V

-

A

-

S

-

S

-

K

-

K

-

E

E

A

A

V

V

E

E

-

L

-

D

-

R

-

L

-

G

-

A

Q

E

A

A

D

D

I

V

L

I

I

V

T

S

T
x
I

T
|
T

R
x
S

G
x
S

K

F

G

S

S

P

L

L

V

L

E

M

A

N

D

E

W

H

V

V

K

K

P

G

G

P

T

T

H

H

I

I

V

A

A

A

I
x
M

G
|
G

T

T

D
|
D

Q

T

R

K

G

G

K
|
K

Q

Q

E

E

L

L

D

D

P

P

E

A

I

L

F

V

R

A

N

R

A

A

K

R

V

I

V

F

V

T

D

D

S

E

V

V

A

A

Q

Q

C

S

T

V

E

S

K

I

G

G

E

E

T

C

W

Q

H

H

P

A

L

I

N

A

K

A

N

G

I

L

I

I

T

R

K

E

D

D

D

Q

I

V

H

-

G

G

E

E

I

L

G

G

E

A

V

V

L

V

L

A

G

G

R

D

K

D

P

P

G

G

R

R

E

-

S

G

D

D

D

A

E

E

I

V

T

T

I

I

F

F

D

D

S
x
G

T
|
T

G
|
G

M

V

A

G

I

L

Q

Q

D

D

N

L

T

A

T

V

A

A

S

Q

K

A

I

V

Y

V

Q

E

N

L

A

A

binding nicotinamide-adenine-dinucleotide: H85 (≠ S87), T113 (= T115), G138 (= G140), H139 (≠ N141), Q140 (= Q142), N161 (≠ S163), P162 (≠ R164), H163 (≠ T165), M166 (≠ S168), I199 (≠ T203), T200 (= T204), S201 (≠ R205), S202 (≠ G206), M221 (≠ I225), G222 (= G226), D224 (= D228), K228 (= K232), G293 (≠ S299), T294 (= T300), G295 (= G301)

A1B8Z0 Iminosuccinate reductase; EC 1.4.1.- from Paracoccus denitrificans (strain Pd 1222) (see paper)
38% identity, 83% coverage: 45:317/330 of query aligns to 46:309/320 of A1B8Z0

query
sites
A1B8Z0

H

H

H

E

P

D

S

A

L

L

R

Y

G

G

E

-

I

-

D

-

F

F

K

K

L

G

G

G

Y

F

Y

D

K

A

A

S

N

A

E

L

I

V

I

L

S

G

M

L

K

K

A

A

H

-

S

-

G

G

G

G

F

Y

T

W

N

P

N

N

P

N

A

Q

E

K

H

H

G

N

V

L

P

I

N

N

S

H

I

Q

G

S

T

T

I

V

L

F

L

L

F

F

D

D

A

P

R

D

S

T

C

G

A

R

L

V

I

S

C

A

I

A

M

V

D

G

G

G

S

N

L

L

I

L

T

T

G

A

L

L

R
|
R

T

T

G

A

A

A

S

A

G

S

A

A

V

V

S

S

V

I

K

K

A

Y

L

L

A

A

R

P

K

K

N

G

A

A

R

K

T

V

V

L

A

G

S

M

I

I

G

G

T

A

G

G

N
x
H

Q
|
Q

A

S

R

A

M

F

Q

Q

I

M

R

R

A

A

V

A

N

A

E

N

I

V

M

H

K

R

I

F

E

E

K

K

I

V

H

I

A

G

W

W

S
x
N

R

P

T

H

P

P

E

E

S

M

L

L

S

S

R

R

Y

L

K

-

T

A

D

D

I

T

Q

A

R

A

E

E

F

L

G

G

I

L

P

P

V

F

I

-

V

-

A

-

S

-

S

-

K

-

K

-

E

E

A

A

V

V

E

E

-

L

-

D

-

R

-

L

-

G

-

A

Q

E

A

A

D

D

I

V

L

I

I

V

T

S

T

I

T

T

R
x
S

G

S

K

F

G

S

S

P

L

L

V

L

E

M

A

N

D

E

W

H

V

V

K

K

P

G

G

P

T

T

H

H

I

I

V

A

A

A

I
x
M

G
|
G

T
|
T

D
|
D

Q

T

R

K

G

G

K
|
K

Q

Q

E

E

L

L

D

D

P

P

E

A

I

L

F

V

R

A

N

R

A

A

K

R

V

I

V

F

V

T

D

D

S

E

V

V

A

A

Q

Q

C

S

T

V

E

S

K

I

G

G

E

E

T

C

W

Q

H

H

P

A

L

I

N

A

K

A

N

G

I

L

I

I

T

R

K

E

D

D

D

Q

I

V

H

-

G

G

E

E

I

L

G

G

E

A

V

V

L

V

L

A

G

G

R

D

K

D

P

P

G

G

R

R

E

-

S

G

D

D

D

A

E

E

I

V

T

T

I

I

F

F

D

D

S
x
G

T

T

G

G

M

V

A

G

I

L

Q

Q

D

D

N

L

T

A

T

V

A

A

S

Q

K

A

I

V

Y

V

Q

E

N

L

A

A

R110 (= R114) binding
HQ 137:138 (≠ NQ 141:142) binding
N159 (≠ S163) binding
S199 (≠ R205) binding
MGTD 219:222 (≠ IGTD 225:228) binding
K226 (= K232) binding
G291 (≠ S299) binding

4mp6A Staphyloferrin b precursor biosynthetic enzyme sbnb bound to citrate and NAD+ (see paper)
30% identity, 94% coverage: 21:329/330 of query aligns to 23:334/334 of 4mp6A

query
sites
4mp6A

I

V

G

D

A

A

V

L

E

T

E

E

A

A

Y

L

K

T

A

A

F

H

S

A

S

H

D

N

Q

D

V

F

E

V

Q

Q

P

P

D

-

Y

-

I

-

G

-

I

-

H

L

H

K

P

P

S

Y

L

L

R

R

G

Q

E

-

I

-

D

D

F

P

K

E

L

N

G

G

Y

H

Y

I

K

-

A

A

N

D

E

R

I

I

I
|
I

S

A

M

M

K

P

A

S

H

H

S

I

G

G

G

G

-

E

-

H

-

A

-

I

-

S

-

G

-

I

-

K

-

W

-

I

-

G

-

S

F

K

T

H

N

D

N

N

P

P

A

S

E

K

H

R

G

N

V

M

P

E

N
x
R

S

A

I

S

G

G

T

V

I

I

L

I

L

L

F

N

D

D

A

P

R

E

S

T

C

N

A

Y

L

P

I

I

C

A

I

V

M

M

D

E

G

A

S

S

L

L

I

I

T

S

G

S

L

M

R
|
R

T
|
T

G

A

A

A

S

V

G

S

A

V

V

I

S

A

V

A

K

K

A

H

L

L

A

A

R

K

K

K

N

G

A

F

R

K

T

D

V

L

A

T

S

I

I

I

G
|
G

T
x
C

G
|
G

N
x
L

Q
x
I

A

G

R

D

M

K

Q

Q

I

L

R

Q

A

S

V

M

N

L

E

E

I

Q

M

F

-

D

K

H

I

I

E

E

K

R

I

V

H

F

A

V

W
x
Y

S
x
D

R
x
Q

T

F

P

S

E

E

S

A

L

C

S

A

R

R

Y

F

K

V

T

D

D

R

I

W

Q

Q

R

Q

E

Q

F

R

G

P

-

E

I

I

P

N

V

F

I

I

V

A

A

T

S

E

S

N

K

A

K

K

E

E

A

A

V

V

E

S

Q

N

A

G

D

E

I

V

L

V

I

I

T

T

T
x
C

T
|
T

R
x
V

G
x
T

K

D

G

Q

S

P

L
x
Y

V

I

E

E

A

Y

D

D

W

W

V

L

K

Q

P

K

G

G

T

A

H

F

I

I

V

S

A

N

I
|
I

G
x
S

T

I

D

-

Q

-

R

-

G

-

K

-

Q
x
M

E

D

L

V

D

H

P

K

E

E

I

V

F

F

R

I

N

K

A

A

-

D

K

K

V

V

V

V

V

V

D

D

S

D

V

W

A

S

Q

Q

C

C

T

N

E

R

K

E

G

K

E

K

T

T

W

I

H

N

P

Q

L

L

N

V

-

L

K

E

N

G

I

K

I

F

T

S

K

K

D

E

D

A

I

L

H

H

G

A

E

E

I

L

G

G

E

Q

V

L

L

V

L

T

G

G

R

D

K

I

P

P

G

G

R

R

E

E

S

D

D

D

D

D

E

E

I

I

T

I

I

L

F

L

D

N

S
x
P

T
x
M

G
|
G

M

M

A

A

I

I

Q

E

D

D

N

I

T

S

T

S

A

A

S

Y

K

F

I

I

Y

Y

Q

Q

N

Q

A

A

I

Q

A

Q

N

Q

N

N

V

I

G

G

T

T

F

T

F

L

Q

N

F

L

F

Y

active site: I60 (= I66), M236 (≠ Q233)
binding nicotinamide-adenine-dinucleotide: R92 (≠ N86), R120 (= R114), T121 (= T115), G144 (= G138), C145 (≠ T139), G146 (= G140), L147 (≠ N141), I148 (≠ Q142), Y169 (≠ W162), D170 (≠ S163), Q171 (≠ R164), C211 (≠ T203), T212 (= T204), V213 (≠ R205), T214 (≠ G206), Y218 (≠ L210), I233 (= I225), S234 (≠ G226), P304 (≠ S299), M305 (≠ T300), G306 (= G301)

4bv9A Crystal structure of the NADPH form of mouse mu-crystallin. (see paper)
33% identity, 71% coverage: 82:316/330 of query aligns to 78:300/303 of 4bv9A

query
sites
4bv9A

H

Y

G

E

V

V

P

P

N
x
S

S
x
H

I

Q

G

A

T

S

I

V

L

L

L

L

F

F

D

D

A

P

R

S

S

N

C

G

A

S

L

L

I

L

C

A

I

V

M

M

D

D

G

G

S

N

L

V

I

I

T
|
T

G

A

L

K

R
|
R

T
|
T

G

A

A

A

S

V

G

S

A

A

V

I

S

A

V

T

K

K

A

L

L

L

A

K

R

P

K

P

N

G

A

S

R

D

T

V

V

L

A

C

S

I

I

L

G
|
G

T
x
A

G
|
G

N
x
V

Q
|
Q

A

A

R

Y

M

S

Q

H

I

Y

R

E

A

I

V

F

N

T

E

E

I

Q

M

F

K

S

I

F

E

K

K

E

I

V

H

R

A

M

W

W

S
x
N

R
|
R

T
|
T

P

R

E

E

S
x
N

L

A

S

E

R

K

Y

F

K

A

T

S

D

T

I

V

Q

Q

R

G

E

D

F

-

G

-

I

-

P

-

V

V

I

R

V

V

A

C

S

S

S

S

K

V

K

Q

E

E

A

A

V

V

E

T

Q

G

A

A

D

D

I

V

L

I

I

I

T

T

T
x
V

T
|
T

R
x
M

G
x
A

K

T

G

E

S

P

L

I

V

L

E

F

A

G

D

E

W

W

V

V

K

K

P

P

G

G

T

A

H

H

I

I

V

N

A

A

I
x
V

G
|
G

T

A

D
x
S

Q

R

R

P

G

D

K

W

Q

R

E

E

L

L

D

D

P

D

E

E

I

L

F

M

R

R

N

Q

A

A

K

V

V

L

V

Y

V

V

D

D

S

S

V

R

-

E

A

A

Q

A

C

L

T

K

E

E

K

S

G

G

E

D

T

V

W

-

H

-

P

-

L

-

N

-

K

-

N

-

I

L

I

L

T

S

K

G

D

A

D

D

I

I

H

F

G

A

E

E

I

L

G

G

E

E

V

V

L

I

L

S

G

G

R

A

K

K

P

P

G

A

R

H

E

-

S

-

D

C

D

E

E

K

I

T

T

T

I

V

F

F

D

K

S
|
S

T
x
L

G
|
G

M

M

A

A

I

V

Q

E

D

D

N

L

T

V

T

A

A

A

S

K

K

L

I

V

Y

Y

Q

D

N

S

active site: S220 (≠ D228)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S82 (≠ N86), H83 (≠ S87), T107 (= T111), R110 (= R114), T111 (= T115), G134 (= G138), A135 (≠ T139), G136 (= G140), V137 (≠ N141), Q138 (= Q142), N159 (≠ S163), R160 (= R164), T161 (= T165), N164 (≠ S168), V195 (≠ T203), T196 (= T204), M197 (≠ R205), A198 (≠ G206), V217 (≠ I225), G218 (= G226), S220 (≠ D228), S283 (= S299), L284 (≠ T300), G285 (= G301)
binding pyruvic acid: T107 (= T111), R110 (= R114), L284 (≠ T300), G285 (= G301)

Sites not aligning to the query:

active site: 58
binding pyruvic acid: 45, 60, 73, 75

2i99A Crystal structure of human mu_crystallin at 2.6 angstrom (see paper)
31% identity, 90% coverage: 20:316/330 of query aligns to 22:308/312 of 2i99A

query
sites
2i99A

V

L

I

I

G

P

A

P

V

L

E

E

E

T

A

A

Y

L

K

A

A

N

F

F

S

S

S

S

D

G

Q

P

-

E

-

G

-

G

V

V

E

M

Q

Q

P

P

D

V

Y

R

I

T

G

V

I

V

H

P

H

V

P

T

S

K

L

H

R

R

G

G

E

Y

I

L

D
x
G

F

V

K

M

L

P

G

A

Y

Y

Y

S

K

A

A

A

N

E

E

D

I

A

I

L

S

T

M

T

K

K

A

L

H

V

S

T

G

F

G

-

F

Y

T

E

N
x
D

N

R

P

G

A

I

E

T

H

S

G

V

V

V

P

P

N
x
S

S
x
H

I

Q

G

A

T

T

I

V

L

L

L

L

F

F

D

E

A

P

R

S

S

N

C

G

A

T

L

L

I

L

C

A

I

V

M

M

D

D

G

G

S

N

L

V

I

I

T
|
T

G

A

L

K

R
|
R

T
|
T

G

A

A

A

S

V

G

S

A

A

V

I

S

A

V

T

K

K

A

F

L

L

A

K

R

P

K

P

N

S

A

S

R

E

T

V

V

L

A

C

S

I

I

L

G
|
G

T
x
A

G
|
G

N
x
V

Q
|
Q

A

A

R

Y

M

S

Q

H

I

Y

R

E

A

I

V

F

N

T

E

E

I

Q

M

F

K

S

I

F

E

K

K

E

I

V

H

R

A

I

W

W

S
x
N

R
|
R

T
|
T

P

K

E

E

S
x
N

L

A

S

E

R

K

Y

F

K

A

T

D

D

T

I

V

Q

Q

R

G

E

E

F

-

G

-

I

-

P

-

V

V

I

R

V

V

A

C

S

S

S

S

K

V

K

Q

E

E

A

A

V

V

E

A

Q

G

A

A

D

D

I

V

L

I

I

I

T

T

T
x
V

T
|
T

R
x
L

G
x
A

K

T

G

E

S

P

L

I

V

L

E

F

A

G

D

E

W

W

V

V

K

K

P

P

G

G

T

A

H

H

I

I

V

N

A

A

I
x
V

G
|
G

T

A

D
x
S

Q

R

R

P

G

D

K

W

Q

R

E

E

L

L

D

D

P

D

E

E

I

L

F

M

R

K

N

E

A

A

K

V

V

L

V

Y

V

V

D

D

S

S

V

Q

-

E

A

A

Q

A

C

L

T

K

E

E

K

S

G

G

E

D

T

V

W

-

H

-

P

-

L

-

N

-

K

-

N

-

I

L

I

L

T

S

K

G

D

A

D

E

I

I

H

F

G

A

E

E

I

L

G

G

E

E

V

V

L

I

L

K

G

G

R

V

K

K

P

P

G

A

R

H

E

-

S

-

D

C

D

E

E

K

I

T

T

T

I

V

F

F

D

K

S
|
S

T
x
L

G
|
G

M

M

A

A

I

V

Q

E

D

D

N

T

T

V

T

A

A

A

S

K

K

L

I

I

Y

Y

Q

D

N

S

active site: G59 (≠ D54), S228 (≠ D228)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: D81 (≠ N77), S90 (≠ N86), H91 (≠ S87), T115 (= T111), R118 (= R114), T119 (= T115), G142 (= G138), A143 (≠ T139), G144 (= G140), V145 (≠ N141), Q146 (= Q142), N167 (≠ S163), R168 (= R164), T169 (= T165), N172 (≠ S168), V203 (≠ T203), T204 (= T204), L205 (≠ R205), A206 (≠ G206), V225 (≠ I225), G226 (= G226), S228 (≠ D228), S291 (= S299), L292 (≠ T300), G293 (= G301)

Q14894 Ketimine reductase mu-crystallin; NADP-regulated thyroid-hormone-binding protein; EC 1.5.1.25 from Homo sapiens (Human) (see paper)
31% identity, 90% coverage: 20:316/330 of query aligns to 23:309/314 of Q14894

query
sites
Q14894

V

L

I

I

G

P

A

P

V

L

E

E

E

T

A

A

Y

L

K

A

A

N

F

F

S

S

S

S

D

G

Q

P

-

E

-

G

-

G

V

V

E

M

Q

Q

P

P

D

V

Y

R

I

T

G

V

I

V

H

P

H

V

P

T

S

K

L

H

R

R

G

G

E

Y

I

L

D

G

F

V

K

M

L

P

G

A

Y

Y

Y

S

K

A

A

A

N

E

E

D

I

A

I

L

S

T

M

T

K

K

A

L

H

V

S

T

G

F

G

-

F

Y

T

E

N

D

N

R

P

G

A

I

E

T

H

S

G

V

V

V

P

P

N

S

S

H

I

Q

G

A

T

T

I

V

L

L

L

L

F

F

D

E

A

P

R

S

S

N

C

G

A

T

L

L

I

L

C

A

I

V

M

M

D

D

G

G

S

N

L

V

I

I

T

T

G

A

L

K

R

R

T

T

G

A

A

A

S

V

G

S

A

A

V

I

S

A

V

T

K

K

A

F

L

L

A

K

R

P

K

P

N

S

A

S

R

E

T

V

V

L

A

C

S

I

I

L

G
|
G

T
x
A

G
|
G

N
x
V

Q
|
Q

A
|
A

R

Y

M

S

Q

H

I

Y

R

E

A

I

V

F

N

T

E

E

I

Q

M

F

K

S

I

F

E

K

K

E

I

V

H

R

A

I

W

W

S
x
N

R
|
R

T

T

P

K

E

E

S

N

L

A

S

E

R

K

Y

F

K

A

T

D

D

T

I

V

Q

Q

R

G

E

E

F

-

G

-

I

-

P

-

V

V

I

R

V

V

A

C

S

S

S

S

K

V

K

Q

E

E

A

A

V

V

E

A

Q

G

A

A

D

D

I

V

L

I

I

I

T

T

T

V

T

T

R

L

G

A

K

T

G

E

S

P

L

I

V

L

E

F

A

G

D

E

W

W

V

V

K

K

P

P

G

G

T

A

H

H

I

I

V

N

A

A

I

V

G

G

T

A

D

S

Q

R

R

P

G

D

K

W

Q

R

E

E

L

L

D

D

P

D

E

E

I

L

F

M

R

K

N

E

A

A

K

V

V

L

V

Y

V

V

D

D

S

S

V

Q

-

E

A

A

Q

A

C

L

T

K

E

E

K

S

G

G

E

D

T

V

W

-

H

-

P

-

L

-

N

-

K

-

N

-

I

L

I

L

T

S

K

G

D

A

D

E

I

I

H

F

G

A

E

E

I

L

G

G

E

E

V

V

L

I

L

K

G

G

R

V

K

K

P

P

G

A

R

H

E

-

S

-

D

C

D

E

E

K

I

T

T

T

I

V

F

F

D

K

S

S

T

L

G

G

M

M

A

A

I

V

Q

E

D

D

N

T

T

V

T

A

A

A

S

K

K

L

I

I

Y

Y

Q

D

N

S

GAGVQA 143:148 (≠ GTGNQA 138:143) binding
N168 (≠ S163) binding
R169 (= R164) binding

4mpdA Staphyloferrin b precursor biosynthetic enzyme sbnb bound a- ketoglutarate and NAD+ (see paper)
29% identity, 94% coverage: 21:329/330 of query aligns to 23:318/318 of 4mpdA

query
sites
4mpdA

I

V

G

D

A

A

V

L

E

T

E

E

A

A

Y

L

K

T

A

A

F

H

S

A

S

H

D

N

Q

D

V

F

E

V

Q

Q

P

P

D

-

Y

-

I

-

G

-

I

-

H

L

H
x
K

P

P

S

Y

L

L

R

R

G

-

E

-

I

-

D

-

F

-

K

-

L

-

G

-

Y

-

Y

-

K

Q

A

A

N

D

E
x
R

I

I

I
|
I

S

A

M
|
M

K

P

A

S

H

H

S

I

-

I

-

S

-

G

-

I

-
x
K

-

W

-
x
I

G

G

G
x
S

F

K

T

H

N

D

N

N

P

P

A

S

E

K

H

R

G

N

V

M

P

E

N
x
R

S

A

I

S

G

G

T

V

I

I

L

I

L

L

F

N

D

D

A

P

R

E

S

T

C

N

A

Y

L

P

I

I

C

A

I

V

M

M

D

E

G

A

S

S

L

L

I

I

T

S

G

S

L

M

R
|
R

T
|
T

G

A

A

A

S

V

G

S

A

V

V

I

S

A

V

A

K

K

A

H

L

L

A

A

R

K

K

K

N

G

A

F

R

K

T

D

V

L

A

T

S

I

I

I

G
|
G

T

C

G
|
G

N
x
L

Q
x
I

A

G

R

D

M

K

Q

Q

I

L

R

Q

A

S

V

M

N

L

E

E

I

Q

M

F

-

D

K

H

I

I

E

E

K

R

I

V

H

F

A

V

W

Y

S
x
D

R
x
Q

T
x
F

P

S

E

E

S

A

L

C

S

A

R

R

Y

F

K

V

T

D

D

R

I

W

Q

Q

R

Q

E

Q

F

R

G

P

-

E

I

I

P

N

V

F

I

I

V

A

A

T

S

E

S

N

K

A

K

K

E

E

A

A

V

V

E

S

Q

N

A

G

D

E

I

V

L

V

I

I

T

T

T
x
C

T
|
T

R
x
V

G
x
T

K

D

G

Q

S

P

L
x
Y

V

I

E

E

A

Y

D

D

W

W

V

L

K

Q

P

K

G

G

T

A

H

F

I

I

V

S

A

N

I
|
I

G
x
S

T

I

D

-

Q

-

R

-

G

-

K

-

Q
x
M

E

D

L

V

D

H

P

K

E

E

I

V

F

F

R

I

N

K

A

A

-

D

K

K

V

V

V

V

V

V

D

D

S

D

V

W

A

S

Q

Q

C

C

T

N

E

T

K

I

G

N

E

Q

T

L

W

-

H

-

P

-

L

V

N

L

K

E

N

G

I

K

I

F

T

S

K

K

D

E

D

A

I

L

H

H

G

A

E

E

I

L

G

G

E

Q

V

L

L

V

L

T

G

G

R

D

K

I

P

P

G

G

R

R

E

E

S

D

D

D

D

D

E

E

I

I

T

I

I

L

F

L

D

N

S
x
P

T
x
M

G
|
G

M

M

A

A

I

I

Q

E

D

D

N

I

T

S

T

S

A

A

S

Y

K

F

I

I

Y

Y

Q

Q

N

Q

A

A

I

Q

A

Q

N

Q

N

N

V

I

G

G

T

T

F

T

F

L

Q

N

F

L

F

Y

active site: I53 (= I66), M224 (≠ Q233)
binding 2-oxoglutaric acid: K43 (≠ H46), R51 (≠ E64), I53 (= I66), M55 (= M68), K64 (vs. gap), I66 (vs. gap), S68 (≠ G74), R80 (≠ N86), R108 (= R114), M289 (≠ T300), G290 (= G301)
binding nicotinamide-adenine-dinucleotide (acidic form): R51 (≠ E64), R80 (≠ N86), R108 (= R114), T109 (= T115), G132 (= G138), G134 (= G140), L135 (≠ N141), I136 (≠ Q142), D158 (≠ S163), Q159 (≠ R164), F160 (≠ T165), C199 (≠ T203), T200 (= T204), V201 (≠ R205), T202 (≠ G206), Y206 (≠ L210), I221 (= I225), S222 (≠ G226), P288 (≠ S299), M289 (≠ T300), G290 (= G301)

4bvaA Crystal structure of the NADPH-t3 form of mouse mu-crystallin. (see paper)
34% identity, 69% coverage: 89:316/330 of query aligns to 84:299/303 of 4bvaA

query
sites
4bvaA

G

A

T

S

I

V

L

L

L

L

F

F

D

D

A

P

R

S

S

N

C

G

A

S

L

L

I

L

C

A

I

V

M

M

D

D

G

G

S

N

L

V

I

I

T
|
T

G

A

L

K

R
|
R

T
|
T

G

A

A

A

S

V

G

S

A

A

V

I

S

A

V

T

K

K

A

L

L

L

A

K

R

P

K

P

N

G

A

S

R

D

T

V

V

L

A

C

S

I

I

L

G
|
G

T
x
A

G
|
G

N
x
V

Q
|
Q

A

A

R

Y

M

S

Q

H

I

Y

R

E

A

I

V

F

N

T

E

E

I

Q

M

F

K

S

I

F

E

K

K

E

I

V

H

R

A

M

W

W

S
x
N

R
|
R

T
|
T

P

R

E

E

S
x
N

L

A

S

E

R

K

Y

F

K

A

T

S

D

T

I

V

Q

Q

R

G

E

D

F

-

G

-

I

-

P

-

V

V

I

R

V

V

A

C

S

S

S

S

K

V

K

Q

E

E

A

A

V

V

E

T

Q

G

A

A

D

D

I

V

L

I

I

I

T

T

T
x
V

T
|
T

R
x
M

G
x
A

K

T

G

E

S

P

L

I

V

L

E

F

A

G

D

E

W

W

V

V

K

K

P

P

G

G

T

A

H

H

I

I

V

N

A

A

I
x
V

G
|
G

T

A

D
x
S

Q
x
R

R

P

G

D

K
x
W

Q

R

E

E

L

L

D

D

P

D

E

E

I

L

F

M

R

R

N

Q

A

A

K

V

V

L

V

Y

V

V

D

D

S

S

V

R

-

E

A

A

Q

A

C

L

T

K

E
|
E

K

S

G

G

E

D

T

V

W

-

H

-

P

-

L

-

N

-

K

-

N

-

I

L

I

L

T

S

K

G

D

A

D

D

I

I

H

F

G

A

E

E

I

L

G

G

E

E

V

V

L

I

L

S

G

G

R

A

K

K

P

P

G

A

R

H

E

-

S

-

D

C

D

E

E

K

I

T

T

T

I

V

F

F

D

K

S
|
S

T
x
L

G
|
G

M

M

A

A

I

V

Q

E

D

D

N

L

T

V

T

A

A

A

S

K

K

L

I

V

Y

Y

Q

D

N

S

active site: S219 (≠ D228)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T106 (= T111), R109 (= R114), T110 (= T115), G133 (= G138), A134 (≠ T139), G135 (= G140), V136 (≠ N141), Q137 (= Q142), N158 (≠ S163), R159 (= R164), T160 (= T165), N163 (≠ S168), V194 (≠ T203), T195 (= T204), M196 (≠ R205), A197 (≠ G206), V216 (≠ I225), G217 (= G226), S219 (≠ D228), S282 (= S299), L283 (≠ T300), G284 (= G301)
binding 3,5,3'triiodothyronine: S219 (≠ D228), R220 (≠ Q229), W223 (≠ K232), E247 (= E255)

Sites not aligning to the query:

active site: 59
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 82
binding 3,5,3'triiodothyronine: 46, 48, 57, 59, 76, 78, 82

4m54A The structure of the staphyloferrin b precursor biosynthetic enzyme sbnb bound to n-(1-amino-1-carboxyl-2-ethyl)-glutamic acid and nadh (see paper)
29% identity, 94% coverage: 21:329/330 of query aligns to 23:310/310 of 4m54A

query
sites
4m54A

I

V

G

D

A

A

V

L

E

T

E

E

A

A

Y

L

K

T

A

A

F

H

S

A

S

H

D

N

Q

D

V

F

E

V

Q

Q

P

P

D

-

Y

-

I

-

G

-

I

-

H

L

H

K

P

P

S
x
Y

L

L

R

R

G

Q

E

H

I

I

D

-

F

-

K

-

L

-

G

-

Y

-

Y

-

K

-

A

A

N

D

E
x
R

I

I

I
|
I

S

A

M
|
M

K

P

A

S

H

H

S

I

G

G

G

G

-

E

-

H

-

A

-

I

-

S

-

G

-

I

-
x
K

-

W

-
x
I

-

G

-

S

F

K

T

H

N

D

N

N

P

P

A

S

E

K

H

R

G

N

V

M

P

E

N
x
R

S
x
A

I

S

G

G

T

V

I

I

L

I

L

L

F

N

D

D

A

P

R

E

S

T

C

N

A

Y

L

P

I

I

C

A

I

V

M

M

D

E

G

A

S

S

L

L

I

I

T
x
S

G

S

L

M

R
|
R

T
|
T

G

A

A

A

S

V

G

S

A

V

V

I

S

A

V

A

K

K

A

H

L

L

A

A

R

K

K

K

N

G

A

F

R

K

T

D

V

L

A

T

S

I

I
|
I

G
|
G

T
x
C

G
|
G

N
x
L

Q
x
I

A

G

R

D

M

K

Q

Q

I

L

R

Q

A

S

V

M

N

L

E

E

I

Q

M

F

-

D

K

H

I

I

E

E

K

R

I

V

H

F

A

V

W

Y

S
x
D

R
x
Q

T

F

P

S

E

E

S

A

L

C

S

A

R

R

Y

F

K

V

T

D

D

R

I

W

Q

Q

R

Q

E

Q

F

R

G

P

-

E

I

I

P

N

V

F

I

I

V

A

A

T

S

E

S

N

K

A

K

K

E

E

A

A

V

V

E

S

Q

N

A

G

D

E

I

V

L

V

I

I

T

T

T
x
C

T
|
T

R
x
V

G
x
T

K

D

G

Q

S

P

L

Y

V

I

E

E

A

Y

D

D

W

W

V

L

K

Q

P

K

G

G

T

A

H

F

I

I

V

S

A

N

I
|
I

G
x
S

T

I

D

-

Q

-

R

-

G

-

K

-

Q
x
M

E

D

L

V

D

H

P

K

E

E

I

V

F

F

R

I

N

K

A

A

-

D

K

K

V

V

V

V

V

V

D

D

S

D

V

W

A

S

Q

Q

C

C

T

-

E

-

K

-

G

-

E

-

T

-

W

-

H

-

P

-

L

-

N

-

K

-

N

-

I

-

I

-

T

-

K

-

D

-

D

N

I

L

H

H

G

A

E

E

I

L

G

G

E

Q

V

L

L

V

L

T

G

G

R

D

K

I

P

P

G

G

R

R

E

E

S

D

D

D

D

D

E

E

I

I

T

I

I

L

F

L

D

N

S
x
P

T
x
M

G
|
G

M

M

A

A

I

I

Q

E

D

D

N

I

T

S

T

S

A

A

S

Y

K

F

I

I

Y

Y

Q

Q

N

Q

A

A

I

Q

A

Q

N

Q

N

N

V

I

G

G

T

T

F

T

F

L

Q

N

F

L

F

Y

active site: I55 (= I66), M231 (≠ Q233)
binding N-[(2S)-2-amino-2-carboxyethyl]-L-glutamic acid: Y45 (≠ S48), R53 (≠ E64), M57 (= M68), K71 (vs. gap), I73 (vs. gap), R87 (≠ N86), A88 (≠ S87), S112 (≠ T111), R115 (= R114), M281 (≠ T300), G282 (= G301)
binding 1,4-dihydronicotinamide adenine dinucleotide: R87 (≠ N86), R115 (= R114), T116 (= T115), I138 (= I137), G139 (= G138), C140 (≠ T139), G141 (= G140), L142 (≠ N141), I143 (≠ Q142), D165 (≠ S163), Q166 (≠ R164), C206 (≠ T203), T207 (= T204), V208 (≠ R205), T209 (≠ G206), I228 (= I225), S229 (≠ G226), P280 (≠ S299), M281 (≠ T300), G282 (= G301)

1u7hA Structure and a proposed mechanism for ornithine cyclodeaminase from pseudomonas putida (see paper)
33% identity, 71% coverage: 92:324/330 of query aligns to 89:317/341 of 1u7hA

query
sites
1u7hA

L

V

L

L

F

A

D

D

A

V

R

D

S

S

C

G

A

Y

L

P

I

V

C

L

I

L

M

S

D

E

G

L

S

T

L

I

I

A

T

T

G

A

L

L

R
|
R

T
|
T

G

A

A

A

S

T

G

S

A

L

V

M

S

A

V

A

K

Q

A

A

L

L

A

A

R

R

K

P

N

N

A

A

R

R

T

K

V

M

A

A

S

L

I

I

G
|
G

T

N

G
|
G

N
x
A

Q
|
Q

A

S

R

E

M

F

Q

Q

I

A

R

L

A

A

V

F

N

H

E

K

I

H

M

L

K

G

I

I

E

E

K

E

I

I

H

V

A

A

W
x
Y

S
x
D

R
x
T

T
x
D

P

P

E

L

S

A

L

T

S

A

R

K

Y

L

K

I

T

A

D

N

I

L

Q

K

R

E

E

Y

F

S

G

G

I

L

P

T

V

I

I

R

V

R

A

A

S

S

S

S

K

V

K

A

E

E

A

A

V

V

E

K

Q

G

A

V

D

D

I

I

L

I

I

T

T

T

T
x
V

T
|
T

R
x
A

G

D

K

K

-

A

-

Y

G

A

S

T

L
x
I

V

I

E

T

A

P

D

D

W

M

V

L

K

E

P

P

G

G

T

M

H

H

I

L

V

N

A

A

I
x
V

G
|
G

T

G

D
|
D

Q

C

R

P

G

G

K
|
K

Q

T

E

E

L

L

D

H

P

A

E

D

I

V

F

L

R

R

N

N

A

A

K

R

V

V

V

F

V

V

D

E

S

Y

V

E

A

P

Q

Q

C

T

T

R

E

I

K

E

G

G

E

E

T

I

W

Q

H

Q

-

L

P

P

L

A

N

D

K

F

N

P

I

V

I

V

T

-

K

-

D

-

D

-

I

-

H

-

G

-

E

D

I

L

G

W

E

R

V

V

L

L

L

R

G

G

R

E

K

T

P

E

G

G

R

R

E

Q

S

S

D

D

D

S

E

Q

I

V

T

T

I

V

F

F

D

D

S
|
S

T
x
V

G
|
G

M

F

A

A

I

L

Q

E

D

D

N

Y

T

T

T

V

A

L

S

R

K

Y

I

V

Y

L

Q

Q

N

Q

A

A

I

E

A

K

N

R

N

G

V

M

G

G

T

T

active site: D227 (= D228)
binding nicotinamide-adenine-dinucleotide: R111 (= R114), T112 (= T115), G135 (= G138), G137 (= G140), A138 (≠ N141), Q139 (= Q142), Y159 (≠ W162), D160 (≠ S163), T161 (≠ R164), D162 (≠ T165), V200 (≠ T203), T201 (= T204), A202 (≠ R205), I209 (≠ L210), V224 (≠ I225), G225 (= G226), D227 (= D228),